Utrasonic topic: "How to facilitate the Sample Preparation "
In daily laboratory work, sample preparation is a procedure in which a sample is treated prior to its analysis. Ultrasonic lab homogenizers are a reliable and powerful tool to prepare samples before common analysis steps such as chromatography (e.g. GC, LC, UPLC, IC), mass spectrometry (e.g. GC/MS, TD GC-MS, LC/MS), microscopy (e.g. SEM, TEM), surface analysis (e.g. SEM, TEM, EDX, XRD, FTIR), elemental analysis techniques etc.
Ultrasoniators fulfil common sample preparation tasks such as homogenising of solids and liquids, emulsifying of two or more immiscible liquids, dispersing powders, milling of nanoparticles, extracting of bioactive compounds or analytes, deaerating and degassing of samples etc. rapidly and reliable.
Since Hielscher ultrasonic devices can precisely controlled, all sonication results are reproducible. Furthermore, all ultrasonically obtained results can be linearly scaled to smaller or larger volumes. This facilitates the reproduction of sample preparation and analytical results significantly.
Hielscher Ultrasonics manufactures various ultrasonic lab homogenizers such as hand-held and stand-mounted devices, the VialTweeter for the simultaneous indirect sonication of up to 10 vials without cross-contamination or the SonoStep, a sample prep unit which combines sonication, stirring and pumping in one compact system. Digital touch-screen, integrated SD for automatic data protocoling and sample illumination ensure user-friendliness and comfort.
Read more about how ultrasonic sample preparation can facilitate your daily lab work!
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Do you want to master the science of cell lysis? Look no further! In this step-by-step guide, we will walk you through the process of ultrasonic cell disruption and ensure that your cell lysis technique gets you optimal results. Whether…https://www.hielscher.com/ultrasonic-lysis-step-by-step-guide-to-perfecting-cell-disruption.htm
For sterile homogenization, a sample is placed into a sterile container which then is agitated in order to obtain a homogeneous blend. Ultrasonification is a highly efficient and reliable technique to treat one or multiple samples under sterile conditions and…https://www.hielscher.com/sterile-homogenization-using-sonification.htm
Ultrasonication is an important processing method for the isolation of the monkeypox virus (MPXV) from analytical samples, for virus DNA fragmentation as well as in monkeypox vaccine manufacturing. For sample preparation before diagnostics and analysis (PCR, ELISA etc.) ultrasonication is…https://www.hielscher.com/ultrasonic-applications-in-monkeypox-virus.htm
Ultrasonically agitated syringe filters enable to work at higher flow rates and loading capacities. Samples with higher solid contents can be reliable and efficiently processed using an ultrasonically vibrated syringe filters. This allows for higher throughputs. Ultrasonically agitation of syringe…https://www.hielscher.com/ultrasonically-agitated-syringe-filter-for-higher-throughputs.htm
Next Generation Sequencing (NGS) requires the reliable shearing and fragmentation of genomic DNA in order to sequence genomic DNA strands and to create genome libraries. The controlled fragmentation of DNA into DNA fragments is an essential sample preparation step required…https://www.hielscher.com/ultrasonic-dna-fragmentation-for-next-gen-sequencing.htm
Ultrasonic lab homogenizers can be used for batch and inline processing of liquids and slurries. Typical applications include homogenization, dispersing, emulsification, dissolving as well as sonochemical reactions. For the continuous inline sonication, flow cells and in-line reactors in various sizes…https://www.hielscher.com/flow-cells-and-inline-reactors-for-lab-ultrasonicators.htm
Assays such as ELISA are widely used for in-vitro diagnostics, disease-related protein detection and quality control (e.g. monitoring food allergens). Ultrasonic sample preparation is a rapid, reliable and reproducible technique to lyse cell and isolate intracellular proteins, DNA, RNA and…https://www.hielscher.com/ultrasonic-sample-prep-for-elisa-assays.htm
Sample preparation before analysis can require various pre-analytical processes such as tissue homogenization, lysis, extraction of proteins, DNA, RNA, organelles and other intracellular substances, dissolving and degassing. The VialTweeter is a unique ultrasonic device that prepares multiple sample tubes simultaneously…https://www.hielscher.com/sample-preparation-with-the-ultrasonic-vialtweeter.htm
Whilst degassing or outgassing is often an extremely time-consuming process step, ultrasonication can accelerate the coalescence of gas bubbles and their rise significantly. Ultrasonic outgassing can be used in batch and inline setups and can be also combined with conventional…https://www.hielscher.com/highly-efficient-de-aeration-of-liquids-using-ultrasonics.htm
Protein extraction is an essential sample preparation step in proteomics. Proteins can be extracted from plant and animal tissue, yeasts and microorganisms. Sonication is a reliable, efficient protein extraction method giving high protein yields within a short extraction time. Protein…https://www.hielscher.com/ultrasonic-protein-extraction-from-tissue-and-cell-cultures.htm
With the UP400St (400W, 24kHz), the serial of Hielscher digital sonicators is expanded by a powerful 400 watts ultrasonic probe-type homogenizer. The new UP400St is the successor of UP400S - one of the most popular lab sonicators. Its high ultrasound…https://www.hielscher.com/up400st-powerful-ultrasonicator.htm
Ultrasonic extraction is a green, environmental-friendly method of extraction that can be applied to small lab samples as well as for the extraction of valuable compounds on commercial production scale. The United State Environmental Protection Agency (EPA) recommends a variety…https://www.hielscher.com/epa3550-ultrasonic-extraction-guide.htm