Ultrasonic topic: "Ultrasonic Lysis and Cell Disruption"
Lysis is the term which describes the disruption of cell walls or membranes. Ultrasonic disruptors are a reliable tool to break cells in order to release intracellular compounds such as DNA, proteins, organelles, and phytochemicals. For ultrasonic lysis (aka cell disruption), high-intensity / low-frequency ultrasound waves are applied to disrupt the cell membranes. Since ultrasonication promotes mass transfer, the intracellular material is released into the surrounding solvent. The fluid containing the contents of lysed cells is called a lysate.
Cell lysis is the first step in cell fractionation, organelle isolation and protein extraction and purification.
Read more about ultrasonic cell disruptors and ultrasonic lysis!
Found 58 hits. Showing results 49 - 58.
Algae Grow Lab – Ultrasonic Algae Extraction
Algae Cultivation Algae Grow Lab developed a series of tubular and flat photobioreactors for algae cultivation as well as a process of cell ultrasonic destruction based on Hielscher ultrasonic processors equipped with flow cells. The general flow diagram of the…
https://www.hielscher.com/algae-grow-lab.htm
Sonicator UIP1000hdT (1000 Watts) – Powerful and Versatile
The UIP1000hdT is a 1,000-watt industrial sonicator for pilot-scale process development, small production, and continuous liquid processing. It is designed for demanding ultrasonic applications such as homogenization, emulsification, dispersion, particle size reduction, extraction, cell lysis, dissolving, and sonochemical reactions. With…
https://www.hielscher.com/i1000_p.htm
Ultrasonic Processor UIP2000hd – High-Efficiency
The ultrasonic processor UIP2000hd (20kHz, 2000W) is a powerful and adaptable ultrasonic device for the industrial processing of liquids. It is used for applications, such as emulsification, dispersing & particle fine milling, lysis & extraction or homogenizing. Despite the enormous…
https://www.hielscher.com/i2000_p.htm
UIP500hdT – Industrial 500W Sonicator
The UIP500hdT is a 500W industrial sonicator for pilot-scale research, process development, and continuous small-scale manufacturing. It is widely used for ultrasonic homogenization, emulsification, dispersion, extraction, cell lysis, and sonochemical reactions in the chemical, pharmaceutical, food, biotechnology, and nanomaterials industries.…
https://www.hielscher.com/i500_p.htm
Ultrasonic Homogenizers for Lab, Pilot and Industrial Processing
Ultrasonic homogenizers are powerful tools to mix and homogenize solid-liquid and liquid-liquid suspensions. Ultrasonic homogenization is a mechanical process to reduce particles in a liquid so that they become uniformly small and evenly distributed. Hielscher offers powerful ultrasonic homogenizers for…
https://www.hielscher.com/homogenize_01.htm
Ultrasonic Extraction and Preservation
Ultrasonic extraction and preservation utilizes using power ultrasound for the disintegration of cell structures (lysis). Breaking cells with ultrasonics results in highly efficient extraction of intra-cellular compounds as well as microbial inactivation. Due to numerous advantages, ultrasonication is widely used…
https://www.hielscher.com/extraction_01.htm
UP400S – Powerful Sonication of Larger Samples
400 Watts ultrasonic power - The UP400S (400W, 24kHz) is a powerful and reliable ultrasonic device for the sonication of larger samples in the lab. Typical application include: Homogenization, deagglomeration, lysis and cell disintegration, protein extraction and the emulsification of…
https://www.hielscher.com/400s_p.htm
UP50H – Compact Lab Homogenizer
The ultrasonic processor UP50H is typically used for the sonication of small lab samples. This homogenizer allows for the handheld or stand-mounted emulsification, dispersing, dissolving or cell disruption. The ultrasonic processor UP50H (50 watts, 30kHz) is the smallest model of…
https://www.hielscher.com/50h_p.htm
Ultrasonic Disintegration of Cells
Ultrasonication is an effective means of disintegrating cell structures. Therefore, sonicators are widely used in laboratories to break open cells, extract intracellular molecules, proteins and organelles for research and analysis. On industrial scale, ultrasonic disintegration and lysis is used to…
https://www.hielscher.com/cell_disintegration_01.htm
UP100H – Compact Ultrasonic Laboratory Device
The ultrasonic processor UP100H (100W, 30kHz) is the perfect device for the sonication of small and medium size lab samples. This compact, yet powerful, lab homogenizer is commonly used for sample preparation, such as emulsifying, dispersing, dissolving and cell disruption.…
https://www.hielscher.com/100h_p.htm