I-Hielscher Ultrasound Technology

Amakhemikhali Ahlanganiswe eLipid Nanoparticles nge-Ultrasonics

Izithwali zezidakamizwa ezinobukhulu obukhulu be-Nano zisetshenziselwa kabanzi ukuletha amakhompiyutha asebenzayo emithini kumaseli ahlosiwe. Ukuze ubethele izinto ezisebenzayo kwisithwali somuthi esine-bioavailability ephezulu, kusetshenziswa ama-nanoparticles aqinile we-lipid. I-Ultrasonic nano-emulsization kanye ne-encapsulation iyindlela ethembekile yokukhiqiza inani elikhulu lezinto ezisezingeni eliphakeme ze-nano ezifana ne-lipid nanoparticles, i-nano-ehleliwe ye-lipid carriers kanye ne-liposomes.

Phakathi kwalesi sifo esiwubhubhane se-COVID-19 Hielscher Ultrasonics sibeka yonke imiyalo yomkhakha wezemithi. Sicela ubuze ukucutshungulwa kwe-oda kusengaphambili kanye nokuhanjiswa kwempahla okusheshayo!

I-Ultrasonic Nano-Emulsification ne-Encapsulation

I-Sonication iyakwazi ukuphazamisa izigaba zamafutha nezamanzi futhi ixube amaconsi amancane e-oyili emanzini. Njengoba inqubo ye-emulsification ye-ultrasonic ingalawulwa ngokuqondile, i-emulsization ye-ultrasonic kanye nenqubo elandelayo yokwakha i-nanoparticles eqinile-yolid iyakwazi ukukhiqiza i-sonoparticles sonication ekhiqizayo ingakhiqiza ubukhulu obukhulu be-Droplet ngokuqhathaniswa nezindlela ezijwayelekile ze-emulsion.

Njengoba i-nano-emulsization ye-ultrasonic n encapsulation ingalawulwa ngokuqondile, inqubo ye-sonication ivumela ukulawulwa okuqondile kwesayizi eqinile ye-lipid nanoparticle nokulayishwa kwayo.

I-Ultrasonic nano-emulsification iphumela emulsions ekhuphukayo ye-nano, liposomes kanye nanoparticles eqinile-lipid yokulethwa kwezidakamizwa.

I-Ultrasonic nano-emulsation isetshenziselwa ukukhiqiza ama-nano-emulsions, ama-liposomes nama-nanoparticles aqinile. I-Sonication ikhiqiza ukusatshalaliswa kwe-droplet emincane kanye nezinhlayiya ezihlelwe ngendlela ethile.

Ngenkathi i-lipid nanoparticles (i-SLNs) enkulu yama-lipid ingalayishwa ngenqwaba ephezulu yezinto ezisebenzayo, ama-SNL amancane akhombisa ukukhuphuka kakhulu kwe-kinetics yokufaka kanye nesikhathi esijikeleziwe sokusakazeka emzimbeni womuntu.

I-Hielscher Ultrasonics homogenizer ikunikeza ukulawula okugcwele kwamapharamitha enqubo anjenge-amplitude, izinga lokushisa, ingcindezi, isikhathi se-sonication nokufakwa kwamandla. Lokhu kukuvumela ukuthi ukhulise iresiphi eyenziwe ngezifiso yokwakheka kokuqina kwe-lipid nanoparticle. Ukulungiswa kwe-lipid nanoparticles kuhambelana nemithombo eminingi ye-lipid nama-emulsifiers.
I-Sonication iphumela ekuhanjisweni ngosayizi omncane wezinhlayiyana, ngakho-ke kuholela ekuzinzeni okuphezulu ngesikhathi sokulondolozwa.

I-encapsulation ye-Ultrasonic isetshenziselwa ukukhiqiza umthamo omkhulu we-liposomes ebangeni lezemithi ngomthwalo ophakeme wezinto ezisebenzayo.

I-UIP1000hdT ngomphumela wokugeleza kwengilazi ukukhiqizwa okuqinile kwe-lipid nanoparticle

Isicelo solwazi




Qaphela kwethu Inqubomgomo yobumfihlo.


