Ultraäänilaboratoriolaitteet
Hielscher lab sonicators are compact, easy-to-use and extremely versatile. They can be used for the processing of a variety of organic and inorganic materials in a wide range of volumes. Powerful ultrasonic homogenizers are available for intense direct and indirect sonication. Hielscher offers the ideal sonicator for your sample preparation task.
Typical applications of ultrasonic homogenizers include sample preparation, disintegrating and cell lysis, homogenizing, dispersing and disaggregation, particle size reduction and the acceleration of chemical reactions (sonochemistry). Hielscher offers a wide range of ultrasonic homogenizers to be used for these applications in a wide range of volumes from approx. 50µL to 4000mL. The selection of the ultrasonic processor depends on the sample volume to be sonicated.
Alla oleva taulukko antaa sinulle viitteitä laboratoriokokoisten ultraäänilaitteiden likimääräisestä käsittelykapasiteetista:
Suositellut laitteet | Erän tilavuus | Virtausnopeus |
---|---|---|
UIP400MTP 96-kuoppainen levysonikaattori | monikuoppa- / mikrotiitterilevyt | n.a. |
VialTweeter | vials and tubes with 0.5 to 1.5mL | n.a. |
UP100H Probe-type Sonicator | 1 - 500 ml | 10 - 200 ml / min |
UP200Ht, UP200St Koetintyyppiset sonikaattorit | 10 - 1000ml | 20–200 ml/min |
UP400St Probe-type Sonicator | 10 - 2000ml | 20–400 ml/min |
Ultraääni CupHorn | CupHorn injektiopulloille tai dekantterilasille | n.a. |
GDmini2 | ultraääni mikrovirtausreaktori | n.a. |
Ultraääniseulan ravistin | Ultrasonic lab sieving system | n.a. |
Important Features of a Quality Sonicator
If you want to buy a lab homogenizer, you might wonder what quality criterions and features you should look for. Hielscher probe-type sonicators are state-of-the-art ultrasonic homogenizers designed to meet your diverse research, analytical and industrial needs with precision, efficiency and user-comfort. Featuring a wide range of exceptional features and quality traits, Hielscher sonicators empower you to achieve consistent and reliable results across manifold applications. Below, you get an overview of the most important quality features of Hielscher ultrasonic homogenizers.
- Power and Intensity: All Hielscher sonicators boasts ample power capacity, which ensures rapid and effective sample preparation such as tissue homogenization, cell disruption, protein isolation and particle dispersion. Choose the ideal sonicator for your application and sample volume: Hielscher lab homogenizers are available with 50, 100, 200, and 400 watts ultrasound power.
- Amplitude Control: Achieve fine-tuned and highly efficient homogenization, even for delicate samples, thanks to the precise amplitude control of all Hielscher sonicators. Dial the an accurate amplitude for your required process intensity. The automatic frequency tuning makes sure your sonicator performs at the optimal operational frequency.
- Sonotrode Sizes and Shapes: Choose from a selection of sonotrode/probe geometries and sizes to suit your sample container and achieve optimal homogenization efficiency. Made from robust and corrosion-resistant materials such as titanium, ceramic or glass, our ultrasonic probes ensure high-performance and compatibility with a wide range of sample types.
- Digital Control: All sonicators from 200 watts up offer state-of-the-art digital control via coloured touch-display, an easy-to-navigate menu, pre-programmable settings and browser remote control. As a unique additional feature, customers love the sample illumination of Hielscher digital probe-type lab sonicators.
- Safety Features: Prioritizing your safety, Hielscher ultrasonic homogenizers include features such as dry-running and programmable auto-stop functions. Designed with a clean interface for intuitive operation, Hielscher sonicators are user-friendly as well as easy- and safe-to-operate.
- Sample Volume and Vessel Compatibility: Whether you are working in a laboratory or an industrial setting, Hielscher sonicators are available at different sizes, power capacities and with accessories to accommodate a broad spectrum of applications, sample types and volumes.
- Ease of Cleaning and Maintenance: Disassembly and cleaning are a breeze, preventing sample contamination between samples and ensuring equipment longevity. CIP (clean-in-place) and SIP (sterilize-in-place) facilitate proper and quick cleaning. Sonotrodes/probes and flow cells are autoclavable.
Ultrasonic Flow Cell Reactors for Larger Volume Processing
Hielscher ultrasonic laboratory devices can run continuously in 24/7 operation without overheating. Thereby, Hielscher sonicators can be reliably used for the continuous sonication using a flow cell in inline processing. By using the laboratory devices in combination with flow cell reactors, you can comfortably process larger sample volumes. In this case, the liquid is pumped into the reactor made of glass or stainless steel. For example, a UP400St can process approx. 10 to 50 litres per hour. In the flow cell, the sample it is exposed to defined intense sonication before it reaches the exit of the reactor cell. In order to cool heat-sensitive material during sonication, the flow cells are equipped with a cooling jacket to increase heat dissipation.
