In laboratories, it is often necessary to prepare multiple samples simultaneously at the same process conditions. The VialTweeter enables for the simultaneous sonication of up to 10 vials at the same intensity. Thereby, the VialTweeter is a reliable lab homogenizer for homogenization, emulsification, dispersing and deagglomeration, extraction, lysis, dissolve, as well as the degassing of fluids. Due to the principle of indirect sonication, no probe is immersed into the sample so that cross-contamination and sample loss is completely avoided.

In the lab, often multiple samples of small volumes have to be prepared simultaneously – in order to mix, homogenize, emulsify, disperse, disintegrate or degas a liquid medium. The VialTweeter provides high power ultrasound to fulfill the required application by introducing ultrasonic cavitation indirectly through the vessel’s wall into up to 10 samples at the same time. Thereby, cross-contamination and sample loss are completely avoided.
In combination with the digital ultrasonic processor UP200St, the VialTweeter allows for the effective and comfortable sonication of up to 10 vials simultaneously and without cross-contamination. As the same ultrasound intensity is delivered to each sample, the sonication results are even and reproducible.

While ultrasonic cleaning baths and tanks provide only low ultrasound energy resulting in incomplete sample preparation results, the VialTweeter transmits intense ultrasonic forces through the vessels into the sample. During the indirect sonication, the test tubes remain permanently closed so that the sample cannot be contaminated, spoiled or volatilize. Sample losses are avoided, too.
Many standard test vials, such as auto-sampler vials, micro-centrifuge vials, reagent vials such as Eppendorf tubes or Nunc tubes of 1 to 5 mL fit into the VialTweeter. To provide even more flexibility, the adjustable and removable VialPress allows to press larger sample vessels to the front surface of the VialTweeter block sonotrode. Thereby, up to 5 larger vials can be sonicated indirectly at the same time.
 
 
Advantages of the VialTweeter at a glance

• Intense sonication of up to 10 vials simultaneously
• Indirect sonication at high ultrasonic intensity through vessel wall into the sample
• Indirect sonication avoids cross-contamination and sample loss
• Reproducible results due to adjustable and controllable sonication amplitude
• The VialPress enables you to sonicate larger tubes
• Adjustable pulse mode from 0 to 100%
• Autoclavable

 
The VialTweeter Setup consists in three major components, which are easy to mount and provide high user-friendliness and work comfort: The ultrasonic processor UP200St-G, the transducer UP200St-T and the VialTweeter.
The 200 watts ultrasonic processor is the ultrasonic driver of the VialTweeter. Due to its 200 watts of ultrasound power, it becomes possible to sonicate up to 10 vials at an ultrasonic intensity of up to 10 watts per each test tube. This makes the VialTweeter a very powerful and reliable work unit.

About the UP200St Core Unit

The UP200St offers so many applications due to the manifold available accessories. The core unit UP200St can be used as conventional probe ultrasonicator, too. Just dismount the VialTweeter block sonotrode and use one of the various sonotrodes, which are available for the UP200St.
This allows the user to change easy and quickly between indirect and direct sonication of very small up to mid-size samples. With its 200 watts ultrasound power, the UP200St handles effortlessly volumes from 0.1mL to 1000mL.

From the user aspect, the color touch display, the browser remote control, the built-in network as well as the automatic data recording on an integrated SD-Card are the most outstanding features, which allow for reliable, successful and comfortable ultrasonication.

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The Ultrasonic VialTweeter in Research and Science

The VialTweeter is a 200 watts powerful ultrasonic processor, that is ideal for simultaneous ultrasonic sample preparation of multiple Eppendorf vials or similar test tubes. Therefore, the VialTweeter is frequently used in biological and biochemical laboratories for research and life science. Below you can find a selection of scientific articles featuring the ultrasonic processor VialTweeter. The articles cover various applications such as from ultrasonic sample homogenization, cell disruption and lysis, DNA shearing and fragmentation, extraction of proteins and bioactive compounds as well as the inactivation of coronavirus SARS-CoV-2.
For alpha-synuclein fibril fragmentation, used e.g. in Parkinson research, the VialTweeter is the established sonicator for the efficient and reliable α-syn fragmentation. Find protocols and references here!
If you are looking for a specific application and related scientific references, please contact us!
 

• Gajek, Ryszard; Barley, Frank; She, Jianwen (2013): Determination of essential and toxic metals in blood by ICP-MS with calibration in synthetic matrix. Analytical Methods 5, 2013. 2193-2202.
  https://pubs.rsc.org/en/content/articlelanding/2013/ay/c3ay26036d
• Nordenfelt P, Waldemarson S, Linder A, Mörgelin M, Karlsson C, Malmström J, Björck L. (2012): Antibody orientation at bacterial surfaces is related to invasive infection. Journal of Experimental Medicine 17;209(13), 2012. 2367-81.
• Wenzel, M., A. I. Chiriac, A. Otto, D. Zweytick, C. May, C. Schumacher, R. Gust, et al. (2014): Small Cationic Antimicrobial Peptides Delocalize Peripheral Membrane Proteins. Proceedings of the National Academy of Sciences 111, No. 14, 2014. E1409–E1418.
• Lindemann, C., Lupilova, N., Müller, A., Warscheid, B., Meyer, H. E., Kuhlmann, K., Eisenacher, M., Leichert, L. I. (2013): Redox proteomics uncovers peroxynitrite-sensitive proteins that help Escherichia coli to overcome nitrosative stress. The Journal of biological chemistry, 288(27), 2013. 19698–19714.
• Wenzel, M., Patra, M., Albrecht, D., Chen, D. Y., Nicolaou, K. C., Metzler-Nolte, N., Bandow, J. E. (2011): Proteomic signature of fatty acid biosynthesis inhibition available for in vivo mechanism-of-action studies. Antimicrobial agents and chemotherapy, 55(6), 2011. 2590–2596.
• Laughton, S., Laycock, A., von der Kammer, F. et al. (2019): Persistence of copper-based nanoparticle-containing foliar sprays in Lactuca sativa (lettuce) characterized by spICP-MS. Journal of Nanoparticle Research 21, 174 (2019).
• Welch, Stephen R.; Davies, Katherine A.; Buczkowski, Hubert; Hettiarachchi, Nipunadi; Green, Nicole; Arnold, Ulrike; Jones, Matthew; Hannah, Matthew J.; Evans, Reah; Burton, Christopher; Burton, Jane E.; Guiver, Malcolm; Cane, Patricia A.; Woodford, Neil; Bruce, Christine B.; Roberts, Allen D. G.; Killip, Marian J. (2020): Inactivation analysis of SARS-CoV-2 by specimen transport media, nucleic acid extraction reagents, detergents and fixatives. Journal of Clinical Microbiology. Accepted Manuscript Posted Online 24 August 2020.