Why Nanoformulated Medicines?

• Ultrasonic nanoemulsions excel as drug carrier due to a significantly higher solubilization capacity than simple micelle solutions.
• Their thermodynamic stability offers advantages over unstable systems such as macro-sized emulsions, dispersions and suspensions.
• Hielscher ultrasonicators are used to prepare nanoemulsions with droplets down to 10nm – in small scale and industrial production.

Pharmaceutical Nanoformulations Produced by Power Ultrasound

Since pharmacological effects are mostly related directly to the plasma levels, the absorption and bioavailability of active pharmaceutical ingredients is crucial. The bioavailability of phytochemical such as cannabinoids (i.e. CBD, THC, CBG and others) or curcuminoids is limited due to poor solubility, poor permeation, low systemic availability, instability, extensive first pass metabolism or degradation in the GI tract.
Nanoformulations such as nano-emulsions, liposomes, micelles, nano-crystals, or loaded nanoparticles are used in pharmaceuticals and supplements for improved and/or targeted drug delivery. Nanoemulsions are known to be very good vehicles to achieve a high bioavailability of active pharmaceutical ingredients (APIs) and phytochemical compounds. Furthermore, nanoemulsions may also protect APIs, which may be susceptible to hydrolysis and oxidation. APIs and phytochemicals (e.g. cannabinoids, curcuminoids) encapsulated in O/W nano-emulsions have been tested in various scientific trials and are well established as drug carriers with superior absorption rates.

Ultrasonic Nanoemulsification of Orally Delivered Drugs

The bioavailability of orally administered flavonoids as well as many other phenolic active ingredients is severely limited by extensive first-pass glucuronidation. In order to overcome the limitations of poor bioavailability, nano-sized carriers such as nanoemulsions and liposomes have been extensively evaluated for various drugs and have shown great results in improving the absorption.
Paclitaxel: Nanoemulsions loaded with paclitaxel (a chemotherapy drug used in cancer treatments) had a droplet size between ~90.6nm (smallest mean particle size) and 110nm.
“The results of the pharmacokinetic studies indicated that encapsulation of paclitaxel in nanoemulsions did enhance the oral bioavailability of paclitaxel significantly. The enhanced oral bioavailability, as measured by the area-under-the curve (AUC), of paclitaxel in nanoemulsions might be attributed to the solubilization of the drug in the oil droplets and/or to the presence of surfactants at the oil–water interface. Enhanced absorption of paclitaxel can also be attributed to the protection of drug from chemical as well as enzymatic degradation. Improved oral bioavailability of various hydrophobic drugs in O/W type of emulsions has been reported in the literature.” [Tiwari 2006, 445]

Curcuminoids: Lu et al. (2017, p.53) report the preparation of ultrasonically extracted curcuminoids, which have been emulsified ultrasonically into a nanoemulsion. Curcuminoids were extracted under sonication in ethanol. For the nano-emulsification, they put 5mL curcuminoid extract into a vial and evaporated the ethanol under nitrogen. Then, 0.75 g of lecithin and 1 mL of Tween 80 were added and mixed homogeneously, which was followed by adding 5.3mL of deionized water. The mixture was stirred thoroughly and sonicated afterwards.
The ultrasonic nanoemulsification resulted in an uniform curcuminoid nanoemulsion with an average particle size of 12.1 nanometers and a spherical shape, which was determined by TEM (see figure below).

Fig.: DLS particle size distribution (A) and TEM image (B) of curcuminoids dispersion along with particle size distribution obtained directly from the TEM image (C).
(Picture and study: © Lu et al., 2017)

Polymers such as polylactic-co-glycolic acid (PLGA) or polyethylene glycol are often used as a major component to improve encapsulation and enhancement of both stability and oral bioavailability. However, the use of polymers is correlated with a larger particle size (often >100nm). The prepared curcuminoid nanoemulsion by Lu et al. had a substantially reduced size of 12-16nm. The shelf-life was also improved with a high stability of our curcuminoid nanoemulsion over a storage period of 6 months at 4℃ and 25℃ as indicated by a mean particle size of 12.4 ± 0.5nm and 16.7 ± 0.6nm, respectively, after prolonged storage.

The Effect of Pharmaceutical Excipients and Ultrasonic Nanoemulsification

Dong et al. investigated 21 pharmaceutical excipients and their effects on the bioavailability of the model flavonoid chrysin. Five excipients – namely Brij 35, Brij 58, labrasol, sodium oleate, and Tween20 significantly inhibited chrysin glucuronidation. Sodium oleate was the most potent inhibitor of glucuronidation.

Mebudipine: Khani et al. (2016) report the formulation of a mebudipine-loaded nanoemulsion containing ethyl oleate, Tween 80, Span 80, polyethylene glycol 400, ethanol and DI water were prepared using probe-type ultrasonicator. They found the particle size for a optimum formulation was 22.8 ± 4.0 nm which resulted in a relative bioavailability of the mebudipine nanoemulsion that was enhanced by about 2.6-fold. The results of in vivo experiments have shown that nanoemulsion formulation was able to enhance bioavailability of mebudipine significantly compared to the suspension, oil-soluble and micellar solution.

Ultrasonic Nanoemulsion for Ocular Drug Delivery

Ocular nanoemulsions, e.g. for ophthalmic drug delivery, have been prepared in order to achieve better availability, faster penetration and higher efficacy.
Ammar et al (2009) formulated dorzolamide hydrochloride in a nanoemulsion (size range of 8.4-12.8nm) in order to achieve a increased effects in the treatment of glaucoma, a decrease in the number of applications per day, and a better patient compliance compared to conventional eye drops. The the developed nanoemulsions showed fast onset of drug action and prolonged effect as well as enhanced drug bioavailability compared to the conventional market product.
of high therapeutic efficacy

Morsi et al. (2014) prepared acetazolamide-loaded nanoemulsions as follows: 1% w/w acetazolamide (ACZ) was sonicated with surfactant / co-surfactant / oil blends until complete dissolution of the drug then the aqueous phase containing 3% w/w dimethyl sulfoxide (DMSO) was added dropwise to prepare nanoemulsions containing 39% w/w aqueous phase, while to prepare nanoemulsions at 59% water content, aqueous phase containing 20% DMSO was used. DMSO was added in order to prevent any precipitation of the drug after the addition of the aqueous phase. Nanoemulsions were prepared with a mean droplet size of of 23.8-90.2nm. The nanoemulsions prepared with a higher water content of 59% showed the highest drug release.
The nano-emulsified acetazolamide was successfully formulated in nanoemulsion form which revealed high therapeutic efficacy in treatment of glaucoma together with prolonged effect.

High Performance Ultrasonicators for Nano-Emulsification and Nanoencapsulation

Hielscher Ultrasonics offers ultrasonic systems from compact lab homogenizers to industrial turnkey solutions. To produce nanoemulsions of highest pharmaceutical grade, a reliable emulsification process is crucial. Hielscher’s broad variety of sonotrodes, flow cells and the optional insert MultiPhase Cavitator MPC48 enable our customer to setup the optimal processing conditions to produce nano-sized emulsions at standardized, reliable and consistent quality. Hielscher ultrasoniators are equipped with state-of-the-art software for operation and control – ensuring the reliable production of standardised pharmaceuticals and pharma-grade supplements.
Contact us today to discover the possibilities of ultrasonically nano-formulated APIs and phytochemicals!