Non-Contact Sonicators
Ultrasonicators play a critical role in the extraction and processing of biological samples for genomics, proteomics, and diagnostic applications. By effectively disrupting a wide range of cell and tissue types, ultrasonicators facilitate the isolation and analysis of DNA, RNA, and proteins, thereby advancing research in molecular biology and biotechnology. Whether working with bacterial cells or human tissues, researchers rely on the precision and efficiency of ultrasonicators to obtain high-quality biological extracts for their studies.
Hielscher Ultrasonics supplies powerful non-contact sonicators for sample preparation and clinical analysis. The Multi-well plate sonicator UIP400MTP, the VialTweeter, the CupHorn and the GDmini2 flow sonicator process the samples without touching them.
High-throughput sonicator UIP400MTP for lysis, protein and DNA / RNA purification and nucleic acid shearing.
High-Throughput Sonicators for Lysis and DNA Shearing
For the processing of high sample sample numbers, Hielscher Ultrasonics offers state-of-the-art non-contact ultrasonicators, which allow the simultaneous sonication of numerous samples in 96-well, multi-well and microtiter plates, test tubes and vials or small vessels.
Depending on your sample number and your preferred sample container, you can choose between the Multi-well Plate Sonicator UIP400MTP, the VialTweeter or the CupHorn. If you want to sonicate smaller volume streams inline, the GDmini2 inline reactor is the ideal ultrasonic setup for you.
A major advantage of all Hielscher multi-sample sonicators is the fact that you can use the sample container of your choice! No need to buy expensive proprietary plates or tubes! Select the usual standard multi-well plates and test vials that are ideal for your experiments.
Read more about Hielscher non-contact sonicators for sample preparation!
The multi-well plate sonicator UIP400MTP offers numerous additional benefits.
Benefits of High-Throughput, Non-Contact Sonicators in Genomics, Proteomics, Biomarker Diagnostics and Life Science
Non-contact sonicators are powerful tools for high-throughput or multi-sample preparation in biomarker analysis and life science.
| Efficient Cell Lysis and Tissue Disruption | Hielscher high-throughput, non-contact sonicators effectively lyse cell suspensions and tissues, ensuring comprehensive release of intracellular components, which is critical for accurate biomarker analysis. |
| Scalability and Throughput | By accommodating 96-well and multiwell plates or multiple test tubes, high-throughput sonicators enable the processing of numerous samples simultaneously. This scalability is essential for large-scale studies and high-throughput screening applications. |
| Uniform Sample Processing | Ensuring consistency across multiple samples is crucial for reliable biomarker quantification. Sonication provides uniform lysis conditions, reducing variability between samples. |
| Non-Contact Sonication | With Hielscher non-contact sonicators, you can process high sample number in sealed containers without adding or inserting anything into the sample. This provides any cross-contamination and sample loss. |
| Versatile Applications | Multi-sample sonicators can extract a wide range of biomolecules, including proteins, DNA, RNA, and metabolites, from various sample types. Nucleic acid shearing is another power application of ultrasonicators. By adjusting sonication intensity, DNA and RNA can be fragmented to a target basepair length. This versatility makes them indispensable in life science, genomic and proteomic studies as well as for diagnostic screenings. |
| Reduced Processing Time | Whether you are looking for multi-sample or high-throughput sonication, Hielscher has the suitable non-contact sonicator for your life science application. The ability to process many samples in parallel significantly reduces the time required for sample preparation, facilitating faster experimental workflows and data acquisition. |
Multi-Sample Sonicator “VialTweeter” for the simultaneous sample preparation of multiple sealed vials and test tubes
Join the community of scientists and industry leaders who trust Hielscher Ultrasonics to deliver cutting-edge ultrasonic solutions that drive progress and innovation in life sciences. For more information on selecting the right ultrasonicator and exploring its applications in life science, feel free to contact our team of experts. We are here to help you achieve and facilitate your research goals with the best ultrasonication solutions. Whether you are looking for high-throughput sonication or a customized solution, we have the right sonicator for your life science experiments.
