Utrasonic topic: "Ultrasonic Lysis and Cell Disruption"
Lysis is the term which describes the disruption of cell walls or membranes. Ultrasonic disruptors are a reliable tool to break cells in order to release intracellular compounds such as DNA, proteins, organelles, and phytochemicals. For ultrasonic lysis (aka cell disruption), high-intensity / low-frequency ultrasound waves are applied to disrupt the cell membranes. Since ultrasonication promotes mass transfer, the intracellular material is released into the surrounding solvent. The fluid containing the contents of lysed cells is called a lysate.
Cell lysis is the first step in cell fractionation, organelle isolation and protein extraction and purification.
Read more about ultrasonic cell disruptors and ultrasonic lysis!
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Ultrasonic DNA Fragmentation for Next Gen Sequencing
Next Generation Sequencing (NGS) requires the reliable shearing and fragmentation of genomic DNA in order to sequence genomic DNA strands and to create genome libraries. The controlled fragmentation of DNA into DNA fragments is an essential sample preparation step…
https://www.hielscher.com/ultrasonic-dna-fragmentation-for-next-gen-sequencing.htm
Cell Lysis of BL21 Cells by Ultrasonication
BL21 cells are a strain of E. coli that is widely used in research laboratories, biotechnology and industrial production due to their capability to express proteins highly efficient. Ultrasonic cell disruption, lysis and protein extraction is the common method…
https://www.hielscher.com/cell-lysis-of-bl21-cells-by-ultrasonication.htm
Ultrasonic Sample Prep for ELISA Assays
Assays such as ELISA are widely used for in-vitro diagnostics, disease-related protein detection and quality control (e.g. monitoring food allergens). Ultrasonic sample preparation is a rapid, reliable and reproducible technique to lyse cell and isolate intracellular proteins, DNA, RNA…
https://www.hielscher.com/ultrasonic-sample-prep-for-elisa-assays.htm
Protocol for SARS-CoV-2 Coronavirus Inactivation with Sonication
The Hielscher VialTweeter is a unique ultrasonic multi-sample preparation unit, which is used to inactivate the coronavirus SARS-COV-2. The VialTweeter allow to prepare up to 10 sample vials simultaneously and is thereby the ideal unit for mass sample processing.…
https://www.hielscher.com/protocol-for-sars-cov-2-coronavirus-inactivation-with-sonication.htm
Sample Preparation with the Ultrasonic VialTweeter
Sample preparation before analysis can require various pre-analytical processes such as tissue homogenization, lysis, extraction of proteins, DNA, RNA, organelles and other intracellular substances, dissolving and degassing. The VialTweeter is a unique ultrasonic device that prepares multiple sample tubes…
https://www.hielscher.com/sample-preparation-with-the-ultrasonic-vialtweeter.htm
Biosynthetic Production of Human Milk Oligosaccharides
The biosynthesis of human milk oligosaccharides (HMOs) via fermentation or enzymatic reactions is a complex, consuming and often low-yielding process. Ultrasonication increases the mass transfer between substrate and cell factories ans stimulates cell growth and metabolism. Thereby, sonication intensifies…
https://www.hielscher.com/human-milk-oligosaccharides.htm
Most Efficient Ultrasonic Dismembrators
Ultrasonic dismembrators are commonly used in the laboratory, bench-top and industry to prepare homogeneous colloidal suspensions such as emulsions and dispersions with droplet or particle sizes in the submicron- and nano-range. Furthermore, ultrasonic dismembrators are used for cell disruption,…
https://www.hielscher.com/most-efficient-ultrasonic-dismembrators.htm
Ultrasonic Disintegrators
Cell disintegration is a common task in biotechnology and in many applications that involve processing cells and intracellular material. Ultrasonic cell disintegrators are efficient and reliable, when it comes to breaking cell wall or membranes of plant cells, mammalian…
https://www.hielscher.com/ultrasonic-disintegrators.htm
Coronavirus (COVID-19, SARS-CoV-2) and Ultrasonics
Ultrasonication is a powerful tool used in biology, molecular chemistry and biochemistry as well as in the production of pharmaceuticals. Bio-sciences use ultrasonic homogenizers to lyse cells and extract proteins and other intracellular materials, the pharma industry applied ultrasonics…
https://www.hielscher.com/coronavirus-covid-19-sars-cov-2-and-ultrasonics.htm
Ultrasonics in Virus Research
Ultrasonic lysis and extraction is a reliable and long-time established method for the disruption of cells and the subsequent release of viruses, viral proteins, DNA, and RNA. Ultrasonics in Coronavirus Research The extraction of viruses from organ tissue is…
https://www.hielscher.com/ultrasonics-virus-research.htm
Ultrasonic Cold-Water Extraction of Mogrosides from Monk Fruit
Monk fruit (S. grosvenorii) extract and particularly its compound mogroside V is used as a natural non-caloric sweetener. Mogrosides. Ultrasonic extraction is a simple, rapid, reproducible, and reliable extraction method successfully applied to produce natural non-caloric sweetener from monk…
https://www.hielscher.com/ultrasonic-cold-water-extraction-of-mogrosides-from-monk-fruit.htm
Ultrasonically Enhanced Three-Phase Partitioning
Three-phase partitioning (TPP) is a technique to extract, separate and purify components, e.g lipids, enzymes, polysaccharides and other biomolecules, from biological material. The ultrasonically assisted three-phase partitioning excels the conventional TPP by higher yield, improved purity and exceptional speed.…
https://www.hielscher.com/ultrasonically-enhanced-three-phase-partitioning.htm