I-Hielscher Ultrasound Technology

Ukuhlukaniselana Kwezintathu Zama-Ultrasonically Enhanced

  • Ukuhlukaniswa kwesigaba sesithathu (TPP) kuyindlela yokukhipha, ukuhlukanisa nokuhlanza izingxenye, isib. I-lipids, ama-enzyme, i-polysaccharides nezinye izinto eziphilayo.
  • I-ultrasonically yasiza ukwahlukana kwezintathu ezintathu kune-TPP evamile ngesivuno esiphezulu, ukuhlanzeka okuthuthukile kanye nejubane elihlukile.
  • I-ultrasonic TPP isebenza ezincwadini ezincane nezinkulu futhi ingafakwa kalula ekukhiqizeni izimboni.

 

I-Ultrasonic Three-Phase Partitoning

Ukuhlukanisa ngezigaba ezintathu

I-phase-phase divisionitioning (TPP) iyindlela elula futhi ephumelelayo yendlela eyodwa yokuhlukanisa, ukuhlanzwa nokuhlushwa kwe-biomolecules ezinjenge-enzyme, lipids, i-polysaccharides njll kusuka ekuxukeni okuyinkimbinkimbi.
I-TPP yenziwa ngokungezwa okulandelanayo okwanele kasawoti (ngokuvamile i-ammonium sulphate) kanye ne-solvent (ikakhulukazi t-butanol /tert-butanol). Amanoni afana ne-ammonium sulfate asetshenziselwa ukugcwalisa okwenziwe nge-saturation ukuze kunciphise amaprotheni. I-solvent ephilayo njengokufana t-butanol yengezwa ukwakha izingqimba ezintathu zesigaba nokukhipha izakhi ezincane zamathambo ezinjenge-lipids, ama-phenolics kanye namanye ama-detergents. Kusukela t-butanol inephuzu eliphakeme lokubilisa, kepha lingaphansi kokushisa kune-ethanol ne-methanol, t-butanol yi-solvent ekhethiwe ye-TPP.
Ngemuva kokwelashwa kwe-extract engcolile kanye ne-decantation, ingxube ihlukanisa ngezigaba ezintathu ezihlukene: i-solvent engenhla (t-butanol) isiqukethe ama-non-polar compounds, ahlukaniswe nesigaba esiphansi sama-aqueous (aqukethe amaphilisi e-polar) nge-protein yokufiphaza. Amaphrotheni ayifunayo ahlukaniswa ngokukhetha kwesigaba esisodwa kanye namanye amaprotheni angcolile kwesinye isigaba. Lokhu kubangela ukuhlanzwa okuyingxenye kanye nokuhlushwa kwamaprotheni. Inqubo yokucubungula ukuhlanganiswa kwe-kosmotropic, i-salting out, i-cosolvent isotonic ne-osmolytic precipitation yamaprotheni.

Isicelo solwazi




Qaphela kwethu Inqubomgomo yobumfihlo.


I-TPP Enamandla Ethuthukisiwe

I-Ultrasonic Three-Phase Partitioning ihamba phambili ngokuhlanzeka okukhulu, ukutakula nokunciphisa isikhathi sokucubungula.Sonication intensifies the TPP process significantly. Ultrasonically assisted TPP is successfully applied for the separation and purification of biomolecules. A wide range of biomoelcules have been isolated more efficiently in shorter time. For instance, enzymes from plants (e.g. bromelain, papain, invertase, polyphenol oxidase and trypsin inhibitors), animals (e.g. trypsin, α-chymotrypsin, chymosin, pepsin and luciferase) and microbes (e.g. lipase, α-amylase, α-galactosidase, serratiopeptidase, cyclodextrin glycosyltransferase, and fibrinolytic enzymes) were purified via ultrasonic TPP. An additional benefit of the ultrasonic TPP is the enhanced activity of various enzymes, resulting in apparent higher yields (>100%) since the targeted enzyme or protein precipitates out in the middle layer between the organic and aqueous phases.
I-ultrasonic TPP ingasetshenziswa ngokuphumelelayo ngamasampuli angcolile futhi ingafakwa kalula.

I-Ultrasonically ithuthukise ukuhlukaniswa kwesigaba sesithathu (TPP)

I-Sonication ithuthukisa ukuhlukaniswa kwesigaba sesithathu ngokuphawulekayo futhi kubangelwa ukuvunwa okuphakeme kanye nokuhlanzeka ngesikhathi esifushane kakhulu senqubo.

