I-Hielscher Ultrasound Technology

I-Superior Catechin Extracts ene-Ultrasonics

I-Catechin efana ne-epigallocatechin gallate (EGCG), i-epigallocatechin (EGC), i-epicatechin gallate (ECG), ne-epratechin (EC) ngama-polyphenols, abonisa izinzuzo ezinkulu zezempilo. Ngenkathi i-catechin itholakala ngamanani amancane etiyeni eliluhlaza okotshani, cocoa, izithelo nakokunye ukudla, ngoba kunconywa umthamo omkhulu wokukhishwa kwe-catechin. Ukukhishwa kwe-Ultrasonic kuyindlela esebenzayo futhi esheshayo eluhlaza yokuhlukanisa ama-catechins ezitshalweni, isib. Amaqabunga e-green green, kusetshenziswa amanzi njenge-solvent. Okukhishwa kwe-catechin okukhiqizwa yi-Ultrasonically kungamakhwalithi aphezulu aphezulu, angaqediwe ukusetshenziswa kwezithako zokudla okunempilo kanye nemithi.

Isizinda se-Ultrasonic sama Catechins

Amacembe wetiye aluhlaza acebile ngama-catechin afana ne-epigallocatechin gallate (EGCG), epigallocatechin (EGC), epicatechin gallate (ECG), ne-epicatechin (EC), engakhishwa yi-ultrasonication.Ukukhishwa kwe-Ultrasonic kuyindlela engashisiyo, esekelwe ekusebenziseni amandla amsulwa emishini. Imvamisa ephansi, amagagasi aphezulu e-ultrasound ahlanganiswa nge-probe ye-ultrasonic (sonotrode) endaweni ephakathi noketshezi. Amandla amakhulu e-shear we-acoustic cavitation perforate futhi aphazamisa ulwelwesi lweseli, athuthukisa ukudluliselwa kwesisindo phakathi kokuphakathi kweseli kanye nogwebu, futhi akhulule amakhompiyutha angaphakathi kwe-solvent.
Ekubukezeni kwabo kabanzi kwezindlela zokukhipha ethafeni eliluhlaza okotshani (iCamellia sinensis), iBanerjee neChatterjee (2015) bathola ukuthi abasebenza nge-probe-hlobo ultrasonicators basebenza kahle kakhulu kunokugeza okuphezulu kwe-ultrasonic. Ngaphezu kwalokho, ababhali bathi ukukhipha i-ultrasonic kuyindlela ekhethwayo yokukhishwa kwetiye lekati ngenxa yokukhuphuka kokusebenza kwenqubo yokufikisa emazingeni okushisa aphansi, okuvimbela ukungcoliswa okushisa komzimba kwezakhi ezibucayi bokushisa futhi kulondoloze inani labo lokwelashwa. Ukukhishwa okushisa okuphezulu kuvame ukuholela ekuwohlokeni kwama-polyphenols futhi kukhuphule ukukhishwa kwamaprotheni ne-pectin, okuphazamisa ikhwalithi ye-organoleptic yetiyi ngokwakhiwa kukakhilimu. Inzuzo ye-sonication ilele kumshini wayo ongashisi kakhulu. Ukukhishwa kwe-Ultrasonic kungakhuphula isivuno se-polyphenol yetiye kuma-65 ° C ngenkathi kuqhathaniswa nokukhipha okungokwesiko ku-85 ° C.
Ukukhishwa kwe-Ultrasonic kunikeza isivuno esiphezulu sezakhi ezisezingeni eliphezulu. Njengenqubo engeyona eyokushisa, ukukhishwa kwe-ultrasonic kugwema ukulahleka kwezakhi ezithinta ukushisa.
Ukukhishwa kwe-Ultrasonic nakho kungenziwa ngokushisa kwamakamelo noma koketshezi olupholile. Chofoza lapha, ukuze ufunde kabanzi mayelana netiye elibandayo elibandayo le-ultrasonic!

