Imọ-ẹrọ olutirasandi Hielscher

Ultraacation Deacetylation ti Chitin si Chitosan

Chitosan jẹ biopolymer biotin ti o ni ọpọlọpọ awọn ohun elo ni pharma, ounjẹ, ogbin ati ile-iṣẹ. Deacetylation Ultrasonic ti chitin si chitosan mu itọju pọ ni pataki – yori si ilana ṣiṣe ti o munadoko ati iyara pẹlu idagba chitosan giga ti didara to gaju.

Ultrasonic Chitosan Production

Ti gba Chitosan nipasẹ N-deacetylation ti chitin. Ninu aiṣedeede ti aṣa, chitin ti wa ni apọju ninu awọn ohun elo alumini olomi (paapaa 40 si 50% (w / w) NaOH). Ilana rirẹ beere awọn iwọn otutu to gaju ti 100 si 120ºC jẹ akoko ti o gba akoko pupọ, lakoko ti ikore ti chitosan ti a gba fun igbesẹ ipanu jẹ kekere. Ohun elo ti ultrasonics ti o ni agbara giga mu ilana ilana aiṣedeede ti chitin ṣe pataki ati awọn abajade ninu eso giga ti chitosan iwuwo-kekere iparun ni itọju iyara ni iwọn otutu kekere. Awọn abajade isakalẹ Ultrasonic ni chitosan didara-didara eyiti o lo bi ounjẹ ati eroja pharma, bi ajile ati ninu ọpọlọpọ awọn ohun elo ile-iṣẹ miiran.
Awọn abajade itọju Ultrasonic ni iwọn iyasọtọ ti acetylation (DA) ti chitin ṣe iwọn alefa ti acetylation chitin lati DA≥90 si chitosan pẹlu DA≤10.
Ọpọlọpọ awọn ijinlẹ iwadii jẹrisi ndin ti maini chitin ultrasonic si chitosan. Weiss J. et al. (2008) rii pe sonication ṣe iyipada iyipada ti chitin si chitosan drastically. Itọju ultrasonic ti chitin wa pẹlu akoko ifowopamọ pataki ti dinku akoko ilana ti a beere lati awọn wakati 12 si wakati diẹ si. Pẹlupẹlu, a nilo epo diẹ lati ṣe aṣeyọri iyipada ni kikun, eyiti o dinku ipa ayika ti nini lati tuka ati sọ nkan ti o lo tabi epo ele ti a ko pinnu, ie NaOH ogidi.

Ultraacation Deacetylation ti Chitin si Chitosan

Deacetylation ti chitin si chitosan ni igbega nipasẹ sonication

Ultrasonicator UIP4000hdT giga-giga fun awọn ohun elo ile-iṣẹ

UIP4000hdT – 4kW agbara ultrasonic eto

Ibere ​​Alaye




Akiyesi wa Ìpamọ Afihan.


Ṣiṣẹ Ilana ti itọju Ultrasonic Chitosan

Agbara giga, ultrasonication kekere-igbohunsafẹfẹ (∼20-26kHz) ṣẹda cavitation acoustic ninu awọn olomi ati awọn slurries. Olutirasandi agbara giga ṣe igbelaruge iyipada ti chitin si chitosan bi epo (fun apẹẹrẹ, NaOH) awọn ẹya ara ati ki o wọ awọn patikulu chitin to lagbara, nitorinaa n sọ agbegbe agbegbe pọ si ati imudara gbigbe gbigbe laarin agbedemeji ati omi olomi. Pẹlupẹlu, awọn ipa rirẹ-kuru giga ti cavitation ultrasonic ṣẹda awọn ipilẹṣẹ ọfẹ eyiti o mu ifilọlẹ ti reagent (ie NaOH) lakoko hydrolysis. Gẹgẹbi ilana ilana ti kii ṣe igbona, sonication ṣe idibajẹ ibajẹ ile ti n ṣelọpọ chitosan didara to gaju. Awọn akoko processing Ultrasonic kikuru lati jade chitin lati awọn crustaceans bakanna bi eso chitin (ati nitorinaa atẹle chitosan) ti mimọ ti o ga ni akawe si awọn ipo processing ibile. Fun iṣelọpọ ti chitin ati chitosan, ultrasounds nitorina ni agbara lati dinku iye iṣelọpọ, dinku akoko sisẹ, gba fun iṣakoso to dara julọ ti iṣelọpọ ati dinku ipa ayika ti egbin ilana.

Awọn anfani ti Ultra Production Chitosan

  • Giga Chitosan ti o ga julọ
  • Didara didara
  • Akoko ti o dinku
  • Iwọn otutu Ilana
  • Alekun Imudara
  • Rọrun & Isẹ ailewu
  • ayika-ore

Iṣiro-ọrọ Ultraitin Chitin si Chitosan – Ilana

1) Mura awọn chitin:
Lilo awọn ota ibon nlanla bi ohun elo orisun, awọn ota ibon inu yẹ ki o wẹ daradara ni ibere lati yọ eyikeyi oganisimu ati awọn isọdi ti ko ni ibamu pẹlu ile ati amuaradagba. Lẹhinna, ohun elo ikarahun gbọdọ wa ni gbigbẹ patapata (fun apẹẹrẹ, ni 60ºC fun 24h ni adiro). Awọn ota ibon ti o gbẹ jẹ lẹhinna ilẹ (fun apẹẹrẹ lilo ọlọ ha), ṣi silẹ ni alabọde ipilẹ (fun apẹẹrẹ, NaOH ni apejọ kan ti 0.125 si 5.0 M), ati demineralized ninu acid (fun apẹẹrẹ, dilute hydrochloric acid).
2) Deacetylation Ultrasonic
Lati ṣiṣẹ idaṣẹ aṣoju pipadanu ultrasonic aṣoju, awọn patikulu-chitin (0.125 mm) < d < 0.250 mm) are suspended in 40% (w/w) aqueous NaOH at a ratio beta-chitin/NaOH aqueous solution of 1/10(g mL-1), idadoro naa ti gbe lọ si onigun gilasi ti onirin ni ilopo-meji ati pe o jẹ ati sonicated nipasẹ lilo Hielscher kan UP400St ultrasonic homogenizer. Awọn awọn atẹle wọnyi (cf. Fiamingo et al. 2016) ni a tọju igbagbogbo nigbati a ba n mu ifẹhinti itọsi chitin ultrasonic: (i) iwadi ultrasonic (sonotrode Hielscher S24d22D, iwọn ila opin = 22 mm); (ii) Ipo iṣọn-ẹjẹ sonication (IP = 0.5sec); (iii) ultrasonic dada kikankikan
(Mo = 52,6 W cm-2), (iv) iwọn otutu esi (60ºC ± 1ºC), (v) akoko ifura (50 min), (vi) ipin beta-chitin iwuwo / iwọn didun ti 40% (w / w) iṣuu soda sodaxide (BCHt / NaOH = 1) / 10 g mil-1); (vii) iwọn didun idaduro beta-chitin (50mL).
Idahun akọkọ n tẹsiwaju fun 50min labẹ idalẹku oofa nigbagbogbo ati pe lẹhinna ni idilọwọ nipasẹ didi idaduro ni kiakia si 0ºC. Lẹhinna a ṣe afikun iyọ hydrochloric acid lati ni pH 8.5 ati pe ayẹwo CHs1 ti wa ni sọtọ nipasẹ sisẹ, wẹ pẹlu omi pupọ ati fifọ ni awọn ipo ibaramu. Nigbati a ba tun mu esekuro ultrasonic kanna bi igbesẹ keji si CHs1, o ṣe agbejade CHs2 ayẹwo.

Ultraacacacacation ti chition si chitosan

Ṣiṣayẹwo awọn aworan microscopy elektronia (SEM) ni titobi ti 100 × ti a) gladius, b) gladius olutirasandi, c) ch-chitin, d) olutọju olutirasandi β-chitin, ati e) chitosan (orisun: Preto et al . 2017)

Fiamingo et al. ri pe aiṣedeede ultrasonic ti beta-chitin daradara mu chitosan iwuwo molikula giga giga pẹlu iwọn kekere ti acetylation boya lilo awọn afikun tabi bugbamu inert tabi awọn akoko ifesi gigun. Paapaa biotilẹjẹpe ifasilẹ itọsi ultrasonic ti gbe jade labẹ awọn ipo milder – ie iwọn otutu lenu nigba ti a ba ṣe afiwe si awọn deacetylations thermochemical julọ. Deacetylation ultrasonic ti beta-chitin ngbanilaaye igbaradi ti chitosan jijẹ laileto ti o gba alefa iwọn ti acetylation (4% ≤ DA ≤ 37%), iwuwo iwuwo molikula giga giga (900,000 g mol)-1 MW 200 1,200,000 g mol-1 ) ati isọkusọ kekere (1.3 ≤ Ð ≤ 1.4) nipasẹ gbigbe awọn ifa mẹta ni itẹlera (50 min / ni igbese) ni 60ºC.

