I-Hielscher Ultrasound Technology

Extraction Ultrasonic of Spirulina Pigments

Ukukhishwa kwe-Ultrasonic kufakazelwe ukuthi kusebenza kahle kakhulu ekuhlukaniselweni kokukhishwa kwekhwalithi ephezulu ye-microalgae. Izimbali ze-Spirulina ezifana ne-phycocyanin zaziwa njenge-superfood, ezinomthelela kwizinzuzo zezempilo ngenxa yokuqukethwe kwazo okuphezulu kwama-antioxidants, amavithamini namaprotheni. Ukuze ukhiqize izithasiselo ezigxile kakhulu ezinjengo-powders kanye namathebulethi, imibala eluhlaza okwesibhakabhaka kumele ikhishwe ku-spirulina algae.

I-Spirulina

I-Arthrospira platensis (A. platensis) ne-Arthrospira maxima (A. maxima) ziyaziwa ngaphansi kwegama elithi spirulina. ISpirulina iyi-algae eluhlaza okotshani obuluhlaza, enothile ngama-amino acid kanye nama-antioxidants. Ngomthwalo omningi wezakhi ezinjengamaprotheni, ama-enzyme, amaminerali, namavithamini A, K, B12, i-iron ne-manganese, ahlukaniswe njenge-superfood. I-algae genus Arthrospira inokuqukethwe okuphakeme kakhulu kwamaprotheni. Okuqukethwe ngamaprotheni kusuka ku-53 kuya ku-68% ngesisindo esomile futhi kunikeza inani eliphelele lawo wonke ama-amino acid abalulekile. Ngaphezu kwalokho i-Arthrospira inamanani amaningi ama-polyunsaturated fatty acids (PUFA), okuyi-approx. I-1.5 kuye ku-2% yokuqukethwe okuphelele kwe-lipid ka-5 kuye ku-6%. Lama-PUFA aqukethe i-γ-Linolenic acid (GLA), i-Omega-6 Fatty acid ebalulekile. Ezinye izinto ezihlanganiswa yi-Arthrospira zihlanganisa amavithamini, amaminerali kanye nemibala ethambile.
Qaphela: Ngokwesayensi, uSpirulina platensis (S. platensis) noSpirulina maxima (S. maxima) babizwa ngokufanele ngokuthi i-Arthrospira platensis (A. platensis) ne-Arthrospira maxima (A. maxima). Zombili lezi zinhlobo zazikade zihlukaniswa kuhlobo lwe-Spirulina futhi kuze kube namuhla zaziwa kakhulu ngegama le-spirulina. Yize sekwethulwa i-genera ehlukene ye-Arthrospira ne-Spirulina manje sekuyamukelwa ngokuvamile, igama elithi spirulina livame ukusetshenziswa njengegama lesambulela.

Ultrasonic Spirulina Extraction

Ama-ultrasonic cell disruptors (aphinde abizwa ngokuthi ama-ultrasonic ultrasonic) aziwa ngokukwazi kwabo ukuvula amaseli ukuze akhulule impahla ye-intracellular. Lapho amagagasi amakhulu e-ultrasound ehlanganiswa abe ngamanzi, i-cavitation ye-acoustic ivela. Amandla okucabangela ukuphazamisa izindonga zezingcingo futhi ethuthukisa ukudluliselwa kwamandla ukuze izinhlanganisela ezibhekiswe kuzo zihanjiswe kusuka esitokisini zibe yiketshezi.

Iziphazamisi ze-Ultrasonic zisetshenziselwa ukuchithwa okuvela emithonjeni ye-phyto (isb. Izitshalo, i-algae, isikhunta)

Isizinda se-ultrasonic esivela kumaseli: isigaba esincane esithintekayo (TS) sibonisa indlela yezenzo phakathi kwesizinda se-ultrasonic kusuka kumaseli (ukukhulisa 2000x) [isisetshenziswa: Vilkhu et al. 2011]

Isicelo solwazi




Qaphela kwethu Inqubomgomo yobumfihlo.



Izinzuzo ze-Ultrasonic Extraction

  • isivuno high
  • Ikhwalithi Yokukhishwa Okuphezulu
  • Ukukhipha okusheshayo
  • Inqubo Ebukhali, Engekho Eyashisayo
  • i-solvents ehlukahlukene
  • ephephile
  • Lula ukusebenza
  • ukulinganisa okulinganayo
  • Ukugcinwa okuphansi
  • i-RoI esheshayo

Uhlelo Lokungena

Prabuthas et al. (2011) asungule le protocol yokukhishwa elandelayo ukuze kutholakale ikhwalithi ephezulu ye-phycocyanin ekhishwe: Ukukhishwa kwe-Phycocyanin kwahlolwa ngokuya nge-phycocyanin okuhlushwa kusetshenziswa amanzi ahlanekezelwe nesixazululo se-1% seCaCl2. I-algae spirulina isetshenziswa ngendlela eyomile ngoba i-biomass emanzi isetshenziswa ngokushesha ngamagciwane futhi iqala ukonakala ngenxa yokwakheka kwayo okunomsoco. Ngakho-ke ukugwema lezi zinkinga, kunconywa ukusetshenziswa kwe-algae biomass eyomile. Ukukhishwa kwenziwa ngokuxuba i-0,1g ye-biomass eyomisiwe nenani le-solvent, i-sonication yenziwa isikhathi kanye ne-amplitude ngokusho komklamo wokuhlola. Ukumiswa kwesampula kwenziwa ngokuthi kusetshenziswe ukuphazanyiswa kweseli kwe-Hielscher's UP50H, kwalandelwa ukukhipha imizuzu eyi-15 ngehora le-13500rpm kusetshenziswa isiqandisi sefriji. I-phycocyanin kanye nokuqukethwe kwamaprotheni ngesikhathi sokukhishwa ngezinsimbi ezihlukile kwakuyicalcium chloride kanye namanzi aphalalisiwe aphenywayo.
Isamba esiphezulu se-phycocyanin, i-0.3116mg / ml satholakala kwisisombululo se-calcium chloride salandelwa ngu-0,099mg / ml emanzini afakwe emanzini. Inani eliphakeme lamaprotheni, ama-63.63% atholakala ku-solvent yamanzi e-solvent no-54.69% kusisombululo se-calcium chloride. Ngakho-ke isixazululo seCaCl2 satholakala kokuxazululwa okungcono kakhulu kokukhishwa kwe-phycocyanin ngosizo lwe-ultrasound.

Ukusebenza okuphezulu kwe-Ultrasonic Extractors kwe-Spirulina Khipha

I-Hielscher ngumlingani wakho wesikhathi eside onolwazi uma kuziwa ekususeni okunamandla, okuthembekile kwe-ultrasonic yama-biioass kusuka ezitshalweni naku-phyto biomass. Zonke izingcwecwe ze-ultrasonic zingaqhutshwa ngo-24/7 futhi zilungele ukukhiqizwa okuthengiswayo kokukhishwa kokudla. Uhla lomkhiqizo wethu luhlanganisa ukubukwa okugcwele kusuka kuma-biodisruptors amancane aphethwe ngesandla kuya kuma-ultrasonicators endiza nezinhlelo ze-ultrasonic zezimboni. Izesekeli ezahlukahlukene ezinjengama-sonotrodes ahlukene kanye nama-flow cell reactors avumela ukuguqulwa okuhle kwezidingo zenqubo yakho. Zonke izinqubo zokusebenzisa ze-Hielscher ze-Hielscher ziqinile, kulula ukuzisebenzisa futhi zenzelwe ubungani bomsebenzisi. Ama-ultrasonicators ethu alawulwa wedijithali eza nekhadi le-SD elakhelwe ngaphakathi lokuzivumelanisa ngokuzenzakalelayo nedatha ye-sonication.
Ithebula elingezansi linikeza isibonakaliso somthamo wokucubungula we-ultrasonicators wethu:

Iqoqwana Ivolumu flow Rate Amadivayisi ezinconyiwe
1 kuya ku-500mL 10 kuya ku-200mL / min UP100H
10 kuya ku-2000mL 20 kuya ku-400mL / min UP200Ht, UP400St
0.1 kuya ku-20L 0.2 kuya ku-4L / min I-UIP2000hdT
10 kuya ku-100L 2 kuya ku-10L / iminithi UIP4000
na 10 kuya ku-100L / iminithi UIP16000
na sikhulu yinhlanganisela UIP16000
Kungani i-Hielscher Ultrasonics?

  • Okuhlangenwe nakho kwesikhathi eside ku-sonication
  • Ikhwalithi ephezulu
  • ukuqina
  • Ukusebenza komhlaka 24/7
  • Ukuvikelwa okumanzi kuvikelwe
  • ukusabalalisa okulinganayo
  • Isevisi yamakhasimende enhle kakhulu
  • Ukugcinwa okuphansi
  • ukuqeqeshwa & isevisi yokufaka
  • Isikhungo sobuchwepheshe
  • Inqubo Development
  • Imishini ye-ultrasonic eyenziwe ngokwezifiso

Abasebenzi bethu abaqeqeshwe kahle baqinisekisa ukuhlinzekwa kwamakhasimende aphakeme kanye nokubonisana nokuqeqeshelwa kwezobuchwepheshe.
Thintana nathi namuhla! Siyajabula ukukuncoma udokotela osebenza kakhulu we-ultrasonic wesicelo sakho.

Cela ulwazi oluthe xaxa

Sicela usebenzise ifomu elingezansi, uma ufisa ukucela ulwazi oluthe xaxa mayelana ne-homogenization yama-ultrasonic. Sizojabula ukukunikeza uhlelo lwe-ultrasonic ukuhlangabezana nezidingo zakho.









Sicela uqaphele wethu Inqubomgomo yobumfihlo.


