High-Throughput Sample Preparation for Biomarker Diagnostics: Ultrasonic Protein Extraction

The integration of high-throughput sonicators in biomarker diagnostics offers substantial advantages, enhancing the efficiency, consistency, and scalability of sample preparation processes. By enabling the rapid and uniform extraction of biomolecules from diverse sample types, the Multiwell-Plate Sonicator UIP400MTP supports the high demands of modern biomarker research and diagnostic, ultimately advancing the understanding of diseases and improving therapeutic strategies. Learn how the Multiwell-Plate Sonicator UIP400MTP streamlines the isolation of proteins from cells, tissues, blood and other sample fluids.

Proteins as Biomarkers in Diagnostic

Biomarker diagnostics play a critical role in both clinical and research settings. Biomarkers are measurable indicators of biological states or conditions and can include proteins, nucleic acids, lipids, small molecules, or other types of molecules.

High-Throughput Assays

96-well plate sonicator UIP400MTP for high-throughput processing of large sample numbers in microtiter and multi-well plates in biomarker diagnostics (e.g. assays, PCR, ELISA)Advances in proteomics have enabled the simultaneous measurement of multiple protein biomarkers, enhancing diagnostic accuracy and providing a comprehensive overview of a patient’s health status. Ultrasonic sample preparation with the Multiwell-Plate Sonicator UIP400MTP combined with techniques such as mass spectrometry and protein microarrays allow for high-throughput analysis, facilitating the identification of biomarker panels that can improve diagnostic precision.
The stability and reproducibility of protein measurements are crucial for reliable diagnostics. Sample preparation such as a gentle, yet effective extraction of proteins from samples as well as rigorous validation and standardization of protein biomarker assays are essential to ensure clinical utility.
The high-throughput sonicator UIP400MPT significantly enhances protein extraction and biomarker analysis, providing researchers with powerful tools to advance their studies and achieve robust, reproducible results.
Facilitating the throughput of high sample numbers and providing reliable, meaningful results, the Multiwell-Plate Sonicator UIP400MTP enhances the full potential of protein biomarkers in diagnostics, marking a significant stride in the field of precision medicine.

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UIP400MTP Plate Sonicator for high-throughput sample preparation: The UIP400MTP uniformly sonicates samples in multi-well, microtiter plates and 96-well plates disrupting cells, extracting proteins, fragmenting DNA, RNA and alpha-synuclein fibrils.

UIP400MTP Plate Sonicator for high-throughput sample preparation uniformly sonicates samples in multi-well and 96-well plates

Enhanced Protein Extraction with the Multi-well Plate Sonicator UIP400MTP

The high-throughput sonicator UIP400MTP offers significant advantages for protein extraction and biomarker analysis.

  • High Protein Yields: Protein extraction in high-throughput using your standard multi-well plate of choice … With the UIP400MPT, extract proteins from tissues, blood, plasma, urine, saliva and cell suspensions to analyze the downstream effects of genetic coding and protein-protein interactions. This facilitates the analysis of biomarkers and allows to explore their effects.
  • Rapid Identification: Identify proteomic biomarkers and select therapeutic targets for improved drug treatments. The UIP400MPT facilitates the identification of proteomic biomarkers, enabling the selection of therapeutic targets for improved drug treatments.
  • Large Sample Throughput: Using any standard multi-well of microtiter plate enable you to sonicate 1 to 1536 samples simultaneously.With 400 watts of power and precise control over sonication intensity, the UIP400MPT can ultrasonicate samples within minutes. High sample number ensure high confidence results – obtaining accurate, reproducible data and subsequently meaningful assay results.
  • Efficiency: The UIP400MPT features high power and precise control enable efficient and uniform protein extraction, crucial for reliable biomarker analysis.
  • Scalability: The ability to process multiwell plates with up to 1536 samples simultaneously makes it ideal for large-scale studies and high-throughput screening.
  • Versatility: Suitable for a wide range of sample types including cells, tissues, blood plasma, saliva, and urine, the UIP400MPT supports diverse research needs.
  • Speed: Rapid sample processing accelerates workflows and data acquisition, enhancing overall research productivity.
UIP400MTP Multiwell Plate Sonicator for high-throughput sample preparation in biomarker diagnostic.

UIP400MTP Multiwell Plate Sonicator for high-throughput sample preparation in biomarker diagnostic.


Ultrasonic Sample Preparation for Biomarker Diagnostics

With the UIP400MTP, the high-throughput extraction of proteins, DNA and other molecules from various sample types for biomarker diagnostics becomes facile and efficient. The UIP400MTP enables you to utilize a range of biological samples, including the following:

Cell SuspensionsFrom cultured cells or clinical samples for detailed molecular studies.
Tissue BiopsiesProvide localized information from specific organs or tumors.
Blood and PlasmaCommonly used due to their accessibility and the wealth of information they can provide about systemic conditions.
SalivaNon-invasive and increasingly used for various diagnostics.
Cerebrospinal Fluid (CSF)Used for neurological conditions due to its proximity to the brain.
UrineNon-invasive and useful for monitoring metabolic and renal functions.
Advantages of the multi-well plate sonicator for high-throughput sample preparation in biomarker diagnostics.

