Video: Mushroom Extraction – Ultrasonic Bath vs Probe Sonicator

This video compares the extraction efficiency of an ultrasonic bath and the Hielscher UP100H ultrasonic probe. Both methods are tested using crushed Chaga mushrooms in a water-ethanol mixture to extract bioactive compounds like betulin and phytosterols.
Non-thermal extraction at -10°C preserves heat-sensitive components, enhancing extract quality. The UP100H sonicator operates at 100 watts, producing stronger cavitation than the ultrasonic bath, which operates at 70 watts. After processing, the UP100H extract is visibly darker, indicating higher extraction efficiency. Ultraviolet-visible spectroscopy confirms the UP100H’s superior performance. Its extraction curve is significantly higher across the absorption spectrum (300–800 nm), with a notable yield increase in the 340–440 nm range. The probe sonicator’s intense cavitation extracts both water-soluble and water-insoluble components, outperforming the ultrasonic bath in quality and yield. This result aligns with scientific studies showing the efficacy of probe sonicators over bath sonicators for extracting bioactive compounds.