I-Hielscher Ultrasound Technology

Amakhemikhali Ahlanganiswe eLipid Nanoparticles nge-Ultrasonics

Izithwali zezidakamizwa ezinobukhulu obukhulu be-Nano zisetshenziselwa kabanzi ukuletha amakhompiyutha asebenzayo emithini kumaseli ahlosiwe. Ukuze ubethele izinto ezisebenzayo kwisithwali somuthi esine-bioavailability ephezulu, kusetshenziswa ama-nanoparticles aqinile we-lipid. I-Ultrasonic nano-emulsization kanye ne-encapsulation iyindlela ethembekile yokukhiqiza inani elikhulu lezinto ezisezingeni eliphakeme ze-nano ezifana ne-lipid nanoparticles, i-nano-ehleliwe ye-lipid carriers kanye ne-liposomes.

Phakathi kwalesi sifo esiwubhubhane se-COVID-19 Hielscher Ultrasonics sibeka yonke imiyalo yomkhakha wezemithi. Sicela ubuze ukucutshungulwa kwe-oda kusengaphambili kanye nokuhanjiswa kwempahla okusheshayo!

I-Ultrasonic Nano-Emulsification ne-Encapsulation

I-Sonication iyakwazi ukuphazamisa izigaba zamafutha nezamanzi futhi ixube amaconsi amancane e-oyili emanzini. Njengoba inqubo ye-emulsification ye-ultrasonic ingalawulwa ngokuqondile, i-emulsization ye-ultrasonic kanye nenqubo elandelayo yokwakha i-nanoparticles eqinile-yolid iyakwazi ukukhiqiza i-sonoparticles sonication ekhiqizayo ingakhiqiza ubukhulu obukhulu be-Droplet ngokuqhathaniswa nezindlela ezijwayelekile ze-emulsion.

Njengoba i-nano-emulsization ye-ultrasonic n encapsulation ingalawulwa ngokuqondile, inqubo ye-sonication ivumela ukulawulwa okuqondile kwesayizi eqinile ye-lipid nanoparticle nokulayishwa kwayo.

I-Ultrasonic nano-emulsification iphumela emulsions ekhuphukayo ye-nano, liposomes kanye nanoparticles eqinile-lipid yokulethwa kwezidakamizwa.

I-Ultrasonic nano-emulsation isetshenziselwa ukukhiqiza ama-nano-emulsions, ama-liposomes nama-nanoparticles aqinile. I-Sonication ikhiqiza ukusatshalaliswa kwe-droplet emincane kanye nezinhlayiya ezihlelwe ngendlela ethile.

Whilst larger solid lipid nanoparticles (SLNs) can be loaded with a higher concentration of active substances, small-sized SNLs show greatly increased kinetics of absorption and lengthened circulation time in the human organism.

I-Hielscher Ultrasonics homogenizer ikunikeza ukulawula okugcwele kwamapharamitha enqubo anjenge-amplitude, izinga lokushisa, ingcindezi, isikhathi se-sonication nokufakwa kwamandla. Lokhu kukuvumela ukuthi ukhulise iresiphi eyenziwe ngezifiso yokwakheka kokuqina kwe-lipid nanoparticle. Ukulungiswa kwe-lipid nanoparticles kuhambelana nemithombo eminingi ye-lipid nama-emulsifiers.
I-Sonication iphumela ekuhanjisweni ngosayizi omncane wezinhlayiyana, ngakho-ke kuholela ekuzinzeni okuphezulu ngesikhathi sokulondolozwa.

I-encapsulation ye-Ultrasonic isetshenziselwa ukukhiqiza umthamo omkhulu we-liposomes ebangeni lezemithi ngomthwalo ophakeme wezinto ezisebenzayo.

I-UIP1000hdT ngomphumela wokugeleza kwengilazi ukukhiqizwa okuqinile kwe-lipid nanoparticle

Isicelo solwazi




Qaphela kwethu Inqubomgomo yobumfihlo.


