Ultrasonic Tissue Homogenizers
- Ultrasonication is a safe & efficient method of tissue homogenization.
- Ultrasonic applications include the preparation tissue and suspensions, the extraction of proteins, DNA/ RNA and active compounds, as well as the activation / inactivation of enzymes and yeasts.
- The precise controllability of probe-type ultrasonic homogenizers allow for a reliable preparation of tissue samples.
Tissue Homogenization using Ultrasonic Probes
Tissue homogenization is a sample preparation process where animal and plant cells or microorganisms are prepared prior to the extraction of intracellular matter such as proteins, DNA, or RNA. The cell membran must be ruptured to release the cell contents. After breaking the cell wall, the intracellular macromolecules float in the buffer solution so that organelles, proteins and DNA/ RNA become available. Ultrasonic tissue homogenizers and cell disruptors are very efficient tools for cell disruption, lysis and extraction.
Ultrasonic Tissue Homogenizers vs Alternative Lysis Techniques
You might ask yourself what technique of cell disruption and which tissue homogenization equipment is the best option for your laboratory. Below, you can find a few arguments that speak for ultrasonic lysis and tissue homogenization since ultrasonication has several advantages over alternative homogenization methods:
- Efficiency: Ultrasonic homogenization is a fast and efficient method for disrupting tissues and cells. Ultrasonic tissue homogenizers use the working principle of acoustic cavitation. This method is highly efficient and can disrupt cells in a matter of seconds. As the intensity level can be adjusted from mild to intense, sonication is particularly effective for hard or fibrous tissues that are difficult to homogenize using other methods.
- Avoid cross-contamination: Hielscher offers powerful ultrasonic tissue homogenizers that prepare the sample in an indirect sonication treatment. With the UIP400MTP (ideal for microtiter and multiwell-plates), as well as the VialTweeter and the CupHorn (Ideal for vials, tubes and beakers) Hielscher offers ultrasonic cell disruption equipment for non-contact homogenization, which reduces the risk of cross-contamination between samples. It also minimizes the risk of introducing contaminants from grinding surfaces or other homogenization equipment.
- Flexibility: Ultrasonic homogenization can be used with a variety of sample sizes and volumes, from small volumes to large tissue samples. Furthermore, ultrasonic homogenization also suitable for a wide range of sample types, including plant tissues, animal tissues, and microbial cultures. The intensity and duration of the ultrasonication can be adjusted to optimize the level of disruption for different types of tissues.
- Reproducibility: Ultrasonic homogenization provides consistent results and allows for the precise control of homogenization parameters, such as power output and sonication time. This helps ensure reproducibility and accuracy of experimental data.
- Preservation of biomolecules: Probe-type ultrasonication is a non-thermal treatment. This means its impact is not based on heat. However, every mechanical treatment generates heat in accordance with the second law of thermodynamics. This means that the sample gets warmer over longer sonication times. Hielscher state-of-the-art ultrasonicators are equipped with temperature sensors and smart temperature monitoring software. Sophisticated cooling options allow to prevent heat degradation and helps to preserve the activity of heat-sensitive biomolecules, such as enzymes and proteins.
- Time-saving: The high efficiency of probe-type ultrasonication allows for rapid tissue homogenization, which saves time compared to other methods that require more extensive physical manipulation of the tissue.
In summary, ultrasonic tissue homogenization offers several advantages over alternative homogenization methods. That is why ultrasonic tissue homogenizers are the preferred cell disruption technique in various research and industrial applications.
Example for a Tissue Homogenization Protocol using an Ultrasonic Probe:
Ultrasonicators facilitate tissue homogenization by simple operation and a quick homogenization procedure. At the same time, the ultrasonic process parameters can be recisely controlled and monitored so that results are reliable and reproduicble.
- Tissue sample (e.g. liver, brain, muscle)
- Buffer (e.g. PBS, Tris-HCl)
- Protease inhibitor cocktail
- Ultrasonic homogenizer (probe-type)
- Microcentrifuge tubes
Protocol for Ultrasonic Tissue Homogenization:
Note: The duration and intensity of sonication may need to be optimized depending on the tissue type, age, and health status, as well as the specific downstream applications. It is also important to carefully select the buffer, protease inhibitors, and other additives to optimize the yield and quality of the homogenate.
Major Advantages of Hielscher Ultrasonic Tissue Homogenizers
- rapid and efficient grinding, homogenization, lysis & cell disruption, or extraction by powerful ultrasonic cavitation
- easy and intuitive use and operation (select the analog or digital version)
- easy cleaning of product-contact surfaces by flushing the sonotrode under sonication (CIP clean-in-place/ SIP sterilize-in-place)
- sound protection box (the sound protection box for the lab devices are completely made from acrylic glass for full visibility from any angle)
- full control over the process parameters via digital touch-display at the device (digital ultrasonicators) or powermeter (for analog devices)
- integrated temperature control (important for heat-sensitive, labile tissue samples)
- pauses in the homogenization run can be individually defined and pre-set in the digital menu
- all digital devices are equipped with automatic data recording (on an integrated SD/USB-ComboCard), plugable temperature sensor and browser remote control.
