Utrasonic topic: "Ultrasonic Lysis and Cell Disruption"
Lysis is the term which describes the disruption of cell walls or membranes. Ultrasonic disruptors are a reliable tool to break cells in order to release intracellular compounds such as DNA, proteins, organelles, and phytochemicals. For ultrasonic lysis (aka cell disruption), high-intensity / low-frequency ultrasound waves are applied to disrupt the cell membranes. Since ultrasonication promotes mass transfer, the intracellular material is released into the surrounding solvent. The fluid containing the contents of lysed cells is called a lysate.
Cell lysis is the first step in cell fractionation, organelle isolation and protein extraction and purification.
Read more about ultrasonic cell disruptors and ultrasonic lysis!

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Video: Falcon Tube Sonicator
Experience how the Hielscher VialTweeter delivers powerful, contamination-free sonication inside a sealed 50 mL Falcon tube to accelerate workflows ranging from microbiology to formulation development. For more information about this Falcon tube sonicator, please click here!
https://www.hielscher.com/video-falcon-tube-sonicator.htm50 mL Falcon Tube Sonicator
This VialTweeter sonicator delivers up to 200 watts of focused sonication power to closed 50 mL Falcon tubes, centrifuge tubes or conical sample vials. The high amplitude and intense sonication enables rapid and uniform treatment in a closed Falcon tube. It ensures…
https://www.hielscher.com/50-ml-falcon-tube-sonicator.htmProtocol using Hielscher Multi-Sample Sonicators in Biofilm and Proteomic Analysis
The use of multi-sample sonicators, such as the VialTweeter Multi-Tube Sonicator and the UIP400MTP Microplate Sonicator, streamlines laboratory workflows for high-throughput applications. Both devices allow precise and uniform ultrasonic processing across multiple samples, minimizing variability and enhancing reproducibility. This is…
https://www.hielscher.com/protocol-using-hielscher-multi-sample-sonicators-in-biofilm-and-proteomic-analysis-2.htmProbe-Type Sonication for Sample Preparation: A Comprehensive Guide
Probe-type sonication is a powerful tool for disrupting cells, shearing DNA, and dispersing particles in liquid samples. Like all techniques in life science, microbiology and clinical analysis, sonication requires careful optimization to avoid sample damage, particularly when working with heat-sensitive…
https://www.hielscher.com/probe-type-sonication-for-sample-preparation-a-comprehensive-guide.htm96-Well Sonicators – Streamline High-Throughput Sample Preparation
The 96-well sonicator UIP400MTP is a high-power ultrasound device designed specifically for the effective sonication of multi-well plates. The UIP400MTP is designed to enable precise, uniform focused sonication across all wells in microtiter plates, widely used in laboratory settings for…
https://www.hielscher.com/96-well-sonicator.htmFocused Sonicators for High-Throughput Sample Processing
Focused sonicators are the solution for high-throughput sample preparation. The 96-Well Plate Sonicator UIP400MTP reliably processes samples in multi-well plates, 96-well plates, Petri dishes or test tubes and are specifically designed to meet the rigorous demands of laboratories and research…
https://www.hielscher.com/focused-sonicators-for-high-throughput-sample-processing.htmAssay Sonication – The Assay-at-Once Sonicator UIP400MTP
Sonication is an essential technique in laboratory protocols, particularly for applications like chromatin immunoprecipitation (ChIP) assays. By using ultrasonic energy to break down cells, shear DNA, and solubilize chromatin, sonication enables researchers to prepare samples more efficiently and reproducibly. The…
https://www.hielscher.com/assay-sonication-the-assay-at-once-sonicator-uip400mtp.htmSonication Devices for your Lab Tasks
Sonication devices, particularly probe-type sonicators, are advanced tools widely used in laboratory and industrial settings. Utilizing the power of ultrasound, sonication devices can process various materials effectively. These devices work by transmitting ultrasonic energy through a probe (or horn) into…
https://www.hielscher.com/sonication-devices-for-your-lab-tasks.htmMicrobiome Research and Clinical Analysis Streamlined by Sonication
Sonication is a powerful technique widely used in microbiome research and clinical analysis facilitating a high-throughput sample preparation. The ultrasonic method is particularly effective for processing complex biological samples, such as those containing microorganisms. The use of sonication facilitates cell…
https://www.hielscher.com/microbiome-reasearch-sonication.htmHigh-Throughput Sample Preparation for Mitochondrial Diagnostics
Mitochondrial diagnostics in research and clinics are performed using various techniques such as sequencing, PCR, and biochemical assays. These methods are used to identify DNA mutations and measure mitochondrial function. Sequencing helps detect genetic mutations, while PCR can quantify specific…
https://www.hielscher.com/mitochondrial-diagnostic.htmGenomic Research Facilitated by Sonication
Genomic research has revolutionized our understanding of biological systems, enabling the elucidation of complex genetic mechanisms underlying various diseases and traits. Sonication, a technique originally developed for the disruption of cellular membranes, has found extensive utility in genomic research. This…
https://www.hielscher.com/genomic-research-facilitated-by-sonication.htmUltrasonic Lysis: Step-by-Step Guide to Perfecting Cell Disruption
Do you want to master the science of cell lysis? Look no further! In this step-by-step guide, we will walk you through the process of ultrasonic cell disruption and ensure that your cell lysis technique gets you optimal results. Whether…
https://www.hielscher.com/ultrasonic-lysis-step-by-step-guide-to-perfecting-cell-disruption.htm