Imọ-ẹrọ olutirasandi Hielscher

Bawo ni Lati Lo Hielscher's Ultrasonic Tissue Homogenizers

Ṣaaju ṣiṣe iwadii tabi isọtọ ti awọn macromolecules intracellular gẹgẹbi awọn ọlọjẹ, awọn organelles, awọn enzymu tabi awọn agbo-iṣẹ ti nṣiṣe lọwọ, ọna ṣiṣe ti o dara fun lysis tissu ati ipasasẹ alagbeka. Ultrasonication jẹ ọna ti o wulo fun igbaradi ti ara ẹni ti o ṣawari awọn ohun elo intracellular lati inu apo-inu inu inu foonu ni kiakia sinu imuduro idutu. Awọn igbẹkẹle ologun ti ultrasonic fabrics homogenization ni a le tunṣe lẹsẹsẹ si awọn ohun elo kan pato, boya igbasilẹ asọ ti o lagbara (fun apẹẹrẹ fun DNA / RNA) nipasẹ sonication laipẹ tabi idaruku cell cellujẹ (fun apẹẹrẹ fun nannochloropsis, iwukara) nipasẹ ultrasonic cavitation.

Wa ni isalẹ asayan ti awọn tissues, awọn sẹẹli ati awọn ohun elo miiran ti o ni imọran pẹlu awọn ilana sonication ati awọn iṣeduro ti o ni ibatan, bi o ṣe le ṣetan ayẹwo rẹ daradara nipa lilo homogenizer ultrasonic kan.

Ultrasonication jẹ ọna ti o dara julọ fun igbaradi ayẹwo pẹlu awọn ohun elo ti cell lysis, idalọwọduro, iparun & isediwon.

Ultrasonic tissu homogenizer UP100H

Bawo ni lati Ṣetan Lysates rẹ, Homogenates ati Awọn Afikun lati Awọn Ohun elo Ti Ẹmi

Ni itọsọna alphabetical:

Acetobacter suboxydans

Ohun elo Ultrasonic:
Ẹyọ iṣelọpọ ni iṣẹju 5-15.
Iṣeduro ẹrọ:
UP200St

Actinomyces

Ohun elo Ultrasonic:
Idalọwọduro ati amuaradagba amuaradagba ni 3-5 min.
Iṣeduro ẹrọ:
UP50H

ALP ati LDH iṣẹ

Ohun elo Ultrasonic:
Ipinnu ti ALP ati LDH iṣẹ ati akoonu amuaradagba
Awọn ayẹwo ẹyin ti a tio tutunini ni a gbẹ fun 20 min lori yinyin, tẹle pẹlu lysis cell pẹlu PBS ti o ni 1% Triton X-100 fun 50 min lori yinyin. Nigba cell lysis kọọkan ayẹwo ti sonicated fun 1 min ni 80 W pẹlu ohun profaili ultrasonic UP100H (Hielscher Ultrasonics).
Iṣeduro ẹrọ:
UP100H
Itọkasi / Iwe Iwadi:
Bernhardt, A. et al. (2008): Ẹka nkan ti iṣelọpọ-ẹya-ara ti o wa ni artificial, egungun-ara miiran ti o wa ninu awọ-ṣe iṣeduro iyatọ osteogenic ti awọn sẹẹli stromal egungun.

Amorphous tricalcium phosphate (ATCP)

Ohun elo Ultrasonic:
ATCP awọn ohun-elo-ti-ni-ni-ATCP, eyiti o jẹ akọkọ ti o ṣe pataki fun iṣeto ti hydroxyapatite, ni a ti tuka ni chloroform ti o ni 5% (w / w) Tween20 tọka PLGA nipa lilo ẹrọ Hielscher ultrasonic UP400S ni 320W fun 5 min. lilo awọn aaye arin pulsed (50%) lati gba fun isinmi ti awọn patikulu.
Iṣeduro ẹrọ:
UP400S
Itọkasi / Iwe Iwadi:
Mohn, Dirk; Ege, Duygu; Feldman, Kirifl; Schneider, Oliver D .; Imfeld, Thomas; Boccaccini, Aldo R. (2014): Oro kalisiomu ti fosifeti nanoparticle fillers gba itọju polymer ti awọn ẹrọ imudani egungun pẹlu agbara bioactivity. Awọn ọfẹ Library 01 May 2010. 21 January 2014.

Anthocyanins

Ohun elo Ultrasonic:
Anthocyanins: di-glucosides, mono-glucoside, monoglucosides acylated ati awọn di-glucosides acylated ti peonidin, malvidin, cyanidin, petunidin ati delphinidin: isediwon lati inu eso ajara ni 40 iṣẹju sẹhin; pH 5.0; ipin ti awọn ohun elo / isediwon nkan ti 1: 6.
Iṣeduro ẹrọ:
UP100H

Anthraquinones

Ohun elo Ultrasonic:
Isediwon ti Anthraquinones lati awọn orisun Morinda citrifolia
Iṣeduro ẹrọ:
UP400S

Apricot awọn irugbin

Ohun elo Ultrasonic:
Imuju-iṣaaju ti ultrasonic ṣaaju ki o to yọ epo lati awọn irugbin Jatropha curcas L. lati mu isediwon sii.
Iṣeduro ẹrọ:
UP400S

Arcisia sèkílọ Turcz

Ohun elo Ultrasonic:
Isediwon ti Rutin (1.0g milled sample in 30 mL methanol) ni 25 ° C ni 5-10 min.
Iṣeduro ẹrọ:
UP50H

Afun aspergillus

Ohun elo Ultrasonic:
Ultrasonic inactivation of Aspergillus flavus in Sabouraud growth medium
Iṣeduro ẹrọ:
UP200S

B. sphaericus / Bacillus sphaericus

Ohun elo Ultrasonic:
Idalọwọduro ni 1-3 min.
Iṣeduro ẹrọ:
UP200S

Bacillus anthracis sterne 34F2 spores

Ohun elo Ultrasonic:
Iyọkuro ultrasonic ti Bacillus anthracis sterne 34F2, Bacillus cereus ATCC 21281, ati Bacillus thuringiensis ATCC 33680 spores. Awọn lilo ti 150 ppm ti sodium hypochlorite pẹlú pẹlu olutirasandi ṣe ja si spore inactivation.
Iṣeduro ẹrọ:
UP200S ni iwọn 100%
Itọkasi / Iwe Iwadi:
Pamarthi, SR et al .: Imudarasi ti olutirasandi ni ibi ipalọlọ Bacillus spp spores Ti a fi sinu Awọn Ẹrọ Awọn Ounje Awọn Ẹka ti a ti ṣopọ si Awọn Ibaraẹnisọrọ Olupọ.

Bacillus cereus ATCC 21281 spores

Ohun elo Ultrasonic:
Iyọkuro ultrasonic ti Bacillus anthracis sterne 34F2, Bacillus cereus ATCC 21281, ati Bacillus thuringiensis ATCC 33680 spores. Awọn lilo ti 150 ppm ti sodium hypochlorite pẹlú pẹlu olutirasandi ṣe ja si spore inactivation.
Iṣeduro ẹrọ:
UP200S ni iwọn 100%
Itọkasi / Iwe Iwadi:
Pamarthi, SR et al .: Imudarasi ti olutirasandi ni ibi ipalọlọ Bacillus spp spores Ti a fi sinu Awọn Ẹrọ Awọn Ounje Awọn Ẹka ti a ti ṣopọ si Awọn Ibaraẹnisọrọ Olupọ.

Bacillus subtilis

Ohun elo Ultrasonic:
Agbara giga, igbohunsafẹfẹ alailowaya kekere ni awọn ipele kekere ti awọn abajade idaduro awọn kokoro aisan ni idinku awọn idiwọn ninu awọn nọmba cellular bacterial ie ti o pọju pa. Ni awọn ipele ti o tobi, awọn esi sonication ni ibẹrẹ akọkọ ninu awọn nọmba cellu ti o n ṣe ipinnu idinku awọn kokoro arun ṣugbọn gbigbọn akọkọ yii lẹhinna ṣubu bi idibajẹ ti pari ati pe oṣuwọn pa a ṣe pataki.
Iṣeduro ẹrọ:
UP200St
Itọkasi / Iwe Iwadi:
Joyce, E .; Pull, SS; Lorimer, JP; Mason, TJ (2003): Awọn idagbasoke ati imọran ti olutirasandi fun itoju ti awọn kokoro suspensions. Iwadi ti igbohunsafẹfẹ, agbara ati akoko sonication lori awọn eya Bacillus. Ultrason. Sonochem. 10/2003. pp. 315-318.

