Dandelion ExtractionPotent Tinctures with Sonication

Ultrasonic extraction of dandelion plant parts is a highly efficient and effective method to obtain bioactive compounds under mild extraction conditionsyielding a high-quality extracts and tinctures. Learn how to apply ultrasonication to maximize extraction yields while preserving the integrity of the valuable phytochemicals found in dandelion leaves, flowers, and roots.

Dandelion Tinctures produced by Sonication

Ultrasonic extraction of dandelion (Taraxacum officinale) offers numerous advantages over traditional extraction methods, particularly in terms of efficiency, yield, and preservation of bioactive compounds. The process involves using low-frequency, high-intensity ultrasound waves to disrupt plant cells, facilitating the release of intracellular contents into the solvent. Thereby, sonication intensifies and accelerates the preparation of potent dandelion tinctures. For ultrasonic dandelion tinctures, you can use all parts of the dandelion (taraxacum officinale) plant:

  • the roots
  • the leaves
  • the flowers.

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Sonicator UP200Ht for dandelion extraction. Ultrasonics extraction is used to extract bioactive compounds from dandelion flowers, leaves and roots.

Sonicator UP200Ht for dandelion extraction

Step-by-Step Instructions for Ultrasonic Dandelion Extraction

Below, we walk you through the extraction from each dandelion plant part separately. As each part has different benefits, you can focus on flowers, leaves or roots or make a broad-spectrum extract combining all plant parts of dandelion. But of course, you can put roots, flowers and leaves all together into one beaker and do the extraction from all dandelion parts at the same time.
 

Watch how the probe-type sonicator UP200HT produces a potent dandelion leaf tincture. In this clip, the 200 watts powerful sonicator UP200Ht is used to extract bioactive compounds from fresh dandelion leaves using purified water as a green, mild solvent. Sonication intensifies and accelerates the preparation of potent dandelion tinctures.

Dandelion Leaf Tincture - Achieve High Potency and Efficiency by Sonication

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Materials Needed:

  1. Fresh or dried dandelion leaves, flowers, and roots
  2. Probe-type sonicator, e.g. UP200Ht with S26d14 sonotrode
  3. Solvent (e.g., ethanol, water, or a mixture)
  4. Glass beaker
  5. Filtration setup (e.g., filter paper, funnel)
  6. For concentrated extracts: Rotary evaporator or vacuum evaporator (optional, for solvent removal)
  7. Analytical balance for weighing the ingredients
  8. Ice water bath for temperature control

 

Preparation of the Dandelion Plant:

  • Forage for wild dandelion in an area, where the plant is not polluted by traffic or industrial exhaust. Or buy preferably organically grown dandelion.
  • Wash your plants well to remove all dirt, bugs, etc. Scrub roots carefully with a sponge or a vegetable brush to remove of all dirt.
  • Pat gently dry with a towel.
  • Chop the plant parts into small pieces (approx. 5-10mm particles). This enhances the extraction efficiency significantly.

 
Use the Solvent of Your Choice:
Nettle extraction using cold water and the sonicator UP400ST. Ultrasonic cold water extraction is a very gentle, yet efficient technique to produce high-quality botanical extracts.Choose a solvent appropriate for the target compounds and your extract or tincture. Ethanol-water mixtures are commonly used due to their effectiveness and low toxicity. For an alcohol-free tincture, replace the aqueous ethanol with vinegar (e.g. apple cider vinegar), food-grade glycerine or even use just purified water.
For leaves and flowers, 50% aqueous ethanol (50:50 v/v) is a commonly used solvent that dissolves the bioactive compounds found in dandelion leaves and flowers.
For dandelion roots, 70% aqueous ethanol (30:70 v/v water:ethanol) is often used as the bioactive compounds in dandelion roots dissolve better in a more nonpolar solvent.
Target compounds in dandelion leaves: Flavonoids, vitamins, and minerals.
Target compounds in dandelion flowers: Polyphenols, flavonoids, and antioxidants.
Target compounds in dandelion leaves: Inulin, sesquiterpene lactones, and taraxasterol.
 
Dandelion Leave Extraction using a Probe-Type Sonicator

In this recipe for dandelion leaf extraction, we use 25 gr fresh dandelion leaves per 200mL aqueous ethanol.

  1. Place the plant material in the glass beaker and add the solvent. Ensure the material is fully submerged.
  2. Set the UP200Ht to 100% amplitude and 100% cycle.
  3. Insert the ultrasonic probe into the beaker containing the plant material and solvent. Position the probe to ensure it is submerged but not touching the beaker walls or bottom.
  4. Use the pluggable temperature sensor PT100 (supplied with the sonicator) to monitor the temperature. Maintain it below 40°C using an ice water bath to prevent thermal degradation of compounds.
  5. Sonicate the mixture for approx. 5 min. Move the sonotrode slowly through the mixture to ensure that all plant parts are exposed to the ultrasound waves.

