I-Hielscher Ultrasound Technology

Ukuhlukaniselana Kwezintathu Zama-Ultrasonically Enhanced

  • Ukuhlukaniswa kwesigaba sesithathu (TPP) kuyindlela yokukhipha, ukuhlukanisa nokuhlanza izingxenye, isib. I-lipids, ama-enzyme, i-polysaccharides nezinye izinto eziphilayo.
  • I-ultrasonically yasiza ukwahlukana kwezintathu ezintathu kune-TPP evamile ngesivuno esiphezulu, ukuhlanzeka okuthuthukile kanye nejubane elihlukile.
  • I-ultrasonic TPP isebenza ezincwadini ezincane nezinkulu futhi ingafakwa kalula ekukhiqizeni izimboni.

I-Ultrasonic Three-Phase Partitoning

Ukuhlukanisa ngezigaba ezintathu

I-phase-phase divisionitioning (TPP) iyindlela elula futhi ephumelelayo yendlela eyodwa yokuhlukanisa, ukuhlanzwa nokuhlushwa kwe-biomolecules ezinjenge-enzyme, lipids, i-polysaccharides njll kusuka ekuxukeni okuyinkimbinkimbi.
I-TPP yenziwa ngokungezwa okulandelanayo okwanele kasawoti (ngokuvamile i-ammonium sulphate) kanye ne-solvent (ikakhulukazi t-butanol /tert-butanol). Amanoni afana ne-ammonium sulfate asetshenziselwa ukugcwalisa okwenziwe nge-saturation ukuze kunciphise amaprotheni. I-solvent ephilayo njengokufana t-butanol yengezwa ukwakha izingqimba ezintathu zesigaba nokukhipha izakhi ezincane zamathambo ezinjenge-lipids, ama-phenolics kanye namanye ama-detergents. Kusukela t-butanol inephuzu eliphakeme lokubilisa, kepha lingaphansi kokushisa kune-ethanol ne-methanol, t-butanol yi-solvent ekhethiwe ye-TPP.
Ngemuva kokwelashwa kwe-extract engcolile kanye ne-decantation, ingxube ihlukanisa ngezigaba ezintathu ezihlukene: i-solvent engenhla (t-butanol) isiqukethe ama-non-polar compounds, ahlukaniswe nesigaba esiphansi sama-aqueous (aqukethe amaphilisi e-polar) nge-protein yokufiphaza. Amaphrotheni ayifunayo ahlukaniswa ngokukhetha kwesigaba esisodwa kanye namanye amaprotheni angcolile kwesinye isigaba. Lokhu kubangela ukuhlanzwa okuyingxenye kanye nokuhlushwa kwamaprotheni. Inqubo yokucubungula ukuhlanganiswa kwe-kosmotropic, i-salting out, i-cosolvent isotonic ne-osmolytic precipitation yamaprotheni.

Isicelo solwazi




Qaphela kwethu Inqubomgomo yobumfihlo.


I-TPP Enamandla Ethuthukisiwe

I-Ultrasonic Three-Phase Partitioning ihamba phambili ngokuhlanzeka okukhulu, ukutakula nokunciphisa isikhathi sokucubungula.I-Sonication iqinisa inqubo ye-TPP kakhulu. I-TPP esebenzisa i-ultrasonically isebenza ngokuphumelelayo ekuhlukaneni nasekuhlanjululeni kwe-biomolecules. Ama-biomoelcules ahlukahlukene ahlukaniswe kahle kakhulu ngesikhathi esifushane. Isibonelo, ama-enzyme ezitshalweni (isib. I-bromelain, i-papain, i-invertase, i-polyphenol oxidase kanye ne-trypsin inhibitors), izilwane (isib. Trypsin, i-a-chymotrypsin, i-chymosin, i-pepsin ne-luciferase) namagciwane (isib. Lipase, α-amylase, α-galactosidase, i-serratiopeptidase, i-cyclodextrin glycosyltransferase, nama-enzyme e-fibrinolytic) ahlanzwa nge-TPP ultrasonic. Inzuzo eyengeziwe ye-TPP ye-ultrasonic iyisenzo esithuthukisiwe se-enzyme ehlukahlukene, okuholela ekuvuneni okuphezulu okubonakalayo (> 100%) kusukela i-enzyme ehlosiwe noma amaprotheni inqamuka phakathi kocingo oluphakathi phakathi kwezigaba eziphilayo kanye nama-aqueous.
I-ultrasonic TPP ingasetshenziswa ngokuphumelelayo ngamasampuli angcolile futhi ingafakwa kalula.

