Hielscher ultratovush to'qimalarining gomogenizatorlaridan qanday foydalanish kerak
Before purifying or characterizing intracellular macromolecules such as proteins, organelles, enzymes, or active compounds, it is essential to employ an efficient method for tissue lysis and cell disintegration. Ultrasonication is a highly effective technique for cell preparation, quickly releasing intracellular materials into a buffer solution. The mechanical shear forces generated during ultrasonic tissue homogenization can be precisely adjusted to suit specific materials. This allows for gentle preparation of soft tissues or DNA/RNA shearing with milder sonication, as well as intense ultrasonic cavitation for destructive cell disruption (e.g., for nannochloropsis, yeast).
Below is a selection of tissues, cells, and other biological matter with recommended sonication protocols and guidelines for efficiently preparing your samples using an ultrasonic tissue homogenizer or cell crusher.
Lab ultra-sonicator UP200Ht (200W, 26kHz) for sample preparation tasks
How to Prepare your Lysates, Homogenates and Extracts from Biological Materials
Alifbo tartibida:
Acetobacter suboxydans
Actinomyces
ALP and LDH activity
Ultrasonik dastur:
Determination of ALP and LDH activity and protein content
Frozen cell samples were thawed for 20 min on ice, followed by cell lysis with PBS containing 1% Triton X-100 for 50 min on ice. During cell lysis each sample was sonicated for 1 min at 80 W with an ultrasonic processor UP100H (Hielscher Ultrasonics).
Qurilma tavsiyasi:
UP100H
Ma'lumotnoma/tadqiqot maqolasi:
Bernhardt, A. et al. (2008): Mineralised collagen—an artificial, extracellular bone matrix—improves osteogenic differentiation of bone marrow stromal cells.
Determination of ALP and LDH activity and protein content
Frozen cell samples were thawed for 20 min on ice, followed by cell lysis with PBS containing 1% Triton X-100 for 50 min on ice. During cell lysis each sample was sonicated for 1 min at 80 W with an ultrasonic processor UP100H (Hielscher Ultrasonics).
Qurilma tavsiyasi:
UP100H
Ma'lumotnoma/tadqiqot maqolasi:
Bernhardt, A. et al. (2008): Mineralised collagen—an artificial, extracellular bone matrix—improves osteogenic differentiation of bone marrow stromal cells.
Amorphous tricalcium phosphate (ATCP)
Ultrasonik dastur:
ATCP nano-particles, which are an exceptionally reactive precursor for the formation of hydroxyapatite, were dispersed in chloroform containing 5 % (w/w) Tween20 referred to PLGA using the Hielscher ultrasonic device UP400S at 320W for 5 min. applying pulsed intervals (50%) to allow for relaxation of the particles.
Qurilma tavsiyasi:
UP400S
Ma'lumotnoma/tadqiqot maqolasi:
Mohn, Dirk; Ege, Duygu; Feldman, Kirifl; Schneider, Oliver D.; Imfeld, Thomas; Boccaccini, Aldo R. (2014): Spherical calcium phosphate nanoparticle fillers allow polymer processing of bone fixation devices with high bioactivity. The Free Library 01 May 2010. 21 January 2014.
ATCP nano-particles, which are an exceptionally reactive precursor for the formation of hydroxyapatite, were dispersed in chloroform containing 5 % (w/w) Tween20 referred to PLGA using the Hielscher ultrasonic device UP400S at 320W for 5 min. applying pulsed intervals (50%) to allow for relaxation of the particles.
Qurilma tavsiyasi:
UP400S
Ma'lumotnoma/tadqiqot maqolasi:
Mohn, Dirk; Ege, Duygu; Feldman, Kirifl; Schneider, Oliver D.; Imfeld, Thomas; Boccaccini, Aldo R. (2014): Spherical calcium phosphate nanoparticle fillers allow polymer processing of bone fixation devices with high bioactivity. The Free Library 01 May 2010. 21 January 2014.
antosiyaninlar
Ultrasonik dastur:
Anthocyanins: di-glucosides, mono-glucoside, acylated monoglucosides and acylated di-glucosides of peonidin, malvidin, cyanidin, petunidin and delphinidin:Extraction from grape skin in 40 sec.; pH 5.0; ratio of material/extraction solvent of 1:6.
Qurilma tavsiyasi:
UP100H
Anthocyanins: di-glucosides, mono-glucoside, acylated monoglucosides and acylated di-glucosides of peonidin, malvidin, cyanidin, petunidin and delphinidin:Extraction from grape skin in 40 sec.; pH 5.0; ratio of material/extraction solvent of 1:6.
Qurilma tavsiyasi:
UP100H
Anthraquinones
Ultrasonik dastur:
Extraction of Anthraquinones from the roots of Morinda citrifolia
Qurilma tavsiyasi:
UP400S
Extraction of Anthraquinones from the roots of Morinda citrifolia
Qurilma tavsiyasi:
UP400S
Apricot seeds
Ultrasonik dastur:
Ultrasonic pre-treatment before extracting oil from the seeds of Jatropha curcas L. to improve extraction.
Qurilma tavsiyasi:
UP400S
Ultrasonic pre-treatment before extracting oil from the seeds of Jatropha curcas L. to improve extraction.
Qurilma tavsiyasi:
UP400S
Artemisia selengensis Turcz
Ultrasonik dastur:
Extraction of Rutin (1.0g milled sample in 30 mL methanol) at 25°C in 5-10 min.
Qurilma tavsiyasi:
UP50H
Extraction of Rutin (1.0g milled sample in 30 mL methanol) at 25°C in 5-10 min.
Qurilma tavsiyasi:
UP50H
Aspergillus flavus
Ultrasonik dastur:
Ultrasonic inactivation of Aspergillus flavus in Sabouraud growth medium
Qurilma tavsiyasi:
UP200S
Ultrasonic inactivation of Aspergillus flavus in Sabouraud growth medium
Qurilma tavsiyasi:
UP200S
B. sphaericus / Bacillus sphaericus
Bacillus anthracis sterne 34F2 spores
Ultrasonik dastur:
Ultrasonic removal of Bacillus anthracis sterne 34F2, Bacillus cereus ATCC 21281, and Bacillus thuringiensis ATCC 33680 spores. The use of 150 ppm of sodium hypochlorite along with ultrasound did lead to spore inactivation.
Qurilma tavsiyasi:
UP200S at 100% amplitude
Ma'lumotnoma/tadqiqot maqolasi:
Pamarthi, S.R. et al.: Effectiveness of Ultrasound in Desoiling Bacillus spp spores Embedded in Complex Food Matrices Attached to Various Contact Surfaces.
Ultrasonic removal of Bacillus anthracis sterne 34F2, Bacillus cereus ATCC 21281, and Bacillus thuringiensis ATCC 33680 spores. The use of 150 ppm of sodium hypochlorite along with ultrasound did lead to spore inactivation.
Qurilma tavsiyasi:
UP200S at 100% amplitude
Ma'lumotnoma/tadqiqot maqolasi:
Pamarthi, S.R. et al.: Effectiveness of Ultrasound in Desoiling Bacillus spp spores Embedded in Complex Food Matrices Attached to Various Contact Surfaces.
Bacillus cereus ATCC 21281 spores
Ultrasonik dastur:
Ultrasonic removal of Bacillus anthracis sterne 34F2, Bacillus cereus ATCC 21281, and Bacillus thuringiensis ATCC 33680 spores. The use of 150 ppm of sodium hypochlorite along with ultrasound did lead to spore inactivation.
Qurilma tavsiyasi:
UP200S at 100% amplitude
Ma'lumotnoma/tadqiqot maqolasi:
Pamarthi, S.R. et al.: Effectiveness of Ultrasound in Desoiling Bacillus spp spores Embedded in Complex Food Matrices Attached to Various Contact Surfaces.
Ultrasonic removal of Bacillus anthracis sterne 34F2, Bacillus cereus ATCC 21281, and Bacillus thuringiensis ATCC 33680 spores. The use of 150 ppm of sodium hypochlorite along with ultrasound did lead to spore inactivation.
Qurilma tavsiyasi:
UP200S at 100% amplitude
Ma'lumotnoma/tadqiqot maqolasi:
Pamarthi, S.R. et al.: Effectiveness of Ultrasound in Desoiling Bacillus spp spores Embedded in Complex Food Matrices Attached to Various Contact Surfaces.
Bacillus subtilis
Ultrasonik dastur:
High power, low frequency ultrasound in low volumes of bacterial suspension results in a continuous reduction in bacterial cell numbers i.e. the kill rate predominates. In larger volumes, sonication results in an initial rise in cell numbers suggesting declumping of the bacteria but this initial rise then falls as the declumping finishes and the kill rate becomes more important.
Qurilma tavsiyasi:
UP200St
Ma'lumotnoma/tadqiqot maqolasi:
Joyce, E.; Phull, S. S.; Lorimer, J. P.; Mason, T. J. (2003): The development and evaluation of ultrasound for the treatment of bacterial suspensions. A study of frequency, power and sonication time on cultured Bacillus species. Ultrason. Sonochem. 10/2003. pp. 315-318.
High power, low frequency ultrasound in low volumes of bacterial suspension results in a continuous reduction in bacterial cell numbers i.e. the kill rate predominates. In larger volumes, sonication results in an initial rise in cell numbers suggesting declumping of the bacteria but this initial rise then falls as the declumping finishes and the kill rate becomes more important.
Qurilma tavsiyasi:
UP200St
Ma'lumotnoma/tadqiqot maqolasi:
Joyce, E.; Phull, S. S.; Lorimer, J. P.; Mason, T. J. (2003): The development and evaluation of ultrasound for the treatment of bacterial suspensions. A study of frequency, power and sonication time on cultured Bacillus species. Ultrason. Sonochem. 10/2003. pp. 315-318.
Barley's Alpha-amylase
Ultrasonik dastur:
Stimulating or inhibiting the activity of Barley’s Alpha-amylase: The sample (10 g barley seeds) was dispersed in 80 ml of tap water at direct sonication at ultrasonic intensity of 20, 60 and 100% amplitude, cyle of 50% and with additional agitation. The sonotrode was immersed about 9 mm into the solution. The solution was processed at constant temperature of 30C° for 5, 10 and 15 min.
Qurilma tavsiyasi:
UP200S, amplitudes: 20, 60 and 100%; cyle of 50%; Sonotrode S3, 30C°.
Ma'lumotnoma/tadqiqot maqolasi:
Yaldagard et al. (2008): Effect of Ultrasonic Power on the Activity of Barley’s Alpha-amylase from Post-sowing Treat of Seeds
Stimulating or inhibiting the activity of Barley’s Alpha-amylase: The sample (10 g barley seeds) was dispersed in 80 ml of tap water at direct sonication at ultrasonic intensity of 20, 60 and 100% amplitude, cyle of 50% and with additional agitation. The sonotrode was immersed about 9 mm into the solution. The solution was processed at constant temperature of 30C° for 5, 10 and 15 min.
Qurilma tavsiyasi:
UP200S, amplitudes: 20, 60 and 100%; cyle of 50%; Sonotrode S3, 30C°.
Ma'lumotnoma/tadqiqot maqolasi:
Yaldagard et al. (2008): Effect of Ultrasonic Power on the Activity of Barley’s Alpha-amylase from Post-sowing Treat of Seeds
Barnacle nauplii
Ultrasonik dastur:
Cell disruption/ killing of mesozooplankton: by sonication under following conditions of four flow rates (200, 400, 520 and 800 lh-1) and four amplitudes (25, 50, 75 and 100 %) a killing rate between 61 and 97 % has been achieved.
Qurilma tavsiyasi:
UIP2000hd
Ma'lumotnoma/tadqiqot maqolasi:
Viitaslo et al. (2005): Ozone, Ultraviolet Light, Ultrasound and Hydrogen Peroxide As Ballast Water Treatments – Experiments with Mesozooplankton In Low Saline- Brackish Water.
Cell disruption/ killing of mesozooplankton: by sonication under following conditions of four flow rates (200, 400, 520 and 800 lh-1) and four amplitudes (25, 50, 75 and 100 %) a killing rate between 61 and 97 % has been achieved.
Qurilma tavsiyasi:
UIP2000hd
Ma'lumotnoma/tadqiqot maqolasi:
Viitaslo et al. (2005): Ozone, Ultraviolet Light, Ultrasound and Hydrogen Peroxide As Ballast Water Treatments – Experiments with Mesozooplankton In Low Saline- Brackish Water.
Bifidobacteria strains: B. breve ATCC 15700, B. animalis subsp. Lactis (BB-12), B. longum (BB-46)
Ultrasonik dastur:
Bacteria cell destruction and release of ß-galactosidase from Bifidobacteria strains: B. breve ATCC 15700, B. animalis subsp. Lactis (BB-12), B. longum (BB-46): 100 mL pasteurized milk blended with bacteria culture was applied with ultrasound. Cavitation causes the destruction of the bacterial cells and at the same time, release of ß-galactosidase.