 
Filtration:
After sonication, filter the mixture through filter paper to separate the liquid extract from the plant residues.
 
Solvent Removal (optional):
If necessary, concentrate the extract by removing the solvent using a rotary evaporator or vacuum evaporator.
 
Storage:
Store the extract in a dark glass bottle at a low temperature to preserve its bioactive compounds.
 

Dandelion leaf extraction is most efficiently performed using  a probe-type sonicator.

Sonicator UP200Ht with the ultrasonically extracted dandelion leaf tincture.

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Find the Best Sonicator for Botanical Extraction

Hielscher Ultrasonics offers a wide range of probe-type sonicators for small to large scale botanical extraction. If you select a sonicator for your extraction project, you might consider the following factors as they will impact the outcome of your extraction results.

  1. Versatile Applications:
    Hielscher ultrasonicators are suitable for various extraction processes, including the isolation of essential oils, bioactive compounds, and pigments from plants. This versatility makes them invaluable in the fields of pharmaceuticals, nutraceuticals, cosmetics, and food industries.
  2. Probe-Type vs Bath Sonicator:
    Probe-type sonicators outcompete bath sonicators for extraction due to several critical factors related to their design, functionality, and efficiency. Understanding these advantages provides insights into why probe-type sonicators are preferred for high-performance and large-scale extraction processes. Read more here!
  3. High Efficiency:
    Choose the suitable sonicator and sonotrode to optimize the extraction efficiency. Ultrasonic extraction enhances the yield and quality of botanical extracts by efficiently breaking down plant cell walls, facilitating the release of intracellular compounds. This method is faster and often more effective than traditional extraction techniques.
  4. Process Control:
    Features such as adjustable amplitude, temperature control, continuous + pulsed operation mode, and automatic data protocolling provide precise control over the extraction process, ensuring reproducibility and optimal results.
  5. Scalability:
    The product line ranges from compact, handheld units ideal for small-scale research and pilot studies, to powerful, industrial-grade systems capable of processing large volumes of plant material. This scalability ensures seamless transition from R&D to full-scale production.

 
Our well-trained, long-time experienced technical staff will be glad to consult you regarding the most suitable sonicator fulfilling your botanical extraction needs! Contact us now! We are glad to support you!
 

Why Hielscher Ultrasonics?

  • high efficiency
  • state-of-the-art technology
  • reliability & robustness
  • adjustable, precise process control
  • batch & inline
  • for any volume
  • intelligent software
  • smart features (e.g., programmable, data protocolling, remote control)
  • easy and safe to operate
  • low maintenance
  • CIP (clean-in-place)

Design, Manufacturing and ConsultingQuality Made in Germany

Hielscher ultrasonicators are well-known for their highest quality and design standards. Robustness and easy operation allow the smooth integration of our ultrasonicators into industrial facilities. Rough conditions and demanding environments are easily handled by Hielscher ultrasonicators.

Hielscher Ultrasonics is an ISO certified company and put special emphasis on high-performance ultrasonicators featuring state-of-the-art technology and user-friendliness. Of course, Hielscher ultrasonicators are CE compliant and meet the requirements of UL, CSA and RoHs.

The table below gives you an indication of the approximate processing capacity of our ultrasonicators:

Batch VolumeFlow RateRecommended Devices
1 to 500mL10 to 200mL/minUP100H
10 to 2000mL20 to 400mL/minUP200Ht, UP400St
0.1 to 20L0.2 to 4L/minUIP2000hdT
10 to 100L2 to 10L/minUIP4000hdT
15 to 150L3 to 15L/minUIP6000hdT
n.a.10 to 100L/minUIP16000
n.a.largercluster of UIP16000

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Ultrasonic high-shear homogenizers are used in lab, bench-top, pilot and industrial processing.

Hielscher Ultrasonics manufactures high-performance ultrasonic homogenizers for mixing applications, dispersion, emulsification and extraction on lab, pilot and industrial scale.



 

In this presentation we introduce you to the manufacturing of botanical extracts. We explain the challenges of producing high-quality botanical extracts and how a sonicator can help you to overcome these challenges. This presentation will show you how ultrasonic extraction works. You will learn, what benefits you can expect using a sonicator for extraction and how you can implement an ultrasonic extractor into your extract production.

Ultrasonic Botanical Extraction - How to use sonicators to extract botanical compounds

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Facts Worth Knowing

What are the Most Important Bioactive Compounds in Dandelion?

