I-Hielscher Ultrasound Technology

Indlela Yokusebenzisa i-Hielscher's Ultrasonic Tissue Homogenizers

Ngaphambi kokuhlanzwa noma ukuhlukaniswa kwe-macromolecule yama-intracellular njengama-protini, ama-organelles, ama-enzyme noma ama-compounds asebenzayo, indlela ephumelelayo ye-tissue lysis ne-cell disintegration iyadingeka. I-ultrasonication iyindlela ephumelelayo yokulungiselela iseli ekhishwa izinto ezingenayo i-intracellular kusuka ekamelweni langaphakathi leselili ngokushesha zibe yisisombululo se-buffer. I-mechanical shear ibutho le-ultrasonic izicubu ze-homogenization ingashintshwa ngokuqondile ematheksthini athile, ukulungiselela ukulungisa ukucupha (isib. I-DNA / RNA) nge-sonication emnene noma ukuphazanyiswa kwamaseli (isib. Nannochloropsis, imvubelo) nge-cavitation enkulu ye-ultrasonic.

Thola ngezansi ukukhethwa kwezicubu, amangqamuzana kanye nezinye izinto eziphilayo ezihambisana nezinqubo zokuxhumana nge-sonication nezincomo ezihlobene, ukuthi ungalungisa kanjani isampula ngempumelelo usebenzisa i-homogenizer ye-ultrasonic.

I-ultrasonication iyindlela ephumelelayo kakhulu yokulungiselela isampula kubandakanya izicelo zeseli lysis, ukuphazanyiswa, ukuhlakazeka & ukukhipha.

Ultrasonic izicubu ze-homogenizer UP100H

Indlela Yokulungisa Ama-Lysates akho, ama-Homogenates kanye ne-Extracts kusuka ku-Biological Materials

Ngo-alfabhethi:

Acetobacter suboxydans

Isicelo se-Ultrasonic:
Ukuphazanyiswa kwe-cell ku-5-15 sec.
Incomo yedivayisi:
UP200St

Actinomyces

Isicelo se-Ultrasonic:
Ukuphazanyiswa kanye ne-protein extraction ku-3-5 min.
Incomo yedivayisi:
UP50H

Umsebenzi we-ALP no-LDH

Isicelo se-Ultrasonic:
Ukunqunywa kwemisebenzi ye-ALP ne-LDH kanye nokuqukethwe kwamaprotheni
Amasampuli wamaseli afriziwe aphethwe ngamaminithi angu-20 eqhweni, alandelwe i-cell lysis ne-PBS equkethe i-1% ye-Triton X-100 ngamaminithi angu-50 eqhweni. Ngesikhathi se-cell lysis isampuli ngayinye yayinikwe nge-1 min ku-80 W ngeprosesa ye-ultrasonic UP100H (Hielscher Ultrasonics).
Incomo yedivayisi:
UP100H
Isikhombo / Iphepha Lokucwaninga:
UBernhardt, A. et al. (2008): I-collagen encane ye-mineralized, i-extracellular bone matrix-ihlukanisa ukuhlukaniswa kwe-osteogenic yamangqamuzana e-bone marrow stromal.

I-Amorphous tricalcium phosphate (ATCP)

Isicelo se-Ultrasonic:
I-ATCP i-nano-particles, eyisimo esiyimpumelelo esisebenzayo sokubunjwa kwe-hydroxyapatite, yahlakazeka ku-chloroform equkethe u-5% (w / w) Tween20 okubhekiswe ku-PLGA usebenzisa i-Hi400cher idivayisi ye-ultrasonic ultrasonic ku-320W ye-5 min. besebenzisa izikhathi ezinamaphesenti (50%) ukuvumela ukuphumula kwezinhlayiya.
Incomo yedivayisi:
UP400S
Isikhombo / Iphepha Lokucwaninga:
UMohn, uDerk; Ege, Duygu; Feldman, Kirifl; Schneider, Oliver D .; Imfeld, uTomas; I-Boccaccini, i-Aldo R. (2014): I-Spherical calcium phosphate i-nanoparticle fillers ivumela ukusetshenziswa kwama-polymer ama-bone fixation nge-bioactivity ephezulu. I-Library yamahhala 01 Meyi 2010. 21 Januwari 2014.

Anthocyanins

Isicelo se-Ultrasonic:
I-Anthocyanins: i-di-glucosides, i-mono-glucoside, i-monoglucosides ene-acylated nama-di-glucosides acylated we-peonidin, i-malvidin, i-cyanidin, i-petunidin ne-delphinidin: Ukukhipha kusuka esikhumbeni samagilebhisi ku-40 sec. pH 5.0; isilinganiso sezinto ezibonakalayo / izinto zokwenyuka kwe-1: 6.
Incomo yedivayisi:
UP100H

Anthraquinones

Isicelo se-Ultrasonic:
Ukukhishwa kwe-Anthraquinones kusukela ezimpandeni zikaMorinda citrifolia
Incomo yedivayisi:
UP400S

Imbewu ye-apricot

Isicelo se-Ultrasonic:
Ultrasonic pre-treatment ngaphambi kokukhipha amafutha embewu yeJatropha curcas L. ukuthuthukisa isizinda.
Incomo yedivayisi:
UP400S

Artemisia selengensis Turcz

Isicelo se-Ultrasonic:
Ukukhishwa kwesampula se-Rutin (isampuli ye-1.0g yama-militha ku-30 mL methanol) ku-25 ° C ku-5-10 iminithi.
Incomo yedivayisi:
UP50H

I-Aspergillus flavus

Isicelo se-Ultrasonic:
Inactivation ye-Ultrasonic ye-Aspergillus flavus eSabouraud medium medium
Incomo yedivayisi:
UP200S

B. sphaericus / Bacillus sphaericus

Isicelo se-Ultrasonic:
Ukuphazamiseka kumaminithi angu-1-3.
Incomo yedivayisi:
UP200S

Ama-bacillus anthracis sterne ama-34F2 nezinhlamvu

Isicelo se-Ultrasonic:
Ukususwa kwe-ultrasonic kwe-Bacillus anthracis sterne 34F2, i-Bacillus cereus ATCC 21281, kanye nezinhlamvu ze-Bacillus thuringiensis ATCC 33680. Ukusetshenziswa kwe-150 ppm ye-sodium hypochlorite kanye ne-ultrasound kwaholela ekusebenziseni kwe-spore.
Incomo yedivayisi:
UP200S ku-100% amplitude
Isikhombo / Iphepha Lokucwaninga:
Pamarthi, SR et al .: Ukuphumelela kwe-Ultrasound ku-Desoiling Bacillus spp spores Okufakiwe kwi-Complex Matrices Yokudla Okuxhunywe Kuzozonke Ezokuxhumana.

I-Bacillus cereus ATCC 21281 izinhlamvu

Isicelo se-Ultrasonic:
Ukususwa kwe-ultrasonic kwe-Bacillus anthracis sterne 34F2, i-Bacillus cereus ATCC 21281, kanye nezinhlamvu ze-Bacillus thuringiensis ATCC 33680. Ukusetshenziswa kwe-150 ppm ye-sodium hypochlorite kanye ne-ultrasound kwaholela ekusebenziseni kwe-spore.
Incomo yedivayisi:
UP200S ku-100% amplitude
Isikhombo / Iphepha Lokucwaninga:
Pamarthi, SR et al .: Ukuphumelela kwe-Ultrasound ku-Desoiling Bacillus spp spores Okufakiwe kwi-Complex Matrices Yokudla Okuxhunywe Kuzozonke Ezokuxhumana.

I-Bacillus subtilis

Isicelo se-Ultrasonic:
Amandla aphakeme, i-frequency ultrasound ephansi eminyanisweni encane yokumiswa kwebhaktheriya imiphumela ekunciphiseni okuqhubekayo ezinombeni zamangqamuzana ebhaktheriya, okusho ukuthi isilinganiso sokubulala siningi. Eminyanisweni emikhulu, i-sonication iholela ekukhuleni kokuqala ezinombeni zezinombolo ezikhomba ukuwohloka kwamabhaktheriya kodwa lokhu kuphakama kokuqala kwehla njengoba ukuphela kwehla kuqhubeka futhi isilinganiso sokubulala sibaluleke kakhulu.
Incomo yedivayisi:
UP200St
Isikhombo / Iphepha Lokucwaninga:
UJoyce, E .; Phull, SS; I-Lorimer, i-JP; Mason, TJ (2003): Ukuthuthukiswa nokuhlolwa kwe-ultrasound ekwelapheni ukumiswa kwamagciwane. Ukutadisha imvamisa, amandla kanye nesikhathi sokunikwa ingane kwizinhlobo ze-Bacillus ezikhuliswe. I-Ultrason. Sonochem. 10/2003. k. 315-318.

I-Barley's Alpha-amylase

Isicelo se-Ultrasonic:
Ukugqugquzela noma ukuvimbela umsebenzi we-Barley's Alpha-amylase: Isampula (imbewu engu-10 g yebhali) yahlakazeka ku-80 ml yamanzi kampompi ngesikhathi sokuneneka kwe-ultrasonic kwe-20, 60 ne-100% amplitude, i-cyle ka-50% kanye nokuqhushulwa okwengeziwe . I-sonotrode ibhapathizwa cishe ngo-9 mm kwisisombululo. Isixazululo sasiqhutshwa ekushiseni okungapheli kwama-30C ° ngezinombolo ezingu-5, 10 no-15.
Incomo yedivayisi:
UP200S, amplitudes: 20, 60 no-100%; i-cyle ye-50%; I-Sonotrode S3, 30C °.
Isikhombo / Iphepha Lokucwaninga:
Yaldagard et al. (2008): Imiphumela ye-Ultrasonic Power emsebenzini we-Barley's Alpha-amylase kusuka ekuthuleni okulandelwayo Ukuphathwa kwembewu

Barnacle nauplii

Isicelo se-Ultrasonic:
Ukuphazanyiswa kwamagciwane / ukubulawa kwe-mesozooplankton: ngokufaka ingane ngaphansi kwezimo ezine zamanani okugeleza (200, 400, 520 no-800 lh-1) kanye nama-amplitudes amane (25, 50, 75 no-100%) isilinganiso sokubulala esiphakathi kuka-61 no-97% kufeziwe.
Incomo yedivayisi:
I-UIP2000hd
Isikhombo / Iphepha Lokucwaninga:
Viitaslo et al. (2005): i-Ozone, i-Ultraviolet Light, i-Ultrasound ne-Hydrogen Peroxide Njengoba i-Ballast Water Treatments – Ukuhlola nge-Mesozooplankton E-Low Saline- Amanzi E-Brackish.

Izinkinga ze-Bifidobacteria: B. breve ATCC 15700, B. animalis subsp. I-Lactis (BB-12), B. longum (BB-46)

Isicelo se-Ultrasonic:
Ukubhujiswa kwamangqamuzana e-bacteria nokukhululwa kwe-ß-galactosidase emithonjeni ye-Bifidobacteria: B. breve ATCC 15700, B. animalis subsp. I-Lactis (BB-12), B. longum (BB-46): Ubisi olungamamitha angu-100 obunamakhemikhali obuhlanganiswe ne-bacteria culture kwasetshenziswa nge-ultrasound. I-cavitation ibangela ukubhujiswa kwamangqamuzana amabhaktheriya ngesikhathi esisodwa, ukukhululwa kwe-ß-galactosidase.
Incomo yedivayisi:
I-UIP1000hd: amplitude: 10%, amandla: 80W, amplitude: 100%, amandla: 200W; I-Sonotrode BS2d34; 15-30 iminithi.
Isikhombo / Iphepha Lokucwaninga:
Hung et al. (2009): Ukuvuthwa kwe-yogurt okusizwa nge-ultrasound nge-Probiotics.

BL21 (DE3) ama-pAtHNL amaseli (ama-Arabidopsis thaliana amaseli)

Isicelo se-Ultrasonic:
Ukuphazanyiswa kwamagciwane: 15 g BL21- (DE3) _pAtHNL amangqamuzana amiswa okwesikhashana ku-50mM potassium phosphate buffer (pH 7.5) ku-0 degC.
Incomo yedivayisi:
UP200S + i-sonotrode S14D: ku-70W / cm2 (4 x 5 min), ihlile ebhodini lokugeza
Isikhombo / Iphepha Lokucwaninga:
Okrob, D. et al (2009): I-Hydroxynitrile Lyase evela ku-Arabidopsis thaliana: Ukukhonjelwa Kwemigomo Yokwenziwa Kwe-Enantiopure Cyanohydrin Synthesis yi-Catalyst Ehlanzekile Nokungasebenzi. U-Adv. I-Synth. IsiCatal. 2011, 353, 2399 - 2408.

Amaseli egazi (obomvu nomhlophe)

Isicelo se-Ultrasonic:
Ukuphazamiseka kumasekhondi angu-3-10.
Incomo yedivayisi:
UP100H

I-Boldine evela eBoldo ishiya (U-Peumus boldus Molina)

Isicelo se-Ultrasonic:
I-composite ebalulekile esebenzayo ku-boldo yi-boldine ((S) -2,9-dihydroxy-1,10-dimethoxiaporphine), ngaphandle kwe-catechin ((2S, 3R) -2- (3,4-dihydroxy-phenyl) -3 , I-4-dihydro-1 (2H) -benzopyran-3,5,7-triol) izingxenye eziyinhloko zengxenyana ye-alkaloid ne-flavonoid emaqabunga e-boldo. I-Boldine i-agent enamandla e-antioxidant eyenza umonakalo ongenawo ama-peroxidative mahhala futhi usebenza njenge-scavenger eyi-hydroxyl esebenzayo.
Inqubo Yokususa: Ngenqubo evamile yokufakelwa, amasampula amaqabunga e-boldo akhishwa ngamanzi angu-1L emanzini angcolile ngomsindo womoya esebenzisa i-ultrasonicator I-UIP1000hd ku-batch ne-flow-through mode. Isikhathi samakamelo okukhipha phakathi kwamaminithi angu-10 no-40., Nge-Ultrasonic ngamandla we-10 kuya ku-23 W / cm2, kanye nobubanzi bokushisa obuphakathi kuka-10 kuya ku-70 ° C. Imiphumela emihle kakhulu iphumelele ngaphansi kwezimo ezilandelayo: ukuqina kwe-ultrasonic ka-23 W / cm2 iminyaka engu-40. ekushiseni kuka-36 ° C
Imiphumela: Imiphumela yokuhlaziya ibonisa ukuthi ukukhuliswa kwamandla amakhulu kukhulisa ukukhishwa kwe-analyte yempahla yemifino yaseBoldo ngamazinga angcono kakhulu uma kuqhathaniswa nendlela evamile: isivuno esifanayo sakhishwa ngo-sonication ngamaminithi angu-30. ngenkathi kuvamile isikhathi sokukhipha kwaba ngu-2h.
I-Chemat (2013) kanye nabasebenzi abambisene nabo baye babonisa ukuthi isizinda esisizwa nge-ultrasonically sithuthukisa ukusebenza kahle kwesitshalo sesitshalo ngenkathi kuncishiswe isikhathi sokukhipha ngesikhathi sokukhushulwa kweziqhumane (isamba esifanayo solvent and plant material). Ukuhlaziywa kwembula ukuthi izimo ezilungiselelwe kwakuyizi: amandla we-sonication 23 W / cm2 nge I-UIP1000hd iminyaka engu-40. kanye nokushisa kwama-36 ° C. Imiphakamiso ehlelwe yesikhumba se-ultrasound inikeza isizinda esingcono uma siqhathaniswa ne-maceration ejwayelekile ngokwemigomo yesikhathi senqubo (30 min esikhundleni semaminithi angu-120), isivuno esiphezulu, ukusebenza kahle kwamandla, ukuhlanzeka okuthuthukile, ukuphepha okuphakeme kanye nemfanelo yomkhiqizo ongcono.
Incomo yedivayisi:
I-UIP1000hd nge-sonotrode BS2d34 nokugeleza kweseli
Isikhombo / Iphepha Lokucwaninga:
I-Petigny, L .; I-Périno-Issartier, iS .; Wajsman, J .; Chemat, F. (2013): Isisetshenziswa Sokubambisana Ne-Continuous Ultrasound Esenziwe Nge-Boldo Leaves (Peumus boldus Mol.). I-International Journal ye-Molecular Science 14, 2013. 5750-5764.

I-bovine serum albin (BSA)

Isicelo se-Ultrasonic:
I-microencapsulation in poly (lactic-co-glycolic acid) engama-40 sec.
Incomo yedivayisi:
dmini
Isikhombo / Iphepha Lokucwaninga:
Freitas et al. (2005): I-flow-through emulsification e-ultrasonic ihlanganiswe ne-micromixing static ye-aseptic yokukhiqizwa kwe-microspheres nge-solvent isizinda.

I-Brain stem + i-adrenal gland

Isicelo se-Ultrasonic:
Ukuhlakazeka nokuhlaziywa kwe-nucleotid; Usayizi wesampula: amasampuli ama-10 mg ku-10 ml wamanzi.
Incomo yedivayisi:
UP50H

Candida albicans

Isicelo se-Ultrasonic:
Ukuphazanyiswa kwe-15mL ngo-9min.
Incomo yedivayisi:
UP100H

Ama-carbohydrate, i-polysaccharides nezinye izinhlanganisela zokusebenza

Isicelo se-Ultrasonic:
Ukwenziwa kwe-carbohydrate, i-polysaccharides nezinye izinhlanganisela zokusebenza
Incomo yedivayisi:
UP200St

I-Carnosic acid kusuka e-rosemary

Isicelo se-Ultrasonic:
Ukukhishwa kwe-carnosic acid, isakhi esisebenzayo, kusuka ku-rosemary.
Incomo yedivayisi:
UP400S

I-Capsaicinoids

Isicelo se-Ultrasonic:
Ukwenziwa kwe-capsaicinoids (i-capsaicin, i-nordihydrocapsaicin) kusuka e-chilipelepele: Ama-Capsaicinoids avela I-Capsicum frutescens i-pepper yatholakala nge-ultrasonic isizinda ngaphansi kwezimo ezilandelayo: i-solvent: 95% (v / v) ethanol, i-solvent / mass ratio ye-10 ml / g, i-40 min. sonication isikhathi extraction, 45 ° C lokushisa lokushisa. Ukuvunwa okweqile: 85% we-capsaicinoids
Incomo yedivayisi:
UP400S

