Biologik parchalanadigan nanosferalarni ishlab chiqarish

Biologik parchalanadigan mikro va nanosferalar doimiy, kontaktsiz va ifloslanishsiz jarayonda ishlab chiqarilishi mumkin, ular steril sharoitlarda oson ishlaydi.

Kirish

P

Hielscher kontaktsiz sonikatsiya oqim hujayrasi Dmini yordamida nanzarrachalarni ifloslanishsiz sintez qilish uchun sozlash sxemasi

Ultratovushli kontaktsiz nano-sfera ishlab chiqarish usuli

PLGA50:50 nanoparticles (Resomer® RG503H, Boehringer Ingelheim) were produced using a modified solvent extraction/evaporation process. PLGA dissolved in dichloromethane (2 or 5%) was dispersed in aqueous 0.5% (w/w) PVA-solution by means of the novel experimental set-up involving a contact-free flow-through ultrasonication cell Dmini. The coarse O/W-dispersion was first premixed by a magnetic stirrer and then homogenized in the contact-free ultrasonic flow-through cell Dmini (flow rates of O- and W-phases were at 1:8). The initially formed PLGA-solvent nanodroplets gradually solidified during the passage in the tubes to become PLGA nanoparticles. Final hardening of the particles was achieved in a larger volume of 0.5% PVA solution.

Ultrasonik kontaktsiz oqim hujayrasi Dmini ifloslanishsiz nanopartikullar sintezi uchun

Natijalar

O'rtacha diametri 485 nm bo'lgan nanozarrachalar DCMdagi 2% PLGA eritmasidan 32 Vt sonikatsiya kuchida tayyorlandi (1-tab.). Hajmi taqsimoti engil quyqa bilan mono-modal edi (3A-rasm). Nanozarrachalar o'lchamlari 10 va 90% persentillarga muvofiq 175 dan 755 nm gacha uzaytirildi. Ishlab chiqarish jarayonining takrorlanishi doimiy ravishda yaxshi edi, chunki o'rtacha zarracha diametrining faqat kichik o'zgaruvchanligi aks ettirilgan. Pastga tushirish Jadval 1. Turli sharoitlarda tayyorlangan PLGA50:50 nanospheres ning o‘rtacha diametri. Ikki to‘plam o‘rtachasi ± absolyut farq.[/caption]

Nihoyat, ko'proq hidrofilik PLGA zarrachalarning o'rtacha hajmi va hajmi taqsimotida sezilarli o'zgarishlarsiz ko'proq hidrofobik va past molekulyar og'irlikdagi PLA ga almashtirildi. 2% li polimer eritmalaridan tayyorlangan zarrachalarning turli partiyalarining morfologiyasida farqlar kuzatilmadi. Ularning barchasi mukammal sharsimon shakllar va silliq sirtlarni ko'rsatdi (3B-rasm). 5% PLGA eritmasidan tayyorlangan zarralar esa kamroq sharsimon bo'lib, bir oz ajinlangan yuzalarni va ikki yoki ba'zan undan ko'p zarrachalarning sintezini ko'rsatdi (3C-rasm).

3-rasm: PLGA nanozarrachalari. (A): 2% / 32W, 5% / 32W va 2% / 25W% polimer konsentratsiyasi/sonikatsiya quvvatida tayyorlangan zarrachalarning hajmi taqsimoti; yashash vaqti = 14 s. (B), (C): 2 va 5% polimer eritmalaridan tayyorlangan zarrachalarning SEM rasmlari. Yashash vaqti = 14s; sonikatsiya quvvati = 32 Vt. Barlar 1 mikronni bildiradi.
(O'rganish va rasmlar: © Freitas va boshqalar, 2006)

Muhokama va Xulosa

Ultrasonik oqim hujayrasi biologik parchalanadigan polimer nanospheres ni emulsiyon-eroziya/eruvchan moddalarni ajratish asosida ishlab chiqarishga yaxshi mos kelishi aniqlangan. Kelajak tadqiqotlar jarayonni kattalashtirish va yanada mayda emulsiyalar hosil qilish uchun quvvat kirishini oshirishga qaratiladi. Bundan tashqari, hujayraning suv-moy emulsiyalarini tayyorlash uchun, masalan, keyinchalik dori modda bilan yuklangan mikrosferalarga aylantirish uchun mosligi o‘rganiladi.

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