I-Hielscher Ultrasound Technology

I-Ultrasonic Collagen Extraction

  • I-Collagen inamaprotheni amaningi futhi isetshenziselwa kabanzi kwizicelo ezimbalwa zezimboni, isib. Ukudla, i-pharma, izithasiselo njll.
  • I-Sonication ingahlanganiswa kalula ne-enzymatic noma i-acid yesikhumba se-collagen.
  • Ukuqaliswa kwe-ultrasonics kuhlelo lokuhlanganiswa kwe-collagen kubangelwa ukuvunwa okuphezulu kanye nokukhishwa ngokushesha.

Imiphumela ye-ultrasonic ku-Extract Collagen

I-high-intensity ultrasound isetshenziselwa ukuthuthukisa ukudluliselwa kwamanzi ezinkambeni ezimanzi, isib. Ukukhipha, i-sonochemistry njll. Isizinda (esaziwa ngokuthi i-collagen isolation) se-collagen singathuthukiswa kakhulu ngokuphathwa kwe-ultrasonic. Izinsiza ze-Sonication ngesikhathi se-collagen substrate, ivula ama-collagen fibrils, ngakho-ke i-hydrolysis ene-enzymatic noma i-asiriya yokwelashwa yenziwa lula.

Isisetshenziswa se-Ultrasonically esixhaswe nge-Enzymatic

I-Sonication yaziwa ngokukwazi kwayo ukwandisa umsebenzi we-enzyme. Lo mphumela usekelwe ekuhlakazeni kwe-ultrasonic nokuguqulwa kwe-aggregate ye-pepsin. Ama-enzyme ahlakazekile ngokulingana ahlinzekela indawo ekhulayo yokudlulisa okuningi, okuhambisana nomsebenzi ophezulu we-enzyme. Ngaphezu kwalokho, amagagasi anamandla ama-ultrasound avula ama-collagen fibrils ukuze i-collagen ikhishwe.

I-ultrasonic Pepsin Extraction: I-Pepsin ihlangene ne-ultrasonication kwandisa isivuno se-collagen kuze kube ngu-approx. 124% futhi ikhulula kakhulu isikhathi sokukhipha uma kuqhathaniswa ne-pepsin hydrolysis evamile. Ukuhlaziywa kwe-circular dichroism, i-atomic force microscopy kanye ne-FTIR yabonisa ukuthi isakhiwo esine-triple helix sogesi esakhiwe asizange sithinteke yi-sonication futhi sahlala singanyaka. (Li-al., 2009) Lokhu kwenza ukuba isizinda se-pepsin esixhaswe nge-ultrasonically sisebenze kakhulu ekuhlinzekeni kwemikhiqizo yokudla okwandisa izinga lokuthola amaprotheni ngesikhathi esifushane sokucubungula.

Esifundweni sokuqhathanisa nesimiso se-ultrasonic esingafani ne-ultrasonic se-collagen kusuka ku-tendon ye-bovine, ukwelashwa kwe-ultrasound (20kHz, i-pulse mode 20/20 sec.) Eqinisekisiwe ngokuvunwa okuphezulu nokusebenza kahle. Isizinda esivamile senziwa nge-pepsin e-acetic acid amahora angu-48. Isizinda se-ultrasonic senziwa isikhumba ngaphansi kwezimo ezifanayo, kodwa izikhathi zokungcebeleka ku-sonication (3 kuya ku-24 h) kanye ne-pepsin (amahora angu-24 kuya ku-45) ahlukahlukene, okuholela ekutholeni kwamahora angu-48 yokwelashwa. I-ultrasonic-i-pepsin isizinda yabonisa ukusebenza kahle okuphakeme komshini we-collagen, kufinyelela isivuno sika-6.2%, lapho isivuno esivamile sesivuno saba ngu-2.4%. Imiphumela emihle yatholakala ngesikhathi sokuqothula i-ultrasonic esebenzisa u-18 h. I-collagen ekhishiwe ibonise isakhiwo esiqhubekayo se-helix esingahleliwe, isilungele kahle nokuqina okunamandla okukhulu. lokhu kusho ukuthi i-ultrasonic-i-pepsin isizinda sithuthukise ukusebenza kahle kwe-collagen yemvelo ngaphandle kokulimaza ikhwalithi ye-collagen ephumela. (Ran and Wang 2014)

Isethaphu ye-ultrasonic nge-tank eshukumisayo

Isicelo solwazi




Qaphela kwethu Inqubomgomo yobumfihlo.


