Why Nanoformulated Medicines?

  • Ultrasonic nanoemulsions excel as drug carrier due to a significantly higher solubilization capacity than simple micelle solutions.
  • Their thermodynamic stability offers advantages over unstable systems such as macro-sized emulsions, dispersions and suspensions.
  • Hielscher ultrasonicators are used to prepare nanoemulsions with droplets down to 10nm – in small scale and industrial production.

Pharmaceutical Nanoformulations Produced by Power Ultrasound

Since pharmacological effects are mostly related directly to the plasma levels, the absorption and bioavailability of active pharmaceutical ingredients is crucial. The bioavailability of phytochemical such as cannabinoids (i.e. CBD, THC, CBG and others) or curcuminoids is limited due to poor solubility, poor permeation, low systemic availability, instability, extensive first pass metabolism or degradation in the GI tract.
Nanoformulations such as nano-emulsions, liposomes, micelles, nano-crystals, or loaded nanoparticles are used in pharmaceuticals and supplements for improved and/or targeted drug delivery. Nanoemulsions are known to be very good vehicles to achieve a high bioavailability of active pharmaceutical ingredients (APIs) and phytochemical compounds. Furthermore, nanoemulsions may also protect APIs, which may be susceptible to hydrolysis and oxidation. APIs and phytochemicals (e.g. cannabinoids, curcuminoids) encapsulated in O/W nano-emulsions have been tested in various scientific trials and are well established as drug carriers with superior absorption rates.

n this video we make a nano-emulsion of CBD rich hemp oil in water using a Hielscher UP400St ultrasonicator. We then measure the nano-emulsion using a NANO-flex DLS. The measurement results show a very narrow, volume-weighed particle size distribution in the range from 9 to 40 nanometers. 95 percent of all particles are below 28 nanometers.

CBD Nanoemulsion - Produce a translucent nano-emulsion using a UP400St ultrasonic homogenizer!

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Ultrasonic preparation of nanosuspensions is an efficacious technique for the production of pharmaceutical formulations with increased bioavailability.

Ultrasonicator for the formulation of pharmaceutical nanosuspensions with increased bioavailbility.

Ultrasonic Nanoemulsification of Orally Delivered Drugs

The bioavailability of orally administered flavonoids as well as many other phenolic active ingredients is severely limited by extensive first-pass glucuronidation. In order to overcome the limitations of poor bioavailability, nano-sized carriers such as nanoemulsions and liposomes have been extensively evaluated for various drugs and have shown great results in improving the absorption.
Paclitaxel: Nanoemulsions loaded with paclitaxel (a chemotherapy drug used in cancer treatments) had a droplet size between ~90.6nm (smallest mean particle size) and 110nm.
“The results of the pharmacokinetic studies indicated that encapsulation of paclitaxel in nanoemulsions did enhance the oral bioavailability of paclitaxel significantly. The enhanced oral bioavailability, as measured by the area-under-the curve (AUC), of paclitaxel in nanoemulsions might be attributed to the solubilization of the drug in the oil droplets and/or to the presence of surfactants at the oil–water interface. Enhanced absorption of paclitaxel can also be attributed to the protection of drug from chemical as well as enzymatic degradation. Improved oral bioavailability of various hydrophobic drugs in O/W type of emulsions has been reported in the literature.” [Tiwari 2006, 445]

Curcuminoids: Lu et al. (2017, p.53) report the preparation of ultrasonically extracted curcuminoids, which have been emulsified ultrasonically into a nanoemulsion. Curcuminoids were extracted under sonication in ethanol. For the nano-emulsification, they put 5mL curcuminoid extract into a vial and evaporated the ethanol under nitrogen. Then, 0.75 g of lecithin and 1 mL of Tween 80 were added and mixed homogeneously, which was followed by adding 5.3mL of deionized water. The mixture was stirred thoroughly and sonicated afterwards.
The ultrasonic nanoemulsification resulted in an uniform curcuminoid nanoemulsion with an average particle size of 12.1 nanometers and a spherical shape, which was determined by TEM (see figure below).

Determination of oral bioavailability of ultrasonic curcuminoid dispersions and nanoemulsions prepared from Curcuma longa Linnaeus.