Izinzuzo ze-Ultrasonic EGCG Extraction

  • I-solvent-free / based-ngamanzi
  • ukukhiqizwa okuphezulu
  • Izitshalo eziphezulu zekhwalithi
  • I-Spectrum egcwele Extracts
  • inqubo esheshayo
  • Luhlaza okotshani, okwezemvelo
  • operation elula futhi ephephile
  • Ukugcinwa okuphansi
  • i-RoI esheshayo
Iprosesa le-Ultrasonic UIP2000hdT (2kW) elinamandla ashukumisayo we-batch

I-homogenizer ye-ultrasonic I-UIP2000hdT (2kW) ene-batch reactionor eqhubekayo

Isicelo solwazi




Qaphela kwethu Inqubomgomo yobumfihlo.


Case Study of Ultrasonic Catechin Isizinda

Ukukhishwa kwe-Ultrasonic kungenziwa kusetshenziswa i-solvents ehlukahlukene, kufaka phakathi amanzi, i-ethanol, amanzi: ukuxubana kwe-ethanol, isopropanol, uwoyela wemifino, i-glycerine, njll.
Esifundweni esivela ku-2018, u-Ayyildiz et al. kuqhathaniswa ukukhishwa kwe-ultrasonic kusetshenziswa amanzi ne-ethanol njengezinyibilikisi nangendlela evamile yokukhishwa kwamanzi ashisayo. Kulolu cwaningo ngezinga lokushayela, abasebenzisa a I-Hielscher UIP2000hd (2kW, 20kHz) I-ultrasonicator ku-batch nasekusethweni okuqhubekayo kokugeleza.
Imiphumela yabonisa ukuthi isizinda se-ultrasonic nge-ethanol saphawuleka (k < 0.05) more efficient to extract higher yields of EGCG, EGC, ECG, and EC than conventional hot water extraction and ultrasonic water-based extraction. Under optimised process conditions, almost 100% and 50% more EGCG content was obtained ultrasonic ethanol extraction than conventional hot water extraction and ultrasonic extraction with water, respectively. The optimal conditions for the ultrasonic extraction of EGCG with ethanol were 66.53ºC, 43.75 min and, 67.81% ethanol. Ultrasonic extraction is the preferred mode of tea catechins due to the increased efficacy of extraction process at lower temperature by retaining their antioxidant activity. [/two_thirds] [one_third_last] [/one_third_last] [two_thirds]

Ukusebenza okuphezulu kwe-Ultrasonic Extractors

Izinhlelo zokukhishwa kwe-Hielscher Ultrasonics 'zisetshenziswa emhlabeni wonke ekudleni nasapharma ekukhiqizweni kwezentengiso okwenziwa kwezinto ezisezingeni eliphezulu ezisetshenziswa njengezithako zokudla nemithi yemithi. Uma ufuna ukukhiqiza amabhathi amancane wetiye elibandayo noma wenza inqubo enkulu ye-polyphenol / catechin extracts ephezulu, iHielscher Ultrasonics inesithombisi esifanelekile se-ultrasonic. Ama-ultrasonicators alula futhi aphephile ukusebenza. Isoftware enembile kanye nokulawulwa kwedijithali nge-touch-ukubonisa kuvumela ukulawula inqubo okuqondile. Ukuba namandla kwemishini yokusebenza kwe-Hielscher sika-Hielscher kuvumela ukusebenza kwe-24/7 emsebenzini osindayo nasezindaweni ezifunwayo.

Inqubo yokumiswa ngokuphelele kwe-Hielscher Ultrasonics

Izinto zokukhishwa zokudla- kanye neze-pharma-grade kufanele zikhiqizwe ngokuya nge-Okuhle Ukukhiqiza Okusebenzayo (i-GMP) nangaphansi kokucaciswa kokusebenza okuhlelekile. Izinhlelo ze-Hielscher Ultrasonics 'zokukhishwa kwedijithali ziza ne-software ehlakaniphile, eyenza kube lula ukusetha nokulawula inqubo ye-sonication ngokunembile. Ukuqoshwa kwedatha ye-othomathikhi kubhala yonke imingcele yenqubo ye-ultrasonic efana namandla e-ultrasound (inani eliphelele namandla e-net), amplitude, izinga lokushisa, ingcindezi (lapho izinzwa ze-temp kanye nezingcindezi zifakwa) ngedethi nesikhathi nesikhathi esakhelwe ku-SD-khadi. Lokhu kukuvumela ukuthi ubuyekeze kabusha inkatho ngayinye eyenziwe ngobunyoninco. Ngasikhathi sinye, ukuqinisekiswa kabusha kwekhwalithi ephezulu yomkhiqizo kuyaqinisekiswa.
Ithebula elingezansi linikeza isibonakaliso somthamo wokucubungula we-ultrasonicators wethu:

Iqoqwana Ivolumu flow Rate Amadivayisi ezinconyiwe
1 kuya ku-500mL 10 kuya ku-200mL / min UP100H
10 kuya ku-2000mL 20 kuya ku-400mL / min UP200Ht, UP400St
0.1 kuya ku-20L 0.2 kuya ku-4L / min I-UIP2000hdT
10 kuya ku-100L 2 kuya ku-10L / iminithi I-UIP4000hdT
na 10 kuya ku-100L / iminithi UIP16000
na sikhulu yinhlanganisela UIP16000

Xhumana nathi! / Cela Us!

Cela ulwazi oluthe xaxa

Uyacelwa ukuthi usebenzise ifomu elingezansi ukucela ulwazi olungeziwe mayelana nezinhlelo zokusebenza ze-ultrasonic, izinhlelo zokusebenza nentengo. Sizokujabulela ukuxoxa ngenqubo yakho nawe futhi sikunikeze uhlelo lwe-ultrasonic oluhlangabezana nezidingo zakho!









Sicela uqaphele wethu Inqubomgomo yobumfihlo.


I-Hielscher Ultrasonics yenza ama-homogenizer aphezulu we-ultrasonic wokuhlakazeka, i-emulsation kanye nokukhishwa kweseli.

Amandla aphezulu we-homogenizer ephezulu I-Lab kuya umshayeli futhi Izimboni isikali.

Izincwadi / Izinkomba



Amaqiniso Okufanele Ukwazi

Isizinda se-Ultrasonic – isimiso sokusebenza

Ukukhishwa kwe-Ultrasonic kuyindlela enkulumelayo yokukhulula futhi ihlukanise i-intracellular compound kusuka kumatrices weseli njengamaseli wesitshalo. Lapho amagagasi anamandla aphezulu e-ultrasound ehlanganiswa abe yi-slurry (isb. Enezinhlayiya zezitshalo ezinamacici amaningi emanzini noma i-solvent), amaza amakhulu we-ultrasound akhiqiza i-cavitation. Umcimbi we-cavitation uholela endaweni yangakithi emazingeni okushisa ngokweqile, izingcindezi, amazinga okushisa / ukupholisa, umehluko wokucindezela namandla amadevu aphezulu phakathi. Lapho ama-bubble we-cavitation efaka ebusweni bezinto ezinamandla (ezinjengezinhlayiya, amaseli ezitshalo, izicubu njll.), Ama-jets amancane nokushayisana kwe-inclarticlular kukhiqiza imiphumela efana nokubhoboza komhlaba, ukuguguleka kanye nokuqhekeka kwezinhlayiyana. Ngaphezu kwalokho, ukuvezwa kwama-bubble we-cavitation kwimidiya ye-liquid kudala ukuxubana kwama-macro-turtle kanye nokuxubana okuncane.
I-Ultrasonic irradiation imele indlela ephumelelayo yokuthuthukisa izinqubo zokudlulisa ngobuningi, ngoba i-sonication iphumela emgudwini nezinqubo zayo ezihlobene njengokuhamba kwe-micros-by jets liquid, compression and decompression in the material with a following following wall of cell wall, and heating high and amazinga okupholisa.

Iziphazamisi ze-Ultrasonic zisetshenziselwa ukuchithwa okuvela emithonjeni ye-phyto (isb. Izitshalo, i-algae, isikhunta)

Isizinda se-Ultrasonic esivela kumaseli esitshalo: isigaba esincane esithintekayo (TS) sibonisa indlela yokwenza phakathi kwesikhumba se ultrasonic kusuka kumangqamuzana (ukukhulisa 2000x) [isisetshenziswa: Vilkhu et al. 2011]

Ultrasonication wezitshalo izingcezwana matrix yamaseli wezitshalo futhi kuthuthukisa i-hydration efanayo. Cmat et al. (2015) uphetha ngokuthi ukukhishwa kwe-ultrasonic kwezakhi ze-bioactive kusuka ku-botanicals kungumphumela wezindlela ezihlukene ezizimele noma ezihlanganisiwe kufaka phakathi ukuhlukaniswa, ukuguguleka, ukuqina, ukuphindaphindeka, kanye ne-sonoporation. Le miphumela iphazamisa udonga lweseli, ithuthukise ukuhanjiswa kwamasokisi amaningi ngokucindezela i-solvent esitokisini futhi incela i-phyto-composed yokulayisha i-phyto-composed, futhi iqinisekise ukuhamba kwe-liquid ngokuxubana okuncane.

