Isihloko sokusetshenziswa: "I-Ultrasonic Lysis nokuphazamiseka kweseli"
I-Lysis yili gama elichaza ukuphazanyiswa kwezindonga zamaseli noma izibilini. Ukuphazamiseka kwe-Ultrasonic kuyithuluzi elithembekile lokuphula amangqamuzana ukuze kukhululwe ama-intracellular njenge-DNA, amaprotheni, ama-organelles, nama-phytochemicals. I-ultrasonic lysis (aka cell cell disruption), amagagasi aphezulu aphezulu / amavolumu ama-ultrasound asetshenziselwa ukuphazamisa izitho zangasese. Njengoba i-ultrasonication ikhuthaza ukudluliselwa kwamandla, impahla ye-intracellular ikhishwa kwi-solvent ejikelezile. Umswakama oqukethe okuqukethwe kwamaseli lensisi ubizwa ngokuthi i-lysate.
I-cell lysis iyisinyathelo sokuqala ekuqhekekeni kwamaseli, ukuhlukaniswa kwe-organelle kanye nokufakelwa kwamaprotheni nokuhlanzwa.
Funda kabanzi mayelana nama-ultrasonic cell disruptors ne-ultrasonic lysis!
Amakhasi angu-12 ngalesi sihloko ayakhonjiswa:
I-Cell Lysis yamaseli we-BL21 yi-Ultrasonication
Amaseli we-BL21 awuhlobo lwe-E.coli olusetshenziswa kakhulu kumalabhorethri ocwaningo, i-biotechnology kanye nokukhiqizwa kwezimboni ngenxa yamandla abo okuveza amaprotheni asebenza kahle kakhulu. Ukuphazamiseka kwamaseli e-Ultrasonic, ukukhishwa kwe-lysis kanye namaprotheni kuyindlela ejwayelekile…
https://www.hielscher.com/cell-lysis-of-bl21-cells-by-ultrasonication.htm
Ultrasonic Sample Prep for ELISA Assays
Izilingo ezifana ne-ELISA zisetshenziselwa kabanzi ukuxilongwa kwe-in vitro, ukutholwa kwamaprotheni ahlobene nezifo nokulawulwa kwekhwalithi (isb. Ukulungiswa kwesampula ye-Ultrasonic kuyindlela esheshayo, ethembekile futhi ekhiqizwayo yokwenza iseli nokuhlukanisa amaprotheni angaphakathi, i-DNA, i-RNA…
https://www.hielscher.com/ultrasonic-sample-prep-for-elisa-assays.htm
Iphrothokholi ye-SARS-CoV-2 Coronavirus Inactivation ne-Sonication
I-Hielscher VialTweeter iyunithi eyingqayizivele yokulungiselela amasampula amaningi we-ultrasonic, esetshenziselwa ukungasebenzi i-coronavirus SARS-COV-2. I-VialTweeter ivumela ukulungiselela izigaxa zesampula ezifika ku-10 ngasikhathi sinye futhi ngaleyo ndlela iyunithi ekahle yokucubungula amasampula amaningi.…
https://www.hielscher.com/protocol-for-sars-cov-2-coronavirus-inactivation-with-sonication.htm
Ukulungiselela Isampula nge-Ultrasonic VialTweeter
Ukulungiselelwa kwesampula ngaphambi kokuhlaziywa kungadinga izinqubo ezahlukahlukene zangaphambi kokuhlaziywa ezinjenge-homogenization yezicubu, i-lysis, ukukhishwa kwamaprotheni, i-DNA, i-RNA, i-organelles nezinye izinto ezingaphakathi kwamangqamuzana, ukuqedwa nokususwa. IVialTweeter iyidivayisi eyingqayizivele ye-ultrasonic elungiselela amashubhu amaningi wesampula…
https://www.hielscher.com/sample-preparation-with-the-ultrasonic-vialtweeter.htm
Production Biosynthetic of Human Milk Oligosaccharides
I-biosynthesis ye-oligosaccharides yobisi lomuntu (i-HMOs) ngokusebenzisa ukuvutshelwa noma ukusabela kwe-enzymatic inqubo eyinkimbinkimbi, edla isikhathi futhi evame ukuthambisa. I-Ultrasonication ikhulisa ukudluliswa okuyisisekelo phakathi kwamafektri amancane namaseli kuvuselela ukukhula kwamaseli kanye nokuqina kokudla. Ngaleyo ndlela, sonication iyaqina…
