Flower Extraction – Mild Processing, High Yields by Sonication
Whether you want to make an absolute, flower-infused water or blossom hydrolat, sonication helps you to increase the yield of your flower extract. Learn why sonication is the ideal extraction technique to isolate the delicate biomolecules and essential oils from flowers and petals.
Sonication as Ideal Extraction Technique for Biomolecules and Essential Oils from Flowers and Blossoms
As flowers contain many fragrant, flavorful and therapeutically active compounds, which can be used as ingredients for medicinal products, food, cosmetics and nutritional supplements, they are a highly coveted raw material for potent extracts. Ultrasonic extraction is a potent technique to isolate the desired substances from blossoms. Hence, flowers are a very delicate part of a plant and require accordingly careful treatment. Here come the mild extraction conditions of ultrasonication to the fore. As non-thermal, precisely controllable extra method, sonication produces high yields of flower extracts. Outstanding extract quality and high efficiency are key characteristics of ultrasonic botanical extraction.
Rose Water by Sonication – A Step-by-Step Instruction
To prepare rose water as demonstrated in the video clip above, follow the instruction described here:
- To begin, carefully select fresh roses (Rosa damascena) and remove the petals from the flower pistil. Cut the petals into smaller pieces in order to increase the surface area.
- Since we want to make rose water, we use purified water as solvent.
- Place the petals into a glass beaker and add water. In the video we use the large 10 petals and 200mL water.
- UP200Ht 초음파 발생기 설정 : sonotrode S26d14를 단단히 장착하십시오. 꽃잎은 매우 섬세한 식물 부분이기 때문에 초음파 추출 절차 중에 모든 생체 분자가 보존되도록 진폭을 70%로 조정합니다.
- Immerse the probe into the glass beaker and turn the sonicator on. Sonicate for approx. 90 seconds.
- After sonication, filter the mixture to remove solid residues, obtaining clear and fragrant rose water.
- Collect the rose water in sterile containers for storage and store it in a cool, dark place to maintain its quality and extend its shelf life.
- Use the rose water for culinary or cosmetic applications.
Ultrasonic Blossom Extraction
- rose
- jasmine
- dandelion
- cherry blossom
- lilac
- lavender
- elderflower
- malve
- sage
- chamomile
- magnolia
- daisies
- chestnut blossom
- passionflower
- orange blossom
- calendula/ marigold
- echinacea/ coneflower
- Black locust / robinia
- sow thistle
- ribwort
Essential Oil Extraction using Sonication
Ultrasonically-assisted hydrodistillation is an advanced extraction technique that combines traditional hydrodistillation with ultrasound technology. This method utilizes ultrasonic waves to create cavitation, forming small vapor-filled bubbles that implode, generating microjets and turbulence. These effects enhance particle breakdown, surface cracking, and mixing, thereby significantly improving mass transfer rates.
The process also incorporates bulk heating, which accelerates the vaporization of water and enhances heat transfer. This combined approach reduces extraction time, energy consumption, and solvent use, making ultrasonically-assisted hydrodistillation more efficient and environmentally-friendly compared to conventional methods.
Key benefits of ultrasonically-assisted hydrodistillation include:
- Higher Extraction Efficiency: Enhanced release of essential oils and valuable bioactive compounds.
- Reduced Time and Energy: Shorter extraction times and lower energy consumption.
- 품질 향상: Preservation of compound integrity due to gentle processing conditions.
- Operational Advantages: Safe operation, lower startup time, reduced equipment size, and fewer processing steps.
위의 비교 SEM 이미지는 수소 증류 (왼쪽)와 초음파 보조 수소 증류 (오른쪽)로 처리 된 Satureja 잎의 세포 구조를 보여주고 초음파 처리에 의한 세포벽의 훨씬 더 효율적인 파괴를 보여줍니다. 그 결과, 초음파 수소 증류법은 약 40 %의 기존 수소 증류법과 비교하여 에센셜 오일 추출을 향상시킵니다.
Sonicators for the Production of Floral Extracts
Hielscher sonicators are renowned for their excellence in the production of flowers and blossoms, particularly due to their advanced homogenization and extraction capabilities. Offering sonicators at all sizes and processing capacities – from 50 watts up to 16,000 watts per sonicator – , Hielscher high-performance ultrasonicators facilitate the efficient and precise extraction of bioactive compounds, essential oils, and fragrances from botanical materials. By employing cutting-edge ultrasonic technology, Hielscher sonicators ensures optimal yield and quality of extracts, making them indispensable for producing high-value botanicals, fragrances, active ingredients, and nutritional supplements. Additionally, their robust design and user-friendly interface make them ideal for quality assessment, providing reliable and reproducible results in both research and industrial applications.
