Utrasonic topic: "lysis"
Lysis is the term which describes the disruption of cell walls or membranes. Ultrasonic disruptors are a reliable tool to break cells in order to release intracellular compounds such as DNA, proteins, organelles, and phytochemicals. For ultrasonic lysis (aka cell disruption), high-intensity / low-frequency ultrasound waves are applied to disrupt the cell membranes. Since ultrasonication promotes mass transfer, the intracellular material is released into the surrounding solvent. The fluid containing the contents of lysed cells is called a lysate.
Cell lysis is the first step in cell fractionation, organelle isolation and protein extraction and purification.
Read more about ultrasonic cell disruptors and ultrasonic lysis!
12 pages about this topic are being shown:
Ultrasonic Cold-Water Extraction of Mogrosides from Monk Fruit
Monk fruit (S. grosvenorii) extract and particularly its compound mogroside V is used as a natural non-caloric sweetener. Mogrosides. Ultrasonic extraction is a simple, rapid, reproducible, and reliable extraction method successfully applied to produce natural non-caloric sweetener from monk…
https://www.hielscher.com/ultrasonic-cold-water-extraction-of-mogrosides-from-monk-fruit.htm
Ultrasonically Enhanced Three-Phase Partitioning
Three-phase partitioning (TPP) is a technique to extract, separate and purify components, e.g lipids, enzymes, polysaccharides and other biomolecules, from biological material. The ultrasonically assisted three-phase partitioning excels the conventional TPP by higher yield, improved purity and exceptional speed.…
https://www.hielscher.com/ultrasonically-enhanced-three-phase-partitioning.htm
Ultrasonic Psilocybin Extraction from Mushrooms
Psilocybin is the active substance extracted from hallucinogenic mushrooms. Ultrasonic extraction is often used to extract the psilocybin efficiently from mushrooms. Ultrasonic extraction gives high psilocybin yield in a very rapid extraction time. Ultrasonic Isolation of Psilocybin Ultrasonic…
https://www.hielscher.com/ultrasonic-psilocybin-extraction-from-mushrooms.htm
Solvents for Ultrasonic Extraction from Plants
Ultrasonic extraction features many advantages such as high yields, fast extraction rates, environmental-friendliness and low energy consumption. One of the strongest benefits is the use of water as extraction medium. However, sonication can be used with manifold solvent systems…
https://www.hielscher.com/solvents-for-ultrasonic-extraction-from-plants.htm
Ultrasonic DNA Shearing
During DNA and RNA shearing, DNA molecules are broken into smaller pieces. DNA / RNA fragmentation is one of the important sample prep steps required create libraries for next generation sequencing (NGS). Ultrasonic DNA shearing uses the forces of…
https://www.hielscher.com/ultrasonic-dna-shearing.htm
Ultrasonic Lysis of E. Coli
E. coli bacteria are the most commonly used bacteria in microbiology and biotechnology. Ultrasonic cell disruptors deliver reliable and reproducible results for the lysis of E. coli. Intense yet precisely controllable cavitation and shear forces result in complete disruption…
https://www.hielscher.com/ultrasonic-lysis-of-e-coli.htm
Ultrasonic Preparation of Platelet Rich Serum
Platelet-rich plasma (PRP) is blood plasma that has been enriched with platelets, which is used for medical and estethic treatments. Ultrasonic lysis and conditioning is an very effective method to prepare platelet-rich plasma. Hielscher Ultrasonics offers various ultrasonic systems…
https://www.hielscher.com/ultrasonic-preparation-of-platelet-rich-serum.htm
Ultrasonic Stem Cell Isolation
Ultrasonic cavitation is a highly effective mechanical method to isolate stem cells from human adipose tissue. Ultrasonically isolated stromal vascular fraction (SVF) show a high regenerative potential for medical applications. Hielscher Ultrasonics offers various options of direct and indirect…
https://www.hielscher.com/ultrasonic-stem-cell-isolation.htm
EPA3550 Ultrasonic Extraction Guide
Ultrasonic extraction is a green, environmental-friendly method of extraction that can be applied to small lab samples as well as for the extraction of valuable compounds on commercial production scale. The United State Environmental Protection Agency (EPA) recommends a…
https://www.hielscher.com/epa3550-ultrasonic-extraction-guide.htm
Power-Ultrasound for Advanced Microbial Stability of Paints
Microbial deterioration can damage both water- and solvent-borne paint formulations. Environmental regulations restrict the use of biocides. Sonication is an environmental-friendly, effective method of paint preservation. Power ultrasound allows even for the recycling of microbially spoilt paints and coatings.…
https://www.hielscher.com/power-ultrasound-for-advanced-microbial-stability-of-paints.htm
Ultrasonic Ballast Water Disinfection
The ultrasonic treatment system has demonstrated to be effective in the killing of bacteria, plankton, and larger organisms. The ultrasonic disinfection of ballast water is a mechanical/ physical treatment, which avoids the dosing of any strong and expensive active…
https://www.hielscher.com/ultrasonic-ballast-water-disinfection.htm
Ultrasonic Extraction of Cellular Matter
Ultrasonic extraction or sono-extraction is a process intensifying technology, which works by the coupling of high power ultrasonics into a slurry of plant or cell tissue. Hielscher Ultrasonics supplies reliable ultrasonicators for cell disruption and extraction from small lab…
https://www.hielscher.com/ultrasonic-extraction-of-cellular-matter.htm