I-Hielscher Ultrasound Technology

Ukulungiselela i-Ultrasonic ye-Platelet i-Serum ecebile

  • I-plasma ene-platelet ecebile (i-PRP) i-plasma yegazi eye yahanjiswa ngamaplatelets, asetshenziselwa ukwelashwa kwe-medical and estethi.
  • I-ultrasonic lysis kanye nesimo sengqondo kuyindlela ephumelela kakhulu yokulungisa iplasma ecebile epulletheni.
  • I-Hielscher Ultrasonics inikeza izinhlelo ezihlukahlukene ze-ultrasonic ezihambisana nezidingo zokwelashwa kwegazi (isb. Ngaphakathi nangaphandle ukulungiselela ikamelo elihlanzekile).

I-platelet i-Rich Serum

I-Sonication iyindlela enokwethenjelwa futhi elula yokwenza ama-platelets e-autologous blood serum ukuze kukhululwe izici zokukhula. Izingxenyana ezithathwe eplatelet – futhi eyaziwa njenge-autologous conditioned serum (ACS) – asetshenziselwa ukulungiswa kwezicubu noma izifo zokwelapha. Ukuze uthole imiphumela ephumelelayo, i-PRP iyasetshenziswa ngaphambi kokujova.
Ukuze ulungiselele i-plasma ecebile ngezici zokukhula kwe-cell, i-sonication iyindlela ephumelela kakhulu futhi ephumelelayo yokwenza i-membrane yeseli futhi ikhulule cishe izici eziphelele zokukhula kwamaseli avela kuma platelet. Nge-lysis ultrasonic kanye nesimiso, ngisho negazi eliphikisanayo lingasetshenziswa.
Ukubeka isimo se-PRP nge-sonication kuyindlela elula futhi engabizi kahle yokuveza izici zokukhula ezigxile.

Ukulungiselela i-ultrasonic ye-plasma ecebile eplastiki (PRP) © Fortunato, TM et al. (2016)

I-ultrasonic lysis ye-plasma ecebile epulasitiki (i-PRP)

Imiphumela ye-Ultrasonic Lysis

Ukwelashwa kwe-ultrasonic ye-serum yegazi kubangelwa i-lysis ehambisanayo yamaplatelet ukuze kukhululwe izici zokukhula ezingathintekayo (ukukhula kwe-AB (i-PDGF-AB), ukukhula kwesici AA (PDGF-AA), isici sokukhula BB (PDGF-BB), isisulu i-endothelial growth factor (VEGF), iguqula ukukhula kwesici β (TGF-β), i-epidermal growth factor (EGF), i-factor factor growth (IGF), i-epithelial cell factor factor ECGF kanye ne-fibroblastic growth factor (FGF)). Ama-platelets kanye ne-alpha-granules azophazanyiswa yi-sonication, esebenzisa lesi simo i-acoustic cavitation. Ukusebenzisa i-cavitation ye-ultrasonic ye-platelet lysis kunciphisa umthelela omubi ongase ube nawo ekusebenzeni kwezinto zokukhula kwendlela ephindaphindiwe yokuqhafaza.

Isicelo solwazi




Qaphela kwethu Inqubomgomo yobumfihlo.


I-Protocol for Ultrasonic PRP Ukulungiselela

I-probe-type insonifier UP200St ye-lysisOkungenani. I-16mL yesampula yegazi eqoqwe i-autologous ehlelwe ngokucophelela ishintshwe ngokucophelela izikhathi ezingu-8-10 ku-tube ukuze igazi ne-anticoagulants zixutshwe kahle. Ngemuva kwalokho, isampula i-centrifuged ngo-1800 g ngamaminithi angu-20 ekamelweni lokushisa. Ngemuva kwe-centrifugation, ama-platelet namaseli e-mononuclear zakha ungqimba olumhlophe phansi kwesendlalelo se-plasma, eyaziwa ngokuthi i-plasma ecebile eplasma (PRP). Ingqimba engaphezulu iyaziwa njenge-platelet-poor plasma (PPP). Amaseli e-mononuclear angasuswa noma ahlale esampheni. Ngokuvamile, amangqamuzana e-mononuclear ahlala esampula se-PRP ukugwema izinyathelo ezengeziwe, ezithwala ingozi yokungcola.
Qaphela: Ukwelashwa kwe-ultrasonic ye-PRP ye-autologous kufanele kwenziwe ngaphansi kwezimo eziyinyumba.
I-PRP ne-PPP zihlukanisiwe futhi ziqoqwe. Ku-PPP, isixazululo se-calcium chloride se-1.5mL siyatholakala, kanti ku-PRP 0.5mL 5% i-ethanol ingeziwe. Kokubili ukumiswa kushukunyiswa kahle futhi kuvunyelwe ukuma imizuzu engu-5 ekamelweni lokushisa. Okokugcina, i-1mL PPP ingeziwe ekumiswe kwe-PRP futhi ivunyelwe ukuma kuze kube namafayili e-fibrin clot.
Ukwelashwa kwe-ultrasonic: Njengoba i-gel platelet eyakhiwe yenza njengendawo yamanzi okukhululwa, i-gel fibrin kufanele iphazamiseke ukuze ikhulule izici zokukhula ezitholakalayo. Imingcele ye-ultrasonic lysis nokukhululwa kwezinto ezikhulayo yizinto ezilandelayo: Sonicate nge UP200Ht noma UP200St (200W, 26kHz) nge-sonotrode S26d2 isampula e-tube. Ukuze ugweme ukushisa kwesampula segazi, i-sonication ingenziwa emigqeni yokudonsa (isb. 10 sekh., Ku-30 sec.). Isampula kufanele ibekwe eqhweni ukuze kupholile ukuze izinga lokushisa ligcinwe kahle phakathi kwe-2 – 5 ° C. Ngemuva kwe-sonication, isampula i-centrifuged ngo-16000 g ngamaminithi angu-5. ekamelweni lokushisa. I-supernatant iqoqwe futhi ingagcinwa ku -70 ° C.
Ukuhlushwa kweplatelet engxenyeni ye-PRP kwalungiswa 1.7 – 4.5 faka ngendlela ehlonishwayo uma kuqhathaniswa nesilinganiso sezinombolo zamaplatelet esiphezulu egazini lonke.

I-PRP ihlanganiswe ne-adipose-etholakalayo i-stem cell therapy (i-ultrasonically ikhishwe ama-autologous stem cells avela kumcubu we-adipose, ixubene ne-PRP, futhi ijojelwe emuva emathisini omguli wokulungisa).

Izinsiza ze-Ultrasonic

Idivaysi ye-ultrasonic i-VialTweeter ivumela isampuli yokulungiselela okukodwa ngesikhathi esisodwa kuya ngaphansi kwezimo eziyizinqubo ezifanayo. (Chofoza ukuze ukhulise!)I-Hielscher Ultrasonics inguchwepheshe wezobuchwephesha nobuchwepheshe obunokwethenjelwa kwe-ultrasonic. Amadivaysi ethu e-ultrasonic asetshenziselwa umhlaba jikelele ukuze lysis, isisetshenziswa kanye nesampula ukulungiselela – ngokuqondile nangokungaqondile. i-autoclavable kanye ne ilahlwa izesekeli ziqinisekise ukungcola-mahhala ukwelashwa kwe-autologous blood sampuli. Iningi lezinhlelo zethu zingasetshenziswa for lysis ultrasonic of ama-stem cells ne-PRP, okwenza amadivaysi ethu ama-ultrasonic asebenze kangcono nakakhulu nakakhulu.
Leli thebula elingezansi lihlunga amadivayisi wethu we-ultrasonic, afanele ukulungiselela iplasma ecebile eplatelet, kanye ne-volume yesampuli ephakanyisiwe, ngokulandelanayo. Ama-ultrasonicators amakhulu nezinhlelo ezenziwe ngezifiso ziyatholakala, futhi.

Idivayisi Amandla [W] Freq. [kHz] Thayipha Ivolumu [mL]
I-VialTweeter 200 26 ukuma 0.5 1.5
UP50H 50 30 ifakwe ngesandla noma imisiwe 0.01 250
UP100H 100 30 ifakwe ngesandla noma imisiwe 0.01 500
UP200Ht 200 26 ifakwe ngesandla noma imisiwe 0.1 1000
UP200St 200 26 imisiwe 0.1 1000
UP400St 400 24 imisiwe 5.0 2000
UP200St-SonoStep 200 26 ukuma 30 500
I-GDmini2 200 26 ukungcoliswa-free flow cell

Sicela uxhumane nathi namuhla! Sizojabula ukukusiza ukuthola idivayisi enhle kakhulu ye-ultrasonic!

Xhumana nathi! / Cela Us!

Cela ulwazi oluthe xaxa

Sicela usebenzise ifomu elingezansi, uma ufisa ukucela ulwazi oluthe xaxa mayelana ne-homogenization yama-ultrasonic. Sizojabula ukukunikeza uhlelo lwe-ultrasonic ukuhlangabezana nezidingo zakho.









Sicela uqaphele wethu Inqubomgomo yobumfihlo.