I-Ultrasonically ithuthukise ukuhlukaniswa kwesigaba sesithathu (TPP)

I-Sonication ithuthukisa ukuhlukaniswa kwesigaba sesithathu ngokuphawulekayo futhi kubangelwa ukuvunwa okuphakeme kanye nokuhlanzeka ngesikhathi esifushane kakhulu senqubo.

Izinzuzo:

  • isivuno esiphezulu
  • ukululama ngokushesha
  • ukuhlanzeka okukhulu
  • ukugcina isikhathi
  • ayabizi
  • kulula ukugijima
  • i-eco-friendly
  • for lab kanye nembonini

Izibonelo ze-US-TPP

I-ultrasonic TPP ye-extraction ye-astaxanthin

Ukukhishwa kwe-astaxanthin (AX), ukwelashwa okuhlanganyelwe kwe-TPP nokukhulisa nge-Hielscher's UP400S akuyona nje kuphela i-elula elula esebenzayo, kodwa futhi futhi inqubo ephumelelayo kakhulu ukuhlukanisa i-AX kusuka ku-biomass ye-bacteria ye-Paracoccus NBRC 101723. Ukuhlukahluka kokusebenza kwe-sonication kungenziwa kahle ukuze uthole imiphumela emihle yokukhipha. Ama-ultrasonic processing parameters auch njenge-amplitude, isikhathi, izinga lokushisa kanye nesitokisi (isayizi, ukuma) kanye nobukhulu bezinhlayiyana ze-biomass, kuthinta ukukhululwa kwe-ultrasonic kwe-AX kusuka ku-biomass kakhulu. I-Ultrasonication ye-biomass emanzi inikeza imiphumela engcono kune TPP ye-ultrasonic ye-biomass eyomile yokufakelwa okusheshayo nephumelelayo kwe-AX. I-TPP ye-ultrasonic iholela ekutholeni okungaphezulu kwe-37% ephakeme kwe-AX kune-extraction evamile yokusungula. (cf. Chougle et al. 2013)

I-Ultrasonic TPP yokuhlukaniswa kwe-andrographolide evela ku-Andrographis paniculata

I-Andrographolide iyinhlangano ye-labdane diterpenoid, ebizwa ngokuthi i-anti-inflammatory, antioxidant, i-hepatoprotective, i-anti-carcinogenic kanye ne-anti-diabetes. Ucwaningo oluqhathanisa lubonisa ukuthi i-TPP ye-ultrasonic ayikwenyanga nje isivuno se-andrographolide kodwa iphinde ithuthukise umsebenzi we-antioxidative uma kuqhathaniswa ne-TPP evamile.

Ukusebenza okuphezulu kwe-Ultrasonic Processors

I-Hielscher Ultrasonics iyazikhethela ekuklanyeni nasekukhiqizeni izinhlelo eziphezulu ze-ultrasonic ze-extraction kanye nezinye izinqubo eziningi.
Ibanga lethu lomkhiqizo lihlanganisa amancane, amadivayisi anamathuluzi anamandla, ezinamandla ze-bench-top ultrasonicators kanye izinhlelo eziphezulu zokusebenza ezimbonini. Okuhlukahlukene Izesekeli vumela i-adaption ekahle yesistimu ye-ultrasonic kuya ezidingweni zakho ezithile zokusebenza. Wonke ama-ultrasonicators ama-Hielscher akhiwe ngomsebenzi we-24/7 ngaphansi komthwalo ogcwele.
Okuhlangenwe nakho kwethu isikhathi eside ekusetshenzisweni kwe-ultrasonic kusivumela ukuthi sixoxisane namakhasimende ethu ekuhlolweni kwawo kokuqala nge-ultrasound ngokusebenzisa izinyathelo zenqubo yokusebenza nokwenza kusetshenziswa izilinganiso zezimboni.
Sicela usebenzise ifomu ngezansi ukusitshela ngenqubo yakho! Siyajabula ukuncoma ukuthi sixoxe ngamathuba nezinzuzo ze-sonication kwesicelo sakho.

Xhumana nathi! / Cela Us!

Sicela usebenzise ifomu elingezansi, uma ufisa ukucela ulwazi oluthe xaxa mayelana ne-homogenization yama-ultrasonic. Sizojabula ukukunikeza uhlelo lwe-ultrasonic ukuhlangabezana nezidingo zakho.









Sicela uqaphele wethu Inqubomgomo yobumfihlo.


I-Hielscher ihlanganisa ububanzi obugcwele kusukela kubumbano be-compact lab ultrasonicators kuze kube ngosayizi we-bench-top kanye nezinhlelo ezigcwele zokukhiqiza kwezohwebo.