Hielscher Ultrasonics ṣe awọn iṣẹ ultrasonic-giga ultrasonic fun awọn ohun elo sonochemical.

Awọn oniṣẹ ultrasonic agbara giga lati laabu lọ si awọn ọkọ ofurufu ati iṣẹ-ṣiṣe iṣẹ.

Awọn ohun elo Ultrasonic Igbara-giga fun Gbigbe Chitosan

UIP4000hdT - 4 kilowatts eto eto agbara ultrasonic fun isediwon ati ibajẹ ti epo olifi wundia ni afikunPipin pipin ti chitin ati aṣẹ ti chitin si chitosan nilo awọn ohun elo ultrasonic ti o lagbara ati ti o gbẹkẹle ti o le fi ọgbọn giga ga, nfunni iṣakoso pipe ni pato lori awọn ilana ilana ati pe o le ṣiṣẹ 24/7 labẹ ẹru wuwo ati ni awọn agbegbe eletan. Hielscher Ultrasonics ọja ibiti o gba iwọ ati awọn ibeere ilana rẹ bo. Awọn olutirasandi Hielscher jẹ awọn iṣẹ ṣiṣe giga ti o le ni ipese pẹlu awọn ẹya ẹrọ bi sonotrodes, awọn olupolowo, awọn oludari tabi awọn sẹẹli ṣiṣan lati baamu awọn ibeere ilana rẹ ni ọna ti aipe.
Pẹlu ifihan awọ awọ oni-nọmba, aṣayan lati tito sonication ṣiṣe, gbigbasilẹ data laifọwọyi lori kaadi SD ti a ti ṣakopọ, iṣakoso aṣàwákiri latọna jijin ati ọpọlọpọ awọn ẹya diẹ sii, iṣakoso ilana ilana giga ati ore-olumulo jẹ iṣeduro. Ni asopọ pẹlu jija ati agbara ẹru fifuye wuwo, awọn ọna ṣiṣe ultrasonic Hielscher jẹ ẹṣin iṣẹ igbẹkẹle rẹ ni iṣelọpọ.
Pipin Chitin ati pipinjẹ nilo olutirasandi lagbara lati gba iyipada ti a pinnu ati ọja chitosan igbẹhin ti didara giga. Paapa fun pipin ti awọn flakes chitin, awọn titobi giga ati awọn titẹ giga jẹ pataki. Hielscher Ultrasonics’ awọn ilana ultrasonic ile-iṣẹ ni irọrun fi awọn ọgbọn giga gaan. Awọn amplitudes ti o to 200µm le ṣee ṣiṣẹ ni igbagbogbo ni iṣẹ 24/7. Fun paapaa awọn titobi giga, awọn sonotrodes ultrasonic ti adani wa o si wa. Agbara agbara ti awọn ọna ṣiṣe ultrasonic Hielscher gba laaye fun pipaṣẹ deacetylation daradara ati iyara ni ilana ailewu ati olumulo ore.

Awọn tabili ni isalẹ yoo fun ọ ni itọkasi ti agbara gbigbe agbara ti wa ultrasonicators:

ipele iwọn didun Oṣuwọn Tisan Niyanju awọn ẹrọ
1 si 500mL 10 si 200mL / min UP100H
10 si 2000mL 20 si 400mL / min UP200Ht, UP400St
0.1 si 20L 0.2 si 4L / min UIP2000hdT
10 si 100L 2 si 10L / min UIP4000hdT
na 10 si 100L / min UIP16000
na tobi oloro ti UIP16000

Pe wa! / Beere Wa!

Bere fun alaye sii

Jowo lo awọn fọọmu isalẹ, ti o ba fẹ lati beere afikun alaye nipa homogenization ultrasonic. A yoo dun lati fun ọ ni eto ultrasonic kan ti n ṣe awọn ibeere rẹ.









Jọwọ ṣe akiyesi wa Ìpamọ Afihan.


