96-Well Sonicators – Streamline High-Throughput Sample Preparation
The 96-well sonicator UIP400MTP is a high-power ultrasound device designed specifically for the effective sonication of multi-well plates. The UIP400MTP is designed to enable precise, uniform focused sonication across all wells in microtiter plates, widely used in laboratory settings for tasks that require the parallel treatment of multiple samples. This sonicator is highly favored for its efficiency, versatility, and reliability, especially in fields where sample preparation and consistency are critical, such as life sciences, biotechnology, pharmacology, and clinical analysis.
Multi-well plate sonicator UIP400MTP
The 96-well sonicator UIP400MTP is applied across various laboratory tasks where uniform sample treatment is critical. The UIP400MTP will facilitate and accelerate applications such as:
- Cell Disruption and Lysis: The UIP400MTP is frequently used in microbiology and cellular biology for the controlled lysis of cells in a high-throughput manner. It effectively breaks down cell walls and membranes to release intracellular contents, which is essential for assays that require access to DNA, RNA, or proteins.
- Protein Extraction for Purification and Proteomics Research: In proteomics, precise protein extraction is essential for analyzing protein structure, function, and interactions. The UIP400MTP facilitates efficient protein extraction from complex samples, breaking down cellular structures to release proteins in a controlled manner. This is particularly valuable for purifying proteins for downstream applications such as mass spectrometry, Western blotting, and other proteomic analyses. Read more!
- Chromatin Shearing: The UIP400MTP is highly effective for chromatin shearing in epigenetics research. Consistent sonication breaks chromatin into fragments of optimal size for chromatin immunoprecipitation (ChIP) assays, making it ideal for studying DNA-protein interactions and modifications. This application is vital in research areas such as gene regulation, histone modification, and epigenetic profiling.
- Sonication of DNA and RNA: This sonicator is useful for fragmenting DNA and RNA into desired sizes, making it a valuable tool for laboratories working on next-generation sequencing and other genomic applications. Read more!
- Homogenization: The UIP400MTP is ideal for homogenizing tissue samples, suspensions, and emulsions. Its ultrasound waves break down large particles to create uniform mixtures, ensuring each well in the plate has a consistent suspension.
Take 96-Well Plate Sample Preparation to A New Level!
The 96-well plate sonicator UIP400MTP offers significant advantages for microplate sonication, providing efficient and uniform ultrasonic energy across all wells. This high-throughput system allows for simultaneous processing of all 96 samples, making it ideal for large-scale applications like cell lysis, protein extraction, or nanoparticle dispersion. With precise control over sonication parameters such as intensity and duration, the UIP400MTP ensures reproducible results and consistent sample quality.
Choose the Ideal Plate – No Expensive Proprietary Consumables!
Its compatibility with standard 96-well plates facilitates easy integration into automated workflows, enhancing productivity while reducing manual handling and contamination risks, making it a powerful tool for laboratories focused on high-efficiency sample preparation. Use the microplate, PCR, ELISA, multi-well plate of your choice. The UIP400MTP is compatible with any standard microtiter plate (6, 12, 24, 48, 96, 384 wells). The UIP400MTP works also with Petri dishes and test tubes in a tube rack!
Design, Manufacturing and Consulting – Quality Made in Germany
Hielscher ultrasonicators are well-known for their highest quality and design standards. Robustness and easy operation allow the smooth integration of our ultrasonicators into industrial facilities. Rough conditions and demanding environments are easily handled by Hielscher ultrasonicators.
Hielscher Ultrasonics is an ISO certified company and put special emphasis on high-performance ultrasonicators featuring state-of-the-art technology and user-friendliness. Of course, Hielscher ultrasonicators are CE compliant and meet the requirements of UL, CSA and RoHs.
Literature / References
- FactSheet UIP400MTP Multi-well Plate Sonicator – Non-Contact Sonicator – Hielscher Ultrasonics
- Mochizuki, Chika; Taketomi, Yoshitaka; Irie, Atsushi; Kano, Kuniyuki; Nagasaki, Yuki; Miki, Yoshimi; Ono, Takashi; Nishito, Yasumasa; Nakajima, Takahiro; Tomabechi, Yuri; Hanada, Kazuharu; Shirouzu, Mikako; Watanabe, Takashi; Hata, Kousuke; Izumi, Yoshihiro; Bamba, Takeshi; Chun, Jerold; Kudo, Kai; Kotani, Ai; Murakami, Makoto (2024): Secreted phospholipase PLA2G12A-driven lysophospholipid signaling via lipolytic modification of extracellular vesicles facilitates pathogenic Th17 differentiation. BioRxiv 2024.
