ICoronavirus (COVID-19, SARS-CoV-2) ne-Ultrasonics I-Ultrasonication iyithuluzi elinamandla elisetshenziswa ku-biology, chemistry chemistry kanye ne-biochemistry nasekukhiqizweni kwemithi yemithi. Ama-biio-science asebenzisa ama-homogenizer e-ultrasonic ukwenza ama-lyse cell futhi akhiphe amaprotheni nezinye izinto ezisetshenziswayo, imboni yama-pharma isebenzisa ama-ultrasonics ekwakheni ama-molecule asebenza emakhemikhali, ukukhiqiza imigomo futhi ibenze babe ngabathwali bezidakamizwa ababi nano. Ngesikhathi sokulwa nenoveli coronavirus SARS-CoV-2 ultrasonicators asetshenziselwa izinhlelo ezahlukahlukene ocwaningweni, i-bio-science ne pharma. I-Ultrasonication for Development and Production of Pharmaceuticals Synthesis of Pharmacologic Active Molecule Kuthuthukisiwe Remdesivir Solubility ngu Sonication I-Ultrasonic Synthesis yeChoroquine ne-Analogues Ukukhishwa kwe-Ultrasonic yezinto eziphilayo ze-Bioactive kusuka ku-Botanicals I-Ultrasonic Vaccine Production Izicelo ze-Ultrasonic ze-Vaccine Production Ukwenza Ukuthuthukiswa Kwe-Vaccine Ngamandla Nge-Ultrasound Ukukhiqizwa kwemithi yokugoma ye-RNA nge-Ultrasonics Ukwenziwa kwe-Ultrasonic Kwemithi Ultrasonic Liposome Ukulungiselela Ukukhiqizwa kwe-Ultrasonic kweVitamin C Liposomes Ukukhiqizwa kwe-Ultrasonic kweSolid Lipid Nanoparticles Ukulungiswa kwe-Ultrasonic kweCyclodextrin Complexes Ivermection-Loaded Solid-Lipid Nanoparticles nge Sonication I-Ultrasonic Nano-Emulsification I-Ultrasonic Nano-Emulsification ye-Microencapsulation ngaphambi kokufiphaza-Ukumisa Ukuncishiswa kwe-Ultrasonic Viscosity ngaphambi kokufafaza Igciwane Isicelo solwazi Igama Ikheli le-imeyili (elidingekayo) umkhiqizo noma indawo yesithakazelo Qaphela kwethu Inqubomgomo yobumfihlo. Cela ulwazi I-Ultrasonics yokucwaninga ku-Bio-Science ne-Bio-Chemistry Ukuphazamiseka kweseli ye-Ultrasonic, i-Lysis kanye ne-Extraction I-Ultrasonic DNA kanye ne-RNA Shearing I-Ultrasonic Lysis ye-Western Blotting I-Ultrasonics ekucwaningweni kwegciwane Ama-Ultrasonicators aphezulu okusebenza kwe-Pharma ne-Bio-Science Izinhlelo ze-Hielscher Ultrasonics 'zisetshenziselwa kabanzi ekwenziweni kwemithi ukuhlanganisa ama-molecule asezingeni eliphakeme futhi kwakhiwe ama-nanoparticles e-lipid aqinile kanye nama-liposomes athwala izinto zemithi, amavithamini, ama-antioxidants, ama-peptides nezinye izinto ezixubile. Ukuhlangabezana nezidingo zamakhasimende ayo, ama-Hielscher anikezela ngama-ultrasonicators avela ku-compact, nokho ama-hand homogenizer aphethwe ngesandla anamandla aphezulu okubeka ezinhlelweni ze-ultrasonic zezimboni ngokugcwele zokukhiqiza izimfanelo eziphakeme zezinto zokwenziwa kwemithi nokwakhiwa. Uhla olubanzi lwama-sonotrodes we-ultrasonic kanye nama-reactors atholakalayo ukuqinisekisa ukusethwa okuphezulu komkhiqizo wakho wemithi. Ukuba namandla kwemishini yokusebenza kwe-Hielscher sika-Hielscher kuvumela ukusebenza kwe-24/7 