I-Hielscher Ultrasound Technology

Ultrasonic Isizinda of yeselula Matter

Isizinda se-ultrasonic noma i-sono-isizinda inqubo yokwandisa ubuchwepheshe, esebenza ngokuhlanganiswa kwamandla aphakeme e-ultrasonics abe yi-slurry yezitshalo noma izicubu zesisindo. Hielscher Ultrasonics inikeza ultrasonicators ezinokwethenjelwa for cell ukuphazamiseka kanye isizinda kusuka ezincane amasampula webhu kuze kube nemibandela ephezulu ku ukuqhutshwa kwezimboni. Isikhalazo se-ultrasonically isizinda sesisizo sisekwelapha okungasetshenzisiwe kwezinto ezibonakalayo, uhlelo lwayo olulula nokulinganisa kusuka ekuhlolweni kuya esikalini sokukhiqiza.
Amadivaysi e-ultrasonic aseHielscher enza i-ultrasound ephakeme kakhulu engakwazi ukulawulwa ngokufanele ezidingo zakho zenqubo.

Uhlu olungezansi lunikeza izinqubo ezihlukahlukene zokucubungula i-ultrasonic, ezibonisa insimu ebanzi yesicelo samandla ultrasonics ku-biology, ukuphathwa kokudla nokukhiqiza imithi.
I-homogenizers ye-ultrasonic ngamathuluzi anamandla wesithamo. (Chofoza ukuze ukhulise!)

I-Probe-type ultrasonicator UP50H

Ukukhishwa kwe-ultrasonic ye-Chilli Flakes

I-Boldine evela eBoldo ishiya (U-Peumus boldus Molina)

Isicelo se-Ultrasonic:
I-composite ebalulekile esebenzayo ku-boldo yi-boldine ((S) -2,9-dihydroxy-1,10-dimethoxiaporphine), ngaphandle kwe-catechin ((2S, 3R) -2- (3,4-dihydroxy-phenyl) -3 , I-4-dihydro-1 (2H) -benzopyran-3,5,7-triol) izingxenye eziyinhloko zengxenyana ye-alkaloid ne-flavonoid emaqabunga e-boldo. I-Boldine i-agent enamandla e-antioxidant eyenza umonakalo ongenawo ama-peroxidative mahhala futhi usebenza njenge-scavenger eyi-hydroxyl esebenzayo.
Inqubo Yokususa: Ngenqubo evamile yokufakelwa, amasampula amaqabunga e-boldo akhishwa ngamanzi angu-1L emanzini angcolile ngomsindo womoya esebenzisa i-ultrasonicator I-UIP1000hd ku-batch ne-flow-through mode. Isikhathi samakamelo okukhipha phakathi kwamaminithi angu-10 no-40., Nge-Ultrasonic ngamandla we-10 kuya ku-23 W / cm2, kanye nobubanzi bokushisa obuphakathi kuka-10 kuya ku-70 ° C. Imiphumela emihle kakhulu iphumelele ngaphansi kwezimo ezilandelayo: ukuqina kwe-ultrasonic ka-23 W / cm2 iminyaka engu-40. ekushiseni kuka-36 ° C
Imiphumela: Imiphumela yokuhlaziya ibonisa ukuthi ukukhuliswa kwamandla amakhulu kukhulisa ukukhishwa kwe-analyte yempahla yemifino yaseBoldo ngamazinga angcono kakhulu uma kuqhathaniswa nendlela evamile: isivuno esifanayo sakhishwa ngo-sonication ngamaminithi angu-30. ngenkathi kuvamile isikhathi sokukhipha kwaba ngu-2h.
I-Chemat (2013) kanye nabasebenzi abambisene nabo baye babonisa ukuthi isizinda esisizwa nge-ultrasonically sithuthukisa ukusebenza kahle kwesitshalo sesitshalo ngenkathi kuncishiswe isikhathi sokukhipha ngesikhathi sokukhushulwa kweziqhumane (isamba esifanayo solvent and plant material). Ukuhlaziywa kwembula ukuthi izimo ezilungiselelwe kwakuyizi: amandla we-sonication 23 W / cm2 nge I-UIP1000hd iminyaka engu-40. kanye nokushisa kwama-36 ° C. Imiphakamiso ehlelwe yesikhumba se-ultrasound inikeza isizinda esingcono uma siqhathaniswa ne-maceration ejwayelekile ngokwemigomo yesikhathi senqubo (30 min esikhundleni semaminithi angu-120), isivuno esiphezulu, ukusebenza kahle kwamandla, ukuhlanzeka okuthuthukile, ukuphepha okuphakeme kanye nemfanelo yomkhiqizo ongcono.
Incomo yedivayisi:
I-UIP1000hd nge-sonotrode BS2d34 nokugeleza kweseli
Isikhombo / Iphepha Lokucwaninga:
I-Petigny, L .; I-Périno-Issartier, iS .; Wajsman, J .; Chemat, F. (2013): Isisetshenziswa Sokubambisana Ne-Continuous Ultrasound Esenziwe Nge-Boldo Leaves (Peumus boldus Mol.). I-International Journal ye-Molecular Science 14, 2013. 5750-5764.

I-chlorogenic acid kusuka emaqabunga ogwayi

Isicelo se-Ultrasonic:
Ngokuhlolwa kokukhishwa, amaqabunga kagwayi avela eNicotiana tabacum ahlanganisiwe kumasayizi amancane amancane ezinhlayiya cishe >4 x ≧ 2 mm. Ukuze kwenziwe izilingo zokukhishwa kwe-ultrasonic, amasampula ama-20g wezinto ezomile zamahlamvu kagwayi akhishwa ngamanzi afakwe emanzini ngamazinga okushisa aqala ku-5 ° C kuya ku-30 ° C. Ukuhlolwa kwesizinda sokudonswa kwemvelo kwenziwa ngezikhathi zokujikeleza kwesikhathi sokukhishwa okuhluka kusuka kumizuzu emi-5 kuya kwemizuzu engama-30 ngomjikelezo ngamunye. Cishe amaconsi ama-ethanol ayi-10 kuye kwangama-15 afakiwe emthanjeni wamanzi we-256 ml obaliwe wokudonsa wonke umthamo we-chlorogenic acid kumasampula angama-20 g amaqabunga kagwayi omisiwe. Umthamo omkhulu wamanzi we-256 ml wabe usuhlukaniswa waba ngamavolumu we-solvent wokuqothula ukuze usetshenziswe kumjikelezo ngamunye wokukhipha i-ultrasonic.
Ngokuba irradiation ultrasonic, iprosesa ultrasonic UP400S (Watts 400, 24 kHz) ne-sonotrode H7 eyenziwe nge-titanium, eyayine-tip ububanzi obuyi-7mm, yasetshenziswa. I-sonotrode H7 yayifakwe ekuphakanyeni kwesigamu se-extraction.
I-Protocol: Njengoba izimo ezilungile ze-ultrasonic isizinda se-chlorogenic acid kusuka emaqabunga kagwayi atholakala: amahlamvu angama-20g omisiwe kagwayi emanzini acwecwe (amanzi aphezulu kakhulu kuya kwesilinganiso esiqinile: 12,8mL / g) ku-20 degC processing temperature, i-ultrasonic device UP400S (400W, 24kHz) emijikelezweni engu-3 yomsindo we-15min ngayinye (ukuphela kwesikhathi sonication: 45 min).
Incomo yedivayisi:
UP400S nge-sonotrode H7
Isikhombo / Iphepha Lokucwaninga:
UMazvimba, uMartin Tongai; Yu, Ying; I-Zhang, i-Ying (2011): Ukulungiswa Nokuklama Kwe-Orthogonal Ye-Ultrasonic Esizakalayo Yokwakheka Kwamanzi Okucubungula Ukukhipha I-Chlorogenic Acid Kusuka Emanzini Egwayi Omanzi. I-Chinese Journal of Medicine Natural 2011.

Mangiferin acylation

Isicelo se-Ultrasonic:
I-Mangiferin (1,3,6,7-Tetrahydroxy-2- [3,4,5-trihydroxy-6- (hydroxymethyl) oxan-2-yl] xanthen-9-eyodwa; ifomula: C19H18O11) i-polyphenol ye-C-glycosylxanthone isakhiwo esingatholakala ezinhlotsheni eziningi zezitshalo. I-Mangiferin ibonisa imisebenzi ehlukahlukene yokwelapha. I-regioselective acylation ye-mangiferin ingasetshenziswa ngokucophelela kakhulu nge-lipase ngaphansi kwe-ultrasonication. Uma kuqhathaniswa nezindlela ezijwayelekile, i-catalysis eyayisizwa nge-ultrasonically ihamba phambili ngezinzuzo zesikhathi esifushane sokuphendula isikhathi kanye nezitshalo eziphakeme. Izimo ezinhle ze-ultrasonic mangiferin acylation zitholakala njengezilandelayo:
lipase: PCL, acyl donor: i-vinyl acetate; yokusabela solvent: DMSO, ukusabela kokushisa: 45 degC, amandla ultrasonic: 200W; i-substrate ratio: i-acyl donor / mangiferin 6/1, ukulayisha i-enzyme: 6 mg / ml
I-regioselective acylation isivuno yayingama-84%.
Incomo yedivayisi:
UP200St noma UP200Ht
Isikhombo / Iphepha Lokucwaninga:
cp .: Wang, Z .; Wang, R .; I-Tian, ​​J .; I-Zhao, B; I-Wei, i-XF; I-Su, YL; Li, CY; I-Cao, SG; Wang, L. (2010): Umphumela we-ultrasound ku-lipase-catalyzed regioselective acylation of mangiferin ezingxenyeni ezingenayo i-aqueous solvents. J. Asian Nat Prod. Res. 12/1, 2010. 56-63.

