Utrasonic topic: "How to facilitate the Sample Preparation "
In daily laboratory work, sample preparation is a procedure in which a sample is treated prior to its analysis. Ultrasonic lab homogenizers are a reliable and powerful tool to prepare samples before common analysis steps such as chromatography (e.g. GC, LC, UPLC, IC), mass spectrometry (e.g. GC/MS, TD GC-MS, LC/MS), microscopy (e.g. SEM, TEM), surface analysis (e.g. SEM, TEM, EDX, XRD, FTIR), elemental analysis techniques etc.
Ultrasoniators fulfil common sample preparation tasks such as homogenising of solids and liquids, emulsifying of two or more immiscible liquids, dispersing powders, milling of nanoparticles, extracting of bioactive compounds or analytes, deaerating and degassing of samples etc. rapidly and reliable.
Since Hielscher ultrasonic devices can precisely controlled, all sonication results are reproducible. Furthermore, all ultrasonically obtained results can be linearly scaled to smaller or larger volumes. This facilitates the reproduction of sample preparation and analytical results significantly.
Hielscher Ultrasonics manufactures various ultrasonic lab homogenizers such as hand-held and stand-mounted devices, the VialTweeter for the simultaneous indirect sonication of up to 10 vials without cross-contamination or the SonoStep, a sample prep unit which combines sonication, stirring and pumping in one compact system. Digital touch-screen, integrated SD for automatic data protocoling and sample illumination ensure user-friendliness and comfort.
Read more about how ultrasonic sample preparation can facilitate your daily lab work!
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Assays such as ELISA are widely used for in-vitro diagnostics, disease-related protein detection and quality control (e.g. monitoring food allergens). Ultrasonic sample preparation is a rapid, reliable and reproducible technique to lyse cell and isolate intracellular proteins, DNA, RNA…https://www.hielscher.com/ultrasonic-sample-prep-for-elisa-assays.htm
Sample preparation before analysis can require various pre-analytical processes such as tissue homogenization, lysis, extraction of proteins, DNA, RNA, organelles and other intracellular substances, dissolving and degassing. The VialTweeter is a unique ultrasonic device that prepares multiple sample tubes…https://www.hielscher.com/sample-preparation-with-the-ultrasonic-vialtweeter.htm
Whilst degassing or outgassing is often an extremely time-consuming process step, ultrasonication can accelerate the coalescence of gas bubbles and their rise significantly. Ultrasonic outgassing can be used in batch and inline setups and can be also combined with…https://www.hielscher.com/highly-efficient-de-aeration-of-liquids-using-ultrasonics.htm
Protein extraction is an essential sample preparation step in proteomics. Proteins can be extracted from plant and animal tissue, yeasts and microorganisms. Sonication is a reliable, efficient protein extraction method giving high protein yields within a short extraction time.…https://www.hielscher.com/ultrasonic-protein-extraction-from-tissue-and-cell-cultures.htm
With the UP400St (400W, 24kHz), the serial of Hielscher’s digital ultrasonic devices is expanded by a powerful 400 watts ultrasonicator. The new UP400St is the successor of UP400S - one of Hielscher's most popular lab systems. Its high ultrasound…https://www.hielscher.com/up400st-powerful-ultrasonicator.htm
Ultrasonic extraction is a green, environmental-friendly method of extraction that can be applied to small lab samples as well as for the extraction of valuable compounds on commercial production scale. The United State Environmental Protection Agency (EPA) recommends a…https://www.hielscher.com/epa3550-ultrasonic-extraction-guide.htm
The GDmini2 is an ultrasonic micro-reactor for the indirect, temperature-controlled sonication of liquid media. Applications include: Homogenization, emulsification, particle synthesis, solvent extraction, cell lysis and fragmentation. The GDmini2 is an ultrasonic homogenizer in the shape of a straight glass…https://www.hielscher.com/gdmini2-ultrasonic-inline-micro-reactor.htm
Ultrasonic lab homogenizers allow for fast and efficient emulsifying and dispersing of cremes, lotions, ointments, suspensions and tinctures. Power ultrasound is a reliable compounding technique for high quality pharmaceuticals Due to intense cavitational shear forces, ultrasonic mixing results in…https://www.hielscher.com/ultrasonic-mixing-technology-for-pharmacies.htm
The 200 watts ultrasonic device UP200St_TD offers a broad versatility as this basic unit allows for three completely different setups. The basic system consists in the top-down transducer UP200St_TD that is driven by the 200 watt generator (UP200St-G) suppling…https://www.hielscher.com/all-in-one-sample-preparation-unit-up200st_td.htm
Hielscher Ultrasonics offers you a broad product range of ultrasonic accessories for the direct and indirect sonication of small lab samples to full commercial production. Find below an overview over our sonotrodes, flow-through reactors and accessories for both, direct…https://www.hielscher.com/ultrasonic-sonotrodes-flow-cells-accessories.htm
Ultrasonication is a safe & efficient method of tissue homogenization. Ultrasonic applications include the preparation tissue and emulsions, the extraction of proteins, DNA/ RNA and active compounds, or the activation/ inactivation of enzymes and yeast. Precise controllability of the…https://www.hielscher.com/ultrasonic-tissue-homogenizers.htm
Before purification or characterization of intracellular macromolecules such as proteins, organelles, enzymes or active compounds, an efficient method for tissue lysis and cell disintegration is required. Ultrasonication is a effective method for cell preparation that releases the intracellular material…https://www.hielscher.com/how-to-use-hielschers-ultrasonic-tissue-homogenizers.htm