Barley's Alpha-amylase

Ohun elo Ultrasonic:
Ṣiṣepo tabi didiṣe iṣẹ-ṣiṣe ti Alpha-amylase Barley: Awọn ti o ṣafihan (10 g awọn irugbin barge) ti wa ni tuka ni 80 milimita ti omi tẹ ni taara sonication ni ultrasonic kikankikan ti 20, 60 ati 100% titobi, cyle ti 50% ati pẹlu afikun agitation . Awọn sonotrode ti a immersed nipa 9 mm sinu ojutu. O ti ṣe atunse ojutu ni otutu otutu ti 30C ° fun 5, 10 ati 15 min.
Iṣeduro ẹrọ:
UP200S, amplitudes: 20, 60 ati 100%; iwo ti 50%; Sonotrode S3, 30C °.
Itọkasi / Iwe Iwadi:
Yaldagard et al. (2008): Ipa ti Power Ultrasonic lori aṣayan iṣẹ-ṣiṣe ti Alpha-amylase Barley lati Igbẹju Ọgba ti Irugbin

Awọn alabirin Barnacle

Ohun elo Ultrasonic:
Iyọkuro iṣelọpọ / pipa ti mesozooplankton: nipasẹ sonication labẹ awọn ipo wọnyi ti awọn oṣuwọn sisan mẹrin (200, 400, 520 ati 800 LH-1) ati awọn titobi mẹrin (25, 50, 75 ati 100%) iwọn pa laarin 61 ati 97% ti waye.
Iṣeduro ẹrọ:
UIP2000hd
Itọkasi / Iwe Iwadi:
Viitaslo et al. (2005): Ozone, Ultraviolet Light, Olutirasandi ati omi epo Bi awọn Ballast Omi itọju – Awọn idanwo pẹlu Mesozooplankton Ni Ẹwa Alaini- Omi Ipaju.

Bọsibabacteria strains: B. ṣẹda ATCC 15700, B. animalis subsp. Lactis (BB-12), B. longum (BB-46)

Ohun elo Ultrasonic:
Kokoro iparun cellujẹ ti kokoro ati fifọ ti ß-galactosidase lati awọn iṣọn Bifidobacteria: B. bii ATCC 15700, B. animalis subsp. Lactis (BB-12), B. longum (BB-46): 100 mL wara ti a ti kojọpọ pẹlu aṣeyọri ti a ti lo pẹlu olutirasandi. Cavitation fa idibajẹ awọn ẹyin ti ko ni kokoro ati ni akoko kanna, igbasilẹ ti ß-galactosidase.
Iṣeduro ẹrọ:
UIP1000hd: titobi: 10%, agbara: 80W, titobi: 100%, agbara: 200W; Sonotrode BS2d34; 15-30 min.
Itọkasi / Iwe Iwadi:
Fọọ ati al. (2009): Olutirasandi ṣe iranlọwọ fun Fermentation Wara pẹlu Awọn isẹ.

BL21 (DE3) awọn sẹẹli PAtHNL (awọn ara yhalini ti Arabidopsis)

Ohun elo Ultrasonic:
Ẹyọ idinku: 15 g BL21- (DE3) awọn sẹẹli _pAtHNL ni a daa duro ni pẹlẹpẹlẹ ni saa-fọọmu phosphate fun 50 mM (pH 7.5) ni 0 degC.
Iṣeduro ẹrọ:
UP200S + sonotrode S14D: ni 70W / cm2 (4 x 5 min), tutu ninu iwẹ yinyin kan
Itọkasi / Iwe Iwadi:
Okrob, D. et al (2009): Hydroxynitrile Lyase lati iṣiro Arabidopsis: Agbekale ti Awọn Ifa-ailẹgbẹ fun Iyanju Cantohydrin nipasẹ Imudara Nikan ati Alailẹgbẹ. Adv. Synth. Catal. 2011, 353, 2399 - 2408.

Awọn ẹyin ẹjẹ (pupa ati funfun)

Ohun elo Ultrasonic:
Idalọwọduro ni 3-10 iṣẹju-aaya.
Iṣeduro ẹrọ:
UP100H

Boldine lati Boldo fi (Peumus boldus Molina)

Ohun elo Ultrasonic:
Ohun pataki lọwọlọwọ ni boldo jẹ boldine ((S) -2,9-dihydroxy-1,10-dimethoxiaporphine), eyiti o jẹ lẹhin catechin ((2S, 3R) -2- (3,4-dihydroxy-phenyl) -3 , 4-dihydro-1 (2H) -benzopyran-3,5,7-triol) awọn ẹya akọkọ ti awọn alkaloid ati ida flavonoid ni awọn boldo leaves. Boldine jẹ oluranlowo antioxidant lagbara ti o gba awọn ibajẹ ti o ni iyọdajẹ ti o ni iyọda ti o ni iyọda ti o ni iyọda ati ti o n ṣe gẹgẹbi ohun iṣiro oloro hydroxyl daradara.
Ilana isediwon: Fun ilana ilana isediwon aṣoju, awọn ayẹwo ti awọn leaves boldo ni a fa jade pẹlu 1L ti omi ti a ti distillated ni titẹ agbara oju aye pẹlu lilo ultrasonicator UIP1000hd ni ipo ati ipo igbasilẹ. Akoko ti awọn iyọọda isinmi laarin 10 ati 40 min., Pẹlu ohun ila-oorun Ultrasonic ti 10 si 23 W / cm2, ati ibiti o wa ni iwọn otutu ti 10 si 70 ° C. Awọn esi ti o dara julọ wer waye labẹ awọn ipo wọnyi: ultrasonic kikankikan ti 23 W / cm2 fun min 40. ni otutu ti 36 ° C
Awọn esi: Awọn abajade iwadi ṣe afihan pe sonication agbara-giga ṣe igbaradi tujade akọsilẹ ti awọn ohun elo matrix ti eweko ti Boldo ni awọn ipo to dara julọ ti o ṣe deede si ọna ti o ṣe deede: ikun ti o yẹ ni tu silẹ nipasẹ sonication ni 30 min. nigbati igbasilẹ akoko ti o jẹ 2h.
Chemat (2013) ati awọn alabaṣiṣẹpọ-iṣẹ ti fihan pe ifasilẹ iranlọwọ nipasẹ ultrasonically-assisted ṣe ṣiṣe daradara ti isediwon ọgbin lakoko idinku akoko isokuso ni ilosoke ti awọn afikun (iye kanna ti epo ati ohun elo ọgbin). Onínọmbà fihan pe awọn ipo iṣapeye ni: agbara sonication 23 W / cm2 pẹlu UIP1000hd fun min 40. ati iwọn otutu ti 36 ° C. Awọn ifilelẹ ti a ṣe ayẹwo ti isediwon olutirasandi n pese isediwon ti o dara ju ti iṣọpọ ti o ni ibamu pẹlu akoko iṣakoso (30 min. Dipo 120 min.), Ikun ti o ga, agbara agbara ti o ga, imudara didara, aabo to ga julọ ati didara didara ọja.
Iṣeduro ẹrọ:
UIP1000hd pẹlu sonotrode BS2d34 ati sisan alagbeka
Itọkasi / Iwe Iwadi:
Petigny, L .; Périno-Issartier, S .; Wajsman, J .; Chemat, F. (2013): Batch ati Tẹsiwaju olutirasandi Iranlọwọ Iranlọwọ Afikun ti Boldo Lea (Peumus boldus Mol.). Iwe Iroyin International ti Imọ-ọpọlọ Imọ-ara Oṣuwọn 14, 2013. 5750-5764.

Bovine omi ara albumin (BSA)

Ohun elo Ultrasonic:
Microencapsulation ni poly (lactic-co-glycolic acid) ni 40 iṣẹju-aaya.
Iṣeduro ẹrọ:
dmini
Itọkasi / Iwe Iwadi:
Freitas et al. (2005): Isan-nipasẹ ultrasonic emulsification ni idapo pelu mimiromicing stic fun aseptic gbóògì ti microspheres nipasẹ epo isediwon.

Igi ọpọlọ + adọnuje

Ohun elo Ultrasonic:
Pipinka ati ipamọ nucleotid; Iwọn iwọn ayẹwo: awọn aami iwon miligiramu 10 milimita 10.
Iṣeduro ẹrọ:
UP50H

Candida albicans

Ohun elo Ultrasonic:
Idalọwọduro ti 15mL ni 9min.
Iṣeduro ẹrọ:
UP100H

Awọn carbohydrates, polysaccharides ati awọn agbo-iṣẹ miiran ti iṣẹ

Ohun elo Ultrasonic:
Isediwon ti awọn carbohydrates, awọn polysaccharides ati awọn agbo-iṣẹ miiran ti iṣẹ
Iṣeduro ẹrọ:
UP200St

Carnosic acid lati rosemary

Ohun elo Ultrasonic:
Iyọkuro ti carnosic acid, ẹya ti nṣiṣe lọwọ, lati rosemary.
Iṣeduro ẹrọ:
UP400S

Capsaicinoids

Ohun elo Ultrasonic:
Isediwon ti awọn capsaicinoids (capsaicin, nordihydrocapsaicin) lati awọn ata alali: Capsaicinoids lati Capsicum frutescens A gba awọn ata nipasẹ ultrasonic isediwon labẹ awọn ipo wọnyi: epo: 95% (v / v) ethanol, ipin / ipin-ipilẹ ti 10 milimita / g, 40 min. akoko isunkuro sonication, 45 ° C isediwon otutu. Isoro jade: 85% ti awọn okun
Iṣeduro ẹrọ:
UP400S

Carotenoids, Beta-Carotenoids

Ohun elo Ultrasonic:
Isediwon lati Karooti.
Iṣeduro ẹrọ:
UP50H

cDNA

Ohun elo Ultrasonic:
Poly-A RNA ti wẹ pẹlu awọn ohun elo imudani mRNA ti Dynabeads (Invitrogen) tẹle awọn itọnisọna olupese ati pe a ṣe itọju fun 30 min ni 37 ° C pẹlu TURBO DNase (Ambion 0,2 units / 1 μg ti RNA). Akọkọ- ati ọna asopọ keji-tẹle ilana ilana olupese. Nipa 500ng ti cDNA ti o ni ilọpo meji ni sonication pẹlu Hielscher's UTR200. DNA ti pin ni 2% giga gelu agarose ati awọn ajẹkù 230-270 ti a ge.
Iṣeduro ẹrọ:
UTR200 tabi TD_CupHorn
Itọkasi / Iwe Iwadi:
Delft, J. van; Gaj, St .; Lienhard, M .; Albrecht, MW; Kirpiy, A; Brauers, K .; Claessen, S .; Lizarraga, D .; Lehrach, H .; Herwig, R .; Kleinjans, J. (2012): RNA-Seq n fun awọn imọran titun ni awọn idahun ti Transcriptome Indiced nipasẹ awọn Carcinogen Benzo [a] pyrene. Awọn ẹkọ ẹkọ Toxicological 130/2, 2012. 427-439.

