Utrasonic topic: "lysis"
Lysis is the term which describes the disruption of cell walls or membranes. Ultrasonic disruptors are a reliable tool to break cells in order to release intracellular compounds such as DNA, proteins, organelles, and phytochemicals. For ultrasonic lysis (aka cell disruption), high-intensity / low-frequency ultrasound waves are applied to disrupt the cell membranes. Since ultrasonication promotes mass transfer, the intracellular material is released into the surrounding solvent. The fluid containing the contents of lysed cells is called a lysate.
Cell lysis is the first step in cell fractionation, organelle isolation and protein extraction and purification.
Read more about ultrasonic cell disruptors and ultrasonic lysis!
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Monk fruit (S. grosvenorii) extract and particularly its compound mogroside V is used as a natural non-caloric sweetener. Mogrosides. Ultrasonic extraction is a simple, rapid, reproducible, and reliable extraction method successfully applied to produce natural non-caloric sweetener from monk…https://www.hielscher.com/ultrasonic-cold-water-extraction-of-mogrosides-from-monk-fruit.htm
Three-phase partitioning (TPP) is a technique to extract, separate and purify components, e.g lipids, enzymes, polysaccharides and other biomolecules, from biological material. The ultrasonically assisted three-phase partitioning excels the conventional TPP by higher yield, improved purity and exceptional speed.…https://www.hielscher.com/ultrasonically-enhanced-three-phase-partitioning.htm
Psilocybin is the active substance extracted from hallucinogenic mushrooms. Ultrasonic extraction is often used to extract the psilocybin efficiently from mushrooms. Ultrasonic extraction gives high psilocybin yield in a very rapid extraction time. Ultrasonic Isolation of Psilocybin Ultrasonic extraction…https://www.hielscher.com/ultrasonic-psilocybin-extraction-from-mushrooms.htm
Ultrasonic extraction features many advantages such as high yields, fast extraction rates, environmental-friendliness and low energy consumption. One of the strongest benefits is the use of water as extraction medium. However, sonication can be used with manifold solvent systems…https://www.hielscher.com/solvents-for-ultrasonic-extraction-from-plants.htm
During DNA and RNA shearing, DNA molecules are broken into smaller pieces. DNA / RNA fragmentation is one of the important sample prep steps required create libraries for next generation sequencing (NGS). Ultrasonic DNA shearing uses the forces of…https://www.hielscher.com/ultrasonic-dna-shearing.htm
E. coli bacteria are the most commonly used bacteria in microbiology and biotechnology. Ultrasonic cell disruptors deliver reliable and reproducible results for the lysis of E. coli. Intense yet precisely controllable cavitation and shear forces result in complete disruption…https://www.hielscher.com/ultrasonic-lysis-of-e-coli.htm
Platelet-rich plasma (PRP) is blood plasma that has been enriched with platelets, which is used for medical and estethic treatments. Ultrasonic lysis and conditioning is an very effective method to prepare platelet-rich plasma. Hielscher Ultrasonics offers various ultrasonic systems…https://www.hielscher.com/ultrasonic-preparation-of-platelet-rich-serum.htm
Ultrasonic cavitation is a highly effective mechanical method to isolate stem cells from human adipose tissue. Ultrasonically isolated stromal vascular fraction (SVF) show a high regenerative potential for medical applications. Hielscher Ultrasonics offers various options of direct and indirect…https://www.hielscher.com/ultrasonic-stem-cell-isolation.htm
Ultrasonic extraction is a green, environmental-friendly method of extraction that can be applied to small lab samples as well as for the extraction of valuable compounds on commercial production scale. The United State Environmental Protection Agency (EPA) recommends a…https://www.hielscher.com/epa3550-ultrasonic-extraction-guide.htm
Microbial deterioration can damage both water- and solvent-borne paint formulations. Environmental regulations restrict the use of biocides. Sonication is an environmental-friendly, effective method of paint preservation. Power ultrasound allows even for the recycling of microbially spoilt paints and coatings.…https://www.hielscher.com/power-ultrasound-for-advanced-microbial-stability-of-paints.htm
