Yuqori o'tkazuvchanlik uchun ultratovushli ajitatsiyalangan shprits filtri
Ultrasonically agitated syringe filters enable to work at higher flow rates and loading capacities. Samples with higher solid contents can be reliable and efficiently processed using an ultrasonically vibrated syringe filters. This allows for higher throughputs. Ultrasonically agitation of syringe filters can be applied to a variety of membrane types and pore sizes matching your process requirements.
Syringe Filters – Sonicated for Higher Filtration Efficiency
The membrane of a syringe filter is the crucial part that functions as the actual filter and are available in various sizes (diameter), pore sizes and made from different materials such as polyamide (PA), polytetrafluoroethylene (PTFE), polyvinylidene fluoride (PVDF), cellulose acetate (CA), regenerated cellulose (RC), polypropylene (PP), cellulose mixed ester (CME) and polyether sulfone (PES).
When biological samples are treated, the different intensity of adsorption of proteins by different filter materials should be considered. Many syringe filters are available in both non-sterile and sterile versions. Filtration using a syringe filter is often a slow, time consuming process, which is also known for sample loss due to dead volume and incomplete filtration. Ultrasonic agitation of syringe filters makes the filtration process significantly more efficient – resulting in faster and more complete filtration results.
Ultrasonicator UP200St (200W, 26kHz) with sonotrode S26s26spec for the agitation of syringe filters. The ultrasonic agitation enables for high throughputs in syringe filters.
In laboratories, where microbiological samples or cell cultures are processed and filters, syringe filters are used to remove bacteria from a solution with thermolabile ingredients (e.g. vitamins or antibiotics in the culture medium). Since these samples cannot be steam-sterilized in an autoclave at 121°C, filtration with a syringe filter is the most commonly used technique of separation. For sterile filtration of a volume of up to 100 ml, syringe filters with a pore size of 0.2 µm or 0.45 µm are most commonly used; however, viruses and mycoplasmas are not retained with either pore size. The so-called “Bacteria Challenge Test” is an assessment that provides information on which types of bacteria are retained with a syringe filter of a certain pore size.
Filters with a pore size of 5 µm are used for pre-filtration in order to remove fractions containing larger particles. Pre-filtration is particularly helpful when a high proportion of solids is present in the sample, which would immediately clog a fine filter membrane.
Hielscher Ultrasonics offers a facile and reliable solution to enhance the filtration efficiency of your syringe filter!
- sample preparation before analysis
- HPLC
- UHPLC
- microbiological samples
- cell suspensions, cell cultures
- pre-filtration
Hielscher Ultrasonics’ Solution for Improved Syringe Filtration
Ultrasonically agitated syringe filters are highly efficient in removing particulate impurities from liquid samples.
The sonotrode S26d26spec works with the ultrasonic processor UP200St and is readily available for sale. Maximum power coupling at 100% amplitude approx. 40 Watts to the filter. Cavitation noise is audible, when the filter is filed with water. Attention: Basic laws of thermodynamics apply. All power will finally heat the filter and its content. At up to 40W that creates a measurable increase in temperature. In order to avoid damage of the sample and syringe filter, lower amplitudes and pulse operation (short intense burst followed by several seconds of idle time for heat dissipation) are recommended.
The ultrasonic sonotrode can be easily adapted and customized to specific syringe filter types.
Special sonotrode for the simultaneous agitation of several syringe filters are available, too.
Biz bilan bog'lanish! / Bizdan so'rang!
Ultrasonikator UP200St (200W, 26kHz) consists in an ultrasonic transducer and generator. The UP200St ultrasonic system is used successfully for the agitation of syringe filters resulting in an enhanced filtration efficiency.
Adabiyot / Adabiyotlar
- Larry Scheer (2009): Analytical sample preparation: The use of syringe filters. Filtration & Separation, Volume 46, Issue 1, 2009. 32-33.
- Marilyn E. Holt, Lauren E. Salay, Walter J. Chazin (2017): Chapter Twelve – A Polymerase With Potential: The Fe–S Cluster in Human DNA Primase. In: Sheila S. David (Ed.): Methods in Enzymology, Academic Press, Volume 59, 2017. 361-390.
- Shin, Woo-Jin, Hyung-Seon Shin, Ji-Hun Hwang, and Kwang-Sik Lee (2020): Effects of Filter-Membrane Materials on Concentrations of Trace Elements in Acidic Solutions. Water Vol. 12, 2020. 3497.
Hielscher Ultrasonics kompaniyasi yuqori samarali ultratovushli homogenizatorlarni ishlab chiqaradi laboratoriya uchun sanoat hajmi.