Hielscher faka'ata tekinolosia

Fakalahi ultrasonically 'a e Partitioning 'a e toko tolu 'o e konga

  • Tolu-konga partitioning (TPP) ko ha founga ke to'o, fakamavahe'i mo fakama'a 'a e ngaahi konga, e.g lipids, 'enisaimi (enzyme), polysaccharides mo e biomolecules kehe, mei he fakamatala totonu.
  • Na'e ma'olunga taha e TPP anga-maheni 'aki 'oku 'omi ma'olunga ange, haohaoa kuo fakalelei'i pea vave makehe ha partitioning ki he toko tolu 'o e konga tokoni ultrasonically.
  • Ultrasonic TPP 'oku kaunga ki ai koe'uhi ko ha ngaahi tohi iiki mo lalahi pea lava ke faingofua na'e holofa ki hono ngaohi 'o e ngaue.

 

Partitoning 'a e toko tolu 'o e konga ultrasonic

Partitioning 'a e toko tolu 'o e konga

Ko ha founga faingofua mo lelei 'a e one-step ki he mavahevahe, fakama'a mo fakatahataha'i 'o biomolecules hange ko 'enisaimi (enzyme), lipids, polysaccharides mo e ha fua mei he fihi 'a e toto'i 'a e toko tolu-konga partitioning (TPP).
'Oku fakahoko e TPP 'aki hono tanaki atu fakahokohoko 'o ha pa'anga fe'unga 'o e masima (meimei ammonium sulphate) mo ha totongi hono mo'ua ai (pe m-butanol /tert-butanol). 'Oku faka'aonga'i 'a e salts hange ko ammonium sulfate 'i ha pau faitonunga ke hoko ai e polotiini. Ha totongi hono mo'ua ai hange ko m-Ko e tanaki atu 'a e butanol ke fa'u 'a e toko tolu-ko e konga lahi, pea ke to'o 'a e kalasiume ma'ulalo molecular mamafa hange ko e lipids, phenolics mo ha me'afo 'oku vaivai. Talu mei he m-butanol kuo ha poini ki he boiling ma'olunga, ka ko e ngaahi me'a 'oku si'isi'i ange velangofua 'i he ethanol mo e methanol, m-butanol ko e faito'o 'oku fie ma'u ki he TPP.
Hili 'a e faito'o 'o e fakamatala na'e to'o ta'efe'unga mo e decantation, 'oku vahevahe'i ai 'a e me'a kuo tuifio ki he konga kehekehe 'e tolu: e (totongi hono mo'ua 'i 'olungam-butanol) 'i he konga ha kalasiume 'ikai ko e taupotu taha, 'a ia 'oku mavahe mei he e ngaahi konga aqueous ma'ulalo ange ('oku 'i ai e fehangahangai kalasiume) 'aki ha polotiini interfacial hoko ai. 'Oku filifili vaeua 'a e polotini 'oku fie ma'u ki ha konga 'e taha mo e ngaahi me'a fakatupu 'uli polotini ki he ngaahi konga kehe. 'Oku fakatupu 'eni fakakonga pe fakama'a mo e fakatahataha'i 'o e polotiini. Ko ha amalgamation 'o e kosmotropic, salting 'a isotonic precipitation cosolvent mo e osmolytic 'o e polotini 'a e founga to'o hingoa.

Kole 'a e fakamatala




Fakatokanga'i ange 'etau Tu'utu'uni totonu fakatautaha.


