Ultrazvočno hladno vodo ekstrakcija Psilocybin

Ultrasonic Psilocybin Extraction

Ultrasonic extraction is used successfully in many industries such as food, pharmaceutical, cosmetic, and fine-chemical industry to extract and isolate bioactive compounds and other substances from raw materials. The ultrasonic technique is considered as highly efficient, rapid, reliable, safe and easy to operate as well as environmental-friendly extraction method. Due to these advantages, the ultrasonically-assisted extraction (UAE) outperforms alternative extraction methods. Compounds such as psilocybin, psilocin, baeocystin and norbaeocystin are already successfully extracted using Hielscher Ultrasonics equipment. The ultrasonic extraction of psychedelic compounds gives high yields of superior extract quality within a turbo-extraction procedure. Ultrasonic extraction is compatible with any solvent and allows for the production of full-spectrum extracts.

Ultrazvočna ekstrakcija – A Cold Extraction Technique

Bioactive compounds are heat-sensitive, which means they degrade and decompose under high temperatures so that they loose their effect and potency. Therefore, most botanical extraction benefit from a non-thermal extraction method, which is not based on heat a basic working principle. Ultrasonic extraction is a purely mechanical extraction technique, which working principle is the generation of acoustic cavitation.
What is Ultrasonic Cavitation and How does it Impact Extraction?
For instance, when a liquid is sonicated with an ultrasound probe at 20kHz, 20,000 vibrations per second are transmitted into the medium. The ultrasonic waves travel through the liquid, where they create alternating high-pressure (compression) / low-pressure (rarefaction or expansion) cycles. During the low-pressure vacuum cycle, minute vacuum bubbles or cavities occur in the liquid, which grow over several pressure cycles. During the compression phase of the liquid and bubbles, the pressure is positive, while the rarefaction phase produces a vacuum (negative pressure.) During the compression-expansion cycles, the cavities in the liquid grow until they reach a size, at which they cannot absorb more energy. At this point, they implode violently. The implosion of those cavities results in various highly energetic sonomechanical effects, which are known as the phenomenon of acoustic / ultrasonic cavitation. During the implosion of ultrasonic cavitation bubbles, extreme energy dense forces such as high shear, liquid streaming and turbulences are created in the sonicated liquid. These cavitational forces disrupt cell walls of plant tissues and release the intracellular compounds, including bioactive substances such as the hallucinogenics psilocybin, psilocin, baeocystin and norbaeocystin from mushroom species. The alternating high-pressure/low-pressure cycles also promote intense mass transfer rates, which results in the superior extraction yield obtained by sonication. Ultrasonic extraction can release the complete active substance content from botanicals.

Ultrasonic Psilocybin Extraction: Step-by-Step

You can extract psilocybin and other bioactive substances from mushrooms using water, ethanol or 200 proof ethyl alcohol as solvents.
Chop the fresh or dried psilocybin mushrooms into small bits and pieces. You can use caps and stems. Both contain the psychoactive compounds, however the caps consistently are richer in psilocybin. The spores do not contain psilocybin.
Place the chopped mushrooms in a glass beaker and fill it with cold water.
Optionally, you might place the glass beaker in an ice bath to keep the process temperature as cold as possible.
The sonicated slurry turn blue with blue water containing extracted psilocybin from the mushroom fruits.
Separate the mushroom pieces and debris by straining or filtering. Optionally, keep the mushroom material from filtration for a second extraction run using either water or ethanol.
Use an evaporation system to remove the solvent. 1 to 2 grams of dried magic mushrooms should yield in approx. 1cc of final extract. As mushrooms are a natural product, the content of psychedelic substances can vary.
Fill the final extract into a dark glass container or vial and store in the freezer.
By an optional crystallization step, the shelf-life of the psilocybin extract can be prolonged further.

Psilocybin-Containing Mushrooms

Psilocybin can be found in more than 200 species of mushrooms of the four fungus genera Conocybe. Panaeolus, Psilocybe, Stropharia, which are all suitable for extracting the the active compound psilocybin ultrasonically. In general, mushrooms that contain psilocybin exhibit the following characteristics: They have dark spores, are lamella / gills fungi, grow mostly in humus-rich soil in meadows and woods of tropical and sub-tropical regions and exhibit blue bruises which occurs due to an oxidative reaction, when the mushroom is handled or damaged.
The most well-known species of psilocybin-containing mushroom is Psilocybe cubensis, which contains two psycho-active compounds, psilocybin and psilocin. Commonly known as shrooms, magic mushrooms, golden tops, cubes, or gold caps, Psilocybe cubensis belongs to the fungus family Hymenogastraceae, and was formerly known as Stropharia cubensis. As it is widely distributed and easy to cultivate, Psilocybe cubensis is the best-known pscilocybin-containing mushroom species.
Psilocybe cubensis is probably the most famous for its hallugcinogenic effects, which are caused mainly by the active compounds triggering psychedelic experiences after ingestion. Its major psychoactive compounds are:

• Psilocybin (4-phosphoryloxy-N,N-dimethyltryptamine)
• Psilocin (4-hydroxy-N,N-dimethyltryptamine)
• Baeocistin (4-fosforiloksi-N-metiltiptamin)
• Norbaeocistin (4-fosforiloksitryptamine)

Prednosti ultrazvočnih ekstrakcijskih sistemov

The advantages of ultrasonic extraction are the high extraction yield of psychedelic compounds (i.e. psilocybin, psilocin, baeocystin, norbaeocystin), the free choice of solvent (e.g. water, ethanol, water-alcohol mixture etc.), as well as the simple and safe operation. Due to the intensive mechanical forces of sonication, ecological and mild solvents such as water, ethanol etc. are usually sufficient to achieve an extraordinary extraction rate and yield. As a result, ultrasonic extraction shortens the extraction time and enables a reduced use of solvents or the use of milder, gentler solvents. This means that ultrasonic extraction allows for both, higher extraction rates and healthier extracts (e.g. cold water extracts). Since the process temperature can be precisely controlled during the sonication, thermal decomposition of the extracts due to excessively high temperatures as well as evaporation of the substances are avoided.

