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Utrasonic topic: "Ultrasonic Lysis and Cell Disruption"

Lysis is the term which describes the disruption of cell walls or membranes. Ultrasonic disruptors are a reliable tool to break cells in order to release intracellular compounds such as DNA, proteins, organelles, and phytochemicals. For ultrasonic lysis (aka cell disruption), high-intensity / low-frequency ultrasound waves are applied to disrupt the cell membranes. Since ultrasonication promotes mass transfer, the intracellular material is released into the surrounding solvent. The fluid containing the contents of lysed cells is called a lysate.
Cell lysis is the first step in cell fractionation, organelle isolation and protein extraction and purification.
Read more about ultrasonic cell disruptors and ultrasonic lysis!

Sonicators as the UP200St are used to prepare genetic material such as DNA, RNA, miRNA and the subsequent gene transfection via sonoporation. Sonoporation allows to transfect plant cells with genetic material using agrobacterium.

12 pages about this topic are being shown:

Sonicate any standard microplate and PCR plate with the UIP400MTP Multi-Well Plate Sonicator.

Protocol using Hielscher Multi-Sample Sonicators in Biofilm and Proteomic Analysis

The use of multi-sample sonicators, such as the VialTweeter Multi-Tube Sonicator and the UIP400MTP Microplate Sonicator, streamlines laboratory workflows for high-throughput applications. Both devices allow precise and uniform ultrasonic processing across multiple samples, minimizing variability and enhancing reproducibility. This is…

https://www.hielscher.com/protocol-using-hielscher-multi-sample-sonicators-in-biofilm-and-proteomic-analysis-2.htm

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