TPP fakalelei'i ultrasonically

Partitioning ki ha toko tolu 'o e konga ultrasonic ma'olunga taha 'e he ko e lahi taha 'o e mo'ui ma'a, fakaakeake mo reduced ngaue ki he taimi.Sonication intensifies the TPP process significantly. Ultrasonically assisted TPP is successfully applied for the separation and purification of biomolecules. A wide range of biomoelcules have been isolated more efficiently in shorter time. For instance, enzymes from plants (e.g. bromelain, papain, invertase, polyphenol oxidase and trypsin inhibitors), animals (e.g. trypsin, α-chymotrypsin, chymosin, pepsin and luciferase) and microbes (e.g. lipase, α-amylase, α-galactosidase, serratiopeptidase, cyclodextrin glycosyltransferase, and fibrinolytic enzymes) were purified via ultrasonic TPP. An additional benefit of the ultrasonic TPP is the enhanced activity of various enzymes, resulting in apparent higher yields (>100%) since the targeted enzyme or protein precipitates out in the middle layer between the organic and aqueous phases.
'E lava ke faka'aonga'i ke ola lelei mo ha ngaahi sipinga kovi 'a e ultrasonic TPP pea lava ke faingofua na'e holofa.

Ultrasonically fakalelei'i 'e tolu e konga Partitioning (TPP)

Fakalakalaka partitioning 'aupito 'a e toko tolu 'o e konga 'a e sonication mo ola 'i he ma'olunga ange 'o e 'oku 'omi mo e mo'ui ma'a 'i ha taimi nounou ange ai 'a e founga.

Ngaahi lelei:

  • 'Oku 'omi ma'olunga ange
  • fakaakeake vave
  • ma'olunga 'o e haohaoa
  • fakahaofi 'o e taimi
  • 'ikai fakamole
  • faingofua ke lele mahu'inga
  • eco-fakakaume'a
  • ki he fale fakatotolo fakakemi mo e fa'a ngaue

 

Ko e ngaahi sipinga 'i he U.S-TPP

TPP ultrasonic ki he to'o hingoa 'o e astaxanthin

Ki he to'o hingoa 'o e astaxanthin (AX), ko e faito'o fakatahataha 'o e TPP mo e sonication mo e Hielscher UP400S 'Oku 'ikai ngata pe 'i he ans faingofua faingofua 'oku kaunga ki ai, ka 'oku toe lelei 'aupito foki 'a e founga ke fakamavahe'i 'a e AX mei he hangatamaki biomass 'o e Paracoccus NBRC 101723. 'E lava ke ke optimized 'a e ngaahi me'a hono fakalele 'o e sonication ke ma'u 'a e ngaahi ola 'o e to'o hingoa tokolahi lelei e kau. Ngaue'anga ultrasonic ngaahi fakangatangata auch ko e amplitude, taimi, mafana 'o e 'ea mo e ipu 'o e to'o hingoa (e size, fotunga) pea mo e tokolahi 'a e kihi'i konga si'i 'o e biomass, 'oku uesia e tukuange ultrasonic 'o e AX mei he biomass 'aupito. 'Oku 'omi 'e he ultrasonication 'o e biomass viviku 'a e ngaahi ola lelei ange ia 'i he TPP ultrasonic 'o biomass momoa ki he to'o hingoa mei he vave pea mo e lelei 'o e AX. Ngaahi ola 'a e TPP ultrasonic 'i ha 37% ma'olunga ange AX fakaakeake ange e to'o anga-maheni 'aki e totongi hono mo'ua. (cf. Chougle et 'Alama 2013)

TPP ultrasonic ki he mavahe 'a andrographolide mei he paniculata 'o e Andrographis

Andrographolide ko ha diterpenoid 'o e labdane, 'a ia 'oku mahu'inga ki hono ma'olunga 'o e anti-inflammatory, antioxidant, hepatoprotective, anti-carcinogenic mo e diabetic fakafepaki ki he Mamonga 'a e ngaahi nunu'a. 'Oku fakahaa'i 'e he ako kehe.23 'Oku ultrasonic TPP 'oku 'ikai ngata pe tupulaki 'a e 'omi 'o e andrographolide ka 'oku toe fakalakalaka foki he 'ekitiviti antioxidative 'o fakatatau ki he anga-maheni 'o e TPP.