Fig.: DLS particle size distribution (A) and TEM image (B) of curcuminoids dispersion along with particle size distribution obtained directly from the TEM image (C).
(Picture and study: © Lu et al., 2017)

Polymers such as polylactic-co-glycolic acid (PLGA) or polyethylene glycol are often used as a major component to improve encapsulation and enhancement of both stability and oral bioavailability. However, the use of polymers is correlated with a larger particle size (often >100nm). The prepared curcuminoid nanoemulsion by Lu et al. had a substantially reduced size of 12-16nm. The shelf-life was also improved with a high stability of our curcuminoid nanoemulsion over a storage period of 6 months at 4℃ and 25℃ as indicated by a mean particle size of 12.4 ± 0.5nm and 16.7 ± 0.6nm, respectively, after prolonged storage.

Ultrasonic glass flow reactors are used in lab and industrial setting for emulsification, dispersion, homogenisation, mixing, extraction, disintegration, and sonochemical reactions (e.g., sono-synthesis, sono-catalysis)

Ultrasonic glass flow cell for inline nano-emulsification

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The Effect of Pharmaceutical Excipients and Ultrasonic Nanoemulsification

Dong et al. investigated 21 pharmaceutical excipients and their effects on the bioavailability of the model flavonoid chrysin. Five excipients – namely Brij 35, Brij 58, labrasol, sodium oleate, and Tween20 significantly inhibited chrysin glucuronidation. Sodium oleate was the most potent inhibitor of glucuronidation.

Mebudipine: Khani et al. (2016) report the formulation of a mebudipine-loaded nanoemulsion containing ethyl oleate, Tween 80, Span 80, polyethylene glycol 400, ethanol and DI water were prepared using probe-type ultrasonicator. They found the particle size for a optimum formulation was 22.8 ± 4.0 nm which resulted in a relative bioavailability of the mebudipine nanoemulsion that was enhanced by about 2.6-fold. The results of in vivo experiments have shown that nanoemulsion formulation was able to enhance bioavailability of mebudipine significantly compared to the suspension, oil-soluble and micellar solution.

Ultrasonic Nanoemulsion for Ocular Drug Delivery

Ocular nanoemulsions, e.g. for ophthalmic drug delivery, have been prepared in order to achieve better availability, faster penetration and higher efficacy.
Ammar et al (2009) formulated dorzolamide hydrochloride in a nanoemulsion (size range of 8.4-12.8nm) in order to achieve a increased effects in the treatment of glaucoma, a decrease in the number of applications per day, and a better patient compliance compared to conventional eye drops. The the developed nanoemulsions showed fast onset of drug action and prolonged effect as well as enhanced drug bioavailability compared to the conventional market product.
of high therapeutic efficacy

Morsi et al. (2014) prepared acetazolamide-loaded nanoemulsions as follows: 1% w/w acetazolamide (ACZ) was sonicated with surfactant / co-surfactant / oil blends until complete dissolution of the drug then the aqueous phase containing 3% w/w dimethyl sulfoxide (DMSO) was added dropwise to prepare nanoemulsions containing 39% w/w aqueous phase, while to prepare nanoemulsions at 59% water content, aqueous phase containing 20% DMSO was used. DMSO was added in order to prevent any precipitation of the drug after the addition of the aqueous phase. Nanoemulsions were prepared with a mean droplet size of of 23.8-90.2nm. The nanoemulsions prepared with a higher water content of 59% showed the highest drug release.
The nano-emulsified acetazolamide was successfully formulated in nanoemulsion form which revealed high therapeutic efficacy in treatment of glaucoma together with prolonged effect.

High Performance Ultrasonicators for Nano-Emulsification and Nanoencapsulation

Hielscher Ultrasonics offers ultrasonic systems from compact lab homogenizers to industrial turnkey solutions. To produce nanoemulsions of highest pharmaceutical grade, a reliable emulsification process is crucial. Hielscher’s broad variety of sonotrodes, flow cells and the optional insert MultiPhase Cavitator MPC48 enable our customer to setup the optimal processing conditions to produce nano-sized emulsions at standardized, reliable and consistent quality. Hielscher ultrasoniators are equipped with state-of-the-art software for operation and control – ensuring the reliable production of standardised pharmaceuticals and pharma-grade supplements.
Contact us today to discover the possibilities of ultrasonically nano-formulated APIs and phytochemicals!

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Ultrasonic high-shear homogenizers are used in lab, bench-top, pilot and industrial processing.

Hielscher Ultrasonics manufactures high-performance ultrasonic homogenizers for mixing applications, dispersion, emulsification and extraction on lab, pilot and industrial scale.


