How To Use Hielscher Ultrasonic Tissue Homogenizers

Before purifying or characterizing intracellular macromolecules such as proteins, organelles, enzymes, or active compounds, it is essential to employ an efficient method for tissue lysis and cell disintegration. Ultrasonication is a highly effective technique for cell preparation, quickly releasing intracellular materials into a buffer solution. The mechanical shear forces generated during ultrasonic tissue homogenization can be precisely adjusted to suit specific materials. This allows for gentle preparation of soft tissues or DNA/RNA shearing with milder sonication, as well as intense ultrasonic cavitation for destructive cell disruption (e.g., for nannochloropsis, yeast).

Below is a selection of tissues, cells, and other biological matter with recommended sonication protocols and guidelines for efficiently preparing your samples using an ultrasonic tissue homogenizer or cell crusher.

Lab ultrasonicator UP200Ht (200W, 26kHz) for sample preparation, homogenization, cell lysis, solubilization of cell suspensions, protein extraction, DNA fragmentation, emulsification, dispersion and mixing applications.

Lab ultra-sonicator UP200Ht (200W, 26kHz) for sample preparation tasks

How to Prepare your Lysates, Homogenates and Extracts from Biological Materials

In alphabetical order:

Acetobacter suboxydans

יישום אולטרא סאונד:
Cell disruption in 5-15 sec.
המלצת Device:
UP200St

Actinomyces

יישום אולטרא סאונד:
Disruption and protein extraction in 3-5 min.
המלצת Device:
UP50H

ALP and LDH activity

יישום אולטרא סאונד:
Determination of ALP and LDH activity and protein content
Frozen cell samples were thawed for 20 min on ice, followed by cell lysis with PBS containing 1% Triton X-100 for 50 min on ice. During cell lysis each sample was sonicated for 1 min at 80 W with an ultrasonic processor UP100H (Hielscher Ultrasonics).
המלצת Device:
UP100H
עיון / מחקר נייר:
Bernhardt, A. et al. (2008): Mineralised collagen—an artificial, extracellular bone matrix—improves osteogenic differentiation of bone marrow stromal cells.

Amorphous tricalcium phosphate (ATCP)

יישום אולטרא סאונד:
ATCP nano-particles, which are an exceptionally reactive precursor for the formation of hydroxyapatite, were dispersed in chloroform containing 5 % (w/w) Tween20 referred to PLGA using the Hielscher ultrasonic device UP400S at 320W for 5 min. applying pulsed intervals (50%) to allow for relaxation of the particles.
המלצת Device:
UP400S
עיון / מחקר נייר:
Mohn, Dirk; Ege, Duygu; Feldman, Kirifl; Schneider, Oliver D.; Imfeld, Thomas; Boccaccini, Aldo R. (2014): Spherical calcium phosphate nanoparticle fillers allow polymer processing of bone fixation devices with high bioactivity. The Free Library 01 May 2010. 21 January 2014.

Anthocyanins

יישום אולטרא סאונד:
Anthocyanins: di-glucosides, mono-glucoside, acylated monoglucosides and acylated di-glucosides of peonidin, malvidin, cyanidin, petunidin and delphinidin:Extraction from grape skin in 40 sec.; pH 5.0; ratio of material/extraction solvent of 1:6.
המלצת Device:
UP100H

Anthraquinones

יישום אולטרא סאונד:
Extraction of Anthraquinones from the roots of Morinda citrifolia
המלצת Device:
UP400S

Apricot seeds

יישום אולטרא סאונד:
Ultrasonic pre-treatment before extracting oil from the seeds of Jatropha curcas L. to improve extraction.
המלצת Device:
UP400S

Artemisia selengensis Turcz

יישום אולטרא סאונד:
Extraction of Rutin (1.0g milled sample in 30 mL methanol) at 25°C in 5-10 min.
המלצת Device:
UP50H

Aspergillus flavus

יישום אולטרא סאונד:
Ultrasonic inactivation of Aspergillus flavus in Sabouraud growth medium
המלצת Device:
UP200S

B. sphaericus / Bacillus sphaericus

יישום אולטרא סאונד:
Disruption in 1-3 min.
המלצת Device:
UP200S

Bacillus anthracis sterne 34F2 spores

יישום אולטרא סאונד:
Ultrasonic removal of Bacillus anthracis sterne 34F2, Bacillus cereus ATCC 21281, and Bacillus thuringiensis ATCC 33680 spores. The use of 150 ppm of sodium hypochlorite along with ultrasound did lead to spore inactivation.
המלצת Device:
UP200S at 100% amplitude
עיון / מחקר נייר:
Pamarthi, S.R. et al.: Effectiveness of Ultrasound in Desoiling Bacillus spp spores Embedded in Complex Food Matrices Attached to Various Contact Surfaces.

Bacillus cereus ATCC 21281 spores

יישום אולטרא סאונד:
Ultrasonic removal of Bacillus anthracis sterne 34F2, Bacillus cereus ATCC 21281, and Bacillus thuringiensis ATCC 33680 spores. The use of 150 ppm of sodium hypochlorite along with ultrasound did lead to spore inactivation.
המלצת Device:
UP200S at 100% amplitude
עיון / מחקר נייר:
Pamarthi, S.R. et al.: Effectiveness of Ultrasound in Desoiling Bacillus spp spores Embedded in Complex Food Matrices Attached to Various Contact Surfaces.

Bacillus subtilis

יישום אולטרא סאונד:
High power, low frequency ultrasound in low volumes of bacterial suspension results in a continuous reduction in bacterial cell numbers i.e. the kill rate predominates. In larger volumes, sonication results in an initial rise in cell numbers suggesting declumping of the bacteria but this initial rise then falls as the declumping finishes and the kill rate becomes more important.
המלצת Device:
UP200St
עיון / מחקר נייר:
Joyce, E.; Phull, S. S.; Lorimer, J. P.; Mason, T. J. (2003): The development and evaluation of ultrasound for the treatment of bacterial suspensions. A study of frequency, power and sonication time on cultured Bacillus species. Ultrason. Sonochem. 10/2003. pp. 315-318.

Barley's Alpha-amylase

יישום אולטרא סאונד:
Stimulating or inhibiting the activity of Barley’s Alpha-amylase: The sample (10 g barley seeds) was dispersed in 80 ml of tap water at direct sonication at ultrasonic intensity of 20, 60 and 100% amplitude, cyle of 50% and with additional agitation. The sonotrode was immersed about 9 mm into the solution. The solution was processed at constant temperature of 30C° for 5, 10 and 15 min.
המלצת Device:
UP200S, amplitudes: 20, 60 and 100%; cyle of 50%; Sonotrode S3, 30C°.
עיון / מחקר נייר:
Yaldagard et al. (2008): Effect of Ultrasonic Power on the Activity of Barley’s Alpha-amylase from Post-sowing Treat of Seeds

Barnacle nauplii

יישום אולטרא סאונד:
Cell disruption/ killing of mesozooplankton: by sonication under following conditions of four flow rates (200, 400, 520 and 800 lh-1) and four amplitudes (25, 50, 75 and 100 %) a killing rate between 61 and 97 % has been achieved.
המלצת Device:
UIP2000hd
עיון / מחקר נייר:
Viitaslo et al. (2005): Ozone, Ultraviolet Light, Ultrasound and Hydrogen Peroxide As Ballast Water TreatmentsExperiments with Mesozooplankton In Low Saline- Brackish Water.