emsebenzini osindayo nasezindaweni ezifunwayo. Ukuze uvumele amakhasimende ethu ukuthi afeze i-Good yokukhiqiza imisebenzi (i-GMP) futhi asungule izinqubo ezihleliwe, wonke ama-ultrasonicators edijithali afakwe isoftware ehlakaniphile yokusetha ngokunembile ipharamitha ye-sonication, ukulawulwa kwenqubo okuqhubekayo nokuqoshwa okuzenzakalelayo kwawo wonke amapharamitha wenqubo abalulekile esakhiweni -Kukhadi le-SD. Ikhwalithi ephezulu yomkhiqizo incike ekulawulweni kwenqubo nakumazinga wokuphakama aphezulu ngokuqhubekayo. Ama-ultrasonicators we-Hielscher akusiza ukuthi uqaphele futhi wenze inqubo yakho ibe ng linye! Iyunithi yokulungiselela isampula ye-Ultrasonic I-VialTweeter: I-sonline ye-VialTweeter ku-processor ye-ultrasonic UP200St Ukulinganisa Inani eliphakeme lamacala e-COVID-19 ayinselelo enkulu ohlelweni lwezempilo kubandakanya nokucwaninga nokukhiqizwa kwezemithi. Ngenkathi okwamanje kunezinto eziningi zemithi ezingaphansi kophenyo (in vitro and vivo), kusukela manje sekutholakele ukwelashwa kweziguli ze-COVID-19, inani elikhulu lezidakamizwa kumele likhiqizwe esikhathini esifushane. Ukuhlanganiswa kwe-ultrasonic kwe-chloroquine ne-chloroquine kuyinqubo esheshayo, elula nephephile, engalinganiswa ngokuqondile kusuka kwelebhu nesitshalo somshayeli kuya ekukhiqizeni okugcwele okuthengiswayo. Abasebenzi bethu abaqeqeshiwe nabanesipiliyoni eside bazokusiza ngobuchwepheshe kusuka ekuvivinyweni komshayeli kuya ekukhiqizweni kwenani elikhulu. Ithebula elingezansi linikeza isibonakaliso somthamo wokucubungula we-ultrasonicators wethu: Iqoqwana Ivolumu flow Rate Amadivayisi ezinconyiwe 1 kuya ku-500mL 10 kuya ku-200mL / min UP100H 10 kuya ku-2000mL 20 kuya ku-400mL / min UP200Ht, UP400St 0.1 kuya ku-20L 0.2 kuya ku-4L / min I-UIP2000hdT 10 kuya ku-100L 2 kuya ku-10L / iminithi I-UIP4000hdT na 10 kuya ku-100L / iminithi UIP16000 na sikhulu yinhlanganisela UIP16000 Xhumana nathi! / Cela Us! Cela ulwazi oluthe xaxa Uyacelwa ukuthi usebenzise ifomu elingezansi ukucela ulwazi olungeziwe mayelana nezinhlelo zokusebenza ze-ultrasonic, izinhlelo zokusebenza nentengo. Sizokujabulela ukuxoxa ngenqubo yakho nawe futhi sikunikeze uhlelo lwe-ultrasonic oluhlangabezana nezidingo zakho! Igama Inkampani Ikheli le-imeyili (elidingekayo) Inombolo yocingo Ikheli Idolobha, i-State, i-ZIP Code Izwe Inzalo Sicela uqaphele wethu Inqubomgomo yobumfihlo. Cela ulwazi Amandla aphezulu we-homogenizer ephezulu I-Lab kuya umshayeli futhi Izimboni isikali. Izincwadi / Izinkomba Mohammad Owais, Grish C. Varshney, Amit Choudhury, Subhash Chandra, Chitar M. Gupta (1995): I-Chloroquine Ihlanganiswe eMalariya Etheleleke nge-Erythrocyte-Specific Antibody-Bearing Liposomes ngempumelelo I-Chloroquine- Resistant Plasmodium berghei ukutheleleka eMice. Ama-antimicrobial Agents ne-Chemotherapy, Jan. 1995. 