  • M.E. Barbinta-Patrascu, N. Badea, M. Constantin, C. Ungureanu, C. Nichita, S.M. Iordache, A. Vlad, S. Antohe (2018): Bio-Activity of Organic/Inorganic Photo-Generated Composites in Bio-Inspired Systems. Romanian Journal of Physics 63, 702 (2018).
  • Raquel Martínez-González, Joan Estelrich, Maria Antònia Busquets (2016): Liposomes Loaded with Hydrophobic Iron Oxide Nanoparticles: Suitable T2 Contrast Agents for MRI. International Journal of Molecular Science 2016.
  • Shah Purvin, Parameswara Rao Vuddanda, Sanjay Kumar Singh, Achint Jain, and Sanjay Singh (2014): Pharmacokinetic and Tissue Distribution Study of Solid Lipid Nanoparticles of Zidov in Rats. Journal of Nanotechnology, Volume 2014.
  • Harshita Krishnatreyya, Sanjay Dey, Paulami Pal, Pranab Jyoti Das, Vipin Kumar Sharma, Bhaskar Mazumder (2019): Piroxicam Loaded Solid Lipid Nanoparticles (SLNs): Potential for Topical Delivery. Indian Journal of Pharmaceutical Education and Research Vol 53, Issue 2, 2019. 82-92.
  • Ammar H. et al. (2009): Nanoemulsion as a Potential Ophthalmic Delivery System for Dorzolamide Hydrochloride. AAPS Pharm Sci Tech. 2009 Sep; 10(3): 808.
  • Dong D. et al. (2017): Sodium Oleate-Based Nanoemulsion Enhances Oral Absorption of Chrysin through Inhibition of UGT-Mediated Metabolism. Mol. Pharmaceutics, 2017, 14 (9). 2864–2874.
  • Gunasekaran Th. et al. (2014): Nanotechnology: an effective tool for enhancing bioavailability and bioactivity of phytomedicine. Asian Pac J Trop Biomed 2014; 4(Suppl 1). S1-S7.
  • Khani S. et al. (2016): Design and evaluation of oral nanoemulsion drug delivery system of mebudipine, Drug Delivery, 23:6, 2035-2043.
  • Lu P.S. et al. (2018): Determination of oral bioavailability of curcuminoid dispersions and nanoemulsions prepared from Curcuma longa Linnaeus. J Sci Food Agric 2018; 98: 51–63.
  • Morsi N.M. et al. (2014): Nanoemulsion as a Novel Ophthalmic Delivery System for Acetazolamide. International Journal of Pharmacy and Pharmaceutical Sciences Vol 6, Issue 11, 2014.
  • Tiwari S.B. et al (2006): Nanoemulsion Formulations for Improved Oral Delivery of Poorly Soluble Drugs. NSTI-Nanotech 2006.

Facts Worth Knowing

Ultrasonic Extraction of Active Compounds from Plants

High-power ultrasound is widely used to isolate from phytochemicals (i.e flavonoids, terpenes antioxidants etc.) from plant material. Ultrasonic cavitation perforates and breaks the cell walls so that the intracellular matter is released into the surrounding solvent. The big advantages of sonication lie the non-thermal treatment and the solvent use. Ultrasonic extraction is a non-thermal, mechanical method – meaning that the delicate phytochemicals are not degraded by high temperatures. Regarding solvents, there is a broad selection that can be used for extraction. Common solvents include water, ethanol, glycerin, vegetable oils (e.g. olive oil, MCT oils, coconut oil), grain alcohol (spirits), or a water-ethanol mix amongst may other solvents.
Click here to learn more about the ultrasonic extraction of phytochemical compounds from plants!

Entourage Effect

The extraction of a combination of several phytochemicals from a plant is known for stronger effects. The synergy of various plant compounds is known as entourage. Whole plant extracts combine manifold phytochemicals. For instance, cannabis contains over 480 active compounds. A cannabis extract, which includes CBD (cannabidiol), CBG (cannabigerol), CBN (cannabinol), CBC (cannabichromene), terpenes and many other phenolic compounds, is much more effective since the manifold compounds work synergistically. Ultrasonic extraction is a highly efficient method to produce a full spectrum extract of superior quality.

High performance ultrasonics! Hielscher's product range covers the full spectrum from the compact lab ultrasonicator over bench-top units to full-industrial ultrasonic systems.

Hielscher Ultrasonics manufactures high-performance ultrasonic homogenizers from lab to industrial size.

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