Utrasonic topic: "Ultrasonic Lysis and Cell Disruption"
Lysis is the term which describes the disruption of cell walls or membranes. Ultrasonic disruptors are a reliable tool to break cells in order to release intracellular compounds such as DNA, proteins, organelles, and phytochemicals. For ultrasonic lysis (aka cell disruption), high-intensity / low-frequency ultrasound waves are applied to disrupt the cell membranes. Since ultrasonication promotes mass transfer, the intracellular material is released into the surrounding solvent. The fluid containing the contents of lysed cells is called a lysate.
Cell lysis is the first step in cell fractionation, organelle isolation and protein extraction and purification.
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Bioengineered bacteria species such E. coli as well as genetically modified mammalian and plant cell types are used widely in biotech to express molecules. In order to release these synthesized bio-molecules, a reliable cell disruption technique is required. High-performance ultrasonication…https://www.hielscher.com/ultrasonic-lysis-of-bioengineered-cells-in-industrial-production.htm
Next Generation Sequencing (NGS) requires the reliable shearing and fragmentation of genomic DNA in order to sequence genomic DNA strands and to create genome libraries. The controlled fragmentation of DNA into DNA fragments is an essential sample preparation step required…https://www.hielscher.com/ultrasonic-dna-fragmentation-for-next-gen-sequencing.htm
BL21 cells are a strain of E. coli that is widely used in research laboratories, biotechnology and industrial production due to their capability to express proteins highly efficient. Ultrasonic cell disruption, lysis and protein extraction is the common method to…https://www.hielscher.com/cell-lysis-of-bl21-cells-by-ultrasonication.htm
Assays such as ELISA are widely used for in-vitro diagnostics, disease-related protein detection and quality control (e.g. monitoring food allergens). Ultrasonic sample preparation is a rapid, reliable and reproducible technique to lyse cell and isolate intracellular proteins, DNA, RNA and…https://www.hielscher.com/ultrasonic-sample-prep-for-elisa-assays.htm
The Hielscher VialTweeter is a unique ultrasonic multi-sample preparation unit, which is used to inactivate the coronavirus SARS-COV-2. The VialTweeter allow to prepare up to 10 sample vials simultaneously and is thereby the ideal unit for mass sample processing. Inactivation…https://www.hielscher.com/protocol-for-sars-cov-2-coronavirus-inactivation-with-sonication.htm
Sample preparation before analysis can require various pre-analytical processes such as tissue homogenization, lysis, extraction of proteins, DNA, RNA, organelles and other intracellular substances, dissolving and degassing. The VialTweeter is a unique ultrasonic device that prepares multiple sample tubes simultaneously…https://www.hielscher.com/sample-preparation-with-the-ultrasonic-vialtweeter.htm
The biosynthesis of human milk oligosaccharides (HMOs) via fermentation or enzymatic reactions is a complex, consuming and often low-yielding process. Ultrasonication increases the mass transfer between substrate and cell factories ans stimulates cell growth and metabolism. Thereby, sonication intensifies fermentation…https://www.hielscher.com/human-milk-oligosaccharides.htm
Ultrasonic dismembrators are commonly used in the laboratory, bench-top and industry to prepare homogeneous colloidal suspensions such as emulsions and dispersions with droplet or particle sizes in the submicron- and nano-range. Furthermore, ultrasonic dismembrators are used for cell disruption, lysis,…https://www.hielscher.com/most-efficient-ultrasonic-dismembrators.htm
Cell disintegration is a common task in biotechnology and in many applications that involve processing cells and intracellular material. Ultrasonic cell disintegrators are efficient and reliable, when it comes to breaking cell wall or membranes of plant cells, mammalian tissue,…https://www.hielscher.com/ultrasonic-disintegrators.htm
Ultrasonication is a powerful tool used in biology, molecular chemistry and biochemistry as well as in the production of pharmaceuticals. Bio-sciences use ultrasonic homogenizers to lyse cells and extract proteins and other intracellular materials, the pharma industry applied ultrasonics to…https://www.hielscher.com/coronavirus-covid-19-sars-cov-2-and-ultrasonics.htm
Ultrasonic lysis and extraction is a reliable and long-time established method for the disruption of cells and the subsequent release of viruses, viral proteins, DNA, and RNA. Ultrasonics in Coronavirus Research The extraction of viruses from organ tissue is an…https://www.hielscher.com/ultrasonics-virus-research.htm
Monk fruit (S. grosvenorii) extract and particularly its compound mogroside V is used as a natural non-caloric sweetener. Mogrosides. Ultrasonic extraction is a simple, rapid, reproducible, and reliable extraction method successfully applied to produce natural non-caloric sweetener from monk fruit.…https://www.hielscher.com/ultrasonic-cold-water-extraction-of-mogrosides-from-monk-fruit.htm