Sterile Homogenization using Sonification
For sterile homogenization, a sample is placed into a sterile container which then is agitated in order to obtain a homogeneous blend. Ultrasonification is a highly efficient and reliable technique to treat one or multiple samples under sterile conditions and results in a rapid sterile homogenization.
Why is Ultrasonification Superior for Sterile Homogenization?
Sterile homogenization is a process used to reduce the size of particles in liquid or liquid-solid samples, usually for the preparation of analyis or testing. It is typically used in the production of pharmaceuticals, food and beverage products, and cosmetics, as well as in scientific research and development.
In the process of sterile homogenization, a sample is placed in a sterile container and then subjected to mechanical force, such as high-performance ultrasonication.
Ultrasonic shear forces – generated by acoustic cavitation – create highly intense mechanical agitation to break down the particles into smaller sizes. After sonication, sample is removed from the sterile container and then filtered to remove any large particles or debris that may have been generated during the homogenization process.
Sterile homogenization is important in many industries because it allows for the production of consistent, uniform products, whilst keeping the sample free from any contamination. This means sterile homogenization using indirect sonification helps to ensure the integrity and purity of the final product.
- use standard sterile disposables
- intense agitation
- uniform and repeatable
- for any sample number and volume
- easy and safe to operate
Powerful Sonication Systems for Sterile Homogenization
Hielscher Ultrasonics offers various solutions for sterile sample homogenization:
The VialTweeter is ideal for the sonication of up to 10 Eppendorf tubes and other vials simultaneously. The closed sample tubes are placed into the VialTweeter sonotrode. Additionally, larger sample vessels can be clamped to the front. The VialTweeter provides a uniformly intense homogenization of all samples.
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The Hielscher ultrasonic cuphorn is a powerful ultrasonic water bath for the simultaneous sonification of larger beakers or up to 5 vials, which are put into the vial holder. Intense ultrasound waves are transmitted through the water bath into the sample vessels and provide a rapid and uniform homogenization.
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The UIP400MTP is a versatile ultrasonic system for the sonication of any standard microtiter plate type as well as simultaneous homogenization of several vials and tubes. Additionally, the UIP400MTP is an potent alternative to flapping homogenizers. Just place your bag, pouch or sachet into the water bath. The UIP400MTP will reliably produce a homogeneous sample.
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The GDmini2 is Hielscher‘s ultrasonic micro-reactor for the continuous homogenization of samples using a disposable pipe or tube made from glass or plastics. The GDmini2 works as an ultrasonic homogenizer agitation a straight glass or plastic tube under sterile conditions. It homogenizes, disperses, disrupts cells or emulsifies liquids that flow through the mounted tube. It can be used as a sterile inline reactor for single-pass or recirculated ultrasonic processing.
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Syringe Agitation System
Sonicate any standard syringe with the Hielscher ultrasonicator for indirect syringe sonication. The syringe is clamped into a fixture of the Hielscher ultrasonic homogenizer. Intense ultrasound waves are transmitted through the syringe walls and coupled into the medium for effective homogenization. The Hielscher syringe sonication system is ideal for dissolving APIs and therapeutics before medical administration.
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Literature / References
- Laughton, Stephanie; Laycock, Adam; von der Kammer, Frank; Hofmann, Thilo; Casman, Elizabeth; Rodrigues, Sónia; Lowry, Gregory (2019): Persistence of copper-based nanoparticle-containing foliar sprays in Lactuca sativa (lettuce) characterized by spICP-MS. Journal of Nanoparticle Research 2019.
- Yang, Yihui; Ahmed, Bilal; Mitchell, Christopher; Quon, Justin; Siddique, Humera; Houson, Ian; Florence, Alastair; Papageorgiou, Charles (2021): Investigation of Wet Milling and Indirect Ultrasound as Means for Controlling Nucleation in the Continuous Crystallization of an Active Pharmaceutical Ingredient. Organic Process Research & Development 25, 9; 2021. 2119–2132.
- Gajek, Ryszard; Barley, Frank; She, Jianwen (2013): Determination of essential and toxic metals in blood by ICP-MS with calibration in synthetic matrix. Analytical Methods 5(9), 2013. 2193-2202.
- Suslick, K.S. (1998): Kirk-Othmer Encyclopedia of Chemical Technology; 4th Ed. J. Wiley & Sons: New York, 1998, vol. 26, 517-541.