Bifidobacteria strains: B. breve ATCC 15700, B. animalis subsp. Lactis (BB-12), B. longum (BB-46)

יישום אולטרא סאונד:
Bacteria cell destruction and release of ß-galactosidase from Bifidobacteria strains: B. breve ATCC 15700, B. animalis subsp. Lactis (BB-12), B. longum (BB-46): 100 mL pasteurized milk blended with bacteria culture was applied with ultrasound. Cavitation causes the destruction of the bacterial cells and at the same time, release of ß-galactosidase.
המלצת Device:
UIP1000hd: amplitude: 10%, power: 80W, amplitude: 100%, power: 200W; Sonotrode BS2d34; 15-30 min.
עיון / מחקר נייר:
Hung et al. (2009): Ultrasound Aided Yogurt Fermentation with Probiotics.

BL21(DE3) pAtHNL cells (Arabidopsis thaliana cells)

יישום אולטרא סאונד:
Cell disruption: 15 g BL21-(DE3)_pAtHNL cells were slowly suspended in 50 mM potassium phosphate buffer (pH 7.5) at 0 degC.
המלצת Device:
UP200S + sonotrode S14D: at 70W/cm2 (4 x 5 min), cooled in an ice bath
עיון / מחקר נייר:
Okrob, D. et al (2009): Hydroxynitrile Lyase from Arabidopsis thaliana: Identification of Reaction Parameters for Enantiopure Cyanohydrin Synthesis by Pure and Immobilized Catalyst. Adv. Synth. Catal. 2011, 353, 2399 – 2408.

Blood cells (red and white)

יישום אולטרא סאונד:
Disruption in 3-10 sec.
המלצת Device:
UP100H

Boldine from Boldo leaves (Peumus boldus מולינה)

יישום אולטרא סאונד:
תרכובת פעילה חשוב בולדו הוא boldine ((S) -2,9-dihydroxy-1,10-dimethoxiaporphine), המהווה מלבד catechin ((2S, 3R) -2- (3,4-dihydroxy-פניל) -3 ,-1-dihydro 4 (2H) -benzopyran-3,5,7-triol) המרכיבים העיקריים של אלקלואיד שברים פלבנואידים בעלים בולדו. Boldine הוא סוכן חמצון חזק שעובר ניזקים שגורמים הרדיקלים חופשיים בתיווך peroxidative ופועל כמו נבלות רדיקליות הידרוקסיל יעילות.
הפקת נוהל: עבור הליך החילוץ טיפוסי, דגימות עלים בולדו חולצו עם 1 ליטר של מים distillated בלחץ אטמוספרי באמצעות ultrasonicator UIP1000hd ב אצווה מצב הזרימה דרך. זמן של טווחי החילוץ בין 10 ו 40 דק '., בעוצמה אולטרסאונד של 10 עד 23 W / ס"מ2, ומגוון טמפרטורה של C ° 10 כדי 70. התוצאות הכי wer מושגות בתנאים הבאים: עוצמת קולית של סנטימטר / 23 W2 עבור 40 דקות. בטמפרטורה של 36 ° C
תוצאות: תוצאות הניתוח מראות כי sonication ההספק גבוה משפר את שחרורו אנליטי של חומר מטריקס צמחי של בולדו בשיעורים טובים יותר באופן משמעותי לעומת שיטה מקובלת: תשואה שווה שוחררה על ידי sonication ב 30 דקות. תוך זמן החילוץ קונבנציונלי היה 2h.
Chemat (2013) ועמיתים לעבודה הראו כי החילוץ בסיוע באולטרסאונד משפר את היעילות של מיצוי הצמח תוך צמצום זמן השאיבה בריכוז מוגבר של תמציות (אותה כמות של חומר ממיס ו צמח). הניתוח גילה כי התנאים המותאמים היו: sonication כוח 23 W / סנטימטר2 עם UIP1000hd עבור 40 דקות. ובטמפרטורה של 36 מעלות צלזיוס. הפרמטרים אופטימיזציה של מיצוי אולטרסאונד לספק מיצוי טוב יותר לעומת שריה קונבנציונלי מבחינת זמן התהליך (30 דק '. במקום 120 דק'.), תשואה גבוהה, יעילות אנרגיה גבוהה, ניקיון משופרת, בטיחות גבוהה ואיכות מוצר טוב יותר.
המלצת Device:
UIP1000hd עם sonotrode BS2d34 ואת התא זרימה
עיון / מחקר נייר:
Petigny, L.; Périno-Issartier, S.; Wajsman, J.; Chemat, F. (2013): Batch and Continuous Ultrasound Assisted Extraction of Boldo Leaves (Peumus boldus Mol.). היומן הבינלאומי של המדע המולקולרי 14, 2013. 5750-5764.

Bovine serum albumin (BSA)

יישום אולטרא סאונד:
Microencapsulation in poly(lactic-co-glycolic acid) in 40 sec.
המלצת Device:
Dmini
עיון / מחקר נייר:
Freitas et al. (2005): Flow-through ultrasonic emulsification combined with static micromixing for aseptic production of microspheres by solvent extraction.

Brain stem + adrenal gland

יישום אולטרא סאונד:
Dispersion and nucleotid analysis; sample size: 10 mg samples in 10 ml fluid.
המלצת Device:
UP50H

Candida albicans

יישום אולטרא סאונד:
Disruption of 15mL in 9min.
המלצת Device:
UP100H

Carbohydrates, polysaccharides and other functional compounds

יישום אולטרא סאונד:
Extraction of carbohydrates, polysaccharides and other functional compounds
המלצת Device:
UP200St

Carnosic acid from rosemary

יישום אולטרא סאונד:
Extraction of carnosic acid, an active compound, from rosemary.
המלצת Device:
UP400S

Capsaicinoids

יישום אולטרא סאונד:
הפקת capsaicinoids (קפסאיצין, nordihydrocapsaicin) מפלפל צ'ילי: Capsaicinoids מ frutescens פלפלת peppers was obtained via ultrasonic extraction under following conditions: solvent: 95% (v/v) ethanol, solvent/ mass ratio of 10 ml/g, 40 min. sonication extraction time, 45°C extraction temperature. Extractant yield: 85% of the capsaicinoids
המלצת Device:
UP400S