I-Capsaicinoids

Isicelo se-Ultrasonic:
Ukwenziwa kwe-capsaicinoids (i-capsaicin, i-nordihydrocapsaicin) kusuka e-chilipelepele: Ama-Capsaicinoids avela I-Capsicum frutescens i-pepper yatholakala nge-ultrasonic isizinda ngaphansi kwezimo ezilandelayo: i-solvent: 95% (v / v) ethanol, i-solvent / mass ratio ye-10 ml / g, i-40 min. sonication isikhathi extraction, 45 ° C lokushisa lokushisa. Ukuvunwa okweqile: 85% we-capsaicinoids
Incomo yedivayisi:
UP400S

Silymarin kusuka eMilk Thistle Seeds

Isicelo se-Ultrasonic:
Isizinda esisizwa nge-ultrasound senziwa ngokusebenzisa i-ultrasonicator UP400S. Kulesi sifundo ucwaningo lwe-horn-type ultrasonic lwalusetshenziswa ngobubanzi buka-1.5 cm. I-10 g ye-nonfat ubisi lwe-thistle powder yalinganiswa ngokunembile futhi yahlakazwa ku-100 ml we-methanol. I-beaker yayigcinwe ekugezeni kwamanzi futhi igcinwe ekushiseni kwama-25 ° C. I-milk thistle powder encibilikisiwe yabe isetshenziswe ngezikhathi ezahlukene (30, 60, 90, 120 no-150 min.) Emva kwesikhathi ngasinye, isixazululo sasihlungwa ngephepha le-Whatman lokuhlunga futhi i-filtrate ngayinye (cishe i-80mL) yachithwa kusetshenziswa i-evaporator ejikelezayo kuze kufinyelele isisombululo se-30 mL futhi ukumiswa kwamakhemikhali e-phenolic kwalinganiswa nge-UV-Visible Spectrophotometer ngobude obungama-517 nm ngokusho kwe-DPPH. Njengoba izikhathi zokwanda ezilindelekile zikhuphuka ekukhuleni kokuqukethwe kwe-silymarin.
Abalobi belo cwaningo bathola ukuthi isizinda se-ultrasonic ukuze uthole iziqhumane ezidakayo zenye yezindlela ezinhle kakhulu zokufaka esikhundleni samasu avamile. Isizinda se-ultrasonic sasiqinisekisiwe ukuthola ikhwalithi engcono yokukhipha. Izinzuzo ezengeziwe ukugcina isikhathi ngokusebenza kahle njll.
Incomo yedivayisi:
UP400S
Isikhombo / Iphepha Lokucwaninga:
I-Çağdaş, E .; Kumcuoğlu, S .; I-Güventürk, i-S.; I-Tavman, S. (2011): Ukufakelwa kwe-Ultrasound-Okusiziwe kwe-Silymarin Components kusuka ku-Milk Thistle Seeds (Silybum Marianum L.). IGIDA 36/6, 2011. 311-318.

Stevioside

Isicelo se-Ultrasonic:
Ama-sampuli ama-10g omile anama-stevia amaqabunga asemhlabathini athathwa ngamanzi angama-100mL ngaphansi kokugqugquzela okuqhubekayo (nge-stirrer magnetic). I-pH value yayilawulwa nge-0.01 M pH 7 ye-phosphate ye-sodium. Isampula yayifakwe ku-glass beaker eyi-150 ml kanye ne-ultrasonicator ye-probe-type (UIP500hd, 20kHz, 500W). Iphuzu le-sonotrode labhapathizwa cishe ngo-1.5 cm kuya emagqabini ase-stevia. Idivayisi ye-ultrasonic isethelwe amandla okukhishwa kwe-350W. Ukwelashwa okunomusa kwe-350 W kwe-5-10 min. ekushiseni okuqhubekayo kwama-30 ° C wanikezela i-rebaudioside A isivuno se-30-34g ngesampula se-100g. Ngemuva kwe-sonication, ikhambi lokukhipha laliyi-centrifuged futhi lihlungiwe ngo-0.45 μm ulwelwesi lwe-microporous; i-filtrate ithathwe ukuhlaziywa kokuqukethwe kwe-rebaudioside. Ukukhiqizwa kokukhipha kwe-rebaudioside A okuphelele okuqukethwe kwahlaziywa yi-HPLC.
Nge-ultrasound-assisted isizinda esingenaso solvent-free, isivuno esikhulu se-rebaudioside A satholwa ngokuqhathaniswa nezindlela zendabuko yesikhumba ezifana nokushisa kwesakhiwo noma i-maceration.
Incomo yedivayisi:
UIP500hd

