UIP550MTP Microplate Sonicator
The most powerful ultrasonic solution for uniform sonication of 12-, 24-, 48-, 96-, and 384-well plates. Achieve consistent cell lysis, protein extraction, and DNA fragmentation across all wells simultaneously.
UIP550MTP – 550 W Ultrasonic Power for High-Throughput Sample Preparation
96-well plates, microplates, multi-well plates, microtiter plates or ELISA plates are used for the mass sample cultivation and processing of biological samples. With the microtiter plate ultrasonicator UIP550MTP, Hielscher offers a unique ultrasonic solution of the uniform and reliable sample preparation of 12-, 24,-, 48-, 96-, or 384-well plates. Common applications of the microtiter ultrasonicator UIP550MTP include cell lysis, protein extraction, cell homogenization and solubilization as well as DNA fragmentation.
- 550W Ultrasonic Power – the UIP550MTP processes demanding samples with ease
- Compatible with ANY standard multi-well plate
- Uniform sonication of each well
- Precisely adjustable amplitudes and advanced temperature control
- Fully automatable for high-throughput workflows
Tell us about your sample preparation task – Our sonication experts will recommend you tips for the most efficient ultrasonic sample prep!
Microplate Sonicator UIP550MTP
Who is the UIP550MTP Designed for?
For laboratories that already value the UIP400MTP, the UIP550MTP offers the next step: more ultrasonic capacity, more processing intensity, and more confidence when working with difficult samples, higher sample loads, or time-sensitive protocols.
Why Choose the UIP550MTP?
The UIP550MTP is built for buyers who need reliable, scalable, and reproducible microplate sonication. It enables simultaneous treatment of samples across the plate, reducing manual handling and eliminating the inconsistencies associated with probe-by-probe sonication. Since the samples remain in their plates or vessels, the UIP550MTP supports clean, efficient workflows with reduced risk of cross-contamination.
Use the UIP550MTP when you need a powerful microplate sonicator for high-throughput sample preparation, automated laboratory workflows, reproducible assay preparation, biological sample processing, and demanding research or analytical protocols.
Choose your own plate! The UIP550MTP works with any standard multi-well plate.
Advantages of the Hielscher Microplate Sonicator UIP550MTP
✅ High-Power, Focused Sonication: Achieving consistent and efficient sample disruption in multiwell plates can be difficult when using traditional methods like plate shakers or bead mills. These techniques often produce inconsistent lysate quality, which can negatively impact downstream applications. The Hielscher microplate sonicator UIP550MTP provides a solution by using high-intensity ultrasound waves to create cavitation, ensuring uniform disruption across all wells and resulting in reliable lysate preparation throughout the plate.
✅ High-Throughput: Scalability is another important factor in multiwell plate sample processing. Traditional equipment is often limited in handling multiple plates, making large-scale processing cumbersome. The UIP550MTP sonicator solves this issue by being compatible with standard plate formats (12-, 24-, 48-, 96-, or 384-wells). Its compact design allows for the easy addition of multiple units, enabling high-throughput sample preparation and simultaneous sonication of multiple plates.
✅ Sterile Sonication: Cross-contamination is a frequent concern with traditional methods, as sample splashing or carryover between wells can compromise data quality. The UIP550MTP minimizes this risk by indirectly sonicating the plates, removing the need for beads or chemicals. By covering the plates with foil or a lid, a closed system is created, preventing cross-contamination.
✅ Cost-Efficiency: Cost and flexibility are also key considerations. Many high-throughput sonicators require expensive proprietary plates, which limits options and increases costs. The UIP550MTP, however, works with any standard multiwell plate, allowing researchers to choose the most suitable and cost-effective plates for their experiments, reducing expenses on consumables.
✅ Streamlined Workflows: Traditional sample preparation techniques can be labor-intensive, requiring repetitive manual steps and long processing times. The UIP550MTP sonicator simplifies the workflow, reducing hands-on time and labor. It provides fast, simultaneous treatment of all wells, eliminating the need for multiple incubation steps or manual transfers. Specific sonication protocols can be saved and easily repeated, ensuring consistent and reproducible results.
