I-Hielscher Ultrasound Technology

Ultrasonic Bioreactors for Fermentation

I-Ultrasonics iyindlela ephumelelayo yokuvuselela ama-microorganisms ngama-vibrations angomakhenikha kanye ne-cavitation. Ku-sonobioreactor / i-Fermenter ye-sonobiore, ukwelashwa kwe-ultrasonic kwamangqamuzana nezicubu kuguquguquka kakhulu ngoba izici zezemvelo zinganqunywa ngokuqondile. Ngama-bioreactors e-ultrasonic, umphumela wokufutheka ungathuthukiswa kakhulu.

Ukuvuthwa

Ukusebenza kahle kokuvutshelwa kuncike ezimweni zenkambiso: Ama-nutrients, ubukhulu bomphakathi, izinga lokushisa, okuqukethwe kwe-oxygen / gas kanye nokucindezelwa yizinto ezibalulekile ezithonya umsebenzi we-microbial. Ama-microorganisms kanye namaseli omamamalia akhula kuphela ngaphansi kwezimo ezithile. Izimo ezifanele zihlangene nokuvuselela kwe-ultrasonic kungenza ngcono isivuno sokuvutshelwa.

Ukukhuthazwa kwe-Ultrasonic yezinyunyana

I-UP400ST iyi-homogenizer ye-ultrasonic esetshenziselwa ukuqinisa ukuvutshelwa ngokwenza ngcono ukudluliselwa kweningi nokukhuthaza ukukhula kweseli okuncane.Ukuvuthwa kuyindlela yokwenza umzimba eguqula ushukela kuma-acids, gesi, noma utshwala. Kwenzeka emvubelo namabhaktheriya, futhi nakwamaseli e-muscle alambile, njengasendleleni yokuvutshelwa kwe-lactic acid. Ukuvuthwa kusetshenziselwa kabanzi ngokubanzi ukubhekisela ekukhuleni okunamandla kwamagciwane ezincane ezikhulayo, ngokuvamile ngenhloso yokukhiqiza umkhiqizo othile wamakhemikhali.
Inqubo yokuvuthwa yenziwa ngezinga lezimboni ngokusebenzisa ama-microorganisms njengama-bacteria ne-fungus for the fermentation. Imikhiqizo etholakalayo isetshenziselwa ukudla kanye nomkhakha jikelele. Amakhemikhali, njenge-acetic acid, i-citric acid, ne-ethanol akhiqizwa ngokuvutshelwa. Izinga lokuvutshelwa lithonywa ukuhlushwa kwamagciwane ezincane, amaseli, izingxenye zamaselula, kanye nama-enzyme kanye nokushisa, kanye ne-pH. Ukuvutshelwa kwe-aerobic, i-oxygen iyisici esibalulekile futhi. Cishe zonke izinhlobo ze-enzyme ezikhiqizwa kwezohwebo, njenge-lipase, i-invertase kanye ne-rennet, zenziwe ngokuvutshelwa nge-microbes eziguquguqukayo.
Ngokuvamile, ukuvutshelwa kungahlukaniswa zibe izinhlobo ezine:

  • Ukukhiqizwa kwe-biomass (okubonakalayo okusebenzayo kweselula)
  • Ukukhiqizwa kwemetabolite ye-extracellular (amakhemikhali amakhemikhali)
  • Ukukhiqizwa kwezingxenye ze-intracellular (ama-enzyme namanye amaprotheni)
  • Ukuguqulwa kwe-substrate (lapho i-substrate eguqulwe yikhona umkhiqizo)
Iprosesa le-Ultrasonic UIP2000hdT (2kW) elinamandla ashukumisayo we-batch

I-homogenizer ye-ultrasonic I-UIP2000hdT (2kW) nge-batch reactionor

Isicelo solwazi




Qaphela kwethu Inqubomgomo yobumfihlo.


Sonication Ngaphambi, Ngesikhathi nangemva Fermentation

Ultrasonic Pre-treatment – Ukuthuthukiswa kokutholakala kwe-Biomass

I-Sonication njengokwelashwa kwangaphambili isetshenziselwa ukukhuthaza ukudluliselwa kwesisindo futhi yenze i-substrate itholakale kakhulu kumagciwane. Ukuxuba kwe-Ultrasonic kukhuthaza ukudluliselwa kwenqwaba yama-substrates ku, nemikhiqizo kude, namaseli amancane. Ukuqiniswa kwe-Ultrasonic kokudlulisa okuningi kungasetjenziswa njengokwelashwa kwangaphambili nangesikhathi se-Fermentation.
Isibonelo, ukwenziwa kwe-ultrasonic kwe-iray hull kwakusetshenziselwa ukuthuthukisa i-enzymatic hydrolysis yokuqanjwa kwe-xylooligosaccharides (XOs) ngu-Aspergillus japonicus (var. Japonicus CY6-1). Ngokukhiqizwa kwe-sonication, ukukhiqizwa kwama-enzymes we-cellulolytic kanye ne-xylanolytic kusuka ku-irayisi hurey kukhuliswe kakhulu. Isithelo se-hemicellulose sandiswa saya ku-1.4-fold ngaphansi kwe-sonication futhi isikhathi sokukhiqizwa sancishiswa kakhulu ukusuka ku-24 h kuye ku-1.5 h ku-80ºC – kanye nokunye okusemandleni okusebenza kwenqubo. I-biomass ene-sonicated kulula ukuguqula i-fungi ukuze kuqinisekiswe umsebenzi we-enzyme futhi umsebenzi we-CMCase, b-glucosidase, ne-xylanase ukwandiswa uma uqhathaniswa nehotela elingenalo i-rice. Imikhiqizo yokugcina fermentative yayiyi-xylotetraose, i-xylohexaose, nesisindo esiphezulu se-molecular mass xylooligosaccharides. Isivuno se-xylohexaose esivela ku-helmese irayisi yayineminyaka engu-80 ephezulu.