The ultrasonic treatment system has demonstrated to be effective in the killing of bacteria, plankton, and larger organisms. The ultrasonic disinfection of ballast water is a mechanical/ physical treatment, which avoids the dosing of any strong and expensive active…https://www.hielscher.com/ultrasonic-ballast-water-disinfection.htm
Ultrasonic extraction or sono-extraction is a process intensifying technology, which works by the coupling of high power ultrasonics into a slurry of plant or cell tissue. Hielscher Ultrasonics supplies reliable ultrasonicators for cell disruption and extraction from small lab…https://www.hielscher.com/ultrasonic-extraction-of-cellular-matter.htm
Many processes as mixing, dispersing and extraction of high-value materials are carried out as batch process. Hielscher's closed ultrasonic reactor is ideal for an integrated ultrasonic processing under closed and precisely controlled conditions. Hielscher's closed ultrasonic reactor is perfectly…https://www.hielscher.com/closed-ultrasonic-reactor-for-pharmaceutical-batch-processing.htm
Ultrasonication is a safe and efficient method of tissue homogenization. Ultrasonic applications include the preparation tissue and emulsions, the extraction of proteins, DNA/ RNA and active compounds, or the activation/ inactivation of enzymes and yeast. Precise controllability of the ultrasound…https://www.hielscher.com/ultrasonic-tissue-homogenizers.htm
Before purification or characterization of intracellular macromolecules such as proteins, organelles, enzymes or active compounds, an efficient method for tissue lysis and cell disintegration is required. Ultrasonication is a effective method for cell preparation that releases the intracellular material…https://www.hielscher.com/how-to-use-hielschers-ultrasonic-tissue-homogenizers.htm
In laboratories, it is often necessary to prepare multiple samples simultaneously at the same process conditions. The VialTweeter enables for the simultaneous sonication of up to 10 vials at the same intensity. Thereby, the VialTweeter is a reliable lab homogenizer…https://www.hielscher.com/up200st-with-vialtweeter-for-simultaneous-sample-preparation.htm
Cell disintegration or lysis is a common part of daily sample preparation in biotech laboratories. The goal of lysis is to disrupt parts of the cell wall or the complete cell to release biological molecules. The so-called lysate can…https://www.hielscher.com/ultrasonic-lysis-cell-disruption-extraction.htm
Algae Cultivation Algae Grow Lab developed a series of tubular and flat photobioreactors for algae cultivation as well as a process of cell ultrasonic destruction based on Hielscher ultrasonic processors equipped with flow cells. The general flow diagram of…https://www.hielscher.com/algae-grow-lab.htm
The UIP1000hdT (1000W, 20kHz) is a powerful and adaptable ultrasonic device for lab testing and industrial processing of liquids. It is used for applications, such as emulsification, dispersing & particle fine milling, lysis & extraction, dissolving or sonochemical reactions.…https://www.hielscher.com/i1000_p.htm
The ultrasonic processor UIP2000hd (20kHz, 2000W) is a powerful and adaptable ultrasonic device for the industrial processing of liquids. It is used for applications, such as emulsification, dispersing & particle fine milling, lysis & extraction or homogenizing.…https://www.hielscher.com/i2000_p.htm
The ultrasonic processor UIP500hdT (20kHz, 500W) is an industrial grade device for pilot testing and small scale processing of liquids. Amongst the most common applications of the UIP500hdT are homogenization, dispersing, emulsification, cell disintegration and sonochemical reactions. A full…https://www.hielscher.com/i500_p.htm
The disintegration of cell structures (lysis) by means of ultrasound is used for the extraction of intra-cellular compounds or for the microbial inactivation. Background In microbiology, ultrasound is primarily associated with cell disruption (lysis) or disintegration (Allinger…https://www.hielscher.com/extraction_01.htm
400 Watts ultrasonic power - The UP400S (400W, 24kHz) is a powerful and reliable ultrasonic device for the sonication of larger samples in the lab. Typical application include: Homogenization, deagglomeration, lysis and cell disintegration, protein extraction and the emulsification of…https://www.hielscher.com/400s_p.htm
The ultrasonic processor UP50H is typically used for the sonication of small lab samples. This homogenizer allows for the handheld or stand-mounted emulsification, dispersing, dissolving or cell disruption. The ultrasonic processor UP50H (50 watts, 30kHz) is the…https://www.hielscher.com/50h_p.htm