Ukubethelwa kwe-Ultrasonic kwezinto zokwelapha kuma-nanoparticles e-lipid (SLNs)

Ukulungiswa kwe-Ultrasonic kweSolid-Lipid Nanoparticles
(Kumar et al. 2019)

Inzuzo ye-Ultrasonic Lipid Nanoparticle Ukulungiselela

  • Ukusebenza kahle okuphezulu
  • Ukulawulwa okuqondile kwesayizi yezinhlayiyana ze-lipid nomthwalo
  • Umthwalo ophezulu wezinto ezisebenzayo
  • Ukulawulwa okuqondile kwamapharamitha wenqubo
  • Inqubo Esheshayo
  • Ukulawulwa kwe-temp-non-Thermal okuqondile
  • ukusabalalisa okulinganayo
  • Reproducibility
  • Ukumiswa kwenqubo / GMP
  • Izinduna ezihlaselayo nezisebenzayo
  • I-CIP / SIP
Ukulungiswa kwe-Ultrasonic kwama-nanoparticles aqinile e-lipid kukhiqiza abathwali bezidakamizwa abahlelwe njengama-nano nge-bioavailability ephezulu, ukuqina okuphezulu kanye ne-cytotoxicity ephansi

Izinyathelo zokukhiqizwa kwe-ultrasonic zeSolid-Lipid Nanoparticles
(Kumar et al. 2019)

Ayini ama-Solid-Lipid Nanoparticles?

Ama-solid lipid nanoparticles (SLNs) ayindlela ejwayelekile yokuthwala izidakamizwa e-nano, engakwazi ukufaka amakhompiyutha we-bioactive noma wemithi. Bangakwazi ukuthuthukisa ukulethwa kwezidakamizwa kuma-lymphatics wamathumbu futhi bathuthukise ukungena ngaphakathi kwezicubu ngezinga eliphakeme lokulethwa kwama-Therapeutics. Ama-SNL angafaka izidakamizwa ze-lipophilic emgodleni wazo we-lipid, kanti ukugoqa okwenziwe ngenhla kwenza i-nanoparticle inganyibiliki emanzini futhi ibanikeze i-bioavailability ephezulu. Ama-SNL anesimo esiyindilinga futhi aqukethe umsuka oqinile we-lipid oqiniswe i-ejenti yokuqinisa (i-survivant, co-survivants).

Imithombo ejwayelekile ye-lipid ne-triglyceride yi-tricaprin, trilaurin, trimyristin, i-tripalmitin, i-tristearin, i-glyceryl monstearate, i-glyceryl behenate, i-glyceryl Palmitstearate, i-cetyl palmitate, i-acid ye-stearic, i-palmitic acid, i-decanoic acid, i-behenic acid, i-glycol estes.

Njengoba i-survivant kuma-lipid nanoparticles aqinile ama-lecithin (isib. I-soy lecithin, i-lecithin yeqanda, i-lecithin yamaqanda), i-phospholipids, i-phosphatidylcholine, i-sphingomyelins, i-bile salts (i-sodium taurocholate), ama-sterols (cholesterol), i-poloxamer 188, 182, 18olo, 182, 182, 182, 182, 182, 182, 182, 182, 182, 182, 182, 182, 182, 182, 182, 182. , i-polysorbate 20, 60, ne-80, i-sodium cholate, i-sodium glycocholate, i-taurocholic acid sodium usawoti, i-taurodeoxycholic acid sodium usawoti, butanol, butyric acid, dioctyl sodium sulfosuccinate, monooctylphosphoric acid sodium phakathi kwabanye isetshenzisiwe.

Njengoba ama-nanoparticles e-lipid aqinile aqukethe ama-lipids afanayo ngokomzimba atholakala emzimbeni womuntu, abhekwa njengabekezeleleke kahle. Zingahanjiswa ngomlomo, ngaphakathi nangaphakathi.

Ama-Ultrasonicators aphezulu enza umsebenzi we-Solid Lipid Nanoparticles

Ukulawulwa okuqondile kwamapharamitha wenqubo we-ultrasonic yi-software ekhaliphile ye-Hielscher UltrasonicsIzinhlelo ze-Hielscher Ultrasonics 'zisetshenziselwa kabanzi ekwenziweni kwemithi nokwengezekisa ukwakha ama-nanoparticles aphakeme aphezulu we-lipid nama-liposomes afakwe izinto zemithi, amavithamini, ama-antioxidants, ama-peptides nezinye izinto ezihlanganisiwe. Ukuhlangabezana nezidingo zamakhasimende ayo, abakwaHielscher bahlinzeka ngama-ultrasonicators avela ku-compact, nokho enamandla aphethwe ngesandla ama-lab homogeniser nama-bench-top ultrasonicators ezinhlelweni ze-ultrasonic zezimboni ngokugcwele zokukhiqizwa kwamanani aphezulu wezinqubo zokwenziwa kwemithi. Uhla olubanzi lwama-sonotrodes we-ultrasonic kanye nama-reactors ayatholakala ukuqinisekisa ukusethwa okuphezulu komkhiqizo wakho we-liposome. Ukuba namandla kwemishini yokusebenza kwe-Hielscher sika-Hielscher kuvumela ukusebenza kwe-24/7 emsebenzini osindayo nasezindaweni ezifunwayo.
Ukuze uvumele amakhasimende ethu ukuthi afeze i-Good yokukhiqiza imisebenzi (i-GMP) futhi asungule izinqubo ezihleliwe, wonke ama-ultrasonicators edijithali afakwe isoftware ehlakaniphile yokusetha ngokunembile ipharamitha ye-sonication, ukulawulwa kwenqubo okuqhubekayo nokuqoshwa okuzenzakalelayo kwawo wonke amapharamitha wenqubo abalulekile esakhiweni -Kukhadi le-SD. Ikhwalithi ephezulu yomkhiqizo incike ekulawulweni kwenqubo nakumazinga wokuphakama aphezulu ngokuqhubekayo. Ama-ultrasonicators we-Hielscher akusiza ukuthi uqaphele futhi wenze inqubo yakho ibe ng linye!
Ithebula elingezansi linikeza isibonakaliso somthamo wokucubungula we-ultrasonicators wethu:

