Ultrasonic topic: "Ultrasonic Lysis and Cell Disruption"
Lysis is the term which describes the disruption of cell walls or membranes. Ultrasonic disruptors are a reliable tool to break cells in order to release intracellular compounds such as DNA, proteins, organelles, and phytochemicals. For ultrasonic lysis (aka cell disruption), high-intensity / low-frequency ultrasound waves are applied to disrupt the cell membranes. Since ultrasonication promotes mass transfer, the intracellular material is released into the surrounding solvent. The fluid containing the contents of lysed cells is called a lysate.
Cell lysis is the first step in cell fractionation, organelle isolation and protein extraction and purification.
Read more about ultrasonic cell disruptors and ultrasonic lysis!
Found 56 hits. Showing results 13 - 24.
Assay Sonication – The Assay-at-Once Sonicator UIP400MTP
Sonication is an essential technique in laboratory protocols, particularly for applications like chromatin immunoprecipitation (ChIP) assays. By using ultrasonic energy to break down cells, shear DNA, and solubilize chromatin, sonication enables researchers to prepare samples more efficiently and reproducibly. The…
https://www.hielscher.com/assay-sonication-the-assay-at-once-sonicator-uip400mtp.htm
Sonication Devices for your Lab Tasks
Sonication devices, particularly probe-type sonicators, are highly specialized tools widely employed in both laboratory and industrial settings. These instruments harness the power of ultrasound to efficiently process various materials. By transmitting ultrasonic energy through a probe (or horn) into a…
https://www.hielscher.com/sonication-devices-for-your-lab-tasks.htm
Microbiome Research and Clinical Analysis Streamlined by Sonication
Sonication is a powerful technique widely used in microbiome research and clinical analysis facilitating a high-throughput sample preparation. The ultrasonic method is particularly effective for processing complex biological samples, such as those containing microorganisms. The use of sonication facilitates cell…
https://www.hielscher.com/microbiome-reasearch-sonication.htm
High-Throughput Sample Preparation for Mitochondrial Diagnostics
Mitochondrial diagnostics in research and clinics are performed using various techniques such as sequencing, PCR, and biochemical assays. These methods are used to identify DNA mutations and measure mitochondrial function. Sequencing helps detect genetic mutations, while PCR can quantify specific…
https://www.hielscher.com/mitochondrial-diagnostic.htm
Genomic Research Facilitated by Sonication
Genomic research has revolutionized our understanding of biological systems, enabling the elucidation of complex genetic mechanisms underlying various diseases and traits. Sonication, a technique originally developed for the disruption of cellular membranes, has found extensive utility in genomic research. This…
https://www.hielscher.com/genomic-research-facilitated-by-sonication.htm
Ultrasonic Lysis: Step-by-Step Guide to Perfecting Cell Disruption
Do you want to master the science of cell lysis? Look no further! In this step-by-step guide, we will walk you through the process of ultrasonic cell disruption and ensure that your cell lysis technique gets you optimal results. Whether…
https://www.hielscher.com/ultrasonic-lysis-step-by-step-guide-to-perfecting-cell-disruption.htm
Plasmid Preparation using Ultrasonication
Ultrasonication is a reliable technique to fragment plasmid DNA. Precisely controllable amplitude, pulsation mode and temperature control are most important features of an ultrasonicator for non-damaging plasmid fragmentation. Additionally, the use of certain agents help to protect against plasmid degradation.…
https://www.hielscher.com/plasmid-preparation-using-ultrasonication.htm
Ultrasonic CupHorn
The Hielscher Ultrasonic CupHorn is the world's most advanced ultrasonic cuphorn. The ultrasonic intensity of the Hielscher CupHorn exceeds conventional ultrasonic baths by a thousand times. Precise control and user-friendliness ensure reliable and repeatable outcomes and comfortable operation. That's why…
https://www.hielscher.com/ultrasonic-cuphorn.htm
Ultrasonic Lysis of Bioengineered Cells in Industrial Production
Bioengineered bacteria species such E. coli as well as genetically modified mammalian and plant cell types are used widely in biotech to express molecules. In order to release these synthesized bio-molecules, a reliable cell disruption technique is required. High-performance ultrasonication…
https://www.hielscher.com/ultrasonic-lysis-of-bioengineered-cells-in-industrial-production.htm
Ultrasonic DNA Fragmentation for Next Gen Sequencing
Next Generation Sequencing (NGS) requires the reliable shearing and fragmentation of genomic DNA in order to sequence genomic DNA strands and to create genome libraries. The controlled fragmentation of DNA into DNA fragments is an essential sample preparation step required…
https://www.hielscher.com/ultrasonic-dna-fragmentation-for-next-gen-sequencing.htm
Cell Lysis of BL21 Cells by Ultrasonication
BL21 cells are a strain of E. coli that is widely used in research laboratories, biotechnology and industrial production due to their capability to express proteins highly efficient. Ultrasonic cell disruption, lysis and protein extraction is the common method to…
https://www.hielscher.com/cell-lysis-of-bl21-cells-by-ultrasonication.htm
Ultrasonic Sample Prep for ELISA Assays
Assays such as ELISA are widely used for in-vitro diagnostics, disease-related protein detection and quality control (e.g. monitoring food allergens). Ultrasonic sample preparation is a rapid, reliable and reproducible technique to lyse cell and isolate intracellular proteins, DNA, RNA and…
https://www.hielscher.com/ultrasonic-sample-prep-for-elisa-assays.htm