- UIP400MTP-Multi-well-Plate-Sonicator-Infographic
- De Oliveira A, Cataneli Pereira V, Pinheiro L, Moraes Riboli DF, Benini Martins K, Ribeiro de Souza da Cunha MDL (2016): Antimicrobial Resistance Profile of Planktonic and Biofilm Cells of Staphylococcus aureus and Coagulase-Negative Staphylococci. International Journal of Molecular Sciences 17(9):1423; 2016.
- Martins KB, Ferreira AM, Pereira VC, Pinheiro L, Oliveira A, Cunha MLRS (2019): In vitro Effects of Antimicrobial Agents on Planktonic and Biofilm Forms of Staphylococcus saprophyticus Isolated From Patients With Urinary Tract Infections. Frontiers in Microbiology 2019.
Frequently Asked Questions
What is the Size of a 96-Well Plate?
The standard size of a 96-well plate is approximately 127.76 mm in length, 85.48 mm in width, and 14.35 mm in height, with each well having a diameter of about 6.86 mm. The plate follows the ANSI/SBS (Society for Biomolecular Sciences) standard, allowing compatibility with automated laboratory equipment.
What is a 96-Well Plate used for?
A microplate is used for a wide variety of laboratory applications that require multiple, parallel assays or tests, allowing for high-throughput analysis. Here are some of the primary uses:
- Enzyme-Linked Immunosorbent Assay (ELISA): Microplates are commonly used for ELISAs, where antibodies and antigens react to detect and quantify proteins, hormones, and other molecules in samples.
- Drug Screening and Discovery: In pharmacology and biotechnology, microplates enable high-throughput screening of compounds, allowing researchers to test thousands of chemical compounds on cells or enzymes to find potential drug candidates.
- Molecular Biology Assays: Microplates are used for assays involving DNA, RNA, or proteins, such as polymerase chain reaction (PCR), DNA quantification, and gene expression analysis.
- Cell Culture: Tissue-culture-treated microplates allow researchers to grow and monitor cells in each well, ideal for studying cell behavior, drug effects, and cytotoxicity assays.
- Biochemical Assays: These plates are also used for enzyme assays, binding studies, and kinetic analyses to measure the activity of biochemical reactions and analyze substances like metabolites or inhibitors.
- Sample Storage and Dilution: Microplates allow easy storage of multiple small-volume samples in a compact space. They are also useful for serial dilution, enabling easy concentration gradients for experiments.
- Diagnostic Testing: In clinical settings, microplates are used for various diagnostic tests, such as hormone levels, infectious disease markers, and blood typing, often conducted on automated instruments.
- Spectrophotometry and Fluorometry: With compatible plate readers, microplates enable the analysis of light absorbance, fluorescence, and luminescence for multiple samples at once, which is common in biochemical and cell-based assays.
Microplates have become essential in laboratories because they streamline workflows, reduce sample and reagent use, and allow compatibility with automation for efficient, high-throughput analysis.
What are Commonly Used 96-well Plate Brands?
Several manufacturers produce 96-well plates, each offering various features tailored to specific laboratory applications. Some of the most commonly used brands include:
- BRAND®: Known for their high-quality microplates, BRAND offers the BRANDplates® series, which includes pureGrade™ plates suitable for homogeneous assays, screenings, and storage. These plates are available in different colors and well shapes to meet diverse experimental needs.
- Corning®: A leading supplier of laboratory consumables, Corning provides a wide range of 96-well plates, including clear, black, and white plates with various surface treatments for cell culture, fluorescence, and luminescence assays.
- Thermo Scientific™ (Nunc™): Offers a comprehensive selection of 96-well plates designed for applications such as ELISA, cell culture, and high-throughput screening. Their plates come in different materials and surface treatments to suit specific assay requirements.
- Greiner Bio-One: Provides 96-well plates under the brand name Greiner, featuring various surface treatments and well shapes. These plates are widely used in cell culture, immunology, and molecular biology applications.
- Eppendorf: Known for their high-quality laboratory products, Eppendorf offers 96-well plates suitable for PCR, qPCR, and other molecular biology applications, ensuring reliable performance and reproducibility.
When selecting a 96-well plate, it’s essential to consider factors such as material, surface treatment, well shape, and compatibility with your specific assays and detection instruments.
Hielscher Ultrasonics manufactures high-performance ultrasonic homogenizers from lab to industrial size.