Utrasonic topic: "cell disruption"
Cell disruption is a technique or process used to release and isolate biological molecules from inside a cell. Ultrasonication is a highly efficient method to perforate and disrupt cell walls and membranes so that the intracellular material and the targeted biomolecules are released into the solvent. As a non-thermal, mild , yet highly efficient technique, ultrasonic disruptors are used in lab and industry to lyse cells and to produce superior extracts. Ultrasonic cell disruptors are used to prepare DNA and RNA as well as to extract bioactive compounds such as vitamins, polyphenols or natural pigments.
Read more about the manifold applications of ultrasonic cell disruption and extraction!
24 pages about this topic are being shown:
Ultrasonic Capsaicin Extraction from Hot Chili Peppers
Capsaicin is the main flavour and spice compound in hot peppers, also known as chilies. To produce high-quality capsaicin as aromatic flavour and medicinal ingredient, an efficient, yet mild extraction technique is required to prevent degradation. Ultrasonic extraction is…
https://www.hielscher.com/ultrasonic-capsaicin-extraction-from-hot-chili-peppers.htm
Ultrasonic Astaxanthin Extraction for Higher Yields
Astaxanthin is a highly potent antioxidant used in pharmaceuticals and nutritional supplements. In order to produce high-quality astaxanthin from natural sources such as algae, a high-performance extraction technique is required. Ultrasonic extraction is a mechanical treatment, which gives high…
https://www.hielscher.com/ultrasonic-astaxanthin-extraction-for-higher-yields-and-superior-quality.htm
Ultrasonic Vanilla Extraction – A Non-Thermal Method
Vanilla extract is a flavour solution extractes from vanilla pods in a solution of ethanol and water. To produce high-quality vanillin as aroma, flavour and fragrance component, an efficient, yet mild extraction technique is required to prevent decomposition. Ultrasonic…
https://www.hielscher.com/ultrasonic-vanilla-extraction-a-non-thermal-method.htm
Ultrasonic Quercetin Extraction
Quercetin is a plant compound of the group of polyphenols, which is known for manifold health beenfits. To produce high-quality quercetin for food additives and supplements, an efficient, yet mild extraction technique is required to prevent decomposition. Ultrasonic extraction…
https://www.hielscher.com/ultrasonic-quercetin-extraction.htm
Ultrasonic Extraction of Bioactive Compounds from Bitter Melon
Bitter melons (also known as bitter apple, bitter gourd, bitter squash, balsam-pear) is a fruit high in health-beneficial compounds such as antioxidants, flavonoids, phytosterols, and saponins. Sonication is well known to intensify the extraction of bioactive substances from botanicals.…
https://www.hielscher.com/ultrasonic-extraction-of-bioactive-compounds-from-bitter-melon.htm
Ultrasonic Cold-Water Extraction of Mogrosides from Monk Fruit
Monk fruit (S. grosvenorii) extract and particularly its compound mogroside V is used as a natural non-caloric sweetener. Mogrosides. Ultrasonic extraction is a simple, rapid, reproducible, and reliable extraction method successfully applied to produce natural non-caloric sweetener from monk…
https://www.hielscher.com/ultrasonic-cold-water-extraction-of-mogrosides-from-monk-fruit.htm
Ultrasonic Extraction of Medicinal Herbs
Herbs, spices and other plant materials are rich in phytochemicals, which are valued components for medicinal and nutritional products. Sonication breaks the cell structure and releases the bioactive compounds - resulting in higher yields and faster extraction rates. As…
https://www.hielscher.com/ultrasonic-extraction-of-medicinal-herbs.htm
Ultrasonic Cucurmin Extraction
Curcumin is a pharmacological and nutritional phytochemical present in the rhizomes of Curcuma longa. For high extraction yield, sonication is the most efficient technique. Ultrasonic extraction results in high quality, high yield and requires only short processing time. Ultrasonic…
https://www.hielscher.com/ultrasonic-cucurmin-extraction.htm
Solvents for Ultrasonic Extraction from Plants
Ultrasonic extraction features many advantages such as high yields, fast extraction rates, environmental-friendliness and low energy consumption. One of the strongest benefits is the use of water as extraction medium. However, sonication can be used with manifold solvent systems…
https://www.hielscher.com/solvents-for-ultrasonic-extraction-from-plants.htm
Ultrasonic DNA Shearing
During DNA and RNA shearing, DNA molecules are broken into smaller pieces. DNA / RNA fragmentation is one of the important sample prep steps required create libraries for next generation sequencing (NGS). Ultrasonic DNA shearing uses the forces of…
https://www.hielscher.com/ultrasonic-dna-shearing.htm
Ultrasonic Lysis of E. Coli
E. coli bacteria are the most commonly used bacteria in microbiology and biotechnology. Ultrasonic cell disruptors deliver reliable and reproducible results for the lysis of E. coli. Intense yet precisely controllable cavitation and shear forces result in complete disruption…
https://www.hielscher.com/ultrasonic-lysis-of-e-coli.htm