Izinzuzo:

  • isivuno esiphezulu
  • ukululama ngokushesha
  • ukuhlanzeka okukhulu
  • ukugcina isikhathi
  • ayabizi
  • kulula ukugijima
  • i-eco-friendly
  • for lab kanye nembonini

 

Izibonelo ze-US-TPP

I-ultrasonic TPP ye-extraction ye-astaxanthin

Ukukhishwa kwe-astaxanthin (AX), ukwelashwa okuhlanganyelwe kwe-TPP nokukhulisa nge-Hielscher's UP400S akuyona nje kuphela i-elula elula esebenzayo, kodwa futhi futhi inqubo ephumelelayo kakhulu ukuhlukanisa i-AX kusuka ku-biomass ye-bacteria ye-Paracoccus NBRC 101723. Ukuhlukahluka kokusebenza kwe-sonication kungenziwa kahle ukuze uthole imiphumela emihle yokukhipha. Ama-ultrasonic processing parameters auch njenge-amplitude, isikhathi, izinga lokushisa kanye nesitokisi (isayizi, ukuma) kanye nobukhulu bezinhlayiyana ze-biomass, kuthinta ukukhululwa kwe-ultrasonic kwe-AX kusuka ku-biomass kakhulu. I-Ultrasonication ye-biomass emanzi inikeza imiphumela engcono kune TPP ye-ultrasonic ye-biomass eyomile yokufakelwa okusheshayo nephumelelayo kwe-AX. I-TPP ye-ultrasonic iholela ekutholeni okungaphezulu kwe-37% ephakeme kwe-AX kune-extraction evamile yokusungula. (cf. Chougle et al. 2013)

I-Ultrasonic TPP yokuhlukaniswa kwe-andrographolide evela ku-Andrographis paniculata

I-Andrographolide iyinhlangano ye-labdane diterpenoid, ebizwa ngokuthi i-anti-inflammatory, antioxidant, i-hepatoprotective, i-anti-carcinogenic kanye ne-anti-diabetes. Ucwaningo oluqhathanisa lubonisa ukuthi i-TPP ye-ultrasonic ayikwenyanga nje isivuno se-andrographolide kodwa iphinde ithuthukise umsebenzi we-antioxidative uma kuqhathaniswa ne-TPP evamile.

Ukusebenza okuphezulu kwe-Ultrasonic Processors

I-Hielscher Ultrasonics iyazikhethela ekuklanyeni nasekukhiqizeni izinhlelo eziphezulu ze-ultrasonic ze-extraction kanye nezinye izinqubo eziningi.
Ibanga lethu lomkhiqizo lihlanganisa amancane, amadivayisi anamathuluzi anamandla, ezinamandla ze-bench-top ultrasonicators kanye izinhlelo eziphezulu zokusebenza ezimbonini. Okuhlukahlukene Izesekeli vumela i-adaption ekahle yesistimu ye-ultrasonic kuya ezidingweni zakho ezithile zokusebenza. Wonke ama-ultrasonicators ama-Hielscher akhiwe ngomsebenzi we-24/7 ngaphansi komthwalo ogcwele.
Okuhlangenwe nakho kwethu isikhathi eside ekusetshenzisweni kwe-ultrasonic kusivumela ukuthi sixoxisane namakhasimende ethu ekuhlolweni kwawo kokuqala nge-ultrasound ngokusebenzisa izinyathelo zenqubo yokusebenza nokwenza kusetshenziswa izilinganiso zezimboni.
Sicela usebenzise ifomu ngezansi ukusitshela ngenqubo yakho! Siyajabula ukuncoma ukuthi sixoxe ngamathuba nezinzuzo ze-sonication kwesicelo sakho.

Xhumana nathi! / Cela Us!

Sicela usebenzise ifomu elingezansi, uma ufisa ukucela ulwazi oluthe xaxa mayelana ne-homogenization yama-ultrasonic. Sizojabula ukukunikeza uhlelo lwe-ultrasonic ukuhlangabezana nezidingo zakho.









Sicela uqaphele wethu Inqubomgomo yobumfihlo.