Qurilma tavsiyasi:
UIP1000hd: amplitude: 10%, power: 80W, amplitude: 100%, power: 200W; Sonotrode BS2d34; 15-30 min.
Ma'lumotnoma/tadqiqot maqolasi:
Hung et al. (2009): Ultrasound Aided Yogurt Fermentation with Probiotics.
Bacteria cell destruction and release of ß-galactosidase from Bifidobacteria strains: B. breve ATCC 15700, B. animalis subsp. Lactis (BB-12), B. longum (BB-46): 100 mL pasteurized milk blended with bacteria culture was applied with ultrasound. Cavitation causes the destruction of the bacterial cells and at the same time, release of ß-galactosidase.
Qurilma tavsiyasi:
UIP1000hd: amplitude: 10%, power: 80W, amplitude: 100%, power: 200W; Sonotrode BS2d34; 15-30 min.
Ma'lumotnoma/tadqiqot maqolasi:
Hung et al. (2009): Ultrasound Aided Yogurt Fermentation with Probiotics.
BL21(DE3) pAtHNL cells (Arabidopsis thaliana cells)
Ultrasonik dastur:
Cell disruption: 15 g BL21-(DE3)_pAtHNL cells were slowly suspended in 50 mM potassium phosphate buffer (pH 7.5) at 0 degC.
Qurilma tavsiyasi:
UP200S + sonotrode S14D: at 70W/cm2 (4 x 5 min), cooled in an ice bath
Ma'lumotnoma/tadqiqot maqolasi:
Okrob, D. et al (2009): Hydroxynitrile Lyase from Arabidopsis thaliana: Identification of Reaction Parameters for Enantiopure Cyanohydrin Synthesis by Pure and Immobilized Catalyst. Adv. Synth. Catal. 2011, 353, 2399 – 2408.
Cell disruption: 15 g BL21-(DE3)_pAtHNL cells were slowly suspended in 50 mM potassium phosphate buffer (pH 7.5) at 0 degC.
Qurilma tavsiyasi:
UP200S + sonotrode S14D: at 70W/cm2 (4 x 5 min), cooled in an ice bath
Ma'lumotnoma/tadqiqot maqolasi:
Okrob, D. et al (2009): Hydroxynitrile Lyase from Arabidopsis thaliana: Identification of Reaction Parameters for Enantiopure Cyanohydrin Synthesis by Pure and Immobilized Catalyst. Adv. Synth. Catal. 2011, 353, 2399 – 2408.
Blood cells (red and white)
Boldine from Boldo leaves (Peumus boldus Molina)
Ultrasonik dastur:
Boldodagi muhim faol birikma katexin ((2S,3R)-2-(3,4-dihidroksi-fenil)-3 dan tashqari boldin ((S)-2,9-digidroksi-1,10-dimetoksiaporfin) hisoblanadi. ,4-dihidro-1(2H)-benzopiran-3,5,7-triol) alkaloid va flavonoid fraktsiyasining asosiy komponentlari. boldo barglari. Boldin kuchli antioksidant bo'lib, peroksidlovchi erkin radikal vositachiligida zarar ko'radi va samarali gidroksil radikallarini tozalash vositasi sifatida ishlaydi.
Ekstraktsiya tartibi: Oddiy ekstraksiya jarayoni uchun ultrasonikator yordamida atmosfera bosimida 1 litr distillangan suv bilan boldo barglaridan namunalar olingan. UIP1000hd ommaviy va oqim rejimida. Ekstraksiya vaqti 10 dan 40 minutgacha, ultratovush intensivligi 10 dan 23 Vt/sm gacha.2, va harorat oralig'i 10 dan 70 ° C gacha. Eng yaxshi natijalarga quyidagi sharoitlarda erishiladi: ultratovush intensivligi 23 Vt/sm2 40 daqiqa davomida. 36 ° C haroratda
Natijalar: Tahlil natijalari shuni ko'rsatadiki, yuqori quvvatli sonikatsiya Boldoning o'simlik matritsasi materialining analit chiqarilishini an'anaviy usulga nisbatan ancha yaxshi stavkalarda kuchaytiradi: 30 daqiqada sonication orqali teng rentabellik chiqarildi. an'anaviy ekstraktsiya vaqti esa 2 soat edi.
Chemat (2013) va uning hamkasblari shuni ko'rsatdiki, ultratovush yordamida ekstraksiya o'simlik ekstraktsiyasining samaradorligini oshiradi, shu bilan birga ekstraktlarning yuqori konsentratsiyasida (bir xil miqdordagi erituvchi va o'simlik materiali) ekstraktsiya vaqtini qisqartiradi. Tahlil shuni ko'rsatdiki, optimallashtirilgan shartlar: sonikatsiya quvvati 23 Vt / sm2 bilan UIP1000hd 40 daqiqa davomida. va harorat 36 ° C. Ultratovushli ekstraksiyaning optimallashtirilgan parametrlari jarayon vaqti (120 min. oʻrniga 30 min.), yuqori rentabellik, yuqori energiya samaradorligi, yaxshilangan tozalik, yuqori xavfsizlik va mahsulot sifati boʻyicha anʼanaviy maseratsiyaga nisbatan yaxshiroq ekstraksiyani taʼminlaydi.
Qurilma tavsiyasi:
UIP1000hd sonotrode BS2d34 va oqim xujayrasi bilan
Ma'lumotnoma/tadqiqot maqolasi:
Petigny, L.; Périno-Issartier, S.; Wajsman, J.; Chemat, F. (2013): Boldo barglari (Peumus boldus Mol.) ning partiyaviy va doimiy ultratovush yordamida ekstraktsiyasi. Molekulyar fanlar xalqaro jurnali 14, 2013. 5750-5764.
Boldodagi muhim faol birikma katexin ((2S,3R)-2-(3,4-dihidroksi-fenil)-3 dan tashqari boldin ((S)-2,9-digidroksi-1,10-dimetoksiaporfin) hisoblanadi. ,4-dihidro-1(2H)-benzopiran-3,5,7-triol) alkaloid va flavonoid fraktsiyasining asosiy komponentlari. boldo barglari. Boldin kuchli antioksidant bo'lib, peroksidlovchi erkin radikal vositachiligida zarar ko'radi va samarali gidroksil radikallarini tozalash vositasi sifatida ishlaydi.
Ekstraktsiya tartibi: Oddiy ekstraksiya jarayoni uchun ultrasonikator yordamida atmosfera bosimida 1 litr distillangan suv bilan boldo barglaridan namunalar olingan. UIP1000hd ommaviy va oqim rejimida. Ekstraksiya vaqti 10 dan 40 minutgacha, ultratovush intensivligi 10 dan 23 Vt/sm gacha.2, va harorat oralig'i 10 dan 70 ° C gacha. Eng yaxshi natijalarga quyidagi sharoitlarda erishiladi: ultratovush intensivligi 23 Vt/sm2 40 daqiqa davomida. 36 ° C haroratda
Natijalar: Tahlil natijalari shuni ko'rsatadiki, yuqori quvvatli sonikatsiya Boldoning o'simlik matritsasi materialining analit chiqarilishini an'anaviy usulga nisbatan ancha yaxshi stavkalarda kuchaytiradi: 30 daqiqada sonication orqali teng rentabellik chiqarildi. an'anaviy ekstraktsiya vaqti esa 2 soat edi.
Chemat (2013) va uning hamkasblari shuni ko'rsatdiki, ultratovush yordamida ekstraksiya o'simlik ekstraktsiyasining samaradorligini oshiradi, shu bilan birga ekstraktlarning yuqori konsentratsiyasida (bir xil miqdordagi erituvchi va o'simlik materiali) ekstraktsiya vaqtini qisqartiradi. Tahlil shuni ko'rsatdiki, optimallashtirilgan shartlar: sonikatsiya quvvati 23 Vt / sm2 bilan UIP1000hd 40 daqiqa davomida. va harorat 36 ° C. Ultratovushli ekstraksiyaning optimallashtirilgan parametrlari jarayon vaqti (120 min. oʻrniga 30 min.), yuqori rentabellik, yuqori energiya samaradorligi, yaxshilangan tozalik, yuqori xavfsizlik va mahsulot sifati boʻyicha anʼanaviy maseratsiyaga nisbatan yaxshiroq ekstraksiyani taʼminlaydi.
Qurilma tavsiyasi:
UIP1000hd sonotrode BS2d34 va oqim xujayrasi bilan
Ma'lumotnoma/tadqiqot maqolasi:
Petigny, L.; Périno-Issartier, S.; Wajsman, J.; Chemat, F. (2013): Boldo barglari (Peumus boldus Mol.) ning partiyaviy va doimiy ultratovush yordamida ekstraktsiyasi. Molekulyar fanlar xalqaro jurnali 14, 2013. 5750-5764.
Bovine serum albumin (BSA)
Ultrasonik dastur:
Microencapsulation in poly(lactic-co-glycolic acid) in 40 sec.
Qurilma tavsiyasi:
GDmini
Ma'lumotnoma/tadqiqot maqolasi:
Freitas et al. (2005): Flow-through ultrasonic emulsification combined with static micromixing for aseptic production of microspheres by solvent extraction.
Microencapsulation in poly(lactic-co-glycolic acid) in 40 sec.
Qurilma tavsiyasi:
GDmini
Ma'lumotnoma/tadqiqot maqolasi:
Freitas et al. (2005): Flow-through ultrasonic emulsification combined with static micromixing for aseptic production of microspheres by solvent extraction.
Brain stem + adrenal gland
Ultrasonik dastur:
Dispersion and nucleotid analysis; sample size: 10 mg samples in 10 ml fluid.
Qurilma tavsiyasi:
UP50H
Dispersion and nucleotid analysis; sample size: 10 mg samples in 10 ml fluid.
Qurilma tavsiyasi:
UP50H
Candida albicans
Carbohydrates, polysaccharides and other functional compounds
Ultrasonik dastur:
Extraction of carbohydrates, polysaccharides and other functional compounds
Qurilma tavsiyasi:
UP200St
Extraction of carbohydrates, polysaccharides and other functional compounds
Qurilma tavsiyasi:
UP200St
Carnosic acid from rosemary
Ultrasonik dastur:
Extraction of carnosic acid, an active compound, from rosemary.
Qurilma tavsiyasi:
UP400S
Extraction of carnosic acid, an active compound, from rosemary.
Qurilma tavsiyasi:
UP400S
Kapsaitsinoidlar
Ultrasonik dastur:
Chili qalampiridan kapsaitsinoidlarni (kapsaitsin, nordigidrokapsaitsin) olish: kapsaitsinoidlar Capsicum frutescens qalampir quyidagi sharoitlarda ultratovushli ekstraktsiya yo'li bilan olingan: erituvchi: 95% (v/v) etanol, hal qiluvchi/massa nisbati 10 ml/g, 40 min. sonication ekstraktsiya vaqti, 45 ° C ekstraksiya harorati. Ekstrakt hosildorligi: kapsaitsinoidlarning 85%
Qurilma tavsiyasi:
UP400S
Chili qalampiridan kapsaitsinoidlarni (kapsaitsin, nordigidrokapsaitsin) olish: kapsaitsinoidlar Capsicum frutescens qalampir quyidagi sharoitlarda ultratovushli ekstraktsiya yo'li bilan olingan: erituvchi: 95% (v/v) etanol, hal qiluvchi/massa nisbati 10 ml/g, 40 min. sonication ekstraktsiya vaqti, 45 ° C ekstraksiya harorati. Ekstrakt hosildorligi: kapsaitsinoidlarning 85%
Qurilma tavsiyasi:
UP400S
Carotenoids, Beta-Carotenoids
cDNA
Ultrasonik dastur:
Poly-A RNA was purified with the Dynabeads mRNA purification kit (Invitrogen) following the manufacturer’s instructions and was treated for 30 min at 37°C with TURBO DNase (Ambion; 0.2 units/1 μg of RNA). First- and second-strand synthesis followed the manufacturer’s protocol. About 500ng of double-stranded cDNA was fragmented by sonication with Hielscher’s UTR200. DNA was parceled in 2% high-resolution agarose gel and 230–270 bp fragments were cut.
Qurilma tavsiyasi:
UTR200 yoki TD_CupHorn
Ma'lumotnoma/tadqiqot maqolasi:
Delft, J. van; Gaj, St.; Lienhard,M.; Albrecht, M. W.; Kirpiy, A.; Brauers, K.; Claessen, S.; Lizarraga, D.; Lehrach, H.; Herwig, R.; Kleinjans, J. (2012): RNA-Seq Provides New Insights in the Transcriptome Responses Induced by the Carcinogen Benzo[a]pyrene. Toxicological Sciences 130/2, 2012. 427–439.
Poly-A RNA was purified with the Dynabeads mRNA purification kit (Invitrogen) following the manufacturer’s instructions and was treated for 30 min at 37°C with TURBO DNase (Ambion; 0.2 units/1 μg of RNA). First- and second-strand synthesis followed the manufacturer’s protocol. About 500ng of double-stranded cDNA was fragmented by sonication with Hielscher’s UTR200. DNA was parceled in 2% high-resolution agarose gel and 230–270 bp fragments were cut.