Caryophanon latum

Ohun elo Ultrasonic:
Iyọkuro ti glucosamine, acid muramic, alanine, glutamic acid ati lysine lati akọsilẹ caryophanon.
Iṣeduro ẹrọ:
UP100H

Cellulose

Ohun elo Ultrasonic:
Homogenization / igbaradi ti isotopically homogeneous cellulose.
Iṣeduro ẹrọ:
UP200S; ni yinyin iwẹ
Itọkasi / Iwe Iwadi:
Laumer et al. (2009): Atilẹwe iwe kan fun homogenization ti cellulose lati lo awọn ọja microamounts fun awọn itupalẹ isotope stables.

Awọn nanocrystals Cellulose (CNC) ti a pese lati awọn CNCs cellulose eucalyptus

Ohun elo Ultrasonic:
Awọn nanocrystals Cellulose (CNC) ti a pese lati inu awọn CNCs eucalyptus cellulose ti a ṣe atunṣe nipasẹ iṣesi pẹlu methyl adipoyl chloride, CNCm, tabi pẹlu adalu acetic ati sulfuric acid, CNCa. Nitorina, awọn CNCs ti a ti di gbigbọn, CNCm ati CNCa ti wa ni redispersed ni awọn ohun ti o mọ (EA, THF tabi DMF) ni 0.1 wt%, iṣoro ni iṣelọpọ lokan ni (24 ± 1) C, tẹle 20 min ni yara sonication nipa lilo UP100H Hielscher Ultrasonics (Germany), ni ipese pẹlu sonotrode 130 W / cm2, ni 24 ± 1 degC. Lẹhin eyi, a fi CAB si afikun pipọ CNC, ki o jẹ pe polymer concentration ni 0.9 wt%.
Iṣeduro ẹrọ:
UP100H; ni 24 degC fun 20 min.
Itọkasi / Iwe Iwadi:
Blachechen, LS et al (2013): Sisọ ibaramu ti iduroṣinṣin colloidal ti awọn nanocrystals cellulose ati pipinka wọn ni matrix cellulose acetate butyrate. Cellulose 2013.

Sellulose lati apo suga

Ohun elo Ultrasonic:
Awọn isediwon ti cellulose lati igba kekere bagasse
Iṣeduro ẹrọ:
UP200St

ChIP assay

Ohun elo Ultrasonic:
A lo Ultrasonication fun lysis sẹẹli lati tu silẹ chromatin. Iṣapẹẹrẹ (ti fa) sonication ni a lo lati fun ni ida chromatin. Pẹlupẹlu, olutirasandi ṣe ifawọn oṣuwọn ti adehun antibody si awọn afojusun awọn ọlọjẹ ati dinku nitorina akoko immunoprecipitation.
Iṣeduro ẹrọ:
UP100H
Itọkasi / Iwe Iwadi:
Basselet, P. et al. (2008): Iṣapẹrẹ iṣapẹrẹ fun onínọmbà orisun-DNA ti enterohemorrhagic Escherichia coli (EHEC).
Lauri, A. (2005): Iṣalaye iṣan ti idagbasoke petal nipasẹ X-ChIP ati imọ-ẹrọ arabara meji. Ile-iwe Itẹwe ti Cologne 2005.

chromatin

Ohun elo Ultrasonic:
Irun ti chromatin
Iṣeduro ẹrọ:
UP400S; ni 30% titobi ati 0.5 ọmọ; lori yinyin.
Itọkasi / Iwe Iwadi:
Oh et al. (2003): Acetyl-CoA Carboxylase Gene Ṣeto nipasẹ Sterol Regulatory Eran-abuda Protein-1 ni Ẹdọ.

Chromatin isediwon

Ohun elo Ultrasonic:
Awọn lysate ti MEL DS19 awọn sẹẹli ti a sonicated pẹlu 10 iyipo ti 20 s kọọkan ni 70% ti o pọju o wu lilo lilo Hielscher 200W ultrasonic processor UP200H
Iṣeduro ẹrọ:
UP200H; 70% ti o pọju o pọju; ipo iṣakoso: 10 iṣẹju ti 20 iṣẹju-aaya. kọọkan.
Itọkasi / Iwe Iwadi:
Kang, H. Ch. ati al (2010): PIAS1 n ṣe ipinnu ipoidojọ CP2c ati iṣelọpọ ti iṣelọpọ ti nṣiṣe lọwọ ni idaniloju a-globin pato-erythroid. Awọn ohun elo Idaabobo Nucleic. 38/16, 2010. ni 5456-5471.

Iwọn-akọọlẹ

Ohun elo Ultrasonic:
Sonication ti adsorbant ninu epo ti yọ agglomerates laarin iṣẹju-aaya diẹ ati o mura aṣọ kan, iwe ti o rọrun ni irọrun. O wulo fun igbaradi adsorbent (fun apẹẹrẹ siliki jeli) saju iwe-akọọlẹ iwe iwe.
Iṣeduro ẹrọ:
UP400S

Awọn abumọ

Ohun elo Ultrasonic:
Idarujẹ sẹẹli ti mesozooplankton
Iṣeduro ẹrọ:
UP2000hd pẹlu sẹẹli sisan
Itọkasi / Iwe Iwadi:
Viitaslo et al. (2005): Ozone, Ultraviolet Light, Olutirasandi ati omi epo Bi awọn Ballast Omi itọju – Awọn idanwo pẹlu Mesozooplankton Ni Ẹwa Alaini- Omi Ipaju.

Awọn agbalagba Copepod ati awọn aṣakokoro

Ohun elo Ultrasonic:
Idarujẹ sẹẹli ti mesozooplankton: nipasẹ sonication labẹ awọn ipo atẹle ti awọn oṣuwọn sisan mẹrin (200, 400, 520 ati 800 lh-1) ati awọn ọgbọn mẹrin (25, 50, 75 ati 100%) oṣuwọn pipa kan laarin 87 ati 99% ti waye .
Iṣeduro ẹrọ:
UIP2000hd pẹlu sẹẹli sisan
Itọkasi / Iwe Iwadi:
Viitaslo et al. (2005): Ozone, Ultraviolet Light, Olutirasandi ati omi epo Bi awọn Ballast Omi itọju – Awọn idanwo pẹlu Mesozooplankton Ni Ẹwa Alaini- Omi Ipaju.

Copepod nauplii

Ohun elo Ultrasonic:
Idarujẹ sẹẹli ti mesozooplankton: nipasẹ sonication labẹ awọn ipo atẹle ti awọn oṣuwọn sisan mẹrin (200, 400, 520 ati 800 lh-1) ati awọn ọgbọn mẹrin (25, 50, 75 ati 100%) oṣuwọn pipa kan laarin 87 ati 99% ti waye .
Iṣeduro ẹrọ:
UIP2000hd pẹlu sẹẹli sisan
Itọkasi / Iwe Iwadi:
Viitaslo et al. (2005): Ozone, Ultraviolet Light, Olutirasandi ati omi epo Bi awọn Ballast Omi itọju – Awọn idanwo pẹlu Mesozooplankton Ni Ẹwa Alaini- Omi Ipaju.

Awọn sẹẹli COS7

Ohun elo Ultrasonic:
Agbeyewo ni ifipamọ 400 μl
Iṣeduro ẹrọ:
UP200S; Awọn kẹkẹ 7
Itọkasi / Iwe Iwadi:
Zaim (2005): Itupalẹ von Nesprin-2 Defizienten Mäusen.

Cryptosporidium parvaum

Ohun elo Ultrasonic:
Ultraac inactivation ti Cryptosporidium parvaum (protozoa) ninu omi.
Iṣeduro ẹrọ:
UP400S
Itọkasi / Iwe Iwadi:
Tsukamoto, Emi.; Yim, B.; Stavarache, CE; Furuta, M .; Hashiba, K .; Maeda, Y. (2004): Inactivation ti Saccharomyces cerevisiae nipasẹ ifihan omi ikuna ultrasonic. Ultrason. Ṣémékémù. 11/2004. Oj. 61-6.