I-Carotenoids, i-Beta-Carotenoids

Isicelo se-Ultrasonic:
Ukukhipha kusuka izaqathe.
Incomo yedivayisi:
UP50H

i-cDNA

Isicelo se-Ultrasonic:
I-Poly-A RNA yahlanzwa nge-kit Dynabeads mRNA yokuhlanza (i-Invitrogen) elandela imiyalelo yomkhiqizi futhi yaphathwa ngamaminithi angu-30 ku-37 ° C nge-TURBO DNase (Ambion; 0.2 amayunithi / 1 μg ye-RNA). I-first-and-second-strand synthesis ilandele umkhondo womkhiqizi. Cishe i-500ng ye-cDNA ephindwe kabili ihlukaniswe ukuxhunyaniswa no-Hielscher UTR200. I-DNA yayibanjwe nge-2% ephezulu ye-agarose gel kanye nama-230-270 ama-bp fragments aqoshiwe.
Incomo yedivayisi:
UTR200 noma TD_CupHorn
Isikhombo / Iphepha Lokucwaninga:
U-Delft, J. van; Gaj, St .; U-Lienhard, M .; I-Albrecht, MW; I-Kirpiy, A .; Ama-Brauers, K .; Claessen, S .; I-Lizarraga, D .; I-Lehrach, H .; U-Herwig, uR .; I-Kleinjans, J. (2012): I-RNA-Seq inikeza ulwazi olusha emaphepheni we-Transcriptome Ekhishwe yi-Carcinogen Benzo [i-pyrene]. Sciences Toxicological 130/2, 2012. 427-439.

I-Caryophanon latum

Isicelo se-Ultrasonic:
Ukukhishwa kwe-glucosamine, i-muramic acid, i-alanine, i-glutamic acid ne-lysine ukusuka ku-caryophanon datum.
Incomo yedivayisi:
UP100H

I-cellulose

Isicelo se-Ultrasonic:
Ukwenziwa kwe-homogenization / ukulungiselela iselulosi e-isotopic e-homogeneous.
Incomo yedivayisi:
UP200S; ebhodini lokugeza
Isikhombo / Iphepha Lokucwaninga:
Laumer et al. (2009): Inqubo entsha ye-homogenization ye-cellulose ukusebenzisa ama-microamakha ukuze kuhlaziywe isotopu okuzinzile.

I-cellulose nanocrystals (CNC) elungiselelwe kusuka kuma-CNCs eucalyptus cellulose

Isicelo se-Ultrasonic:
I-Cellulose nanocrystals (CNC) elungiselelwe kusuka ku-CNCs eucalyptus cellulose yashintshwa yi-reaction methyl adipoyl chloride, CNCm, noma inhlanganisela ye-acetic ne-sulfuric acid, i-CNCa. Ngakho-ke, ama-CNC, ama-CNC, ama-CNCa ashintshiwe, aphinde aphinde aphinde avuselelwe ku-solvents ehlanzekile (EA, THF noma i-DMF) ku-0.1 wt%, i-bymagnetic evuselela ubusuku (24 ± 1) C, ilandelwa ngamaminithi angu-20 ebhodini lokufaka i-sonication usebenzisa i-UP100H Hielscher Ultrasonics (EJalimane), ifakwe ne-sonotrode eyi-130 W / cm2, ku-24 ± 1 degC. Ngemuva kwalokho, i-CAB yanezelwa ekuhlakazeni kwe-CNC, ukuze ukuhlushwa kokugcina kwe-polymer kube yi-0.9 wt%.
Incomo yedivayisi:
UP100H; ku-deg deg 24 ngamaminithi angu-20.
Isikhombo / Iphepha Lokucwaninga:
U-Blachechen, uLS et al (2013): Ukusebenza kwe-colloidal ukusimama kwama-cellulose ama-crystaltic and dispersibility in cellulose i-acetate butyrate matrix. Cellulose 2013.

I-cellulose kusuka esikhwameni soshukela

Isicelo se-Ultrasonic:
Ukwenziwa kwe-cellulose kusuka esikhwameni se-sugar
Incomo yedivayisi:
UP200St

Ukuhlolwa kwe-ChIP

Isicelo se-Ultrasonic:
I-Ultrasonication isetshenziselwa i-cell lysis ukukhulula i-chromatin. I-sonication emnene (pulsed) isetshenziselwa ukuhlukanisa i-chromatin. Ngaphezu kwalokho, i-ultrasound iyasheshisa izinga le-antibody elibophayo ukuze libheke amaprotheni futhi linciphise isikhathi sokuzivikela.
Incomo yedivayisi:
UP100H
Isikhombo / Iphepha Lokucwaninga:
Basselet, P. et al. (2008): Ukucubungula kwesampula se-DNA kuhlaziywa ngokuhlaziywa okusekelwe ngokulinganayo kwe-Escherichia coli (EHEC) enempilo.
U-Lauri, A. (2005): Ukuhlaziywa kwamangqamuzana okuthuthukiswa kwezintambo nge-X-ChIP kanye nobuchwepheshe obunobunjabulo ababili. I-Dissertation University yaseCologne 2005.

I-Chromatin

Isicelo se-Ultrasonic:
Ukukhishwa kwe-chromatin
Incomo yedivayisi:
UP400S; ku-30% amplitude kanye nomjikelezo we-0.5; phezu kweqhwa.
Isikhombo / Iphepha Lokucwaninga:
Oh et al. (2003): I-Acetyl-CoA i-Carboxylase Gene ilawulwa yi-Protein-1 ebophezelayo e-Eter Regulatory Element.

Isizinda se-Chromatin

Isicelo se-Ultrasonic:
I-lysate yamaseli e-MEL DS19 ahanjiswe ngama-rounds angu-20 ka-20 s ngalinye ngamaphesenti angama-70 okuphezulu okukhipha usebenzisa i-Hi200cher 200W i-proxy ultrasonic UP200H
Incomo yedivayisi:
UP200H; I-70% yokukhishwa okuphezulu; Imodi ye-pulse: imijikelezo engu-10 ye-20 sec. ngamunye.
Isikhombo / Iphepha Lokucwaninga:
Kang, H. Ch. et al (2010): I-PIAS1 ilawula ukusekwa kwe-CP2c nokwakheka okuyinkimbinkimbi okugqugquzelayo ekwakheni i-crystal-specific specific-globin expression. I-Nucleic Acids Res. 38/16, 2010. iphe. 5456-5471.

I-Chromatography

Isicelo se-Ultrasonic:
I-Sonication ye-adsorbant ku-solvent isusa ama-agglomerate phakathi kwemizuzwana embalwa futhi ilungisa ikholomu, ifakwe kalula. Ukubheka ukulungiswa kwe-adsorbent (isb. I-gel silika) ngaphambi kwe-chromatography yekholomu.
Incomo yedivayisi:
UP400S

Ama-Cladocerans

Isicelo se-Ultrasonic:
Ukuphazamiseka kweselula kwe-mesozooplankton
Incomo yedivayisi:
UP2000hd nge-flow cell
Isikhombo / Iphepha Lokucwaninga:
Viitaslo et al. (2005): i-Ozone, i-Ultraviolet Light, i-Ultrasound ne-Hydrogen Peroxide Njengoba i-Ballast Water Treatments – Ukuhlola nge-Mesozooplankton E-Low Saline- Amanzi E-Brackish.

Abantu abadala be-Copepod nama-copepodites

Isicelo se-Ultrasonic:
Ukuphazanyiswa kweselula kwe-mesozooplankton: ngokufaka ingane ngaphansi kwezimo ezine zokuhamba kwamanzi (200, 400, 520 no-800 lh-1) kanye nama-amplitudes amane (25, 50, 75 no-100%) isilinganiso sokubulala esiphakathi kwama-87 no-99% siphumelele .
Incomo yedivayisi:
I-UIP2000hd nge-flow cell
Isikhombo / Iphepha Lokucwaninga:
Viitaslo et al. (2005): i-Ozone, i-Ultraviolet Light, i-Ultrasound ne-Hydrogen Peroxide Njengoba i-Ballast Water Treatments – Ukuhlola nge-Mesozooplankton E-Low Saline- Amanzi E-Brackish.

I-Copepod nauplii

Isicelo se-Ultrasonic:
Ukuphazanyiswa kweselula kwe-mesozooplankton: ngokufaka ingane ngaphansi kwezimo ezine zokuhamba kwamanzi (200, 400, 520 no-800 lh-1) kanye nama-amplitudes amane (25, 50, 75 no-100%) isilinganiso sokubulala esiphakathi kwama-87 no-99% siphumelele .
Incomo yedivayisi:
I-UIP2000hd nge-flow cell
Isikhombo / Iphepha Lokucwaninga:
Viitaslo et al. (2005): i-Ozone, i-Ultraviolet Light, i-Ultrasound ne-Hydrogen Peroxide Njengoba i-Ballast Water Treatments – Ukuhlola nge-Mesozooplankton E-Low Saline- Amanzi E-Brackish.

COS7-amaseli

Isicelo se-Ultrasonic:
Lysis ku-400 μl buffer
Incomo yedivayisi:
UP200S; Imijikelezo engu-7
Isikhombo / Iphepha Lokucwaninga:
I-Zaim (2005): Hlaziya i-Nesprin-2 Defizienten Mäusen.

I-Cryptosporidium iphazamisekile

Isicelo se-Ultrasonic:
Inactivation ye-ultrasonic ye-Cryptosporidium parvaum (i-protozoa) emanzini.
Incomo yedivayisi:
UP400S
Isikhombo / Iphepha Lokucwaninga:
Tsukamoto, I .; I-Yim, B .; I-Stavarache, CE; I-Furuta, M .; Hashiba, K .; I-Maeda, Y. (2004): Ukungasebenzi kwe-Saccharomyces cerevisiae nge-irradiation ye-ultrasonic. I-Ultrason. Sonochem. 11/2004. k. 61-65.

Cubosomes

Isicelo se-Ultrasonic:
Cubosomes – kungaba okungenalutho noma okufakwe amakhemikhali e-fluorophore – zazilungiswa ngokuhlakaza inani elifanele le-monoolein kusixazululo sePluronic F108 besebenzisa i-ultrasonicator UP100H. Ukuze bathole ama-cubosomes e-fluorescent, i-fluorophore yahlakazeka e-monoolein encibilikile nge-sonification encane ngaphambi kokuhlakazeka ku-Pluronic F108. Inqubo efanayo yalandelwa lapho ama-cubosomes e-fluorescent nawo alayishwa nge-quercetin.
Isibonelo: usayizi wesampula: 4 mL – cishe. 96.4% wamanzi, 3.3 wt% we-monoolein, i-0.3 wt% ye-Pluronic F108. Iphesenti ye-fluorophore yayingu-2.5 × 10-3 no-2.8 × 10-3%. Inani le-quercetin elongeziwe: 6.4 × 10-6%.
I-Sonication: UP100H, i-amplitude engu-90%, umjikelezo we-pulse 0.9, we-10 min.
Incomo yedivayisi:
UP100H
Isikhombo / Iphepha Lokucwaninga:
I-Murgia, iS .; Bonacchi, S .; I-Falchi, AM; Lampis, S .; Lippolis, V .; Ukuhamba, i-V .; I-Monduzzi, M .; I-Prodi, L .; Schmidt, J .; I-Talmon, Y .; I-Caltagirone, C. (2013): I-Cubosomes ye-Fluorescent ephethe izidakamizwa: I-nanoparticles ehlukahlukene yokusetshenziswa kwezicelo ze-Theranostic. Langmuir 29, 2013. 6673-6679.

Dekkera bruxellensis

Isicelo se-Ultrasonic:
Inactivation ye-ultrasonic ye-Dekkera bruxellensis emanzini
Incomo yedivayisi:
I-UIP1500hd
Isikhombo / Iphepha Lokucwaninga:
I-Borthwick, i-KAJ; Coakley, WT; UMcDonnell, MB; I-Nowotny, H .; Benes, E .; Grfschl, .M (2005): Ukuthuthukiswa kwe-sonicator ye-compact inoveli ye-cell disruption. J. Microbio. Ameth. 60/2005. iphe. 207-216. / Lörincz, A. (2004): Ukuphazanyiswa kwamaselula e-ultrasonic kwemvubelo emisekelweni esekelwe ngamanzi. Ama-Biosys. Eng. 89 / 2004. kk. 297-308. / Tsukamoto, I .; I-Yim, B .; I-Stavarache, CE; I-Furuta, M .; Hashiba, K .; I-Maeda, Y. (2004): Ukungasebenzi kwe-Saccharomyces cerevisiae nge-irradiation ye-ultrasonic. I-Ultrason. Sonochem. 11/2004. k. 61-65.

I-Dekkera / i-Brettanomyces bruxellensis

Isicelo se-Ultrasonic:
Inactivation in 90-120 sec.
Incomo yedivayisi:
I-UIP1500hd
Isikhombo / Iphepha Lokucwaninga:
bheka ngenhla

DNA

Isicelo se-Ultrasonic:
Ukuhlukaniswa kwe-DNA: 2 iminithi. i-sonication ye-100μL ne-UP100H noma i-4 min. sonication of 100μL nge-UTR200.
Incomo yedivayisi:
UP100H, UTR200 noma I-VialTweeter
Isikhombo / Iphepha Lokucwaninga:
Larguinho M. et al. (2010): Ukuthuthukiswa kweqhinga le-ultrasonic elisuselwe ekuqhekekeni kwe-DNA.

Amaseli e-Drosophila melanogaster S2

Isicelo se-Ultrasonic:
Ukukhipha amaprotheni weselula kusuka kumaseli e-Drosophila melanogaster S2: Amaseli ama-S2 amafrijini (1.56108) ahlolwe ku-1 mL i-homogenization buffer (m 100mM Tris-HCl pH 7.5, 1% SDS) futhi ishiya kwiqhwa ngamaminithi angu-10. I-cell lysis yaqedwa yi-ultrasonication eqhweni (i-6615 bursts, i-0.5 s pulse; i-75% ngamandla) nge-Hielscher UP200S i-processor ultrasonic.
Incomo yedivayisi:
UP200S (200W), i-75% ye-intensitypulse mode: 6615 i-bursts, i-0.5 s ye-puls.
Isikhombo / Iphepha Lokucwaninga:
Schwientek, T. et al. (2007): Indlela ye-lectin ejwayelekile ye-mucin-O-glycoproteome yama-Drosophila melanogaster S2 cells. I-Proteomics 7, 2007. iphe. 3264-3277.

Iziqephu ze-E. coli

Isicelo se-Ultrasonic:
Ukuphazanyiswa kwamagciwane okutholakala kwe-E. coli: I-pellets efriziwe ehambisana nevolumu yesampula yeVculture = 4 / OD mL yabuye yavuselelwa ku-580 μL 10mM i-potassium phosphate buffer pH 7, 1 mM EDTA. I-20 μL ye-lysozyme (ukuxilongwa kwe-1 g L-1) yanezelwa futhi ukumiswa kwamaseli kwakhishwa kwiqhwa cishe okungama-30 min. Emva kwalokho amaseli aphazanyiswa yi-ultrasonication eqhubekayo ne-ultrasonicator (UP 200S Ultraschallprozessor, uDkt. Hielscher GmbH, iTeltow) eqhweni, nge-amplitude ka-50% okwama-20. Izingxenyana zezingqamuzana ezingenayo futhi ezingenasici zahlukaniswa yi-centrifugation ngo-13000 rpm ngamaminithi angu-20. Amaprotheni angenakulinywa ahlanzwa kabili nge-10mM potassium phosphate buffer pH 7, 1mM EDTA futhi agcinwe ku -20 ° C.
Incomo yedivayisi:
UP200S nge-50% amplitude
Isikhombo / Iphepha Lokucwaninga:
I-HA (2005): Ukuthuthukisa ukukhiqizwa kwamaprotheni okuvuselelayo okusebenzayo, ukuhlola umthelela wamaprotheni amancane okushisa e-Escherichia coli, i-IbpA ne-IbpB, ekusebenziseni kabusha imizimba yokufaka.

I-Echinococcus granulosus Antigen

Isicelo se-Ultrasonic:
I-cell lysis kanye nokuhlukaniswa: Isampula esakhiwe ngokweziqu ze-protein e-Egranulosus evuthiwe, elungiselelwe ukukhishwa kwe-nitrogen ne-42◦C, ihanjiswe ku-110V, 170W ye-3×15 amasekhondi eqhweni. Ngemuva kwalokho, isampula yayikhululwe nge-centrifugated ngamaminithi angu-15 ku-10000g. Ama-protein concentaration ayalinganiswa ngendlela yaseBradford futhi agcinwe ku--20◦C.
Incomo yedivayisi:
UP200S; 170W, ukupholisa eqhweni; 3 x 15 sec.
Isikhombo / Iphepha Lokucwaninga:
I-Tabar et al. (2010): I-Serodiagnosis ye-Sheep Hydatidosis ne-Hydatid Fluid, i-Protoscolex ne-Whole Body ye-Echinococcus granulosus Antigen.

Escherchia coli Gr-

Isicelo se-Ultrasonic:
Ukungasebenzi kwe-Ultrasonic ka-Escherchia coli Gr- ebisi namanzi.
Incomo yedivayisi:
UP100H
Isikhombo / Iphepha Lokucwaninga:
UZenker, M .; I-Heinz, i-V .; I-Knorr, D. (2003): Ukusetshenziswa kwe-ultrasound okusetshenziselwa ukucubungula okushisayo kokulondolozwa nokugcinwa kwekhwalithi yokudla okuketshezi. J Food Prot 66/2003. amakhasi 1642-1649.

Escherchia coli Gr- in saline

Isicelo se-Ultrasonic:
Inactivation ye-ultrasonic ye-Escherchia coli Gr- ku-saline.
Incomo yedivayisi:
UP100H
Isikhombo / Iphepha Lokucwaninga:
I-Duckhouse, H .; Mason, TJ; Phull, SS; I-Lorimer, i-JP (2004): Umphumela we-sonication kwi-microbial disinfection usebenzisa i-hypochlorite. I-Ultrason. Sonochem. 11 / 2004. kk. 173-176.