Dandelion (Taraxacum officinale) contains a variety of bioactive compounds that contribute to its medicinal properties. These bioactive compounds in dandelion include antioxidant, anti-inflammatory, antimicrobial, and anticancer activities. These compounds work synergistically to support various bodily functions and maintain overall health. Understanding these components helps in appreciating the therapeutic potential of dandelion and its use in traditional and modern medicine.Below are some of the most important ones:

  1. Flavonoids
    Flavonoids are a group of polyphenolic compounds known for their antioxidant properties. In dandelion, key flavonoids include:
    Luteolin, which exhibits anti-inflammatory, antioxidant, and anticancer activities.
    Apigenin, that is known for its anti-inflammatory, antioxidant, and chemopreventive properties.
  2. Phenolic Acids
    Phenolic acids in dandelion contribute to its antioxidant and anti-inflammatory effects. These include:
    Chlorogenic acid, which offers antioxidant, anti-inflammatory, and antidiabetic effects.
    Caffeic acid, which exhibits antioxidant, anti-inflammatory, and antimicrobial properties.
  3. Sesquiterpene Lactones
    These compounds are primarily responsible for the bitter taste of dandelion and have notable biological activities, including:
    Taraxacin, which shows potential anti-inflammatory and digestive stimulant effects.
    Taraxasterol, known for its anti-inflammatory, hepatoprotective, and anticancer properties.
  4. Triterpenoids
    Triterpenoids in dandelion are associated with various therapeutic effects:
    Lupeol, which exhibits anti-inflammatory, anticancer, and antimicrobial activities.
    β-Sitosterol, which is known for its cholesterol-lowering and anti-inflammatory effects.
  5. Carotenoids
    Carotenoids, such as beta-carotene, are antioxidants that support overall health:
    Beta-carotene is a precursor to vitamin A, supports vision, immune function, and skin health
  6. Vitamins and Minerals
    Dandelion is rich in several vitamins and minerals:
    Vitamin A: Essential for vision, immune function, and skin health.
    Vitamin C: Supports immune function, skin health, and acts as an antioxidant.
    Vitamin K: Important for blood clotting and bone health.
    Potassium: Crucial for maintaining fluid balance and proper muscle function.
    Calcium and Magnesium: Important for bone health and enzymatic activities.

  7. Inulin
  8. Inulin is a type of prebiotic fiber found in dandelion roots and supports gut health by promoting the growth of beneficial bacteria in the intestines, aids in digestion, and helps regulate blood sugar levels.

What is Dandelion Liquid Extract used for?

Dandelion liquid extract, derived from the roots and leaves of the dandelion plant (Taraxacum officinale), is used in various traditional and modern medical applications due to its diverse range of bioactive compounds. While dandelion liquid extract has been used traditionally for centuries, modern scientific research supports many of its applications. Given its broad spectrum of benefits, it might be worth considering as a natural supplement, particularly for digestive and liver health.
Below are some of the primary uses:

  • Digestive Health
    Dandelion extract is known to stimulate appetite and increase bile production, which can aid digestion. The bitter compounds in dandelion enhance liver and gallbladder function, promoting efficient digestion and helping with conditions like indigestion and constipation.

  • Diuretic Effect
    Often referred to as a natural diuretic, dandelion extract helps the body eliminate excess fluid. This property can be beneficial for reducing water retention and bloating, and it has been traditionally used to support kidney function.
  • Anti-inflammatory and Antioxidant Properties
    Dandelion contains potent antioxidants, including flavonoids, phenolic acids, and coumarins, which help neutralize free radicals and reduce inflammation. These properties make it useful in managing conditions characterized by chronic inflammation, such as arthritis.
  • Liver Health
    Dandelion root extract is commonly used to support liver health. Its role in promoting bile production aids in detoxifying the liver, potentially protecting it from oxidative stress and damage.
  • Skin Health
    The anti-inflammatory and antimicrobial properties of dandelion extract make it useful in treating skin conditions like acne, eczema, and psoriasis. It can be applied topically or consumed to support skin health from within.
  • Blood Sugar Regulation
    Some studies suggest that dandelion extract may help regulate blood sugar levels, making it potentially beneficial for individuals with diabetes or those at risk of developing diabetes. This effect is thought to be due to its influence on insulin production and glucose metabolism.
  • Cancer Research
    Preliminary studies have indicated that dandelion root extract may have anticancer properties. Research shows it might induce apoptosis (programmed cell death) in cancer cells, though more extensive clinical studies are needed to confirm these effects.
  • Immune System Support
    The vitamins (A, C, and K) and minerals (potassium, calcium, magnesium) found in dandelion contribute to overall immune system support. These plant compounds can enhance the body’s ability to fight infections and reduce the risk of chronic diseases.

Literature / References


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