- multi-sample treatment with the VialTweeter, CupHorn or by the use of 4- or 8-finger sonotrodes (e.g. for integration in laboratory robot processes)
- for small scale and large scale applications
The table below gives you an indication of the approximate processing capacity of our ultrasonic tissue homogenizers for cell disintegration, lysis and extraction:
|Batch Volume||Flow Rate||Recommended Devices|
|vials, small beaker||n/a||Ultrasonic CupHorn|
|up to 10 vials||n/a||VialTweeter|
|1 to 500mL||10 to 200mL/min||UP100H|
|10 to 2000mL||20 to 400mL/min||UP200Ht, UP400St|
|0.1 to 20L||0.2 to 4L/min||UIP2000hdT|
Contact Us! / Ask Us!
Find the Optimal Ultrasonic Tissue Homogenizer for Your Application
Hielscher ultrasonic tissue homogenizers are available with an ultrasound output range between 50W to 16,000W (16kW).
Laboratory workers are pleased by the simple and intuitive operation of Hielscher ultrasonic lab homogenizers. Depending on the specific requirements, Hielscher devices come with digital or analog control.
The VialTweeter is a powerful ultrasonic device that allows the simultaneous sonication of up to 10 vials or test tubes at the same intensity. Due to the indirect sonication, cross-contamination and sample loss is completely avoided. The cleaning and sanitizing is reduced to a minimum due to the use of disposable vials. The block sonotrode is autoclavable.
In the range of probe-type lab ultrasonicators, devices can be selected with 50W, 100W, 200W, 250W and 400W.
For larger process volumes, the bench-top ultrasonic disruptors are available with 500W, 1000W, 1500W and 2000W.
And for high volume streams, ultrasonicators of 4000W, 10,000W or 16,000W are clusterizable and thereby capable to lyse and homogenize virtually every amount of cellular matter.
Application and Utilization of Ultrasonic Tissue Homogenizers
Ultrasonic homogenizers are commonly used for tissue grinding, cell disruption & lysis and homogenization of biological samples prior to the extraction of intracellular matter in the molecular range. By the controlled sonication of the samples, all steps from lysis to extraction and homogenization can be carried out using the same ultrasonic cell disruptor.
The great advantage of ultrasonic homogenizers lies in the easily adjustable power input and sonication intensity. Hielscher ultrasonic cell disruptors (lysers) allow to control sonication intensity by adjusting the amplitude from 20% to 100%.
Alternatively to the continuous operation, the ultrasonic homogenizer can be set to cycle mode, e.g. for the sonication of heat-sensitive tissue. Sonication in pulse mode means the ultrasonicator generates high intense ultrasonic waves in periodical cycles. The duration of the sonication period and the rest time can be individually set by the user.
At pulsed sonication, during the rest period, the sonicated material can return to its resting state and the sample can cool down (using the rest time for heat dissipation). Thereby, processing intensity can be set accurately in accordance to the tissue and unwanted sample heating is reduced.
Ultrasonic tissue homogenizers are robust and user-friendly whilst producing reliable results assuring reproducibility due to full control over all important process parameters.
Common applications fields of ultrasonic tissue grinding, cell disruption, lysis, extraction and homogenization are found in biological and pharmaceutical laboratories, microbiology, proteomic and genomic research (e.g. protein release before Western blotting and enzyme-linked immunosorbent assay ELISA), but also in the production of botanical extracts and bioactive compounds for the food, pharma and cosmetic industries.
Click here for specific process recommendations concerning sample preparation, tissue homogenization, lysis and extraction (e.g. cells, bacteria, spores, active compounds)!
Literature / References
- Nico Böhmer, Andreas Dautel, Thomas Eisele, Lutz Fischer (2012): Recombinant expression, purification and characterisation of the native glutamate racemase from Lactobacillus plantarum NC8. Protein Expr Purif. 2013 Mar;88(1):54-60.
- Brandy Verhalen, Stefan Ernst, Michael Börsch, Stephan Wilkens (2012): Dynamic Ligand-induced Conformational Rearrangements in P-glycoprotein as Probed by Fluorescence Resonance Energy Transfer Spectroscopy. J Biol Chem. 2012 Jan 6;287(2): 1112-27.
- Claudia Lindemann, Nataliya Lupilova, Alexandra Müller, Bettina Warscheid, Helmut E. Meyer, Katja Kuhlmann, Martin Eisenacher, Lars I. Leichert (2013): Redox Proteomics Uncovers Peroxynitrite-Sensitive Proteins that Help Escherichia coli to Overcome Nitrosative Stress. J Biol Chem. 2013 Jul 5; 288(27): 19698–19714.
- Elahe Motevaseli, Mahdieh Shirzad, Seyed Mohammad Akrami, Azam-Sadat Mousavi, Akbar Mirsalehian, Mohammad Hossein Modarressi (2013): Normal and tumour cervical cells respond differently to vaginal lactobacilli, independent of pH and lactate. ed Microbiol. 2013 Jul; 62(Pt 7):1065-1072.
Facts Worth Knowing
Ultrasonic tissue homogenizers are often referred to as probe sonicator, sonic lyser, ultrasound disruptor, ultrasonic grinder, sono-ruptor, sonifier, sonic dismembrator, cell disrupter, ultrasonic disperser or dissolver. The different terms result from the various applications that can be fulfilled by sonication.