Escherchia coli Gr- emanzini

Isicelo se-Ultrasonic:
Inactivation ye-ultrasonic ye-Escherchia coli Gr- emanzini.
Incomo yedivayisi:
UP100H
Isikhombo / Iphepha Lokucwaninga:
I-Furuta, M .; I-Yamaguchi, M .; Tsukamoto, T .; I-Yim, B .; I-Stavarache, CE; Hasiba, K .; Maeda, Y. (2004): Ukungasebenzi kwe-Escherichia coli nge-ultrasonic irradiation. I-Ultrason. Sonochem. 11/2004. k. 57-60.

I-glaucoma, ikhanda le-nerve optical

Isicelo se-Ultrasonic:
Ukuhlukaniswa kwe-RNA yamakhanda we-optical (kusuka kwamehlo aphikisayo): Inhloko yamagundane e-optical (ONH) yayineqhwa eqhweni elomile futhi igcinwe ku-80 ° C ngaphambi kokukhipha. I-RNA yayingasondelene nokubeka izingqungquthela zama-frozen ezinqamule ngaphakathi kwesikhungo sokukhipha i-MS nge-MS 0.5 (UP50H) emva kwalokho, emva kwemiyalelo ehlinzekwa yi-kit Arcturus, okufaka ukwelashwa kwe-DNase ukususa noma iyiphi i-DNA. I-RNA ehlanzekile yayinqunywe.
Incomo yedivayisi:
UP50H nge probe MS0.5
Isikhombo / Iphepha Lokucwaninga:
Johnson et al. (2007): Izinguquko Zomhlaba Emkhakheni We-Optic Nerve Head Gene ngemuva kokuvezwa kokucindezelwa kwe-Intraocular Extension in a Rat Glaucoma Model.

I-Glycosaminoglycan chondroitin sulfate (CS)

Isicelo se-Ultrasonic:
Ukunquma CS nge-Dimethylmethylene Blue Assay
Ukuvuselelwa kwamaseli: Ama-pellets CS e-microcentrifuge tubes aphinde avuselelwe ku-0.5 ml yesisombululo sika-0.1mg / ml se-papain kwisixazululo sosawoti esisezingeni likaHank (HBSS) esebenzisa amapulisi avela ku-horn ultrasound (UP 100H, Hielscher Ultrasonics GmbH, eJalimane) emjikelezweni wokuqala 1 I-100% amplitude ye-3 sec. Ngemva kwalokho, ukugaya kwenzeka ngo-60 ° C ngehora lesithupha.
Ukuhlolwa kwe-immunosorbent (ELISA) ye-Enzyme, amaseli amiswa okwesikhashana emanzini ahlanjululwe ngamanzi angama-106 / ml, futhi agcinwe efrijini ku -70 ° C ngobusuku obubili ukuze akwazi ukusiza i-cell lyysis. Amaseli aphinde ahlanjululwe yi-ultrasonication ngamasekhondi angu-40. usebenzisa i-Hielscher ultrasonic tissue homogenizer UP100H.
Incomo yedivayisi:
UP100H
Isikhombo / Iphepha Lokucwaninga:
Vandrovcova et al. (2011): Uthonya lwe-Collagen ne-Chondroitin Sulfate (CS) Izembatho ze-Poly- (Lactide-co-Glycolide) (i-PLGA) ku-MG 63 I-Osteoblast-Njengezinhlamvu. I-Physiol. Res. 60; 2011. 797-813.

Amaseli we-HaCaT

Isicelo se-Ultrasonic:
I-Lysis yamaseli e-HaCaT we-Chromatin Immunoprecipitation (i-ChIP): amaseli we-HaCaT ahlelwe nge-1% e-formdehyde imizuzu engu-10 ku-37uC. Amaseli ahleliwe ahlotshiswe ku-RIPA futhi ahlinzekwa ngeqhwa nge-100W idivayisi ye-ultrasonic ku-amplitude = 1 nomjikelezo womsebenzi = 100% emaminithini angu-12 (12 x 1 min).
Incomo yedivayisi:
UP100H; amaminithi angu-12. phezu kweqhwa,
Isikhombo / Iphepha Lokucwaninga:
Zhang et al. (2007): I-Basonuclin Ilawula i-Risetom RNA Genetic ku-HaCaT Amaseli.

I-Heparin: I-depolymerization ye-heparin

Isicelo se-Ultrasonic:
Indlela ye-ultrasonic yenza ukhiqize isisindo somzimba we-heparin (LMWH) esincane. Ngakho-ke, i-heparin ilawulwa yi-hydrogen peroxide-catalyzed depolymerization. Ukusabela kuyashesha kakhulu futhi kuthatha isikhathi esingaphansi kwehora elilodwa, ngenkathi inqubo ye-physicochemical depolymerization incike ezimweni zokuphendula kahle, akudingi ama-reagents anesibindi noma enobuthi, futhi inikeza umkhiqizo mahhala wempahla yamakhemikhali. Ngakho-ke, inqubo ye-ultrasonic ihambelana kahle nokukhiqizwa okukhulu kwe-LMWH, kodwa futhi ama-analogs akhiqizwa kusuka kuma-polysaccharides engokwemvelo.
Inqubo ye-ultrasonic:
Ukuze i-deplorymerization ye-physicochemical ye-heparin engahlanjululwa yi-ultrasonic-eyasiza kakhulu i-depolymerization enkulu, i-heparin yachithwa emanzini kuya ekujuleni kokugcina kuka-25 mg / mL (5 ml). Ingxube yokusabela yayisetshenziswa nge-ultrasoundator UP50H. I-ultrasonicator yayinezibhamu (i-tip micro MS3) enebubanzi obuyi-3mm, eyanikeza ukuphakama kwe-180μm. Iprosesa yakhiqiza ukuxilisa kwe-longitudinal mechanical nge-frequency ka-30 kHz okwakhiqizwa ukuxolisa kagesi. Ingxube yokusabela yayigcinwa ngo-60 ° C ku-reactor ye-Radleys® namagagasi e-ultrasonic asetshenziswa njenge-0.5sec impulse ukuvimbela ukushisa kwenhlanganisela. I-aliquot ye-zero (250μl) isusiwe isisombululo ngaphambi kokusungulwa kokusabela ngokuhlanganisa okwesikhashana i-hydrogen peroxide kanye nokusetshenziswa kwamagagasi e-ultrasonic. I-hydrogen peroxide yanezelwa ukuze uthole isilinganiso sokugcina se-hydrogen peroxide / heparin (w / w) isilinganiso esingu-0.15 (3.75 mg / mL).
Incomo yedivayisi:
UP50H nge-sonotrode MS3
Isikhombo / Iphepha Lokucwaninga:
I-Achour, i-Oussama; UBridiau, uNicolas; U-Allahhbani, u-Azza; I-Joubioux, i-Florian; UBordenave Juchereau, uStephanie; USannier, uFredéric; UPiot, uJean-Marie; UFruitier Arnaudin, u-Ingrid; UMaugard, Thierry (2013): Ukulungiswa kwe-Ultrasonic okusikali esincane se-heparin (LMWH) esine-anticoagulant. I-Carbohydrate Polymers 97; 2013. 684-689.

Hops

Isicelo se-Ultrasonic:
Ukwenziwa kwamakhemikhali asebenzayo / ama-extracts emifino emanzini nase-ethanol.
Incomo yedivayisi:
UP400S

I-Lactobacillus (i-lysis / i-DNA ukuhlukaniswa kwezinkinga ezihlukahlukene ze-Lactobacillus)

Isicelo se-Ultrasonic:
Izinkinga ze-Lactobacillus: L. pontis, L. sanfranciscensis, L. farciminis, L. panis, L. oris, L. vaginalis noL. reuteri, L. sp.
Inqubo: Ukuze kuhlukaniswe i-DNA yamakholomu angasha, i-ultrasonic lysis protocol yasungulwa. I-colony eyodwa (ububanzi obuphakathi kuka-2 kuya ku-3-mm) inqanyuliwe ku-100μl i-lysis buffer (i-EDM 20mM, i-TM 10 ye-Tris [pH 7.9], i-1% ye-Triton X-100, i-guamine-HCl engu-250mM, i-NaCl engu-250mM. Amaseli ahlolwe ngamaminithi angu-1 we-ultrasonication nge-probe-type ultrasonicator UP50H. Ngemuva kokungezwa kwe-ethanol ebandayo engu-150μl (-20 ° C), ingxube yayiyi-centrifuged phezu kwekholomu ye-QIAamp yekitiki futhi i-elit 60% ye-tampue (10mM Tris [pH 7,5]).
Ukuze ube nethuluzi lokukhomba okusheshayo nokwethenjelwa kwamasiko ahlanzekile angabodwa, i-PCR isilingo yayihlanganiswe nenqubo yokuhlukanisa i-DNA esheshayo. Izinqubo zokuqeda i-enzymatic lysis kanye nokutholakala okuguquguqukayo kwama-bacteria kuya kwi-lysozyme zahlukunyezwa ukwelashwa kwe-ultrasonic yamaseli, ngokuhlanzwa okulandelayo nokugxila ngokubopha i-DNA kumatrix we-silica. Izinto ezigciniwe zekholomu eyodwa zitholakala ukuthi zanele i-PCR.
Incomo yedivayisi:
UP50H
Isikhombo / Iphepha Lokucwaninga:
UMüller, i-MRA (2000): Ukufaniswa kwe-Microbial Ecosystem ye-Fertilization yeCereal esebenzisa i-Molecular Biological Methods. I-Dissertation University of Cologne, 2000.