Qurilma tavsiyasi:
UTR200 yoki TD_CupHorn
Ma'lumotnoma/tadqiqot maqolasi:
Delft, J. van; Gaj, St.; Lienhard,M.; Albrecht, M. W.; Kirpiy, A.; Brauers, K.; Claessen, S.; Lizarraga, D.; Lehrach, H.; Herwig, R.; Kleinjans, J. (2012): RNA-Seq Provides New Insights in the Transcriptome Responses Induced by the Carcinogen Benzo[a]pyrene. Toxicological Sciences 130/2, 2012. 427–439.
Caryophanon latum
Ultrasonik dastur:
Extraction of glucosamine, muramic acid, alanine, glutamic acid and lysine from caryophanon datum.
Qurilma tavsiyasi:
UP100H
Extraction of glucosamine, muramic acid, alanine, glutamic acid and lysine from caryophanon datum.
Qurilma tavsiyasi:
UP100H
tsellyuloza
Ultrasonik dastur:
Homogenization/ preparation of isotopically homogeneous cellulose.
Qurilma tavsiyasi:
UP200S; in an ice bath
Ma'lumotnoma/tadqiqot maqolasi:
Laumer et al. (2009): A novel approach for the homogenization of cellulose to use microamounts for stable isotope analyses.
Homogenization/ preparation of isotopically homogeneous cellulose.
Qurilma tavsiyasi:
UP200S; in an ice bath
Ma'lumotnoma/tadqiqot maqolasi:
Laumer et al. (2009): A novel approach for the homogenization of cellulose to use microamounts for stable isotope analyses.
Cellulose nanocrystals (CNC) prepared from eucalyptus cellulose CNCs
Ultrasonik dastur:
Cellulose nanocrystals (CNC) prepared from eucalyptus cellulose CNCs were modified by the reaction with methyl adipoyl chloride, CNCm, or with a mixture of acetic and sulfuric acid, CNCa. Therefore, freeze-dried CNCs, CNCm and CNCa were redispersed in pure solvents (EA, THF or DMF) at 0.1 wt%, bymagnetic stirring overnight at (24 ± 1) C, followed by 20 min in a sonication bath using UP100H Hielscher Ultrasonics (Germany), equipped with a 130 W/cm2 sonotrode, at 24 ± 1 degC. After that, CAB was added to the CNC dispersion, so that the final polymer concentration was 0.9 wt%.
Qurilma tavsiyasi:
UP100H; at 24 degC for 20 min.
Ma'lumotnoma/tadqiqot maqolasi:
Blachechen, L. S. et al (2013): Interplay of colloidal stability of cellulose nanocrystals and their dispersibility in cellulose acetate butyrate matrix. Cellulose 2013.
Cellulose nanocrystals (CNC) prepared from eucalyptus cellulose CNCs were modified by the reaction with methyl adipoyl chloride, CNCm, or with a mixture of acetic and sulfuric acid, CNCa. Therefore, freeze-dried CNCs, CNCm and CNCa were redispersed in pure solvents (EA, THF or DMF) at 0.1 wt%, bymagnetic stirring overnight at (24 ± 1) C, followed by 20 min in a sonication bath using UP100H Hielscher Ultrasonics (Germany), equipped with a 130 W/cm2 sonotrode, at 24 ± 1 degC. After that, CAB was added to the CNC dispersion, so that the final polymer concentration was 0.9 wt%.
Qurilma tavsiyasi:
UP100H; at 24 degC for 20 min.
Ma'lumotnoma/tadqiqot maqolasi:
Blachechen, L. S. et al (2013): Interplay of colloidal stability of cellulose nanocrystals and their dispersibility in cellulose acetate butyrate matrix. Cellulose 2013.
Cellulose from sugarcane bagasse
CHIP tahlili
Ultrasonik dastur:
Ultrasonication is used for cell lysis to release the chromatin. Mild (pulsed) sonication is used to fragment the chromatin. Furthermore, ultrasound accelerates the rate of antibody binding to target proteins and reduces thereby the immunoprecipitation time.
Qurilma tavsiyasi:
UP100H
Ma'lumotnoma/tadqiqot maqolasi:
Basselet, P. et al. (2008): Sample processing for DNA chip array-based analysis of enterohemorrhagic Escherichia coli (EHEC).
Lauri, A. (2005): Molecular analysis of petal development by X-ChIP and two-hybrid technology. Dissertation University of Cologne 2005.
Ultrasonication is used for cell lysis to release the chromatin. Mild (pulsed) sonication is used to fragment the chromatin. Furthermore, ultrasound accelerates the rate of antibody binding to target proteins and reduces thereby the immunoprecipitation time.
Qurilma tavsiyasi:
UP100H
Ma'lumotnoma/tadqiqot maqolasi:
Basselet, P. et al. (2008): Sample processing for DNA chip array-based analysis of enterohemorrhagic Escherichia coli (EHEC).
Lauri, A. (2005): Molecular analysis of petal development by X-ChIP and two-hybrid technology. Dissertation University of Cologne 2005.
xromatin
Ultrasonik dastur:
Shearing of chromatin
Qurilma tavsiyasi:
UP400S; at 30% amplitude and 0.5 cycle; on ice.
Ma'lumotnoma/tadqiqot maqolasi:
Oh et al. (2003): Acetyl-CoA Carboxylase Gene Is Regulated by Sterol Regulatory Element-binding Protein-1 in Liver.
Shearing of chromatin
Qurilma tavsiyasi:
UP400S; at 30% amplitude and 0.5 cycle; on ice.
Ma'lumotnoma/tadqiqot maqolasi:
Oh et al. (2003): Acetyl-CoA Carboxylase Gene Is Regulated by Sterol Regulatory Element-binding Protein-1 in Liver.
Chromatin extraction
Ultrasonik dastur:
The lysate of MEL DS19 cells was sonicated with 10 rounds of 20 s each at 70% of maximum output using the Hielscher 200W ultrasonic processor UP200H
Qurilma tavsiyasi:
UP200H; 70% of maximum output; pulse mode: 10 cycles of 20 sec. each.
Ma'lumotnoma/tadqiqot maqolasi:
Kang, H. Ch. et al (2010): PIAS1 regulates CP2c localization and active promoter complex formation in erythroid cell-specific a-globin expression. Nucleic Acids Res. 38/ 16, 2010. pp. 5456–5471.
The lysate of MEL DS19 cells was sonicated with 10 rounds of 20 s each at 70% of maximum output using the Hielscher 200W ultrasonic processor UP200H
Qurilma tavsiyasi:
UP200H; 70% of maximum output; pulse mode: 10 cycles of 20 sec. each.
Ma'lumotnoma/tadqiqot maqolasi:
Kang, H. Ch. et al (2010): PIAS1 regulates CP2c localization and active promoter complex formation in erythroid cell-specific a-globin expression. Nucleic Acids Res. 38/ 16, 2010. pp. 5456–5471.
xromatografiya
Ultrasonik dastur:
Sonication of the adsorbant in the solvent eliminates agglomerates within a few seconds and prepares a uniform, easily packed column. Relevant for adsorbent preparation (e.g. silica gel) prior to column chromatography.
Qurilma tavsiyasi:
UP400S
Sonication of the adsorbant in the solvent eliminates agglomerates within a few seconds and prepares a uniform, easily packed column. Relevant for adsorbent preparation (e.g. silica gel) prior to column chromatography.
Qurilma tavsiyasi:
UP400S
Cladocerans
Ultrasonik dastur:
Cell disruption of mesozooplankton
Qurilma tavsiyasi:
UP2000hd with flow cell
Ma'lumotnoma/tadqiqot maqolasi:
Viitaslo et al. (2005): Ozone, Ultraviolet Light, Ultrasound and Hydrogen Peroxide As Ballast Water Treatments – Experiments with Mesozooplankton In Low Saline- Brackish Water.
Cell disruption of mesozooplankton
Qurilma tavsiyasi:
UP2000hd with flow cell
Ma'lumotnoma/tadqiqot maqolasi:
Viitaslo et al. (2005): Ozone, Ultraviolet Light, Ultrasound and Hydrogen Peroxide As Ballast Water Treatments – Experiments with Mesozooplankton In Low Saline- Brackish Water.
Copepod adults and copepodites
Ultrasonik dastur:
Cell disruption of mesozooplankton: by sonication under following conditions of four flow rates (200, 400, 520 and 800 lh-1) and four amplitudes (25, 50, 75 and 100%) a killing rate between 87 and 99% has been achieved.
Qurilma tavsiyasi:
UIP2000hd with flow cell
Ma'lumotnoma/tadqiqot maqolasi:
Viitaslo et al. (2005): Ozone, Ultraviolet Light, Ultrasound and Hydrogen Peroxide As Ballast Water Treatments – Experiments with Mesozooplankton In Low Saline- Brackish Water.
Cell disruption of mesozooplankton: by sonication under following conditions of four flow rates (200, 400, 520 and 800 lh-1) and four amplitudes (25, 50, 75 and 100%) a killing rate between 87 and 99% has been achieved.
Qurilma tavsiyasi:
UIP2000hd with flow cell
Ma'lumotnoma/tadqiqot maqolasi:
Viitaslo et al. (2005): Ozone, Ultraviolet Light, Ultrasound and Hydrogen Peroxide As Ballast Water Treatments – Experiments with Mesozooplankton In Low Saline- Brackish Water.
Copepod nauplii
Ultrasonik dastur:
Cell disruption of mesozooplankton: by sonication under following conditions of four flow rates (200, 400, 520 and 800 lh-1) and four amplitudes (25, 50, 75 and 100%) a killing rate between 87 and 99% has been achieved.
Qurilma tavsiyasi:
UIP2000hd with flow cell
Ma'lumotnoma/tadqiqot maqolasi:
Viitaslo et al. (2005): Ozone, Ultraviolet Light, Ultrasound and Hydrogen Peroxide As Ballast Water Treatments – Experiments with Mesozooplankton In Low Saline- Brackish Water.
Cell disruption of mesozooplankton: by sonication under following conditions of four flow rates (200, 400, 520 and 800 lh-1) and four amplitudes (25, 50, 75 and 100%) a killing rate between 87 and 99% has been achieved.
Qurilma tavsiyasi:
UIP2000hd with flow cell
Ma'lumotnoma/tadqiqot maqolasi:
Viitaslo et al. (2005): Ozone, Ultraviolet Light, Ultrasound and Hydrogen Peroxide As Ballast Water Treatments – Experiments with Mesozooplankton In Low Saline- Brackish Water.
COS7-cells
Ultrasonik dastur:
Lysis in 400 μl buffer
Qurilma tavsiyasi:
UP200S; 7 cycles
Ma'lumotnoma/tadqiqot maqolasi:
Zaim (2005): Analyse von Nesprin-2 Defizienten Mäusen.
Lysis in 400 μl buffer
Qurilma tavsiyasi:
UP200S; 7 cycles
Ma'lumotnoma/tadqiqot maqolasi:
Zaim (2005): Analyse von Nesprin-2 Defizienten Mäusen.
Cryptosporidium parvaum
Ultrasonik dastur:
Ultrasonic inactivation of Cryptosporidium parvaum (protozoa) in water.
Qurilma tavsiyasi:
UP400S
Ma'lumotnoma/tadqiqot maqolasi:
Tsukamoto, I.; Yim, B.; Stavarache, C. E.; Furuta, M.; Hashiba, K.; Maeda, Y. (2004): Inactivation of Saccharomyces cerevisiae by ultrasonic irradiation. Ultrasonics Sonochemistry 11/2004. pp. 61–65.
Ultrasonic inactivation of Cryptosporidium parvaum (protozoa) in water.
Qurilma tavsiyasi:
UP400S
Ma'lumotnoma/tadqiqot maqolasi:
Tsukamoto, I.; Yim, B.; Stavarache, C. E.; Furuta, M.; Hashiba, K.; Maeda, Y. (2004): Inactivation of Saccharomyces cerevisiae by ultrasonic irradiation. Ultrasonics Sonochemistry 11/2004. pp. 61–65.
Cubosomes
Ultrasonik dastur:
Cubosomes – either empty or doped with fluorophore molecules – were prepared by dispersing the appropriate amount of monoolein in a solution of Pluronic F108 using the ultrasonicator UP100H. To obtain fluorescent cubosomes, the fluorophore was dispersed in the melted monoolein by gentle sonification before dispersion in Pluronic F108. The same procedure was followed when the fluorescent cubosomes were also loaded with quercetin.
Sample: sample size: 4 mL – approx. 96.4 wt% of water, 3.3 wt % of monoolein, 0.3 wt% of Pluronic F108. The percentage of the fluorophore was 2.5 × 10−3 and 2.8 × 10−3 wt%. Amount of added quercetin: 6.4 × 10−6 wt%.
Sonikatsiya: UP100H, amplitude 90%, pulse cycle 0.9, for 10 min.