Carotenoids, Beta-Carotenoids

יישום אולטרא סאונד:
Extraction from carrots.
המלצת Device:
UP50H

cDNA

יישום אולטרא סאונד:
Poly-A RNA was purified with the Dynabeads mRNA purification kit (Invitrogen) following the manufacturer’s instructions and was treated for 30 min at 37°C with TURBO DNase (Ambion; 0.2 units/1 μg of RNA). First- and second-strand synthesis followed the manufacturer’s protocol. About 500ng of double-stranded cDNA was fragmented by sonication with Hielscher’s UTR200. DNA was parceled in 2% high-resolution agarose gel and 230–270 bp fragments were cut.
המלצת Device:
UTR200 אוֹ TD_CupHorn
עיון / מחקר נייר:
Delft, J. van; Gaj, St.; Lienhard,M.; Albrecht, M. W.; Kirpiy, A.; Brauers, K.; Claessen, S.; Lizarraga, D.; Lehrach, H.; Herwig, R.; Kleinjans, J. (2012): RNA-Seq Provides New Insights in the Transcriptome Responses Induced by the Carcinogen Benzo[a]pyrene. Toxicological Sciences 130/2, 2012. 427–439.

Caryophanon latum

יישום אולטרא סאונד:
Extraction of glucosamine, muramic acid, alanine, glutamic acid and lysine from caryophanon datum.
המלצת Device:
UP100H

Cellulose

יישום אולטרא סאונד:
Homogenization/ preparation of isotopically homogeneous cellulose.
המלצת Device:
UP200S; in an ice bath
עיון / מחקר נייר:
Laumer et al. (2009): A novel approach for the homogenization of cellulose to use microamounts for stable isotope analyses.

Cellulose nanocrystals (CNC) prepared from eucalyptus cellulose CNCs

יישום אולטרא סאונד:
Cellulose nanocrystals (CNC) prepared from eucalyptus cellulose CNCs were modified by the reaction with methyl adipoyl chloride, CNCm, or with a mixture of acetic and sulfuric acid, CNCa. Therefore, freeze-dried CNCs, CNCm and CNCa were redispersed in pure solvents (EA, THF or DMF) at 0.1 wt%, bymagnetic stirring overnight at (24 ± 1) C, followed by 20 min in a sonication bath using UP100H Hielscher Ultrasonics (Germany), equipped with a 130 W/cm2 sonotrode, at 24 ± 1 degC. After that, CAB was added to the CNC dispersion, so that the final polymer concentration was 0.9 wt%.
המלצת Device:
UP100H; at 24 degC for 20 min.
עיון / מחקר נייר:
Blachechen, L. S. et al (2013): Interplay of colloidal stability of cellulose nanocrystals and their dispersibility in cellulose acetate butyrate matrix. Cellulose 2013.

Cellulose from sugarcane bagasse

יישום אולטרא סאונד:
Extraction of cellulose from sugarcane bagasse
המלצת Device:
UP200St

ChIP assay

יישום אולטרא סאונד:
Ultrasonication is used for cell lysis to release the chromatin. Mild (pulsed) sonication is used to fragment the chromatin. Furthermore, ultrasound accelerates the rate of antibody binding to target proteins and reduces thereby the immunoprecipitation time.
המלצת Device:
UP100H
עיון / מחקר נייר:
Basselet, P. et al. (2008): Sample processing for DNA chip array-based analysis of enterohemorrhagic Escherichia coli (EHEC).
Lauri, A. (2005): Molecular analysis of petal development by X-ChIP and two-hybrid technology. Dissertation University of Cologne 2005.

Chromatin

יישום אולטרא סאונד:
Shearing of chromatin
המלצת Device:
UP400S; at 30% amplitude and 0.5 cycle; on ice.
עיון / מחקר נייר:
Oh et al. (2003): Acetyl-CoA Carboxylase Gene Is Regulated by Sterol Regulatory Element-binding Protein-1 in Liver.

Chromatin extraction

יישום אולטרא סאונד:
The lysate of MEL DS19 cells was sonicated with 10 rounds of 20 s each at 70% of maximum output using the Hielscher 200W ultrasonic processor UP200H
המלצת Device:
Uf200 ः; 70% of maximum output; pulse mode: 10 cycles of 20 sec. each.
עיון / מחקר נייר:
Kang, H. Ch. et al (2010): PIAS1 regulates CP2c localization and active promoter complex formation in erythroid cell-specific a-globin expression. Nucleic Acids Res. 38/ 16, 2010. pp. 5456–5471.

Chromatography

יישום אולטרא סאונד:
Sonication of the adsorbant in the solvent eliminates agglomerates within a few seconds and prepares a uniform, easily packed column. Relevant for adsorbent preparation (e.g. silica gel) prior to column chromatography.
המלצת Device:
UP400S

Cladocerans

יישום אולטרא סאונד:
Cell disruption of mesozooplankton
המלצת Device:
UP2000hd with flow cell
עיון / מחקר נייר:
Viitaslo et al. (2005): Ozone, Ultraviolet Light, Ultrasound and Hydrogen Peroxide As Ballast Water TreatmentsExperiments with Mesozooplankton In Low Saline- Brackish Water.

Copepod adults and copepodites

יישום אולטרא סאונד:
Cell disruption of mesozooplankton: by sonication under following conditions of four flow rates (200, 400, 520 and 800 lh-1) and four amplitudes (25, 50, 75 and 100%) a killing rate between 87 and 99% has been achieved.
המלצת Device:
UIP2000hd with flow cell
עיון / מחקר נייר:
Viitaslo et al. (2005): Ozone, Ultraviolet Light, Ultrasound and Hydrogen Peroxide As Ballast Water TreatmentsExperiments with Mesozooplankton In Low Saline- Brackish Water.

Copepod nauplii

יישום אולטרא סאונד:
Cell disruption of mesozooplankton: by sonication under following conditions of four flow rates (200, 400, 520 and 800 lh-1) and four amplitudes (25, 50, 75 and 100%) a killing rate between 87 and 99% has been achieved.
המלצת Device:
UIP2000hd with flow cell
עיון / מחקר נייר:
Viitaslo et al. (2005): Ozone, Ultraviolet Light, Ultrasound and Hydrogen Peroxide As Ballast Water TreatmentsExperiments with Mesozooplankton In Low Saline- Brackish Water.

COS7-cells

יישום אולטרא סאונד:
Lysis in 400 μl buffer
המלצת Device:
UP200S; 7 cycles
עיון / מחקר נייר:
Zaim (2005): Analyse von Nesprin-2 Defizienten Mäusen.