Ota yhteyttä! / Kysy meiltä!
Homogenizers Designed for the Lab
Hielscher ultrasonic laboratory devices are designed for the lab. They are very simple to setup. The handheld and stand-mounted devices combine ultrasonic generator (power supply) and transducer (converter) in a single housing. This saves desktop space and weight. Hielscher is the only manufacturer of handheld ultrasonic processors. The only plug to be connected is the main power plug, that fits into standard 115V~ or 230V~ outlets. The operation of the ultrasonic homogenizers is very simple. The devices are tuned to the optimal resonant frequency automatically, so there is no need for manual adjustments. The ultrasonic amplitude is adjustable from 20 through 100%. Alternatively to the continuous operation a cycle of intense sonication bursts can be a adjusted, e.g. for the sonication of heat-sensitive tissue.
Accuracy for Reproducible Results
For the preparation of samples as well as for the development of new applications, it is important to have a good control over all sonication parameters in order to obtain reproducible results. Hielscher ultrasonic devices allow you to control the amplitude of sonication. This is the most important sonication parameter. The mechanical amplitude is monitored constantly in the device to eliminate any deviation from the adjusted value. This makes the amplitude independent of other factors, such as immersion depth, liquid temperature or viscosity. Therefore, you can repeat each sonication by selecting the same amplitude again.
Sound Protection Boxes for Noise Reduction
When applied to liquids, ultrasound causes cavitation. The cavitational noise is in the human audible range. Sound protection boxes are available for the ultrasonic laboratory devices to reduce the noise emission to more convenient levels. This is recommended for typical laboratory environments. The sound protection boxes include a stand pole and an adjustable table (acrylic glass) to hold the sample. Click here to read more about ultrasonic noise emission!
Kirjallisuus / Viitteet
Find selected studies in which Hielscher sonicators are use for various sample preparation tasks such as cell disruption, DNA fragmentation, protein isolation and fractionation as well as dispersing and emulsification.
- Nico Böhmer, Andreas Dautel, Thomas Eisele, Lutz Fischer (2012): Recombinant expression, purification and characterisation of the native glutamate racemase from Lactobacillus plantarum NC8. Protein Expr Purif. 2013 Mar;88(1):54-60.
- Brandy Verhalen, Stefan Ernst, Michael Börsch, Stephan Wilkens (2012): Dynamic Ligand-induced Conformational Rearrangements in P-glycoprotein as Probed by Fluorescence Resonance Energy Transfer Spectroscopy. J Biol Chem. 2012 Jan 6;287(2): 1112-27.
- Claudia Lindemann, Nataliya Lupilova, Alexandra Müller, Bettina Warscheid, Helmut E. Meyer, Katja Kuhlmann, Martin Eisenacher, Lars I. Leichert (2013): Redox Proteomics Uncovers Peroxynitrite-Sensitive Proteins that Help Escherichia coli to Overcome Nitrosative Stress. J Biol Chem. 2013 Jul 5; 288(27): 19698–19714.
- Elahe Motevaseli, Mahdieh Shirzad, Seyed Mohammad Akrami, Azam-Sadat Mousavi, Akbar Mirsalehian, Mohammad Hossein Modarressi (2013): Normal and tumour cervical cells respond differently to vaginal lactobacilli, independent of pH and lactate. ed Microbiol. 2013 Jul; 62(Pt 7):1065-1072.
- Joanna Kopecka, Giuseppina Salzano, Ivana Campia, Sara Lusa, Dario Ghigo, Giuseppe De Rosa, Chiara Riganti (2013): Insights in the chemical components of liposomes responsible for P-glycoprotein inhibition. Nanomedicine: Nanotechnology, Biology, and Medicine 2013.
- Fernandes, Luz; Santos, Hugo; Nunes-Miranda, J.; Lodeiro, Carlos; Capelo, Jose (2011): Ultrasonic Enhanced Applications in Proteomics Workflows: single probe versus multiprobe. Journal of Integrated OMICS 1, 2011.
- Priego-Capote, Feliciano; Castro, María (2004): Analytical uses of ultrasound – I. Sample preparation. TrAC Trends in Analytical Chemistry 23, 2004. 644-653.
- Welna, Maja; Szymczycha-Madeja, Anna; Pohl, Pawel (2011): Quality of the Trace Element Analysis: Sample Preparation Steps. In: Wide Spectra of Quality Control; InTechOpen 2011.