I-Hielscher Ultrasound Technology

Ultrasonic Anthocyanin Extraction

  • Ama-Anthocyanins asetshenziselwa kabanzi njengezakhi zemvelo nezomsoco emikhiqizweni yokudla.
  • Isizinda se-ultrasonic sithuthukisa ukukhululwa kwe-anthocyanins ephezulu kusuka ezitshalweni okuholela ekuvuneni okuphakeme kanye nenqubo esheshayo.
  • I-Sonication iyindlela elula, eluhlaza futhi ephumelelayo yokukhiqiza izimboni ze-anthocyanins yokudla / / i-pharma-grade grade.

Anthocyanins

Ama-Anthocyanins asetshenziselwa kabanzi njengamabalabala emvelo embonini yokudla. Zinezinhlobonhlobo eziningi zombala, ezivela e-orange ngokubomvu, ezibomvu nokuhlaza okwesibhakabhaka, kuye ngokuthi isakhiwo se-molecular kanye ne-pH value. Intshisekelo ku-anthocyanins ayikona kuphela ngokusekelwe emthonjeni wabo wokubala, kodwa futhi ngenxa yempahla yabo ezuzisa impilo. Ngenxa yokukhathazeka kwezemvelo nezempilo mayelana nokudayiswa kwamadayi, ama-dyes angokwemvelo angcono kakhulu njengoba umbala omnakekeli wemvelo wemboni yokudla nezidakamizwa.

Ukukhishwa kwe-Anthocyanin e-ultrasonically-ethuthukisiwe

Izinzuzo ze-Ultrasonic Extraction

  • Izithelo Eziphakeme
  • Inqubo yokukhipha ngokushesha – ngaphakathi kwamaminithi
  • Izitshalo eziphezulu zekhwalithi – isikhumba esincane, esingesona esishisayo
  • Ama-solvents ahlaza (amanzi, i-ethanol, i-glycerin, ama-vegetable amafutha njll)
  • Ukusebenza okulula futhi okuphephile
  • Ukutshalwa kwezimali okuphansi kanye nezindleko zokusebenza
  • Ukuzinza nokugcinwa okuphansi
  • Indlela eluhlaza, imvelo-friendly

I-UP100H nge-MS14 sonotrode yokukhishwa kwe-botanicals

Isicelo solwazi




Qaphela kwethu Inqubomgomo yobumfihlo.


Isizinda se-ultrasonic singenziwa ekusebenzeni kwe-batch nokuqhubeka kokugeleza-kwemodi. (Chofoza ukuze ukhulise!)

Ukusethwa kwe-Sonication nge I-UIP1000hdT ukukhishwa kwamakhemikhali e-bioactive kusuka ku-botanicals ku-batch. [Petigny et al. 2013]

Indlela yokukhipha ama-Anthocyyanins nge-Ultrasound? – Izifundo ze-Case

Ultrasonic Anthocyanin Extraction kusuka Rice Rice Oryza Sativa L.