Ultrasonic Fermentation – Ukukhuthazwa Kwezinto Ezincane

Hielscher's ultrasonicators are used to intensify fermentation processes, e.g. the biosynthesis of proteinsSonication ngokulawula kahle futhi ephindaphindiwe kusiza ekuthuthukiseni ukukhiqizwa kwezinqubo ezahlukene zokuvutshelwa ngaphandle kokulimaza amaseli. Ukuqina kwe-sonication kungashintshwa ngqo ezinhlobonhlobo zezilwane nezidingo zayo. Nge-sonication elawulwayo, ukukhula kwamaseli kanye nokusetshenziselwa umzimba kunomthelela omuhle futhi ukuguqulwa okubanjwe ngamaseli abukhoma kuthuthukiswa kakhulu, isib. Ukugqugquzela i-Bifidobacteria ebisi.
Eminye izinqubo zokuvutshelwa kwe-fungal, i-sonication isetshenziswa ngokuphumelelayo ukuguqula ukukhula kwe-morphology kanye ne-broth rheology ngaphandle kokuphazamisa izinga lokukhula kanye nokukhiqizwa kwefungi le-filamentous.

I-Ultrasonic Post-treatment – Ukukhipha

Ukukhiqizwa kwezingxenye ze-intracellular ezifana ne-microbial enzymes (isib. Catalase, amylase, protease, pectinase, glucose isomerase, cellulase, hemicellulase, lipase, lactase, streptokinase) namaphrotheni aphindaphindiwe isici, i-streptokinase), amaseli kufanele ahlaziye / aphazamiseke ngemva kwenqubo yokuvuthwa ukukhulula amaprotheni ayifunayo. Nge-sonication, ukukhishwa kwe-intracellular kanye ne-extracellular polysaccharide-protein complexes ezivela ku-viscous mycelial fermentation mhluzi. Ngaphandle kokukhiqizwa kwayo okuvelele kanye nokusebenza kahle, i-sonication isungulwe futhi inokwethenjelwa kwe-cell lysis kanye nokukhishwa kwendaba ye-intracellular.
Chofoza lapha ukuze ufunde mor mayelana lysis ultrasonic kanye isizinda!

Ultrasonic Bioreactors

Hielscher Ultrasonics yisikhathi eside ehlangene nezinqubo ultrasonically ezikhuthaziwe. Sinikeza izigameko eziningi ze-ultrasonic ezihlukahlukene nobukhulu be-geometri. Ngaphandle kwalokho, sinikeza izixazululo ezenziwe ngokwezifiso zokuhlanganiswa ku-bioreactor yakho ekhona. Njengoba amaprosesa ethu e-ultrasonic ahlukahlukene kakhulu futhi adinga isikhala esilinganiselwe kuphela, ukuphindaphinda kwezitshalo ezikhona ze-biotechnological kungenziwa ngaphandle kwezinkinga.
Ithebula elingezansi, libonisa izincomo zedivayisi jikelele ngokuya kwevolumu ye-batch noma izinga lokugeleza okufanele lisetshenziswe. Chofoza kuhlobo lwedivayisi ukuthola ulwazi oluthe xaxa kudivayisi ngayinye.

Iqoqwana Ivolumu flow Rate Amadivayisi ezinconyiwe
0.5 kuya ku-1.5mL na I-VialTweeter
1 kuya ku-500mL 10 kuya ku-200mL / min UP100H
10 kuya ku-2000mL 20 kuya ku-400mL / min UP200Ht, UP400S
0.1 kuya ku-20L 0.2 kuya ku-4L / min I-UIP1000hdT, I-UIP2000hdT
10 kuya ku-100L 2 kuya ku-10L / iminithi UIP4000
na 10 kuya ku-100L / iminithi UIP16000
na sikhulu yinhlanganisela UIP16000
I-ultrasonic irradiation ikhuthaza isizinda samakhemikhali asebenzayo kusuka ku-safron

I-Ultrasonicator I-UIP1000hdT for isizinda

Xhumana nathi! / Cela Us!

Sicela usebenzise ifomu elingezansi, uma ufisa ukucela ulwazi oluthe xaxa mayelana ne-homogenization yama-ultrasonic. Sizojabula ukukunikeza uhlelo lwe-ultrasonic ukuhlangabezana nezidingo zakho.









Sicela uqaphele wethu Inqubomgomo yobumfihlo.


Izincwadi / Izinkomba

I-Hielscher Ultrasonics yenza ama-homogenizer aphezulu we-ultrasonic wokuhlakazeka, i-emulsation kanye nokukhishwa kweseli.

Amandla aphezulu we-homogenizer ephezulu I-Lab kuya umshayeli futhi Izimboni isikali.