I-Lactobacillus acidophilus Gr +

Isicelo se-Ultrasonic:
Inactivation ye-Ultrasonic ye-Lactobacillus acidophilus Gr + ebisi namanzi.
Incomo yedivayisi:
UIP500hd
Isikhombo / Iphepha Lokucwaninga:
UZenker, M .; I-Heinz, i-V .; I-Knorr, D. (2003): Ukusetshenziswa kwe-ultrasound okusetshenziselwa ukucubungula okushisayo kokulondolozwa nokugcinwa kwekhwalithi yokudla okuketshezi. J Food Prot 66/2003. amakhasi 1642-1649.

I-Legionella i-pneumophila Gr + kuphakathi okuhlanjululwe

Isicelo se-Ultrasonic:
Inactivation ye-ultrasonic yeLegionella pneumophila Gr + kuphakathi okuhlanjululwe.
Incomo yedivayisi:
UIP500hd
Isikhombo / Iphepha Lokucwaninga:
Ubaba, MF; Ogino, C .; Matsumura, S .; Nakamura, S .; UShimizu N. (2006): Ukuqothulwa kweLegionella pneumophila nge ukwelapha ultrasonic nge TiO2. Amanzi Res 40/2006. pp.1137-1142.

Leuconostoc mesenteroides

Isicelo se-Ultrasonic:
Umsebenzi we-Leukocyte lysozme ku-myelocytic leukemia: ukumiswa kweseli kwahanjiswe iminyaka engu-15. futhi amasampuli ahlolwe umsebenzi we-lysozyme. Ukuhlushwa kwe-lysozyme yamaseli we-leukocytes ug./10 kunqunywe.
Incomo yedivayisi:
UP50H

Umsebenzi we-leukocytic isozym ku-myelocytic leukemia

Isicelo se-Ultrasonic:
Ukulungiswa kwesampula: ukumiswa kweli cell kwakuphathwa nge-ultrasonically futhi kusampuli kulingwa ngomsebenzi we-lysozyme. Umxube we-lysozyme we-leukocytes ug / 106 wawunqunywe.
Incomo yedivayisi:
UP200St

I-Malachite Green

Isicelo se-Ultrasonic:
Ukuchithwa kwe-Malaop Green (i-bactericide eqinile): Ukuchithwa kwe-photocatalytic kuphela okusheshayo kunokuphulukiswa kwe-sonolytic, ukusebenza kahle kungathuthukiswa ngokuhlanganisa lezi zinqubo ezimbili. I-malachite eluhlaza, i-bactericide eqinile, i-ecotoxic kakhulu kuma-bacteria emanzini kodwa iguqulwa ibe yizinto ezingokwemvelo ezingaphansi noma ezingekho ezinobuthi.
Incomo yedivayisi:
UP400S

Mangiferin acylation

Isicelo se-Ultrasonic:
I-Mangiferin (1,3,6,7-Tetrahydroxy-2- [3,4,5-trihydroxy-6- (hydroxymethyl) oxan-2-yl] xanthen-9-eyodwa; ifomula: C19H18O11) i-polyphenol ye-C-glycosylxanthone isakhiwo esingatholakala ezinhlotsheni eziningi zezitshalo. I-Mangiferin ibonisa imisebenzi ehlukahlukene yokwelapha. I-regioselective acylation ye-mangiferin ingenziwa kahle kakhulu nge-lipase ngaphansi kwe-ultrasonication. Uma kuqhathaniswa nezindlela ezijwayelekile, i-catalysis eyayisizwa nge-ultrasonically ihamba phambili ngezinzuzo zesikhathi esifushane sokuphendula isikhathi kanye nezitshalo eziphakeme. Izimo ezinhle ze-ultrasonic mangiferin acylation zitholakala njengezilandelayo:
lipase: PCL, acyl donor: i-vinyl acetate; yokusabela solvent: DMSO, ukusabela kokushisa: 45 degC, amandla ultrasonic: 200W; i-substrate ratio: i-acyl donor / mangiferin 6/1, ukulayisha i-enzyme: 6 mg / ml
I-regioselective acylation isivuno yayingama-84%.
Incomo yedivayisi:
UP200St noma UP200Ht
Isikhombo / Iphepha Lokucwaninga:
cp .: Wang, Z .; Wang, R .; I-Tian, ​​J .; I-Zhao, B; I-Wei, i-XF; I-Su, YL; Li, CY; I-Cao, SG; Wang, L. (2010): Umphumela we-ultrasound ku-lipase-catalyzed regioselective acylation of mangiferin ezingxenyeni ezingenayo i-aqueous solvents. J. Asian Nat Prod. Res. 12/1, 2010. 56-63.

Ukukhishwa kwamakhemikhali

Isicelo se-Ultrasonic:
I-protocol ye-Extraction ye-extraction kusuka ku-gypsum, i-rheolite, i-basalt, i-edfell ash ne-glass obsidian:
Isampula ye-1g ye-regolith noma idwala elichotshoziwe lingaphansi kwesisetshenziswa samanzi ngaphansi kwe-ultrasonic irradiation ukuze kukhishwe futhi kuhlaziye umgomo wamakhemikhali. Ngakho-ke, i-3ml MeOH P80 yanezelwa ku-1g isampula ye-analogue ku-tube yokuhlola ingilazi futhi ihanjiswe ngomzuzu wama-20 nge-ultrasonic i-probe-type device UP50H ihlelwe ngamaphesenti angu-40%. Le nhlanganisela yavunyelwa ukuma imizuzu engu-10, futhi ama-supernatant amafu abekwe ngaphezu kwesampula esilungisiwe esasilungisiwe yayingena emathinini ayi-1.5 ml we-centrifuge. Ukuze unciphise ukulahlekelwa kwezingxenye ezikhishwe ngokufaka izikhangiso ezindaweni eziphezulu zamatayipi we-polyphobic centrifuge, izilimo eziyizigaxa zavinjelwa ngo-0.5% (w / v) BSA ku-100mM i-HEPES pH 7.4. I-supernatants yacaciswa yi-centrifugation ku-17000 G imizuzu engu-10 futhi igcinwe ku-2 - 8 ° C emigodini yeglasi kuze kuhlolwe i-immunoassay.
Incomo yedivayisi:
UP50H
Isikhombo / Iphepha Lokucwaninga:
I-Rix, C. (2012): Ukuthola Ukuphila ku-Mars ne-Chip Marker Chip: Antibody Assays Yokuthola Amakhemikhali Engokwemvelo Ekuqotheni Kwamafutha Amasampuli A-Martian. I-Dissertation Cranfield University 2012.

Amapulangwe we-fox ye-mouse

Isicelo se-Ultrasonic:
Ama-pellets ahlanjululwa futhi ahanjiswa ngamaminithi angu-5 nge-0.5 mL ye-LB2 futhi agxile ku-12,000 g enye imaminithi angu-20, kanti izingxenye ezimbili ze-supernatant zaqoqwa. Ekugcineni, ama-pellets ahlakazwa nge-0.5 mL ye-buffer equkethe ama-tris base-40mM, ama-5 M urea, ama-2 M thiourea, ama-4% ama-CHAPS, i-100 mM DTT, i-0.5% (v / v) i-biolyte 3-10 (LB3), futhi i-sonicated futhi igxile ku-12,000 g ngamaminithi angu-20.
Incomo yedivayisi:
UP200S; 5min.
Isikhombo / Iphepha Lokucwaninga:
I-Gazzana et al. (2009): Isibuyekezo ku-mouse yeproteome ye-mouse.

Ukumiswa kwesibindi se-Mouse

Isicelo se-Ultrasonic:
I-homogenization yesampula ukukhiqiza i-lysate yeseli.
Incomo yedivayisi:
UP200S; for 3 x 20 sec.
Isikhombo / Iphepha Lokucwaninga:
I-Gazzana et al. (2009): Isibuyekezo ku-mouse yeproteome ye-mouse.

I-Penicillium digitatum

Isicelo se-Ultrasonic:
Inactivation ye-ultrasonic ye-Penicillium digitatum (i-pathogen yesitshalo) eSabouraud medium medium.
Incomo yedivayisi:
UP200St
Isikhombo / Iphepha Lokucwaninga:
López-Malo, A .; I-Palou, E .; UJiménez-Fernández, uM .; I-Alzamora, SM; UGuerrero, S. (2005): Ukusebenza okungekho emthethweni okuhlanganisa ukungena kwe-thermosonication kanye nama-antimicrobial. J. Food Eng. 67 / 2005. kk. 87-93.

