96-Well Plate Sonicator UIP400MTP for High-Throughput Sample Prep
96-well plates, microplates, multi-well plates, microtiter plates or ELISA plates are used for the mass sample cultivation and processing of biological samples. With the microtiter plate ultrasonicator UIP400MTP, Hielscher offers a unique ultrasonic solution of the uniform and reliable sample preparation of 12-, 24,-, 48-, 96-, 384-, or 1536-well plates. Common applications of the microtiter ultrasonicator UIP400MTP include cell lysis, protein extraction, cell homogenization and solubilization as well as DNA fragmentation.
- Uniform sonication of 1 – 1536 samples
- 400 watts ultrasound power – precisely controllable
- For cell cultures, FFPE and frozen tissue
- Compatible with any standard multi-well plate
- One-time investment at a highly reasonable price
- Use you own plates! No proprietary multiwell plates or consumables required.
- Works also with tubes and petri dishes
- Simultaneous mass sample preparation
- Precisely controllable sonication parameters (amplitude, duration, cycle, temperature)
- Reproducible, repeatable results
- Convenient and safe operation
- Suitable for automation
Multi-Sample Sonicator UIP400MTP
Mass Sample Preparation with the Ultrasonicator UIP400MTP
96-well plates and microplates are tools for the simultaneously handling and processing a large number of samples. As its name “96-well plate” already indicates, the 96-well microplate has 96 wells and can hold thereby 96 individual samples. Sample preparation before analysis, e.g. before cell-based assays (such as ELISA or PCR) requires cell lysis, cell disruption as well as protein extraction and releasing other targeted intracellular molecules. Ultrasonic cell lysis and extraction is a long-time and well established method of high-quality sample preparation. Ultrasonic cell lysis and protein extraction is performed in accordance to protocols and gives thereby repeatable and reliable results. The mass-sample ultrasonicator UIP400MTP is a 400 watts powerful ultrasonic system that couples the ultrasonic waves indirectly through the walls of the multi-well plates into the sample medium. Each of the 96 sample wells is sonicated with the exactly same ultrasonic intensity resulting in superior cell lysis and extraction yields.
Applications of the UIP400MTP High-Throughput Ultrasonicator
The UIP400MTP ultrasonicator fulfills numerous applications based on the type of sample. The following overview introduces you to the capabilities of the Hielscher UIP400MTP sonicator for sample preparation in biology, life science, genomics, proteomics and biomarker diagnostics.
- Cell Lysis
Mammalian Cell Lysis
Plant Cell Lysis
Bacterial Cell Lysis
Yeast Cell Lysis - Tissue Lysis and Extraction
Fresh Frozen Tissue Lysis
Formalin-Fixed, Paraffin-Embedded (FFPE) Tissue Extraction - DNA, RNA, and Total Nucleic Acid (tNA) Extraction
DNA Extraction
RNA Extraction
Total Nucleic Acid (tNA) Extraction - Protein Extraction
- Chromatin Shearing
DNA Shearing
RNA Shearing - Cell-Free DNA (cfDNA) Extraction from Plasma
- Extraction from Cell Cultures
Extraction from Mammalian Cell Cultures
Extraction from Plant Cell Cultures
Extraction from Bacterial Cell Cultures
Extraction from Yeast Cell Cultures
The Advantages of Lysate Preparation using the Microplate Sonicator UIP400MTP
The multi-well plate sonicator UIP400MTP offers numerous benefits.
Lysate preparation in 96-well plates, microplates and multiwell plates (96-, 384- ,1536-well plates) can present a few challenges. During lysate preparation in 96-well plates and other micro-plates, several challenges can arise. These challenges include inconsistent sample disruption, limited scalability, cross-contamination, and labor-intensive processes. With the 96-well plate sonicator UIP400MTP, Hielscher has designed a reliable and comfortable lysate prep system. Developed specifically for multiwell-plate lysate preparation, the microplate sonicator UIP400MTP offers unique advantages over traditional techniques such as plate shakers and bead mill lysing. Thereby, the 96-well plate sonicator UIP400MTP overcomes these challenges in multiwell-plate sample preparation and helps you to achieve high-throughput lysis with excellent uniformity, reproducibility, and user-comfort.