https://www.hielscher.com/human-milk-oligosaccharides.htm
Ama-Ultrasonic Dismembrators Asebenzayo Kakhulu
Ama-dismembrators we-Ultrasonic asetshenziswa kakhulu endaweni yokucwaninga, ebhentshini kanye nasemkhakheni ukulungiselela ukumiswa okungahambi kahle kwama-colloidal anjenge-emulsions nokusabalalisa ngama-droplet noma osayizi bezinhlayiyana ku-submicron- ne-nano-range. Ngaphezu kwalokho, ama-dismembrators e-ultrasonic asetshenziselwa ukuphazamisa iseli,…
https://www.hielscher.com/most-efficient-ultrasonic-dismembrators.htm
Ama-Ultrasonic Disintegrators
Ukuqhekeka kwamangqamuzana kuwumsebenzi ojwayelekile ku-biotechnology nasezintweni eziningi ezibandakanya ukucubungula amaseli nezinto ezibonakalayo ze-intracellular. Ama-distrigrators amaseli e-Ultrasonic ayasebenza futhi athembekile, uma kuziwa ekuphuleni udonga lweseli noma ulwelwesi lwamaseli wezitshalo, ama-mammalian…
https://www.hielscher.com/ultrasonic-disintegrators.htm
ICoronavirus (COVID-19, SARS-CoV-2) ne-Ultrasonics
I-Ultrasonication iyithuluzi elinamandla elisetshenziswa ku-biology, chemistry chemistry kanye ne-biochemistry nasekukhiqizweni kwemithi yemithi. I-Bio-science isebenzisa ama-homogenizer e-ultrasonic ukwenza ama-lyse cell futhi akhiphe amaprotheni nezinye izinto eziwusizo, imboni ye-pharma isebenzisa ama-ultrasonics…
https://www.hielscher.com/coronavirus-covid-19-sars-cov-2-and-ultrasonics.htm
I-Ultrasonics ekucwaningweni kwegciwane
I-Ultrasonic lysis kanye nokukhishwa kuyindlela ethembekile futhi yesikhathi eside esungulwe yokuphazamiseka kwamaseli nokukhululwa okulandelayo kwamagciwane, amaprotheni egciwane, iDNA, ne-RNA. I-Ultrasonics ekucwaningweni kweCoronavirus Ukukhishwa kwamagciwane kusuka kwizicubu zezitho…
https://www.hielscher.com/ultrasonics-virus-research.htm
I-Ultrasonic Cold-Water Extraction ye-Mogrosides kusuka ku-Monk Fruit
Izithelo zeMonki (S. grosvenorii) zikhipha futhi ikakhulukazi i-mogroside yayo eyi-V isetshenziswa njenge-sweetener engokwemvelo engeyona i-caloric. I-Mogrosides. Ukukhishwa kwe-Ultrasonic kuyindlela elula, esheshayo, yokuzala futhi enokwethenjelwa esetshenziswe ngempumelelo ukukhiqiza i-non-caloric sweetener evela emoneleni…
https://www.hielscher.com/ultrasonic-cold-water-extraction-of-mogrosides-from-monk-fruit.htm
Ukuhlukaniselana Kwezintathu Zama-Ultrasonically Enhanced
Okwahlukaniswa ngezigaba ezintathu (TPP) kuyindlela yokukhipha, ukuhlukanisa nokuhlanza izakhi, isib. Ama-lipids, ama-enzyme, ama-polysaccharides namanye ama-biomolecule, ezintweni ezibonakalayo. I-ultrasonically isize ukwahlukaniswa kwezigaba ezintathu kudlula okujwayelekile kwe-TPP ngesivuno esiphakeme, ubumsulwa obuthuthukisiwe nejubane elihlukile.…
https://www.hielscher.com/ultrasonically-enhanced-three-phase-partitioning.htm
Ultrasonic Psilocybin Extraction kusuka Amakhomikhali
I-Psilocybin yinto esebenzayo ekhishwe kumakhowe we-hallucinogenic. Isizinda se-Ultrasonic sivame ukusetshenziselwa ukukhipha i-psilocybin kahle kusuka kumakhowe. Isizinda se-Ultrasonic sinikeza isivuno esikhulu se-psilocybin ngesikhathi sokukhipha esisheshayo. Ukuhlukaniswa kwe-Ultrasonic kwe-Psilocybin Isizinda se-Ultrasonic…
https://www.hielscher.com/ultrasonic-psilocybin-extraction-from-mushrooms.htm