Sophisticated Ultrasonicators for Mushroom Extraction

Hielscher Ultrasonics product portfolio covers the full range of high-performance ultrasonic extractors from small to large scale. Additional accessories allow for the easy assembly of the most suitable ultrasonic extractor configuration for your process. The optimal sonication setup depends on the envisaged capacity, volume, raw material, batch or inline process and timeline. Our long-experiences, well-trained staff will recommend you the most suitable ultrasonic extractor and will assist you during the establishing phase of your mushroom extraction process.

Ultrazvočna ekstrakcija – Serija in v vrstici

Hielscher ultrasonicators can be used for batch and continuous flow-through processing. Depending on your process volume and hourly throughput, inline processing might be recommended for higher processing volumes. Whilst batching of large violumes is time- and labour-intensive, a continuous inline mixing process comes handy as it is more efficient, faster and requires significantly less labour. Hielscher Ultrasonics has the most suitable extraction setup for your extraction volume and process goals.

Ultrazvočni ekstraktorji za vsako zmogljivost izdelka

Hielscher Ultrasonics paleto izdelkov zajema celoten spekter ultrazvočnih procesorjev iz kompaktnih lab ultrasonicators nad klopi-top in pilotni sistemi za popolnoma industrijske ultrazvočne procesorje z zmogljivostjo za obdelavo truckloads na uro. Celotna ponudba izdelkov nam omogoča, da vam ponudimo najprimernejši ultrazvočni ekstraktor za vašo surovino, procesno zmogljivost in proizvodne cilje.
Ultrazvočni klopi sistemi so idealni za preskuse izvedljivosti in optimizacijo procesov. Linearna lestvica, ki temelji na uveljavljenih procesnih parametrih, omogoča zelo enostavno povečanje predelovalnih zmogljivosti od manjših sklopov do v celoti komercialne proizvodnje. Up-povečanje je mogoče storiti bodisi z namestitvijo močnejši ultrazvočni ekstraktor enoto ali grozdenje več ultrasonicators vzporedno. Z UIP16000, Hielscher ponuja najmočnejši ultrazvočni ekstraktor po vsem svetu.

Natančno nadzorovati Amplitudes za optimalne rezultate

All Hielscher ultrasonicators are precisely controllable and thereby reliable work horses in production. The amplitude is one of the crucial process parameters that influence the efficiency and effectiveness of ultrasonic extraction of bioactive phyto-nutrients and hallucinogenic compounds from botanicals. Raw material such as soft fresh mushrooms require milder sonication and lower amplitude settings, whilst dried mushrooms might require sonication at higher amplitudes to release the intracellular content. All Hielscher Ultrasonics’ procesorji omogočajo natančno nastavitev amplitude. Sonotrodes in booster rogovi so dodatki, ki omogočajo, da spremenite amplitudo v še širšem območju. Hielscher je industrijski ultrazvočni procesorji lahko poda zelo visoke amplitude in poda potrebno ultrazvočno intenzivnost za zahtevne aplikacije. Amplitude do 200 μm lahko enostavno neprekinjeno izvajamo v 24/7 delovanju.
Natančne nastavitve amplitude in stalno spremljanje ultrazvočnih procesnih parametrov prek pametne programske opreme vam omogočajo zdravljenje semen z najučinkovitejšimi ultrazvočnimi pogoji. Optimalno ultrazvočno razbijanje za najboljše rezultate ekstrakcije!
Robustnost Hielscher je ultrazvočna oprema omogoča 24 / 7 delovanje na težkih in v zahtevnih okoljih. To naredi Ultrazvočna oprema Hielscher je zanesljivo delovno orodje, ki izpolnjuje vaše zahteve ekstrakcije.

Enostavno, brez tveganja Testiranje

Ultrazvočni procesi se lahko popolnoma linearno. To pomeni, da lahko vsak rezultat, ki ste ga dosegli z laboratorijem ali ultrazvočnim programom na klopi, pomanjšate na popolnoma enak izhod z uporabo popolnoma enakih procesnih parametrov. To naredi ultrasonication idealen za testiranje brez tveganja izvedljivosti, optimizacijo procesov in naknadno izvajanje v komercialno proizvodnjo. Pišite nam, da se naučijo, kako lahko ultrazvočno razbijanje poveča vaš ohrovt donos in kakovost.

Najvišja kakovost – Izdelano in izdelano v Nemčiji

Kot družinsko voden in družinsko vodeno podjetje Hielscher daje prednost najvišjim standardom kakovosti za svoje ultrazvočne procesorje. Vsi ultrasonicators so zasnovani, izdelani in temeljito testirajo v naši sedež v Teltow blizu Berlin, Nemčija. Robustnost in zanesljivost Hielscher je ultrazvočne opreme, da je delovni konj v vaši proizvodnji. 24/7 operacija pod polnim tovorom in v zahtevnih okoljih je naravna značilnost Hielscher je visoko zmogljivih mešalniki.

You can buy Hielscher ultrasonic processor in any different size and exactly configured to your process requirements of mushroom extraction. From treating fungi in a small lab beaker to the continuous flow-through mixing of mushroom slurries on industrial level, Hielscher Ultrasonics offers a suitable ultrasonicator for you! Please contact us – veseli smo, da vam priporočamo idealno ultrazvočno nastavitev!
V spodnji tabeli vam daje podatek o približni zmogljivosti obdelave naših ultrasonicators: