I-Hielscher Ultrasound Technology

Ultrasonic Sample Ukulungiselela for Analysis THC

  • Isizinda se-ultrasonic iyindlela eqinisekisiwe yokukhiqizwa kwe-THC, kodwa ingasetshenziselwa izinhloso zokuhlaziya, futhi.
  • I-ultrasonication iyindlela ejwayelekile yesandulela se-prep esetshenziselwa ukuhlaziywa kokuqukethwe okuphelele kwe-THC kwimikhiqizo ye-cannabis.
  • Isizinda se-ultrasonic sisetshenziselwa amasampuli ngaphambi kokuhlaziywa kwe-TLC, GC ne-HPLC.

Ultrasonic THC Extraction

I-THC, noma i-tetrahydrocannabinol (i-delta-9-tetrahydrocannabinol), iyimithi yamakhemikhali ebangela iningi lomphumela wengqondo yengane. Ngezinhlobo ze-cannabis, zingaba nezinkinga ezingaphezu kuka-400 ezahlukahlukene. Kuye ngokuthi uhlobo oluthile lwe-cannabis, i-cannabis ingaba no-0.3 wt% THC. Kwezinye izinkinga, okuqukethwe kwe-THC kungafinyelela ku-20 wt%.
Futhi imikhiqizo ehlukene ye-cannabis ihlukahluka kakhulu: Ngakho ihlukanisa ukuhlushwa kwe-THC emanzini phakathi kuka-1 kuya ku-5%; ku-hashish, okuqukethwe kwe-THC kuphakathi kuka-5 kuya kwezingu-15%, futhi kumafutha we-hashish, okuyi-20%. I-THC ekudleni kokungcola kwe-insangu iyashintsha kakhulu futhi yehlisa okuqukethwe kwe-THC emanzini, lapho umsebenzisi engadliwa khona ukuze ahlangabezane nemiphumela oyifunayo.
Ukusetshenziswa kwamandla ultrasound (owaziwa nangokuthi sonification) iyithuluzi eliqinisekisiwe lokukhipha izithako ezisebenzayo ezivela emthini wesitshalo. I-THC kanye nezinye izingxenye ezisebenzayo ezifana ne-cannabinoids kanye ne-terpenes zingadluliswa ngempumelelo nge-sonication. Chofoza lapha ukuze ufunde kabanzi mayelana nesithwathwa se-ultrasonic.

Ukulungiswa kwesampula ye-Ultrasonic kusetshenziselwa ukuhlaziya okuqukethwe kwe-THC

  • ukulawulwa kwekhwalithi yemikhiqizo ye-THC yokuzijabulisa,
  • ukuzimisela ikhwalithi yemikhiqizo ye-THC yemithi,
  • ukuhlukaniswa kwezidakamizwa-hemp kanye ne-fiber-hemp.

Ukuhlaziywa kwe-THC, izindlela ezilandelayo ze-chromatographic zivame ukusetshenziswa: I-Layer Chromatography (TLC), i-Gesi Chromatography (GC), ne-High-Performance Liquid Chromatography (HPLC)

Isakhiwo sefomula ye-Δ9-THC yokuhlaziya nokukhipha

I-Tetrahydrocannabinol (Δ9-THC) yokuhlaziya nokukhipha

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I-Sonification iyisinyathelo esivamile ngaphambi kokuhlaziywa (isib. GC, TLC, HPLC)

I-VialTweeter for ultrasonic isampula prep

I-Ultrasonic Sample Prep Protocol ye-TLC Analysis

Ukulungiswa kwesampula: Ukulungiselela ama-sampuli, izinto ezitshaliwe zezitshalo kufanele zifakwe emanzini futhi zigalelwe i-powder ehambisanayo. Isizinda se-ultrasonic, i-500mg ye-cannabis plant powder kanye ne-5mL methanol-chloroform ingxube (isilinganiso se-9: 1) ixutshwe kwi-vial. I-Sonication ingasetshenziswa ngokusebenzisa i-an I-Ultrasonicator ngephuzu elincane, i I-VialTweeter noma i-ultrasonic CupHorn. Isikhathi sonication kuthatha approx. 5 iminithi. Ngemuva kwalokho, vumela isampula ukuphumula okungenani ama-5 min. Nciphisa i-supernatant ecacile ngxube le-extraction (v / v: 1: 10). I-THC yamahhala: 1μL kwi-TLC-plate
I-Decarboxylation: i-100μL ye-extract ikhuphuka ibe yomile. Okusele kushisa ngo-180 ℃ ngamaminithi angu-4. Engeza i-500μL ye-methanol-chloroform (v / v 9: 1). Sonicate for 5 min. Nciphisa i-supernatant ecacile ngxube le-extraction (v / v: 1: 10).
Ukulinganisa: 20ng, 60ng, 100ng, 140ng
I-HPTLC:
I-TLC ye-silica gel F-254, i-Merck 5729
I-TLC scanner II, i-software CATS3, i-Camag Muttenz Eluens:
I-Ethylacetat: Tolol: Chloroform = 3: 17: 0,25 (v / v / v)
I-derivatisation ye-Postchromatographic: I-Blue Blue Salt Salt 0,1% ku-0,1 M NaOH
I-densitometry ku-550 nm