Iwe-iwe / Awọn itọkasi

  • Butnaru E., Stoleru E., Brebu MA, Darie-Nita RN, Bargan A., Vasile C. (2019): Awọn Filimimu Chitosan-based Bionanocomposite Ṣetan Ni Imọlẹ Emulsion fun Ilana Ounje. Awọn ohun elo 2019, 12 (3), 373.
  • Fiamingo A., de Moura Delezuk JA, Trombotto St. David L., Campana-Filho SP (2016): Pupọ fẹẹrẹ ti iwuwo molikula ti chitosan lati ipilẹṣẹ olutọju olutirasandi multistep ti beta-chitin. Ultrasonics Sonochemistry 32, 2016. 79-85.
  • Kjartansson, G., Wu, T., Zivanovic, S., Weiss, J. (2008): Iyipada-iranlọwọ Sonochemically ti Chitin si Chitosan, Ipade Awọn oludari Awọn Iwadi Iṣeduro ti USDA, New Orleans, LA, June 28th.
  • Kjartansson, G., Kristbergsson, K. Zivanovic, S., Weiss, J. (2008): Ipa ti otutu nigba deacetylation ti chitin si chitosan pẹlu olutirasandi giga bi itọju-iṣaaju, Ipade Ọdọọdun ti Institute of Technologists Food , Ilu Ilu Tuntun, LA, Oṣu Kini Ọjọ 30th, 95-18.
  • Kjartansson, G., Kristbergsson, K., Zivanovic, S., Weiss, J. (2008): Ipa ti olutirasandi giga lati mu yara iyipada ch china si chitosan, Ipade Ọdọọdun ti Institute of Technologists Food, Orleans New, LA, Oṣu Kini Ọjọ 30th, 95-17.
  • Preto MF, Campana-Filho SP, Fiamingo A., Cosentino IC, Tessari-Zampieri MC, Abessa DMS, Romero AF, Bordon IC (2017): Gladius ati awọn itọsẹ rẹ gẹgẹbi agbara biosorbents ti o pọju fun epo epo epo. Ijinlẹ ti Ayika ati Iwadi Ipoti Ayika (2017) 24: 22932-2239.
  • Wijesena RN, Tissera N., Kannangara YY, Lin Y., Amaratunga GAJ, de Silva KMN (2015): Ọna kan fun igbaradi oke ti awọn ẹwẹ titobi chitosan ati awọn nanofibers. Awọn Polymers Carbohydrate 117, 2015. 731-77.
  • Wu, T., Zivanovic, S., Hayes, DG, Weiss, J. (2008). Iwọn ti o munadoko ti iwuwo molikula chitosan nipasẹ olutirasandi giga-giga: Ẹrọ ti o ni labẹ ati ipa ti awọn aye iṣelọpọ. Iwe akosile ti Iṣẹ-ogbin ati Kemistri Ounjẹ 56 (13): 5112-5119.
  • Yadav M .; Goswami P .; Paritosh K .; Kumar M .; Pareek N.; Vivekanand V. (2019): Egbin Ẹja Omi: orisun kan fun igbaradi ti awọn ohun elo chita / chitosan ti iṣowo oojọ ti iṣowo. Awọn orisun bioresosi ati Bioprocessing 6/8, 2019.


Awọn Otitọ Tita Mọ

Bawo ni Ultraact Chitin Deactylation Ṣiṣẹ?

Nigbati agbara giga, olutirasandi-kekere igbohunsafẹfẹ (fun apẹẹrẹ, 20-26kHz) ni a ṣe sinu omi tabi ṣiṣan, maili awọn igigirisẹ giga-kekere / kekere awọn kẹkẹ kekere ni a lo si omi ṣiṣẹda ifunmọ ati rare. Lakoko awọn igbọnwọ giga agbara-kekere / awọn kẹkẹ kekere-kekere, awọn eefa atẹgun kekere ti wa ni ipilẹṣẹ, eyiti o dagba lori ọpọlọpọ awọn kẹkẹ titẹ. Ni aaye, nigbati awọn eefa idoti ko le gba agbara diẹ sii, wọn ṣubu lulẹ. Lakoko implosion eefin yii, awọn ipo kikankikan ni agbegbe waye: awọn iwọn otutu to ga si 5000K, awọn titẹ ti o to 2000atm, awọn oṣuwọn alapapọ / itutu agbawo ga pupọ ati awọn iyatọ iyatọ waye. Niwọn igba iṣegun iṣuu iṣu yiyara ti o pọ ju ibi gbigbe lọ ati gbigbe ooru, agbara inu iho iparun ti wa ni ala si agbegbe kekere kan, tun pe ni “aaye ti o gbona”. Lodi ti oyun cavitation tun nyorisi microturbulences, awọn ọkọ oju omi omi ti o to 280m / s velocity ati awọn ipa rirẹ-kuru. Iwa yii ni a mọ bi ultrasonic tabi cavitation acoustic.
Awọn eegun ati awọn patikulu ninu omi ti a fi sinu ọmọ ti ni idiwọ nipasẹ awọn agbara cavitational ati nigbati awọn patikulu isare ti o pọ si ara wọn, wọn fa lilu nipasẹ ikọlu aarin. Cavitation Acoustic ni ipilẹ ṣiṣẹ ti milling ultrasonic, tuka, emulsification ati sonochemistry.
Fun deacetylation chitin, olutirasandi olutirasandi giga ni agbegbe dada nipa ṣiṣan dada ati igbega gbigbe gbigbe pupọ laarin awọn patikulu ati reagent.