Ukubethelwa kwe-Ultrasonic kwezinto zokwelapha kuma-nanoparticles e-lipid (SLNs)

Ukulungiswa kwe-Ultrasonic kweSolid-Lipid Nanoparticles
(Kumar et al. 2019)

Inzuzo ye-Ultrasonic Lipid Nanoparticle Ukulungiselela

  • Ukusebenza kahle okuphezulu
  • Ukulawulwa okuqondile kwesayizi yezinhlayiyana ze-lipid nomthwalo
  • Umthwalo ophezulu wezinto ezisebenzayo
  • Ukulawulwa okuqondile kwamapharamitha wenqubo
  • Inqubo Esheshayo
  • Ukulawulwa kwe-temp-non-Thermal okuqondile
  • ukusabalalisa okulinganayo
  • Reproducibility
  • Ukumiswa kwenqubo / GMP
  • Izinduna ezihlaselayo nezisebenzayo
  • I-CIP / SIP
Ukulungiswa kwe-Ultrasonic kwama-nanoparticles aqinile e-lipid kukhiqiza abathwali bezidakamizwa abahlelwe njengama-nano nge-bioavailability ephezulu, ukuqina okuphezulu kanye ne-cytotoxicity ephansi

Izinyathelo zokukhiqizwa kwe-ultrasonic zeSolid-Lipid Nanoparticles
(Kumar et al. 2019)

Ayini ama-Solid-Lipid Nanoparticles?

Ama-solid lipid nanoparticles (SLNs) ayindlela ejwayelekile yokuthwala izidakamizwa e-nano, engakwazi ukufaka amakhompiyutha we-bioactive noma wemithi. Bangakwazi ukuthuthukisa ukulethwa kwezidakamizwa kuma-lymphatics wamathumbu futhi bathuthukise ukungena ngaphakathi kwezicubu ngezinga eliphakeme lokulethwa kwama-Therapeutics. Ama-SNL angafaka izidakamizwa ze-lipophilic emgodleni wazo we-lipid, kanti ukugoqa okwenziwe ngenhla kwenza i-nanoparticle inganyibiliki emanzini futhi ibanikeze i-bioavailability ephezulu. Ama-SNL anesimo esiyindilinga futhi aqukethe umsuka oqinile we-lipid oqiniswe i-ejenti yokuqinisa (i-survivant, co-survivants).

Imithombo ejwayelekile ye-lipid ne-triglyceride yi-tricaprin, trilaurin, trimyristin, i-tripalmitin, i-tristearin, i-glyceryl monstearate, i-glyceryl behenate, i-glyceryl Palmitstearate, i-cetyl palmitate, i-acid ye-stearic, i-palmitic acid, i-decanoic acid, i-behenic acid, i-glycol estes.

Njengoba i-survivant kuma-lipid nanoparticles aqinile ama-lecithin (isib. I-soy lecithin, i-lecithin yeqanda, i-lecithin yamaqanda), i-phospholipids, i-phosphatidylcholine, i-sphingomyelins, i-bile salts (i-sodium taurocholate), ama-sterols (cholesterol), i-poloxamer 188, 182, 18olo, 182, 182, 182, 182, 182, 182, 182, 182, 182, 182, 182, 182, 182, 182, 182, 182. , i-polysorbate 20, 60, ne-80, i-sodium cholate, i-sodium glycocholate, i-taurocholic acid sodium usawoti, i-taurodeoxycholic acid sodium usawoti, butanol, butyric acid, dioctyl sodium sulfosuccinate, monooctylphosphoric acid sodium phakathi kwabanye isetshenzisiwe.

Njengoba ama-nanoparticles e-lipid aqinile aqukethe ama-lipids afanayo ngokomzimba atholakala emzimbeni womuntu, abhekwa njengabekezeleleke kahle. Zingahanjiswa ngomlomo, ngaphakathi nangaphakathi.