Qurilma tavsiyasi:
UP100H
Ma'lumotnoma/tadqiqot maqolasi:
Murgia, S.; Bonacchi,S.; Falchi, A. M.; Lampis, S.; Lippolis, V.; Meli, V.; Monduzzi, M.; Prodi, L.; Schmidt, J.; Talmon, Y.; Caltagirone, C. (2013): Drug-Loaded Fluorescent Cubosomes: Versatile Nanoparticles for Potential Theranostic Applications. Langmuir 29, 2013. 6673-6679.
Cubosomes – either empty or doped with fluorophore molecules – were prepared by dispersing the appropriate amount of monoolein in a solution of Pluronic F108 using the ultrasonicator UP100H. To obtain fluorescent cubosomes, the fluorophore was dispersed in the melted monoolein by gentle sonification before dispersion in Pluronic F108. The same procedure was followed when the fluorescent cubosomes were also loaded with quercetin.
Sample: sample size: 4 mL – approx. 96.4 wt% of water, 3.3 wt % of monoolein, 0.3 wt% of Pluronic F108. The percentage of the fluorophore was 2.5 × 10−3 and 2.8 × 10−3 wt%. Amount of added quercetin: 6.4 × 10−6 wt%.
Sonikatsiya: UP100H, amplitude 90%, pulse cycle 0.9, for 10 min.
Qurilma tavsiyasi:
UP100H
Ma'lumotnoma/tadqiqot maqolasi:
Murgia, S.; Bonacchi,S.; Falchi, A. M.; Lampis, S.; Lippolis, V.; Meli, V.; Monduzzi, M.; Prodi, L.; Schmidt, J.; Talmon, Y.; Caltagirone, C. (2013): Drug-Loaded Fluorescent Cubosomes: Versatile Nanoparticles for Potential Theranostic Applications. Langmuir 29, 2013. 6673-6679.
Dekkera bruxellensis
Ultrasonik dastur:
Ultrasonic inactivation of Dekkera bruxellensis in water
Qurilma tavsiyasi:
UIP1500hd
Ma'lumotnoma/tadqiqot maqolasi:
Borthwick, K. A. J.; Coakley, W. T.; McDonnell, M. B.; Nowotny, H.; Benes, E.; Grfschl, .M (2005): Development of a novel compact sonicator for cell disruption. J. Microbio. Meths. 60/2005. pp. 207–216. / Lörincz, A. (2004): Ultrasonic cellular disruption of yeast in water-based suspensions. Biosys. Eng. 89/ 2004. pp. 297–308. / Tsukamoto, I.; Yim, B.; Stavarache, C. E.; Furuta, M.; Hashiba, K.; Maeda, Y. (2004): Inactivation of Saccharomyces cerevisiae by ultrasonic irradiation. Ultrason. Sonochem. 11/2004. pp. 61–65.
Ultrasonic inactivation of Dekkera bruxellensis in water
Qurilma tavsiyasi:
UIP1500hd
Ma'lumotnoma/tadqiqot maqolasi:
Borthwick, K. A. J.; Coakley, W. T.; McDonnell, M. B.; Nowotny, H.; Benes, E.; Grfschl, .M (2005): Development of a novel compact sonicator for cell disruption. J. Microbio. Meths. 60/2005. pp. 207–216. / Lörincz, A. (2004): Ultrasonic cellular disruption of yeast in water-based suspensions. Biosys. Eng. 89/ 2004. pp. 297–308. / Tsukamoto, I.; Yim, B.; Stavarache, C. E.; Furuta, M.; Hashiba, K.; Maeda, Y. (2004): Inactivation of Saccharomyces cerevisiae by ultrasonic irradiation. Ultrason. Sonochem. 11/2004. pp. 61–65.
Dekkera/ Brettanomyces bruxellensis
Ultrasonik dastur:
Inactivation in 90-120 sec.
Qurilma tavsiyasi:
UIP1500hd
Ma'lumotnoma/tadqiqot maqolasi:
see above
Inactivation in 90-120 sec.
Qurilma tavsiyasi:
UIP1500hd
Ma'lumotnoma/tadqiqot maqolasi:
see above
DNK
Ultrasonik dastur:
DNA fragmentation: 2 min. sonication of 100μL with UP100H or 4 min. sonication of 100μL with UTR200.
Qurilma tavsiyasi:
UP100H, UTR200 yoki VialTweeter
Ma'lumotnoma/tadqiqot maqolasi:
Larguinho M. et al. (2010): Development of a fast and efficient ultrasonic-based strategy for DNA fragmentation.
DNA fragmentation: 2 min. sonication of 100μL with UP100H or 4 min. sonication of 100μL with UTR200.
Qurilma tavsiyasi:
UP100H, UTR200 yoki VialTweeter
Ma'lumotnoma/tadqiqot maqolasi:
Larguinho M. et al. (2010): Development of a fast and efficient ultrasonic-based strategy for DNA fragmentation.
Drosophila melanogaster S2 cells
Ultrasonik dastur:
Extraction of cellular proteins from Drosophila melanogaster S2 cells: Frozen S2 cells (1.56108) were lysed in 1 mL ice-cold homogenization buffer (100 mM Tris-HCl pH 7.5, 1% SDS) and left on ice for 10 min. Cell lysis was completed by ultrasonication on ice (6615 bursts, 0.5 s pulse; 75% intensity) with a Hielscher UP200S ultrasonic processor.
Qurilma tavsiyasi:
UP200S (200W), 75% intensitypulse mode: 6615 bursts, 0.5 s pulse.
Ma'lumotnoma/tadqiqot maqolasi:
Schwientek, T. et al. (2007): A serial lectin approach to the mucin-type O-glycoproteome of Drosophila melanogaster S2 cells. Proteomics 7, 2007. pp. 3264-3277.
Extraction of cellular proteins from Drosophila melanogaster S2 cells: Frozen S2 cells (1.56108) were lysed in 1 mL ice-cold homogenization buffer (100 mM Tris-HCl pH 7.5, 1% SDS) and left on ice for 10 min. Cell lysis was completed by ultrasonication on ice (6615 bursts, 0.5 s pulse; 75% intensity) with a Hielscher UP200S ultrasonic processor.
Qurilma tavsiyasi:
UP200S (200W), 75% intensitypulse mode: 6615 bursts, 0.5 s pulse.
Ma'lumotnoma/tadqiqot maqolasi:
Schwientek, T. et al. (2007): A serial lectin approach to the mucin-type O-glycoproteome of Drosophila melanogaster S2 cells. Proteomics 7, 2007. pp. 3264-3277.
E. coli derivates
Ultrasonik dastur:
Cell disruption of E. coli derivates: The frozen pellets corresponding to the sample volume of Vculture = 4/OD mL were resuspended in 580 μL 10 mM potassium phosphate buffer pH 7, 1 mM EDTA. 20 μL of lysozyme (concentration of 1 g L-1) was added and the cells suspension was incubated on ice for about 30 min. Afterwards cells were disrupted by continuous ultrasonication with an ultrasonicator (UP 200S Ultraschallprozessor, Dr. Hielscher GmbH, Teltow) on ice, at an amplitude of 50 % for 20 sec. The soluble and insoluble cell fractions were separated by centrifugation at 13000 rpm for 20 min. The insoluble proteins were washed twice with the 10 mM potassium phosphate buffer pH 7, 1 mM EDTA and stored at –20°C.
Qurilma tavsiyasi:
UP200S with 50% amplitude
Ma'lumotnoma/tadqiqot maqolasi:
HA (2005): Optimisation of active recombinant protein production, exploring the impact of small heat-shock proteins of Escherichia coli, IbpA and IbpB, on in vivo reactivation of inclusion bodies.
Cell disruption of E. coli derivates: The frozen pellets corresponding to the sample volume of Vculture = 4/OD mL were resuspended in 580 μL 10 mM potassium phosphate buffer pH 7, 1 mM EDTA. 20 μL of lysozyme (concentration of 1 g L-1) was added and the cells suspension was incubated on ice for about 30 min. Afterwards cells were disrupted by continuous ultrasonication with an ultrasonicator (UP 200S Ultraschallprozessor, Dr. Hielscher GmbH, Teltow) on ice, at an amplitude of 50 % for 20 sec. The soluble and insoluble cell fractions were separated by centrifugation at 13000 rpm for 20 min. The insoluble proteins were washed twice with the 10 mM potassium phosphate buffer pH 7, 1 mM EDTA and stored at –20°C.
Qurilma tavsiyasi:
UP200S with 50% amplitude
Ma'lumotnoma/tadqiqot maqolasi:
HA (2005): Optimisation of active recombinant protein production, exploring the impact of small heat-shock proteins of Escherichia coli, IbpA and IbpB, on in vivo reactivation of inclusion bodies.
Echinococcus granulosus Antigen
Ultrasonik dastur:
Cell lysis and disintegration: The homogenized sample of protein soluble of mature E. granulosus, prepared by freeze-thawing in liquid nitrogen and 42◦C, has been sonicated at 110V, 170W for 3×15 sec on ice. After that, the sample was centrifugated for 15min at 10000g. Protein concentaration was measured by Bradford method and stored at -20◦C.
Qurilma tavsiyasi:
UP200S; 170W, cooling on ice; 3 x 15 sec.
Ma'lumotnoma/tadqiqot maqolasi:
Tabar et al. (2010): Serodiagnosis of Sheep Hydatidosis with Hydatid Fluid, Protoscolex and Whole Body of Echinococcus granulosus Antigen.
Cell lysis and disintegration: The homogenized sample of protein soluble of mature E. granulosus, prepared by freeze-thawing in liquid nitrogen and 42◦C, has been sonicated at 110V, 170W for 3×15 sec on ice. After that, the sample was centrifugated for 15min at 10000g. Protein concentaration was measured by Bradford method and stored at -20◦C.
Qurilma tavsiyasi:
UP200S; 170W, cooling on ice; 3 x 15 sec.
Ma'lumotnoma/tadqiqot maqolasi:
Tabar et al. (2010): Serodiagnosis of Sheep Hydatidosis with Hydatid Fluid, Protoscolex and Whole Body of Echinococcus granulosus Antigen.
Escherchia coli Gr-
Ultrasonik dastur:
Ultrasonic inactivation of Escherchia coli Gr- in milk and juices.
Qurilma tavsiyasi:
UP100H
Ma'lumotnoma/tadqiqot maqolasi:
Zenker, M.; Heinz, V.; Knorr, D. (2003): Application of ultrasound assisted thermal processing for preservation and quality retention of liquid foods. J Food Prot 66/2003. pp. 1642–1649.
Ultrasonic inactivation of Escherchia coli Gr- in milk and juices.
Qurilma tavsiyasi:
UP100H
Ma'lumotnoma/tadqiqot maqolasi:
Zenker, M.; Heinz, V.; Knorr, D. (2003): Application of ultrasound assisted thermal processing for preservation and quality retention of liquid foods. J Food Prot 66/2003. pp. 1642–1649.
Escherchia coli Gr- in saline
Ultrasonik dastur:
Ultrasonic inactivation of Escherchia coli Gr- in saline.
Qurilma tavsiyasi:
UP100H
Ma'lumotnoma/tadqiqot maqolasi:
Duckhouse, H.; Mason, T. J.; Phull, S. S.; Lorimer, J. P. (2004): The effect of sonication on microbial disinfection using hypochlorite. Ultrason. Sonochem. 11/ 2004. pp. 173–176.
Ultrasonic inactivation of Escherchia coli Gr- in saline.
Qurilma tavsiyasi:
UP100H
Ma'lumotnoma/tadqiqot maqolasi:
Duckhouse, H.; Mason, T. J.; Phull, S. S.; Lorimer, J. P. (2004): The effect of sonication on microbial disinfection using hypochlorite. Ultrason. Sonochem. 11/ 2004. pp. 173–176.
Escherchia coli Gr- in water
Ultrasonik dastur:
Ultrasonic inactivation of Escherchia coli Gr- in water.
Qurilma tavsiyasi:
UP100H
Ma'lumotnoma/tadqiqot maqolasi:
Furuta, M.; Yamaguchi, M.; Tsukamoto, T.; Yim, B.; Stavarache, C. E.; Hasiba, K.; Maeda, Y. (2004): Inactivation of Escherichia coli by ultrasonic irradiation. Ultrason. Sonochem. 11/2004. pp. 57–60.
Ultrasonic inactivation of Escherchia coli Gr- in water.
Qurilma tavsiyasi:
UP100H
Ma'lumotnoma/tadqiqot maqolasi:
Furuta, M.; Yamaguchi, M.; Tsukamoto, T.; Yim, B.; Stavarache, C. E.; Hasiba, K.; Maeda, Y. (2004): Inactivation of Escherichia coli by ultrasonic irradiation. Ultrason. Sonochem. 11/2004. pp. 57–60.
Glaucoma, optical nerve head
Ultrasonik dastur:
RNA fragmentation of optical nerve heads (from rat eyes): The optical nerve head (ONH) was frozen on dry ice and stored at 80°C before extraction. RNA was isolated by sonicating the frozen nerve heads in the kit extraction buffer using an MS 0.5 probe (UP50H) and then, after the instructions provided by the Arcturus kit, including DNase treatment to remove any DNA. Purified RNA was quantified.