The multi-well plate sonicator UIP400MTP offers numerous additional benefits.


The video shows the ultrasonic sample preparation system UIP400MTP, which allows for the reliable sample preparation of any standard multi-well plates using high-intensity ultrasound. Typical applications of the UIP400MTP include cell lysis, DNA, RNA, and chromatin shearing as well as protein extraction.

Ultrasonicator UIP400MTP for multi-well plate sonication

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Design, Manufacturing and Consulting – Quality Made in Germany

Hielscher ultrasonicators are well-known for their highest quality and design standards. Robustness and easy operation allow the smooth integration of our ultrasonicators into industrial facilities. Rough conditions and demanding environments are easily handled by Hielscher ultrasonicators.

Hielscher Ultrasonics is an ISO certified company and put special emphasis on high-performance ultrasonicators featuring state-of-the-art technology and user-friendliness. Of course, Hielscher ultrasonicators are CE compliant and meet the requirements of UL, CSA and RoHs.

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Literature / References

Facts Worth Knowing

What Methods are Applied for Biomarker Diagnostics?

Biomarker diagnostics are crucial in the early detection, diagnosis, and management of various diseases. There are several methods used in biomarker diagnostics, which can be broadly categorized based on the type of biomarker (e.g., genetic, protein, metabolic) and the techniques employed. Below are some common methods listed:

  • Molecular Techniques
    Polymerase Chain Reaction (PCR): Amplifies specific DNA sequences to detect genetic mutations, pathogens, or specific biomarkers.
    Quantitative PCR (qPCR): Measures the quantity of DNA or RNA in a sample, providing information on gene expression levels.
    Digital PCR: Offers highly precise quantification of DNA or RNA molecules.
  • Immunoassays
    Enzyme-Linked Immunosorbent Assay (ELISA): Detects and quantifies proteins, hormones, and antibodies in a sample.
    Western Blotting: Identifies specific proteins in a sample by separating them via gel electrophoresis and detecting with antibodies.
    Flow Cytometry: Analyzes the physical and chemical characteristics of cells or particles using fluorescently labeled antibodies.
  • Mass Spectrometry
    Matrix-Assisted Laser Desorption/Ionization (MALDI): Analyzes large biomolecules by ionizing them and measuring their mass-to-charge ratio.
    Liquid Chromatography-Mass Spectrometry (LC-MS): Combines liquid chromatography with mass spectrometry for detailed molecular analysis.
  • Next-Generation Sequencing (NGS)
    Whole Genome Sequencing (WGS): Provides a comprehensive view of the entire genome.
    Whole Exome Sequencing (WES): Focuses on the protein-coding regions of the genome.
    RNA Sequencing (RNA-Seq): Analyzes the entire transcriptome to measure gene expression and identify RNA biomarkers.
  • Proteomics
    Two-Dimensional Gel Electrophoresis (2D-GE): Separates proteins based on their isoelectric point and molecular weight.
    Protein Microarrays: Simultaneously detect and quantify multiple proteins in a sample.
  • Metabolomics
    Nuclear Magnetic Resonance (NMR) Spectroscopy: Identifies and quantifies metabolites in biological samples.
    Gas Chromatography-Mass Spectrometry (GC-MS): Analyzes volatile and semi-volatile compounds.
  • Cytogenetics
    Fluorescence In Situ Hybridization (FISH): Detects and localizes the presence or absence of specific DNA sequences on chromosomes.
    Comparative Genomic Hybridization (CGH): Detects chromosomal abnormalities and variations.
  • Bioinformatics and Data Analysis
    Machine Learning Algorithms: Analyze large datasets to identify potential biomarkers and patterns.
    Statistical Analysis: Ensures the reliability and significance of biomarker data.
  • Point-of-Care Testing
    Lateral Flow Assays: Used for rapid, on-site diagnostics (e.g., pregnancy tests, rapid COVID-19 tests).
    Biosensors: Devices that detect specific biological molecules and provide real-time results.

These methods and techniques often complement each other, providing a comprehensive approach to biomarker discovery and diagnostics. The choice of method depends on the specific biomarker, the disease context, and the required sensitivity and specificity.

High performance ultrasonics! The Hielscher product range covers the full spectrum from the compact lab ultrasonicator over bench-top units to full-industrial ultrasonic systems.

Hielscher Ultrasonics manufactures high-performance ultrasonic homogenizers from lab to industrial size.

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