PCR Plate Sonicator UIP400MTP
The PCR Plate Sonicator UIP400MTP is designed to optimize workflows involving PCR plates, streamlining PCR tests with precision and efficiency. This innovative equipment enables uniform ultrasonic treatment of entire multi-well plates, making it ideal for tasks such as sample preparation, cell lysis, DNA shearing, and mixing reagents directly within PCR wells. By eliminating manual handling and ensuring consistent results across all wells, the UIP400MTP reduces variability, enhances reproducibility, and saves valuable time in high-throughput PCR workflows. Its user-friendly interface and adjustable sonication parameters provide researchers with precise control, making it an indispensable tool for modern molecular biology laboratories.
Improve PCR Workflows
The UIP400MTP microplate sonicator enhances precision, efficiency, and reproducibility in workflows involving PCR plates. Ideal for molecular biology, diagnostics, and biotechnology, this 96-well plate sonicator streamlines high-throughput applications and supports consistent, reliable results for demanding laboratory tasks.
High-throughput PCR assays with the 96-well plate sonicator UIP400MTP
How the Hielscher UIP400MTP Enhances PCR and qPCR Plate Workflows
The Hielscher UIP400MTP microplate sonicator enhances a variety of assays conducted in PCR and qPCR plates by improving sample preparation and ensuring uniformity. Some commonly used assays that benefit from sonication include:
- Nucleic Acid Extraction and Purification
Sonication aids in efficient cell lysis, releasing DNA and RNA from cells or tissues for downstream analysis.
Improves the yield and quality of nucleic acids by breaking up tough cell walls or biofilms. - DNA Shearing for Next-Generation Sequencing (NGS)
Ensures precise fragmentation of DNA to the required sizes for library preparation in sequencing workflows. - Gene Expression Analysis
For assays like reverse transcription PCR (RT-PCR) or quantitative PCR (qPCR), sonication ensures thorough mixing of reagents, primers, and templates, reducing pipetting errors and variability. - Pathogen Detection
Breaks down viral or bacterial cells to release genetic material for identification, particularly in diagnostic assays such as detecting SARS-CoV-2 or other infectious agents. - Protein-DNA or Protein-RNA Interaction Studies
Helps in chromatin immunoprecipitation (ChIP) assays or RNA immunoprecipitation (RIP) by breaking up chromatin and lysing cells efficiently. - High-Throughput Screening
Enables consistent and reliable processing of multiple samples in drug discovery, biomarker research, or molecular diagnostics. - Microbial Community Analysis
Facilitates the lysis of microbial cells in complex samples, such as environmental or clinical microbiomes, for metagenomic studies.
By ensuring uniform sonication across all wells, the UIP400MTP reduces variability, enhances reproducibility, and streamlines workflows, making it an invaluable tool for laboratories running these and other molecular biology assays.
The multi-well plate sonicator UIP400MTP facilitates high-throughput sample preparation streamlining research work and assays.
Key Features of the UIP400MTP PCR Plate Sonicator
| Uniform Sonication Across PCR Plates | Compatible with any 96- and 384-well or other multi-well formats, Petri dishes and tube racks. Ensures consistent treatment across all wells for reproducible results. |
| Use the Multiwell Plate of Your Choice | The UIP400MTP is designed to sonicate any standard multiwell plates, PCR plates, or microtiter plates. You choose the plate most suitable for your experiments. As the UIP400MTP does not require proprietary consumables, this saves you a lot of money! |
| Versatile Applications | Perfect for cell lysis, DNA shearing, protein extraction, cell detachment, biofilm dislodging, homogenization, and reagent mixing. Processes samples directly within the PCR plate, eliminating transfer steps. |
| Customizable Parameters | Adjustable amplitude and duration settings. Match sonication to the specific needs of delicate assays or challenging samples. |
| Efficient and Scalable | Saves time with high-throughput processing. Scalable for projects of all sizes, supporting modern laboratory workflows. Suitable for automation. |
| Temperature Control | Set a specific temperature for your sonication. The UIP400MTP plate sonicator can monitor sample temperature and adjust the sonication accordingly. A recirculating cooling liquid chills each well in the plate for precise temperature control. |
| Protocoling and Remote Control | Each sonication run can be controlled from a computer. No software installation is needed. You can use your standard browser (e.g., Chrome, Firefox, Edge, or Safari) on any operating system (e.g., MacOS, Windows, Android, iOS, or Linux) to control and monitor the UIP400MTP remotely. Each sonication run produces a detailed sonication protocol for evaluation and documentation. The protocol file is in a non-proprietary CSV format, which you can open with your preferred program, such as Microsoft Excel, Apple Numbers, or OpenOffice Calc. |
The UIP400MTP ensures reliable sample preparation and a facile integration with existing lab workflows
PCR PLate Sonicator UIP400MTP
Laboratories need reliable, efficient, and precise tools to enhance PCR plate workflows and to get robust, reproducible results. Fulfilling tasks such as cell lysis, DNA shearing, protein extraction, cell detachment, biofilm dislodging, and homogenization at high-throughput with efficiency and precision, the UIP400MTP multi-well plate sonicator is an essential addition for any laboratory handling PCR plates. With advanced ultrasonic capabilities, the Hielscher UIP400MTP transforms traditional sample preparation methods, saving time and improving the quality of results across a wide range of molecular applications.