Qurilma tavsiyasi:
UP50H with probe MS0.5
Ma'lumotnoma/tadqiqot maqolasi:
Johnson et al. (2007): Global Changes in Optic Nerve Head Gene Expression after Exposure to Elevated Intraocular Pressure in a Rat Glaucoma Model.
RNA fragmentation of optical nerve heads (from rat eyes): The optical nerve head (ONH) was frozen on dry ice and stored at 80°C before extraction. RNA was isolated by sonicating the frozen nerve heads in the kit extraction buffer using an MS 0.5 probe (UP50H) and then, after the instructions provided by the Arcturus kit, including DNase treatment to remove any DNA. Purified RNA was quantified.
Qurilma tavsiyasi:
UP50H with probe MS0.5
Ma'lumotnoma/tadqiqot maqolasi:
Johnson et al. (2007): Global Changes in Optic Nerve Head Gene Expression after Exposure to Elevated Intraocular Pressure in a Rat Glaucoma Model.
Glycosaminoglycan chondroitin sulfate (CS)
Ultrasonik dastur:
Determining CS by Dimethylmethylene Blue Assay
Resuspension of cells: CS pellets in microcentrifuge tubes were resuspended in 0.5 ml of a 0.1mg/ml papain solution in Hank’s balanced salt solution (HBSS) using pulses from an ultrasound horn (UP 100H, Hielscher Ultrasonics GmbH, Germany) at cycle 1 and 100 % amplitude for 3 sec. Thereafter, digestion took place at 60 °C for 24 h.
For Enzyme-linked immunosorbent assay (ELISA), the cells were then suspended in distilled and deionized water at a concentration of 106 cells/ml, and were kept in a freezer at –70°C overnight in order to facilitate cell lysis. The cells were then homogenized by ultrasonication for 40 sec. using the Hielscher ultrasonic tissue homogenizer UP100H.
Qurilma tavsiyasi:
UP100H
Ma'lumotnoma/tadqiqot maqolasi:
Vandrovcova et al. (2011): Influence of Collagen and Chondroitin Sulfate (CS) Coatings on Poly-(Lactide-co-Glycolide) (PLGA) on MG 63 Osteoblast-Like Cells. Physiol. Res. 60; 2011. 797-813.
Determining CS by Dimethylmethylene Blue Assay
Resuspension of cells: CS pellets in microcentrifuge tubes were resuspended in 0.5 ml of a 0.1mg/ml papain solution in Hank’s balanced salt solution (HBSS) using pulses from an ultrasound horn (UP 100H, Hielscher Ultrasonics GmbH, Germany) at cycle 1 and 100 % amplitude for 3 sec. Thereafter, digestion took place at 60 °C for 24 h.
For Enzyme-linked immunosorbent assay (ELISA), the cells were then suspended in distilled and deionized water at a concentration of 106 cells/ml, and were kept in a freezer at –70°C overnight in order to facilitate cell lysis. The cells were then homogenized by ultrasonication for 40 sec. using the Hielscher ultrasonic tissue homogenizer UP100H.
Qurilma tavsiyasi:
UP100H
Ma'lumotnoma/tadqiqot maqolasi:
Vandrovcova et al. (2011): Influence of Collagen and Chondroitin Sulfate (CS) Coatings on Poly-(Lactide-co-Glycolide) (PLGA) on MG 63 Osteoblast-Like Cells. Physiol. Res. 60; 2011. 797-813.
HaCaT cells
Ultrasonik dastur:
Lysis of HaCaT cells for Chromatin Immunoprecipitation (ChIP): HaCaT cells were fixed with 1% formaldehyde for 10 minutes at 37uC. The fixed cells were lysed in the RIPA buffer and sonicated on ice with an 100W ultrasonic device at amplitude = 1 and duty cycle = 100% in 12 one-minute (12 x 1 min.) pulses.
Qurilma tavsiyasi:
UP100H; for 12 min. on ice,
Ma'lumotnoma/tadqiqot maqolasi:
Zhang et al. (2007): Basonuclin Regulates a Subset of Ribosomal RNA Genes in HaCaT Cells.
Lysis of HaCaT cells for Chromatin Immunoprecipitation (ChIP): HaCaT cells were fixed with 1% formaldehyde for 10 minutes at 37uC. The fixed cells were lysed in the RIPA buffer and sonicated on ice with an 100W ultrasonic device at amplitude = 1 and duty cycle = 100% in 12 one-minute (12 x 1 min.) pulses.
Qurilma tavsiyasi:
UP100H; for 12 min. on ice,
Ma'lumotnoma/tadqiqot maqolasi:
Zhang et al. (2007): Basonuclin Regulates a Subset of Ribosomal RNA Genes in HaCaT Cells.
Heparin: Depolymerization of heparin
Ultrasonik dastur:
The ultrasonic method enables to produce a low molecular weight heparin (LMWH). Therefore, heparin undergoes a hydrogen peroxide-catalyzed radical depolymerization. The reaction is very fast and takes less than 1 hour, whilst the physicochemical depolymerization process relies on mild reaction conditions, requires no harsh or toxic reagents, and provides a product free of chemical artifacts. Thus, the ultrasonic process is well suited for the large scale production of LMWH, but also analogs produced from natural sulfated polysaccharides.
Ultrasonic Procedure:
For the physicochemical depolymerization of unfractionated heparin by ultrasonic-assisted radical depolymerization, heparin was dissolved in water to a final concentration of 25 mg/mL (5 mL). The reaction mixture was sonicated using a probe-type ultrasonicator UP50H. The ultrasonicator was equipped with a probe (micro tip MS3) with a diameter of 3mm, which provided an amplitude of 180μm. The processor generated mechanical longitudinal vibrations with a frequency of 30 kHz that were produced by electrical excitation. The reaction mixture was maintained at 60°C in a Radleys® reactor and ultrasonic waves were applied as a 0.5sec pulse to prevent mixture heating. A zerotime aliquot (250μl) was removed from the solution prior to reaction launching by simultaneous addition of hydrogen peroxide and application of ultrasonic waves. Hydrogen peroxide was added to obtain a final hydrogen peroxide/heparin (w/w) ratio of 0.15 (3.75 mg/mL).
Qurilma tavsiyasi:
UP50H with sonotrode MS3
Ma'lumotnoma/tadqiqot maqolasi:
Achour, Oussama; Bridiau, Nicolas; Godhbani, Azza; Le Joubioux, Florian; Bordenave Juchereau, Stephanie; Sannier, Fredéric; Piot, Jean-Marie; Fruitier Arnaudin, Ingrid; Maugard, Thierry (2013): Ultrasonic-assisted preparation of a low molecular weight heparin (LMWH) with anticoagulant activity. Carbohydrate Polymers 97; 2013. 684–689.
The ultrasonic method enables to produce a low molecular weight heparin (LMWH). Therefore, heparin undergoes a hydrogen peroxide-catalyzed radical depolymerization. The reaction is very fast and takes less than 1 hour, whilst the physicochemical depolymerization process relies on mild reaction conditions, requires no harsh or toxic reagents, and provides a product free of chemical artifacts. Thus, the ultrasonic process is well suited for the large scale production of LMWH, but also analogs produced from natural sulfated polysaccharides.
Ultrasonic Procedure:
For the physicochemical depolymerization of unfractionated heparin by ultrasonic-assisted radical depolymerization, heparin was dissolved in water to a final concentration of 25 mg/mL (5 mL). The reaction mixture was sonicated using a probe-type ultrasonicator UP50H. The ultrasonicator was equipped with a probe (micro tip MS3) with a diameter of 3mm, which provided an amplitude of 180μm. The processor generated mechanical longitudinal vibrations with a frequency of 30 kHz that were produced by electrical excitation. The reaction mixture was maintained at 60°C in a Radleys® reactor and ultrasonic waves were applied as a 0.5sec pulse to prevent mixture heating. A zerotime aliquot (250μl) was removed from the solution prior to reaction launching by simultaneous addition of hydrogen peroxide and application of ultrasonic waves. Hydrogen peroxide was added to obtain a final hydrogen peroxide/heparin (w/w) ratio of 0.15 (3.75 mg/mL).
Qurilma tavsiyasi:
UP50H with sonotrode MS3
Ma'lumotnoma/tadqiqot maqolasi:
Achour, Oussama; Bridiau, Nicolas; Godhbani, Azza; Le Joubioux, Florian; Bordenave Juchereau, Stephanie; Sannier, Fredéric; Piot, Jean-Marie; Fruitier Arnaudin, Ingrid; Maugard, Thierry (2013): Ultrasonic-assisted preparation of a low molecular weight heparin (LMWH) with anticoagulant activity. Carbohydrate Polymers 97; 2013. 684–689.
hops
Ultrasonik dastur:
Extraction of active compounds / herbal extracts in water and ethanol.
Qurilma tavsiyasi:
UP400S
Extraction of active compounds / herbal extracts in water and ethanol.
Qurilma tavsiyasi:
UP400S
Lactobacillus (lysis/ DNA isolation of various Lactobacillus strains)
Ultrasonik dastur:
Lactobacillus strains: L. pontis, L. sanfranciscensis, L. farciminis, L. panis, L. oris, L. vaginalis and L. reuteri, L. sp.
Procedure: For the DNA isolation of single colonies, an ultrasonic lysis protocol was developed. One colony (2- to 3-mm diameter) was suspended in 100μl lysis buffer (20 mM EDTA, 10 mM Tris [pH 7.9], 1% Triton X-100, 500 mM guanidine-HCl, 250 mM NaCl). Cells were lysed by 1 min of ultrasonication with an probe-type ultrasonicator UP50H. After the addition of 150μl of cold ethanol (-20°C), the mixture was centrifuged over a spin column of the QIAamp tissue kit and finally eluted with 60μl of buffer (10 mM Tris [pH 7,5]).
To have a tool for a fast and reliable identification of single pure cultures, the PCR assay was combined with a fast DNA isolation procedure. Time-consuming enzymatic lysis procedures and variable susceptibility of bacteria to the lysozyme were overcome by ultrasonic treatment of the cells, with subsequent purification and concentration by binding DNA to a silica matrix. The cell material of a single colony was found to be sufficient for the PCR.
Qurilma tavsiyasi:
UP50H
Ma'lumotnoma/tadqiqot maqolasi:
Müller, M. R. A. (2000): Characterization of the Microbial Ecosystem of Cereal Fermentations using Molecular Biological Methods. Dissertation University of Cologne, 2000.
Lactobacillus strains: L. pontis, L. sanfranciscensis, L. farciminis, L. panis, L. oris, L. vaginalis and L. reuteri, L. sp.
Procedure: For the DNA isolation of single colonies, an ultrasonic lysis protocol was developed. One colony (2- to 3-mm diameter) was suspended in 100μl lysis buffer (20 mM EDTA, 10 mM Tris [pH 7.9], 1% Triton X-100, 500 mM guanidine-HCl, 250 mM NaCl). Cells were lysed by 1 min of ultrasonication with an probe-type ultrasonicator UP50H. After the addition of 150μl of cold ethanol (-20°C), the mixture was centrifuged over a spin column of the QIAamp tissue kit and finally eluted with 60μl of buffer (10 mM Tris [pH 7,5]).
To have a tool for a fast and reliable identification of single pure cultures, the PCR assay was combined with a fast DNA isolation procedure. Time-consuming enzymatic lysis procedures and variable susceptibility of bacteria to the lysozyme were overcome by ultrasonic treatment of the cells, with subsequent purification and concentration by binding DNA to a silica matrix. The cell material of a single colony was found to be sufficient for the PCR.
Qurilma tavsiyasi:
UP50H
Ma'lumotnoma/tadqiqot maqolasi:
Müller, M. R. A. (2000): Characterization of the Microbial Ecosystem of Cereal Fermentations using Molecular Biological Methods. Dissertation University of Cologne, 2000.
Lactobacillus acidophilus Gr+
Ultrasonik dastur:
Ultrasonic inactivation of Lactobacillus acidophilus Gr+ in milk and juices.
Qurilma tavsiyasi:
UIP500hd
Ma'lumotnoma/tadqiqot maqolasi:
Zenker, M.; Heinz, V.; Knorr, D. (2003): Application of ultrasound assisted thermal processing for preservation and quality retention of liquid foods. J Food Prot 66/2003. pp. 1642–1649.
Ultrasonic inactivation of Lactobacillus acidophilus Gr+ in milk and juices.
Qurilma tavsiyasi:
UIP500hd
Ma'lumotnoma/tadqiqot maqolasi:
Zenker, M.; Heinz, V.; Knorr, D. (2003): Application of ultrasound assisted thermal processing for preservation and quality retention of liquid foods. J Food Prot 66/2003. pp. 1642–1649.
Legionella pneumophila Gr+ in diluted medium
Ultrasonik dastur:
Ultrasonic inactivation of Legionella pneumophila Gr+ in diluted medium.