EPA3550 Ultrasonic Extraction Guide
Ultrasonic extraction is a green, environmental-friendly method of extraction that can be applied to small lab samples as well as for the extraction of valuable compounds on commercial production scale. The United State Environmental Protection Agency (EPA) recommends a…
https://www.hielscher.com/epa3550-ultrasonic-extraction-guide.htm
Power-Ultrasound for Advanced Microbial Stability of Paints
Microbial deterioration can damage both water- and solvent-borne paint formulations. Environmental regulations restrict the use of biocides. Sonication is an environmental-friendly, effective method of paint preservation. Power ultrasound allows even for the recycling of microbially spoilt paints and coatings.…
https://www.hielscher.com/power-ultrasound-for-advanced-microbial-stability-of-paints.htm
Ultrasonic Lysis for Western Blotting
The western blot is an analytical procedure for the detection of specific proteins in a sample of tissue homogenate or cell extract. To run a Western blot or to measure enzyme activity, many assays require access to the materials…
https://www.hielscher.com/ultrasonic-lysis-for-western-blotting.htm
GDmini2 – Ultrasonic Inline Micro-Reactor
The GDmini2 is an ultrasonic micro-reactor for the indirect, temperature-controlled sonication of liquid media. Applications include: Homogenization, emulsification, particle synthesis, solvent extraction, cell lysis and fragmentation. The GDmini2 is an ultrasonic homogenizer in the shape of a straight glass tube.…
https://www.hielscher.com/gdmini2-ultrasonic-inline-micro-reactor.htm
Closed Ultrasonic Reactor for Pharmaceutical Batch Processing
Many processes as mixing, dispersing and extraction of high-value materials are carried out as batch process. Hielscher's closed ultrasonic reactor is ideal for an integrated ultrasonic processing under closed and precisely controlled conditions. Hielscher's closed ultrasonic reactor is perfectly…
https://www.hielscher.com/closed-ultrasonic-reactor-for-pharmaceutical-batch-processing.htm
Ultrasonically Assisted Catalytic Extraction
Hielscher ultrasonic reactors are used in many industries to assist and improve the catalytic extraction processing (CEP) or so-called phase-transfer extraction (PTE). Catalytic extraction involves a heterogenous immiscible phase system, such as liquid-liquid or liquid-solid. Ultrasonic high shear and…
https://www.hielscher.com/ultrasonically-assisted-catalytic-extraction.htm
Ultrasonic Tissue Homogenizers
Ultrasonication is a safe and efficient method of tissue homogenization. Ultrasonic applications include the preparation tissue and emulsions, the extraction of proteins, DNA/ RNA and active compounds, or the activation/ inactivation of enzymes and yeast. Precise controllability of the ultrasound…
https://www.hielscher.com/ultrasonic-tissue-homogenizers.htm
Sonication of Wine – Innovative Applications of Ultrasound in Wineries
Ultrasound is a non-thermal processing method, which is already widely used in the food industry due to its mild application but significant effects on the product. For the wineries, sonication offers various applications such as the extraction of flavours,…
https://www.hielscher.com/sonication-of-wine-innovative-applications-of-ultrasound-in-wineries.htm
Ultrasonic Lysis: Cell Disruption & Extraction
Cell disintegration or lysis is a common part of daily sample preparation in biotech laboratories. The goal of lysis is to disrupt parts of the cell wall or the complete cell to release biological molecules. The so-called lysate can…
https://www.hielscher.com/ultrasonic-lysis-cell-disruption-extraction.htm
Ultrasonically Assisted Saffron Extraction
Ultrasound is well known for its extraction capacity. The ultrasonically assisted extraction of active compounds, flavours and spices from plant material is a very successful application as ultrasonically generated cavitation disruptes plant cells and enhances mass transfer so that…
https://www.hielscher.com/ultrasonically-assisted-saffron-extraction.htm
Power Ultrasound for the Improvement of Juices & Smoothies
Researches from the Technical University Berlin found that ultrasonication is a very effective processing method to improve fruit and vegetable juices as well as smoothies. Being a non-thermal food process technique, ultrasound provides a mild but effective treatment that…
https://www.hielscher.com/power-ultrasound-for-the-improvement-of-juices-smoothies.htm
Algae Grow Lab
Algae Cultivation Algae Grow Lab developed a series of tubular and flat photobioreactors for algae cultivation as well as a process of cell ultrasonic destruction based on Hielscher ultrasonic processors equipped with flow cells. The general flow diagram of…
https://www.hielscher.com/algae-grow-lab.htm
Ultrasonic Extraction and Preservation
The disintegration of cell structures (lysis) by means of ultrasound is used for the extraction of intra-cellular compounds or for the microbial inactivation. Background In microbiology, ultrasound is primarily associated with cell disruption (lysis) or disintegration (Allinger…
https://www.hielscher.com/extraction_01.htm