I-Hielscher Ultrasonics ikhiqiza ama-ultrasonicators asebenzayo ngokugcwele okuvela ku-spectrum.

I-ultra-power ultrasonic extractors kusukela ebhayisini kuya ku-pilot kanye nesilinganiso sezimboni.

Izincwadi / Izinkomba

  • Bachchhav MB, Kulkarni MV, Ingale AG (2017): Isizinda esiphumelelayo se-phycocyanin nge-ultrasonication nokuhlukaniswa usebenzisa 'ukushukela ngaphandle'. I-Phykos 47 (2): 19-24 (2017).
  • Prabuthas P., Majumdar S., Srivastav PP, Mishra HN (2011): Ukumiswa kwendlela esheshayo neyokonga ye-neutraceuticals extraction kusuka ku-algae. Umagazini weMikhiqizo egcinwe kanye noPhando lokuPhumela emva kokuvuna Vol. 2 (5) k. 93 – 96, Meyi 2011.


Amaqiniso Okufanele Ukwazi

I-Spirulina

ISpirulina iluhlobo lwe-cyanobacteria (eyaziwa nangokuthi i-cyanophyta) okuyigilamu yamagciwane athola amandla awo ngokusebenzisa i-photosynthesis. Yiwo kuphela ama-prokaryotes wezithombe okwazi ukukhiqiza umoya-mpilo. Igama elithi cyanobacteria livela kumbala wamagciwane (ngesiGreek: κυανός, translate. Kyanós, lit. blue). Ngenxa yombala wabo babizwa nangokuthi i-algae eluhlaza okotshani, yize igama elithi i-algae ekusetshenzisweni kwanamuhla livunyelwe kuma-eukaryotes. I-Spirulina platensis (S. platensis) yi-multifilamentous prokaryotic cyanobacterium engakhula kalula njenge-monoculture emachibini avulekile noma kuma-bioreactors avaliwe. I-C-phycocyanin (C-PC) yiyona phycobiliprotein enkulu ese-spirulina.

Ama-extracts e-Spirulina

I-Spirulina iyisithako esidla ukudla esidumile esivela ku-biomass ye-cyanobacteria yasolwandle (i-blue-green algae). Izinhlobo ezimbili ze-spirulina ezisetshenziselwa ukulungiselela amaprotheni we-vegan kanye nama-pigment supplement yi-Arthrospira platensis ne-Arthrospira maxima. Ukukhishwa okukhishwe kulezi zinkampani ezincane kucebile kuma-antioxidants, ama-amino acids namavithamini. Ngakho-ke, i-powder yocwecwe eluhlaza okwesibhakabhaka iyongezwa kumajusi, i-smoothies, i-shakes and iziphuzo futhi ibanika umbala omuhle okwesibhakabhaka. Ngaphandle kwalokho, ukukhishwa kwe-spirulina kungadliwa ngesimo sethebhulethi.
I-Phycocyanin allophycocyanin ne-phycoerythrin ziyi-pigment-protein complexes ezivela emndenini wokuvuna ukuvuna i-phycobiliprotein, eyaziwa ngombala wabo obuluhlaza obuluhlaza. I-Phycocyanin iyi-pigmentary accessory ku-chlorophyll. Njengoba zonke izinhlobo ze-phycobiliprotein zingencibilika kwamanzi, azikho ngaphakathi kwendwangu, esikhundleni salokhu i-phycobiliprotein iboshwe njengama-aggregates (okuthiwa i-phycobilisomes) esithombeni segesi.

Isizinda se-Ultrasonic kanye nokuphazamiseka kweseli

I-Ultrasound-Assisted Extraction (UAE) isetshenziselwa ukukhulula futhi ihlukanise ama-bioactive compounds kusuka ku-phyto impahla kanye nezicubu.
Ngezinqubo zokukhipha isizinda se-ultrasonic, amagagasi amakhulu e-ultrasound ahlanganiswa abe yisilinganiso lapho amagagasi efaka khona ukuguquguquka nokwanda. Ngesikhathi semijikelezo yokucindezelwa / yokwanda, kudalwa amabhuleki we-vacuum akhula ngaphezu kwemijikelezo eminingana aze angakwazi ukubamba amandla amaningi ukuze afaka ngobudlova. I-bubble implosion ikhiqiza isimo esinamandla kakhulu namazinga okushisa endawo afinyelela lapho afinyelela ku-5000K, umehluko wengcindezi we-1000atm, ukushisa kanye namazinga wokupholisa ngenhla kwe-1010 K / s kanye namajethi awuketshezi anezikhala ezingama-280m / s. Le nto yaziwa ngokuthi i-acoustic cavitation.

I-ultrasonic / acoustic cavitation idala amabutho amakhulu kakhulu avula izindonga zeseli eziziwa ngokuthi i-lysis (Chofoza ukuze ukhulise!)

Isizinda se-ultrasonic sisekelwe ku-cavitation yama-acoustic namandla ayo we-hydrodynamic shear