I-Hielscher Ultrasound Technology

Ultrasonic Liposome Ukulungiselela

Ultrasonic Liposome Ukulungiselela Imithi Nezimonyo

I-liposomes (vesposic-liposome-lipid based), i-transfosomes (i-liposomes ultradeformable), i-ethosomes (i-vesicles ene-ultradeformable enohlobo oludakayo lokuphuza utshwala), kanye nama-niosomes (ama-synthetic vesicles) angama-vesicles amancane, angahle alungiselelwe njengama-globular abathwali lapho i-molecule esebenzayo ingafakwa khona . Lezi zingubo ezinama diameter eziphakathi kuka-25 no-5000 nm zivame ukusetshenziswa njengabathwala izidakamizwa ngezinhloso zezokwelapha embonini yezokwelapha nezimonyo, ezifana nokulethwa kwezidakamizwa, i-genetherapy, nokugoma. I-Ultrasound iyindlela eqinisekisiwe yokulungiswa kwe-liposome kanye ne-encapsulation yama-agent asebenzayo kulezi zingubo.

Liposomes are made from Phosphatidyl Choline (PC)

I-Liposomes ayiyona nje imithwalo yezinhlaka ezisebenzayo, futhi ngaphandle kwama-agent ahlanganisiwe, ama-vesicles angenayo, asebenza ngokuqinile esikhumbeni, njengoba i-phosphatidylcholin ihlanganisa izinto ezimbili ezibalulekile, okungezona umzimba ongenakuzikhiqiza ngokwazo: i-linoleic acid ne-choline.

Liposomes

I-Liposomes yi-unilamellar, i-oligolamellar noma i-multilamellar systems vesicular futhi iqukethe into efanayo ne-membrane yeseli (lipid bilayer). Ngokuphathelene nokubunjwa nobukhulu bawo, ihluke phakathi kwama-multi-lamellar vesicles (i-MLV, i-0.1-10μm) nama-vesicle angama-unilamellar, ahlukaniswa phakathi kwamancane (i-SUV, <100 nm), enkulu (LUV, 100-500 nm) noma i-giant (i-GUV, ≥1 μm) ama-vesicle.
Isakhiwo esakhiwa ngama-liposomes sine-phospholipids. I-Phospholipids ineqembu eliyinhloko le-hydrophilic kanye neqembu le-tail hydrophobic, elinekethala elide le-hydrocarbon.
I-membrane ye-liposome inomumo ofanayo kakhulu njengesikhawulo sesikhumba, ukuze ihlanganiswe kalula esikhunjeni somuntu. Njengoba i-liposomes ihlangana ngesikhumba, ingakwazi ukulayisha izithunywa eziboshwe ngokuqondile endaweni lapho khona, lapho izisebenzi zikwazi ukufeza imisebenzi yazo. Ngakho-ke, i-liposomes idala ukuqiniswa kwengqondo / ukukhishwa kwezimpawu zamakhemikhali nezimonyo eziboshwe. Futhi ama-liposomes ngaphandle kwama-agent ahlanganisiwe, ama-vesicles angenayo, asebenza ngokuqinile esikhumbeni, njengoba i-phosphatidylcholin ihlanganisa izinto ezimbili ezibalulekile, okungeyona into eyenziwa ngumuntu engakwazi ukukhiqiza yona ngokwayo: i-linoleic acid ne-choline.
Ama-liposomes asetshenziswa njengezinsimbi eziphathelene nezidakamizwa, ama-peptide, amaprotheni, i-DNA ye-plasmic, i-oligonucleotides noma i-ribozymes, i-anti -ense oligonucleotides noma i-ribozymes, ngenhloso yokuthola imithi, izimonyo, kanye ne-biochemical. Ubukhulu obuningi obukhulu obuncane bezinhlayiyana kanye nemingcele yemvelo ye lipids inika amandla okukhangisa ekwakheni izimoto ezenziwe ngokwezifiso ezinhlobonhlobo zezicelo. (Ulrich 2002)

I-Ultrasonic Liposomes Formation

I-liposomes ingenziwa ngokusetshenziswa kwe-ultrasonics. Izinto eziyisisekelo zokwabiwa kwe-liposome yizimvukuzane ze-amphilic ezithathwe noma ezisekelwe ku-membrane lipids. Ukuze kwakhiwe ama-vesicle amancane angaphansi kwe-unilamellar (SUV), ukusabalalisa kwe-lipid kuhanjiswa ngomusa – isib nge-ultrasonic device ephathekayo UP50H (50W, 30kHz), i I-VialTweeter noma i-reactor ultrasonic UTR200 – ebhodini lokugeza. Ubude bendlela yokwelapha olunjalo lwe-ultrasonic luhlala ngo-approx. Imizuzu engu-5 kuya kwemi-15. Enye indlela yokukhiqiza ama-vesicles angama-unilamellar amancane yi-sonication we-multi-lamellar vesicles liposomes.
UDinu-Pirvu et al. (2010) ubika ukutholakala kwama-transfosomes nge-Sonicating MLVs ekamelweni lokushisa.
I-Hielscher Ultrasonics inikeza amadivaysi ahlukahlukene e-ultrasonic, i-sonotrodes nezesekeli ukuhlinzeka nge-sonicator efanele ngokuphathelene namandla

I-ultrasonic encapsulation yama-agent ku-liposomes

I-Liposomes isebenza njengabathwali bama agent asebenzayo. I-Ultrasound iyithuluzi eliphumelelayo lokulungiselela nokwakha i-liposomes yokufaka ama-agent asebenzayo. Ngaphambi kokufakwa kwe-encapsulation, i-liposomes ijwayele ukudala amaqoqo ngenxa yesikhala sokukhokha-ukusebenzisana kwenkokhelo yamakhanda e-phospholipid polar (Míckova et al. 2008), futhi kufanele avuleke. Ngomzekelo, uZhu et al. (2003) uchaza ukufakwa kwe-biotin powder kuma-liposomes nge-ultrasonication. Njengoba i-biotin powder yanezelwa kwisisombululo sokumiswa kwe-vesicle, isixazululo sithunyelwe nge-approx. Ihora eli-1. Ngemva kwalokhu ukwelashwa, i-biotin yayibanjwe yi-liposomes.

High power ultrasonicators from Hielscher Ultrasonics enable for targeted liposome preparation, emulsification and dispersing.

Khetha. 1: 1kW iprosesa ye-ultrasonic for processing okuqhubekayo inline

Liposomal Emulsions

Ukuze kuthuthukiswe umphumela wokondla we-cremes noma anti-aging cremes, ama-lotions, ama-gel kanye nezinye izinhlobo zokwenziwa kwe-cosmeceutical, i-emulsifier yengezwe kuma-liperomal dispersions ukuze kuqinisekiswe inani eliphakeme le-lipids. Kodwa uphenyo luye lwabonisa ukuthi ikhono lama-liposomes ngokuvamile alinganiselwe. Ngokungeziwe kwama-emulsifiers, lo mphumela uzovela ngaphambi kwesikhathi futhi ama-emulsifiers angeziwe abangela ukwehlisa amandla okubambisana kwe-phosphatidylcholine. Nanoparticles – eyakhiwe i-phosphatidylcholine ne-lipids - iyimpendulo yale nkinga. Lezi nanoparticles zakhiwa yi-droplet ye-oyile ehlanganiswa umxhwele we-phosphatidylcholine. Ukusetshenziswa kwe-nanoparticles kuvumela ukuhlanganiswa okukwazi ukuzuza amap lipids amaningi futhi uhlale uzinzile, ukuze amanye ama-emulsifiers angadingeki.
Ultrasonication iyindlela eqinisekisiwe yokukhiqizwa Ama-nanoemulsions futhi nanodispersions. I-ultrasound enamandla kakhulu inikeza amandla adingekayo ukusabalalisa isigaba samanzi (isigaba esasakazekile) ngamaconsi amancane esigabeni sesibili (isigaba esiqhubekayo). Endaweni yokuhlakazeka, ukungena cavitation ama-bubbles adala amagagasi okhathazeka kakhulu emanzini azungeze futhi akhiqize ukwakheka kwamanzi ahlanzekile e-liquid velocity. Ukuze kuqiniswe ama-droplets asanda kuhlanganiswa kwesigaba sokuhlakazeka ngokumelene ne-coalescence, ama-emulsifiers (izinto ezibonakalayo ezibonakalayo, ama-surfactants) kanye nokuqiniswa okungeziwe ku-emulsion. Njengoba u-coalescence wamaconsi ngemuva kokuphazanyiswa kuthinta ukusatshalaliswa kokusatshalaliswa kokusakaza kwe-droplet, kusetshenziselwa ukusimama kokugcina kwe-droplet esezingeni elilingana nokusatshalaliswa ngokushesha ngemuva kokuphazanyiswa kwe-droplet endaweni ye-dispersing ultrasonic.

Liposomal Dispersions

Ama-liposomal dispersions, asekelwe ku-phosphatidylchlorine engaxhunyiwe, engenakho ukuzinza ngokumelene ne-oxidation. Ukuqiniswa kokusabalalisa kungafinyelelwa ngama-antioxidants, njenge-complex of vitamin C no-E.
U-Ortan et al. (2002) bazuze esifundweni sabo mayelana nokulungiswa kwe-ultrasonic ka-Anethum graveolens amafutha abalulekile kuma-liposomes imiphumela emihle. Ngemuva kokubeletha, ubukhulu bama-liposomes babuphakathi kuka-70-150 nm, kanye ne-MLV phakathi kuka-230-475 nm; lezi zimiso zazihlala njalo njalo emva kwezinyanga ezimbili, kodwa zingakapheli inyanga engu-12, ikakhulukazi ekusakazeni kwe-SUV (bheka i-histograms ngezansi). Ukulinganiswa kokuzinza, ngokuphathelene nokulahlekelwa kwamafutha okubalulekile nokusabalalisa usayizi, kubonise nokuthi ama-liposomal dispersions agcina okuqukethwe kwamafutha ashubile. Lokhu kusikisela ukuthi ukufakwa kwamafutha ebalulekile kuma-liposomes kwandise ukuzinza kwamafutha.

Long-time stability of ultrasonically prepared multilamellar (MLV) and small unilamellar (SUV) vesicle dispersion.

I-Fig.1 + 2: Ortan et al. (2009): Ukuzinza kwe-MLV ne-SUV ukuhlakazeka ngemva konyaka owodwa. Ukubunjwa kwe-Liposomal kwagcinwa ku-4 ± 1 ºC.

Amaprosesa we-Hielscher ultrasonic yiwona amadivayisi afanelekayo wezinhlelo zokusebenza izimonyo kanye nomkhakha wezokwelapha. Izinhlelo eziqukethe amaprosesa amaningana e-ultrasonic Ama-watts angu-16,000 ngayinye, unike amandla okudingekayo ukuhumusha lolu hlelo lokusebenza lwelebhu ngendlela ephumelelayo yokukhiqiza ukuze uthole ukuthuthwa kwe-emulsions ehlelekile ekugezeni okuqhubekayo noma ku-batch – ukufezekisa imiphumela efana ne-homogenizers ephakeme kakhulu ephezulu yanamuhla, efana ne-valve entsha ye-orifice. Ngaphezu kwalokhu kusebenza kahle okuqhubekayo emulsification, Amadivaysi e-Hielscher ultrasonic adinga ukunakekelwa okuphansi kakhulu futhi kulula kakhulu ukusebenza nokuhlanza. I-ultrasound iqinisile isekela ukuhlanza nokuhlanza. Amandla we-ultrasonic ayaguquguquka futhi angashintshwa kumikhiqizo ethile kanye nezidingo ze-emulsification. Izigameko ezikhethekile ze-flow cell (cell-reactors) zihlangana ne-CIP ethuthukile (ihlanzekile-indawo) kanye nezidingo ze-SIP (inzalo-in-place) nazo ziyatholakala.

Xhumana Nathi / Cela Ukwaziswa Olwengeziwe

Khuluma nathi mayelana nezidingo zakho zokucubungula. Sizosikisela ukusetha nokulungiswa kwemingcele efanele yephrojekthi yakho.





Sicela uqaphele wethu Inqubomgomo yobumfihlo.


Izincwadi / Izinkomba

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