I-Phycocyanin evela ku-Spirulina platensis (i-Arthrospira platensis)

Isicelo se-Ultrasonic:
Ukwenziwa kwe-phycocyanin kusuka ku-Spirulina platensis (amaseli e-Arthrospira platensis).
Incomo yedivayisi:
UP400S

Amaseli ezitshalo kanye nezicubu zezitshalo

Isicelo se-Ultrasonic:
I-30% yamaseli esitshalo ahlanganisiwe (W / V) namanzi adidliziwe aphazanyiswa yi-sonication ngamaminithi angu-1-15; ukutshala izicubu ezincibilikisiwe: 1 g izicubu ezomisiwe eziphuze utshwala zihlukaniswe ngesikhathi sokubeletha cishe ngamaminithi amahlanu.
Incomo yedivayisi:
UP100H

Platelets

Isicelo se-Ultrasonic:
Ukulungiswa kwe-Platelet lysate: Ukuphazanyiswa ngo-1-5 min.
Incomo yedivayisi:
UP200Ht

Pleurotus tuberregium

Isicelo se-Ultrasonic:
Ukukhishwa kwe-polysaccharides kusuka ku-fungus edliwayo Pleurotus tuberregium
Incomo yedivayisi:
UP400S

I-poly (i-lactic-co-glycolic acid) (i-PLGA)

Isicelo se-Ultrasonic:
Ukulungiselela i-poly-lactic-co-glycolic acid (i-lactic-co-glycolic acid) (PLGA) nge-sonication ku-40 sec.
Incomo yedivayisi:
dmini; for 40sec.
Isikhombo / Iphepha Lokucwaninga:
Freitas et al. (2005): I-flow-through emulsification e-ultrasonic ihlanganiswe ne-micromixing static ye-aseptic yokukhiqizwa kwe-microspheres nge-solvent isizinda.

Polyphenols kusuka apula

Isicelo se-Ultrasonic:
Isizinda se-Ultrasonic se-polyphenols kusuka ku-apula. I-Solvent: i-aqueous. Ukuvunwa okwandisiwe ngo-6%; ukuqina kwe-sonication: 20-75Ws / ml; inqubo yesikhashana: ukufuthelwa ku-80 degC.
Incomo yedivayisi:
I-UIP2000hd
Isikhombo / Iphepha Lokucwaninga:
I-Vilkhu, K .; UManase, R .; Mawson, R .; U-Ashokkumar, M. (2011): Ukubuyiswa kwe-ultrasonic nokuguqulwa kokudla izithako. Ku: Feng / Barbosa-Cánovas / Weiss (2011): Ubuchwepheshe be-Ultrasound for Food and Bioprocessing. I-New York: Springer, 2011. iphe. 345-368.

Polyphenols kusuka itiye omnyama

Isicelo se-Ultrasonic:
Isizinda se-ultrasonic se-polyphenols esitokisini esimnyama. I-Solvent: i-aqueous. Ukuvunwa okwandisiwe ngo-6-18%; ukuqina kwe-sonication: 8-10Ws / ml; ukucindezela okukhulu, inqubo yesikhashana: ukufuthelwa ku-90 degC.
Incomo yedivayisi:
I-UIP2000hd
Isikhombo / Iphepha Lokucwaninga:
I-Vilkhu, K .; UManase, R .; Mawson, R .; U-Ashokkumar, M. (2011): Ukubuyiswa kwe-ultrasonic nokuguqulwa kokudla izithako. Ku: Feng / Barbosa-Cánovas / Weiss (2011): Ubuchwepheshe be-Ultrasound for Food and Bioprocessing. I-New York: Springer, 2011. iphe. 345-368.

Polyphenols kusuka marc omvini obomvu

Isicelo se-Ultrasonic:
Isizinda se-ultrasonic se-polyphenols kusuka emarcini omvini obomvu. I-Solvent: i-aqueous. Ukuvunwa okwandisiwe ngo-11-35%; ukuqina kwe-sonication: 20-75Ws / ml; ukucindezela okukhulu.
Incomo yedivayisi:
I-UIP2000hd
Isikhombo / Iphepha Lokucwaninga:
I-Vilkhu, K .; UManase, R .; Mawson, R .; U-Ashokkumar, M. (2011): Ukubuyiswa kwe-ultrasonic nokuguqulwa kokudla izithako. Ku: Feng / Barbosa-Cánovas / Weiss (2011): Ubuchwepheshe be-Ultrasound for Food and Bioprocessing. I-New York: Springer, 2011. iphe. 345-368.

I-Polyphenols, i-amino acid ne-caffeine kusuka etizeni eluhlaza

Isicelo se-Ultrasonic:
Ukwenziwa kwamakhemikhali asebenzayo avela eThekwini elihlaza emanzini
Incomo yedivayisi:
UP400S

Ingulube: Ukugcoba kwezingulube ezinqeni

Isicelo se-Ultrasonic:
Ukushiswa kwe-ultrasonically, ingulube yezinkukhu (i-Longissimus dorsi) igxiliswe kwi-sodium chloride brine (40 g L-1) futhi iphathwa ngo-5 ° C nge-low-frequency ultrasound (20 kHz) ngokuphansi (2-4 W / cm -2). Umphumela we-ultrasonic wasiza ekuphulukiseni i-microstructure ezincane, amaprotheni denaturation, amandla okubopha amanzi (WBC), amandla okubamba amanzi (i-WHC), i-sodium chloride diffusion coefficient (D) kanye nenyama ye-textural profile (TPA) yophenyo. Imiphumela yabonisa ukuthi ukwelashwa kwe-ultrasonic kwabangele ushintsho oluthile lwezingqalasizinda ezingxenyeni zenyama. Amandla okubamba amanzi kanye nezakhiwo zombhalo zithuthukiswa ukwelashwa kwe-ultrasonic uma kuqhathaniswa namasampula aphelile aphelile. Kodwa-ke, le miphumela emihle yayixhomeke kakhulu ekutheni amandla e-ultrasonic agxile kakhulu. Amandla aphezulu kanye / noma izikhathi zokwelashwa ezinde kwaholela ekudaleni ama-protein. I-model ye-diffusion coefficient yakwazi ukuchaza ngokunembile i-NaCl diffusion kinetics ngenkathi ibhaka. Ukwelashwa kwe-Ultrasonic kwandisa kakhulu ukusabalala kosawoti uma kuqhathaniswa namasampuli ahlanganiswe ngaphansi kwezimo eziqinile kanye nokwehlukaniswa kwamakhemikhali okwandiswa ngokukhula okukhulu kwe-ultrasonic.
Incomo yedivayisi:
UP200Ht nge sonotrode S26d40
Isikhombo / Iphepha Lokucwaninga:
I-Siro, I .; I-Ven, i-Cs; I-Balla, i-Cs; UJonas, uG .; Zeke, I .; UFriedrich, L. (2009): Isicelo se-ultrasonic esasiza ekwelapheni inqubo yokuthuthukisa ukusabalalisa kwe-sodium chloride emzimbeni wenyamazane. Journal of Food Engineering 91/2, 2009. 353-362.

Porphyra yezoensis

Isicelo se-Ultrasonic:
Ukuchithwa kwe-ultrasonic yama-polysaccharides avela ku-Porphyra yezoensis: isisombululo se-porphyra yezoensis polysaccarides (isisindo esomile) esingu-50mL se-50mL senziwe nge-hr 4 nge-UP400S.
Incomo yedivayisi:
UP400S; i-pulsed ultrasound (imijikelezo: i-2 sec kuse / 2 sec isekhondi) ku-20 ° C.

Amandla

Isicelo se-Ultrasonic:
Ukugaya, ukuguqula nokusabalalisa nokusabalalisa obuncane be-particativley usayizi wezinhlayiyana.
Incomo yedivayisi:
UP200St

Amathambo om Rat

Isicelo se-Ultrasonic:
Ukuphazanyiswa kwe-1 gm ku-5-7 iminithi.
Incomo yedivayisi:
UP200St

Isibindi sesibindi

Isicelo se-Ultrasonic:
Ukuphazanyiswa kanye ne-tissu homogenization nge-UP400S.
Incomo yedivayisi:
UP400S; Izikhathi ezingu-3 ngomzuzwana wama-30; phezu kweqhwa.
Isikhombo / Iphepha Lokucwaninga:
Oh et al. (2003): I-Acetyl-CoA I-Carboxylase Gene ilawulwa yi-Protein-1 ebophezelayo i-Protein-1 e-Liver.