✅ No Sample Loss: Sample recovery is often challenging with bead mills, as sample loss can occur during bead transfer. The UIP550MTP sonicator offers superior sample recovery and handling by eliminating the need for beads, reducing the complexity of separation steps and minimizing the risk of sample loss or contamination.
✅ Use Different Sample Vessels: The versatility of the UIP550MTP sonicator is another major advantage. It accommodates various plate formats and offers flexible sonication parameters, making it suitable for a wide range of sample types and applications. In addition to multiwell plates, it can be used for small vials, petri dishes, and other containers, for processes such as cell lysis, DNA fragmentation, emulsification, and degassing.
✅ Automation: Finally, the UIP550MTP sonicator integrates seamlessly with automated systems, allowing for smooth incorporation into auto-sampler lines. This compatibility ensures standardized, reproducible sample preparation, boosting efficiency and productivity in high-throughput environments.
Applications of the UIP550MTP High-Throughput Ultrasonicator
The UIP550MTP ultrasonicator fulfills numerous applications based on the type of sample. The following overview introduces you to the capabilities of the Hielscher UIP550MTP sonicator for sample preparation in biology, life science, genomics, proteomics and biomarker diagnostics.
| Application Category | Specific Applications |
|---|---|
| Cell Lysis |
|
| Tissue Processing |
|
| Nucleic Acid Extraction |
|
| Chromatin Shearing |
|
| Protein Extraction |
|
| Cell Culture Processing |
|
Typical Applications:
- NGS Library Prep
- Genomics
- FFPE Tissue Extraction
- Biomarker Diagnostics
- Antibody Elution
- Microbiome Research and Analysis
- Biofilm Dislodging
Microplate sonicator UIP550MTP
Precise Temperature Control During Microtiter Plate Sonication
The UIP550MTP is a powerful 550-watt ultrasonicator that transmits ultrasound waves and vibrations through the walls of a microtiter plate, indirectly affecting the samples. This process creates cavitation and shear forces within the medium. Like other mechanical cell disruption methods, sonication generates heat. However, unlike other techniques, the UIP550MTP offers advanced temperature monitoring and allows users to set temperature limits to prevent thermal degradation of biological samples.
The UIP550MTP is equipped with smart software that can be easily accessed through a digital touchscreen. This interface allows you to pre-set temperature limits and program the total energy input for your sample processing.
Monitoring Sample Temperature: The UIP550MTP features an intelligent software system and a pluggable temperature sensor. By connecting the sensor to the UIP550MTP and placing the sensor tip into one of the microtiter wells, you can specify a temperature range for your sonication process using the digital touchscreen menu. The device will automatically stop when the maximum temperature is reached and pause until the sample cools down to the lower end of the specified temperature range. At that point, sonication will resume automatically. This feature helps prevent heat-induced sample degradation.
To further delay reaching the critical temperature limit, you can pre-cool the 96-well plate, microtiter plate, or ELISA plate and its samples.
UIP550MTP: Automatic data logging on SD card
Multi-Well Plate Sonicator UIP550MTP for High-Throughput Sample Prep
Customers around the world rely on the Hielscher UIP550MTP for efficient, reliable, and reproducible sample preparation in multi-well plates. This device simplifies daily tasks in biological, biochemical, life science, medical, and clinical laboratories. With its intelligent software and advanced temperature control, the UIP550MTP ensures precise temperature management, preventing heat-induced sample degradation. Ultrasonic sample preparation with the UIP550MTP consistently delivers dependable and reproducible results.
Looking for an ultrasonic system to process larger volumes? The table below gives you an indication of the approximate processing capacity of Hielscher sonicators:
The table below lists our ultrasoniators from compact hand-held homogenizers and high-throughput / MultiSample sonicators to industrial ultrasonic processors for commercial liquid processing:
| Task | Recommended Sonicator |
|---|---|
| Small sample preparation | UP50H or UP100H |
| Routine lab homogenization | UP200Ht or UP200St |
| Larger lab batches / flow cell work | UP400St |
| Closed-vial sonication | VialTweeter or CupHorn |
| 96-well and multi-well plates | UIP400MTP / UIP550MTP |
| Micro-flow processing | GDmini2 |
“I am writing to thank you for our fantastic experience using your UIP400MTP sonicator for uniform and consistent bacterial lysis of fecal microbiome samples. We are pushing the envelope for high throughput lysis technology, and your instrument is well suited for the task. Some of the key highlights are:
- Ease of operation – instrument is easy to use, and customization is straightforward using the menu choices
- High throughput – excellent for handling plates, with 96 samples at a time
- Even sonication of all vessels- the device was able to deliver the same amount of sonication to all the wells, which was crucial for our application
- Support from Hielscher team – support was excellent, you were always there for us in training, to answer our questions and address problems, with suggestions that helped us get to our goal.