Terpenes

Isicelo se-Ultrasonic:
Ukukhishwa kwamakhemikhali ahlukahlukene, njenge-terpenes, kusukela kuma-inflorescences we-fiber uhlobo lwe-Cannabis sativa L. cultivar lungathuthukiswa nge-ultrasonically. Ucwaningo luye lwabonisa ukuthi emva kwe-sonication emfishane ama-terpenes ahlosiwe kakade akhululwe kulesi sitshalo.
Imiphumela ibonisa ukuthi ukwelashwa kwe-ultrasonic okungavamile kunamaminithi angu-5 kuvumela ukuthola ukuhlushwa okuthuthukisiwe kwe-terpenes uma kuqhathaniswa ne-maceration. Esikhundleni salokho, ukwelashwa kwe-ultrasonic isikhathi eside kunamaminithi angu-5 kwandise ukuhlushwa kwe-δ-9-tetraidrocannabinol (THC).
I-Sonication protocol:
Isizinda esisizwa nge-ultrasound se-terpenes senziwa nge-a I-200W ye-probe-type ultrasonicator. Idivayisi ye-ultrasonic isethwe kuma-amplitude angu-25%. Kwakuhlanganiswa ama-aliquots amathathu ka-50g we-inflorescences omisiwe ngamunye nge-250mL ka-70% i-ethanol v / v, esetshenziselwa ukususa i-solvent. I-beaker ngayinye kanye nokuqukethwe kwayo kwabhapathizelwa ekugezeni kweqhwa. I-Sonication yenzelwe u-5, 10 no-15 min. Ngesikhathi Isizinda se-Ultrasonic inqubo, ukuqhuma kokushisa okusheshayo okusheshayo kuqinisekiswe ukuthi kugcinwe izinga lokushisa elingaphansi kuka-30 ° C. Ngemuva kokukhipha, amaxube ahlungiwe ngaphansi kwe-vacuum nge-Whatman No. 3 ephepheni, futhi i-solvent isusiwe ukuphuma kwe-rotary vacuum. Isivivinyo ngasinye sesisindo senziwe ngo-triplicate usebenzisa amasampuli amathathu ahlukene.
Ukutholakala kwe-ultrasonication kwatholakala ukuthi kuyindlela ekhangayo ekwenzeni i-maceration yokukhipha ama-volatile compounds kusuka ku-cannabis inflorescence, kodwa ikhombisa futhi ukuthi ukwelashwa kwe-ultrasonic akufanele kube mude kunamaminithi angu-5 ukuthola ukuvuselelwa okuthuthukisiwe kwe-terpenes. Esikhundleni salokho, ukwelashwa kwe-ultrasonic isikhathi eside kunemizuzu engu-5 kwande ukuhlushwa kwe-δ-9-tetraidrocannabinol (THC). Ukukhishwa kwe-Cannabis sativa inflorescences etholakala yi-ultrasonic isizinda eyenziwa ama-5 min kungasetshenziswa njengesithako se-perfume noma ukunambitheka kweziphuzo. Ingasetshenziswa njengezinye izindlela ze-hemp yezimboni, lapho kusetshenziselwa khona umquba embonini ye-fiber kanye ne-inflorescences okukhipha imithi yezimonyo nezokudla.
Incomo yedivayisi:
UP200St noma UP200Ht nge sonotrode / probe S26d14
Isikhombo / Iphepha Lokucwaninga:
Da Porto, C .; Decorti, D .; Natolino, A. (2014): “Isizinda esisizwa nge-ultrasound yamakhemikhali ahlukahlukene avela kwizimboni ze-Cannabis sativa L. inflorescences”. IJARNP 2014, 7 / 1. 8-14.