Cryptosporidium parvaum

יישום אולטרא סאונד:
Ultrasonic inactivation of Cryptosporidium parvaum (protozoa) in water.
המלצת Device:
UP400S
עיון / מחקר נייר:
Tsukamoto, I.; Yim, B.; Stavarache, C. E.; Furuta, M.; Hashiba, K.; Maeda, Y. (2004): Inactivation of Saccharomyces cerevisiae by ultrasonic irradiation. Ultrasonics Sonochemistry 11/2004. pp. 61–65.

Cubosomes

יישום אולטרא סאונד:
Cubosomeseither empty or doped with fluorophore moleculeswere prepared by dispersing the appropriate amount of monoolein in a solution of Pluronic F108 using the ultrasonicator UP100H. To obtain fluorescent cubosomes, the fluorophore was dispersed in the melted monoolein by gentle sonification before dispersion in Pluronic F108. The same procedure was followed when the fluorescent cubosomes were also loaded with quercetin.
Sample: sample size: 4 mLapprox. 96.4 wt% of water, 3.3 wt % of monoolein, 0.3 wt% of Pluronic F108. The percentage of the fluorophore was 2.5 × 10−3 and 2.8 × 10−3 wt%. Amount of added quercetin: 6.4 × 10−6 wt%.
Sonication: UP100H, amplitude 90%, pulse cycle 0.9, for 10 min.
המלצת Device:
UP100H
עיון / מחקר נייר:
Murgia, S.; Bonacchi,S.; Falchi, A. M.; Lampis, S.; Lippolis, V.; Meli, V.; Monduzzi, M.; Prodi, L.; Schmidt, J.; Talmon, Y.; Caltagirone, C. (2013): Drug-Loaded Fluorescent Cubosomes: Versatile Nanoparticles for Potential Theranostic Applications. Langmuir 29, 2013. 6673-6679.

Dekkera bruxellensis

יישום אולטרא סאונד:
Ultrasonic inactivation of Dekkera bruxellensis in water
המלצת Device:
UIP1500hd
עיון / מחקר נייר:
Borthwick, K. A. J.; Coakley, W. T.; McDonnell, M. B.; Nowotny, H.; Benes, E.; Grfschl, .M (2005): Development of a novel compact sonicator for cell disruption. J. Microbio. Meths. 60/2005. pp. 207–216. / Lörincz, A. (2004): Ultrasonic cellular disruption of yeast in water-based suspensions. Biosys. Eng. 89/ 2004. pp. 297–308. / Tsukamoto, I.; Yim, B.; Stavarache, C. E.; Furuta, M.; Hashiba, K.; Maeda, Y. (2004): Inactivation of Saccharomyces cerevisiae by ultrasonic irradiation. Ultrason. Sonochem. 11/2004. pp. 61–65.

Dekkera/ Brettanomyces bruxellensis

יישום אולטרא סאונד:
Inactivation in 90-120 sec.
המלצת Device:
UIP1500hd
עיון / מחקר נייר:
see above

דנ"א

יישום אולטרא סאונד:
DNA fragmentation: 2 min. sonication of 100μL with UP100H or 4 min. sonication of 100μL with UTR200.
המלצת Device:
UP100H, UTR200 אוֹ VialTweeter
עיון / מחקר נייר:
Larguinho M. et al. (2010): Development of a fast and efficient ultrasonic-based strategy for DNA fragmentation.

Drosophila melanogaster S2 cells

יישום אולטרא סאונד:
Extraction of cellular proteins from Drosophila melanogaster S2 cells: Frozen S2 cells (1.56108) were lysed in 1 mL ice-cold homogenization buffer (100 mM Tris-HCl pH 7.5, 1% SDS) and left on ice for 10 min. Cell lysis was completed by ultrasonication on ice (6615 bursts, 0.5 s pulse; 75% intensity) with a Hielscher UP200S ultrasonic processor.
המלצת Device:
UP200S (200W), 75% intensitypulse mode: 6615 bursts, 0.5 s pulse.
עיון / מחקר נייר:
Schwientek, T. et al. (2007): A serial lectin approach to the mucin-type O-glycoproteome of Drosophila melanogaster S2 cells. Proteomics 7, 2007. pp. 3264-3277.

E. coli derivates

יישום אולטרא סאונד:
Cell disruption of E. coli derivates: The frozen pellets corresponding to the sample volume of Vculture = 4/OD mL were resuspended in 580 μL 10 mM potassium phosphate buffer pH 7, 1 mM EDTA. 20 μL of lysozyme (concentration of 1 g L-1) was added and the cells suspension was incubated on ice for about 30 min. Afterwards cells were disrupted by continuous ultrasonication with an ultrasonicator (UP 200S Ultraschallprozessor, Dr. Hielscher GmbH, Teltow) on ice, at an amplitude of 50 % for 20 sec. The soluble and insoluble cell fractions were separated by centrifugation at 13000 rpm for 20 min. The insoluble proteins were washed twice with the 10 mM potassium phosphate buffer pH 7, 1 mM EDTA and stored at –20°C.
המלצת Device:
UP200S with 50% amplitude
עיון / מחקר נייר:
HA (2005): Optimisation of active recombinant protein production, exploring the impact of small heat-shock proteins of Escherichia coli, IbpA and IbpB, on in vivo reactivation of inclusion bodies.

Echinococcus granulosus Antigen

יישום אולטרא סאונד:
Cell lysis and disintegration: The homogenized sample of protein soluble of mature E. granulosus, prepared by freeze-thawing in liquid nitrogen and 42◦C, has been sonicated at 110V, 170W for 3×15 sec on ice. After that, the sample was centrifugated for 15min at 10000g. Protein concentaration was measured by Bradford method and stored at -20◦C.
המלצת Device:
UP200S; 170W, cooling on ice; 3 x 15 sec.
עיון / מחקר נייר:
Tabar et al. (2010): Serodiagnosis of Sheep Hydatidosis with Hydatid Fluid, Protoscolex and Whole Body of Echinococcus granulosus Antigen.

Escherchia coli Gr-

יישום אולטרא סאונד:
Ultrasonic inactivation of Escherchia coli Gr- in milk and juices.
המלצת Device:
UP100H
עיון / מחקר נייר:
Zenker, M.; Heinz, V.; Knorr, D. (2003): Application of ultrasound assisted thermal processing for preservation and quality retention of liquid foods. J Food Prot 66/2003. pp. 1642–1649.

Escherchia coli Gr- in saline

יישום אולטרא סאונד:
Ultrasonic inactivation of Escherchia coli Gr- in saline.
המלצת Device:
UP100H
עיון / מחקר נייר:
Duckhouse, H.; Mason, T. J.; Phull, S. S.; Lorimer, J. P. (2004): The effect of sonication on microbial disinfection using hypochlorite. Ultrason. Sonochem. 11/ 2004. pp. 173–176.