Yimiphi Izinzuzo Ezikhulu Zokukhipha i-Ultrasonic?

  • Inqubo esheshayo nokukhipha okuphezulu
  • Ukusebenzisa Amandla Ephansi
  • Izindleko zokunciphisa ezincishisiwe
  • Ubuchwepheshe obungeyona eyokushisa
  • Ubumsulwa obuphakeme
  • Ubuchwepheshe obuluhlaza

I-Acoustic Cavitation kanye nemiphumela yayo

Ku-ketshezi, amaza we-ultrasound adala imijikelezo eshintshashintshashintshashintshashintshashintshashintshintshintshintshintshintshintshisi eshintshashintshintshintshashintshintshintshintshintshintshintshintshintshintshintshintshintshintsaphoinji / okukhulu, okuholela ekwakhekeni kwamabhamuza we-cavitation. Phezu kwemijikelezo eminingana yokucindezela, ama-bubble we-cavitation akhula aze afinyelele umkhawulo, lapho i-bubble ingakwazi ukuthola khona amandla amaningi. Ngalesi sikhathi i-bubble ifakwa ngobudlova. Ngenkathi i-bubble implosion izimo ezibucayi ezifana nokushisa okuphezulu okufika ku-5000K, izingcindezi ezifinyelela ku-2000atm, amazinga okushisa aphakeme kakhulu / okupholisa kakhulu nokwehluka kwengcindezi. Njengoba amandla e-bubble fall fall ashesha kunokudluliselwa ngobuningi nokushisa, amandla asemgodini oshayayo abekelwe indawo encane kakhulu, ebizwa nangokuthi "indawo eshisayo". Ukufakwa kwe-bubble ye-cavitation kubuye kube nemiphumela emincane yama-microturbulefere, ama-jets we-liquid afinyelela ku-280m / s velocity kanye nomphumela wamabutho e-shear. Le nto yaziwa ngokuthi i-ultrasonic noma i-acoustic cavitation.

I-ultrasonic / acoustic cavitation idala amabutho amakhulu kakhulu avula izindonga zeseli eziziwa ngokuthi i-lysis (Chofoza ukuze ukhulise!)

Isizinda se-ultrasonic sisekelwe ku-cavitation yama-acoustic namandla ayo we-hydrodynamic shear

Amakhekhe

Itiye eliluhlaza laziwa ngokuceba ngama-polyphenols anjenge-caffeic acid, i-gallic acid, i-catechin, i-eparrchin, i-gallocatechin, i-catechin gallate, i-gallocatechin gallate, i-epicatechin gallate, i-epigallocatechin, ne-epigallocatechin gallate (EGCG). isiphuzo kanye nokukhipha. I-EGCG yi-catechin eyaziwayo, etholakala ngamanani aphezulu emacembe omisiwe wetiyi eluhlaza (7380 mg nge-100 g), itiye elimhlophe (4245 mg nge-100 g), futhi ngamanani amancane etiyeni elimnyama (936 mg nge 100 g). Ngesikhathi sokukhiqizwa itiye elimnyama, ama-catechins aguqulwa kakhulu kuma-theaflavins nama-thearubigins nge-polyphenol oxidases.

Izinzuzo Zempilo ze-Epigallocatechin Gallate (EGCG)

Eqenjini lama-catechins, i-epigallocatechin gallate (EGCG) yiyona ecwaningwe kakhulu futhi ethembisa kakhulu. Ukusuka ekulweni nomdlavuza, i-antioxidative, anti-inflammatory, anti-fibrosis, anti-collagenase yokuvuselela amasosha omzimba kanye nemiphumela yokulwa nokuguga, i-EGCG ikhombisa izinzuzo eziningi ngakho-ke ichithwa ngendlela yesiphuzo setiye eluhlaza kanye nangendlela yokudla njengamaphilisi, izimpuphu, amaphilisi njll.
Ucwaningo luphawula ukuthi ama-catechin afana ne-epigallocatechin gallate (EGCG) anganciphisa ukuvuvukala futhi angavimbela izimo ezithile ezingamahlalakhona, njengesifo senhliziyo, isifo sikashukela, kanye nezinye izinhlobo zomdlavuza.