Thank you again for your help and assistance as we worked to optimize our protocols.”
Mark D. Driscoll, Ph.D. – Chief Scientific Officer – Intus Biosciences
Not only for multi-well plates, the UIP550MTP sonicator processes tube racks, too.
Sonicate Petri dishes with the UIP550MTP
Facts Worth Knowing: UIP550MTP Microplate Sonicator
What is the UIP550MTP?
The UIP550MTP is a high-power microplate sonicator for the ultrasonic processing of samples in microplates, PCR plates, multi-well plates, and other standardized lab vessels. It is the more powerful sibling of the Hielscher UIP400MTP and is designed for high-throughput sample preparation in research, diagnostics, biotechnology, pharmaceutical development, and analytical laboratories.
How is the UIP550MTP different from the UIP400MTP?
The UIP550MTP is positioned as the more powerful version of the UIP400MTP microplate sonicator. While both instruments are designed for uniform, non-contact sonication of multi-well plates, the UIP550MTP provides with 550 watts a higher ultrasonic power for more demanding samples, increased processing intensity, and workflows where faster or stronger sonication is required.
What applications is the UIP550MTP used for?
The UIP550MTP can be used for cell lysis, tissue extraction, protein extraction, DNA shearing, RNA shearing, chromatin fragmentation, sample homogenization, particle dispersion, nanoparticle processing, emulsification, degassing, extraction, and general high-throughput sample preparation in microplates.
Can the UIP550MTP sonicate a 96-well plate?
Yes. The UIP550MTP is designed for microplate and multi-well plate sonication, including 96-well plate workflows. It allows laboratories to process many samples simultaneously, helping to improve throughput, reproducibility, and workflow efficiency compared with individual probe sonication.
Is the UIP550MTP a non-contact sonicator?
Yes. The UIP550MTP is a non-contact microplate sonicator. Samples are processed inside the plate or vessel without an ultrasonic probe entering each well. This helps reduce sample loss, carryover, and cross-contamination while simplifying high-throughput sample handling.
Does the UIP550MTP require special plates or proprietary consumables?
The UIP550MTP is intended for workflows using standard microplates and common laboratory consumables. This makes it suitable for laboratories that want to keep using established plate formats, assay protocols, buffers, and sample preparation kits.
Why is uniform sonication important in microplates?
Uniform sonication is essential because every well should receive comparable ultrasonic treatment. Inconsistent energy distribution can lead to variable lysis efficiency, uneven extraction yields, inconsistent particle dispersion, and poor assay reproducibility. The UIP550MTP is designed to support reproducible ultrasonic processing across the plate.
Is the UIP550MTP suitable for automated laboratories?
Yes. The UIP550MTP is suitable for high-throughput environments and can support automated sample preparation workflows. Since samples remain in standardized plates, the system can fit into workflows using plate handling, liquid handling, and batch processing concepts.
Who should buy the UIP550MTP?
The UIP550MTP is ideal for laboratories that need more ultrasonic power than the UIP400MTP, process challenging biological or chemical samples, require reproducible high-throughput sonication, or want to reduce manual probe-based sample preparation. It is especially useful for biotech, pharmaceutical, diagnostic, academic, and analytical labs.
What are the main advantages of the UIP550MTP?
The main advantages of the UIP550MTP are higher ultrasonic power, non-contact sample processing, uniform treatment across multi-well plates, compatibility with standard lab consumables, reduced risk of cross-contamination, high-throughput operation, and reproducible results in demanding sample preparation workflows.
What are microtiter plates?
