Ultrasonically Agitated Syringe Filter for Higher Throughputs

Ultrasonically agitated syringe filters enable to work at higher flow rates and loading capacities. Samples with higher solid contents can be reliable and efficiently processed using an ultrasonically vibrated syringe filters. This allows for higher throughputs. Ultrasonically agitation of syringe filters can be applied to a variety of membrane types and pore sizes matching your process requirements.

Syringe Filters – Sonicated for Higher Filtration Efficiency

The membrane of a syringe filter is the crucial part that functions as the actual filter and are available in various sizes (diameter), pore sizes and made from different materials such as polyamide (PA), polytetrafluoroethylene (PTFE), polyvinylidene fluoride (PVDF), cellulose acetate (CA), regenerated cellulose (RC), polypropylene (PP), cellulose mixed ester (CME) and polyether sulfone (PES).
When biological samples are treated, the different intensity of adsorption of proteins by different filter materials should be considered. Many syringe filters are available in both non-sterile and sterile versions. Filtration using a syringe filter is often a slow, time consuming process, which is also known for sample loss due to dead volume and incomplete filtration. Ultrasonic agitation of syringe filters makes the filtration process significantly more efficient – resulting in faster and more complete filtration results.

Information Request




Note our privacy policy.


Ultrasonic solution for high-efficiency throughputs in syringe filters using ultrasonic agitation. The picture shows the ultrasonicator UP200St with sonotrode S26d26spec for the sonication of syringe filters.

Ultrasonicator UP200St (200W, 26kHz) with sonotrode S26s26spec for the agitation of syringe filters. The ultrasonic agitation enables for high throughputs in syringe filters.

Ultrasonically agitated syringe filters work with filters of various pore sizes. The pore size of the membrane is an important factor, which determines the purity of the liquid after filtration. The most frequently used syringe filters have a pore size of 0.45 µm or 0.2 µm. A clear filtration with a pore size of 0.45 µm is suitable for a measurement with high-performance liquid chromatography (HPLC) in order to prevent the separation column from clogging with particles. For UHPLC, however, a finer pore size of 0.2 µm makes sense.
In laboratories, where microbiological samples or cell cultures are processed and filters, syringe filters are used to remove bacteria from a solution with thermolabile ingredients (e.g. vitamins or antibiotics in the culture medium). Since these samples cannot be steam-sterilized in an autoclave at 121°C, filtration with a syringe filter is the most commonly used technique of separation. For sterile filtration of a volume of up to 100 ml, syringe filters with a pore size of 0.2 µm or 0.45 µm are most commonly used; however, viruses and mycoplasmas are not retained with either pore size. The so-called “Bacteria Challenge Test” is an assessment that provides information on which types of bacteria are retained with a syringe filter of a certain pore size.
Filters with a pore size of 5 µm are used for pre-filtration in order to remove fractions containing larger particles. Pre-filtration is particularly helpful when a high proportion of solids is present in the sample, which would immediately clog a fine filter membrane.

Hielscher Ultrasonics offers a facile and reliable solution to enhance the filtration efficiency of your syringe filter!

Ultrasonically Improved Syringe Filters for

  • sample preparation before analysis
  • HPLC
  • UHPLC
  • microbiological samples
  • cell suspensions, cell cultures
  • pre-filtration

Hielscher Ultrasonics’ Solution for Improved Syringe Filtration

Ultrasonically agitated syringe filters are highly efficient in removing particulate impurities from liquid samples.
The sonotrode S26d26spec works with the ultrasonic processor UP200St and is readily available for sale. Maximum power coupling at 100% amplitude approx. 40 Watts to the filter. Cavitation noise is audible, when the filter is filed with water. Attention: Basic laws of thermodynamics apply. All power will finally heat the filter and its content. At up to 40W that creates a measurable increase in temperature. In order to avoid damage of the sample and syringe filter, lower amplitudes and pulse operation (short intense burst followed by several seconds of idle time for heat dissipation) are recommended.
The ultrasonic sonotrode can be easily adapted and customized to specific syringe filter types.
Special sonotrode for the simultaneous agitation of several syringe filters are available, too.

Contact Us! / Ask Us!

Ask for more information

Please use the form below to request additional information about the ultrasonic processor for agitation of syringe filters and its price. We will be glad to discuss your application for sample preparation with you and to offer you an ultrasonic system meeting your requirements!









Please note our privacy policy.


Ultrasonic processor UP200St, a 200 watts powerful ultrasonicator for sample preparation applications such as ultrasonic vibrating of syringe filters for improved throughput.

The ultrasonicator UP200St (200W, 26kHz) consists in an ultrasonic transducer and generator. The UP200St ultrasonic system is used successfully for the agitation of syringe filters resulting in an enhanced filtration efficiency.



Literature / References


High performance ultrasonics! Hielscher's product range covers the full spectrum from the compact lab ultrasonicator over bench-top units to full-industrial ultrasonic systems.

Hielscher Ultrasonics manufactures high-performance ultrasonic homogenizers from lab to industrial size.