Below, we address each problem and how the UIP400MTP microplate-sonicator helps to overcome these challenges:
Microtiter plate sonicator UIP400MTP is used to dislodge biofilms for research
| Problem Description | Solution with the UIP400MTP |
|---|---|
| Problem of Inconsistent Sample Disruption: Achieving consistent and efficient sample disruption in each well of a multiwell plate can be difficult. Traditional methods for lysate preparation like plate shakers or bead beating/milling often do not achieve uniform disruption across all wells. This inconsistency can result in variations in lysate quality and impact downstream applications. |
The Microplate Sonicator Solution: The Hielscher microplate sonicator UIP400MTP utilizes high-intensity ultrasound waves to generate cavitation, uniformly disrupting samples in each well. The 96-well plates and multiwell plates are uniformly agitated, ensuring consistent and reliable lysate preparation across the entire plate. |
| Problem of Scalability: Sample processing in 96-well plates or other multiwell plates often requires scalability for higher throughput. Plate shakers and bead mills can be limited in scalability due to the size and number of plates they can accommodate. |
The 96-Well Plate Sonicator Solution: The UIP400MTP allows the use of standard plates with 12-, 24-, 48-, 96-, 384-, or 1536-wells, enabling quick scale-up to large-throughput sample preparation. The compact design allows multiple sonicators to be clustered side by side for simultaneous sonication of multiple plates, increasing throughput and productivity. |
| Problem of Cross-Contamination: Cross-contamination is a common concern when working with multiwell plates. Traditional methods may lead to sample splashing or carryover between wells, compromising data integrity. |
The 96-Well Plate Sonicator Solution: The UIP400MTP indirectly sonicates the well-plates, eliminating the need for beads or chemicals. Plates can be covered with a foil or lid to create a closed system, effectively preventing cross-contamination and ensuring reliable sample processing. |
| Problem of Expensive Proprietary Disposables: Many high-throughput sonicators require expensive proprietary multiwell plates, limiting flexibility and increasing costs. |
The 96-Well Plate Sonicator Solution: The UIP400MTP works with any standard 96-well plates or multi-wells, giving users the freedom to choose the most suitable and economical plates for their research, saving money and enhancing flexibility. |
| Problem of Labor-Intensive Processes: Lysate preparation in multiwell plates can be labor-intensive, requiring repetitive manual steps and prolonged processing times. |
The 96-Well Plate Sonicator Solution: The UIP400MTP streamlines the process by simultaneously treating all wells, reducing hands-on time and manual steps. Specific sonication protocols can be saved, enabling reproducible and efficient processing. |
| Problem of Sample Recovery: Traditional methods like bead mills can result in poor sample recovery due to sample loss during bead transfer or difficulties in separating samples from beads. |
The 96-Well Plate Sonicator Solution: The UIP400MTP eliminates the need for beads, allowing for efficient sample recovery with no contamination or loss. Samples can be directly processed or passed to subsequent steps without additional separation steps. |
| Problem of Non-Versatile Use: Conventional methods lack flexibility in accommodating changes in sample volumes, plate types, or varying treatment intensities. |
The 96-Well Plate Sonicator Solution: The UIP400MTP supports various plate formats and customizable sonication parameters, providing maximum versatility for different sample types and applications. |
| Problem of Compatibility with Automation: Bead-milling or enzymatic/chemical lysis requires additional post-processing steps that disrupt automated workflows. |
The 96-Well Plate Sonicator Solution: The UIP400MTP integrates seamlessly into auto-sampler lines, enabling reliable and repetitive sample preparation for standardized and automated workflows. |
Multi-well plate sonicator UIP400MTP for high-throughput sample preparation
Typical Applications:
Uniform Sonication of All Wells with the UIP400MTP
Tests such as the aluminium foil test and the emulsion test prove the uniform ultrasound intensity over the complete surface of the UIP4000MTP. The uniform intensity result in homogeneous sample sonication in all wells of the multi-well plates.
Aluminium Foil Test with the UIP400MTP
The aluminium foil test performed with the multi-well plate ultrasonicator UIP400MTP demonstrates the uniform generation of acoustic cavitation over the complete surface.
The complete surface of the aluminium foil shows uniformly distributed holes caused by cavitation.
The aluminium foil test demonstrates the uniform occurrence of acoustic cavitation over the complete surface of the UIP400MTP.