Escherchia coli Gr- in water

יישום אולטרא סאונד:
Ultrasonic inactivation of Escherchia coli Gr- in water.
המלצת Device:
UP100H
עיון / מחקר נייר:
Furuta, M.; Yamaguchi, M.; Tsukamoto, T.; Yim, B.; Stavarache, C. E.; Hasiba, K.; Maeda, Y. (2004): Inactivation of Escherichia coli by ultrasonic irradiation. Ultrason. Sonochem. 11/2004. pp. 57–60.

Glaucoma, optical nerve head

יישום אולטרא סאונד:
RNA fragmentation of optical nerve heads (from rat eyes): The optical nerve head (ONH) was frozen on dry ice and stored at 80°C before extraction. RNA was isolated by sonicating the frozen nerve heads in the kit extraction buffer using an MS 0.5 probe (UP50H) and then, after the instructions provided by the Arcturus kit, including DNase treatment to remove any DNA. Purified RNA was quantified.
המלצת Device:
UP50H with probe MS0.5
עיון / מחקר נייר:
Johnson et al. (2007): Global Changes in Optic Nerve Head Gene Expression after Exposure to Elevated Intraocular Pressure in a Rat Glaucoma Model.

Glycosaminoglycan chondroitin sulfate (CS)

יישום אולטרא סאונד:
Determining CS by Dimethylmethylene Blue Assay
Resuspension of cells: CS pellets in microcentrifuge tubes were resuspended in 0.5 ml of a 0.1mg/ml papain solution in Hank’s balanced salt solution (HBSS) using pulses from an ultrasound horn (UP 100H, Hielscher Ultrasonics GmbH, Germany) at cycle 1 and 100 % amplitude for 3 sec. Thereafter, digestion took place at 60 °C for 24 h.
For Enzyme-linked immunosorbent assay (ELISA), the cells were then suspended in distilled and deionized water at a concentration of 106 cells/ml, and were kept in a freezer at –70°C overnight in order to facilitate cell lysis. The cells were then homogenized by ultrasonication for 40 sec. using the Hielscher ultrasonic tissue homogenizer UP100H.
המלצת Device:
UP100H
עיון / מחקר נייר:
Vandrovcova et al. (2011): Influence of Collagen and Chondroitin Sulfate (CS) Coatings on Poly-(Lactide-co-Glycolide) (PLGA) on MG 63 Osteoblast-Like Cells. Physiol. Res. 60; 2011. 797-813.

HaCaT cells

יישום אולטרא סאונד:
Lysis of HaCaT cells for Chromatin Immunoprecipitation (ChIP): HaCaT cells were fixed with 1% formaldehyde for 10 minutes at 37uC. The fixed cells were lysed in the RIPA buffer and sonicated on ice with an 100W ultrasonic device at amplitude = 1 and duty cycle = 100% in 12 one-minute (12 x 1 min.) pulses.
המלצת Device:
UP100H; for 12 min. on ice,
עיון / מחקר נייר:
Zhang et al. (2007): Basonuclin Regulates a Subset of Ribosomal RNA Genes in HaCaT Cells.

Heparin: Depolymerization of heparin

יישום אולטרא סאונד:
The ultrasonic method enables to produce a low molecular weight heparin (LMWH). Therefore, heparin undergoes a hydrogen peroxide-catalyzed radical depolymerization. The reaction is very fast and takes less than 1 hour, whilst the physicochemical depolymerization process relies on mild reaction conditions, requires no harsh or toxic reagents, and provides a product free of chemical artifacts. Thus, the ultrasonic process is well suited for the large scale production of LMWH, but also analogs produced from natural sulfated polysaccharides.
Ultrasonic Procedure:
For the physicochemical depolymerization of unfractionated heparin by ultrasonic-assisted radical depolymerization, heparin was dissolved in water to a final concentration of 25 mg/mL (5 mL). The reaction mixture was sonicated using a probe-type ultrasonicator UP50H. The ultrasonicator was equipped with a probe (micro tip MS3) with a diameter of 3mm, which provided an amplitude of 180μm. The processor generated mechanical longitudinal vibrations with a frequency of 30 kHz that were produced by electrical excitation. The reaction mixture was maintained at 60°C in a Radleys® reactor and ultrasonic waves were applied as a 0.5sec pulse to prevent mixture heating. A zerotime aliquot (250μl) was removed from the solution prior to reaction launching by simultaneous addition of hydrogen peroxide and application of ultrasonic waves. Hydrogen peroxide was added to obtain a final hydrogen peroxide/heparin (w/w) ratio of 0.15 (3.75 mg/mL).
המלצת Device:
UP50H with sonotrode MS3
עיון / מחקר נייר:
Achour, Oussama; Bridiau, Nicolas; Godhbani, Azza; Le Joubioux, Florian; Bordenave Juchereau, Stephanie; Sannier, Fredéric; Piot, Jean-Marie; Fruitier Arnaudin, Ingrid; Maugard, Thierry (2013): Ultrasonic-assisted preparation of a low molecular weight heparin (LMWH) with anticoagulant activity. Carbohydrate Polymers 97; 2013. 684–689.

Hops

יישום אולטרא סאונד:
Extraction of active compounds / herbal extracts in water and ethanol.
המלצת Device:
UP400S

Lactobacillus (lysis/ DNA isolation of various Lactobacillus strains)

יישום אולטרא סאונד:
Lactobacillus strains: L. pontis, L. sanfranciscensis, L. farciminis, L. panis, L. oris, L. vaginalis and L. reuteri, L. sp.
Procedure: For the DNA isolation of single colonies, an ultrasonic lysis protocol was developed. One colony (2- to 3-mm diameter) was suspended in 100μl lysis buffer (20 mM EDTA, 10 mM Tris [pH 7.9], 1% Triton X-100, 500 mM guanidine-HCl, 250 mM NaCl). Cells were lysed by 1 min of ultrasonication with an probe-type ultrasonicator UP50H. After the addition of 150μl of cold ethanol (-20°C), the mixture was centrifuged over a spin column of the QIAamp tissue kit and finally eluted with 60μl of buffer (10 mM Tris [pH 7,5]).
To have a tool for a fast and reliable identification of single pure cultures, the PCR assay was combined with a fast DNA isolation procedure. Time-consuming enzymatic lysis procedures and variable susceptibility of bacteria to the lysozyme were overcome by ultrasonic treatment of the cells, with subsequent purification and concentration by binding DNA to a silica matrix. The cell material of a single colony was found to be sufficient for the PCR.
המלצת Device:
UP50H
עיון / מחקר נייר:
Müller, M. R. A. (2000): Characterization of the Microbial Ecosystem of Cereal Fermentations using Molecular Biological Methods. Dissertation University of Cologne, 2000.

Lactobacillus acidophilus Gr+

יישום אולטרא סאונד:
Ultrasonic inactivation of Lactobacillus acidophilus Gr+ in milk and juices.
המלצת Device:
UIP500hd
עיון / מחקר נייר:
Zenker, M.; Heinz, V.; Knorr, D. (2003): Application of ultrasound assisted thermal processing for preservation and quality retention of liquid foods. J Food Prot 66/2003. pp. 1642–1649.