- 고능률
- 최첨단 기술
- 신뢰도 & 견고성
- 조정 가능하고 정밀한 공정 제어
- 일괄 & 인라인
- 모든 볼륨에 대해
- 인텔리전트 소프트웨어
- 스마트 기능(예: 프로그래밍 가능, 데이터 프로토콜링, 원격 제어)
- 쉽고 안전한 작동
- 낮은 유지 보수
- CIP(clean-in-place, 클린-인-플레이스)
설계, 제조 및 컨설팅 – 독일에서 만든 품질
Hielscher 초음파는 최고의 품질과 디자인 표준으로 잘 알려져 있습니다. 견고 함과 쉬운 작동으로 초음파를 산업 시설에 원활하게 통합 할 수 있습니다. 거친 조건과 까다로운 환경은 Hielscher 초음파기로 쉽게 처리 할 수 있습니다.
Hielscher 초음파는 ISO 인증 회사이며 최첨단 기술과 사용자 친화성을 갖춘 고성능 초음파에 특히 중점을 둡니다. 물론, Hielscher 초음파는 CE를 준수하며 UL, CSA 및 RoHs의 요구 사항을 충족합니다.
문의! / 저희에게 물어보세요!
알아 둘 만한 가치가 있는 사실
What is the difference between rose water, rose absolute and rose hydrolat?
Rose water, rose absolute, and rose hydrolat are all derived from roses, but they differ in their production methods and chemical composition.
Rose Water: Rose water is a byproduct of the steam distillation process used to extract rose essential oil. It contains the water-soluble components of the rose petals, imparting a delicate floral fragrance and flavor. Rose water is commonly used in culinary applications, skincare products, and aromatherapy due to its pleasant scent and potential health benefits.
To read more about ultrasonically-intensified hydrodistillation, click here!
Rose Absolute: Rose absolute is an aromatic oil extracted from rose petals through solvent extraction, usually with hexane or another solvent. This method is more efficient for extracting the fragrant compounds from the petals, resulting in a highly concentrated and fragrant oil.
Rose absolute is commonly used in perfumery and high-end cosmetic products for its intense and long-lasting floral scent.
Rose Hydrolat (Rose Floral Water):
Rose hydrolat, also known as rose floral water, rose hydrosol or rose distillate, is the water remaining after steam distillation of rose petals to extract rose essential oil. It contains water-soluble plant compounds as well as some of the essential oil molecules, giving it a mild fragrance and therapeutic properties. Rose hydrolat offers a similar aroma profile but has a more diluted water-based formulation.
Rose hydrolat is often used in skincare as a toner, facial mist, or ingredient in cosmetic formulations due to its hydrating, soothing, and refreshing qualities.
Each of these rose-derived liquids can be manufactures by ultrasonic extraction and has unique characteristics and benefits, which make them a precious ingredient in culinary, cosmetic, and therapeutics.
Bioactive Compounds in Botanical Extracts
Bioactive compounds are naturally occurring chemical substances found in plants, animals, and other organisms that have effects on living tissues. They play significant roles in health promotion and disease prevention. These compounds can be broadly classified into several categories based on their chemical structures and biological functions. Here are the main types of bioactive compounds:
- 폴리페놀
Polyphenols are a diverse group of compounds known for their antioxidant properties. They can be further subdivided into several classes:
Flavonoids : Includes flavonols (e.g., quercetin), flavones (e.g., luteolin), flavanones (e.g., hesperidin), flavanols (e.g., catechins), isoflavones (e.g., genistein), and anthocyanins (e.g., cyanidin).
Phenolic acids : Includes hydroxybenzoic acids (e.g., gallic acid) and hydroxycinnamic acids (e.g., caffeic acid).
Tannins : Condensed tannins (proanthocyanidins) and hydrolysable tannins (gallotannins).
Stilbenes : Resveratrol is the most well-known stilbene.
Lignans : Found in seeds, particularly flaxseeds (e.g., secoisolariciresinol). - 테르페노이드
Terpenoids, also known as isoprenoids, are derived from five-carbon isoprene units and include:
Carotenoids : Includes carotenes (e.g., β-carotene) and xanthophylls (e.g., lutein, zeaxanthin).
Monoterpenes : Such as limonene and menthol.
