I-Hielscher Ultrasound Technology

Ultrasonic Bioreactors for Fermentation

  • I-Ultrasonics iyindlela ephumelelayo yokugqugquzela ama-microorganisms ngama-vibrations mechanical kanye ne-cavitation.
  • Ku-fermenter ye-sonobioreactor / ultrasonic, ukwelashwa kwe-ultrasonic yamangqamuzana nezicubu kuba yi-controllable kakhulu kusukela izimo zemvelo zingahle zizimisele.
  • With bioreactors ultrasonic, ukukhiqizwa ukukhiqizwa kungenziwa ngcono kakhulu.

Ukuvuthwa

Ukusebenza kahle kokuvutshelwa kuncike ezimweni zenkambiso: Ama-nutrients, ubukhulu bomphakathi, izinga lokushisa, okuqukethwe kwe-oxygen / gas kanye nokucindezelwa yizinto ezibalulekile ezithonya umsebenzi we-microbial. Ama-microorganisms kanye namaseli omamamalia akhula kuphela ngaphansi kwezimo ezithile. Izimo ezifanele zihlangene nokuvuselela kwe-ultrasonic kungenza ngcono isivuno sokuvutshelwa.

Ukukhuthazwa kwe-Ultrasonic yezinyunyana

Ukuvuthwa kuyindlela yokwenza umzimba eguqula ushukela kuma-acids, gesi, noma utshwala. Kwenzeka emvubelo namabhaktheriya, futhi nakwamaseli e-muscle alambile, njengasendleleni yokuvutshelwa kwe-lactic acid. Ukuvuthwa kusetshenziselwa kabanzi ngokubanzi ukubhekisela ekukhuleni okunamandla kwamagciwane ezincane ezikhulayo, ngokuvamile ngenhloso yokukhiqiza umkhiqizo othile wamakhemikhali.
Inqubo yokuvuthwa yenziwa ngezinga lezimboni ngokusebenzisa ama-microorganisms njengama-bacteria ne-fungus for the fermentation. Imikhiqizo etholakalayo isetshenziselwa ukudla kanye nomkhakha jikelele. Amakhemikhali, njenge-acetic acid, i-citric acid, ne-ethanol akhiqizwa ngokuvutshelwa. Izinga lokuvutshelwa lithonywa ukuhlushwa kwamagciwane ezincane, amaseli, izingxenye zamaselula, kanye nama-enzyme kanye nokushisa, kanye ne-pH. Ukuvutshelwa kwe-aerobic, i-oxygen iyisici esibalulekile futhi. Cishe zonke izinhlobo ze-enzyme ezikhiqizwa kwezohwebo, njenge-lipase, i-invertase kanye ne-rennet, zenziwe ngokuvutshelwa nge-microbes eziguquguqukayo.
Ngokuvamile, ukuvutshelwa kungahlukaniswa zibe izinhlobo ezine:

  • Ukukhiqizwa kwe-biomass (okubonakalayo okusebenzayo kweselula)
  • Ukukhiqizwa kwemetabolite ye-extracellular (amakhemikhali amakhemikhali)
  • Ukukhiqizwa kwezingxenye ze-intracellular (ama-enzyme namanye amaprotheni)
  • Ukuguqulwa kwe-substrate (lapho i-substrate eguqulwe yikhona umkhiqizo)

Sonication Ngaphambi, Ngesikhathi nangemva Fermentation

Ultrasonic Pre-treatment – Ukuthuthukiswa kokutholakala kwe-Biomass

I-ultrasonic yokuthambisa i-rice hull ukuthuthukisa i-hydrozysis enzymatic ye-xylooligosaccharides (XOs) ukukhiqizwa ngu-Aspergillus japonicus (var. Japonicus CY6-1). Ngo-sonication, ukukhiqizwa kwama-enzyme e-celylolytic ne-xylanolytic avela ekhanda likalayisi kwakhuliswa kakhulu. Isivuno se-hemicellulose sanyuka sibe yi-1.4-fold ngaphansi kwe-sonication kanye nesikhathi sokukhiqiza sancishiswa kakhulu kusuka 24 h kuya ku-1.5 h ngo-80ºC – kanye nokunye okusemandleni okusebenza kwenqubo. I-biomass ene-sonicated kulula ukuguqula i-fungi ukuze kuqinisekiswe umsebenzi we-enzyme futhi umsebenzi we-CMCase, b-glucosidase, ne-xylanase ukwandiswa uma uqhathaniswa nehotela elingenalo i-rice. Imikhiqizo yokugcina fermentative yayiyi-xylotetraose, i-xylohexaose, nesisindo esiphezulu se-molecular mass xylooligosaccharides. Isivuno se-xylohexaose esivela ku-helmese irayisi yayineminyaka engu-80 ephezulu.

