I-Hielscher Ultrasound Technology

I-Ultrasonic-Enzymatic Oil Hydrolysis

  • Amafutha acebile kaDiacylglycerol (DAG) ayisithako esibalulekile sokudla, i-pharma nemikhiqizo yezimonyo.
  • I-Diacylglycerol ingakhiqizwa yi-hydrolysis yamafutha esundu ngokusebenzisa i-lipase yezohwebo njenge-catalyst ngaphansi kwe-ultrasonication.
  • Nge-hydrosonic-enzymatic hydrolysis, ama-DAG angakhiqizwa ngeminyango ephezulu ngezindleko eziphansi futhi ngesikhathi esifushane.

Ukukhiqizwa kwe-Ultrasonic-Enzymatic Diacylglycerol

I-Diacylglycerol (DAG) amafutha acebile asetshenziselwa ukudla, ama-pharma kanye nezicelo zezimonyo. Banesithakazelo esikhulu ngenxa yenani labo eliphakeme lokudla okunomsoco njengoba begaywa futhi behlelwa ngendlela enomzimba, okunciphisa kakhulu isisindo somzimba.
Nge-hydrolysis eyasiza kakhulu i-bio-catalyzed, amafutha yemifino ejwayelekile angashintshwa abe amafutha a-DAG acebile. I-ultrasonic-enzymatic hydrolysis iholela ekuvuneni okuphezulu kweoli e-diacylglycerol ecebile ngezikhathi ezimfushane zokuphendula futhi ngaphansi kwezimo ezinzima.
Inhlanganisela ye-ultrasound ne-catalysis enzymatic ingasetshenziselwa ukuthuthukisa amafutha ajwayelekile, isib. Amafutha omnqumo, amafutha anezinto eziphezulu ze-diacylglycerol. Okuqukethwe okuphezulu kwe-diacylglycerol kunika amafutha inani eliphezulu lokudla okunomsoco.

Izinzuzo ze-ultrasound:

  • i-emulsification enhle
  • ukudluliswa kwamandla amaningi
  • ukuguqulwa okuphezulu
  • izimo ezinomusa
  • isikhathi esifushane senqubo
  • ukushisa okulawulwayo
  • ukukhiqizwa ngaphakathi
Isakhiwo se-diacylglycerol (DAG) lapho i-R1, R2, no-R3 kuyi-alkyl noma i-alkenyl, i-hydrocarbon chain ye-fatty acid (ifomula jikelele R-COOH).

Isakhiwo se-diacylglycerol (DAG)

I-ultrasonication iyaziwa kakhulu ukuthuthukisa ukudluliswa kwamandla

Ultrasonic ingilazi yeglasi, isb for emulsification

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Ucwaningo & Imiphumela

Awadallak et al. (2013) baye baphenya nge-ultrasonically basiza i-hydrolysis yamafutha esundu ngama-Lipozyme RM IM njenge-biocatalyst. Esikhathini esithatha izinyathelo ezimbili, i-ultrasound isetshenziselwa ukukhuthaza i-emulsification yamafutha namanzi. Esinyathelweni sesibili, ama-enzyme ayengezwa ukuguqulwa kokucubungula.
Isithombe ngakwesokudla sibonisa ukusetha kwe-ultrasonic njengoba kusetshenziselwa ucwaningo lwe-Awadallak: idivayisi ye-ultrasonic probe UP200S (200W, 24kHz) nge-glass flow cell ukuze i-sonication eqhubekayo ngaphansi kwezimo ezilawulwayo.

I-Protocol

Iqembu lokucwaninga lithole ukuthi ukulandela imiphumela yezinyathelo ezimbili kulemiphumela emihle kakhulu: Ukusabela kwenzelwa ku-ultrasonic glass flow flow cell nge-60ml ivolumu (bheka isithombe ngakwesokudla) ku-55 ° C ngehora lesishiyagalombili. Amafutha ompalm (15 g) namanzi (1.5 g) wanezelwa ku-reactor. Inqubo ye-ultrasound ye-ultrasonicator UP200S lafakwa ku-10mm ekujuleni kwamanzi / ohlelweni lwamafutha, amandla ahlelwe ku-80W futhi avuliwe amaminithi amathathu ukuze kusetshenziswe uhlelo ngaphambi kokuba asuswe, bese i-enzyme (1.36 wt.% amanzi wamanzi +) wanezela ngenkathi isixazululo sasihlanganiswa nokuvuthwa kwamagnetic (300 rpm).
Ngakho-ke ama-bio-catalysis asetshenzisiwe nge-ultrasonically akhiqiza amafutha e-DAG nge-34.17 wt.% Ukuhlushwa ngemva kwesikhathi esingu-12h sokuphendula. Isinyathelo sonication ngokwayo sasincane kakhulu isikhathi esingamaminithi angu-1.2 kuphela.