Cubosomes

Ohun elo Ultrasonic:
Cubosomes – boya ofofo tabi doped pẹlu awọn ohun ti o wa ninu iṣan – Ti pese sile nipasẹ pipinka iye to yẹ fun monoolein ni ojutu kan ti Pluronic F108 lilo ultrasonicator UP100H. Lati gba awọn aṣọ awọleke Fuluorisenti, a fọn fluorophore ninu monoolein ti yo o nipasẹ isunmọ itẹmọlẹ ṣaaju pipinka ni Pluronic F108. Ilana kanna ni atẹle nigbati a ti tun mu awọn aṣọ awọleke Fuluorisenti pẹlu quercetin.
Ayẹwo: iwọn ayẹwo: 4 milimita – feleto 96.4 wt% ti omi, 3.3 wt% ti monoolein, 0.3 wt% ti Pluronic F108. Oṣuwọn ti fluorophore jẹ 2.5 × 10−3 ati 2.8 × 10−3 wt%. Iye ti quercetin ti a ṣafikun: 6.4 × 10−6 wt%.
Sonication: UP100H, titobi 90%, iyipo polusi 0.9, fun 10 min.
Iṣeduro ẹrọ:
UP100H
Itọkasi / Iwe Iwadi:
Murgia, S .; Bonacchi, S .; Falchi, AM; Lampis, S .; Lippolis, V .; Meli, V.; Monduzzi, M .; Prodi, L.; Schmidt, J .; Talmon, Y.; Caltagirone, C. (2013): Cubosomes Ifi-Ilẹ Oògùn-ti a ri Orisirisi: Awọn ẹwẹ titobi wapọ fun Awọn ohun elo ti Theranostic Pọju. Langmuir 29, 2013. 6673-6679.

Dekkera bruxellensis

Ohun elo Ultrasonic:
Ultrasonic inactivation ti Dekkera bruxellensis ninu omi
Iṣeduro ẹrọ:
UIP1500hd
Itọkasi / Iwe Iwadi:
Borthwick, KAJ; Coakley, WT; McDonnell, MB; Nowotny, H .; Awọn Benes, E.; Grfschl, .M (2005): Idagbasoke ti iwe afọwọkọ itẹwe tuntun fun idalọwọduro sẹẹli. J. Microbio. Meths. 60/2005. P. 207 -216. / Lörincz, A. (2004): Idalọwọpọ cellular Ultrasonic ti iwukara ni awọn ifura orisun omi. Awọn biosys. Eng 89 / 2004. p. 297-308. / Tsukamoto, Mo.; Yim, B.; Stavarache, CE; Furuta, M .; Hashiba, K .; Maeda, Y. (2004): Inactivation ti Saccharomyces cerevisiae nipasẹ ifihan omi ikuna ultrasonic. Ultrason. Ṣémékémù. 11/2004. Oj. 61-6.

Dekkera / Brettanomyces bruxellensis

Ohun elo Ultrasonic:
Inactivation ni 90-120 iṣẹju-aaya.
Iṣeduro ẹrọ:
UIP1500hd
Itọkasi / Iwe Iwadi:
wo loke

DNA

Ohun elo Ultrasonic:
Pipin DNA: 2 iṣẹju. sonication ti 100μL pẹlu UP100H tabi 4 min. sonication ti 100μL pẹlu UTR200.
Iṣeduro ẹrọ:
UP100H, UTR200 tabi VialTweeter
Itọkasi / Iwe Iwadi:
Larguinho M. et al. (2010): Idagbasoke ti ilana-ọna ipilẹ-iyara ultrasonic ti o munadoko fun pipin DNA.

Awọn sẹẹli Drosophila melanogaster S2

Ohun elo Ultrasonic:
Ifaagun ti awọn ọlọjẹ sẹẹli lati awọn sẹẹli Drosophila melanogaster S2: Awọn sẹẹli S2 tutunini (1.56108) ni a lysed ni 1 mL yinyin-tutu homogenization ifipamọ (100 mM Tris-HCl pH 7.5, 1% SDS) ati osi lori yinyin fun 10 min. A ti pari lysis sẹẹli nipasẹ ultrasonication lori yinyin (6615 bursts, polusi 0 s & 75% kikankikan) pẹlu ẹrọ ultrasonic ultrasonic Hielscher UP200S.
Iṣeduro ẹrọ:
UP200S (200W), ipo kikankikan 75%: bursts 6615, polusi 0 s.
Itọkasi / Iwe Iwadi:
Schwientek, T. et al. (2007): Ilana ọlọjẹ tẹẹrẹ si mucin-Iru O-glycoproteome ti awọn sẹẹli Drosophila melanogaster S2. Awọn alabojuto 7, 2007. p. 3264-3277.

E. awọn ohun itọsẹ coli

Ohun elo Ultrasonic:
Idarudapọ sẹẹli ti E. coli awọn itọsẹ: Awọn pellets ti o tutu ti o baamu si iwọn ayẹwo ti Vculture = 4 / OD mL ni a tun fi silẹ ni 580 μL 10 mM potasiomu fosifeti buffer pH 7, 1 mM EDTA. 20 μL ti lysozyme (ifọkansi ti 1 g L-1) ni a ṣafikun ati idaduro awọn sẹẹli ti wa ni abe lori yinyin fun nipa 30 min. Lẹhin awọn sẹẹli ti ni idiwọ nipasẹ ultrasonication lemọlemọfún pẹlu ultrasonicator (UP 200S Ultraschallprozessor, Dokita Hielscher GmbH, Teltow) lori yinyin, ni titobi 50% fun 20 iṣẹju-aaya. Awọn ipara sẹyin ati insoluble awọn sẹẹli ti o ya sọtọ nipasẹ centrifugation ni 13000 rpm fun 20 min. Awọn ọlọjẹ insoluble ni a fo lẹmeji pẹlu 10mM potasiomu fosifeti 10 miM pH 7, 1 mM EDTA ati ti o fipamọ ni –20 ° C.
Iṣeduro ẹrọ:
UP200S pẹlu titobi 50%
Itọkasi / Iwe Iwadi:
HA (2005): Iṣapeye ti iṣelọpọ amuaradagba iṣelọpọ ti nṣiṣe lọwọ, n ṣawari ipa ti awọn ọlọjẹ ooru-mọnamọna kekere ti Escherichia coli, IbpA ati IbpB, lori ni ṣiṣatunṣe awọn ara ifisi.

Echinococcus granulosus Antigen

Ohun elo Ultrasonic:
Ṣiṣayẹwo sẹẹli ati idapọmọra: Iwọn homogenized ti eefun ti amuaradagba ti ogbo E. granulosus, ti a pese sile nipasẹ didi-thawing ninu omi omi nitrogen ati 42◦C, ti jẹ ọmọ ni 110V, 170W fun 3×15 iṣẹju aaya lori yinyin. Lẹhin iyẹn, a ti jẹ ki ayẹwo fun centminug fun 15min ni 10000g. Iwọn idaabobo ọlọjẹ ni a ṣe nipasẹ ọna Bradford ati ti o fipamọ ni -20◦C.
Iṣeduro ẹrọ:
UP200S; 170W, itutu agbaiye lori yinyin; 3 x 15 iṣẹju-aaya.
Itọkasi / Iwe Iwadi:
Tabar et al. (2010): Serodiagnosis ti Sheep Hydatidosis pẹlu Hydatid Fluid, Protoscolex ati Gbogbo Ara ti Echinococcus granulosus Antigen.

Escherchia coli Gr-

Ohun elo Ultrasonic:
Ultrasonic inactivation ti Escherchia coli Gr- ni wara ati awọn oje.
Iṣeduro ẹrọ:
UP100H
Itọkasi / Iwe Iwadi:
Zenker, M .; Heinz, V.; Knorr, D. (2003): Ohun elo ti olutirasandi iranlọwọ processing gbona fun itọju ati idaduro didara ti awọn ounjẹ omi. J Ounjẹ Prot 66/2003. p. 1642–1649.

Escherchia coli Gr- ni iyo

Ohun elo Ultrasonic:
Ultrasonic inactivation ti Escherchia coli Gr-ni iyo.
Iṣeduro ẹrọ:
UP100H
Itọkasi / Iwe Iwadi:
Duckhouse, H .; Mason, TJ; Phull, SS; Lorimer, JP (2004): Ipa ti sonication lori makirobia makirobia nipa lilo hypochlorite. Ultrason. Ṣémékémù. 11 / 2004. p. 173-176.

Escherchia coli Gr- ninu omi

Ohun elo Ultrasonic:
Ultrasonic inactivation ti Escherchia coli Gr - ninu omi.
Iṣeduro ẹrọ:
UP100H
Itọkasi / Iwe Iwadi:
Furuta, M .; Yamaguchi, M .; Tsukamoto, T .; Yim, B.; Stavarache, CE; Hasiba, K .; Maeda, Y. (2004): Inactivation ti Escherichia coli nipasẹ ifihan si itanna ultrasonic. Ultrason. Ṣémékémù. 11/2004. Oju-iwe 57-60.