I-Ultrasonic Sample Prep Protocol ye-GC Analysis

Ukulungiselela isampula: Izinto ezitshaliwe ezitshaliwe ziyi-macerated futhi zigalelwe i-powder ehambisanayo. Ngokwesikhumba, i-500mg ye-cannabis plant powder kanye ne-10mL methanol-hexan ingxube (isilinganiso se-9: 1) ixutshwe kwi-vial. I-Sonication ingasetshenziswa ngokusebenzisa i-a i-probe-uhlobo lwe-ultrasonic device ngephuzu elincane, i I-VialTweeter noma i-ultrasonic CupHorn. Khombisa isampula ngemaminithi angu-15-20. Shake isampula bese uyivumela ukuthi iphumule okungenani ama-5 min. Nciphisa i-supernatant ecacile nenhlanganisela yesikhumba (v / v 1: 20).
I-Decarboxyliation: kufakwa
Injector temperatur 250 ℃
Ukulinganisa: 5 ng / μL kuya kuma 250 ng / μL
I-GC: HP 6890 nge-5973 MSD no-7683 i-autosampler injection: 1μL, isikhathi esingenasici: 2 iminithi.
Ikholomu: HP-5 MS, 30m, ububanzi 250 μm, ubukhulu befilimu 0,25 μm
Igesi lensiza: Yena, 35 psi, ukugeleza okuqhubekayo: 4 mL / min.
Ihhavini: 100 ℃ for 1min ;; izinga lokushisa lokushisa lika-15 ℃ / iminithi. kuya ku-280 ℃; 280 ℃ ngamaminithi angu-5. I-MSD: SCAN: m / e 35 – 550

Sonication yisinyathelo esibalulekile ngesikhathi sokulungiselela isampula

UP200St nge-tip-micro ye-sonication yesampula

I-Ultrasonic Sample Prep Protocol ye-HPLC Analysis

Ukulungiswa kwesampula (okufana ne-GC prep): Izinto ezitshaliwe zitshalo zifakwe futhi zigayeke nge-powder ehambisanayo. Ngokwesikhumba, i-500mg ye-cannabis plant powder kanye ne-10mL methanol-hexan ingxube (isilinganiso se-9: 1) ixutshwe kwi-vial. I-Sonication ingasetshenziswa ngokusebenzisa i-a I-Probe-type ultrasonicator ngephuzu elincane, i I-VialTweeter noma i-ultrasonic CupHorn. Khombisa isampula ngemaminithi angu-15-20. Shake isampula bese uyivumela ukuthi iphumule okungenani ama-5 min. Nciphisa i-supernatant ecacile nenhlanganisela yesikhumba (v / v 1: 20).

I-HPLC-DAD: Amanzi ama-2695 ahlukaniswe modul / 2996 i-PAD injection: 10μL
I-Precolumn: LiChrospher 60, RP-Khetha B, 5μm (Merck 50963)
Ikholomu: LiChroCart 125-4, Lichrospher 60, RP-Khetha B, 5 μm (Merck 50829)
Eluent: isokratic 36% TEAP 25 mmol / L, 64% ye-acetonitrile quantification e-210nm
Ukulinganisa: 0,001 – 0, 10 μg / μL
Ukulinganisa: 0,01 - 0,50 μg / μL
Isikhathi sokugcina: i-CBD: amaminithi angu-6,2; i-CBN: 8,0 min .; I-THC: 9,4 iminithi; I-THC-A: 11,0 iminithi
I-LOD: i-CBD 0,5%, i- CBN 0,5%, i-THC 0,15%, i-THC-A 1%

Cela ulwazi oluthe xaxa

Sicela usebenzise ifomu elingezansi, uma ufisa ukucela ulwazi oluthe xaxa mayelana ne-homogenization yama-ultrasonic. Sizojabula ukukunikeza uhlelo lwe-ultrasonic ukuhlangabezana nezidingo zakho.









