Ultrasonic topic: "Ultrasonic Lysis and Cell Disruption"
Lysis is the term which describes the disruption of cell walls or membranes. Ultrasonic disruptors are a reliable tool to break cells in order to release intracellular compounds such as DNA, proteins, organelles, and phytochemicals. For ultrasonic lysis (aka cell disruption), high-intensity / low-frequency ultrasound waves are applied to disrupt the cell membranes. Since ultrasonication promotes mass transfer, the intracellular material is released into the surrounding solvent. The fluid containing the contents of lysed cells is called a lysate.
Cell lysis is the first step in cell fractionation, organelle isolation and protein extraction and purification.
Read more about ultrasonic cell disruptors and ultrasonic lysis!
Found 56 hits. Showing results 37 - 48.
Ultrasonic Stem Cell Isolation
Sonication is a highly efficient mechanical method for isolating stem cells from human adipose tissue. The ultrasonically isolated stromal vascular fraction (SVF) demonstrates significant regenerative potential for medical applications. Hielscher Ultrasonics provides a range of direct and indirect sonication options…
https://www.hielscher.com/ultrasonic-stem-cell-isolation.htm
EPA3550 Ultrasonic Extraction Guide
Ultrasonic extraction is a green, environmental-friendly method of extraction that can be applied to small lab samples as well as for the extraction of valuable compounds on commercial production scale. The United State Environmental Protection Agency (EPA) recommends a variety…
https://www.hielscher.com/epa3550-ultrasonic-extraction-guide.htm
Power-Ultrasound for Advanced Microbial Stability of Paints
Microbial deterioration can damage both water- and solvent-borne paint formulations. Environmental regulations restrict the use of biocides. Sonication is an environmental-friendly, effective method of paint preservation. Power ultrasound allows even for the recycling of microbially spoilt paints and coatings. Ultrasonic…
https://www.hielscher.com/power-ultrasound-for-advanced-microbial-stability-of-paints.htm
Ultrasonic Ballast Water Disinfection
The ultrasonic treatment system has demonstrated to be effective in the killing of bacteria, plankton, and larger organisms. The ultrasonic disinfection of ballast water is a mechanical/ physical treatment, which avoids the dosing of any strong and expensive active chemicals…
https://www.hielscher.com/ultrasonic-ballast-water-disinfection.htm
Ultrasonic Extraction of Cellular Matter
Ultrasonic extraction or sono-extraction is a process intensifying technology, which works by the coupling of high power ultrasonics into a slurry of plant or cell tissue. Hielscher Ultrasonics supplies reliable ultrasonicators for cell disruption and extraction from small lab samples…
https://www.hielscher.com/ultrasonic-extraction-of-cellular-matter.htm
Closed Ultrasonic Reactor for Pharmaceutical Batch Processing
Many processes as mixing, dispersing and extraction of high-value materials are carried out as batch process. Hielscher's closed ultrasonic reactor is ideal for an integrated ultrasonic processing under closed and precisely controlled conditions. Hielscher's closed ultrasonic reactor is perfectly suitable…
https://www.hielscher.com/closed-ultrasonic-reactor-for-pharmaceutical-batch-processing.htm
Ultrasonic Tissue Homogenizers
Ultrasonication is a safe & efficient method of tissue homogenization. Ultrasonic applications include the preparation tissue and suspensions, the extraction of proteins, DNA/ RNA and active compounds, as well as the activation / inactivation of enzymes and yeasts. The precise…
https://www.hielscher.com/ultrasonic-tissue-homogenizers.htm
How To Use Hielscher Ultrasonic Tissue Homogenizers
Before purifying or characterizing intracellular macromolecules such as proteins, organelles, enzymes, or active compounds, it is essential to employ an efficient method for tissue lysis and cell disintegration. Ultrasonication is a highly effective technique for cell preparation, quickly releasing intracellular…
https://www.hielscher.com/how-to-use-hielschers-ultrasonic-tissue-homogenizers.htm
UP200St with VialTweeter for Simultaneous Sample Preparation
In laboratories, it is often necessary to prepare multiple samples simultaneously at the same process conditions. The VialTweeter enables for the simultaneous sonication of up to 10 vials at the same intensity. Thereby, the VialTweeter is a reliable lab homogenizer…
https://www.hielscher.com/up200st-with-vialtweeter-for-simultaneous-sample-preparation.htm
Sonication for Cell Lysis: Cell Disruption and Extraction
Ultrasonic cell lysis is a widely applied sample‑preparation technique in modern biotech laboratories. Its primary purpose is to disrupt cellular membranes or entire cells in order to release intracellular components such as proteins, nucleic acids, or organelles. In daily lab…
https://www.hielscher.com/ultrasonic-lysis-cell-disruption-extraction.htm
Algae Grow Lab – Ultrasonic Algae Extraction
Algae Cultivation Algae Grow Lab developed a series of tubular and flat photobioreactors for algae cultivation as well as a process of cell ultrasonic destruction based on Hielscher ultrasonic processors equipped with flow cells. The general flow diagram of the…
https://www.hielscher.com/algae-grow-lab.htm
UIP500hdT – Industrial Processing in Small Scale
The ultrasonic processor UIP500hdT (20kHz, 500W) is an industrial grade device for pilot testing and small scale processing of liquids. Amongst the most common applications of the UIP500hdT are homogenization, dispersing, emulsification, cell disintegration and sonochemical reactions. A full color…
https://www.hielscher.com/i500_p.htm