Imiphumela

Ezilingo ezinikezwe, amafutha e-DAG anama-34.17 wt.% Okuhlushwa atholakala emva koku-12 h ukuphendula. Isinyathelo sonication sithatha 1.2 min kuphela.
I-ultrasonic-enzymatic catalysis iyaqiniseka ngezinzuzo zayo ezinkulu zokukhiqiza okukhulu, njengoba izindleko zayo zamandla ziphansi kakhulu futhi isikhathi sayo esifushane sokuthi i-emulsification ivumela ukusetshenziswa kokunciphisa imishini ye-ultrasonic ekwehliseni izidakamizwa ezinkulu ze-hydrolysis. [Awadallak et al. 2013]

I-UP200S nge-glass reactor ye-hydroxysis eyakhiwe nge-ultrasonically ye-hydrolysis yamafutha

Idivayisi ye-ultrasonic probe UP200S nge-glass reactor

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Izincwadi / Izinkomba

  • Adewale, uPeter; UDumont, Marie-Josée; Ngadi, Michael (2015): synthesis enzyme-catalyzed synthesis kanye kinetics of ultrasonic-basizwa biodiesel ukukhiqizwa kusuka imfucuza tallow. Ultrasonics Sonochemistry 27; 2015. 1-9.
  • I-Awadallak, i-Jamal A .; U-Voll, uFernando; I-Ribas, uMarielen C .; da silva, camila da; Filho, Lucio Cardozo; da Silva, Edson A. (2013): I-enzymatic catalyzed oil hydrolysis ngaphansi kwe-ultrasound irradiation: i-Diacylglycerol synthesis. Ultrasonics Sonochemistry 20; 2013. 1002-1007.
  • I-Dhara R .; Dhar P .; I-Ghosh M. (2013): Imiphumela yokudla ka-diacylglycerol ocebile oyeladi lwesinaphi kwi-lipid profile yama-rats normocholesterolemic nama-hypercholesterolemic. Journal of Food Science Technology 50 (4); 2013. 678-86.
  • I-Dhara R .; Dhar P .; I-Ghosh M. (2012): Imiphumela yokudla ye-oli ehlanzekile ne-diacylglycerol ene-rice bran oil ekwenzeni ukukhula kanye nephid profile yezinja. Journal of Oleo Science 61 (7); 2012. 369-75.
  • UGoncalves, uKaren M .; Sutili, Felipe K .; I-Leite, i-Selma GF; de Souza, u-Rodrigo OMA; URamos Leal, i-Ivana Correa (2012): i-hydrolysis yamafutha e-palm ekhishwa yi-lipases ngaphansi kwe-ultrasound irradiation - Ukusetshenziswa komklamo wokuhlola njengethuluzi lokuhlola okuguquguqukayo. Ultrasonics Sonochemistry 19; 2012: 232-236.
  • U-Souza, u-Rodrigo OMA; I-Babicz, i-Ivelize; I-Leite, i-Selma GF; I-Antunes, i-Octavio AC: I-Lipase-Catalyzed Diacylglycerol Ukukhiqizwa Ngaphansi Kwe-Sradichemical Irradiation.
  • I-Nagao T .; I-Watanabe H .; I-Goto N .; Onizawa K .; I-Taguchi H .; I-Matsuo N .; Yasukawa T .; I-Tsushima R.; Shimasaki H .; I-Itakura H. (2000): I-diacylglycerol yezinkukhu igxilisa ukuqoqwa kwamafutha omzimba kuqhathaniswa ne-triacylglycerol emadodeni ekuvivinyweni okulawulwa kabili. I-Journal of Nutrition 130, 2000. 792-797.


Amaqiniso Okufanele Ukwazi

Mayelana ne-Diacylglycerols
I-Diacylglycerols (DAG) isetshenziswe ngokujwayelekile emazingeni ahlanzekile ahlukahlukene njengezithasiselo zokuthuthukisa i-plasticity yamafutha noma izisekelo zokudla, imithi nezimboni zezimonyo. I-DAG nayo isetshenziselwa amafutha e-estranger ukuhlukanisa izinto ezivela ezibunjiweni kanye nokuguqula amafutha, ama-precursor for synthesis organic products ezifana ne-phospholipids, i-glycolipids, i-lipoproteins, izidakamizwa ezinjenge-DAG-i-chlorambucil ephathekayo yokwelapha i-lymphoma, (S ) - (3.4-dihydroxyphenyl) alanine (i-LDOPA) yokwelashwa kwesifo sikaParkinson nabanye abaningi. Muva nje, amafutha a-DAG asele asetshenzisiwe njengamafutha okupheka asebenzayo, anokuqukethwe okungenani okungu-80% we-1,3-DAG. [Nagao et al., 2000]
I-Diacylglycerol (DAGs) ingakhiwa yi-hydrolysis, ingxenye ye-esterification noma i-glycerolysis ngokusebenzisa i-chemical or enzymatic catalysis. I-catalysis ye-enzymatic iyindlela ekhethiwe kusukela ingenziwa ngaphansi kwezimo ezinesizotha (izinga lokushisa eliphansi kanye nocindezelo).