Glaucoma, ori nafu ara

Ohun elo Ultrasonic:
Pipin RNA ti awọn ori aifọkanbalẹ aifọkanbalẹ (lati awọn oju eku): Olori aifọkanbalẹ aifọkanbalẹ (ONH) ti rọ lori yinyin ti o gbẹ ati ni fipamọ ni 80 ° C ṣaaju isediwon. RNA ti ya sọtọ nipasẹ sonicating awọn ọmu aifọkanbalẹ tutun ninu ifipamọ isediwon kit nipasẹ lilo iwadii MS 0,5 (UP50H) ati lẹhinna, lẹhin awọn itọnisọna ti a pese nipasẹ ohun elo Arcturus, pẹlu itọju DNase lati yọ eyikeyi DNA. RNA ti a mọ di mimọ.
Iṣeduro ẹrọ:
UP50H pẹlu iwadii MS0.5
Itọkasi / Iwe Iwadi:
Johnson et al. (2007): Awọn ayipada agbaye ni Ifaagun Nerve Head Gene Ifaagun lẹhin Ifihan si Titẹ iṣan inu iṣan ninu awoṣe Iwọn Glaucoma kan.

Glycosaminoglycan chondroitin imi-ọjọ (CS)

Ohun elo Ultrasonic:
Pinnu CS nipasẹ Dimethylmethylene Blue Assay
Iyọkuro ti awọn sẹẹli: awọn pelle CS ni awọn iwẹ microcentrifuge ni a tun pada ni milimita 0,5 ti ojutu papain ti 0.1mg / milimita ni iyọ iyọ ti o ni ibamu (HBSS) lilo awọn isọ iṣan ara lati inu iwo olutirasandi (UP 100H, Hielscher Ultrasonics GmbH, Jẹmánì) ni leekan si 1 ati Ikun titobi 100% fun 3 iṣẹju-aaya. Lẹhinna, tito nkan waye ni 60 ° C fun 24 h.
Fun imayosorbent assayosorbent assayosorbent (ELISA), lẹhinna awọn sẹẹli naa ti dawọ duro ninu omi distilled ati omi iparun ni ifọkansi ti awọn sẹẹli 106 / milimita, ati pe wọn wa ni firisa ni -70 ° C ni alẹ ọjọ lati le sọ dẹrọ sẹẹli. Awọn sẹẹli naa lẹhinna ni apọju nipasẹ ultrasonication fun 40 iṣẹju-aaya. ni lilo Higencher ultrasonic àsopọ homogenizer UP100H.
Iṣeduro ẹrọ:
UP100H
Itọkasi / Iwe Iwadi:
Vandrovcova et al. (2011): Ipa ti Awọn akojọpọ ati Chondroitin Sulfate (CS) Awọn Iwọn lori Poly- (Lactide-co-Glycolide) (PLGA) lori MG 63 Osteoblast-Like Awọn sẹẹli. Ẹya ara. Res. 60; 2011. 797-813.

Awọn sẹẹli HaCaT

Ohun elo Ultrasonic:
Awọn atunyẹwo ti awọn sẹẹli HaCaT fun Chromatin Immunoprecipitation (ChIP): Awọn sẹẹli HaCaT ni o wa pẹlu 1% formaldehyde fun iṣẹju 10 ni 37uC. Awọn sẹẹli ti o wa ni irọra ni ifipamọ RIPA ati sonicated lori yinyin pẹlu ẹrọ ultrasonic 100W ni titobi = 1 ati iyipo ojuse = 100% ni iṣẹju 12 ọkan (12 x 1 min.)
Iṣeduro ẹrọ:
UP100H; fun 12 iṣẹju. lori yinyin
Itọkasi / Iwe Iwadi:
Zhang et al. (2007): Basonuclin ṣe agbekalẹ Sub Sub ti Ribosomal RNA Jiini ninu Awọn sẹẹli HaCaT.

Heparin: Depolymerization ti heparin

Ohun elo Ultrasonic:
Ọna ultrasonic n mu ki heparin iwuwo kekere ti molikula (LMWH) ṣiṣẹ. Nitorinaa, heparin faragba hydrogen peroxide-catalyzed radical depolymerization. Ihudapọ naa yarayara o gba to wakati 1, lakoko ti ilana imọ-ẹrọ depolymerization gbarale awọn ipo ihuwasi tutu, ko nilo reagents tabi majele ti ajara, ati pese ọja ti ko ni awọn ohun-ara ti kemikali. Nitorinaa, ilana ultrasonic ti ni ibamu daradara fun iṣelọpọ iwọn nla ti LMWH, ṣugbọn awọn analogs tun ṣe agbejade lati awọn polysaccharides sulfate adayeba.
Ilana Ultrasonic:
Fun awọn depolymerization ti physicochemical ti unfractionated heparin nipasẹ ultrasonic iranwo nipa ipilẹṣẹ depolymerization, a ti tu heparin ninu omi si ifọkansi ikẹhin ti 25 miligiramu / milimita (5 milimita). A ti fi adalu idapọmọra ṣiṣẹ ni lilo ultrasonicator-probe kan UP50H. A ṣe ipese ultrasonicator pẹlu wadi (micro sample MS3) pẹlu iwọn ila opin ti 3mm, eyiti o pese titobi ti 180μm. Olupilẹṣẹ ti ipilẹṣẹ awọn ohun elo igigirisẹ gigun asiko pẹlu igbohunsafẹfẹ 30 kHz ti a ṣe nipasẹ ayọ eletutu. Ti mu idapọ iṣesi naa duro ni 60 ° C ni riakiti Radleys® ati awọn riru omi ultrasonic ni a lo gẹgẹbi ọfin 0.5sec lati yago fun alapapo adalu. A ti yọ iyọkuro akoko (250μl) kuro ni ojutu ṣaaju iṣaaju ifilọlẹ nipa ifikun ni nigbakanna ti hydro peroxide ati ohun elo ti awọn igbi ultrasonic. Hydrogen peroxide ti a ṣafikun lati gba ipin hydrogen peroxide / heparin (w / w) ikẹhin ti 0.15 (3.75 mg / milimita).
Iṣeduro ẹrọ:
UP50H pẹlu sonotrode MS3
Itọkasi / Iwe Iwadi:
Achour, Oussama; Bridiau, Nicolas; Godhbani, Azza; Le Joubioux, Florian; Bordenave Juchereau, Sitefanu; Sannier, Fredéric; Piotọn, Jean-Marie; Fruitier Arnaudin, Ingrid; Maugard, Thierry (2013): Ṣetọju iranlọwọ-Ultrasonic ti ẹjẹ heparin iwuwo kekere kan (LMWH) pẹlu iṣẹ anticoagulant. Awọn ọlọjẹ Carbohydrate 97; 2013. 684–689.

hops

Ohun elo Ultrasonic:
Ifaagun ti awọn akopọ ti nṣiṣe lọwọ / awọn afikun egboigi ninu omi ati ọti ẹmu.
Iṣeduro ẹrọ:
UP400S

Lactobacillus (ipinya / DNA ipinya ti awọn ọpọlọpọ igara Lactobacillus)

Ohun elo Ultrasonic:
Awọn okun Lactobacillus: L. pontis, L. sanfranciscensis, L. farciminis, L. panis, L. orí, L. vaginalis ati L. reuteri, L. sp.
Ilana: Fun ipinya DNA ti awọn ileto nikan, a ṣe agbekalẹ Ilana lysis ultrasonic. Ileto kan (iwọn ila opin 2-si 3-mm) ni ifasilẹ 100μl lysis buffer (20 mM EDTA, 10 mM Tris [pH 7.9], 1% Triton X-100, 500 mM guanidine-HCl, 250 mM NaCl). Wọn ṣe lilu awọn sẹẹli nipasẹ iṣẹju 1 ti ultrasonication pẹlu ẹrọ ti o ni iru ultrasonicator UP50H. Lẹhin afikun ti 150μl ti ethanol tutu (-20 ° C), a ti petele adalu naa lori iwe yiyi ti ohun elo t’ẹgbẹ QIAamp ati nikẹhin rẹ pẹlu 60μl ti ifipamọ (10 mM Tris [pH 7,5]).
Lati ni ọpa kan fun idanimọ iyara ati idanimọ ti awọn asa mimọ nikan, a ṣe idawọle PCR pẹlu ilana ipinya DNA iyara. Awọn ilana enzymatic lysis-akoko ati ailagbara oniyipada ti awọn kokoro arun si lysozyme ni a bori nipasẹ itọju ultrasonic ti awọn sẹẹli, pẹlu isọdọmọ ati ifọkanbalẹ nipa didi DNA si matrix kan. Ohun elo sẹẹli ti ileto kan ṣoṣo ni a rii lati to fun PCR.
Iṣeduro ẹrọ:
UP50H
Itọkasi / Iwe Iwadi:
Müller, MRA (2000): Abuda ti ilolupo makirowefu ti Awọn iṣu-ngun ti Oje nipa lilo Awọn ọna Awọn ọna Ige. Ile-iwe Itẹwe ti Cologne, 2000.