Isizinda se-ultrasonic nge-UP200StIrayisi elibomvu lobunzima u-Oryza Sativa (owaziwa nangokuthi i-Violet Nori noma irayisi le-violet) ucebile ngokweqile kuma-phenolics njengeqembu le-favonoid le-anthocyanins. I-Turrini et al. (2018) wasebenzisa isizinda se-ultrasonic ukuze kuhlukaniswe ama-polyphenolic anjenge-anthocyanins kanye nama-antioxidants avela ku-caryopsis (ephelele, enobunundu, nefomu elenziwe nge-parboiled) namaqabunga elayisi elibomvu. Isizinda se-ultrasonic senziwa ngokusebenzisa i-Hielscher UP200St (200W, 26kHz, pic. kwesokunxele) ne-ethanol engu-60% njenge-solvent.
Ukuze kugcinwe ubuqotho be-anthocyanin, ukukhishwa kwe-ultrasonic kwagcinwa ku--20 ° C, okuvumela ukuwagcina okungenani izinyanga ezintathu.
I-Cyanidin-3 glucoside (eyaziwa nangokuthi i-chrysanthemin) yilapho i-anthocyanin enkulu ehlonishwayo e-'Volet Nori ',' i-Artemide 'ne-'Nerone' yezilimo zicwaningwa ekutadisheni kwe-Turrini et al., Kanti i-peonidin-3-glucoside ne-cyanidin- I-3-rutinoside (futhi i-antirrhinin) itholwe ngamanani aphansi.
Amaqabunga ama-violet e-Oryza Sativa angumthombo omuhle kakhulu we-anthocyanins kanye nokuqukethwe okuphelele kwe-phenolic (TPC). Ngesilinganiso cishe. I-2-3-iphakama ephakeme kunalawo elayisi nofulawa, amaqabunga e-Oryza ahlinzeka impahla engabizi kakhulu yokufakelwa kwe-anthocyanins. Umkhiqizo olinganisiwe we-4 kg we-anthocyanin / t amaqabunga amasha uphakeme kakhulu kunama-1 kg i-anthocyanin / t ilayisi, ebalwa ngesisekelo semali ephakathi kwe-anthocyanin etholakala 'e-Violet Nori' ilayisi (i-1300 μg / g ilayisi, njenge-cyanidin -3-glucoside) ukuze uthole isivuno esiphathelene no-68 kg welayisi kusuka ku-100 kg paddy.

Ultrasonic Anthocyanin Extraction kusuka Red Iklabishi

Ravanfar et al. (2015) baye baphenya ukusebenza kahle kwe-ultrasonic isizinda se-anthocyanins kusuka kwiklabishi ebomvu. Ukuhlolwa kwe-ultrasonic kwesizinda kwenziwa nge-ultrasonic system UP100H (Hielscher Ultrasonics, 30 kHz, 100 W). I-sonotrode MS10 (ububanzi be-10mm tip) ifakwe phakathi nendawo ye-glass beaker eyayilawulwa ngezinga lokushisa.
U-UP400St uvuselele ukusetha kwe-8L kokukhiphaIzindwangu zobomvu ezibomvu ezibomvu ezilinganiselwa ku-5mm ububanzi (isakhiwo se-cubic) kanye no-92.11 ± 0.45% wokuqukethwe kwamanzi okusetshenziselwa lokhu kuhlolwa. I-beaker ye-glass beaker (ivolumu: 200ml) yagcwala amanzi angu-100ml we-distilled kanye nezingcezu ezimbili ze-klabishi ebomvu. I-beaker yayihlanganiswe ne-aluminium foil ukuvimbela ukulahlekelwa kwe-solvent (amanzi) ngokukhuphuka ngesikhathi senqubo. Kuzo zonke izivivinyo izinga lokushisa ku-beaker lugcinwa ngokusebenzisa isilawuli se-thermostatic. Ama-sampuli aqoqwe ekugcineni, ahlungiwe futhi aphethwe yi-centrifuged ku-4000rpm kanti izidakamizwa zasebenziswa ukuze zithole ukukhiqizwa kwe-anthocyanin. Ukukhipha emanzini okugeza kwakwenziwa njengokuhlolwa kokulawula.
Umvuno omuhle we-anthocyanin kusuka kwiklabishi ebomvu wawunqunywe emandleni ka-100 W, isikhathi samaminithi angu-30 nokushisa kuka-15 ° C okwaholela ekuvuneni kwe-anthocyanin cishe ngo-21 mg / L.
Ngenxa yezinguquko zombala wayo kwi-pH value nombala wayo obala, ubisi obomvu iklabishi usetshenziswe njengendlela yokubonisa i-pH ekufakweni kwemithi noma njengama-antioxidants kanye namabalabala ezikhathini zokudla, ngokulandelana.