Imishini esebenzayo futhi enokwethenjelwa ye-ultrasonic kusukela ebhodini kuya esikalini semakethe.

Izincwadi / Izinkomba

  • I-Chougle, i-JA: et al. (2013): I-Ultrasound yasize ukuhlukaniswa kwesigaba sesithathu (UATPP): Inqubo yombhalo eyenzelwe ukukhishwa kwe-astaxanthin evela eParacoccus NBRC 101723. CSBE / SCGAB 2013 – Annual Conference University of Saskatchewan, Saskatoon, 7-10 Julayi 2013.
  • Ketnawa, S. et al (2017): Isigaba sokuhlukanisa ukuhlukaniswa kwe-enzyme: Ukubuka konke kanye nezinhlelo zokusebenza zakamuva. I-International Food Research Journal 24 (1), 2017. 1-24.
  • UVarakumar, S. et al. (2017): Isiqhamo esithuthukisiwe se-oleoresin esivela ku-ginger (i-Zingiber officinale) i-rhizome powder esebenzisa i-enzyme-esasizwa ngezigaba ezintathu zokuhlukanisa. I-Chemistry yokudla 216, 2017. 27-36.
  • Yan J.-K. et al. (2018): I-Ultrasound isebenzisane ngezigaba ezintathu zesigaba sokuhlukanisa nokuhlukaniswa kwe-Corbicula fluminea polysaccharides nezinqubo ezifanelekayo. Ultrasonics Sonochemistry Vol. 40, Ingxenye A, 2018. 128-134.


Amaqiniso Okufanele Ukwazi

I-Part-Phase Partitioning (TPP)

I-TPP iyindlela yokwenza izinto, ngokusekelwe ekuhlukaniseni izingxenye ze-polar, amaprotheni, nama-hydrophobic ezigaba ezintathu ezihlanganisa amanzi, i-ammonium sulphate ne-t-butanol.
Izakhi ezinomsoco ezibalulekile kanye nezimboni (isib. Α-galactocidase, α-amylase inhibitors, kanye ne-protease) nezinhlanganisela zemvelo (isib. I-diterpenes efana ne-forskolin ne-andrographolide) zihlanzwa yizigaba ezintathu zokuhlukanisa. Nakuba izingulube, i-lipids kanye ne-enzyme inhibitors ziqoqa esigabeni esingenhla se-solvent, esahlukaniswa nesigaba esiphansi sama-aqueous, lapho kuhlanganiswa khona izingxenye ze-polar ezinjenge-saccharides, ngezingqimba ezinamaprotheni eziphakathi. Kusukela t-butanol ibopha amaprotheni akhankanywe yi-TPP, okwenzeka njengama-protein-t-butanol coprecipitates, afaka phakathi kwezingqimba eziphilayo futhi aqueous futhi angahlukaniswa kalula futhi ahlanzeke. Ngokuvamile, ama-biomolecules atholakala ngendlela ehlanzekile e-interphase, kuyilapho izidakamizwa ikakhulukazi zihlukanisa t-butanol (isigaba esiphakeme) nasesigaba se-aqueous (isigaba esiphansi).

Astaxanthin

I-Astaxanthin (i-3, 3'-dihydroxy-ß, i-ß-carotene 4, 4'-dione) i-keto-carotenoid eseklasini lama-terpenes. Njengoba i-tetraterpenoid yakhiwa kusuka kubakhiqizi abahlanu be-carbon, i-isopentenyl diphosphate, ne-dimethylallyl diphosphate. I-Astaxanthin isitshalo se-yellow-orange pigmant ngakho-ke sihlukaniswe njenge-xanthophyll. I-Astaxanthin itholakala ngokuyinhloko kwi-microalgae, imvubelo, i-salmonoids, i-trout, i-krill, i-shrimp, i-crayfish nama-crustaceans. Njengomkhuhlane onamandla okhululekile, isikhumba esivikela isikhumba, i-anticarcinogenic kanye nesisusa somzimba wamasosha omzimba, i-astaxanthin isetshenziselwa ukwengeza futhi njengokwengeza kokudla, imithi kanye nezimonyo. I-ultrasonic TPP ithuthukisa inqubo yokuhlukanisa nokuhlukaniswa kwe-astaxanthin emithonjeni yemvelo futhi yenza ukukhiqizwa kwayo okwakhiqiza okusebenza ezingeni lezimboni.

I-Hielscher Ultrasonics ikhethekile ekusetshenzisweni okuphezulu kwe-ultrasonic

I-UIP1000hdT – 1kW ultrasonicator yezimboni