Legionella pneumophila Gr+ in diluted medium

יישום אולטרא סאונד:
Ultrasonic inactivation of Legionella pneumophila Gr+ in diluted medium.
המלצת Device:
UIP500hd
עיון / מחקר נייר:
Dadjour, M. F.; Ogino, C.; Matsumura, S.; Nakamura, S.; Shimizu N. (2006): Disinfection of Legionella pneumophila by ultrasonic treatment with TiO2. Water Res 40/2006. pp.1137–1142.

Leuconostoc mesenteroides

יישום אולטרא סאונד:
Leukocyte lysozme activity in myelocytic leukemia: the cell suspension was sonicated for 15 min. and samples assayed for lysozyme activity. The lysozyme concentration of the leukocytes ug./10 cells were determined.
המלצת Device:
UP50H

Leukocytic isozym activity in myelocytic leukemia

יישום אולטרא סאונד:
Sample preparation: cell suspension was ultrasonically treated and samples assayed for lysozyme activity. The lysozyme concentration of the leukocytes ug/106 was determined.
המלצת Device:
UP200St

Malachite Green

יישום אולטרא סאונד:
Sonophotocatalytic degradation of Malachite Green (a strong bactericide): The photocatalytic degradation alone is considerably faster than sonolytic degradation, efficiency can be improved by coupling the two processes. Malachite green, a strong bactericide, is very ecotoxic to marine bacteria but it is converted to organics that are less or not toxic.
המלצת Device:
UP400S

Mangiferin acylation

יישום אולטרא סאונד:
Mangiferin (1,3,6,7-Tetrahydroxy-2- [3,4,5-trihydroxy-6- (hydroxymethyl) oxan-2-י.ל.] xanthen-9-אחד; הנוסחה: C19ח18O11)is a polyphenol of C-glycosylxanthone structure that can be found in many plant species. Mangiferin shows various pharmacological activities. The regioselective acylation of mangiferin can be very efficiently catalyzed by lipase under ultrasonication. Compared with the conventional methods, the ultrasonically-assisted catalysis excels by the advantages of shorter reaction time and higher yields. The optimum conditions for the ultrasonic mangiferin acylation were found as following:
lipase: PCL, התורם acyl: ויניל אצטט; תגובה ממסה: DMSO, טמפרטורת תגובה: 45 degC, כוח קולי: 200W; יחס המצע: התורם acyl / mangiferin 6/1, טוען האנזים: 6 מ"ג / מ"ל
The regioselective acylation yield was up to 84%.
המלצת Device:
UP200St אוֹ UP200Ht
עיון / מחקר נייר:
cp.: Wang, Z.; Wang, R.; Tian, J.; Zhao, B; Wei, X.F.; Su, Y.L.; Li, C.Y.; Cao, S.G.; Wang, L. (2010): The effect of ultrasound on lipase-catalyzed regioselective acylation of mangiferin in non-aqueous solvents. J. Asian Nat Prod. Res. 12/1, 2010. 56-63.

Molecules extraction

יישום אולטרא סאונד:
Extraction protocol for extraction from gypsum, rheolite, basalt, edfell ash and obsidian glass:
A 1g sample of regolith or crushed rock is subject to liquid extraction under ultrasonic irradiation to extract and solubilise target molecules. Therefore, 3ml MeOH P80 was added to 1g analogue sample in a glass test tube and sonicated for 20 minutes with an ultrasonic probe-type device UP50H set at 40% amplitude. The mixture was allowed to stand for 10 minutes, and the cloudy supernatant that had formed above the sedimented analogue sample was decanted into 1.5 ml centrifuge tubes. To minimize loss of extracted components by adsorption to surfaces of the hydrophobic polymer centrifuge tubes, the tubes were first blocked with 0.5% (w/v) BSA in 100 mM HEPES pH 7.4. The supernatants were clarified by centrifugation at 17000 G for 10 minutes and stored at 2 – 8°C in glass vials until tested in the immunoassay.
המלצת Device:
UP50H
עיון / מחקר נייר:
Rix, C.(2012): Detecting Life on Mars and the Life Marker Chip: Antibody Assays for Detecting Organic Molecules in Liquid Extracts of Martian Samples. Dissertation Cranfield University 2012.

Mouse liver pellets

יישום אולטרא סאונד:
The pellets were washed and sonicated for 5 min with a further 0.5 mL of LB2 and centrifuged at 12,000 g for another 20 min, and the resulting two fractions of supernatant were collected. Finally, the pellets were dissolved with 0.5 mL of buffer containing 40 mM tris base, 5 M urea, 2 M thiourea, 4% CHAPS, 100 mM DTT, 0.5% (v/v) biolyte 3-10 (LB3), and were sonicated and centrifuged at 12,000 g for 20 min.
המלצת Device:
UP200S; for 5min.
עיון / מחקר נייר:
Gazzana et al. (2009): An update on mouse liver proteome.

Mouse liver suspension

יישום אולטרא סאונד:
Sample homogenization to produce cell lysate.
המלצת Device:
UP200S; for 3 x 20 sec.
עיון / מחקר נייר:
Gazzana et al. (2009): An update on mouse liver proteome.

Penicillium digitatum

יישום אולטרא סאונד:
Ultrasonic inactivation of Penicillium digitatum (a plant pathogen) in Sabouraud growth medium.
המלצת Device:
UP200St
עיון / מחקר נייר:
López-Malo, A.; Palou, E.; Jiménez-Fernández, M.; Alzamora, S. M.; Guerrero, S. (2005): Multifactorial fungal inactivation combining thermosonication and antimicrobials. J. Food Eng. 67/ 2005. pp. 87–93.

Phycocyanin from Spirulina platensis (Arthrospira platensis)

יישום אולטרא סאונד:
Extraction of phycocyanin from Spirulina platensis (Arthrospira platensis) cells.
המלצת Device:
UP400S

Plant cells and plant tissue

יישום אולטרא סאונד:
30% packed plant cells (W/V) and distilled water are disrupted by sonication for 1-15 min.; plant tissue disintegration: 1 g dried tissue suspended in alcohol is disintegrated during sonication of about 5 min.
המלצת Device:
UP100H

Platelets

יישום אולטרא סאונד:
Platelet lysate preparation: Disruption in 1-5 min.
המלצת Device:
UP200Ht

Pleurotus tuberregium

יישום אולטרא סאונד:
Extraction of polysaccharides from the edible fungus Pleurotus tuberregium
המלצת Device:
UP400S

Poly(lactic-co-glycolic acid) (PLGA)

יישום אולטרא סאונד:
Preparation of bovine serum albumin (BSA)-loaded poly(lactic-co-glycolic acid) (PLGA)by sonication in 40 sec.
המלצת Device:
Dmini; for 40sec.
עיון / מחקר נייר:
Freitas et al. (2005): Flow-through ultrasonic emulsification combined with static micromixing for aseptic production of microspheres by solvent extraction.

