Utrasonic topic: "Ultrasonic Cell Disruption"
Cell disruption is a technique or process used to release and isolate biological molecules from inside a cell. Ultrasonication is a highly efficient method to perforate and disrupt cell walls and membranes so that the intracellular material and the targeted biomolecules are released into the solvent. As a non-thermal, mild , yet highly efficient technique, ultrasonic disruptors are used in lab and industry to lyse cells and to produce superior extracts. Ultrasonic cell disruptors are used to prepare DNA and RNA as well as to extract bioactive compounds such as vitamins, polyphenols or natural pigments.
Read more about the manifold applications of ultrasonic cell disruption and extraction!
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Ultrasonication Outcompetes Other Extraction Methods by Speed
The rapid procedure of ultrasonic extraction and the therefrom resulting time savings in extract production are one of many advantages of ultrasonic extraction of bioactive compounds from plants. Ultrasonic extraction has been scientifically compared to alternative extraction techniques such as…
https://www.hielscher.com/ultrasonication-outcompetes-other-extraction-methods-by-speed.htm
Ultrasonic Lysis of Bioengineered Cells in Industrial Production
Bioengineered bacteria species such E. coli as well as genetically modified mammalian and plant cell types are used widely in biotech to express molecules. In order to release these synthesized bio-molecules, a reliable cell disruption technique is required. High-performance ultrasonication…
https://www.hielscher.com/ultrasonic-lysis-of-bioengineered-cells-in-industrial-production.htm
Ultrasonic DNA Fragmentation for Next Gen Sequencing
Next Generation Sequencing (NGS) requires the reliable shearing and fragmentation of genomic DNA in order to sequence genomic DNA strands and to create genome libraries. The controlled fragmentation of DNA into DNA fragments is an essential sample preparation step required…
https://www.hielscher.com/ultrasonic-dna-fragmentation-for-next-gen-sequencing.htm
Ultrasonication to Improve Algae Cell Disruption and Extraction
Algae, macro- and microalgae, contain many valuable compounds, which are used as nutritional foods, food additives or as fuel or fuel feedstock. In order to release the target substances from the algae cell a potent and efficient cell disruption technique…
https://www.hielscher.com/ultrasonication-to-improve-algae-cell-disruption-and-extraction.htm
Ultrasonic Lysis of Drosophila melanogaster Samples
Drosophila melanogaster is widely used in laboratories as model organism. Therefore, pre-analytical preparation steps such as lysis, cell disruption, protein extraction and DNA shearing of Drosophila melanogaster samples must be frequently carried out. Ultrasonic dismembrators are reliable and efficient and…
https://www.hielscher.com/lysis-of-drosophila-melanogaster-samples.htm
Microtiter Plate Mass Sample Preparation by Ultrasonication
Microtiter plates, multi-well plates, 96-well plates or ELISA plates are used for the mass sample cultivation and processing of biological samples. With the microtiter plate ultrasonicator UIP400MTP, Hielscher offers a unique ultrasonic solution of the uniform and reliable sample preparation…
https://www.hielscher.com/microtiter-plate-mass-sample-preparation-by-ultrasonication.htm
Ultrasonic Preparation of C. Elegans Samples
C. elegans, a nematode worm, is a widely used model organism in biology. Sample preparation before analysis requires lysis, protein and lipid extraction as well as RNA fragmentation, which can be reliably performed via sonication. Ultrasonic cell disruptors are reliable,…
https://www.hielscher.com/ultrasonic-preparation-of-c-elegans-samples.htm
Cell Lysis of BL21 Cells by Ultrasonication
BL21 cells are a strain of E. coli that is widely used in research laboratories, biotechnology and industrial production due to their capability to express proteins highly efficient. Ultrasonic cell disruption, lysis and protein extraction is the common method to…
https://www.hielscher.com/cell-lysis-of-bl21-cells-by-ultrasonication.htm
Ultrasonic Sample Prep for ELISA Assays
Assays such as ELISA are widely used for in-vitro diagnostics, disease-related protein detection and quality control (e.g. monitoring food allergens). Ultrasonic sample preparation is a rapid, reliable and reproducible technique to lyse cell and isolate intracellular proteins, DNA, RNA and…
https://www.hielscher.com/ultrasonic-sample-prep-for-elisa-assays.htm
Protocol for SARS-CoV-2 Coronavirus Inactivation with Sonication
The Hielscher VialTweeter is a unique ultrasonic multi-sample preparation unit, which is used to inactivate the coronavirus SARS-COV-2. The VialTweeter allow to prepare up to 10 sample vials simultaneously and is thereby the ideal unit for mass sample processing. Inactivation…
https://www.hielscher.com/protocol-for-sars-cov-2-coronavirus-inactivation-with-sonication.htm
Most Efficient Ultrasonic Dismembrators
Ultrasonic dismembrators are commonly used in the laboratory, bench-top and industry to prepare homogeneous colloidal suspensions such as emulsions and dispersions with droplet or particle sizes in the submicron- and nano-range. Furthermore, ultrasonic dismembrators are used for cell disruption, lysis,…
https://www.hielscher.com/most-efficient-ultrasonic-dismembrators.htm
Ultrasonic Extraction of Mycoprotein
Mycoprotein are fungus-derived protein produced for human consumption, which are mainly used to prepare meat substitutes or “fake meat”. Ultrasonic extraction and homogenization is a highly efficient method to release mycoprotein from the fungus achieving very high protein yields in…
https://www.hielscher.com/ultrasonic-extraction-of-mycoprotein.htm