I-Hielscher Ultrasound Technology

I-Algae Landa Lab – Ultrasonic Algae Extraction

Ukulimala kwe-Algae

I-Algae Grow Lab yenza uchungechunge lwama-photobioreactors we-tubular ne-flat for algae ukutshalwa kanye nenqubo yesimiso se-ultrasonic esekelwe kuma-processors we-Hielscher ultrasonic afakwe ukugeleza kwamaseli.
Umdwebo jikelele wokugeleza kwenqubo uboniswa ngezansi.

I-Algae Gro Lab yaqala ukusetha inqubo ephelele kuhlanganise ne-bioreactor yokutshala kwe-algae kanye nokusebenza okuphansi ukuthola amafutha algae.

Ishadi lokugeleza libonisa inqubo yokutshala i-algae nokukhiqizwa kwamafutha algal usebenzisa i-ultrasonication. © I-Algae Grow Lab

Izibonelo ze-Algae Ukukhulisa i-Labbibibi zenzelwe ngezansi.
Ukusetshenziswa kwamapaneli e-LED aveza ukukhanya ku-PAR ingxenye ye-spectrum ivumela ukufezekisa izinga eliphezulu lokukhula kwe-algae.
Isibonelo, ngemuva kwe-inoculation ye-Chlorella Vulgaris enobukhulu bokuqala buka-0.146 g / L sithole ukuphakama kwe-7.3g / L ezinsukwini ezingu-7.

www.algaegrowlab.com

I-Algae Grow Lab inikeza izithombe-bioreactors nemishini yokukhiqiza amafutha alge.

Ama-Algae Amaseli Ukubhujiswa ngu-Ultrasonification

Ngemuva kwenkundla yokukhula kwe-algae, i-algae cell isetshenziselwa ukwelashwa kwamafutha. Njengoba okuqukethwe kwe-cell kuhlukaniswa nemvelo ehambile ngesakhiwo sezingqamuzana zamangqamuzana, indlela yokuphazamiseka kwamangqamuzana ibalulekile ngokukhishwa kwezinto eziphelele ze-intracellular. I-membrane yeseli inikeza amandla omzimba esitokisini futhi igcine ubuqotho bayo. Izakhiwo ezinwetshiwe zekhanda lesitokisi livumela amangqamuzana ukuthi alwe nezinguquko ezisheshayo ezingcindezini ze-osmotic ezingase zenzeke endaweni yazo zangaphandle.
Zombili izindlela ze-ultrasound nezindlela ezisizwa nge-microwave, ezichazwe ngezansi, zithuthukisa kakhulu ukukhishwa kwe-microalgae kakhulu, ngokusebenza kahle, ukunciphisa izikhathi zokukhipha kanye nokwenyuka kwezivuno, kanye nezindleko eziphansi kuya ezilinganisweni kanye nokwehliswa okungekho emthethweni.
Ngokuvamile ukukhishwa kwemikhiqizo yemigomo evela ku-algae kuyasebenza kakhulu uma amaseli algae ebhujiswa ngaphambi kokukhipha. Kodwa ngezinye izikhathi, ukubhujiswa kwamangqamuzana ngokwayo kuholela ekukhululweni komkhiqizo womgomo, kanti kuphela ukuhlukaniswa okudingekayo ukuze kutholakale (isib. Ukukhishwa kwe-lipids ku-algae yokukhiqizwa kwe-biofuel).
I-Algae ikhula ilabhu ihlanganisa uhlelo lwe-ultrasonic lwe-cell ukuphazanyiswa kanye ne-extraction ekusethweni kwabo ukuqinisekisa ukuthi inqubo ephumelelayo kakhulu ekufinyeleleni ukukhululwa okuphelele kwe-intracellular okuqukethwe futhi ngaleyo ndlela izithelo eziphakeme ngesikhathi esifushane. Esikhathini se-reactor ultrasonic, amagagasi e-ultrasonic enza i-caviatation emithonjeni ye-liquid equkethe amaseli algae. I-bubble cavitation ikhula ngesikhathi sezingxenyana ezingezansi ze-ultrasonic kuze kube yilapho zifinyelela usayizi othize, lapho kungekho amandla angaphezulu angasakazwa nge-adsorbed. Kuleli phuzu eliphakeme lokukhula kwe-bubble, i-voids iyawa ngesikhathi sesigaba sokucindezela. Ukuwa kwe-bubble kudala izimo ezimbi kakhulu zokucindezela nokushisa kanye namaza okushaqeka kanye nama-jets ayenamandla kagesi. Lezi zimpi ezimbi kakhulu azigcini nje kuphela ukubhubhisa amaseli, kodwa futhi zihlanza kahle okuqukethwe kwazo emithonjeni yamanzi (isb. Amanzi noma i-solvents).
Ukuphumelela kokubhujiswa kwe-ultrasonic kuncike ngokuqinile ekuqineni nasekuqineni kwezindonga zezingqamuzana, ezihluka kakhulu phakathi kwezinkinga ze-algae ngayinye. Yingakho i-efficieny yokubhujiswa kwamangqamuzana ithonywa kakhulu yimingcele yenqubo yokufaka indodana: Imingcele ebaluleke kakhulu yi-amplitude, ingcindezi, ukuhlushwa & i-viscosity, nokushisa. Lezi ziminganiso kumele zilungiselelwe zonke izinhlobo ezithile ze-algae ukuqinisekisa ukuthi ukusebenza kahle kokusebenza kahle.
Ezinye izibonelo ze-cell ukuphazanyiswa nokuhlukaniswa kwezinkinga ezihlukahlukene ze-algae zingatholakala ezihlokweni ezibalulwe ngezansi:

  • UDunnaliella salina noNannochloropsis oculata: INkosi PM, i-Nowotarski K .; Joyce, EM; Mason, TJ (2012): Ukuphazanyiswa kwe-Ultrasonic kwamaseli algae. I-AIP Conference Proceedings; 5/24/2012, Umq. 1433 Issue 1, k. 237.
  • Nannochloropsis oculata: uJonathan R. McMillan, u-Ian A. Watson, uMehmood Ali, Weaam Jaafar (2013): Ukuhlola nokuqhathanisa izindlela ze-algal cell ukuphazanyiswa: Microwave, amanzibath, blender, ultrasonic kanye nelase laser. I-Applied Energy, ngoMashi 2013, Umq. 103, amakhasi 128-134.
  • I-Nanochloropsis salina: Sebastian Schwede, Alexandra Kowalczyk, Mandy Gerber, Roland Span (2011): Ithonya lamasu ahlukahlukene okuphazanyiswa kwamaseli ku-mono ukumiswa kwe-algal biomass. I-World Renewable Energy Congress 2011, i-Bioenergy Technologies, ngo-8-12 Meyi 2011, eSweden.
  • I-Schizochytrium limacinum ne-Chlamydomonas reinhardtii: Jose Gerde, Mellissa Montalbo-Lomboy M, Linxing Yao, David Grewell, Tong Wang (2012): Ukuhlolwa kwe-cellalgae cell ukuphazanyiswa ngokuphathwa kwe-ultrasonic. I-Bioresource Technology 2012, Umq. 125, pp.175-81.
  • I-Crypthecodinium cohnii: Paula Mercer noRoberto E. Armenta (2011): Ukuthuthukiswa kokukhipha amafutha kusuka ku-microalgae. I-Europeen Jornal ye-Lipid Science Technology, ngo-2011.
  • I-Scotiellopsis terrestris: uS. S.ke, uDkt. N. Hempel, uL. Dombrowski, uProf. Dr. O. Pulz: Ukuthuthukiswa kwesisiphazamiso se-Scotiellopsis terrestris nge-ultrasound kanye ne-pectin e-enzyme ehlakazekile. Naturstoffchemie.
Ukulima kwe-algae ku-photo 500-bioreactor ye-500L

I-photolioreactor ye-tubular ye-500L enezintambo ze-LED © I-Algae Grow Lab

I-Algae Grow Lab inikeza ama-photo-bioreactors kumiklamo ehlukahlukene yokutshala i-algae.

Isithombe se-flat-bioreactor esinezikhala ze-LED © Algae Grow Lab

Inqubo

Ngemuva kokulima, i-algae biomass stream isondliwa kudivayisi yokuhlushwa ukuze ihlukanise i-biomass emithonjeni yamanzi. Ukugxila kunqwabelene esitokisini sokugcina. Ngemuva kokuhlukaniswa, amangqamuzana kumelwe aphazamiseke ukuze akhiphe amafutha nezinye izinto ezingaphakathi kwe-intracellular. Ngakho-ke, i-biomass egxilile iqhutshwa ngedivaysi ye-Hielscher ultrasonic. Ukusethwa kwe-recycleulation ultrasonic kuqinisekisa ukuphindaphinda kwe-cell concentrate ngaphansi kwengcindezi enikeziwe ngokusebenzisa i-Hielscher flow flow cell emuva kwe-tank yokuqoqa. I-recirculation igcina isikhathi esidingekayo sokubhubhisa amaseli. Lapho inqubo yokubhujiswa isuphelile, i-biomass namaseli abhubhisayo iqhuma kumshini wokuhlukanisa umkhiqizo, lapho ukuhlukaniswa kokugcina komkhiqizo kusuka kumabhrikhsi asele kwenzeka.