Isizinda se-Ultrasonic sikhuthaza ukukhululwa kwe-polyphenols njengama-anthocyanins avela ku-botanicals.

I-Ultrasonics iqinisa isisindo se-anthocyanins esakhiweni sezitshalo ngokuphawulekayo.
umthombo: Ravanfar et al. 2015

Olunye ucwaningo lubonisa isizinda se-ultrasonic esiphumelele se-anthocyanins kusuka ku-blueberries, ama-blackberries, amagilebhisi, ama-cherries, ama-strawberries, nama-sweet potato phakathi komunye.

I-Hielscher Ultrasonics ikhiqiza ama-ultrasonicators aphezulu ekusebenzeni kwezicelo ze-sonochemical.

Amaprosesa aphezulu aphezulu avela ku-ultrasonic I-Lab ukushayela futhi izimboni zezimboni.

Ukusebenza okuphezulu kwe-Ultrasonic Extractors

Ultrasonic Process Testing And AnalysisI-Hielscher Ultrasonics ikhethekile ekukhiqizeni amaphrosesa aphezulu asebenzayo e-ultrasonic ekukhiqizeni izithelo eziphezulu ezivela ku-botanicals.
Iphothifoliyo yomkhiqizo omkhulu kaHielscher isukela kuma-lab ultrasonicators amancane, anamandla amakhulu kumabhentshi amakhulu kanye nezinhlelo ezigcwele zezimboni, eziletha ukuphakama okukhulu kwe-ultrasound ukuze kusetshenziswe kahle futhi kuhlukaniswe izinto eziphilayo (isib. I-anthocyanins, i-gingerol, Ipiperine, i-curcumin njll). Wonke amadivayisi we-ultrasonic avela 200W kuya 16,000W kufaka isibonisi ezinemibala yokulawula idijithali, ikhadi elihlanganisiwe le-SD lokuqopha okuzenzakalelayo kwedatha, ukulawula okukude kwesiphequluli kanye nezinye izici eziningi zomsebenzisi. I-sonotrodes namaseli okugeleza (izingxenye, ezithintana nephakathi) zingenziwa nge-autoclaved futhi kulula ukuzihlanza.
Ama-processor ultrasonic aseHielscher asetshenziselwa umsebenzi we-24/7 ngaphansi komthwalo ogcwele, adinga ukugcinwa okuphansi futhi kulula futhi ephephile ukusebenza. Ukubonisa umbala wedijithali kuvumela ukulawulwa komsebenzisi-friendly we-ultrasonicator.
Amasistimu ethu akwazi ukuletha kusuka phansi kuya kuma-amplitudes aphakeme kakhulu. Ukukhishwa kwe-cannabinoids ne-terpenes, sinikeza i-sonotrodes ekhethekile ye-ultrasonic (eyaziwa ngokuthi i-ultrasonic probes noma izimpondo) ezilungiselelwe ukuhlukaniswa okunengqondo kwezinto eziphezulu ezisebenzayo. Zonke izinhlelo zethu zingasetshenziselwa ukukhipha futhi emva kwalokho ku-emulsification yamagciwane. Ukuqina kwemishini ye-ultrasonic ye-Hielscher kuvumela ukusebenza okuqhubekayo (24/7) emsebenzini obanzi futhi ezindaweni ezifuna izinto.

Ukulawulwa okuqondile kwemigomo yezinqubo ze-ultrasonic kuqinisekisa ukukhiqizwa kabusha nokucubungula ukulinganisa.
Ithebula elingezansi linikeza isibonakaliso somthamo wokucubungula we-ultrasonicators wethu:

Iqoqwana Ivolumu flow Rate Amadivayisi ezinconyiwe
1 kuya ku-500mL 10 kuya ku-200mL / min UP100H
10 kuya ku-2000mL 20 kuya ku-400mL / min UP200Ht, UP400St
0.1 kuya ku-20L 0.2 kuya ku-4L / min I-UIP2000hdT
10 kuya ku-100L 2 kuya ku-10L / iminithi I-UIP4000hdT
na 10 kuya ku-100L / iminithi UIP16000
na sikhulu yinhlanganisela UIP16000

Xhumana nathi! / Cela Us!