Ama-Ultrasonicators aphezulu enza umsebenzi we-Solid Lipid Nanoparticles

Ukulawulwa okuqondile kwamapharamitha wenqubo we-ultrasonic yi-software ekhaliphile ye-Hielscher UltrasonicsIzinhlelo ze-Hielscher Ultrasonics 'zisetshenziselwa kabanzi ekwenziweni kwemithi nokwengezekisa ukwakha ama-nanoparticles aphakeme aphezulu we-lipid nama-liposomes afakwe izinto zemithi, amavithamini, ama-antioxidants, ama-peptides nezinye izinto ezihlanganisiwe. Ukuhlangabezana nezidingo zamakhasimende ayo, abakwaHielscher bahlinzeka ngama-ultrasonicators avela ku-compact, nokho enamandla aphethwe ngesandla ama-lab homogeniser nama-bench-top ultrasonicators ezinhlelweni ze-ultrasonic zezimboni ngokugcwele zokukhiqizwa kwamanani aphezulu wezinqubo zokwenziwa kwemithi. Uhla olubanzi lwama-sonotrodes we-ultrasonic kanye nama-reactors ayatholakala ukuqinisekisa ukusethwa okuphezulu komkhiqizo wakho we-liposome. Ukuba namandla kwemishini yokusebenza kwe-Hielscher sika-Hielscher kuvumela ukusebenza kwe-24/7 emsebenzini osindayo nasezindaweni ezifunwayo.
Ukuze uvumele amakhasimende ethu ukuthi afeze i-Good yokukhiqiza imisebenzi (i-GMP) futhi asungule izinqubo ezihleliwe, wonke ama-ultrasonicators edijithali afakwe isoftware ehlakaniphile yokusetha ngokunembile ipharamitha ye-sonication, ukulawulwa kwenqubo okuqhubekayo nokuqoshwa okuzenzakalelayo kwawo wonke amapharamitha wenqubo abalulekile esakhiweni -Kukhadi le-SD. Ikhwalithi ephezulu yomkhiqizo incike ekulawulweni kwenqubo nakumazinga wokuphakama aphezulu ngokuqhubekayo. Ama-ultrasonicators we-Hielscher akusiza ukuthi uqaphele futhi wenze inqubo yakho ibe ng linye!
Ithebula elingezansi linikeza isibonakaliso somthamo wokucubungula we-ultrasonicators wethu:

Iqoqwana Ivolumu flow Rate Amadivayisi ezinconyiwe
1 kuya ku-500mL 10 kuya ku-200mL / min UP100H
10 kuya ku-2000mL 20 kuya ku-400mL / min UP200Ht, UP400St
0.1 kuya ku-20L 0.2 kuya ku-4L / min I-UIP2000hdT
10 kuya ku-100L 2 kuya ku-10L / iminithi I-UIP4000hdT
na 10 kuya ku-100L / iminithi UIP16000
na sikhulu yinhlanganisela UIP16000

Xhumana nathi! / Cela Us!

Cela ulwazi oluthe xaxa

Uyacelwa ukuthi usebenzise ifomu elingezansi ukucela ulwazi olungeziwe mayelana nezinhlelo zokusebenza ze-ultrasonic, izinhlelo zokusebenza nentengo. Sizokujabulela ukuxoxa ngenqubo yakho nawe futhi sikunikeze uhlelo lwe-ultrasonic oluhlangabezana nezidingo zakho!









Sicela uqaphele wethu Inqubomgomo yobumfihlo.


I-Hielscher Ultrasonics yenza ama-homogenizer aphezulu we-ultrasonic wokuhlakazeka, i-emulsation kanye nokukhishwa kweseli.

Amandla aphezulu we-homogenizer ephezulu I-Lab kuya umshayeli futhi Izimboni isikali.

Izincwadi / Izinkomba

  • Basarkar A., Devineni D., Palaniappan R., Singh J. (2007): Ukulungiselela, ukubekwa uphawu, ukusebenza kwe-cytotoxicity kanye nokudluliselwa kokusebenza kahle kwe-poly (D, L-lactide- co-glycolide) ne-poly (D, L-lactic acid) i-cationic nanoparticles yokulethwa okulawulwayo kwe-DNA ye-plasmid. Int J Pharm 343: 247-2525.
  • Zhao K., Li W., Huang T., Luo X., Chen G., Zhang Y., Guo Ch.,
    I-Dai Ch., Jin Z., Zhao Y., Cui H., Wang Y. (2013): Ukulungiselela kanye nokusebenza kwe-Newcastle Disease Virus DNA Vaccine Kuhlanganiswe ku-PLGA Nanoparticles. I-PLoS ONE 8 (12), 2013.


Amaqiniso Okufanele Ukwazi

I-Solid Lipid Nanoparticles njenge-Drug Carriers

Solid lipid nanoparticles typically have a spherical shape with an average diameter between 10 and 1000 nanometers. Solid lipid nanoparticles possess a solid lipid core matrix in which lipophilic molecules can be solubilized. The lipid core can consist of different fat compounds, meaning that the term “lipid” is used in wider sense that includes triglycerides (e.g. tristearin), diglycerides (e.g. glycerol bahenate), monoglycerides (e.g. glycerol monostearate), fatty acids (e.g. stearic acid), steroids (e.g. cholesterol), and waxes (e.g. cetyl palmitate). The lipid core is stabilized by (mostly a combination of) emulsifying agents (surfactant) that are chosen depending on the way of administration.

Imijovo ye-RNA

RNA vaccines use synthetic messenger RNA (mRNA) strands that encode proteins from the virus surface Those mRNA strands are encapsulated in solid lipid nanoparticles in order to provoke a response of the human immune system to the virus.