Utrasonic topic: "Ultrasonic Cell Disruption"
Cell disruption is a technique or process used to release and isolate biological molecules from inside a cell. Ultrasonication is a highly efficient method to perforate and disrupt cell walls and membranes so that the intracellular material and the targeted biomolecules are released into the solvent. As a non-thermal, mild , yet highly efficient technique, ultrasonic disruptors are used in lab and industry to lyse cells and to produce superior extracts. Ultrasonic cell disruptors are used to prepare DNA and RNA as well as to extract bioactive compounds such as vitamins, polyphenols or natural pigments.
Read more about the manifold applications of ultrasonic cell disruption and extraction!
12 pages about this topic are being shown:
Next Generation Sequencing (NGS) requires the reliable shearing and fragmentation of genomic DNA in order to sequence genomic DNA strands and to create genome libraries. The controlled fragmentation of DNA into DNA fragments is an essential sample preparation step…https://www.hielscher.com/ultrasonic-dna-fragmentation-for-next-gen-sequencing.htm
Algae, macro- and microalgae, contain many valuable compounds, which are used as nutritional foods, food additives or as fuel or fuel feedstock. In order to release the target substances from the algae cell a potent and efficient cell disruption…https://www.hielscher.com/ultrasonication-to-improve-algae-cell-disruption-and-extraction.htm
Drosophila melanogaster is widely used in laboratories as model organism. Therefore, pre-analytical preparation steps such as lysis, cell disruption, protein extraction and DNA shearing of Drosophila melanogaster samples must be frequently carried out. Ultrasonic dismembrators are reliable and efficient…https://www.hielscher.com/lysis-of-drosophila-melanogaster-samples.htm
Microtiter plates, multi-well plates, 96-well plates or ELISA plates are used for the mass sample cultivation and processing of biological samples. With the microtiter plate ultrasonicator UIP400MTP, Hielscher offers a unique ultrasonic solution of the uniform and reliable sample…https://www.hielscher.com/microtiter-plate-mass-sample-preparation-by-ultrasonication.htm
C. elegans, a nematode worm, is a widely used model organism in biology. Sample preparation before analysis requires lysis, protein and lipid extraction as well as RNA fragmentation, which can be reliably performed via sonication. Ultrasonic cell disruptors are…https://www.hielscher.com/ultrasonic-preparation-of-c-elegans-samples.htm
BL21 cells are a strain of E. coli that is widely used in research laboratories, biotechnology and industrial production due to their capability to express proteins highly efficient. Ultrasonic cell disruption, lysis and protein extraction is the common method…https://www.hielscher.com/cell-lysis-of-bl21-cells-by-ultrasonication.htm
Assays such as ELISA are widely used for in-vitro diagnostics, disease-related protein detection and quality control (e.g. monitoring food allergens). Ultrasonic sample preparation is a rapid, reliable and reproducible technique to lyse cell and isolate intracellular proteins, DNA, RNA…https://www.hielscher.com/ultrasonic-sample-prep-for-elisa-assays.htm
The Hielscher VialTweeter is a unique ultrasonic multi-sample preparation unit, which is used to inactivate the coronavirus SARS-COV-2. The VialTweeter allow to prepare up to 10 sample vials simultaneously and is thereby the ideal unit for mass sample processing.…https://www.hielscher.com/protocol-for-sars-cov-2-coronavirus-inactivation-with-sonication.htm
Ultrasonic dismembrators are commonly used in the laboratory, bench-top and industry to prepare homogeneous colloidal suspensions such as emulsions and dispersions with droplet or particle sizes in the submicron- and nano-range. Furthermore, ultrasonic dismembrators are used for cell disruption,…https://www.hielscher.com/most-efficient-ultrasonic-dismembrators.htm
Mycoprotein are fungus-derived protein produced for human consumption, which are mainly used to prepare meat substitutes or “fake meat”. Ultrasonic extraction and homogenization is a highly efficient method to release mycoprotein from the fungus achieving very high protein yields…https://www.hielscher.com/ultrasonic-extraction-of-mycoprotein.htm
Ultrasonic extraction enhances the yield of extracted sucrose from sugar beet cossettes and reduces the extraction process duration significantly. Sonication is a simple and safe technique, which can be easily combined with the current counter-current flow extraction technology in…https://www.hielscher.com/ultrasonic-extraction-of-sugar-from-sugar-beet-cossettes.htm
Ultrasonic extraction is the superior method to disrupt algae cells effectively and rapidly. Sonication can release the complete amount of the bioactive compounds, which makes the ultrasonic technique highly efficient. How To Extract Proteins, Lipids and Phenolics from Algae…https://www.hielscher.com/ultrasonic-algae-extraction-for-nutritional-supplements.htm