• Ultrasonic seed priming is a useful pre-sowing technique to replenish seeds with water and nutrients and to stimulate germination.
• 초음파 처리는 종자 휴면을 깨고 발아 특성을 개선하기위한 효율적인 기술입니다.
• Hielscher 초음파 장비는 종자 발아 및 프라이밍의 신뢰할 수있는 결과를 위해 정밀하게 제어 할 수 있습니다.

Ultrasonic Seed Priming, Germination and Sprouting

Ultrasonic seed priming refers to a process of treating seeds with ultrasound waves at approx. 20 kHz in water or other suitable nutrient solutions to enhance their germination and early growth. A rapid germination and seedling emergence are critical factors for a successful establishment of plants and crops. Dormancy breaking and germination stimulation are important for proliferation and early production of plant seedlings. Ultrasonication of seeds in water, nutrient solution or an osmoticum improves the germination rate and speed and show a higher germination percentage than untreated seeds.

초음파 처리 된 렌즈 콩 (40Ws / g w / UP200St)에 비해 비 초음파 렌즈콩 : 초음파 렌즈콩은 더 높은 gemination 속도와 긴 촬영을 보여줍니다.

Advantages of Ultrasonic Seed Priming

Intense ultrasound of approx. 20kHz show significant impact on the seed coat as well as on the mass transfer and intracellular processes. Below, you can find details about the major effects of sonication on seeds and their related benefits.

• Increased germination rate: Ultrasonic seed priming can improve seed germination rates by breaking down seed dormancy, increasing water uptake, and activating enzymes that promote seed growth. Since sonication can activate enzymes within the seed that are responsible for breaking down stored nutrients and initiating germination.
• Increasing water uptake: Sonication can perforate the seed coat and cell walls, so that the seed can subsequently increase its permeability to water and improve water and nutrient uptake. This gives primed seeds an advantage especially in dry or nutrient-depleted soil.
• Accelerated seedling emergence: Ultrasound treatment for seed priming can reduce the time it takes for seedlings to emerge from the soil, which can be particularly beneficial in situations where a quick establishment of crops is desired. By breaking down the seed coat and improve water permeability, promoting the swelling and germination of seeds, ultrasound can interrupt seed dormancy.
• Enhanced seedling growth: Ultrasonic seed priming can stimulate the growth of seedlings by promoting the development of roots, stems, and leaves. This can result in stronger, healthier plants with better yields.
• Improved seedling tolerance to stress: Ultrasonic seed priming increases the ability of seedlings to withstand environmental stressors such as drought, salinity, and high temperatures.
• Reduced seedling mortality: Ultrasonic seed priming can reduce seedling mortality rates by promoting uniform germination and early growth, which can help to prevent the loss of seedlings due to uneven emergence or poor growth.

Due to these positive effects, ultrasonically induced seed priming and germination improves the quality and quantity of crops leading to more successful germination, reducing production costs, and increasing sustainability in agriculture.

애기장대 씨의 주사 전자 현미경 검사법 (SEM) 사진.
A-c 종자 30초 동안 24°C에서 초음파 처리를 실시하였다.
d, e 드라이 시드, f, g 씨앗은 4 °C에서 4 일 동안 물에 배치,
h, i, germinated seeds.
Images and Study: ©Lopez-Ribera et al. 2017

초음파 프라이밍

만니톨, 폴리에틸렌 글리콜(오스모프림), 식염수 용액(예: CaCl)과 같은 소모티카에서 씨앗 프라이밍₂, NaCl 또는 카소₄) (halopriming) and in water (hydropriming) can be easily improved by the application of ultrasound waves. Ultrasonically induced shell fragmentation, perforation and enlargement of the pore size of seeds lead to a higher water and nutrient retention capacity in seeds and grains resulting in improved hydration. Sonication improves the mass transfer between the seed and the surrounding solution resulting in an increased uptake of solution (i.e. water, nutrients etc.) into the seed is achieved. Ultrasonically assisted priming is an economical, simple and a safe technique for increasing the capacity of seeds to osmotic adjustment and enhancing seed germination, seedling establishment and crop production under stressed conditions.
Furthermore, ultrasonication causes endosperm modification, such as starch degradation by sonication, which increases the rate of enzyme-catalyzed hydrolysis reactions within the seeds. An increased enzyme activity and hydrolysis accelerates germination and embryo growth as result of the ultrasonic treatment.
초음파 프라이밍에 대한 자세한 내용은 여기를 참조하십시오!

Ultrasonicators for Seed Priming and Germination

Hielscher Ultrasonics supplies ultrasonic equipment for improved seed priming including hydro-priming, osmo-priming, and halo-priming. Compact ultrasonicators for R+D and lab work allow for testing and establishing the optimum ultrasonic process parameters, whilst industrial-grade ultrasonic equipment are implemented in the large-scale treatment of commercially traded seeds in a convenient and highly efficient flow-through ultrasonic priming treatment.
In order to improve germination of seed by sonication, precisely controllable and adjustable ultrasonic systems are required. Since ultrasound waves are used to stimulate physiological activities of vegetal cells, the intensity of sonication must be adapted to the specific type of seed and cultivar. Hielscher ultrasonic systems can be set to mild amplitudes delivering the cavitation and/or oscillation required to prime seeds for optimal germination and vigour. Seed treatment can be applied in batch as well as in continuous flow mode. The broad spectrum of accessories allow for the easy integration of ultrasound into existing premises.
Hielscher ultrasonic systems increase the potential for high seedling performance after sowing.

문학 / 참고 문헌

• 얄다가드 엠; 레자 모르타자비 S.A.; 타바타베이 F. (2008) : 보리 종자의 발아를 가속화하고 향상시키기위한 프라이밍 기술로 초음파의 응용 프로그램 : 타구치 접근법에 의한 방법의 최적화. 양조 연구소의 저널 114, 발행1, 2008. 14-21.
• 마치코와 T.; 쿨라타나락 T.; Wonprasaid S. (2013) : 합성 해바라기 씨앗의 발아에 초음파 치료의 효과. 과학의 세계 아카데미, 공학 및 기술 농업 및 생물 시스템 공학의 국제 저널 Vol:7, No:1, 2013.
• 나자리 엠; Eteghadipour M. (2017) : 씨앗에 초음파의 영향 : 미니 리뷰. 아그리 레스 & 기술 볼륨 6 문제 3 – 2017년 4월.