Design, Manufacturing and Consulting – Quality Made in Germany
Hielscher ultrasonicators are well-known for their highest quality and design standards. Robustness and easy operation allow the smooth integration of our ultrasonicators into industrial facilities. Rough conditions and demanding environments are easily handled by Hielscher ultrasonicators.
Hielscher Ultrasonics is an ISO certified company and put special emphasis on high-performance ultrasonicators featuring state-of-the-art technology and user-friendliness. Of course, Hielscher ultrasonicators are CE compliant and meet the requirements of UL, CSA and RoHs.
The microplate sonicator UIP400MTP handles any standard assay plates
Literature / References
- FactSheet UIP400MTP Multi-well Plate Sonicator – Non-Contact Sonicator – Hielscher Ultrasonics
- Lauren E. Cruchley-Fuge, Martin R. Jones, Ossama Edbali, Gavin R. Lloyd, Ralf J. M. Weber, Andrew D. Southam, Mark R. Viant (2024): Automated extraction of adherent cell lines from 24-well and 96-well plates for multi-omics analysis using the Hielscher UIP400MTP sonicator and Beckman Coulter i7 liquid handling workstation. Metabomeeting 2024, University of Liverpool, 26-28th November 2024.
- Dreyer J., Ricci G., van den Berg J., Bhardwaj V., Funk J., Armstrong C., van Batenburg V., Sine C., VanInsberghe M.A., Marsman R., Mandemaker I.K., di Sanzo S., Costantini J., Manzo S.G., Biran A., Burny C., Völker-Albert M., Groth A., Spencer S.L., van Oudenaarden A., Mattiroli F. (2024): Acute multi-level response to defective de novo chromatin assembly in S-phase. Molecular Cell 2024.
- Mochizuki, Chika; Taketomi, Yoshitaka; Irie, Atsushi; Kano, Kuniyuki; Nagasaki, Yuki; Miki, Yoshimi; Ono, Takashi; Nishito, Yasumasa; Nakajima, Takahiro; Tomabechi, Yuri; Hanada, Kazuharu; Shirouzu, Mikako; Watanabe, Takashi; Hata, Kousuke; Izumi, Yoshihiro; Bamba, Takeshi; Chun, Jerold; Kudo, Kai; Kotani, Ai; Murakami, Makoto (2024): Secreted phospholipase PLA2G12A-driven lysophospholipid signaling via lipolytic modification of extracellular vesicles facilitates pathogenic Th17 differentiation. BioRxiv 2024.
- Cosenza-Contreras M, Seredynska A, Vogele D, Pinter N, Brombacher E, Cueto RF, Dinh TJ, Bernhard P, Rogg M, Liu J, Willems P, Stael S, Huesgen PF, Kuehn EW, Kreutz C, Schell C, Schilling O. (2024): TermineR: Extracting information on endogenous proteolytic processing from shotgun proteomics data. Proteomics. 2024.
- De Oliveira A, Cataneli Pereira V, Pinheiro L, Moraes Riboli DF, Benini Martins K, Ribeiro de Souza da Cunha MDL (2016): Antimicrobial Resistance Profile of Planktonic and Biofilm Cells of Staphylococcus aureus and Coagulase-Negative Staphylococci. International Journal of Molecular Sciences 17(9):1423; 2016.
- Martins KB, Ferreira AM, Pereira VC, Pinheiro L, Oliveira A, Cunha MLRS (2019): In vitro Effects of Antimicrobial Agents on Planktonic and Biofilm Forms of Staphylococcus saprophyticus Isolated From Patients With Urinary Tract Infections. Frontiers in Microbiology 2019.
Frequently Asked Questions
What is a PCR Plate?
A PCR plate is a flat, multi-well plate designed for thermal cycling and amplification of nucleic acids in PCR assays, typically made of plastic to ensure efficient heat transfer.
How Many Wells are in a PCR plate?
A standard PCR plate has 96 or 384 wells, although other configurations are also available.
What is PCR used for?
PCR is used for amplifying specific DNA or RNA sequences to detect, quantify, or analyze genetic material.
What is the meaning of PCR in Laboratory?
In the laboratory, PCR stands for Polymerase Chain Reaction, a method for enzymatic amplification of nucleic acids.
What does a PCR Test for?
A PCR test detects the presence or absence of specific genetic material, often to identify pathogens or genetic variations.
What Diseases can PCR Detect?
PCR can detect diseases such as COVID-19, HIV, tuberculosis, and genetic disorders, among others.
How is PCR used to Identify Bacteria?
PCR identifies bacteria by amplifying unique DNA sequences that are specific to the bacterial species being tested.
What is an Example of a PCR?
An example of a PCR is reverse transcription PCR (RT-PCR) used for detecting RNA viruses like SARS-CoV-2.
Is PCR a DNA or RNA test?
PCR can test for both DNA and RNA, with RNA first being converted into complementary DNA (cDNA) using reverse transcription.
How does PCR work Step-by-Step?
PCR works step by step through denaturation (heating to separate DNA strands), annealing (cooling to allow primers to bind to target sequences), and extension (using a DNA polymerase to synthesize new DNA strands). This cycle is repeated multiple times to amplify the target sequence.
Hielscher Ultrasonics manufactures high-performance ultrasonic homogenizers from lab to industrial size.