Lactobacillus acidophilus Gr +

Ohun elo Ultrasonic:
Ultraac inactivation ti Lactobacillus acidophilus Gr + ni wara ati awọn ọra-wara.
Iṣeduro ẹrọ:
UIP500hd
Itọkasi / Iwe Iwadi:
Zenker, M .; Heinz, V.; Knorr, D. (2003): Ohun elo ti olutirasandi iranlọwọ processing gbona fun itọju ati idaduro didara ti awọn ounjẹ omi. J Ounjẹ Prot 66/2003. p. 1642–1649.

Legionella pneumophila Gr + ni alabọde ti fomi po

Ohun elo Ultrasonic:
Ultrasonic inactivation ti Legionella pneumophila Gr + ni alabọde ti fomi po.
Iṣeduro ẹrọ:
UIP500hd
Itọkasi / Iwe Iwadi:
Dadjour, MF; Ogino, C .; Matsumura, S .; Nakamura, S .; Shimizu N. (2006): Disinfection ti Legionella pneumophila nipasẹ itọju ultrasonic pẹlu TiO2. Omi Res 40/2006. pp.1137–1142.

Leuconostoc mesenteroides

Ohun elo Ultrasonic:
Iṣe Leukocyte lysozme ni mimilocytic lukimia: a ti fi idaduro idaduro sẹẹli sẹẹli fun 15 min. ati awọn ayẹwo ti o lu fun iṣẹ lysozyme. Fojusi lysozyme ti awọn sẹẹli leukocytes ug./10 ni a ti pinnu.
Iṣeduro ẹrọ:
UP50H

Leukocytic aṣayan iṣẹ isozym ninu aisan lukimia myelocytic

Ohun elo Ultrasonic:
Igbaradi iṣapẹrẹ: idaduro sẹẹli jẹ itọju ati itọju awọn ayẹwo fun iṣẹ lysozyme. Ifojusi lysozyme ti leukocytes ug / 106 ni ipinnu.
Iṣeduro ẹrọ:
UP200St

Malachite Green

Ohun elo Ultrasonic:
Ibajẹ Sonophotocatalytic ti Malachite Green (ipakokoro to lagbara): Ibajẹ ibajẹ fọtocatalytic nikan ni iyara yiyara ju ibajẹ sonolytic, ṣiṣe le dara si nipasẹ ṣiṣepo awọn ilana mejeeji. Alawọ ewe Malachite, kokoro alamọ lagbara, jẹ ecotoxic pupọ si awọn kokoro arun to ni okun ṣugbọn o yipada si awọn ohun-ara ti o kere si tabi kii ṣe majele.
Iṣeduro ẹrọ:
UP400S

Mangiferin acylation

Ohun elo Ultrasonic:
Mangiferin (1,3,6,7-Tetrahydroxy-2- [3,4,5-trihydroxy-6- (hydroxymethyl) oxan-2-yl] xanthen-9-one; agbekalẹ: C19H18O11) jẹ polyphenol ti eto C-glycosylxanthone ti o le rii ni ọpọlọpọ awọn irugbin ọgbin. Mangiferin ṣafihan ọpọlọpọ awọn iṣẹ elegbogi. Ifọwọkan regioselective ti mangiferin le jẹ catalyzed daradara nipasẹ lipase labẹ ultrasonication. Ti a ṣe afiwe pẹlu awọn ọna ti apejọ, awọn iwadii catalysis ti ultrasonically ṣe iranlọwọ nipasẹ awọn anfani ti akoko igba kikuru ati awọn eso ti o ga julọ. Awọn ipo ti o dara julọ fun ifamọra mangiferin ultrasonic ni a rii bi atẹle:
lipase: PCL, oluṣeto acyl: acetate ti vinyl; Igbẹhin ti o ni idi: DMSO, iwọn otutu ti n ṣe ayẹwo: 45 degC, agbara ultrasonic: 200W; ipinnu substrate: acyl donor / mangiferin 6/1, ikojọpọ enzymu: 6 iwon miligiramu / milimita
Awọn ikẹkọ acylation regioselective jẹ to 84%.
Iṣeduro ẹrọ:
UP200St tabi UP200Ht
Itọkasi / Iwe Iwadi:
cp .: Wang, Z .; Wang, R .; Tian, J .; Zhao, B; Wei, XF; Su, YL; Li, CY; Cao, SG; Ọgbẹni Wang, L. (2010): Ipa ti olutirasandi lori lipid-catalyzed regyselective acylation ti mangiferin ni awọn ti kii-olomi solvents. J. Asian Nat Prod. Res. 12/1, 2010. 56-63.

Isediwon Molecules

Ohun elo Ultrasonic:
Ilana isediwon fun isediwon lati gypsum, rheolite, basalt, edfell ash ati gilasi obsidian:
Apejuwe 1g ti regolith tabi apata itemole jẹ koko-ọrọ lati isediwon omi labẹ irọndi ultrasonic lati jade ati awọn ohun-ini fojusi. Nitorinaa, 3ml MeOH P80 ti a ṣafikun si apẹẹrẹ analog 1g ni tube idanwo gilasi kan ati sonicated fun iṣẹju 20 pẹlu ẹrọ iru-ẹrọ iwadii ultrasonic UP50H ṣeto ni titobi 40%. A gba iyọnda naa duro fun iṣẹju 10, ati apọju awọsanma ti o ti ṣe agbekalẹ loke iṣapẹẹrẹ analanceed ti a ti pinnu sinu awọn iwẹfa milimita milimita 1.5 milimita. Lati dinku pipadanu awọn ohun elo ti a fa jade nipasẹ adsorption si awọn roboto ti awọn tubes hydrophobic polima centrifuge Falopiani, awọn Falopiani ti kọkọ dina pẹlu 0,5% (w / v) BSA ni 100 mM HEPES pH 7.4. Awọn eletan ti ṣalaye nipasẹ centrifugation ni 17000 G fun iṣẹju 10 ati pe wọn fipamọ ni 2 - 8 ° C ni awọn lẹmọọn gilasi titi ti a fi dán wọn ninu immunoassay.
Iṣeduro ẹrọ:
UP50H
Itọkasi / Iwe Iwadi:
Rix, C. (2012): Wiwa Igbesi aye lori Mars ati Chip Life Mark Chip: Awọn idiyele Antibody fun Ṣiṣawari Awọn Iṣii Organic ni Awọn ifaagun Awọn Liquid ti Awọn apẹẹrẹ Samian. Ifiweranṣẹ Cranfield University 2012.

Asin peli

Ohun elo Ultrasonic:
Awọn fo naa wẹ ati fifọ fun iṣẹju 5 pẹlu milimita 0,5 si siwaju ti LB2 ati centrifuged ni 12,000 g fun iṣẹju 20 miiran, ati pe idapọmọra awọn ida meji ti apọju ni a gba. Lakotan, awọn peli naa ni tituka pẹlu 0,5 milimita ti ifipamọ ti o ni ipilẹ 40 mM tris base, 5 M urea, 2 M thiourea, 4% CHAPS, 100 mM DTT, 0,5% (v / v) biolyte 3-10 (LB3), ati pe sonicated ati centrifuged ni 12,000 g fun 20 min.
Iṣeduro ẹrọ:
UP200S; fun 5min.
Itọkasi / Iwe Iwadi:
Gazzana et al. (2009): Imudojuiwọn lori proteme ẹdọ Asin.

Iduro ẹdọ

Ohun elo Ultrasonic:
Ayẹwo homogenization lati gbejade lysate sẹẹli.
Iṣeduro ẹrọ:
UP200S; fun 3 x 20 iṣẹju-aaya.
Itọkasi / Iwe Iwadi:
Gazzana et al. (2009): Imudojuiwọn lori proteme ẹdọ Asin.

Penicillium digitatum

Ohun elo Ultrasonic:
Ultrasonic inactivation ti Penicillium digitatum (egbogi ọgbin) ni alabọde idagbasoke Sabouraud.
Iṣeduro ẹrọ:
UP200St
Itọkasi / Iwe Iwadi:
López-Malo, A .; Palou, E.; Jiménez-Fernández, M .; Alzamora, SM; Guerrero, S. (2005): Inactivation ti ọpọlọpọ ti iṣelọpọ pọ pẹlu thermosonication ati awọn antimicrobials. J. Ounjẹ Eng. 67 / 2005. p. 87-99.