Qurilma tavsiyasi:
UIP500hd
Ma'lumotnoma/tadqiqot maqolasi:
Dadjour, M. F.; Ogino, C.; Matsumura, S.; Nakamura, S.; Shimizu N. (2006): Disinfection of Legionella pneumophila by ultrasonic treatment with TiO2. Water Res 40/2006. pp.1137–1142.
Ultrasonic inactivation of Legionella pneumophila Gr+ in diluted medium.
Qurilma tavsiyasi:
UIP500hd
Ma'lumotnoma/tadqiqot maqolasi:
Dadjour, M. F.; Ogino, C.; Matsumura, S.; Nakamura, S.; Shimizu N. (2006): Disinfection of Legionella pneumophila by ultrasonic treatment with TiO2. Water Res 40/2006. pp.1137–1142.
Leuconostoc mesenteroides
Ultrasonik dastur:
Leukocyte lysozme activity in myelocytic leukemia: the cell suspension was sonicated for 15 min. and samples assayed for lysozyme activity. The lysozyme concentration of the leukocytes ug./10 cells were determined.
Qurilma tavsiyasi:
UP50H
Leukocyte lysozme activity in myelocytic leukemia: the cell suspension was sonicated for 15 min. and samples assayed for lysozyme activity. The lysozyme concentration of the leukocytes ug./10 cells were determined.
Qurilma tavsiyasi:
UP50H
Leukocytic isozym activity in myelocytic leukemia
Ultrasonik dastur:
Sample preparation: cell suspension was ultrasonically treated and samples assayed for lysozyme activity. The lysozyme concentration of the leukocytes ug/106 was determined.
Qurilma tavsiyasi:
UP200St
Sample preparation: cell suspension was ultrasonically treated and samples assayed for lysozyme activity. The lysozyme concentration of the leukocytes ug/106 was determined.
Qurilma tavsiyasi:
UP200St
lipozomalar
Ultrasonik dastur:
Formation of SUVs
Ultrasonik lipozoma tayyorlash haqida ko'proq o'qish uchun shu yerni bosing!
Qurilma tavsiyasi:
UP50H; 3 cycles of 5 min; in an ice bath.
Formation of SUVs
Ultrasonik lipozoma tayyorlash haqida ko'proq o'qish uchun shu yerni bosing!
Qurilma tavsiyasi:
UP50H; 3 cycles of 5 min; in an ice bath.
Yashil malaxit
Ultrasonik dastur:
Sonophotocatalytic degradation of Malachite Green (a strong bactericide): The photocatalytic degradation alone is considerably faster than sonolytic degradation, efficiency can be improved by coupling the two processes. Malachite green, a strong bactericide, is very ecotoxic to marine bacteria but it is converted to organics that are less or not toxic.
Qurilma tavsiyasi:
UP400S
Sonophotocatalytic degradation of Malachite Green (a strong bactericide): The photocatalytic degradation alone is considerably faster than sonolytic degradation, efficiency can be improved by coupling the two processes. Malachite green, a strong bactericide, is very ecotoxic to marine bacteria but it is converted to organics that are less or not toxic.
Qurilma tavsiyasi:
UP400S
Mangiferin acylation
Ultrasonik dastur:
Mangiferin (1,3,6,7-Tetragidroksi-2-[3,4,5-trigidroksi-6-(gidroksimetil)oksan-2-il]ksanten-9-bir; formula: C19H18O11)is a polyphenol of C-glycosylxanthone structure that can be found in many plant species. Mangiferin shows various pharmacological activities. The regioselective acylation of mangiferin can be very efficiently catalyzed by lipase under ultrasonication. Compared with the conventional methods, the ultrasonically-assisted catalysis excels by the advantages of shorter reaction time and higher yields. The optimum conditions for the ultrasonic mangiferin acylation were found as following:
lipaz: PCL, asil donor: vinil asetat; reaksiya erituvchisi: DMSO, reaksiya harorati: 45 degC, ultratovush quvvati: 200W; substrat nisbati: asil donor/mangiferin 6/1, ferment yuklanishi: 6 mg/ml
Regioselektiv asillanish rentabelligi 84% gacha edi.
Qurilma tavsiyasi:
UP200St yoki UP200Ht
Ma'lumotnoma/tadqiqot maqolasi:
cp.: Wang, Z.; Wang, R.; Tian, J.; Zhao, B; Wei, X.F.; Su, Y.L.; Li, C.Y.; Cao, S.G.; Wang, L. (2010): The effect of ultrasound on lipase-catalyzed regioselective acylation of mangiferin in non-aqueous solvents. J. Asian Nat Prod. Res. 12/1, 2010. 56-63.
Mangiferin (1,3,6,7-Tetragidroksi-2-[3,4,5-trigidroksi-6-(gidroksimetil)oksan-2-il]ksanten-9-bir; formula: C19H18O11)is a polyphenol of C-glycosylxanthone structure that can be found in many plant species. Mangiferin shows various pharmacological activities. The regioselective acylation of mangiferin can be very efficiently catalyzed by lipase under ultrasonication. Compared with the conventional methods, the ultrasonically-assisted catalysis excels by the advantages of shorter reaction time and higher yields. The optimum conditions for the ultrasonic mangiferin acylation were found as following:
lipaz: PCL, asil donor: vinil asetat; reaksiya erituvchisi: DMSO, reaksiya harorati: 45 degC, ultratovush quvvati: 200W; substrat nisbati: asil donor/mangiferin 6/1, ferment yuklanishi: 6 mg/ml
Regioselektiv asillanish rentabelligi 84% gacha edi.
Qurilma tavsiyasi:
UP200St yoki UP200Ht
Ma'lumotnoma/tadqiqot maqolasi:
cp.: Wang, Z.; Wang, R.; Tian, J.; Zhao, B; Wei, X.F.; Su, Y.L.; Li, C.Y.; Cao, S.G.; Wang, L. (2010): The effect of ultrasound on lipase-catalyzed regioselective acylation of mangiferin in non-aqueous solvents. J. Asian Nat Prod. Res. 12/1, 2010. 56-63.
Molecules extraction
Ultrasonik dastur:
Extraction protocol for extraction from gypsum, rheolite, basalt, edfell ash and obsidian glass:
A 1g sample of regolith or crushed rock is subject to liquid extraction under ultrasonic irradiation to extract and solubilise target molecules. Therefore, 3ml MeOH P80 was added to 1g analogue sample in a glass test tube and sonicated for 20 minutes with an ultrasonic probe-type device UP50H set at 40% amplitude. The mixture was allowed to stand for 10 minutes, and the cloudy supernatant that had formed above the sedimented analogue sample was decanted into 1.5 ml centrifuge tubes. To minimize loss of extracted components by adsorption to surfaces of the hydrophobic polymer centrifuge tubes, the tubes were first blocked with 0.5% (w/v) BSA in 100 mM HEPES pH 7.4. The supernatants were clarified by centrifugation at 17000 G for 10 minutes and stored at 2 – 8°C in glass vials until tested in the immunoassay.
Qurilma tavsiyasi:
UP50H
Ma'lumotnoma/tadqiqot maqolasi:
Rix, C.(2012): Detecting Life on Mars and the Life Marker Chip: Antibody Assays for Detecting Organic Molecules in Liquid Extracts of Martian Samples. Dissertation Cranfield University 2012.
Extraction protocol for extraction from gypsum, rheolite, basalt, edfell ash and obsidian glass:
A 1g sample of regolith or crushed rock is subject to liquid extraction under ultrasonic irradiation to extract and solubilise target molecules. Therefore, 3ml MeOH P80 was added to 1g analogue sample in a glass test tube and sonicated for 20 minutes with an ultrasonic probe-type device UP50H set at 40% amplitude. The mixture was allowed to stand for 10 minutes, and the cloudy supernatant that had formed above the sedimented analogue sample was decanted into 1.5 ml centrifuge tubes. To minimize loss of extracted components by adsorption to surfaces of the hydrophobic polymer centrifuge tubes, the tubes were first blocked with 0.5% (w/v) BSA in 100 mM HEPES pH 7.4. The supernatants were clarified by centrifugation at 17000 G for 10 minutes and stored at 2 – 8°C in glass vials until tested in the immunoassay.
Qurilma tavsiyasi:
UP50H
Ma'lumotnoma/tadqiqot maqolasi:
Rix, C.(2012): Detecting Life on Mars and the Life Marker Chip: Antibody Assays for Detecting Organic Molecules in Liquid Extracts of Martian Samples. Dissertation Cranfield University 2012.
Mouse liver pellets
Ultrasonik dastur:
The pellets were washed and sonicated for 5 min with a further 0.5 mL of LB2 and centrifuged at 12,000 g for another 20 min, and the resulting two fractions of supernatant were collected. Finally, the pellets were dissolved with 0.5 mL of buffer containing 40 mM tris base, 5 M urea, 2 M thiourea, 4% CHAPS, 100 mM DTT, 0.5% (v/v) biolyte 3-10 (LB3), and were sonicated and centrifuged at 12,000 g for 20 min.
Qurilma tavsiyasi:
UP200S; for 5min.
Ma'lumotnoma/tadqiqot maqolasi:
Gazzana et al. (2009): An update on mouse liver proteome.
The pellets were washed and sonicated for 5 min with a further 0.5 mL of LB2 and centrifuged at 12,000 g for another 20 min, and the resulting two fractions of supernatant were collected. Finally, the pellets were dissolved with 0.5 mL of buffer containing 40 mM tris base, 5 M urea, 2 M thiourea, 4% CHAPS, 100 mM DTT, 0.5% (v/v) biolyte 3-10 (LB3), and were sonicated and centrifuged at 12,000 g for 20 min.
Qurilma tavsiyasi:
UP200S; for 5min.
Ma'lumotnoma/tadqiqot maqolasi:
Gazzana et al. (2009): An update on mouse liver proteome.
Mouse liver suspension
Ultrasonik dastur:
Sample homogenization to produce cell lysate.
Qurilma tavsiyasi:
UP200S; for 3 x 20 sec.
Ma'lumotnoma/tadqiqot maqolasi:
Gazzana et al. (2009): An update on mouse liver proteome.
Sample homogenization to produce cell lysate.
Qurilma tavsiyasi:
UP200S; for 3 x 20 sec.
Ma'lumotnoma/tadqiqot maqolasi:
Gazzana et al. (2009): An update on mouse liver proteome.
Penicillium digitatum
Ultrasonik dastur:
Ultrasonic inactivation of Penicillium digitatum (a plant pathogen) in Sabouraud growth medium.
Qurilma tavsiyasi:
UP200St
Ma'lumotnoma/tadqiqot maqolasi:
López-Malo, A.; Palou, E.; Jiménez-Fernández, M.; Alzamora, S. M.; Guerrero, S. (2005): Multifactorial fungal inactivation combining thermosonication and antimicrobials. J. Food Eng. 67/ 2005. pp. 87–93.
Ultrasonic inactivation of Penicillium digitatum (a plant pathogen) in Sabouraud growth medium.
Qurilma tavsiyasi:
UP200St
Ma'lumotnoma/tadqiqot maqolasi:
López-Malo, A.; Palou, E.; Jiménez-Fernández, M.; Alzamora, S. M.; Guerrero, S. (2005): Multifactorial fungal inactivation combining thermosonication and antimicrobials. J. Food Eng. 67/ 2005. pp. 87–93.
Phycocyanin from Spirulina platensis (Arthrospira platensis)
Ultrasonik dastur:
Extraction of phycocyanin from Spirulina platensis (Arthrospira platensis) cells.
Qurilma tavsiyasi:
UP400S
Extraction of phycocyanin from Spirulina platensis (Arthrospira platensis) cells.
Qurilma tavsiyasi:
UP400S
Plant cells and plant tissue
Ultrasonik dastur:
30% packed plant cells (W/V) and distilled water are disrupted by sonication for 1-15 min.; plant tissue disintegration: 1 g dried tissue suspended in alcohol is disintegrated during sonication of about 5 min.
Qurilma tavsiyasi:
UP100H
30% packed plant cells (W/V) and distilled water are disrupted by sonication for 1-15 min.; plant tissue disintegration: 1 g dried tissue suspended in alcohol is disintegrated during sonication of about 5 min.
Qurilma tavsiyasi:
UP100H
trombotsitlar
Pleurotus tuberregium
Ultrasonik dastur:
Extraction of polysaccharides from the edible fungus Pleurotus tuberregium
Qurilma tavsiyasi:
UP400S
Extraction of polysaccharides from the edible fungus Pleurotus tuberregium
Qurilma tavsiyasi:
UP400S
Poly(lactic-co-glycolic acid) (PLGA)
Ultrasonik dastur:
Preparation of bovine serum albumin (BSA)-loaded poly(lactic-co-glycolic acid) (PLGA)by sonication in 40 sec.
Qurilma tavsiyasi:
Dmini; for 40sec.
Ma'lumotnoma/tadqiqot maqolasi:
Freitas et al. (2005): Flow-through ultrasonic emulsification combined with static micromixing for aseptic production of microspheres by solvent extraction.