Cela ulwazi oluthe xaxa

Sicela usebenzise ifomu elingezansi, uma ufisa ukucela ulwazi oluthe xaxa mayelana ne-homogenization yama-ultrasonic. Sizojabula ukukunikeza uhlelo lwe-ultrasonic ukuhlangabezana nezidingo zakho.









Sicela uqaphele wethu Inqubomgomo yobumfihlo.


Uhlelo lwe-Ultrasonic lokufakelwa u-UIP4000hdT

I-UIP4000hdT (4kW) iprosesa ye-ultrasonic yesikhumba

Izincwadi / Izinkomba

  • Chemat, Farid; Rombaut, Natacha; Sicaire, Anne-Gaëlle; I-Meullemiestre, u-Alice; UFabiano-Tixier, u-Anne-Sylvie; U-Abert-Vian, uMaryline (2017): Isizinda se-ultrasound esisiziwe sokudla nemikhiqizo yemvelo. Izindlela, amasu, izinhlanganisela, izivumelwano nezinhlelo zokusebenza. Ukubuyekezwa. Ultrasonics Sonochemistry 34 (2017) 540-560.
  • Ravanfar, uRaheli; UTamadon, Ali Mohammad, Niakousari, Mehrdad (2015): Ukulungiswa kwe-ultrasound isizinda sokusizwa kwe-anthocyanins kusuka kwiklabishi ebomvu usebenzisa indlela ye-Taguchi design. J Food Sci Technol. 2015 Dec; 52 (12): 8140-8147.
  • I-Turrini, i-Federica; I-Boggia, i-Raffaella; ULeardi, uRiccardo; Borriello, Matilde; I-Zunin, i-Paola (2018): Ukulungiswa kwe-Ultrasonic-Assisted Extraction yama-Phenolic Compounds ophuma ku-Oryza Sativa L. 'Violet Nori' nokunqunywa kwezindawo zokuvikela i-Caryopses namahlamvu. Ama-molecule 2018, 23, 844.


Amaqiniso Okufanele Ukwazi

Umsebenzi Wokwenzwa Ngokwenziwa Nge-Ultrasound?

Ukusetshenziswa kwamagagasi amakhulu e-ultrasound kuya emithonjeni yamanzi ephuma ku-cavitation. Isimo se cavitation iholela endaweni ekushisa okushisa kakhulu, izingcindezi, izinga lokushisa / ukupholisa, ukuhluka kwezingcindezi kanye namandla aphezulu e-shear emaphakathi. Lapho ama-bubbles cavitation efakaza phezu kwamanzi amaningi (njengezinhlayiya, ama-cell plant, ama-tissue njll), ama-micro-jets kanye nokushayisana kwe-interparticlular kubangela imiphumela efana nokucubungula ubuso, ukuguguleka kwamanzi kanye nokuphazamiseka kwezinhlayiyana. Ukwengeza, implosion ye-bubble cavitation emithonjeni yamanzi idala ama-macro-turbulences kanye nokuxuba okuncane.
Ukuchithwa kwe-Ultrasonic yezinto ezitshalayo zitshalo kuhlukanisa i-matrix yamaseli ezitshalo futhi kuthuthukisa i-hydration efanayo. I-Chemat et al (2015) iphetha ngokuthi isizinda se-ultrasonic samakhemikhali e-bioactive avela ku-botanicals siwumphumela wezici ezihlukene ezizimele noma ezihlangene ezihlanganisa ukuhlukaniswa, ukuguguleka kwamandla, ukukhwabanisa, ukuthunyelwa kwe-detexturation nokudumisa. Lezi zinkinga ziphazamisa udonga lwamaseli, zithuthukisa ukudluliswa kwamandla ngokususa i-solvent esitokisini nokuncelisa i-phyto-compound elayishiwe, futhi uqinisekise ukunyakaza kwamanzi ngokuxuba okuncane.