The table below gives you an indication of the approximate processing capacity of our lab-size ultrasonicators used in life science, genomics, proteomics and diagnostics:
| Recommended Devices | Batch Volume | Flow Rate |
|---|---|---|
| UIP400MTP 96-Well Plate Sonicator | multi-well / microtiter plates | n.a. |
| Ultrasonic CupHorn | CupHorn for vials or beaker | n.a. |
| GDmini2 | ultrasonic micro-flow reactor | n.a. |
| VialTweeter | 0.5 to 1.5mL | n.a. |
| UP100H | 1 to 500mL | 10 to 200mL/min |
| UP200Ht, UP200St | 10 to 1000mL | 20 to 200mL/min |
| UP400St | 10 to 2000mL | 20 to 400mL/min |
| Ultrasonic Sieve Shaker | n.a. | n.a. |
Contact Us! / Ask Us!
Plate sonicator UIP400MTP for any 96-well plates, microtiter plates and multi-well plates.
Literature / References
- FactSheet UIP400MTP Plate-Sonicator for High-Throughput Sample Preparation – English version – Hielscher Ultrasonics
- FactSheet VialTweeter – Sonicator for Simultaneous Sample Preparation
- FactSheet UIP400MTP Plate-Sonicator für die High-Throughput Probenvorbereitung in 96-Well-Platten – deutsch – Hielscher Ultrasonics
- Jorge S., Pereira K., López-Fernández H., LaFramboise W., Dhir R., Fernández-Lodeiro J., Lodeiro C., Santos H.M., Capelo-Martínez J.L. (2020): Ultrasonic-assisted extraction and digestion of proteins from solid biopsies followed by peptide sequential extraction hyphenated to MALDI-based profiling holds the promise of distinguishing renal oncocytoma from chromophobe renal cell carcinoma. Talanta, 2020.
- Nordenfelt P, Waldemarson S, Linder A, Mörgelin M, Karlsson C, Malmström J, Björck L. (2012): Antibody orientation at bacterial surfaces is related to invasive infection. Journal of Experimental Medicine 17;209(13), 2012. 2367-81.
- Giricz Z., Varga Z.V., Koncsos G., Nagy C.T., Görbe A., Mentzer R.M. Jr, Gottlieb R.A., Ferdinandy P. (2017): Autophagosome formation is required for cardioprotection by chloramphenicol. Life Science Oct 2017. 11-16.
Frequently Asked Questions
What Differentiates Non-Contact Sonication from Direct Sonication?
Non-contact sonication and direct sonication are two techniques used in various scientific and industrial applications to apply ultrasonic energy to samples. The primary difference between these methods lies in how the ultrasonic energy is delivered to the sample.
Non-contact sonication, also known as indirect sonication, involves transmitting ultrasonic energy to a sample without the sonicator probe coming into direct contact with the sample. This is typically achieved using a water coupling agent or a specialized holder where the sample is placed, and the ultrasonic energy is transmitted through the liquid medium (usually water) and the sample container wall into the sample. Non-contact sonication is commonly employed in biological, chemical and life science laboratories for tasks such as cell disruption, cell solubilization, protein extraction, DNA shearing and pre-analytical sample prep, especially for samples sensitive to contamination. The primary advantages include minimizing contamination risks, reducing the potential for sample overheating, and allowing for the simultaneous processing of multiple samples.
Direct sonication, on the other hand, involves the ultrasonic probe (also called a horn or sonotrode) being immersed directly into the sample. This method allows for a more efficient and direct transfer of ultrasonic energy to the sample. Since the probe is directly immersed in the sample, the energy transfer is very efficient, making it ideal for applications requiring intense ultrasonic energy. Direct sonication is commonly used in cell disruption, homogenization, and sonochemical reactions, particularly in processes requiring high-intensity sonication, such as nanoparticle synthesis. The advantages of direct sonication include more efficient and faster processing due to direct energy transfer and suitability for high-intensity applications.
Hielscher Ultrasonics manufactures high-performance ultrasonic homogenizers from lab to industrial size.