I-ultrasonic Papain Extraction: I-Collagen evela esikalini sezinhlanzi ingasuswa ngokuphumelelayo yi-papain hydrolysis ehlangene ne-ultrasonic pre-treatment. Ngokuba isisetshenziswa se-ultrasonic-papain se-collagen kusuka esikalini sezinhlanzi kwakuyizinqubo ezilandelayo ezifunyenwe njengezinga eliphezulu: ubude be-ultrasonic ngaphambi kwesikhathi se-4min, isilinganiso se-papain ukudoba isikali esingu-4%, izinga lokushisa elingama-60 ° C kanye nesikhathi esiphezulu sokukhipha u-5h. Ngaphansi kwezimo ezinhle kakhulu izinga le-collagen lafinyelela ku-90.7%. (Jiang et al. 2011)

Ultrasonically Assisted Extraction Extraction

Esifundweni sikaKim et al. (2012), ukukhishwa kwe-collagen e-acid-soluble esikhunjeni se-Japanese sea bass (i-Lateolabrax japonicus) kubonise isivuno esikhulu futhi sanciphisa isikhathi sokukhipha ngemuva kokuphathwa kwe-ultrasonic ngesivinini se-20 kHz ku-0.5 M i-acetic acid. Ukukhipha nge-ultrasound akuzange kushintshe izingxenye ezinkulu ze-collagen, ikakhulukazi izinketho ze-α1, α2 ne-β.

I-Hielscher inikeza amadivaysi anamandla ama-ultrasonic kusukela ebhodini kuya esikalini sezimboni (Chofoza ukuze ukhulise!)

Izinqubo ze-Ultrasonic: Kusuka I-Lab kuya Izimboni Isikali

Ukukhishwa kwe-ultrasonic yamaProtheyini avela kuma-Egg Shells

Ama-hydrolysates e-enzymatic ngaphambili aphethwe yi-ultrasonically ayenempahla engcono yokusebenza. Ukuze uthole i-ultrasonic isizinda seprotheyini hydrolysates evela enkukhu amaqanda igobolondo, i-solubility, i-emulsifying, i-foaming ne-water holding properties izithuthukiswa.
Isikhwama se-Eggshell yisisetshenziswa semvelo esiningi futhi sinamaprotheni angaba ngu-64 kuhlanganise nohlobo lwe-I, V ne-X collagen, i-lysozyme, i-osteopontin, ne-sialoprotein. Lokhu kwenza ama-eggshell abe indaba eluhlaza okwakusetshenziselwa ukukhipha amaprotheni. Nge-ultrasonic isizinda, ukukhululwa kwamaprotheni kanye nokusebenza kungathuthukiswa kakhulu kubangelwa inqubo esheshayo, ephumelelayo neyokonga.

I-Ultrasonically Assisted Extraction Extraction

ukuze akhiphe futhi ahlaziye lawa maprotheni
Ukuze ukhiqizwe amaprotheni kusuka embhedeni we-eggshell, ukwelashwa kwe-ultrasonic-alkali kwaholela ekubeni amaprotheni anelulisiwe akhiphe cishe amaphesenti angu-100 weprojekthi yamaphrotheni ye-eggshell. I-cavitation ye-Ultrasonic ifihliwe amaprotheni amakhulu kakhulu aphuma embundwini we-eggshell futhi yenza lula ukuhlanganiswa kwamakhemikhali ayo. Isakhiwo seprotheyini nezakhiwo azizange zonakaliswe yi-sonication futhi zahlala zinganyakazi. Izakhiwo ze-antioxidant zamaprotheni zazifana nokuphathwa kwe-alkali-ultrasonic futhi kusiza okuvamile.

Ultrasonic Gelatin Extraction

Izikhumba ze-pollock ezomileyo nezomile zomoya zathathwa ngama-saline abandayo, ama-alkaline kanye ne-asidi ukuze ahlukanise izicubu ze-collagen futhi akhiphe i-gelatin nge-collagen denaturation ku-45 ° C amahora amane ngokuphathwa kwe-ultrasound njengesiza sokusiza. I-Gelatin isivuno, i-pH, icacile, amandla we-gel nezakhiwo ze-viscoelastic kanye nokusabalalisa kwesisindo se-molecular esinqunywe yi-PAGE-SDS indlela yahlolwa. I-gelatin ekhishwe ngamanzi okugeza ngamanzi angu-45 ° C amahora amane isetshenziselwa ukulawula. Ukwelashwa kwe-ultrasound yamandla kwandise isivuno se-extraction ngo-11.1% uma kuqhathaniswa nokulawula ngenkathi amandla e-gel awela ngo-7%. Ukushisa kwe-gelation kwakungaphansi kwe-ultrasound-i-gelatin ekhishiwe (4.2 ° C). Lokhu kuziphatha kuhlobene nokwehlukana kokusabalalisa kwesisindo samangqamuzana amakhoyili we-polypeptide kuma-gelatini. Isisetshenziswa se-ultrasound amandla singasetshenziselwa ukwandisa isikhala se-gelatin kusuka ezikhumba zezinhlanzi ezibandisiwe nezomoya. (Olson et al. 2005)