180-184. U-Shah Purvin, Parameswara Rao Vuddanda, Sanjay Kumar Singh, Achint Jain, noSanjay Singh (2014): I-Pharmacokinetic neTissue Distribution Study ye-Solid Lipid Nanoparticles ye-Zidovudine ku-Rats. Ijenali yeNanotechnology, uMqulu 2014. UJoanna Kopecka, uGiuseppina Salzano, u-PharmDa, u-Ivana Campia, uSara Lusa, uDario Ghigo, uGiuseppe De Rosa, uChiara Riganti (2013): Imininingwane engxenyeni yamakhemikhali e-liposomes ebhekene nokuvinjwa kwe-P-glycoprotein. I-Nanomedicine: I-Nanotechnology, i-Biology, ne-Medicine 2013. IHarshita Krishnatreyya, Sanjay Dey, Paulami Pal, Pranab Jyoti Das, Vipin Kumar Sharma, Bhaskar Mazumder (2019): I-Piroxicam Loaded Solid Lipid Nanoparticles (SLNs): Okungabakhona Kokulethwa Okuphezulu. I-Indian Journal ye-Pharmaceutical Education and Research Vol 53, Isikhombi 2, 2019. 82-92. Okuthunyelwe okuhlobene ILiposomal Vitamin C Production ne-Ultrasonics Amakhemikhali Ahlanganiswe eLipid Nanoparticles nge-Ultrasonics I-Ultrasonic Synthesis ye-Pharma-grade Chloroquine I-Ultrasonically Ihlelwe i-Famotidine Solid-Lipid Nanoparticles I-Liposomal encapsured Bioactive Molecule enama-Ultrasonics Ama-Nootropics kanye nama-Smart Drug formulations ane-Ultrasonics Amaqiniso Okufanele Ukwazi I-SARS-CoV-2 I-SARS-CoV-2 coronavirus, eyaziwa nangokuthi yi-2019-nCoV noma i-novel coronavirus 2019, inesibopho sengozi yobhubhane i-COVID-19, eyaqala ngoZibandlela wezi-2019 eWuhan, eChina futhi yasakazekela lapho wonke umhlaba. Ngokwesilinganiso sokutheleleka okuphezulu / izinga lokudlulisela, abakwaSARS-CoV-2 basakazeka ikakhulu ngokutheleleka nge-droplet kanye nokudluliselwa kwefomite. Kodwa-ke, njengoba izinhlayiya zegciwane zingatholakala futhi ezindlebeni, ukudluliswa komgudu wokulinganisa ngomlomo kungenzeka, nakho. Indlela enkulu yokudluliselwa komuntu kumuntu nge-SARS-CoV-2 ukuxhumana nabantu abasondelethekile: Amaconsi wokuphefumula akhiqizwa ngokunyenya nokukhwehlela komuntu onegciwane kudonswa abanye, ukuze kamuva atheleleke. Ama-Coronavirus anjenge-SARS-CoV-2 anamathela ku-angiotensin-converting enzyme 2 (ACE2), atholakala kakhulu emaphashini (futhi ngezinga elincane enhliziyweni, emathunjini, emithanjeni nasezinso). Amaprotheni e-coronavirus spike (S-protein / glycoproteins), aphuma emvilophini ye-coronavirus, abopha i-ACE2 receptor, ahlangane ne-membrane ye-host cell bese engena ngale ndlela yeseli eliphethe. Njengawo wonke ama-virus, ama-coronaviruse asebenzisa iseli eliyisebenzisayo ukuphinda i-genome yawo futhi enze ngayo izinhlayiya ezintsha zegciwane. Ama-Coronaviruses aqukethe i-genNA eqondile, enomugqa owodwa. Ngokungafani namagciwane omkhuhlane, i-coronavirus yigciwane elingabaliwe. I-Sars-CoV-2 ine-genome emfushane eyenziwe ngentambo eyodwa nje ende yamangqamuzana zofuzo. Lokhu kusho ukuthi amagciwane we-SARS-CoV-2 aqukethe ingxenye eyodwa, kuphela. Amagciwane omkhuhlane, angamagciwane we-RNA afana nama-coronavirus, anezakhi zofuzo ezihlukaniswe ngezigaba eziyisishiyagalombili zofuzo. Lokhu kunikeza