I-GDmini2 ku-UP200St-TD Transducer (ama-watts angu-200) we-sonication engenawo ukungcola

I-GDmini2 ku-UP200St-TD Transducer (ama-watts angu-200)

Izincwadi / Izinkomba

  • I-Fortunato, TM; I-Beltrami, i-Ch .; U-Emanueli, C .; De Bank, PA :; I-Pula, G. (2016): i-platelet i-lysate gel kanye nama-progenitors endothelial akhuthaza ukubunjwa kwenethiwekhi ye-microvascular in vitro: izinyathelo zobunjiniyela zobuningi. Sci. Umhlaka 6, 2016.
  • I-Hamid, i-MSA; U-Yusof, u-Ashril; U-Ali, Mohamed Razif Mohamed (2014): I-Platelet-Rich Plasma (PRP) ye-Acute Muscle Injury: Ukubuyekezwa Okuhlelekile. I-PLOS ONE Volume 9, Issue 2, 2014.


Amaqiniso Okufanele Ukwazi

I-Platelet Rich Plasma

I-plasma eline-Platelet ecebile iqukethe i-factor factor AB (i-PDGF-AB), i-platelet etholakalayo ukukhula kwe-AA (PDGF-AA), i-platelet etholakalayo ukukhula kwe-BB (i-PDGF-BB), isisombululo sokukhula se-platefelial (VEGF), okuguqula ukukhula kwe-β (TGF) -β), i-epidermal growth factor (EGF), i-insulin-like growth factor (i-IGF), i-epithelial cell factor factor ECGF kanye ne-fibroblastic growth factor (FGF), okuyizinto ezibalulekile zokukhuthaza ukukhula kwamaseli nokuvuselela.

Isimo se-Autologous Conditioned (ACS)

I-Plasma ebizwa ngokuthi i-Autologous Conditioned Plasma (ACP) iyi-plasma ecebile eplatelet ekhishwa egazini elizenzekelayo elisetshenziselwa ukusekela ukuvuselelwa ngezimo ezihlukahlukene zamazinyo nezinqubo zokuhlinza. I-serum enesimo ilungiselelwa ngokuhlukanisa i-plasma kusuka kwezinye izingxenye zegazi (njenge-erythrocytes), futhi uyigxilise. I-ACP iyi-plasma ecebile eplasma (PRP). I-PRP yigama elijwayelekile lomhlobo we-plasma equkethe ukuhlushwa okukhulu kwe-thrombocytes uma kuqhathaniswa negazi lonke. Ikhishwa egazini lonke. Izingxenye eziyinhloko ze-ACP zihlanganisa i-thrombocytes (amaplatelets), kanye nezici eziningana zokukhula ezidlala indima ebalulekile ekuphuculeni. Ngokungafani nezinye izinhlobo ze-plasma ezicebile zeplatelet, i-ACP ihlukaniswa ukuhlushwa okuphansi kwamangqamuzana egazi amhlophe njengama-neutrophil granulocytes angaphazamisa inqubo yokuphulukisa lapho ekhona ekugxilweni okuphezulu.

Ama-injection e-Platelet-Rich Rich Plasma

I-plasma elineplatelet ecebile isetshenziswa njengomjovo ekudleni okuvuselelayo kanye nasezinkambisweni zezimonyo. Ukusetshenziswa kwe-PRP kuthembisa ukusheshiswa okuphawulekayo kokuphulukiswa kwamasuli nokuthola imisipha. Ngaphezu kwalokho, isetshenziswa ngokuphumelelayo ukuphatha ukuvuvukala, isifo samathambo, ubuhlungu, amanxeba, amanxeba okuphulukisa okuphuthumayo, nokushisa okukhulu kwesikhumba. I-PRP iqukethe izinga eliphakeme lokukhula, okugqugquzela nokuvikela ama-a-cell awo okuphulukisa ama-tissu abonakalisiwe alungiswa.
Ukuze uthole izidakamizwa zezimonyo, i-PRP iboniswe ngempumelelo ukuze kukhuthazwe izinwele, ukuvuselelwa kwesikhumba (eyaziwa nangokuthi ukwelashwa kwe-vampire).

Izinto ezikhungethwe yi-Platelet-Derived Growth (PDGF)

Igama le-platelet-derived growth factor (PDGF) lisho izici PDGF-A, PDGF-B, PDGF-C kanye ne-PDGF-D. Kuyinto inethiwekhi yokubonisa imigqa emine (PDGF-A, PDGF-B, PDGF-C kanye ne-PDGF-D), nama-receptors amabili, i-PDGFR-alpha ne-PDGFR-beta.
Izici zokukhula, noma amaprotheni, zilawula ukukhula kwamaseli nokuhlukaniswa. Njengoba i-mitogen eyinhloko etholakalayo kuma-mammalian serum, ekhishwa kuma-plates ngesikhathi sokubunjwa kwe-clot, idlala indima ebalulekile ekwakheni izitsha zegazi (i-angiogenesis), ukukhula kwemithambo yegazi kusuka emathanjeni omzimba aselevele ekhona.