I-ultrasonic / acoustic cavitation idala amabutho amakhulu kakhulu avula izindonga zeseli eziziwa ngokuthi i-lysis (Chofoza ukuze ukhulise!)

Isizinda se-ultrasonic sisekelwe ku-cavitation yama-acoustic namandla ayo we-hydrodynamic shear

Ukuchithwa kwe-Ultrasonic yezinto ezitshalayo zitshalo kuhlukanisa i-matrix yamaseli ezitshalo futhi kuthuthukisa i-hydration efanayo. Chemat et al. (2015) iphetha ngokuthi isizinda se-ultrasonic se-bioactive compounds kusuka ku-botanicals siwumphumela wezici ezihlukene ezizimele noma ezihlangene ezihlanganisa ukuhlukaniswa, ukuguguleka kwamandla, ukukhishwa kwe-capillarity, i-detexturation ne-sonoporation. Lezi zinkinga ziphazamisa udonga lwamaseli, zithuthukisa ukudluliswa kwamandla ngokususa i-solvent esitokisini nokuncelisa i-phyto-compound elayishiwe, futhi uqinisekise ukunyakaza kwamanzi ngokuxuba okuncane.
Isizinda se-ultrasonic siphumelela ukuhlukaniswa okusheshayo kakhulu kwamakhemikhali - ukuphelelwa yisikhathi kwezindlela ezijwayelekile zokukhipha esikhathini sokucubungula isikhathi esifushane, isivuno esiphezulu, nasezingeni eliphansi lokushisa. Njengendlela yokwelashwa okunomsoco, i-ultrasound-eyasizwa isizinda igwema ukuchithwa okushisayo kwezingxenye eziphilayo eziphakemeyo nokuqhathanisa namanye amasu afana ne-conventional solvent extraction, i-hydrodistillation, noma isizinda soSoxhlet, esaziwa ukubhubhisa amamolekli azwela ukushisa. Ngenxa yalezi zinzuzo, isizinda se-ultrasonic yindlela yokukhetha yokukhululwa kwama-bioactive okushisa okushisa okuvela kubhodini.

Iziphazamisi ze-Ultrasonic zisetshenziselwa ukuchithwa okuvela emithonjeni ye-phyto (isb. Izitshalo, i-algae, isikhunta)

Isizinda se-Ultrasonic esivela kumaseli esitshalo: isigaba esincane esithintekayo (TS) sibonisa indlela yokwenza phakathi kwesikhumba se ultrasonic kusuka kumangqamuzana (ukukhulisa 2000x) [isisetshenziswa: Vilkhu et al. 2011]

Anthocyanin – I-pigment ebalulekile yokutshala

Ama-Anthocyanins angama-pigment plant, angabonakala abomvu, obomvu, obuluhlaza noma omnyama. Ukuboniswa kombala wezingulube ze-anthocyanin ezincibilika kwamanzi kuxhomeke ekubalukeni kwe-pH. Ama-Anthocyanins atholakala e-cell vacuole, ikakhulukazi ezihlotsheni nezithelo, kodwa futhi emaqabunga, iziqu kanye nezimpande, lapho zitholakala khona ikakhulu ezingxenyeni zangaphandle zamaseli ezifana namaseli e-epidermis ne-peripheral mesophyll.
Okuvamile okwenzeka njalo emvelweni yi-glycosides ye-cyanidin, i-delphinidin, i-malvidin, i-pelargonidin, i-peonidin, ne-petunidin.
Izibonelo ezinhle zezitshalo ezicebile ku-anthocyanins zifaka izinhlobo ze-vaccinium, njenge-blueberry, i-cranberry, ne-bilberry; Ama-rubus amajikijolo, afaka phakathi okusajingijolo okumnyama, okusajingijolo okubomvu, namabhijijolo; i-blackcurrant, i-cherry, isitshalo seqanda, irayisi elimnyama, ube, ama-potato ama-Okinawan, i-concord yamagilebhisi, i-muscadine yamagilebhisi, iklabishi ebomvu namagundane e-violet. Ama-peaches abomvu aphuzile nama-apula aqukethe ama-anthocyanins. I-Anthocyanins ayiningi kakhulu ebhanini, i-asparagus, i-pea, i-fennel, i-pear, namazambane, futhi kungenzeka ukuthi ayisekho ngokuphelele emasimini athile ama-gooseberries aluhlaza.