Preparation of bovine serum albumin (BSA)-loaded poly(lactic-co-glycolic acid) (PLGA)by sonication in 40 sec.
Qurilma tavsiyasi:
Dmini; for 40sec.
Ma'lumotnoma/tadqiqot maqolasi:
Freitas et al. (2005): Flow-through ultrasonic emulsification combined with static micromixing for aseptic production of microspheres by solvent extraction.
Polyphenols from apple
Ultrasonik dastur:
Ultrasonic extraction of polyphenols from apple. Solvent: aqueous. Increased yield by 6%; sonication intensity: 20-75Ws/ml; process temp.: heated to 80 degC.
Qurilma tavsiyasi:
UIP2000hd
Ma'lumotnoma/tadqiqot maqolasi:
Vilkhu, K.; Manasseh, R.; Mawson, R.; Ashokkumar, M. (2011): Ultrasonic Recovery and Modification of Food ingredients. In: Feng/ Barbosa-Cánovas/ Weiss (2011): Ultrasound Technologies for Food and Bioprocessing. New York: Springer, 2011. pp. 345-368.
Ultrasonic extraction of polyphenols from apple. Solvent: aqueous. Increased yield by 6%; sonication intensity: 20-75Ws/ml; process temp.: heated to 80 degC.
Qurilma tavsiyasi:
UIP2000hd
Ma'lumotnoma/tadqiqot maqolasi:
Vilkhu, K.; Manasseh, R.; Mawson, R.; Ashokkumar, M. (2011): Ultrasonic Recovery and Modification of Food ingredients. In: Feng/ Barbosa-Cánovas/ Weiss (2011): Ultrasound Technologies for Food and Bioprocessing. New York: Springer, 2011. pp. 345-368.
Polyphenols from black tea
Ultrasonik dastur:
Ultrasonic extraction of polyphenols from black tea. Solvent: aqueous. Increased yield by 6-18%; sonication intensity: 8-10Ws/ml; ambient pressure, process temp.: heated to 90 degC.
Qurilma tavsiyasi:
UIP2000hd
Ma'lumotnoma/tadqiqot maqolasi:
Vilkhu, K.; Manasseh, R.; Mawson, R.; Ashokkumar, M. (2011): Ultrasonic Recovery and Modification of Food ingredients. In: Feng/ Barbosa-Cánovas/ Weiss (2011): Ultrasound Technologies for Food and Bioprocessing. New York: Springer, 2011. pp. 345-368.
Ultrasonic extraction of polyphenols from black tea. Solvent: aqueous. Increased yield by 6-18%; sonication intensity: 8-10Ws/ml; ambient pressure, process temp.: heated to 90 degC.
Qurilma tavsiyasi:
UIP2000hd
Ma'lumotnoma/tadqiqot maqolasi:
Vilkhu, K.; Manasseh, R.; Mawson, R.; Ashokkumar, M. (2011): Ultrasonic Recovery and Modification of Food ingredients. In: Feng/ Barbosa-Cánovas/ Weiss (2011): Ultrasound Technologies for Food and Bioprocessing. New York: Springer, 2011. pp. 345-368.
Polyphenols from red grape marc
Ultrasonik dastur:
Ultrasonic extraction of polyphenols from red grape marc. Solvent: aqueous. Increased yield by 11-35%; sonication intensity: 20-75Ws/ml; ambient pressure.
Qurilma tavsiyasi:
UIP2000hd
Ma'lumotnoma/tadqiqot maqolasi:
Vilkhu, K.; Manasseh, R.; Mawson, R.; Ashokkumar, M. (2011): Ultrasonic Recovery and Modification of Food ingredients. In: Feng/ Barbosa-Cánovas/ Weiss (2011): Ultrasound Technologies for Food and Bioprocessing. New York: Springer, 2011. pp. 345-368.
Ultrasonic extraction of polyphenols from red grape marc. Solvent: aqueous. Increased yield by 11-35%; sonication intensity: 20-75Ws/ml; ambient pressure.
Qurilma tavsiyasi:
UIP2000hd
Ma'lumotnoma/tadqiqot maqolasi:
Vilkhu, K.; Manasseh, R.; Mawson, R.; Ashokkumar, M. (2011): Ultrasonic Recovery and Modification of Food ingredients. In: Feng/ Barbosa-Cánovas/ Weiss (2011): Ultrasound Technologies for Food and Bioprocessing. New York: Springer, 2011. pp. 345-368.
Polyphenols, amino acid and caffeine from green tea
Pork: Brining of pork loins
Ultrasonik dastur:
For ultrasonically assisted brining, pork loins (Longissimus dorsi) were immersed in sodium chloride brine (40 g L−1) and treated at 5°C with low-frequency ultrasound (20 kHz) at low-intensity (2–4 W/cm−2). The effect of the ultrasonic assisted curing on porcine tissue microstructure, protein denaturation, water-binding capacity (WBC), water-holding capacity (WHC), sodium chloride diffusion coefficient (D) and on meat textural profile (TPA) was investigated. Results showed that the ultrasonic treatment caused favourable microstructural changes in meat tissue. Water-holding capacity and textural properties were improved by ultrasonic treatment compared to both tumbled and static brined samples. However, these positive effects were highly dependent on ultrasonic intensity. Higher intensities and/or longer treatment times caused denaturation of proteins. The constant diffusion coefficient model was able to describe precisely the NaCl diffusion kinetics during brining. Ultrasonic treatment significantly enhanced the diffusion of salt compared to samples brined under static conditions and diffusion coefficient exponentially increased with increased ultrasonic intensity.
Qurilma tavsiyasi:
UP200Ht with sonotrode S26d40
Ma'lumotnoma/tadqiqot maqolasi:
Siro, I.; Ven, Cs.; Balla, Cs.; Jonas, G.; Zeke, I.; Friedrich, L. (2009): Application of an ultrasonic assisted curing technique for improving the diffusion of sodium chloride in porcine meat. Journal of Food Engineering 91/2, 2009. 353–362.
For ultrasonically assisted brining, pork loins (Longissimus dorsi) were immersed in sodium chloride brine (40 g L−1) and treated at 5°C with low-frequency ultrasound (20 kHz) at low-intensity (2–4 W/cm−2). The effect of the ultrasonic assisted curing on porcine tissue microstructure, protein denaturation, water-binding capacity (WBC), water-holding capacity (WHC), sodium chloride diffusion coefficient (D) and on meat textural profile (TPA) was investigated. Results showed that the ultrasonic treatment caused favourable microstructural changes in meat tissue. Water-holding capacity and textural properties were improved by ultrasonic treatment compared to both tumbled and static brined samples. However, these positive effects were highly dependent on ultrasonic intensity. Higher intensities and/or longer treatment times caused denaturation of proteins. The constant diffusion coefficient model was able to describe precisely the NaCl diffusion kinetics during brining. Ultrasonic treatment significantly enhanced the diffusion of salt compared to samples brined under static conditions and diffusion coefficient exponentially increased with increased ultrasonic intensity.
Qurilma tavsiyasi:
UP200Ht with sonotrode S26d40
Ma'lumotnoma/tadqiqot maqolasi:
Siro, I.; Ven, Cs.; Balla, Cs.; Jonas, G.; Zeke, I.; Friedrich, L. (2009): Application of an ultrasonic assisted curing technique for improving the diffusion of sodium chloride in porcine meat. Journal of Food Engineering 91/2, 2009. 353–362.
Porphyra yezoensis
Ultrasonik dastur:
Ultrasonic degradation of polysaccharides from Porphyra yezoensis: 50mL of 1.0 g/100mL porphyra yezoensis polysaccarides (dry weight) solution have been sonicated for 4 hr with an UP400S.
Qurilma tavsiyasi:
UP400S; pulsed ultrasound (cycles: 2 sec on/ 2 sec off) at 20°C.
Ultrasonic degradation of polysaccharides from Porphyra yezoensis: 50mL of 1.0 g/100mL porphyra yezoensis polysaccarides (dry weight) solution have been sonicated for 4 hr with an UP400S.
Qurilma tavsiyasi:
UP400S; pulsed ultrasound (cycles: 2 sec on/ 2 sec off) at 20°C.
kukunlar
Ultrasonik dastur:
Grinding, deagglomeration and dispersion to small, relativley uniform particle size.
Qurilma tavsiyasi:
UP200St
Grinding, deagglomeration and dispersion to small, relativley uniform particle size.
Qurilma tavsiyasi:
UP200St
Rat bones
Rat liver
Ultrasonik dastur:
Disruption and tissue homogenization with an UP400S.
Qurilma tavsiyasi:
UP400S; 3 times for 30 sec.; on ice.
Ma'lumotnoma/tadqiqot maqolasi:
Oh et al. (2003): Acetyl-CoA Carboxylase Gene Is Regulated by Sterol Regulatory Element-binding Protein-1 in Liver. Journal of Biological Chemistry 2003.
Disruption and tissue homogenization with an UP400S.
Qurilma tavsiyasi:
UP400S; 3 times for 30 sec.; on ice.
Ma'lumotnoma/tadqiqot maqolasi:
Oh et al. (2003): Acetyl-CoA Carboxylase Gene Is Regulated by Sterol Regulatory Element-binding Protein-1 in Liver. Journal of Biological Chemistry 2003.
Rat skin
Rawolfia Serpentina
rebaudiozid A
Ultrasonik dastur:
Samples of 10g dry and ground stevia leaves were extracted in 100mL water under continuous stirring (with a magnetic stirrer). The pH value was controlled with 0.01 M pH 7 sodium phosphate. The sample was put in a 150 mL glass beaker and sonicated with an probe-type ultrasonicator (UIP500hd, 20kHz, 500W). The tip of the sonotrode was immersed about 1.5 cm into the slurry of stevia leaves. The ultrasonic device was set for a power output of 350W. A mild sonication treatment of 350 W for 5-10 min. at a constant process temperature of 30°C gave a rebaudioside A yield of 30-34g per 100g sample. After sonication, the extract solution was centrifuged and filtered off through 0.45 μm microporous membrane; the filtrate was taken for total rebaudioside A content analysis. The extraction yield of total rebaudioside A content was analyzed by HPLC.
Solventsiz ultratovush yordamida ekstraksiya natijasida issiqlik ekstraktsiyasi yoki maseratsiya kabi an'anaviy ekstraksiya usullariga nisbatan rebaudiozid A ning yuqori rentabelligi olindi.
Qurilma tavsiyasi:
UIP500hd
Samples of 10g dry and ground stevia leaves were extracted in 100mL water under continuous stirring (with a magnetic stirrer). The pH value was controlled with 0.01 M pH 7 sodium phosphate. The sample was put in a 150 mL glass beaker and sonicated with an probe-type ultrasonicator (UIP500hd, 20kHz, 500W). The tip of the sonotrode was immersed about 1.5 cm into the slurry of stevia leaves. The ultrasonic device was set for a power output of 350W. A mild sonication treatment of 350 W for 5-10 min. at a constant process temperature of 30°C gave a rebaudioside A yield of 30-34g per 100g sample. After sonication, the extract solution was centrifuged and filtered off through 0.45 μm microporous membrane; the filtrate was taken for total rebaudioside A content analysis. The extraction yield of total rebaudioside A content was analyzed by HPLC.
Solventsiz ultratovush yordamida ekstraksiya natijasida issiqlik ekstraktsiyasi yoki maseratsiya kabi an'anaviy ekstraksiya usullariga nisbatan rebaudiozid A ning yuqori rentabelligi olindi.
Qurilma tavsiyasi:
UIP500hd
Rice starch
Ultrasonik dastur:
Disruption, starch isolation, and break of noncovalent bonds between protein and starch in a rice flour slurry (33%) in 20 – 40 min.
Qurilma tavsiyasi:
UP500hd; 20 – 40 min.
Disruption, starch isolation, and break of noncovalent bonds between protein and starch in a rice flour slurry (33%) in 20 – 40 min.
Qurilma tavsiyasi:
UP500hd; 20 – 40 min.
Rotifers
Ultrasonik dastur:
Cell disruption of mesozooplankton: by sonication under following conditions of four flow rates (200, 400, 520 and 800lh-1) and four amplitudes (25, 50, 75 and 100%) a killing rate between 58 and 85% has been achieved.
Qurilma tavsiyasi:
UP2000 with flow cell
Ma'lumotnoma/tadqiqot maqolasi:
Viitaslo et al. (2005): Ozone, ultraviolet light, ultrasound and hydrogen peroxide as ballast water treatments – Experiments with mesozooplankton in low-saline brackish water. Journal of Marine Environmental Engineering, 8(1), 35-55.
Cell disruption of mesozooplankton: by sonication under following conditions of four flow rates (200, 400, 520 and 800lh-1) and four amplitudes (25, 50, 75 and 100%) a killing rate between 58 and 85% has been achieved.
Qurilma tavsiyasi:
UP2000 with flow cell
Ma'lumotnoma/tadqiqot maqolasi:
Viitaslo et al. (2005): Ozone, ultraviolet light, ultrasound and hydrogen peroxide as ballast water treatments – Experiments with mesozooplankton in low-saline brackish water. Journal of Marine Environmental Engineering, 8(1), 35-55.