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I-ultrasonic disperser yekulungiswa kwe-nano izinhlayiyana zokuchofoza (Chofoza ukuze ukhulise!)

idivayisi ye-ultrasonic lab UP50H



Amaqiniso Okufanele Ukwazi

I-Marijuana liqukethe izimbali ezomile namaqabunga ahlambalazayo neziqu zesitshalo se-Cannabis sesifazane. Leli fomu elidliwe kakhulu futhi liqukethe cishe. 3% kuya ku-20% ye-THC. Okucacile, izinhlobo ezibhekwe kakhulu zensangu zifinyelela okuqukethwe kwe-THC okungaphezu kwama-30%. I-Marijuana ingumthombo wemithombo yonke iminye imikhiqizo ye-THC equkethe umkhiqizo we-cannabis. Ukuguqula i-cannabinoid egcwele kakhulu, i-tetrahydrocannabinolic acid (THCA), ibe yi-THC engokomsebenzi, inqubo ye-decarboylation kumele iqaliswe. Ngakho-ke, i-cannabis kanye neziqhumane zayo kufanele zishiswe noma zichithe amandla emzimbeni ukuze zenze i-decarboxylation.

Kief ibhekisela ku-powder, ecebile nge-trichomes, ehlanjululwa emagqabeni nasezintabeni ze-cannabis. Iqotshwe ngesimo se powder noma icindezelekile futhi isetshenziswe kumaqebelengwane angama-hashish.

Hashishi noma i-hash i-resin ikhekhe noma i-ball ekhishwe yi-kief eshicilelwe noma ephuma ku-resin ekhishwa ebusweni besitshalo futhi ihanjiswe ngamabhola. Iyenzeka ngemibala ehlukahlukene kusuka emnyama kuya enhlabathini ensundu kuye ngokuthi kuhlanzekile nomthombo. Kungadliwa ngomlomo noma ngokubhema, isib. Kuphayiphu, hookah, bong, bubbler, vaporizer, besebenzisa ummese oshisayo, ukubhema ngamalunga, okuxubene namafihleki kagciwane noma ugwayi, ukubhema njengebhokisi lebhokisi noma lisetshenziswe njengesithako ekudleni.

I-Cannabis Tincture ibhekisela ekukhishwe kwamagciwane okuphuza utshwala. Ama-cannabinoids akhishwa esitshalweni se-cannabis besebenzisa imimoya ephezulu (ikakhulukazi okusanhlamvu utshwala) ukudala i-tincture.

Hash amafutha i-matrix ehlaziyekayo ye-cannabinoid eyenziwa yi-solvent extraction, eyakhiwe yaba yinto enzima noma enesisindo. I-hush yamahhashi ingenye yemikhiqizo eningi kakhulu ye-cannabis ngenxa yezinga eliphezulu layo lokuhlanganiswa kwengqondo. Ukusetshenziswa kwamafutha ase-hash okwenziwe i-butane kanye ne-carbon dioxide engaphezu kwesikhashana kuye kwanda ukuthandwa.

I-infusions bhekani ezinhlobonhlobo ze-influence ezingenayo i-cannabis ezihlukile komunye nomunye ngezinhlobonhlobo ezihlukahlukene ze-solvents ezingezona ezithandekayo ezingasetshenziselwa ukukhiqiza ukumnika ukukhishwa kwe-cannabis. Ukulungiselela ukumnika okunjalo, indaba ye-cannabis ixubene ne-solvent bese icindezelwa futhi ihlungiwe ukuhambisa amafutha avela esitshalweni ibe yi-solvent. Ibhotela le-cocoa, ibhotela lebisi, amafutha okupheka, i-glycerine, kanye nama-moisturizers esikhumba ngokuvamile asetshenziselwa izivolumu.

Inyosi yezokwelapha ibhekisela ekusetshenzisweni kwesitshalo se-Cannabis njengendlela yokwelashwa okunconywa odokotela kanye ne-synthetic THC kanye namagciwane. Lokhu ukusetshenziswa ngokuvamile kudinga imithi, futhi ukusatshalaliswa ngokuvamile kwenziwa ngaphakathi kohlaka oluchazwe imithetho yendawo. Ucwaningo oluthile luye lwabonisa ukuthi ukusetshenziswa kwe-cannabis noma okutholakala kwayo kusebenza ngokumelene nesifo sokukhwabanisa esibangwa yi-chemotherapy-ukukhushulwa nokuhlanza, ubuhlungu be-neuropathic, ne-multiple sclerosis.