izinhlelo ze-industrial ultrasonic

I-Hielscher Ultrasonics inikeza izinhlelo ezinamandla ze-ultrasonic kusukela ebhodini kuya ebhentshini-phezulu kanye nezilinganiso zezimboni. Ukuze kuqinisekiswe ukukhishwa kwezinga eliphezulu, ukunikezwa kwezingane ezinokwethenjelwa ngaphansi kwezimo ezidingayo kungenziwa ngokuqhubekayo. Zonke izinqubo ze-ultrasonic zezimboni zingakhipha ama-amplitudes aphakeme kakhulu. Amplitudes angafika ku-200μm angaqhutshwa ngokuqhubekayo ku-24/7 ukusebenza. Nge-amplitudes ephakeme nakakhulu, i-sonotrode e-ultrasonic eyenziwe ngezifiso iyatholakala. Ukuqina kwemishini ye-ultrasonic ye-Hielscher kuvumela ukusebenza komsebenzi we-24/7 emsebenzini obuthakathaka nasezindaweni ezinzima.
Sicela uxhumane nathi namuhla ngezimfuneko zakho zenqubo! Sizojabula ukukuncoma uhlelo olufanelekayo lwe-ultrasonic lwenqubo yakho!

Cela ulwazi oluthe xaxa

Sicela usebenzise ifomu elingezansi, uma ufisa ukucela ulwazi oluthe xaxa mayelana ne-homogenization yama-ultrasonic. Sizojabula ukukunikeza uhlelo lwe-ultrasonic ukuhlangabezana nezidingo zakho.









Sicela uqaphele wethu Inqubomgomo yobumfihlo.


I-ultrasonic disperser iyatholakala kusuka kudivayisi encane egcinwe ngesandla kuze kube yilabhenki-phezulu kanye nezimo ezigcwele zezimboni ze-ultrasonic zokucubungula ivolumu enkulu (Chofoza ukuze ukhulise!)

Ama-homogenizers ama-ultrasonic high-power ama-ultrasonic atholakalayo kunoma iyiphi inqubo yesilinganiso – kusuka ebhodini kuya ekukhiqizeni.

Izincwadi / Izinkomba

  • U-Alvarez, uCarlos; ULélu, uPauline; ULynch, uSara A .; Tiwari, Brijesh K. (2018): Ukulungiswa kwamaprotheni okwenziwe yi-mackerel yonke inhlanzi ngokusebenzisa i-acid equqile / i-alkaline isoelectric solubilization precipitation (ISP) isizinda esisizwa yi-ultrasound. LWT – Isayensi Yezokudla Nezobuchwepheshe Vol. 88, Febhuwari 2018. 210-216.
  • I-Jain, i-Surangna; Kumar Anal, Anil (2016): Ukuhlelwa kokukhipha amaprotheni ama-hydrolysates avela ku-inkukhu amaqanda egobolondo (ESM) nge-ultrasonic isizinda (UAE) ne-hydrolysis enzymatic. LWT – Isayensi Yezokudla Nezobuchwepheshe Vol. 69, ngoJuni 2016. 295-302.
  • Kim, HK; Kim, YH; Kim, YJ; Ipaki, i-HJ; Lee, NH (2012): Imiphumela ye-ultrasonic ukwelashwa kwesiqhumane se-collagen kusuka ezikhumba zasolwandle uLateolabrax japonicus. I-Fisheries Science Science Volume 78, Issue 78; 2013. 485-490.
  • Li, Defu; I-Mu, i-Changdao; U-Cai, uSumei; I-Lin, Wei (2016): Irradiation ye-ultrasonic kusizinda se-enzymatic se-collagen. Ultrasonics Sonochemistry Umqulu 16, Issue 5; 2009. 605-609.
  • Olson, DA, Avena Bustillos, RD, Olsen, CW, Chiou, B., Yee, E., Bower, CK, Bechtel, PJ, Pan, Z, Mc Hugh, TH (2005): Ukuhlolwa kwamandla ultrasound njenge ukusiza usizo lwe-fish gelatin extraction. Ukuhlangana no-Abstract No. 71C-26. Umhlangano Wonyaka We-IFT. July 2005. New Orleans, LA.
  • Ukuhamba, i-XG; Wang, LY (2014): Ukusetshenziswa kokwelashwa kwe-ultrasonic ne-pepsin ngokukhipha isithwathwa se-collagen kusuka embonini yenyama ngemikhiqizo. Journal of the Science of Food and Agriculture 94 (3), 2014. 585-590.
  • Schmidt, MM; Dornelles, RCP; Mello, RO; Kubota, EH; I-Mazutti, MA; I-Kempka, i-AP; I-half, IM (2016): Inqubo yesikhamo se-Collagen. I-International Food Research Journal 23 (3), 2016. 913-922.
  • I-Siritientong, iTippawan; Bonani, Walter; I-Motta, i-Antonella; Migliaresi, Claudio; I-Aramwit, i-Pornanong (2016): Imiphumela ye-Bombyx mori silk nokususa isikhathi sokukhipha izici ze-molecular and biological ze-sericin. I-Bioscience, i-Biotechnology, ne-Biochemistry Vol. Ama-80, ama-Iss. 2, 2016. 241-249.
  • Zeng, JN; Jiang, BQ; I-Xiao, i-ZQ, i-Li, SH (2011): Ukukhishwa kwe-Collagen kusuka ku-Fish Scales ne-Papain ngaphansi kwe-Ultrasonic Pretreatment. Ucwaningo Lwezingqalasizinda Eziphambili, Umqulu 366, 2011. 421-424.