Ultrasonic Fermentation – Ukukhuthazwa Kwezinto Ezincane

Sonication ngokulawula kahle futhi ephindaphindiwe kusiza ekuthuthukiseni ukukhiqizwa kwezinqubo ezahlukene zokuvutshelwa ngaphandle kokulimaza amaseli. Ukuqina kwe-sonication kungashintshwa ngqo ezinhlobonhlobo zezilwane nezidingo zayo. Nge-sonication elawulwayo, ukukhula kwamaseli kanye nokusetshenziselwa umzimba kunomthelela omuhle futhi ukuguqulwa okubanjwe ngamaseli abukhoma kuthuthukiswa kakhulu, isib. Ukugqugquzela i-Bifidobacteria ebisi.
Eminye izinqubo zokuvutshelwa kwe-fungal, i-sonication isetshenziswa ngokuphumelelayo ukuguqula ukukhula kwe-morphology kanye ne-broth rheology ngaphandle kokuphazamisa izinga lokukhula kanye nokukhiqizwa kwefungi le-filamentous.

I-Ultrasonic Post-treatment – Ukukhipha

Ukukhiqizwa kwezingxenye ze-intracellular ezifana ne-microbial enzymes (isib. Catalase, amylase, protease, pectinase, glucose isomerase, cellulase, hemicellulase, lipase, lactase, streptokinase) namaphrotheni aphindaphindiwe isici, i-streptokinase), amaseli kufanele ahlaziye / aphazamiseke ngemva kwenqubo yokuvuthwa ukukhulula amaprotheni ayifunayo. Nge-sonication, ukukhishwa kwe-intracellular kanye ne-extracellular polysaccharide-protein complexes ezivela ku-viscous mycelial fermentation mhluzi. Ngaphandle kokukhiqizwa kwayo okuvelele kanye nokusebenza kahle, i-sonication isungulwe futhi inokwethenjelwa kwe-cell lysis kanye nokukhishwa kwendaba ye-intracellular.
Chofoza lapha ukuze ufunde mor mayelana lysis ultrasonic kanye isizinda!

Ultrasonic Bioreactors

Hielscher Ultrasonics yisikhathi eside ehlangene nezinqubo ultrasonically ezikhuthaziwe. Sinikeza izigameko eziningi ze-ultrasonic ezihlukahlukene nobukhulu be-geometri. Ngaphandle kwalokho, sinikeza izixazululo ezenziwe ngokwezifiso zokuhlanganiswa ku-bioreactor yakho ekhona. Njengoba amaprosesa ethu e-ultrasonic ahlukahlukene kakhulu futhi adinga isikhala esilinganiselwe kuphela, ukuphindaphinda kwezitshalo ezikhona ze-biotechnological kungenziwa ngaphandle kwezinkinga.
Ithebula elingezansi, libonisa izincomo zedivayisi jikelele ngokuya kwevolumu ye-batch noma izinga lokugeleza okufanele lisetshenziswe. Chofoza kuhlobo lwedivayisi ukuthola ulwazi oluthe xaxa kudivayisi ngayinye.

Iqoqwana Ivolumu flow Rate Amadivayisi ezinconyiwe
0.5 kuya ku-1.5mL na I-VialTweeter
1 kuya ku-500mL 10 kuya ku-200mL / min UP100H
10 kuya ku-2000mL 20 kuya ku-400mL / min UP200Ht, UP400S
0.1 kuya ku-20L 0.2 kuya ku-4L / min I-UIP1000hdT, I-UIP2000hdT
10 kuya ku-100L 2 kuya ku-10L / iminithi UIP4000
na 10 kuya ku-100L / iminithi UIP16000
na sikhulu yinhlanganisela UIP16000
Izinqubo ze-Ultrasonic batch inikeza ukuxuba okusheshayo

Sonication of biomass

Isicelo solwazi




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I-ultrasonic irradiation ikhuthaza isizinda samakhemikhali asebenzayo kusuka ku-safron

I-Ultrasonicator UIP1000hd yesikhumba

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Sicela usebenzise ifomu elingezansi, uma ufisa ukucela ulwazi oluthe xaxa mayelana ne-homogenization yama-ultrasonic. Sizojabula ukukunikeza uhlelo lwe-ultrasonic ukuhlangabezana nezidingo zakho.









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