Ultrasonic topic: "Ultrasonic Lysis and Cell Disruption"
Lysis is the term which describes the disruption of cell walls or membranes. Ultrasonic disruptors are a reliable tool to break cells in order to release intracellular compounds such as DNA, proteins, organelles, and phytochemicals. For ultrasonic lysis (aka cell disruption), high-intensity / low-frequency ultrasound waves are applied to disrupt the cell membranes. Since ultrasonication promotes mass transfer, the intracellular material is released into the surrounding solvent. The fluid containing the contents of lysed cells is called a lysate.
Cell lysis is the first step in cell fractionation, organelle isolation and protein extraction and purification.
Read more about ultrasonic cell disruptors and ultrasonic lysis!
Found 58 hits. Showing results 13 - 24.
96-Well Sonicators – Streamline High-Throughput Sample Preparation
The 96-well sonicator UIP400MTP is a high-power ultrasound device designed specifically for the effective sonication of multi-well plates. The UIP400MTP is designed to enable precise, uniform focused sonication across all wells in microtiter plates, widely used in laboratory settings for…
https://www.hielscher.com/96-well-sonicator.htm
Focused Sonicators for High-Throughput Sample Processing
Focused sonicators are the solution for high-throughput sample preparation. The 96-Well Plate Sonicator UIP400MTP reliably processes samples in multi-well plates, 96-well plates, Petri dishes or test tubes and are specifically designed to meet the rigorous demands of laboratories and research…
https://www.hielscher.com/focused-sonicators-for-high-throughput-sample-processing.htm
Assay Sonication – The Assay-at-Once Sonicator UIP400MTP
Sonication is an essential technique in laboratory protocols, particularly for applications like chromatin immunoprecipitation (ChIP) assays. By using ultrasonic energy to break down cells, shear DNA, and solubilize chromatin, sonication enables researchers to prepare samples more efficiently and reproducibly. The…
https://www.hielscher.com/assay-sonication-the-assay-at-once-sonicator-uip400mtp.htm
Sonication Devices for your Lab Tasks
Sonication devices, particularly probe-type sonicators, are highly specialized tools widely employed in both laboratory and industrial settings. These instruments harness the power of ultrasound to efficiently process various materials. By transmitting ultrasonic energy through a probe (or horn) into a…
https://www.hielscher.com/sonication-devices-for-your-lab-tasks.htm
Microbiome Research and Clinical Analysis Streamlined by Sonication
Sonication is a powerful technique widely used in microbiome research and clinical analysis facilitating a high-throughput sample preparation. The ultrasonic method is particularly effective for processing complex biological samples, such as those containing microorganisms. The use of sonication facilitates cell…
https://www.hielscher.com/microbiome-reasearch-sonication.htm
High-Throughput Sample Preparation for Mitochondrial Diagnostics
Mitochondrial diagnostics in research and clinics are performed using various techniques such as sequencing, PCR, and biochemical assays. These methods are used to identify DNA mutations and measure mitochondrial function. Sequencing helps detect genetic mutations, while PCR can quantify specific…
https://www.hielscher.com/mitochondrial-diagnostic.htm
Genomic Research Facilitated by Sonication
Genomic research has revolutionized our understanding of biological systems, enabling the elucidation of complex genetic mechanisms underlying various diseases and traits. Sonication, a technique originally developed for the disruption of cellular membranes, has found extensive utility in genomic research. This…
https://www.hielscher.com/genomic-research-facilitated-by-sonication.htm
Ultrasonic Lysis: Step-by-Step Guide to Perfecting Cell Disruption
Do you want to master the science of cell lysis? Look no further! In this step-by-step guide, we will walk you through the process of ultrasonic cell disruption and ensure that your cell lysis technique gets you optimal results. Whether…
https://www.hielscher.com/ultrasonic-lysis-step-by-step-guide-to-perfecting-cell-disruption.htm
Plasmid Preparation using Ultrasonication
Ultrasonication is a reliable technique to fragment plasmid DNA. Precisely controllable amplitude, pulsation mode and temperature control are most important features of an ultrasonicator for non-damaging plasmid fragmentation. Additionally, the use of certain agents help to protect against plasmid degradation.…
https://www.hielscher.com/plasmid-preparation-using-ultrasonication.htm
Ultrasonic CupHorn
The Hielscher Ultrasonic CupHorn is the world's most advanced ultrasonic cuphorn. The ultrasonic intensity of the Hielscher CupHorn exceeds conventional ultrasonic baths by a thousand times. Precise control and user-friendliness ensure reliable and repeatable outcomes and comfortable operation. That's why…
https://www.hielscher.com/ultrasonic-cuphorn.htm
Ultrasonic Lysis of Bioengineered Cells in Industrial Production
Bioengineered bacteria species such E. coli as well as genetically modified mammalian and plant cell types are used widely in biotech to express molecules. In order to release these synthesized bio-molecules, a reliable cell disruption technique is required. High-performance ultrasonication…
https://www.hielscher.com/ultrasonic-lysis-of-bioengineered-cells-in-industrial-production.htm
Ultrasonic DNA Fragmentation for Next Gen Sequencing
Next Generation Sequencing (NGS) requires the reliable shearing and fragmentation of genomic DNA in order to sequence genomic DNA strands and to create genome libraries. The controlled fragmentation of DNA into DNA fragments is an essential sample preparation step required…
https://www.hielscher.com/ultrasonic-dna-fragmentation-for-next-gen-sequencing.htm