Amaqiniso Okufanele Ukwazi

I-Collagen

I-Collagen iyiprotheni eyakhiwe ngokuyinhloko endaweni ye-extracellular kulezi zicubu ezihlukahlukene ezixhumene nezidumbu zezilwane. Njengengxenye eyinhloko yezicubu ezixhunyiwe, yiyona amaprotheni amaningi kakhulu ezincelisayo, [1] okwakhiwa kusuka ku-25% kuya ku-35% we-body-body protein content. I-Collagen iqukethe ama-amino acid ahlanganiswe ndawonye ukuze enze ama-triple-helices ukwakha amafriji ahlanganisiwe. Inani eliphakeme kakhulu le-collagen lisezinhlakeni ezinamafutha afana nama-tendon, ligaments kanye nesikhumba. Kunezinhlobo ezintathu ze-collagen ezizohlukaniswa:
Thayipha i-collagen: inikeza amaprotheni ama-90% esikhumba, izinwele, izipikili, izitho, amathambo, izigulane
Uhlobo lwesi-collagen: inikeza amaprotheni angama-50-60% ku-cartilage, u-85-90% we-collagen ku-carticlage ye-articular
Uhlobo lwe-collagen: inikeza amaprotheni kuma-protein ane-fibrous at bone, cartilage, dentin, tendon, nezinye izicubu ezihlangene

I-Collagen Emzimbeni

Izinhlobo ezintathu ze-collagen zakhiwe eziprotheyini ezahlukene ezifeza izinhloso ezahlukene emzimbeni. Izinhlobo ze-collagen I no-III zombili ziyingxenye eyinhloko yesikhumba, imisipha, amathambo, izinwele kanye ne-nail. Kudingeka impilo, ukukhula nokuvuselelwa. Uhlobo lwe-collagen II luyatholakala kakhulu ku-cartilage namalungu.
I-Collagen yohlobo I no-III zombili ziqukethe ama-amino acid angu-19 abhekwa njengama-amino acid abalulekile. Zakhiwa yi-fibroblasts (amangqamuzana angama-tiscues ahlangene) nama-osteoblasts (amangqamuzana enza amathambo) .Amaprotheni abaluleke kakhulu ku-collagen uhlobo I no-III ahlanganisa i-glycine, i-proline, i-alanine ne-hydroxyproline. Uhlobo III luyi-scleroprotein ene-fibrous.
I-glycine yi-amino acid enenani eliphakeme kakhulu ku-collagen. I-Proline iyin-amino acid engeyona ebalulekile, engakhiwa kusuka ku-glycine futhi inika amajoyini nama-tendon. I-Hydroxyproline iyi-amino acid efaka isandla ekuqiniseni komgogeni. I-Alanine i-amino acid ebalulekile ekukhiqizeni i-biosynthesis yamaprotheni.
Njengahlobo I no-III, uhlobo lwama-collagen angu-2 lwakhiwa ama-fibrils. Le nethiwekhi ye-fibrillar ye-collagen ibalulekile ku-cartilage njengoba yenza ukuthi kube nokuhlanganiswa kwama-proteoglycans. Ngaphezu kwalokho, linikeza amandla okuqina emathunjini.

Imithombo kanye Nemisebenzi

I-Collagen iyiprotheni enesibindi ekhona kakhulu ezinxenyeni ezixhumene nezilwane ezincelisayo, isib. Isifuba, ingulube. Iningi le-collagen likhishwa
kusuka ezikhumba ze-porcine namathambo nasemithonjeni yezinkomo. Umthombo ohlukile wesitokisi se-collagen yizinhlanzi nezinyoni. I-Collagen isetshenziselwa kabanzi ekudleni, izithako zokudla, izidakamizwa / imithi, nezimonyo phakathi kweminye imikhiqizo. Isizinda se-collagen ibhizinisi elikhulayo kusukela le phrotheni ingashintsha ama-agent esebenza ngezinqubo ezihlukahlukene zezimboni.