Emulsion Test with the UIP400MTP
The results of the emulsion test visually demonstrate the uniform ultrasonic intensity of the UIP400MTP. The upper picture shows the clear water-oil mixture in a 96-well plate before sonication. The second picture shows the ultrasonic emulsions after 1 min. treatment. All wells show the same loss of transparency, which indicates a uniform emulsion in all cavities.
The emulsion test of oil and water in the cavities of a 96-well plate demonstrate the uniform ultrasonic intensity over the complete plate. After sonication, all wells show the same emulsification degree indicated by the same transparency loss in all wells. (Picture above: unsonicated oil and water; picture below: sonicated O/W emulsions)
Precise Temperature Control During Microtiter Plate Sonication
The UIP400MTP is a 400 watts powerful ultrasonicator, which transmits ultrasound waves and vibrations indirectly through the walls of the microtiter plate into the samples and creates cavitation and shear forces in the medium. As all mechanical cell disruption methods, sonication creates heat. However, in contrast to alternative cell disruption methods, the UIP400MTP allows for sophisticated temperature monitoring and pre-setting of temperature limits to prevent any thermal degradation of the biological samples.
The UIP400MTP is equipped with a smart software. Via the digital touch screen, the menu can be easily accessed to pre-set temperature limits and to program the total energy input for your sample run.
Monitoring the sample temperature: The MultiSample Ultrasonicator UIP400MTP is equipped with an intelligent software and a pluggable temperature sensor. Plug the temperature sensor into the UIP400MTP and insert the tip of the temperature sensor in one of the microtiter wells. Via digital coloured touch-display, you can set in the menu of the UIP400MTP a specific temperature range for your sample sonication. The ultrasonicator will automatically stop when the max temperature is reached and pause until the sample temperature is down to the lower value of the set temperature ∆. Then the sonication starts automatically again. This smart feature prevents heat-induced degradation.
If possible, you might pre-cool the 96-well plate or microtiter plate and its samples to postpone reaching the critical temperature limit.
The UIP400MTP is suitable for the sonication of any standard 96-well plates, microplates, microtiter plates and multiwell plates.
“I am writing to thank you for our fantastic experience using your UIP400MTP sonicator for uniform and consistent bacterial lysis of fecal microbiome samples. We are pushing the envelope for high throughput lysis technology, and your instrument is well suited for the task. Some of the key highlights are:
- Ease of operation- instrument is easy to use, and customization is straightforward using the menu choices
- High throughput- excellent for handling plates, with 96 samples at a time
- Even sonication of all vessels- the device was able to deliver the same amount of sonication to all the wells, which was crucial for our application
- Support from Hielscher team- support was excellent, you were always there for us in training, to answer our questions and address problems, with suggestions that helped us get to our goal.
Thank you again for your help and assistance as we worked to optimize our protocols.”
Mark D. Driscoll, Ph.D. – Chief Scientific Officer – Intus Biosciences
UIP400MTP Microplate Sonicator for High-Throughput Sample Prep
Customers worldwide use the Hielscher UIP400MTP for the reliable, reproducible and convenient preparation of mass samples in multi-well plates. The UIP400MTP facilitates their daily work in biological, biochemical, life science, medical and clinical laboratories. With the intelligent software and temperature control of the Hielscher UIP400MTP, temperature is reliably controlled and heat-induced sample degradation avoided. Ultrasonic sample preparation with the Hielscher Ultrasonics UIP400MTP delivers highly reliable and reproducible results!
Request your free information package including applications and price for the UIP400MTP be filling the form below or contacting us via email or phone today!
Looking for an ultrasonic system to process higher volumes? The table below gives you an indication of the approximate processing capacity of our ultrasonicators:
The table below gives you an indication of the approximate processing capacity of our ultrasonic systems from compact hand-held homogenizers and High-throughput / MultiSample sonicators to industrial ultrasonic processors for commercial applications:
| Batch Volume | Flow Rate | Recommended Devices |
|---|---|---|
| 12-, 24,-, 48-, 96-, 384-, or 1536-well plates / microtiter plates, petri dishes, small vials | n.a. | UIP400MTP |
| 10 vials à 0.5 to 1.5mL | n.a. | VialTweeter at UP200St |
| 0.01 to 250mL | 5 to 100mL/min | UP50H |
| 0.01 to 500mL | 10 to 200mL/min | UP100H |
| 10 to 2000mL | 20 to 400mL/min | UP200Ht, UP400St |
| 0.1 to 20L | 0.2 to 4L/min | UIP2000hdT |
| 10 to 100L | 2 to 10L/min | UIP4000hdT |
| n.a. | 10 to 100L/min | UIP16000 |
| n.a. | larger | cluster of UIP16000 |
Plate sonicator UIP400MTP for any 96-well plates, microtiter plates and multi-well plates.