S. fragilis
saccharomyces cerevisiae
Ultrasonik dastur:
buzilish
Qurilma tavsiyasi:
UP400S
Ma'lumotnoma/tadqiqot maqolasi:
Guerrero, S,; López-Malo, A.; Alzamora, S. M. (2001): Effect of ultrasound on the survival of Saccharomyces cerevisiae: influence of temperature, pH and amplitude. Innov. Food Sci. Emerg Technol. 2/2001. pp. 31–39.
buzilish
Qurilma tavsiyasi:
UP400S
Ma'lumotnoma/tadqiqot maqolasi:
Guerrero, S,; López-Malo, A.; Alzamora, S. M. (2001): Effect of ultrasound on the survival of Saccharomyces cerevisiae: influence of temperature, pH and amplitude. Innov. Food Sci. Emerg Technol. 2/2001. pp. 31–39.
saccharomyces cerevisiae
Ultrasonik dastur:
Ultrasonic inactivation of Saccharomyces cerevisiae in Sabouraud growth medium and in saline.
Qurilma tavsiyasi:
UP400S
Ma'lumotnoma/tadqiqot maqolasi:
Yap, A.; Jiranek, V.; Grbin, P.; Barnes, M.; Bates, D. (2007): Studies on the application of high power ultrasonics for barrel and plank cleaning and disinfection. Austr. NZ Wine Indust. J. 22(3)/ 2007. pp. 96–104.
Ultrasonic inactivation of Saccharomyces cerevisiae in Sabouraud growth medium and in saline.
Qurilma tavsiyasi:
UP400S
Ma'lumotnoma/tadqiqot maqolasi:
Yap, A.; Jiranek, V.; Grbin, P.; Barnes, M.; Bates, D. (2007): Studies on the application of high power ultrasonics for barrel and plank cleaning and disinfection. Austr. NZ Wine Indust. J. 22(3)/ 2007. pp. 96–104.
Saffron, crocus sativus
Ultrasonik dastur:
Extraction of active compounds (flavors and color agents).
Click here to read more about the extraction from saffron!
Qurilma tavsiyasi:
UP50H
Ma'lumotnoma/tadqiqot maqolasi:
Kadkhodaee et al. (2007): Ultrasonic Extraction of Active Compounds from Saffron. Acta Hortic. 739, 2007. 417-425.
Extraction of active compounds (flavors and color agents).
Click here to read more about the extraction from saffron!
Qurilma tavsiyasi:
UP50H
Ma'lumotnoma/tadqiqot maqolasi:
Kadkhodaee et al. (2007): Ultrasonic Extraction of Active Compounds from Saffron. Acta Hortic. 739, 2007. 417-425.
Salmonella Senftenberg 775W Gr-
Ultrasonik dastur:
Ultrasonic inactivation of Salmonella Senftenberg 775W Gr- in McIlvaine citrate-phosphate buffer or nutrient broth.
Qurilma tavsiyasi:
UP100H
Ma'lumotnoma/tadqiqot maqolasi:
Álvarez, I.; Manas, P.; Virto, R.; Condón, S. (2006): Inactivation of Salmonella Senftenberg 775W by ultrasonic waves under pressure at different water activities. Int. J. Food Microbiol. 108/2006. pp. 218–225.
Ultrasonic inactivation of Salmonella Senftenberg 775W Gr- in McIlvaine citrate-phosphate buffer or nutrient broth.
Qurilma tavsiyasi:
UP100H
Ma'lumotnoma/tadqiqot maqolasi:
Álvarez, I.; Manas, P.; Virto, R.; Condón, S. (2006): Inactivation of Salmonella Senftenberg 775W by ultrasonic waves under pressure at different water activities. Int. J. Food Microbiol. 108/2006. pp. 218–225.
Salvia miltiorrhiza bunge
Ultrasonik dastur:
Extraction of biological active compounds: sodium Danshensu and four tanshinones (dihydrotanshione I, tanshinone I, cryptotanshinone and tanshinone IIA).
Qurilma tavsiyasi:
UP100H
Extraction of biological active compounds: sodium Danshensu and four tanshinones (dihydrotanshione I, tanshinone I, cryptotanshinone and tanshinone IIA).
Qurilma tavsiyasi:
UP100H
Salvia Officinalis
Ultrasonik dastur:
Extraction of active compounds from Salvia Officinalis (sage) in less than 2hr.
Qurilma tavsiyasi:
UP50H
Extraction of active compounds from Salvia Officinalis (sage) in less than 2hr.
Qurilma tavsiyasi:
UP50H
sarum
Sheep cystic livers, lungs and positive blood (Echinococcus granulosus antigens)
Ultrasonik dastur:
Sheep cystic livers, lungs and positive blood (Echinococcus granulosus antigens in lambs): The sample was then sonicated for 2 × 15 sec on ice until no intact protoscolices were visible.
Qurilma tavsiyasi:
UP200S; 2 x 15 sec.
Ma'lumotnoma/tadqiqot maqolasi:
Tabar et al. (2009): Antibody response against hydatid fluid, protoscolex and whole body of Echinococcus granulosus antigens in lambs. Journal of Veterinary Research, Volume 10, Issue 3; 2009. 283-288.
Sheep cystic livers, lungs and positive blood (Echinococcus granulosus antigens in lambs): The sample was then sonicated for 2 × 15 sec on ice until no intact protoscolices were visible.
Qurilma tavsiyasi:
UP200S; 2 x 15 sec.
Ma'lumotnoma/tadqiqot maqolasi:
Tabar et al. (2009): Antibody response against hydatid fluid, protoscolex and whole body of Echinococcus granulosus antigens in lambs. Journal of Veterinary Research, Volume 10, Issue 3; 2009. 283-288.
Sorbitant Trioleate, ethanol (as solvent) and Bi-2212 powder
Ultrasonik dastur:
Deagglomerate a slurry containing the dispersant Sorbitant Trioleate, ethanol (as solvent) and Bi-2212 powder.
Qurilma tavsiyasi:
UP200S; for 3 min.
Ma'lumotnoma/tadqiqot maqolasi:
Mora et al. (2009): Fabrication of Superconducting Coatings on Structural Ceramic Tiles.
Deagglomerate a slurry containing the dispersant Sorbitant Trioleate, ethanol (as solvent) and Bi-2212 powder.
Qurilma tavsiyasi:
UP200S; for 3 min.
Ma'lumotnoma/tadqiqot maqolasi:
Mora et al. (2009): Fabrication of Superconducting Coatings on Structural Ceramic Tiles.
Soy isoflavones
Ultrasonik dastur:
ultrasonic extraction of soy isoflavones in water and solvent. Increased yield of up to 15% in extraction efficiency.
Qurilma tavsiyasi:
UP200St
Ma'lumotnoma/tadqiqot maqolasi:
Rostagno et al. (2003), referenced by Vilkhu, K.; Manasseh, R.; Mawson, R.; Ashokkumar, M. (2011): Ultrasonic Recovery and Modification of Food ingredients. In: Feng/ Barbosa-Cánovas/ Weiss (2011): Ultrasound Technologies for Food and Bioprocessing. New York: Springer, 2011. pp. 345-368.
ultrasonic extraction of soy isoflavones in water and solvent. Increased yield of up to 15% in extraction efficiency.
Qurilma tavsiyasi:
UP200St
Ma'lumotnoma/tadqiqot maqolasi:
Rostagno et al. (2003), referenced by Vilkhu, K.; Manasseh, R.; Mawson, R.; Ashokkumar, M. (2011): Ultrasonic Recovery and Modification of Food ingredients. In: Feng/ Barbosa-Cánovas/ Weiss (2011): Ultrasound Technologies for Food and Bioprocessing. New York: Springer, 2011. pp. 345-368.
soya proteini
Ultrasonik dastur:
Ultrasonic extraction of soy protein in water and alkali (sodium hydroxide). Increased yield by 53%. Inline sonication was found more efficient as batch extraction (inline sonication gave a 23% higher yield than batch sonication).
Qurilma tavsiyasi:
UIP1000hd for batch/ beaker and with flow cell reactor for inline sonication.
Ma'lumotnoma/tadqiqot maqolasi:
Moulton and Wang (1982), referenced by Vilkhu, K.; Manasseh, R.; Mawson, R.; Ashokkumar, M. (2011): Ultrasonic Recovery and Modification of Food ingredients. In: Feng/ Barbosa-Cánovas/ Weiss (2011): Ultrasound Technologies for Food and Bioprocessing. New York: Springer, 2011. pp. 345-368.
Ultrasonic extraction of soy protein in water and alkali (sodium hydroxide). Increased yield by 53%. Inline sonication was found more efficient as batch extraction (inline sonication gave a 23% higher yield than batch sonication).
Qurilma tavsiyasi:
UIP1000hd for batch/ beaker and with flow cell reactor for inline sonication.
Ma'lumotnoma/tadqiqot maqolasi:
Moulton and Wang (1982), referenced by Vilkhu, K.; Manasseh, R.; Mawson, R.; Ashokkumar, M. (2011): Ultrasonic Recovery and Modification of Food ingredients. In: Feng/ Barbosa-Cánovas/ Weiss (2011): Ultrasound Technologies for Food and Bioprocessing. New York: Springer, 2011. pp. 345-368.
Sperm heads (human)
Sperm tails (human)
Stevia rebaudiana Bert.
Ultrasonik dastur:
Ultrasonic extraction of stevioside glycoside from samples of 10 g of dry leaves from Stevia rebaudiana with four particles sizes (0.315 mm, 2 mm, 6.3 mm and crushed dry leaves) were mixed with different solvents: distilled water and water/ethanol mixtures (55% and 70%) with different sample weight to solvent volume ratios: 1/10, 1/8, 1/5 (w/v) then exposed to ultrasound at room temperature. Sonication time: < 5 min. Qurilma tavsiyasi:
UP200St
Ultrasonic extraction of stevioside glycoside from samples of 10 g of dry leaves from Stevia rebaudiana with four particles sizes (0.315 mm, 2 mm, 6.3 mm and crushed dry leaves) were mixed with different solvents: distilled water and water/ethanol mixtures (55% and 70%) with different sample weight to solvent volume ratios: 1/10, 1/8, 1/5 (w/v) then exposed to ultrasound at room temperature. Sonication time: < 5 min. Qurilma tavsiyasi:
UP200St
Biz bilan bog'lanish! / Bizdan so'rang!
Ushbu qo'llanmada laboratoriyalarda liziz, hujayra buzilishi, oqsil izolyatsiyasi, DNK va RNK parchalanishi, tahlil va tadqiqotlar kabi namuna tayyorlash vazifalaringiz uchun qaysi turdagi sonikator eng yaxshi ekanligini tushuntiradi. Ilovangiz, namuna hajmi, namuna raqami va o'tkazish qobiliyati uchun ideal sonikator turini tanlang. Hielscher Ultrasonics siz uchun ideal ultratovushli homogenizatorga ega!
Fan va tahlilda hujayra buzilishi va oqsil olish uchun mukammal sonikatorni qanday topish mumkin
High Performance Ultrasonicators for Any Size
Hielscher Ultrasonics cell disruptors and tissue homogenizers are available at any size for any volume. Whether you have to sonicate small sample sizes, mass samples such 96-well plates, mid-size volumes or truckloads per hour, our portfolio offer the ideal ultrasonicator for your application. Compact, hand-held ultrasonic homogenizers are optimal for lab and research, whilst our industrial series covers everything between 0,5kW to 16kW per ultrasonic processor. With the capability of being installed as clusters, with Hielscher ultrasonicators you can process virtually any volume. The robustness of Hielscher’s ultrasonic equipment allows for 24/7 operation at heavy duty and in demanding environments.
The sophisticated and smart software allows for highest process control and operator’s convenience. All sonication data are automatically recorded on a built-in SD-card. Other smart features include pre-setting and saving of sonication parameters, LAN connection, and browser remote control.
The table below gives you an indication of the approximate processing capacity of our lab-size sonicators for tissue homogenization and other sample preparation tasks:
| Tavsiya etilgan qurilmalar | To'plam hajmi | Oqim darajasi |
|---|---|---|
| UIP400MTP 96-quduqli plitali sonikator | ko'p quduqli / mikrotitrli plitalar | na |
| Ultrasonik kubok | Flakonlar yoki stakan uchun CupHorn | na |
| GDmini2 | ultratovushli mikro-oqimli reaktor | na |
| VialTweeter | 0,5 dan 1,5 ml gacha | na |
| UP100H | 1 dan 500 ml gacha | 10 dan 200 ml / min |
| UP200Ht, UP200St | 10 dan 1000 ml gacha | 20-200 ml/min |
| UP400St | 10 dan 2000 ml gacha | 20 dan 400 ml / min |
| Ultrasonik elak silkitgich | na | na |
ultratovush apparati UP200Ht kichik namunalarni sonikatsiya qilish uchun 2 mm mikrotipli S26d2 bilan