I-EGCG nemiphumela yako ye-Anticancer

Njengoba umdlavuza uyisifo esivame ukusongela impilo, izakhiwo ze-anticancer ze-EGCG zingaphansi kocwaningo olunzima. Ucwaningo lukhombisa ukuthi i-EGCG ingavimba i-tumorigenesis ngokunciphisa noma ngokuqeda imiphumela ye-carcinogen. Kukhona okutholayo kusikisela ukuthi i-EGCG ivimbela i-diethylnitrosamine-ikiwaxise ukukhuluphala okuhlobene nesibindi ngokuvimbela i-axF ye-IGF / IGF-1R, ukuthuthukisa i-hyperinsulinemia, kanye nokuthola ukuvuvukala okungamahlalakhona. Enye indlela yokusebenza kwe-anticancer ye-EGCG ukuvimbela i-angiogeneis futhi ngaleyo ndlela kuvimbele ukwanda kwesimila.

I-EGCG nemiphumela yayo ye-antioxidative

Izinqubo ezingenakubalwa ze-antioxidative zenzeka emzimbeni womuntu futhi ziba nomthelela kwezempilo, amandla nenhlala-kahle. I-EGCG yi-antioxidant. Ama-antioxidants angavikela ekulimaleni kwamaselula ngokuqothula u-radicals wamahhala nangokuwahlukanisa. I-phenol ikhala esakhiweni se-EGCG njengezicupho ze-elektroni kanye nemikhonto yama-radical yamahhala, ivimbela ukwakheka kwezinhlobo ze-oksijini ezisebenzayo, futhi inciphise umonakalo odalwe ukucindezelwa kwe-oxidative.

I-EGCG kanye nemiphumela yayo yokulwa nokuvuvukala

Ukuvuvukala kungabangelwa izifo, ukucindezela okungapheli kanye nokungcoliswa kwemvelo. Umzimba ufinyelela kulezi zinto zokucindezela ngokuvuvukala, okubonakala ngokuhlanganiswa kwenqwaba yamaseli omzimba ezindaweni zokuvuvukala, ukukhishwa kwama-cytokines we-proinfigueatory, kanye nezinhlobo ze-oksijini / nitrogen (ROS / RNS). I-ROS / RNS ihlobene nokwenza kusebenze kwe-transcript factor NF-B ne-activator protein- (AP-) 1. Ngemuva kokuhlinzwa, i-NF-jB kanye ne-AP-1 yokudluliselwa kusuka ku-cytoplasm iye ku-nucleus futhi kukhuphule izinkulumo ezahlukahlukene zokuvuvukala, okuthi ngemuva kwalokho kubange ukuphendula okulimazayo kokulimala nokulimala kwezicubu.
I-EGCG ivimbela ukudluliselwa kwe-NF-B ne-AP-1 ukuthi isichasiso se-iNOS ne-COX-2 sibekelwa phansi ikakhulukazi ngokuqothula i-nitric oxide, i-peroxynitrite, nezinye i-ROS / RNS nokukhiqizwa kwezimpawu zokuvuvukala kuncishisiwe.

I-EGCG kanye Nemiphumela Yakho Kokukhuthaza i-Osteogenesis

I-Osteoporosis yisifo esibi esibonakaliswa ukuwohloka kwe-matrix yethambo kanye nokulahleka kokuqina kwamathambo. I-EGCG ikhombisa imiphumela yokulawula kumathambo wethambo. I-EGCG ingayenga i-apoptosis yama-ococlasts futhi ivimbele ukwakheka kwamathambo ngokuvimba isizukulwane se-NF-B ne-IL-1b. Ngaphezu kwalokho, ingakhuthaza ukwakheka kwamaqhubu wamathambo wamaminerali.
[cf. Chenyu Chu; UJia Deng; YiNdoda; I-Yili Qu (2017): I-Green Tea Extracts Epigallocatechin-3-gallate Yezindlela Ezihlukile Zokwelapha. I-BioMed Research International Vol. 2017]