Polyphenols from apple

יישום אולטרא סאונד:
Ultrasonic extraction of polyphenols from apple. Solvent: aqueous. Increased yield by 6%; sonication intensity: 20-75Ws/ml; process temp.: heated to 80 degC.
המלצת Device:
UIP2000hd
עיון / מחקר נייר:
Vilkhu, K.; Manasseh, R.; Mawson, R.; Ashokkumar, M. (2011): Ultrasonic Recovery and Modification of Food ingredients. In: Feng/ Barbosa-Cánovas/ Weiss (2011): Ultrasound Technologies for Food and Bioprocessing. New York: Springer, 2011. pp. 345-368.

Polyphenols from black tea

יישום אולטרא סאונד:
Ultrasonic extraction of polyphenols from black tea. Solvent: aqueous. Increased yield by 6-18%; sonication intensity: 8-10Ws/ml; ambient pressure, process temp.: heated to 90 degC.
המלצת Device:
UIP2000hd
עיון / מחקר נייר:
Vilkhu, K.; Manasseh, R.; Mawson, R.; Ashokkumar, M. (2011): Ultrasonic Recovery and Modification of Food ingredients. In: Feng/ Barbosa-Cánovas/ Weiss (2011): Ultrasound Technologies for Food and Bioprocessing. New York: Springer, 2011. pp. 345-368.

Polyphenols from red grape marc

יישום אולטרא סאונד:
Ultrasonic extraction of polyphenols from red grape marc. Solvent: aqueous. Increased yield by 11-35%; sonication intensity: 20-75Ws/ml; ambient pressure.
המלצת Device:
UIP2000hd
עיון / מחקר נייר:
Vilkhu, K.; Manasseh, R.; Mawson, R.; Ashokkumar, M. (2011): Ultrasonic Recovery and Modification of Food ingredients. In: Feng/ Barbosa-Cánovas/ Weiss (2011): Ultrasound Technologies for Food and Bioprocessing. New York: Springer, 2011. pp. 345-368.

Polyphenols, amino acid and caffeine from green tea

יישום אולטרא סאונד:
Extraction of active compounds from Green Tea in water
המלצת Device:
UP400S

Pork: Brining of pork loins

יישום אולטרא סאונד:
For ultrasonically assisted brining, pork loins (Longissimus dorsi) were immersed in sodium chloride brine (40 g L−1) and treated at 5°C with low-frequency ultrasound (20 kHz) at low-intensity (2–4 W/cm−2). The effect of the ultrasonic assisted curing on porcine tissue microstructure, protein denaturation, water-binding capacity (WBC), water-holding capacity (WHC), sodium chloride diffusion coefficient (D) and on meat textural profile (TPA) was investigated. Results showed that the ultrasonic treatment caused favourable microstructural changes in meat tissue. Water-holding capacity and textural properties were improved by ultrasonic treatment compared to both tumbled and static brined samples. However, these positive effects were highly dependent on ultrasonic intensity. Higher intensities and/or longer treatment times caused denaturation of proteins. The constant diffusion coefficient model was able to describe precisely the NaCl diffusion kinetics during brining. Ultrasonic treatment significantly enhanced the diffusion of salt compared to samples brined under static conditions and diffusion coefficient exponentially increased with increased ultrasonic intensity.
המלצת Device:
UP200Ht with sonotrode S26d40
עיון / מחקר נייר:
Siro, I.; Ven, Cs.; Balla, Cs.; Jonas, G.; Zeke, I.; Friedrich, L. (2009): Application of an ultrasonic assisted curing technique for improving the diffusion of sodium chloride in porcine meat. Journal of Food Engineering 91/2, 2009. 353–362.

Porphyra yezoensis

יישום אולטרא סאונד:
Ultrasonic degradation of polysaccharides from Porphyra yezoensis: 50mL of 1.0 g/100mL porphyra yezoensis polysaccarides (dry weight) solution have been sonicated for 4 hr with an UP400S.
המלצת Device:
UP400S; pulsed ultrasound (cycles: 2 sec on/ 2 sec off) at 20°C.

Powders

יישום אולטרא סאונד:
Grinding, deagglomeration and dispersion to small, relativley uniform particle size.
המלצת Device:
UP200St

Rat bones

יישום אולטרא סאונד:
Disruption of 1 gm in 5-7 min.
המלצת Device:
UP200St

Rat skin

יישום אולטרא סאונד:
Disruption of 1 gm in 1 min.
המלצת Device:
UP400S

Rawolfia Serpentina

יישום אולטרא סאונד:
Extraction of the alkaloid reserpine.
המלצת Device:
UP100H

Rebaudioside A

יישום אולטרא סאונד:
Samples of 10g dry and ground stevia leaves were extracted in 100mL water under continuous stirring (with a magnetic stirrer). The pH value was controlled with 0.01 M pH 7 sodium phosphate. The sample was put in a 150 mL glass beaker and sonicated with an probe-type ultrasonicator (UIP500hd, 20kHz, 500W). The tip of the sonotrode was immersed about 1.5 cm into the slurry of stevia leaves. The ultrasonic device was set for a power output of 350W. A mild sonication treatment of 350 W for 5-10 min. at a constant process temperature of 30°C gave a rebaudioside A yield of 30-34g per 100g sample. After sonication, the extract solution was centrifuged and filtered off through 0.45 μm microporous membrane; the filtrate was taken for total rebaudioside A content analysis. The extraction yield of total rebaudioside A content was analyzed by HPLC.
עד הממס ללא המיצוי ultrasonically בסיוע, תשואה גבוהה של A rebaudioside הושגה לעומת שיטות חילוץ מסורתיות כגון מיצוי או שרייה חומות.
המלצת Device:
UIP500hd

Rice starch

יישום אולטרא סאונד:
Disruption, starch isolation, and break of noncovalent bonds between protein and starch in a rice flour slurry (33%) in 2040 min.
המלצת Device:
UP500hd; 2040 min.

Rotifers

יישום אולטרא סאונד:
Cell disruption of mesozooplankton: by sonication under following conditions of four flow rates (200, 400, 520 and 800lh-1) and four amplitudes (25, 50, 75 and 100%) a killing rate between 58 and 85% has been achieved.
המלצת Device:
UP2000 with flow cell
עיון / מחקר נייר:
Viitaslo et al. (2005): Ozone, ultraviolet light, ultrasound and hydrogen peroxide as ballast water treatmentsExperiments with mesozooplankton in low-saline brackish water. Journal of Marine Environmental Engineering, 8(1), 35-55.