Phycocyanin lati Spirulina platensis (Arthrospira platensis)

Ohun elo Ultrasonic:
Ifaagun ti phycocyanin lati awọn sẹẹli Spirulina platensis (Arthrospira platensis).
Iṣeduro ẹrọ:
UP400S

Awọn sẹẹli ati ohun ọgbin

Ohun elo Ultrasonic:
30% awọn sẹẹli ọgbin ti o papọ (W / V) ati omi dist dist ti wa ni idilọwọ nipasẹ sonication fun 1-15 min.; idapọ ti àsopọ ọgbin: 1 g àsopọ ti o gbẹ ti daduro fun igba diẹ ninu ọti ti wa ni tituka lakoko sonication ti to 5 min.
Iṣeduro ẹrọ:
UP100H

platelets

Ohun elo Ultrasonic:
Platelet lysate igbaradi: Idalọwọduro ni 1-5 min.
Iṣeduro ẹrọ:
UP200Ht

Pleurotus tuberregium

Ohun elo Ultrasonic:
Awọn isediwon ti awọn polysaccharides lati inu epo ti Pleurotus funre jẹ
Iṣeduro ẹrọ:
UP400S

Poly (lactic-co-glycolic acid) (PLGA)

Ohun elo Ultrasonic:
Igbaradi ti bovine omi ara albumin (BSA) -tijọpọ poly (lactic-co-glycolic acid) (PLGA) nipasẹ sonication ni 40 iṣẹju-aaya.
Iṣeduro ẹrọ:
dmini; fun 40sec.
Itọkasi / Iwe Iwadi:
Freitas et al. (2005): Isan-nipasẹ ultrasonic emulsification ni idapo pelu mimiromicing stic fun aseptic gbóògì ti microspheres nipasẹ epo isediwon.

Awọn polyphenols lati apple

Ohun elo Ultrasonic:
Ultrasonic isediwon ti awọn polyphenols lati apple. Ojutu: olomi. Ilọsi pọ si nipasẹ 6%; kikankikan sonication: 20-75Ws / milimita; ilana temp.: kikan si 80 degC.
Iṣeduro ẹrọ:
UIP2000hd
Itọkasi / Iwe Iwadi:
Vilkhu, K .; Manasse, R .; Mawson, R .; Ashokkumar, M. (2011): Imularada Ultrasonic ati Imudara ti Awọn eroja ounjẹ. Ni: Feng / Barbosa-Cánovas / Weiss (2011): Awọn eroja olutirasandi fun Ounje ati Bioprocessing. New York: Springer, 2011. ni 345-368.

Awọn polyphenols lati tii dudu

Ohun elo Ultrasonic:
Ultrasonic isediwon ti awọn polyphenols lati tii dudu. Ojutu: olomi. Ilọsi pọ si nipasẹ 6-18%; kikankikan sonication: 8-10Ws / milimita; ibaramu ibaramu, tempeli ilana.: kikan si 90 degC.
Iṣeduro ẹrọ:
UIP2000hd
Itọkasi / Iwe Iwadi:
Vilkhu, K .; Manasse, R .; Mawson, R .; Ashokkumar, M. (2011): Imularada Ultrasonic ati Imudara ti Awọn eroja ounjẹ. Ni: Feng / Barbosa-Cánovas / Weiss (2011): Awọn eroja olutirasandi fun Ounje ati Bioprocessing. New York: Springer, 2011. ni 345-368.

Awọn polyphenols lati awọ-eso ajara pupa

Ohun elo Ultrasonic:
Ultrasonic isediwon ti awọn polyphenols lati awọ eso ajara pupa. Ojutu: olomi. Ilọsi pọ si nipasẹ 11-35%; kikankikan sonication: 20-75Ws / milimita; ibaramu titẹ.
Iṣeduro ẹrọ:
UIP2000hd
Itọkasi / Iwe Iwadi:
Vilkhu, K .; Manasse, R .; Mawson, R .; Ashokkumar, M. (2011): Imularada Ultrasonic ati Imudara ti Awọn eroja ounjẹ. Ni: Feng / Barbosa-Cánovas / Weiss (2011): Awọn eroja olutirasandi fun Ounje ati Bioprocessing. New York: Springer, 2011. ni 345-368.

Awọn polyphenols, amino acid ati kanilara lati tii alawọ

Ohun elo Ultrasonic:
Ifaagun ti awọn akopọ ti nṣiṣe lọwọ lati Tii alawọ ni omi
Iṣeduro ẹrọ:
UP400S

Ẹran ẹlẹdẹ: Pipọnti awọn ẹran ẹlẹdẹ

Ohun elo Ultrasonic:
Fun brining ti iranlọwọ ultrasonically, awọn ẹyẹ ẹlẹdẹ (Longissimus dorsi) ni a tẹ sinu omi iṣuu soda kiloraidi (40 g L − 1) ati mu ni 5 ° C pẹlu olutirasandi kekere-kekere (20 kHz) ni agbara-kekere (2 -4 W / cm −2). Ipa ti ultrasonic iranlọwọ isọdọtun lori microcine àsopọ, imunisin amuaradagba, agbara abuda omi (WBC), agbara mimu omi (WHC), iṣuu soda iṣuu soda sọtọ (D) ati lori profaili ọrọ eran (TPA). Awọn abajade fihan pe itọju ultrasonic fa awọn ayipada microstructural ti o wuyi ninu ẹran ara. Agbara mimu omi ati awọn ohun-ini ọrọ jẹ ilọsiwaju nipasẹ itọju ultrasonic akawe si mejeeji ṣubu ati awọn ayẹwo brined aimi. Sibẹsibẹ, awọn igbelaruge rere wọnyi jẹ igbẹkẹle igbẹkẹle ultrasonic kikankikan. Awọn ipa ti o ga ati / tabi awọn akoko itọju to gun to fa iye-ara ti awọn ọlọjẹ. Awoṣe oniyepupọ igbagbogbo a ni anfani lati ṣapejuwe parọto awọn ibatan iyasọtọ NaCl lakoko mimu bi mimu. Itọju Ultrasonic ṣe pataki kaakiri itankale ti iyọ ni akawe si awọn ayẹwo ti a tẹri labẹ awọn ipo aimi ati alafọwọpọ aladapọ pọ pẹlu pọsi kikankikan ultrasonic.
Iṣeduro ẹrọ:
UP200Ht pẹlu sonotrode S26d40
Itọkasi / Iwe Iwadi:
Siro, Mo.; Ven, Cs ;; Balla, Cs .; Jonas, G.; Zeke, Emi.; Friedrich, L. (2009): Ohun elo ti ilana itọju curing ultrasonic ti o ṣe iranlọwọ fun imudara itankale iṣuu soda iṣuu ni eran porcine. Iwe akosile ti Imọ-ẹrọ Ounjẹ 91/2, 2009. 353-362.

Porphyra yezoensis

Ohun elo Ultrasonic:
Ibajẹ abuku Ultrasonic ti awọn polysaccharides lati Porphyra yezoensis: 50mL ti 1.0 g / 100mL porphyra yezoensis polysaccarides (iwuwo gbigbẹ) ti jẹ ọmọ fun 4 wakati pẹlu ohun UP400S.
Iṣeduro ẹrọ:
UP400S; olutirasandi ti a fa jade (awọn kẹkẹ: 2 iṣẹju-aaya lori / 2 iṣẹju pa) ni 20 ° C.

Awọn lulú

Ohun elo Ultrasonic:
Lilọ, lilọ deagglomeration ati pipinka si kekere, iwọn ara patiku iwọn ara.
Iṣeduro ẹrọ:
UP200St

Egungun egungun

Ohun elo Ultrasonic:
Idalọwọduro ti 1 gm ni iṣẹju 5-7.
Iṣeduro ẹrọ:
UP200St

Ẹdọ didi

Ohun elo Ultrasonic:
Idalọwọduro ati homogenization àsopọ pẹlu ẹya UP400S.
Iṣeduro ẹrọ:
UP400S; Awọn akoko 3 fun 30 iṣẹju-aaya; lori yinyin.
Itọkasi / Iwe Iwadi:
Oh et al. (2003): Acetyl-CoA Carboxylase Gene Ti wa ni Iṣeduro nipasẹ Amuaradagba Elegu-ti o ni ibamu Amọdaju-1 ninu Ẹdọ.

Arun awọ

Ohun elo Ultrasonic:
Idalọwọduro ti 1 gm ni iṣẹju 1.
Iṣeduro ẹrọ:
UP400S

Rawolfia Serpentina

Ohun elo Ultrasonic:
Isediwon ti ifiomipamo alkaloid.
Iṣeduro ẹrọ:
UP100H

rebaudioside A

Ohun elo Ultrasonic:
Awọn ayẹwo ti 10g gbẹ ati ilẹ stevia leaves ni a fa jade ni 100mL omi labẹ lemọlemọfún agunsise (pẹlu kan stirrer magnitude). A ṣe iṣakoso pH iye pẹlu 0.01 M pH 7 sodium phosphate. A fi ayẹwo naa sinu adẹnti gilasi 150 mL kan ati pe o ṣe itọsi pẹlu ultrasonicator ultrasonic probe (UIP500hd, 20kHz, 500W). Awọn sample ti sonotrode a immersed nipa 1,5 cm sinu slurry ti awọn leaves stevia. Awọn ẹrọ ultrasonic ti ṣeto fun agbara wu jade ti 350W. Imọ itọju ọmọ soni ti 350 W fun 5-10 min. ni iwọn otutu ilana igbagbogbo ti 30 ° C fi fun un rebaudioside A ikore ti 30-34g fun 100g ayẹwo. Lẹhin ti sonication, awọn orisun jade ti a centrifuged ati ki o filtered pa nipasẹ 0.45 μm microporous awo ilu; a ti mu filtrate naa fun lapapọ rebaudioside A imọran akoonu. Isoro iyasoto ti lapapọ ti a ti dapọpọ A akoonu ti ṣawari nipasẹ HPLC.
Nipa iyasọtọ ti kii ṣe iyasọtọ ti kii ṣe nkan-didasilẹ, ọna giga ti rebaudioside A ni a gba ni afiwe si awọn ilana igbasilẹ ti ibile gẹgẹbi isediwon ooru tabi maceration.
Iṣeduro ẹrọ:
UIP500hd

sitashi iresi

Ohun elo Ultrasonic:
Idalọwọduro, ipinya sitashi, ati adehun awọn iwe ifowopamoto ti ko ni aabo laarin amuaradagba ati sitashi ni iyẹfun iresi kan (33%) ni 20 – 40 iṣẹju.
Iṣeduro ẹrọ:
UP500hd; 20 – 40 iṣẹju.