Multi-well plate sonicator UIP400MTP for reliable and efficient of any standard microplates.
Literature / References
- FactSheet UIP400MTP Plate-Sonicator for High-Throughput Sample Preparation – English version – Hielscher Ultrasonics
- FactSheet UIP400MTP Plate-Sonicator für die High-Throughput Probenvorbereitung in 96-Well-Platten – deutsch – Hielscher Ultrasonics
- InfoGraphic UIP400MTP Sistema ultrasonidos para placa multipocillos – espanol/spanish – Hielscher Ultrasonics
- FactSheet Cool350 Lab chiller for UIP400MTP – Hielscher Ultrasonics
- Dreyer J., Ricci G., van den Berg J., Bhardwaj V., Funk J., Armstrong C., van Batenburg V., Sine C., VanInsberghe M.A., Marsman R., Mandemaker I.K., di Sanzo S., Costantini J., Manzo S.G., Biran A., Burny C., Völker-Albert M., Groth A., Spencer S.L., van Oudenaarden A., Mattiroli F. (2024): Acute multi-level response to defective de novo chromatin assembly in S-phase. Molecular Cell 2024.
- Mochizuki, Chika; Taketomi, Yoshitaka; Irie, Atsushi; Kano, Kuniyuki; Nagasaki, Yuki; Miki, Yoshimi; Ono, Takashi; Nishito, Yasumasa; Nakajima, Takahiro; Tomabechi, Yuri; Hanada, Kazuharu; Shirouzu, Mikako; Watanabe, Takashi; Hata, Kousuke; Izumi, Yoshihiro; Bamba, Takeshi; Chun, Jerold; Kudo, Kai; Kotani, Ai; Murakami, Makoto (2024): Secreted phospholipase PLA2G12A-driven lysophospholipid signaling via lipolytic modification of extracellular vesicles facilitates pathogenic Th17 differentiation. BioRxiv 2024.
- Chen J.-X; Cipriani P.G.; Mecenas D.; Polanowska J.; Piano F.; Gunsalus K.C.; Selbach M. (2016): In Vivo Interaction Proteomics in Caenorhabditis elegans Embryos Provides New Insights into P Granule Dynamics. Molecular & Cellular Proteomics 15.5; 2016. 1642-1657.
- LeThanh, H.; Neubauer, P.; Hoffmann, F. (2005): The small heat-shock proteins IbpA and IbpB reduce the stress load of recombinant Escherichia coli and delay degradation of inclusion bodies. Microb Cell Fact 4, 6; 2005.
- Martínez-Gómez A.I.; Martínez-Rodríguez S.; Clemente-Jiménez J.M.; Pozo-Dengra J.; Rodríguez-Vico F.; Las Heras-Vázquez F.J. (2007): Recombinant polycistronic structure of hydantoinase process genes in Escherichia coli for the production of optically pure D-amino acids. Appl Environ Microbiol. 73(5); 2007. 1525-1531.
Facts Worth Knowing
What are Microtiter Plates?
Microtiter plates, also known as 96-well plates, 96-well microplates, multi-well plates or ELISA plates, are rectangular multi-well plates, which are widely used in laboratories to process cultured cells, cellular samples. Microtiter plates are commonly used in manifold assays such as ELISA and PCR. Most 96-well plates are designed for high-throuput workflows and are compatible with analytical instruments and robotic handling and processing. Therefore multiple variants such as fully skirted plates suitable for robotic platforms, non-skirted, semi-skirted, and bar-coded plates adapted to mass sample processing are available.
Common use of microtiter plates include applications such as sample collection, compound preparation, combinatorial chemistry, high throughput screening, nucleic acid purification, bacterial culture growth, and plate replication.
The UIP400MTP is highly efficient for the rapid sample preparation for high throughput screening.