chitosan

Chitosan jẹ papọ, cationic, polymer carbohydrate carbohydrate ti kii ṣe majele ti pẹlu eka ti iṣakoso kemikali ti a ṣe nipasẹ awọn ẹya glucosamine gẹgẹbi as akọkọ akọkọ (> 80%) ati awọn sipo N-acetyl glucosamine (<20%), randomly distributed along the chain. Chitosan is derived from chitin through chemical or enzymatic deacetylation. The degree of deacetylation (DA) determines the content of free amino groups in the structure and is used to distinguish between chitin and chitosan. Chitosan shows good solubility in moderate solvents such as diluted acetic acid and offers several free amine groups as active sites. This makes chitosan advantageous over chitin in many chemical reactions. Chitosan is valued for its excellent biocompatibility and biodegradability, non-toxicity, good antimicrobial activity (against bacteria and fungi), oxygen impermeability and film forming properties. In contrast to chitin, chitosan has the advantage of being water-soluble and thereby easier to handle and use in formulations. As the second most abundant polysaccharide following cellulose, the huge abundance of chitin makes it a cheap and sustainable raw material.

Chitosan Production

Chitosan ni iṣelọpọ ni ilana igbesẹ meji. Ni igbesẹ akọkọ, ohun elo ti aise, gẹgẹ bi awọn ikẹkun crustacean (ie shrimp, akan, lobster), ti wa ni isokuro, demineralized ati mimọ lati gba chitin. Ni igbesẹ keji, a tọju chitin pẹlu ipilẹ to lagbara (fun apẹẹrẹ, NaOH) lati yọ awọn ẹwọn ẹgbẹ acetyl lati le gba chitosan. Ilana ti iṣelọpọ chitosan ti mora ni a mọ lati jẹ akoko pupọ ati iye owo to lekoko.

chitin

Chitin (C8H13O5N)n jẹ polima taara-ti-pq ti β-1,4-N-acetylglucosamine ati pe o jẹ ipin sinu α-, β- ati γ-chitin. Jije itọsi ti glukosi, chitin jẹ paati akọkọ ti awọn exoskeletons ti arthropods, bii crustaceans ati awọn kokoro, radulae ti molluscs, awọn beakslopod, ati awọn irẹjẹ ẹja ati awọn lissamphibians ati pe a le rii ni awọn sẹẹli alagbeka ni elu, ju. Iwọn ti chitin jẹ afiwera si cellulose, dida awọn nanofibrils kirisita tabi awọn ajile. Cellulose jẹ polysaccharide ti o lọpọlọpọ julọ ti agbaye, atẹle nipa chitin bi keji julọ lọpọlọpọ polysaccharide.

glucosamine

Glucosamine (C6H13KO5) jẹ suga amino ati iṣaju pataki pataki ninu iṣelọpọ biokemika ti awọn ọlọjẹ glycosylated ati awọn ẹfọ. Nipa ti ara jẹ apọju lọpọlọpọ ti o jẹ apakan ti iṣeto ti awọn polysaccharides mejeeji, chitosan, ati chitin, eyiti o jẹ ki glucosamine jẹ ọkan ninu monosaccharides pupọ julọ. Pupọ ti glucosamine ti iṣowo ti o wa ni iṣelọpọ nipasẹ iṣuu hydrolysis ti awọn exoskeletons crustacean, ie ie akan ati awọn ikẹbu nla.
A nlo Glucosamine ni pataki bi afikun ti ijẹun nibiti o ti lo ni awọn ọna ti imi-ọjọ glucosamine, glucosamine hydrochloride tabi N-acetyl glucosamine. Awọn afikun imi-ọjọ glucosamine ni a ṣakoso nipasẹ ẹnu lati tọju ipo ti o fa irora ti o fa nipasẹ iredodo, fifọ ati ipadanu iṣẹlẹ ti iṣọn-ẹjẹ (osteoarthritis).