Rotifers

Ohun elo Ultrasonic:
Idarujẹ sẹẹli ti mesozooplankton: nipasẹ sonication labẹ awọn ipo atẹle ti awọn oṣuwọn sisan mẹrin (200, 400, 520 ati 800lh-1) ati awọn amplitudes mẹrin (25, 50, 75 ati 100%) oṣuwọn pipa kan laarin 58 ati 85% ti waye.
Iṣeduro ẹrọ:
UP2000 pẹlu sẹẹli sisan
Itọkasi / Iwe Iwadi:
Viitaslo et al. (2005): Ozone, Ultraviolet Light, Olutirasandi ati omi epo Bi awọn Ballast Omi itọju – Awọn idanwo pẹlu Mesozooplankton Ni Ẹwa Alaini- Omi Ipaju.

S. fragilis

Ohun elo Ultrasonic:
itusilẹ ti galactokinease ni iṣẹju 4.
Iṣeduro ẹrọ:
UP200St

Saccharomyces cerevisiae

Ohun elo Ultrasonic:
Idalọwọduro
Iṣeduro ẹrọ:
UP400S
Itọkasi / Iwe Iwadi:
Guerrero, S,; López-Malo, A .; Alzamora, SM (2001): Ipa ti olutirasandi lori iwalaaye ti Saccharomyces cerevisiae: ipa ti iwọn otutu, pH ati titobi. Innov. Sci Ounjẹ. Emerg Technol. 2/2001. p. 31-39.

Saccharomyces cerevisiae

Ohun elo Ultrasonic:
Ultrasonic inactivation ti Saccharomyces cerevisiae ni alabọde idagbasoke Sabouraud ati ni iyo.
Iṣeduro ẹrọ:
UP400S
Itọkasi / Iwe Iwadi:
Yap, A.; Jiranek, V .; Grbin, P .; Barnes, M .; Awọn Bates, D. (2007): Awọn ijinlẹ lori ohun elo ti awọn ultrasonics ti o ni agbara giga fun agba ati fifọ plank ati disinfection. Austr. Ile-iṣẹ Waini NZ. J. 22 (3) / 2007. p. 96-104.

Saffron, sisuvus crocus

Ohun elo Ultrasonic:
Ifaagun awọn akopọ ti nṣiṣe lọwọ (awọn eroja ati awọn aṣoju awọ).
Tẹ ibi lati ka diẹ sii nipa isediwon lati saffron!
Iṣeduro ẹrọ:
UP50H
Itọkasi / Iwe Iwadi:
Kadkhodaee et al. (2007): Isediwon Ultrasonic ti Awọn iṣiro Iṣeduro lati Saffron.

Salmonella Senftenberg 775W Gr-

Ohun elo Ultrasonic:
Ultraac inactivation ti Salmonella Senftenberg 775W Gr- ni McIlvaine citrate-fosifeti ajekii tabi omitooro ounjẹ.
Iṣeduro ẹrọ:
UP100H
Itọkasi / Iwe Iwadi:
Álvarez, I .; Manas, P .; Virto, R .; Condón, S. (2006): Inactivation ti Salmonella Senftenberg 775W nipasẹ awọn igbi ultrasonic labẹ titẹ ni awọn iṣẹ omi oriṣiriṣi. Int J. Microbiol Ounje. 108/2006. p. 218-225.

Salvia miltiorrhiza bunge

Ohun elo Ultrasonic:
Ifaagun ti awọn agbo ogun ti nṣiṣe lọwọ ti ibi: iṣuu soda Danshensu ati tanshinones mẹrin (dihydrotanshione I, tanshinone I, cryptotanshinone ati tanshinone IIA).
Iṣeduro ẹrọ:
UP100H

Salvia Officinalis

Ohun elo Ultrasonic:
Ifaagun ti awọn akopọ ti nṣiṣe lọwọ lati Salvia Officinalis (sage) ni o kere ju 2hr.
Iṣeduro ẹrọ:
UP50H

omi ara

Ohun elo Ultrasonic:
homogenization
Iṣeduro ẹrọ:
UP200Ht

Agutan cystic livers, ẹdọforo ati ẹjẹ rere (Echinococcus granulosus antigens)

Ohun elo Ultrasonic:
Agutan cystic livers, ẹdọforo ati ẹjẹ rere (Echinococcus granulosus antigens in ọdọ-agutan): A ṣe ayẹwo ayẹwo lẹhinna fun 2 × 15 iṣẹju lori yinyin titi ko fi han awọn ilana inu tootun.
Iṣeduro ẹrọ:
UP200S; 2 x 15 iṣẹju-aaya.
Itọkasi / Iwe Iwadi:
Tabar et al. (2009): Idahun ti eniyan lodi si iṣan omi hydatid, protoscolex ati gbogbo ara ti awọn antigensus Echinococcus granulosus ninu awọn ọdọ-agutan.

Sorbitant Trioleate, ethanol (bi epo) ati lulú Bi-2212

Ohun elo Ultrasonic:
Deagglomerate slurry kan ti o ni Sorquant Trioleate dispersant, ethanol (bi epo) ati lulú Bi-2212.
Iṣeduro ẹrọ:
UP200S; fun 3 min.
Itọkasi / Iwe Iwadi:
Mora et al. (2009): Iṣelọpọ ti Awọn aṣọ Coconingsing lori Awọn alẹmọ Ilẹ Ceramic.

Soy isoflavones

Ohun elo Ultrasonic:
isediwon ultrasonic ti isoflavones soy ninu omi ati epo. Alekun ti o pọ to 15% ni ṣiṣe isediwon.
Iṣeduro ẹrọ:
UP200St
Itọkasi / Iwe Iwadi:
Rostagno et al. (2003), ti a tọka si nipasẹ Vilkhu, K.; Mánásè, R.; Mawson, R .; Ashokkumar, M. (2011): Imularada Ultrasonic ati iyipada ti awọn eroja Ounje. Ninu: Feng / Barbosa-Cánovas / Weiss (2011): Awọn Imọ-ẹrọ olutirasandi fun Ounje ati Bioprocessing. Niu Yoki: Springer, 2011. p. 345-368.

Soy Protein

Ohun elo Ultrasonic:
Ultrasonic isediwon ti amuaradagba soyi ninu omi ati alkali (iṣuu soda sodaxide). Alekun pọ si nipasẹ 53%. Inline sonication ti a ri diẹ sii daradara bi ipele isediwon (opopo sonication fun kan 23% ga ikore ju ipele sonication).
Iṣeduro ẹrọ:
UIP1000hd fun ipele / beaker ati pẹlu sisan sẹẹli riakito fun inline sonication.
Itọkasi / Iwe Iwadi:
Moulton and Wang (1982), ti a tọka si nipasẹ Vilkhu, K.; Mánásè, R.; Mawson, R .; Ashokkumar, M. (2011): Imularada Ultrasonic ati iyipada ti awọn eroja Ounje. Ninu: Feng / Barbosa-Cánovas / Weiss (2011): Awọn Imọ-ẹrọ olutirasandi fun Ounje ati Bioprocessing. Niu Yoki: Springer, 2011. p. 345-368.

Olori ori

Ohun elo Ultrasonic:
Idalọwọduro ni iṣẹju 10.
Iṣeduro ẹrọ:
UP100H

Awọn iru omi ara

Ohun elo Ultrasonic:
Idalọwọduro lẹsẹkẹsẹ
Iṣeduro ẹrọ:
UP100H

Stevia rebaudiana Bert.

Ohun elo Ultrasonic:
Iyọkuro Ultrasonic ti stelyside glycoside lati awọn ayẹwo ti 10 g ti awọn eso gbigbẹ lati Stevia rebaudiana pẹlu awọn titobi patikulu mẹrin (0.315 mm, mm 2, 6.3 mm ati awọn igi gbigbẹ ti a ni itemole) ni a dapọ pẹlu awọn oriṣiriṣi oriṣiriṣi omi: omi ti a fi omi papọ ati awọn iparapọ omi ethanol (55% ati 70%) pẹlu iwuwo ayẹwo ti o yatọ lati yanju awọn iwọn iwọn iwọn: 1/10, 1/8, 1/5 (w / v) lẹhinna ti han si olutirasandi ni iwọn otutu yara. Akoko Sonication: < 5 min. Iṣeduro ẹrọ:
UP200St