Iqoqwana Ivolumu flow Rate Amadivayisi ezinconyiwe
1 kuya ku-500mL 10 kuya ku-200mL / min UP100H
10 kuya ku-2000mL 20 kuya ku-400mL / min UP200Ht, UP400St
0.1 kuya ku-20L 0.2 kuya ku-4L / min I-UIP2000hdT
10 kuya ku-100L 2 kuya ku-10L / iminithi I-UIP4000hdT
na 10 kuya ku-100L / iminithi UIP16000
na sikhulu yinhlanganisela UIP16000

Xhumana nathi! / Cela Us!

Cela ulwazi oluthe xaxa

Uyacelwa ukuthi usebenzise ifomu elingezansi ukucela ulwazi olungeziwe mayelana nezinhlelo zokusebenza ze-ultrasonic, izinhlelo zokusebenza nentengo. Sizokujabulela ukuxoxa ngenqubo yakho nawe futhi sikunikeze uhlelo lwe-ultrasonic oluhlangabezana nezidingo zakho!









Sicela uqaphele wethu Inqubomgomo yobumfihlo.


I-Hielscher Ultrasonics yenza ama-homogenizer aphezulu we-ultrasonic wokuhlakazeka, i-emulsation kanye nokukhishwa kweseli.

Amandla aphezulu we-homogenizer ephezulu I-Lab kuya umshayeli futhi Izimboni isikali.

Izincwadi / Izinkomba

  • Basarkar A., Devineni D., Palaniappan R., Singh J. (2007): Ukulungiselela, ukubekwa uphawu, ukusebenza kwe-cytotoxicity kanye nokudluliselwa kokusebenza kahle kwe-poly (D, L-lactide- co-glycolide) ne-poly (D, L-lactic acid) i-cationic nanoparticles yokulethwa okulawulwayo kwe-DNA ye-plasmid. Int J Pharm 343: 247-2525.
  • Zhao K., Li W., Huang T., Luo X., Chen G., Zhang Y., Guo Ch.,
    I-Dai Ch., Jin Z., Zhao Y., Cui H., Wang Y. (2013): Ukulungiselela kanye nokusebenza kwe-Newcastle Disease Virus DNA Vaccine Kuhlanganiswe ku-PLGA Nanoparticles. I-PLoS ONE 8 (12), 2013.


Amaqiniso Okufanele Ukwazi

I-Solid Lipid Nanoparticles njenge-Drug Carriers

Ama-nanoparticles aqinile we-lipid ngokuvamile anomumo oyindilinga obubanzi obuphakathi kwamamitha angama-10 kuye kwangama-1000. Ama-nanoparticles aqinile we-lipid ane-matrix eqinile ye-lipid lapho ama-molecule we-lipophilic angaxazululwa. Isisekelo se-lipid singaba nezakhi ezihlukile zamafutha, okusho ukuthi igama elithi "lipid" lisetshenziswe ngomqondo obanzi ohlanganisa ama-triglycerides (isib. Tristearin), diglycerides (isib. Glycerol bahenate), monoglycerides (isb. Glycerol monstearate), amafutha acid (isib. ), ama-steroid (isib. cholesterol), kanye ne-waxes (isib. cetyl Palmitate). Umgogodla we-lipid uqiniswa (ikakhulu inhlanganisela yama-ejenti we-emulsifying agents (i-survivant) akhethwe ngokuya ngendlela yokuphatha.

Imijovo ye-RNA

Imithi yokugomela ye-RNA isebenzisa ama-RNA (mRNA) okwenziwe ngemithi evikela amaprotheni aqhamuka ebusweni begciwane Lezo zingqa ze-mRNA zihlanganiswe kuma-nanoparticles e-lipid aqinile ukuze kuvuse impendulo yamasosha omzimba abantu kuleligciwane.