Ama-Anthocyanins afana ne-cyanidin, i-delphinidin, i-pelargonidin, i-peonidin, i-malvidin ne-petunidin ingahle isetshenziswe ngokuphumelelayo ngokusebenzisa amandla we-ultrasound.

Ukwakhiwa kwe-anthocyanins eyinhloko

Ama-Anthocyanins ayindlela enhle yokufaka esikhundleni sokufaka imibala kwama-synthetic agents kumikhiqizo yokudla. Ama-Anthocyanins avunyelwe ukusetshenziswa njengama-colorants wokudla e-European Union, e-Australia naseNew Zealand, enekhodi enemibala engu-E163. Ama-Anthocyanins atholakala kwizithelo nemifino futhi angachazwa njengohlobo lwamakhemikhali wezitshalo ancibilikiswa amanzi. Amakhemikhali, ama-anthocyanins ama-glycosides ama-anthocyanidin asuselwa esakhiweni se-2-phenylbenzophyrylium (flavylium). Kunama-phytochemicals angaphezu kwama-200 ahlukile awela esigabeni sama-anthocyanins. Njengombala ophambili wezithelo ezithelo zasendle namajikijolo, kunemithombo eminingi lapho kukhishwa ama-anthocyanins. Umthombo ovelele wama-anthocyanins yisikhumba samagilebhisi. Imisebe ye-anthocyanin esikhunjeni samagilebhisi ifaka ikakhulu i-di-glucosides, i-mono-glucoside, ama-monoglucosides acylated kanye ne-acylated di-glucosides ka-peonidin, i-malvidin, i-cyanidin, i-petunidin ne-Delphinidin. Okuqukethwe kwe-anthocyanin kumagilebhisi kuyahlukahluka kusuka ku-30-750mg / 100g.
Ama-anthocyanins avelele kakhulu yi-cyanidin, delphinidin, pelargonidin, peonidin, malvidin ne-petunidin.
Isibonelo i-anthocyanins peonidin-3-caffeoyl-p-hydroxybenzoyl sophoroside-5-glucoside, peonidin-3- (6 ″ -caffeoyl-6 ‴ -feruloyl sophoroside) -5-glucoside, ne-cyanidin-3-caffeoyl-p-hydroxyben i-sophoroside-5-glucoside itholakala kumazambane amnandi onsomi.

Anthocyanins – izinzuzo zezempilo

Ngaphandle kwamandla abo amakhulu wokusebenza njengombala wokudla wemvelo, ama-anthocyanins abaluleke kakhulu ngemiphumela yawo ye-antioxidative. Ngakho-ke, ama-anthocyanins akhombisa imiphumela eminingi emihle yezempilo. Ucwaningo luye lwabonisa ukuthi ama-anthocyanins angavimbela ukulimala kwe-DNA kumaseli womdlavuza, anqande ama-enzymes wokugaya, afaka ukukhiqizwa kwe-insulin emangqamuzaneni angawodwa pancreatic, anciphise izimpendulo zokuvuvukala, avikele ekunciphiseni okuhlobene nobudala bomsebenzi wobuchopho, athuthukise ukuqina kwemithambo yegazi ye-capillary futhi avikele ukuhlangana kwe-thrombocyte.