I-Hielscher Ultrasound Technology

Ultrasonic Tissue Homogenizers

 

 

I-homogenization yama-tishu iyinqubo yokulungiselela isampula lapho amaseli wesilwane nesitshalo noma ama-microorganisms ekulungiswa ngaphambi kokukhishwa kwendaba ye-intracellular njengama-protein, i-DNA noma i-RNA. I-membran yeseli kufanele iphulwe ukuze ikhulule okuqukethwe kweli cell. Ngemuva kokuphula udonga lwamaseli, ama-macromolecules angenangqamuzana athamela kwisisombululo se-buffer ukuze i-organelles, amaprotheni ne-DNA / i-RNA itholakale. Ama-homogenizers we-Ultrasonic namasilensi weseli ngamathuluzi asebenza kahle kakhulu cell ukuphazanyiswa, lysis futhi Ukukhipha.

I-homogenizers ye-Ultrasonic isetshenziswa ngokujwayelekile ukuze izicubu zigaya, ukuphazanyiswa kwamaseli & i-lysis kanye ne-homogenization yamasampuli wezinto eziphilayo ngaphambi kokukhishwa kwendaba ye-intracellular kuleso sikhala samangqamuzana. Nge-sonication elawulwayo yamasampuli, zonke izinyathelo ezivela ku-lysis kuya kokukhipha kanye nokwenziwa kwe-homogenization zingenziwa ngokusebenzisa i-ultrasonic cell disruptor efanayo.
Inzuzo enkulu ye homogenizers ultrasonic ilele kalula amandla aguquguqukayo ukufaka nokuqina kwe-sonication. Ama-ultrasonic cell disruptors (i-lysers) avumela ukulawula amandla we-sonication ngokulungisa ukuphakama kusuka ku-20% ukuya ku-100%.
Ngaphandle kokusebenza okuqhubekayo, i-homogenizer ye-ultrasonic ingasethwa ukujikeleza imodi, isb for sonication of izicubu ezibucayi. I-Sonication kumodi ye-pulse kusho i-ultrasonicator edala amagagasi aphezulu kakhulu e-ultrasonic kumjikelezo wezinkathi. Ubude besikhathi sokunikezwa kwesikhathi kanye nesikhathi esithile kungenziwa kubekwe ngabanye ngabasebenzisi.
AT I-Sonication ene-Pulsed, phakathi nesikhathi sokuphumula, izinto eziphathekayo zingabuyela esimweni sayo sokuphumula futhi isampuli inganciphisa (usebenzisa isikhathi esithile sokushisa ukushisa). Ngaleyo ndlela, ukucubungula kahle kungasethwa ngokunembile ngokuhambisana nezicubu kanye nokushisa kwesampula okungadingeki kuncishisiwe.
Ama-homogenizers ama-ultrasonic amancane futhi asebenzise umsebenzisi ngenkathi eveza imiphumela enokwethenjelwa eqinisekisayo ukukhiqizwa kabusha ngenxa yokulawula okugcwele kuzo zonke izinqubo ezibalulekile zenqubo.
Izicelo ezivamile zezigaba ze-ultrasonic izicubu zokugaya, ukuphazanyiswa kwamangqamuzana, i-lysis, i-extraction kanye ne-homogenization zitholakala kuma-laboratories eziphilayo nezidakamizwa, i-microbiology, i-proteomic kanye ne-genomic ucwaningo (isib. Amaprotheni ukukhululwa ngaphambi kwe-Western blot kanye ne-enzyme-linked-immunosorbent assay), kodwa futhi ekukhiqizeni of ukukhishwa futhi amakhemikhali asebenzayo ngokuba izimboni zokudla, i-pharma nezimonyo.

Thola idivayisi ephezulu ye-ultrasonic yezidingo zakho:

Hielscher ultrasonic izicubu homogenizers ziyatholakala nge ultrasound okukhipha uhla phakathi 50W kuya 16,000W (16kW).
Abasebenzi beLatroli bayathokozisa ngokusebenza okulula futhi okunembile kwama-homogenizers we-Hielscher's ultrasonic lab. Kuye ngezidingo ezithile, amadivaysi e-Hielscher afika ngokulawula kwe-digital noma analog.
I I-VialTweeter iyisetshenziswa esinamandla se-ultrasonic evumela ukuthi i-sonication yesikhathi esisodwa ibe nezikhumba ezifika ku-10 noma ama-tubes wokuhlola ngendlela efanayo. Ngenxa yokungena okungaqondile, ukungcola okuphambene nokuphulukiswa kwesampuli kugwenywe ngokuphelele. Ukuhlanza nokuhlanza kuncishiswe kuncishiswe ngenxa yokusetshenziswa kwezigqoko ezilahlwayo. I-sonotrode ye-block ikwazi ukuzenzekela.
Kububanzi be-probe-uhlobo lab ultrasonicators, amadivayisi angakhethwa 50W, 100W, 200W, 250W futhi 400W.
Ukuze uthole izinqubo ezinkulu, izinsizakalo ze-bench-top ultrasonic ziyatholakala nazo 500W, 1000W, 1500W futhi 2000W.
Futhi ngenxa yemifula ephezulu yamanzi, ama-ultrasonicators of 4000W, 10,000W noma 16,000W zihlanganiswa futhi ngaleyo ndlela zikwazi ukucubungula futhi zenze i-homogenize cishe yonke inani lezinhlamvu zeselula.

Izinzuzo ezinkulu zama Ultrasonicators kaHielscher:

 

 

Chofoza lapha ukuze uthole izincomo zenkambiso ethile mayelana nokulungiselela isampula, i-homogenization yezicubu, i-lysis kanye ne-extraction (isb. Amangqamuzana, amabhaktheriya, ama-spores, ama-compounds asebenzayo)!

Xhumana Nathi / Cela Ukwaziswa Olwengeziwe

Khuluma nathi mayelana nezidingo zakho zokucubungula. Sizosikisela ukusetha nokulungiswa kwemingcele efanele yephrojekthi yakho.





Sicela uqaphele wethu Inqubomgomo yobumfihlo.



Ukuqeda amandla kuyisicelo esivamile se-ultrasonication. Le vidiyo ikhombisa ukuqedwa okusheshayo kwamakhristalu kashukela emanzini kusetshenziswa iHielscher UP200St.

Ukuqumba Amakristalu Ushukela Emanzini usebenzisa i-UP200St


Amaqiniso Okufanele Ukwazi

I-ultrasonic izicubu ze-homogenizers ngokuvamile ibizwa ngokuthi i-sonicator yesondlo, i-lyser ye-sonic, i-ultrasound ephazamisayo, i-grinder ye-ultrasonic, i-sono-ruptor, i-sonifier, i-sonic dismembrator, i-cell disrupter, i-ultrasonic disperser noma i-dissolver. Amagama ahlukene avela ezinhlobonhlobo zokusebenza ezingagcwaliseka nge-sonication.