I-ultrasonication enamandla iyindlela ephumelelayo yokwehlukaniswa kwamaseli algae. I-UIP1500hd ye-Hielscher i-homogenizer ye-watts engu-1500 engama-watts engahlanganiswa kalula ekusebenzeni okugcwele okufisa.

I-Algae cell destruction unit ene-biomass yokuhlushwa / idivaysi yokuhlukanisa kanye ne-Hielscher ka-1.5 kW iprosesa ye-ultrasonic i-UIP1500hd. © I-Algae Grow Lab

Ukukala kwephesenti yamaseli abhubhise

Ukuhlolwa kokusebenza kahle kwe-algae breakage, i-ALgae Grow Lab isebenzisa izindlela ezimbili ezihlukene zokulinganisa iphesenti yamaseli abhujisiwe:

  1. Indlela yokuqala yokuhlaziya isekelwe ekulinganisweni kwe-chlorophyll A, B, ne-A + B fluorescence.
    Ngesikhathi se-slow spin centrifugation, amangqamuzana e-algal nama-debris azofaka i-pellet ngaphansi komamukeli, kodwa ukuphumula kwe-chlorophyll ehamba phambili kuyobe kusalokhu kuhlale kuphezulu. Ukusebenzisa lezi zici zomzimba zeseli ne-chlorophyll, iphesenti lamaseli aphukile angatholakala. Lokhu kufezwa ngokulinganisa kuqala inani le-chlorophyll fluorescence yesampula. Khona-ke, isampula i-centrifuged. Emva kwalokho, i-fluorescence ye-chlorophyll ye-supernatant iyalinganiswa. Ngokuthatha iphesenti ye-chlorophyll fluorescence endaweni engaphezu kwe-fluoroshylene ye-chlorophyll yesampula esiphelele, ukulinganisela kumaphesenti wamaseli aphukile angenziwa. Leli fomu lokulinganisa linembile, kepha lenza ucabange ukuthi inombolo ye-chlorophyll ngayinye iyunifomu. Ingqikithi yenqwaba ye-chlorophyll yenziwa ngokusebenzisa i-methanol.
  2. Ngendlela yesibili yokuhlaziywa, i-hemocytometry ye-classic isetshenziselwe ukulinganisa isisindo somuntu esampula esikhalisiwe se-algae. Inqubo yenziwa ngezinyathelo ezimbili:
  • Okokuqala, isisindo samasampuli esampuli se-algae esivunyiwe ngaphambi kokuba ukwelashwa kwe-ultrasound kulinganiswa.
  • Okwesibili, inani lamaseli angabhubhiswanga (asele) ngemuva kokuzaliswa kwe-sampuli efanayo kufaniswa.
    Ngokusekelwe emiphumeleni yalezi zilinganiso ezimbili, iphesenti lamaseli abhujisiwe ibalwa.
Isithombe sibonisa ukugxila kwe-algae ngaphambi kokuphazanyiswa kweseli nge-power-ultrasound (i-Hielscher UIP1500hd). © I-Algae Grow Lab

I-Pic.1: I-Algae Ngaphambi Kokubhujiswa © I-Algae Grow Lab

Isithombe esincane kakhulu sikhombisa ukugxila kwe-algae ngemuva kwamaminithi angu-60. sonication. Ama-50% we-algae cell asevele ephukile.

I-Pic 2: Ukuphazanyiswa kwe-algae: 50% ukuphazanyiswa kwamaseli emva kwamaminithi angu-60. sonication. © I-Algae Grow Lab

Isithombe esincane se-ultrasonically siphazanyiswa futhi saphazamiseka amaseli algae. © I-Algae Grow Lab

I-Pic 3: Ukuphazanyiswa kwe-Algae: 100% ye-cell disruption ngemva kwe-120 min. sonication. © I-Algae Grow Lab

I-Algae Grow Lab ibhekene ne-ultrasonic ukubhujiswa kweyunithi ehlanganisa imishini ye-Hielscher's ultrasonic ye-cell ukuphazanyiswa (Chofoza ukuze ukhulise!)

Ishadi eligeleza le-Algae Ukukhulisa i-algae yokulima ukulima nokusebenza kweyunithi. © I-Algae Grow Lab

Xhumana Nathi / Cela Ukwaziswa Olwengeziwe

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