Isikhumba sesikhumba

Isicelo se-Ultrasonic:
Ukuphazamiseka kwe-1 gm kumaminithi angu-1.
Incomo yedivayisi:
UP400S

Rawolfia Serpentina

Isicelo se-Ultrasonic:
Ukukhishwa kwe-alkaloid reserpine.
Incomo yedivayisi:
UP100H

I-rebadiodioside A

Isicelo se-Ultrasonic:
Ama-sampuli ama-10g omile anama-stevia amaqabunga asemhlabathini athathwa ngamanzi angama-100mL ngaphansi kokugqugquzela okuqhubekayo (nge-stirrer magnetic). I-pH value yayilawulwa nge-0.01 M pH 7 ye-phosphate ye-sodium. Isampula yayifakwe ku-glass beaker eyi-150 ml kanye ne-ultrasonicator ye-probe-type (UIP500hd, 20kHz, 500W). Iphuzu le-sonotrode labhapathizwa cishe ngo-1.5 cm kuya emagqabini ase-stevia. Idivayisi ye-ultrasonic isethelwe amandla okukhishwa kwe-350W. Ukwelashwa okunomusa kwe-350 W kwe-5-10 min. ekushiseni okuqhubekayo kwama-30 ° C wanikezela i-rebaudioside A isivuno se-30-34g ngesampula se-100g. Ngemuva kwe-sonication, ikhambi lokukhipha laliyi-centrifuged futhi lihlungiwe ngo-0.45 μm ulwelwesi lwe-microporous; i-filtrate ithathwe ukuhlaziywa kokuqukethwe kwe-rebaudioside. Ukukhiqizwa kokukhipha kwe-rebaudioside A okuphelele okuqukethwe kwahlaziywa yi-HPLC.
Nge-ultrasound-assisted isizinda esingenaso solvent-free, isivuno esikhulu se-rebaudioside A satholwa ngokuqhathaniswa nezindlela zendabuko yesikhumba ezifana nokushisa kwesakhiwo noma i-maceration.
Incomo yedivayisi:
UIP500hd

I-Rice starch

Isicelo se-Ultrasonic:
Ukuphazanyiswa, isitashi ukuhlukaniswa, nokuphulwa kwezibopho ezingekho emthethweni phakathi kwamaprotheni nesitashi emfucumfuceni welayisi (33%) ngo-20 – 40 iminithi.
Incomo yedivayisi:
UP500hd; 20 – 40 iminithi.

Rotifers

Isicelo se-Ultrasonic:
Ukuphazanyiswa kwamagciwane e-mesozooplankton: ngokufaka ingane ngaphansi kwezimo ezine zezinga lokugeleza (200, 400, 520 no-800lh-1) kanye nama-amplitudes amane (25, 50, 75 no-100%) izinga lokubulala phakathi kuka-58 no-85% liye lafezwa.
Incomo yedivayisi:
UP2000 nge-flow cell
Isikhombo / Iphepha Lokucwaninga:
Viitaslo et al. (2005): i-Ozone, i-Ultraviolet Light, i-Ultrasound ne-Hydrogen Peroxide Njengoba i-Ballast Water Treatments – Ukuhlola nge-Mesozooplankton E-Low Saline- Amanzi E-Brackish.

S. fragilis

Isicelo se-Ultrasonic:
ukukhululwa kwe-galactokinease kumaminithi angu-4.
Incomo yedivayisi:
UP200St

Saccharomyces cerevisiae

Isicelo se-Ultrasonic:
Ukuphazanyiswa
Incomo yedivayisi:
UP400S
Isikhombo / Iphepha Lokucwaninga:
Guerrero, S ,; López-Malo, A .; Alzamora, SM (2001): Imiphumela ye-ultrasound ekusindeni kwe-Saccharomyces cerevisiae: ithonya lokushisa, i-pH ne-amplitude. Innov. Ukudla Sci. Emerg Technol. 2/2001. k. 31-39.

Saccharomyces cerevisiae

Isicelo se-Ultrasonic:
Inactivation ye-Ultrasonic ye-Saccharomyces cerevisiae eSabouraud ukukhula okuphakathi kanye no-saline.
Incomo yedivayisi:
UP400S
Isikhombo / Iphepha Lokucwaninga:
I-Yap, A .; UJiranek, uV .; Grbin, P .; Barnes, M .; Bates, D. (2007): Izifundo ekusetshenzisweni kwamandla amakhulu e-ultrasonics emigodini nokuhlanzwa kweplanethi nokucwiliswa kwe-disinfection. I-Australiya. I-NZ Wine Indust. J. 22 (3) / 2007. iphe. 96-104.

I-Salmonella Senftenberg 775W Gr-

Isicelo se-Ultrasonic:
Ukusetshenziswa kwe-Ultrasonic yeSalmonella Senftenberg 775W Gr- kuMcIlvaine citrate-phosphate buffer noma umhluzi wezakhi.
Incomo yedivayisi:
UP100H
Isikhombo / Iphepha Lokucwaninga:
Álvarez, I .; I-Manas, i-P .; I-Virto, R .; Condón, S. (2006): Ukungasebenzi kwe-Salmonella Senftenberg 775W ngamagagasi e-ultrasonic ngaphansi kwengcindezi emisebenzini yamanzi ehlukene. Int. J. Food Microbiol. 108/2006. iphe. 218-225.

Salvia miltiorrhiza bunge

Isicelo se-Ultrasonic:
Ukwenziwa kwamakhemikhali asebenzayo: i-Dansensu ye-sodium ne-four tanshinones (i-dihydrotanshione I, tanshinone I, cryptotanshinone ne-tanshinone IIA).
Incomo yedivayisi:
UP100H

I-Salvia Officinalis

Isicelo se-Ultrasonic:
Ukwenziwa kwamakhemikhali asebenzayo avela kuSalvia Officinalis (inhlakanipho) engaphansi kwe-2hr.
Incomo yedivayisi:
UP50H

I-Serum

Isicelo se-Ultrasonic:
i-homogenization
Incomo yedivayisi:
UP200Ht

Izikhumba ze-Shes cystic, amaphaphu kanye negazi elihle (i-Echinococcus granulosus antigens)

Isicelo se-Ultrasonic:
Izikhumba ze-cystic zezimvu, amaphaphu kanye negazi elihle (i-Echinococcus granulosus antigens ngamawundlu): Isampula yabe isetshenziselwa i-2 × 15 sec eqhweni kuze kube yilapho kungekho ama-protoscolic aqinile.
Incomo yedivayisi:
UP200S; 2 x 15 sec.
Isikhombo / Iphepha Lokucwaninga:
I-Tabar et al. (2009): Ukusabela kwe-Antibody ngokumelene ne-hydatid fluid, i-protoscolex nomzimba wonke we-Echinococcus granulosus antigens ngamawundlu.

I-Sorbitant Trioleate, i-ethanol (njenge-solvent) ne-Bi-2212 powder

Isicelo se-Ultrasonic:
Hlanganisa i-slurry equkethe i-dispersant Sorbitant Trioleate, i-ethanol (njenge-solvent) ne-Bi-2212 powder.
Incomo yedivayisi:
UP200S; imizuzu engu-3.
Isikhombo / Iphepha Lokucwaninga:
Mora et al. (2009): Ukufakelwa kwama-Superconducting Coatings kumaTelamic Tiles esakhiweni.

I-Soy isoflavones

Isicelo se-Ultrasonic:
Isizinda se-ultrasonic se-soy isoflavones emanzini nase-solvent. Ukuvunwa okwenyukayo kufinyelele ku-15% ekusebenzeni kokukhipha.
Incomo yedivayisi:
UP200St
Isikhombo / Iphepha Lokucwaninga:
Rostagno et al. (2003), okukhulunywa nguVilkhu, K .; UManase, R .; Mawson, R .; U-Ashokkumar, M. (2011): Ukubuyiswa kwe-ultrasonic nokuguqulwa kokudla izithako. Ku: Feng / Barbosa-Cánovas / Weiss (2011): Ubuchwepheshe be-Ultrasound for Food and Bioprocessing. I-New York: Springer, 2011. iphe. 345-368.

Soy amaprotheni

Isicelo se-Ultrasonic:
Isizinda se-ultrasonic seprotheni ye-soy emanzini nase-alkali (i-hydrodium i-sodium). Ukuvunwa okwandisiwe ngo-53%. I-inication sonication itholwe ngokuphumelelayo njengokwakheka kwe-batch (sonication encane inikeze isivuno esingaphezulu kuka-23% kune-sonication ye-batch).
Incomo yedivayisi:
I-UIP1000hd for batch / beaker kanye ne-flow cell reactor for sonication encane.
Isikhombo / Iphepha Lokucwaninga:
UMoulton noWang (1982), okukhulunywe nguVilkhu, K .; UManase, R .; Mawson, R .; U-Ashokkumar, M. (2011): Ukubuyiswa kwe-ultrasonic nokuguqulwa kokudla izithako. Ku: Feng / Barbosa-Cánovas / Weiss (2011): Ubuchwepheshe be-Ultrasound for Food and Bioprocessing. I-New York: Springer, 2011. iphe. 345-368.

Izinhloko ze-sperm (umuntu)

Isicelo se-Ultrasonic:
Ukuphazamiseka kumaminithi angu-10.
Incomo yedivayisi:
UP100H

I-sperm imisila (umuntu)

Isicelo se-Ultrasonic:
Ukuphazanyiswa ngokushesha
Incomo yedivayisi:
UP100H

Stevia rebaudiana Bert.

Isicelo se-Ultrasonic:
Isizinda se-Ultrasonic se-stevioside glycoside kusuka kumasampula ka-10 g wamaqabunga omile avela ku-Stevia rebaudiana enezinhlayiya ezine eziyingqimba (0.315 mm, 2 mm, 6.3 mm kanye namaqabunga aphelile) axutshwe nama-solvents ahlukene: amanzi ahlanzekile namanzi / ethanol (55% kanye no-70%) nesisampuli esilinganiselwe esilinganisweni sokulinganisa ivolumu: 1/10, 1/8, 1/5 (w / v) bese kuvezwa i-ultrasound ekamelweni lokushisa. Isikhathi se-Sonication: < 5 min. Incomo yedivayisi:
UP200St