igciwane lomkhuhlane ikhono elikhethekile lokuphinda lihlanganise / ukuguqula izakhi zofuzo. Ama-Coronaviruses Igama lesayensi le-coronavirus yi-Orthocoronavirinae noma iCoronavirinae, iCoronavirus ingeyomndeni wakwaCoronaviridae. Ama-Coronaviruses yiqembu lamagciwane ahlobene adala izifo ezincelisayo nasezinyoni. Ngokwesibalo sabantu, ukutheleleka kwe-coronavirus kuphumela ekuthelelekeni kwezifo zokuphefumula. Ukutheleleka kwezifo zokuphefumula okunjalo kungaba nemiphumela emincane, kuvezwe njengamakhaza ajwayelekile (isib. Obhejane), kanti ezinye izifo ze-coronavirus zingabulala, njenge-SARS (Severe Acute Respiratory Syndrome), i-MERS (Middle East Respiratory Syndrome), ne-COVID-19 (iCoronavirus) Izifo 2019). Ama-Coronaviruses wesintu Mayelana nama-coronavirus esintu, izinhlobo eziyisikhombisa ziyaziwa. Ezine zalezi zinto eziyisikhombisa ze-coronavirus zivusa izimpawu ezithambile ngokuvamile, ezaziwa ngokuthi umkhuhlane ovamile: I-human coronavirus OC43 (HCoV-OC43) I-human coronavirus HKU1 I-human coronavirus NL63 (HCoV-NL63, i-New Haven coronavirus) I-human coronavirus 229E (HCoV-229E) Ama-coronaviruses HCoV-229E, -NL63, -OC43, kanye -HKU1 ajikeleza ngokuphelele inani labantu futhi abangela izifo eziphefumula eziphakathi nendawo kubantu abadala nezingane emhlabeni jikelele. Kodwa-ke, izinhlobo ezintathu ze-coronavirus ezingezansi ziyaziwa ngezimpawu zazo ezinzima: I-coronavirus ehlobene ne-Middle East yokuphefumula i-coronavirus (MERS-CoV), eyaziwa nangokuthi yi-novel coronavirus 2012 ne-HCoV-EMC I-corvevirus enamandla ye-acute acute yokuphefumula (i-SARS-CoV / SARS-classic) I-acute acute respiratory syndrome coronavirus 2 (SARS-CoV-2), eyaziwa nangokuthi yi-2019-nCoV noma i-novelos coronavirus 2019
Izincwadi / Izinkomba Mohammad Owais, Grish C. Varshney, Amit Choudhury, Subhash Chandra, Chitar M. Gupta (1995): I-Chloroquine Ihlanganiswe eMalariya Etheleleke nge-Erythrocyte-Specific Antibody-Bearing Liposomes ngempumelelo I-Chloroquine- Resistant Plasmodium berghei ukutheleleka eMice. Ama-antimicrobial Agents ne-Chemotherapy, Jan. 1995. 180-184. U-Shah Purvin, Parameswara Rao Vuddanda, Sanjay Kumar Singh, Achint Jain, noSanjay Singh (2014): I-Pharmacokinetic neTissue Distribution Study ye-Solid Lipid Nanoparticles ye-Zidovudine ku-Rats. Ijenali yeNanotechnology, uMqulu 2014. UJoanna Kopecka, uGiuseppina Salzano, u-PharmDa, u-Ivana Campia, uSara Lusa, uDario Ghigo, uGiuseppe De Rosa, uChiara Riganti (2013): Imininingwane engxenyeni yamakhemikhali e-liposomes ebhekene nokuvinjwa kwe-P-glycoprotein. I-Nanomedicine: I-Nanotechnology, i-Biology, ne-Medicine 2013. IHarshita Krishnatreyya, Sanjay Dey, Paulami Pal, Pranab Jyoti Das, Vipin Kumar Sharma, Bhaskar Mazumder (2019): I-Piroxicam Loaded Solid Lipid Nanoparticles (SLNs): Okungabakhona Kokulethwa Okuphezulu. I-Indian Journal ye-Pharmaceutical Education and Research Vol 53, Isikhombi 2, 2019. 82-92.