Microtiter plates, also known as 96-well plates, 96-well microplates, multi-well plates or ELISA plates, are rectangular multi-well plates, which are widely used in laboratories to process cultured cells, cellular samples. Microtiter plates are commonly used in manifold assays such as ELISA and PCR. Most 96-well plates are designed for high-throuput workflows and are compatible with analytical instruments and robotic handling and processing. Therefore multiple variants such as fully skirted plates suitable for robotic platforms, non-skirted, semi-skirted, and bar-coded plates adapted to mass sample processing are available.
Common use of microtiter plates include applications such as sample collection, compound preparation, combinatorial chemistry, high throughput screening, nucleic acid purification, bacterial culture growth, and plate replication.
What are ELISA plates?
ELISA plates are flat, multi-well plates typically made of polystyrene, designed for use in enzyme-linked immunosorbent assays (ELISA). These plates allow for the detection and quantification of proteins, antibodies, hormones, or other molecules by immobilizing the target antigen or antibody onto the plate surface. Through a series of binding, washing, and enzyme-substrate reactions, the presence and concentration of the target substance can be measured, often via colorimetric, fluorescent, or luminescent signals.
Can sonication damage cells?
Yes, sonication can damage cells, but the effect of cell disruption depends on the ultrasonic intensity. High-intensity ultrasound waves create cavitation and shear forces that can disrupt cell membranes. While this is beneficial for processes like cell lysis and extraction of intracellular materials, excessive sonication or improper temperature control can lead to unintended cell damage or degradation of sensitive biological samples. However, the Hielscher UIP550MTP multi-well plate sonicator is equipped with intelligent temperature monitoring and control features to minimize heat-induced damage and help ensure more controlled, reproducible cell disruption.
Literature / References
- FactSheet UIP550MTP Plate-Sonicator for High-Throughput Sample Preparation – English version – Hielscher Ultrasonics
- FactSheet UIP550MTP Microplate-Sonicator für die High-Throughput Probenvorbereitung in 96-Well-Platten – deutsch – Hielscher Ultrasonics
- Mochizuki, Chika; Taketomi, Yoshitaka; Irie, Atsushi; Kano, Kuniyuki; Nagasaki, Yuki; Miki, Yoshimi; Ono, Takashi; Nishito, Yasumasa; Nakajima, Takahiro; Tomabechi, Yuri; Hanada, Kazuharu; Shirouzu, Mikako; Watanabe, Takashi; Hata, Kousuke; Izumi, Yoshihiro; Bamba, Takeshi; Chun, Jerold; Kudo, Kai; Kotani, Ai; Murakami, Makoto (2024): Secreted phospholipase PLA2G12A-driven lysophospholipid signaling via lipolytic modification of extracellular vesicles facilitates pathogenic Th17 differentiation. BioRxiv 2024.
- Chen J.-X; Cipriani P.G.; Mecenas D.; Polanowska J.; Piano F.; Gunsalus K.C.; Selbach M. (2016): In Vivo Interaction Proteomics in Caenorhabditis elegans Embryos Provides New Insights into P Granule Dynamics. Molecular & Cellular Proteomics 15.5; 2016. 1642-1657.
- LeThanh, H.; Neubauer, P.; Hoffmann, F. (2005): The small heat-shock proteins IbpA and IbpB reduce the stress load of recombinant Escherichia coli and delay degradation of inclusion bodies. Microb Cell Fact 4, 6; 2005.
- Martínez-Gómez A.I.; Martínez-Rodríguez S.; Clemente-Jiménez J.M.; Pozo-Dengra J.; Rodríguez-Vico F.; Las Heras-Vázquez F.J. (2007): Recombinant polycistronic structure of hydantoinase process genes in Escherichia coli for the production of optically pure D-amino acids. Appl Environ Microbiol. 73(5); 2007. 1525-1531.
- Kotowska M.; Pawlik K.; Smulczyk-Krawczyszyn A.; Bartosz-Bechowski H.; Kuczek K. (2009): Type II Thioesterase ScoT, Associated with Streptomyces coelicolor A3(2) Modular Polyketide Synthase Cpk, Hydrolyzes Acyl Residues and Has a Preference for Propionate. Appl Environ Microbiol. 75(4); 2009. 887-896.