S. fragilis

יישום אולטרא סאונד:
release of galactokinease in 4 min.
המלצת Device:
UP200St

Saccharomyces cerevisiae

יישום אולטרא סאונד:
Ultrasonic inactivation of Saccharomyces cerevisiae in Sabouraud growth medium and in saline.
המלצת Device:
UP400S
עיון / מחקר נייר:
Yap, A.; Jiranek, V.; Grbin, P.; Barnes, M.; Bates, D. (2007): Studies on the application of high power ultrasonics for barrel and plank cleaning and disinfection. Austr. NZ Wine Indust. J. 22(3)/ 2007. pp. 96–104.

Salvia miltiorrhiza bunge

יישום אולטרא סאונד:
Extraction of biological active compounds: sodium Danshensu and four tanshinones (dihydrotanshione I, tanshinone I, cryptotanshinone and tanshinone IIA).
המלצת Device:
UP100H

Salvia Officinalis

יישום אולטרא סאונד:
Extraction of active compounds from Salvia Officinalis (sage) in less than 2hr.
המלצת Device:
UP50H

Serum

יישום אולטרא סאונד:
Homogenization
המלצת Device:
UP200Ht

Sheep cystic livers, lungs and positive blood (Echinococcus granulosus antigens)

יישום אולטרא סאונד:
Sheep cystic livers, lungs and positive blood (Echinococcus granulosus antigens in lambs): The sample was then sonicated for 2 × 15 sec on ice until no intact protoscolices were visible.
המלצת Device:
UP200S; 2 x 15 sec.
עיון / מחקר נייר:
Tabar et al. (2009): Antibody response against hydatid fluid, protoscolex and whole body of Echinococcus granulosus antigens in lambs. Journal of Veterinary Research, Volume 10, Issue 3; 2009. 283-288.

Sorbitant Trioleate, ethanol (as solvent) and Bi-2212 powder

יישום אולטרא סאונד:
Deagglomerate a slurry containing the dispersant Sorbitant Trioleate, ethanol (as solvent) and Bi-2212 powder.
המלצת Device:
UP200S; for 3 min.
עיון / מחקר נייר:
Mora et al. (2009): Fabrication of Superconducting Coatings on Structural Ceramic Tiles.

Soy isoflavones

יישום אולטרא סאונד:
ultrasonic extraction of soy isoflavones in water and solvent. Increased yield of up to 15% in extraction efficiency.
המלצת Device:
UP200St
עיון / מחקר נייר:
Rostagno et al. (2003), referenced by Vilkhu, K.; Manasseh, R.; Mawson, R.; Ashokkumar, M. (2011): Ultrasonic Recovery and Modification of Food ingredients. In: Feng/ Barbosa-Cánovas/ Weiss (2011): Ultrasound Technologies for Food and Bioprocessing. New York: Springer, 2011. pp. 345-368.

Soy protein

יישום אולטרא סאונד:
Ultrasonic extraction of soy protein in water and alkali (sodium hydroxide). Increased yield by 53%. Inline sonication was found more efficient as batch extraction (inline sonication gave a 23% higher yield than batch sonication).
המלצת Device:
UIP1000hd for batch/ beaker and with flow cell reactor for inline sonication.
עיון / מחקר נייר:
Moulton and Wang (1982), referenced by Vilkhu, K.; Manasseh, R.; Mawson, R.; Ashokkumar, M. (2011): Ultrasonic Recovery and Modification of Food ingredients. In: Feng/ Barbosa-Cánovas/ Weiss (2011): Ultrasound Technologies for Food and Bioprocessing. New York: Springer, 2011. pp. 345-368.

Sperm heads (human)

יישום אולטרא סאונד:
Disruption in 10 min.
המלצת Device:
UP100H

Sperm tails (human)

יישום אולטרא סאונד:
Disruption immediately
המלצת Device:
UP100H

Stevia rebaudiana Bert.

יישום אולטרא סאונד:
Ultrasonic extraction of stevioside glycoside from samples of 10 g of dry leaves from Stevia rebaudiana with four particles sizes (0.315 mm, 2 mm, 6.3 mm and crushed dry leaves) were mixed with different solvents: distilled water and water/ethanol mixtures (55% and 70%) with different sample weight to solvent volume ratios: 1/10, 1/8, 1/5 (w/v) then exposed to ultrasound at room temperature. Sonication time: < 5 min. המלצת Device:
UP200St

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מדריך זה מסביר איזה סוג של סוניקטור הוא הטוב ביותר עבור משימות הכנת הדגימה שלך כגון ליזה, שיבוש תאים, בידוד חלבונים, פיצול DNA ו- RNA במעבדות, ניתוח ומחקר. בחר את סוג הסוניקטור האידיאלי עבור היישום שלך, נפח הדגימה, מספר הדגימה והתפוקה. Hielscher Ultrasonics יש הומוגנייזר קולי אידיאלי בשבילך!

כיצד למצוא את הסוניקטור המושלם לשיבוש תאים ומיצוי חלבונים במדע וניתוח

תמונה ממוזערת של וידאו

 

High Performance Ultrasonicators for Any Size

Hielscher Ultrasonics cell disruptors and tissue homogenizers are available at any size for any volume. Whether you have to sonicate small sample sizes, mass samples such 96-well plates, mid-size volumes or truckloads per hour, our portfolio offer the ideal ultrasonicator for your application. Compact, hand-held ultrasonic homogenizers are optimal for lab and research, whilst our industrial series covers everything between 0,5kW to 16kW per ultrasonic processor. With the capability of being installed as clusters, with Hielscher ultrasonicators you can process virtually any volume. The robustness of Hielscher’s ultrasonic equipment allows for 24/7 operation at heavy duty and in demanding environments.
The sophisticated and smart software allows for highest process control and operator’s convenience. All sonication data are automatically recorded on a built-in SD-card. Other smart features include pre-setting and saving of sonication parameters, LAN connection, and browser remote control.

The table below gives you an indication of the approximate processing capacity of our lab-size sonicators for tissue homogenization and other sample preparation tasks:

התקנים מומלציםנפח תצווהקצב זרימה
UIP400MTP 96-Well Plate Sonicatormulti-well / microtiter platesN.A.
cuphorn אולטרסאונדCupHorn for vials or beakerN.A.
GDmini2ultrasonic micro-flow reactorN.A.
VialTweeter0.5 to 1.5mLN.A.
UP100H1 עד 500mL10 עד 200mL / min
UP200Ht, UP200St10 to 1000mL20 to 200mL/min
UP400St10 עד 2000mL20 עד 400mL / min
Ultrasonic Sieve ShakerN.A.N.A.

Ultrasonicator UP200Ht with microtip S26d2 for ultrasonic lysis of biological samples

Ultrasonicator UP200Ht with 2mm microtip S26d2 for the sonication of small samples

We will be glad to discuss your process.

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