Ukususwa kwe-Vanillin kusuka kubhontshisi we-vanilla ophulukisiwe

Isicelo se-Ultrasonic:
Ukukhipha i-Vanillin kwakulungiswe ngaphansi kwe-sonication. Ngakho-ke, idivaysi engu-100 watts ultrasonic (20kHz) yaqhutshwa kwimodi ye-pulsed (imijikelezo: isekhondi elingu-5 kulandelwa ngu-5 sec.
Imiphumela emihle yatholakala nge-ultrasonic isizinda ngo-40% ethanol nge-1h e-30 ° C. Ukuqhathanisa imiphumela ngezindlela ezivamile zokutshala, ukugeza kwamanzi kanye nokukhishwa kwe-ultrasound yokugeza kubuye kwenziwe. Imiphumela yabonisa ukuthi ukuhlelwa kwe-vanillin ukukhipha nge-ultrasonic uphondo nge-40% ethanol for 1h at 30 ° C kwakuqhathaniswa amanzi okugeza isizinda nge 40% ethanol at 56 ° C for 15h.
Incomo yedivayisi:
UP100H
Isikhombo / Iphepha Lokucwaninga:
I-Rasoamandrary, N .; Fernandes, AM; Bashari, M .; Masamba, K .; I-Xueming, i-X. (2013): Ukukhipha okuthuthukisiwe kwe-Vanillin 4-Hydroxy-3-methoxybenzaldehyde kusuka kubhontshisi abavuthiwe abasebenzisa i-Ultrasound-Assisted Extraction: Ukuqhathaniswa kwe-Ultrasound-Assisted and Hot Water Bath Extraction. Akademik Gıda 11/1, 2013. 6-12.

Ziziphus Jujube - ukwenziwa kwamakhemikhali e-phenolic of jujube

Isicelo se-Ultrasonic:
Ngokuba isizinda se-ultrasonically esisizwa sama-phenolic ama-jujube (i-Ziziphus Jujube), i-high intensity probe-type ultrasonicator UP200H kwasetshenziswa amandla angu-200W no-24kHz. Idivaysi ye-ultrasonic yayine-sonotrode S2 encane (tip ububanzi obuyi-2 mm), eyabhapathizwa emanzini okugezela lapho isilazi esikhuphuka khona isampuli (ubukhulu bangaphakathi: 280: 195: 135 mm). Ukuphakama kwama-vibrations e-ultrasonic kwakungamaphesenti angu-100 wamandla okuqoka (amandla angu-260 μm) kanye namandla angu-0.171402 W kanye namandla angu-21.8346 W / cm2 .Ukuqina kwe-ultrasonic kunqunywe ngekhilomimimethrically ngokulinganisa ukwanda kwezinga lokushisa kwesikhathi sokumisa ngaphansi kwezimo ze-adiabatic . Inqubo yesikhumba se-ultrasonic yasetshenziselwa ukukhishwa kwama-phenolics angama-sampuli ngokusho komklamo wokuhlola (ukulungiselela).
Incomo yedivayisi:
UP200H nge sonotrode S2
Isikhombo / Iphepha Lokucwaninga:
I-Fooladi, H .; I-Mortazavi, SA; U-Rajaei, A .; U-Elhami Rad, i-AH; I-Salar Bashi, D .; Savabi Sani Kargar, S. (2013): Ukwandisa ukukhishwa kwama-phenolic compounds of jujube (Ziziphus Jujube) usebenzisa indlela yokusiza i-ultrasound. IECFP 2013.

Chofoza lapha ukuze uthole izivumelwano eziningi zokuxhumana nge-ultrasonic ukwelashwa kwezinto eziphilayo!

Isizinda se-ultrasonic singenziwa ekusebenzeni kwe-batch nokuqhubeka kokugeleza-kwemodi. (Chofoza ukuze ukhulise!)

Ukusethwa kwe-Sonication nge I-UIP1000hd ngenxa yesikhumba esivela emaqabeni e-boldo ku-batch. [Petigny et al. 2013]

Xhumana Nathi / Cela Ukwaziswa Olwengeziwe

Khuluma nathi mayelana nezidingo zakho zokucubungula. Sizosikisela ukusetha nokulungiswa kwemingcele efanele yephrojekthi yakho.





Sicela uqaphele wethu Inqubomgomo yobumfihlo.




Amaqiniso Okufanele Ukwazi

I-ultrasonic izicubu ze-homogenizers ngokuvamile ibizwa ngokuthi i-sonicator yesondlo, i-lyser ye-sonic, i-ultrasound ephazamisayo, i-grinder ye-ultrasonic, i-sono-ruptor, i-sonifier, i-sonic dismembrator, i-cell disrupter, i-ultrasonic disperser noma i-dissolver. Amagama ahlukene avela ezinhlobonhlobo zokusebenza ezingagcwaliseka nge-sonication.