Sesquiterpenes : Such as curcumin.
Diterpenes : Such as taxol.
Triterpenes : Such as saponins and sterols (e.g., β-sitosterol).
Tetraterpenes : Such as lycopene and astaxanthin. - Alkaloids
Alkaloids are nitrogen-containing compounds that often have pronounced physiological effects:
Pyrrolidine and piperidine alkaloids : Such as nicotine.
Tropane alkaloids : Such as atropine and cocaine.
Quinoline alkaloids : Such as quinine.
Isoquinoline alkaloids : Such as morphine and codeine.
Indole alkaloids : Such as strychnine and reserpine.
Imidazole alkaloids : Such as pilocarpine.
Pyridine alkaloids : Such as piperine. - 배당체
Glycosides consist of a sugar moiety bound to a non-sugar moiety (aglycone). They include:
Cardiac glycosides : Such as digoxin.
Anthraquinone glycosides : Such as aloin.
Flavonoid glycosides : Where the aglycone is a flavonoid (e.g., rutin). - 사포닌
Saponins are glycosides with soap-like properties:
Steroidal saponins : Found in plants like yams (e.g., diosgenin).
Triterpenoid saponins : Found in legumes (e.g., soyasaponins). - Organosulfur Compounds
These compounds contain sulfur and have notable health benefits:
Glucosinolates : Found in cruciferous vegetables (e.g., sulforaphane).
Thiosulfinates : Such as allicin from garlic. - 비타민
Vitamins are organic compounds required in small quantities for health:
Fat-soluble vitamins : Includes vitamins A (retinoids), D (calciferols), E (tocopherols and tocotrienols), and K (phylloquinone and menaquinones).
Water-soluble vitamins : Includes B-complex vitamins (e.g., B1, B2, B3, B5, B6, B7, B9, B12) and vitamin C (ascorbic acid). - 지방산
Fatty acids, especially essential fatty acids, play critical roles in health:
Omega-3 fatty acids : Such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA).
Omega-6 fatty acids : Such as linoleic acid (LA) and arachidonic acid (AA). - Peptides and Proteins
Bioactive peptides and proteins have various physiological effects:
Antimicrobial peptides : Such as defensins.
Enzyme inhibitors : Such as ACE inhibitors from milk proteins. - 다당류
Polysaccharides have immunomodulatory and other health benefits:
β-Glucans : Found in oats and mushrooms.
Glycosaminoglycans : Such as hyaluronic acid and chondroitin sulfate.
Each of these bioactive compounds offers a wide array of benefits from antioxidant activity to anti-inflammatory effects. Sonication is a highly efficient technique to isolate these bioactive compounds from plants.
문헌 / 참고문헌
- Turrini, F.; Beruto, M.; Mela, L.; Curir, P.; Triglia, G.; Boggia, R.; Zunin, P.; Monroy, F. (2021): Ultrasound-Assisted Extraction of Lavender (Lavandula angustifolia Miller, Cultivar Rosa) Solid By-Products Remaining after the Distillation of the Essential Oil. Applied Sciences 11, 2021.
- Turrini, Federica; Donno, Dario; Beccaro, Gabriele; Zunin, Paola; Pittaluga, Anna; Boggia, Raffaella (2019): Pulsed Ultrasound-Assisted Extraction as an Alternative Method to Conventional Maceration for the Extraction of the Polyphenolic Fraction of Ribes nigrum Buds: A New Category of Food Supplements Proposed by The FINNOVER Project. Foods. 8. 466; 2019
- Sitthiya, K.; Devkota, L.; Sadiq, M.B.; Anal A.K. (2018): Extraction and characterization of proteins from banana (Musa Sapientum L) flower and evaluation of antimicrobial activities. Journal of Food Science and Technology (February 2018) 55(2):658–666.
- Sánchez-Hernández E., Balduque-Gil J., González-García V., Barriuso-Vargas J.J., Casanova-Gascón J., Martín-Gil J., Martín-Ramos P. (2023): Phytochemical Profiling of Sambucus nigra L. Flower and Leaf Extracts and Their Antimicrobial Potential against Almond Tree Pathogens. International Journal of Molecular Sciences, 2023.
- Elez Garofulić, I., Zorić, Z., Pedisić, S., Brnčić, M. and Dragović-Uzelac, V. (2018): UPLC-MS2 Profiling of Blackthorn Flower Polyphenols Isolated by Ultrasound-Assisted Extraction. Journal of Food Science, 83, 2018. 2782-2789.