I-Hielscher ihlanganisa ububanzi obugcwele kusukela kubumbano be-compact lab ultrasonicators kuze kube ngosayizi we-bench-top kanye nezinhlelo ezigcwele zokukhiqiza kwezohwebo.

Imishini esebenzayo futhi enokwethenjelwa ye-ultrasonic kusukela ebhodini kuya esikalini semakethe.

Izincwadi / Izinkomba

  • I-Chougle, i-JA: et al. (2013): I-Ultrasound yasize ukuhlukaniswa kwesigaba sesithathu (UATPP): Inqubo yombhalo eyenzelwe ukukhishwa kwe-astaxanthin evela eParacoccus NBRC 101723. CSBE / SCGAB 2013 – Annual Conference University of Saskatchewan, Saskatoon, 7-10 Julayi 2013.
  • Ketnawa, S. et al (2017): Isigaba sokuhlukanisa ukuhlukaniswa kwe-enzyme: Ukubuka konke kanye nezinhlelo zokusebenza zakamuva. I-International Food Research Journal 24 (1), 2017. 1-24.
  • UVarakumar, S. et al. (2017): Isiqhamo esithuthukisiwe se-oleoresin esivela ku-ginger (i-Zingiber officinale) i-rhizome powder esebenzisa i-enzyme-esasizwa ngezigaba ezintathu zokuhlukanisa. I-Chemistry yokudla 216, 2017. 27-36.
  • Yan J.-K. et al. (2018): I-Ultrasound isebenzisane ngezigaba ezintathu zesigaba sokuhlukanisa nokuhlukaniswa kwe-Corbicula fluminea polysaccharides nezinqubo ezifanelekayo. Ultrasonics Sonochemistry Vol. 40, Ingxenye A, 2018. 128-134.


Amaqiniso Okufanele Ukwazi

I-Part-Phase Partitioning (TPP)

I-TPP iyindlela yokwenza izinto, ngokusekelwe ekuhlukaniseni izingxenye ze-polar, amaprotheni, nama-hydrophobic ezigaba ezintathu ezihlanganisa amanzi, i-ammonium sulphate ne-t-butanol.
Izakhi ezinomsoco ezibalulekile kanye nezimboni (isib. Α-galactocidase, α-amylase inhibitors, kanye ne-protease) nezinhlanganisela zemvelo (isib. I-diterpenes efana ne-forskolin ne-andrographolide) zihlanzwa yizigaba ezintathu zokuhlukanisa. Nakuba izingulube, i-lipids kanye ne-enzyme inhibitors ziqoqa esigabeni esingenhla se-solvent, esahlukaniswa nesigaba esiphansi sama-aqueous, lapho kuhlanganiswa khona izingxenye ze-polar ezinjenge-saccharides, ngezingqimba ezinamaprotheni eziphakathi. Kusukela t-butanol ibopha amaprotheni akhankanywe yi-TPP, okwenzeka njengama-protein-t-butanol coprecipitates, afaka phakathi kwezingqimba eziphilayo futhi aqueous futhi angahlukaniswa kalula futhi ahlanzeke. Ngokuvamile, ama-biomolecules atholakala ngendlela ehlanzekile e-interphase, kuyilapho izidakamizwa ikakhulukazi zihlukanisa t-butanol (isigaba esiphakeme) nasesigaba se-aqueous (isigaba esiphansi).

Astaxanthin

Astaxanthin (3, 3´-dihydroxy-ß, ß-carotene 4, 4´-dione) is a keto-carotenoid that belongs to the class of terpenes. As tetraterpenoid it is built from five carbon precursors, isopentenyl diphosphate, and dimethylallyl diphosphate. Astaxanthin is a yellow-orange plant pigmant and therefore classified as a xanthophyll. Astaxanthin is mainly found in microalgae, yeast, salmonoids, trout, krill, shrimp, crayfish, and crustaceans. As an highly potent free radical scavenger, skin protectant, anticarcinogenic and as an enhancer of immune system, astaxanthin is used as supplement and as additive in foods, pharmaceuticals and cosmetics. Ultrasonic TPP improves the extraction and separation process of astaxanthin from natural sources and makes its economical production applicable to industrial level.

I-Hielscher Ultrasonics ikhethekile ekusetshenzisweni okuphezulu kwe-ultrasonic

I-UIP1000hdT – 1kW ultrasonicator yezimboni