Hielscher Ultrasound Technology

UP400S – Powerful Sonication of Larger Samples

400 Watts ultrasonic power – The UP400S (400W, 24kHz) is a powerful and reliable ultrasonic device for the sonication of larger samples in the lab. Typical application include: Homogenization, deagglomeration, lysis and cell disintegration, protein extraction and the emulsification of liquids.

(Click for larger view!) Ultrasonic homogenizer UP400S with sonotrode H22.The ultrasonic processor UP400S (400 watts, 24kHz) is our most powerful laboratory device. With sonotrodes of a diameter range from 3 to 40mm the device is suited for the sonication of sample volumes from 5 to 4000ml. In flow approx. 10 to 50 liters per hour can be sonicated. For the preparation of samples the UP400S is mainly used for bigger volumes. It is suited for the development of ultrasonic applications in the laboratory but also in the bench top optimization as well as for the production of small quantities. For production quantities a PC-control or a remote interface to a central control of the user’s facility is recommended in order to raise the process safety and reproducibility. The PC-control improves the reproducibilty when following specific sonication protocols. With special flow cells and flange connections liquids can also be sonicated at elevated temperatures and pressures.

This video shows the Hielscher ultrasonicator UP400S (400W) generating acoustic cavitation in water.

Ultrasonic Cavitation in Water using the UP400S

High power generates the intensive cavitation required, but this results in unwanted noise. For operating the UP400S we recommend to use the sound protection box.

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  • UP400S ultrasonic lab homogenizer for stand-mounted operation

    for stand-mounted operation, 400 watts, ultrasonic frequency 24kHz, automatic frequency tuning system, amplitude adjustable from 20 to 100%, pulse adjustable from 0 to 100%, dry running protected, with 9-pin DSUB interface, in portable case, for use with stand ST1-16 or sound protection box SB1-16, with mounting tools, IP40 grade, titanium horn with female thread M10x1 dimensions (LxWxH): 280x210x150mm, weight: 3.2kg



  • Ultrasonic horn, for intense sample sonication, 3mm diameter

    made of titanium, tip diameter 3mm, approx. length 100mm, male thread M10x1, for samples from 5ml up to 200ml



  • Ultrasonic horn, for intense sample sonication, 7mm diameter

    made of titanium, tip diameter 7mm, approx. length 100mm, male thread M10x1, for samples from 20ml up to 500mll



  • made of titanium, tip diameter 14mm, approx. length 100mm, male thread M10x1, for samples from 50ml up to 1000ml



  • Ultrasonic horn, for intense sample sonication, 22mm diameter

    made of titanium, tip diameter 22mm, approx. length 100mm, male thread M10x1, for samples from 100ml up to 2000ml



  • Ultrasonic horn, for intense reactor sonication, 22mm diameter

    made of titanium, tip diameter 22mm, approx. length 100mm, male thread M10x1, with seal for closed systems



  • Long ultrasonic horn, for intense reactor sonication, 22mm diameter

    made of titanium, tip diameter 22mm, approx. length 100mm, male thread M10x1, with seal for closed systems



  • Ultrasonic horn, for intense sample sonication, 40mm diameter

    made of titanium, tip diameter 40mm, approx. length 100mm, male thread M10x1, for samples from 200ml up to 4000ml



  • Lab clamp for sample sonication

    clamp for holding diam. 0 to 63mm, made of aluminum, for use with stand stands of up to 16.5mm, e.g. ST1-16



  • LabLift, e.g. for the intense sonication of liquids in glass beakers in the laboratory.

    for the easy positioning of samples under the ultrasonic probes to control immersion depth, stainless steel, footprint 100x100mm, adjustable height: 50 to 125mm



  • Timer to predefine sample sonication time

    from 00:00 to 99:59 (min:sec)



  • made of stainless steel, with cooling, hose connectors, for fluids, autoclavable, for operation with sonotrode H22D, volume approx. 15ml, with stand-adapter for stand ST1-16 (diameter 16mm)



  • made of glass, with cooling, hose connectors, for fluids, autoclavable, for operation with sonotrode H22D (or with sonotrode H22L2D in combination with flask adapter NSA3), volume approx. 250ml, with clamp for installation at stand, e.g. stand ST1-16 (diameter 16mm)



  • Flask Adapter NSA3

    for standard flask-neck, vertically adjustable, suitable for sonotrode H22L2D in combination with flow cell GD22K



  • Sound protection box for noise reduction of ultrasonic devices

    with vertically adjustable table and diameter 16mm stand bar, for use with devices UP200S or UP400S



  • Ultrasonication beaker for the indirect sonication of samples

    beaker for the indirect sonication of samples, with stand-adapter for stand ST1-16 (diameter 16mm)



  • Ultrasonication beaker for the indirect sonication of samples

    versatile, for display of current power, cumulated energy and cumulated operation time, 115V~1P, 50-60Hz



  • Power Meter

    versatile, for display of current power, cumulated energy and cumulated operation time, 230V~1P, 50-60Hz


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The UP400S (400W, 24kHz) is a powerful ultrasonic homogenizer.

Ultrasonicator UP400S with probe H22 in the sound enclosure SB1-16

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Dispersing Fumed Silicia in Water using the UP400S

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Ultrasonic Dispersion of Carbon Nanotubes: The Hielscher ultrasonicator UP400S (400W) disperses and detangles CNTs fast and efficiently into single nanotubes.

Dispersing of Carbon Nanotubes in Water using the UP400S

UP400S in transport case. (Click to enlarge!)

The ultrasonic device UP400S comes in a portable case.