Ko e ngaue ma'olunga Ultrasonic Processors

'Oku makehe 'a e Hielscher Ultrasonics 'i he ko e palani mo e ngaohi 'o ha ngaahi polokalama ultrasonic high-power ki he to'o hingoa pea mo ha ngaahi founga lahi kehe.
Falute 'e 'a iiki, 'a 'etau ngaahi koloa ngaahi me'angaue loki fakasaienisi malohi, longomo'ui sea-'olunga ultrasonicators mo e ngaahi founga ngaue 'a e ma'olunga. E manifold Naunau faka'ata 'a e adaption lelei taha ia 'o e system ultrasonic ki ho'o ngaahi fie ma'u 'o e ngaahi founga pau. 'Oku langa 'a e ultrasonicators 'o e Hielscher kotoa pe ki he tafa 24/7 kavenga kakato.
'Oku faka'ata 'e he 'etau a'usia he'ene felave'i 'i he ngaue'anga ultrasonic kitautolu ke feongoongoi 'a e 'emau kau kasitomaa mei honau 'uluaki 'o e ngaahi sivi mo e ha 'i he ngaahi sitepu 'o e founga 'o e optimization mo hono fakahoko 'i he ngaue 'a e me'afua.
Kataki 'o ngaue 'aki e foomu 'i lalo ke fakamatala mai fekau'aki mo ho'o founga! 'Oku tau fiefia ke alea'i e ngaahi faingamalie mo e ngaahi lelei 'o e sonication ki ho'o tohi kole 'a e lekomeni.

Fetu'utaki mai kiate kimautolu! / Kole kiate kitautolu!

Kataki 'o faka'aonga'i e foomu 'i lalo, kapau 'oku ke loto ke kole ha fakamatala lahi ange fekau'aki mo e ultrasonic homogenization. Te tau fiefia ke 'oatu ha polokalama ultrasonic fakataha ho'o ngaahi fie ma'u.









Kataki 'o fakatokanga'i ange 'etau Tu'utu'uni totonu fakatautaha.


'Oku falute 'e he Hielscher 'a e kakato e vaha'a mei he fale fakatotolo fakakemi ma'opo'opo ultrasonicators sea-'olunga 'a e lahi mo e ngaahi polokalama lahi 'a e kakato hono ngaohi.

Malohi mo falala'anga ultrasonic naunau mei loki fakasaienisi ki he ngaue 'a e me'afua.

Ngaahi fakamo'oni fakafolofola/ngaahi tohi

  • Chougle, J.A: et 'Alama (2013): Na'e tokoni'i 'e ha toko tolu e konga partitioning (UATPP): fakatupulaki 'a e founga 'oku talanoa ki he to'o hingoa 'o e astaxanthin mei he Paracoccus NBRC 101723. CSBE/SCGAB 2013 – Fakata'u konifelenisi 'Univesiti 'o Sasikatesiuani, Saskatoon, 7-10 Siulai 2013.
  • Ketnawa, S. et al (2017): konga partitioning ki he enzyme mavahevahe: ko ha vakai fakalukufua mo e ngaahi polokalama fakamuimuitaha. Me'akai fakavaha'apule'anga e fekumi tohinoa 24(1), 2017. 1-24.
  • Varakumar, S. et 'Alama (2017): fakatupulekina to'o e 'uli 'o e oleoresin mei he Sinisa (Zingiber officinale) rhizome efuefu hono faka'aonga'i 'o e tokoni 'i he enzyme 'oku tolu konga partitioning. Me'akai fakakemi 216, 2017. 27 – 36.
  • Yan J.-K. et 'Alama (2018): synergized mo e partitioning 'a e toko tolu 'o e konga ki he to'o hingoa mo e mavahevahe 'o e Corbicula fluminea polysaccharides mo e ngaahi 'aonga 'e lava 'a e ha. Ultrasonics Sonochemistry Vol. 40, konga A, 2018. 128-134.


Ngaahi mo'oni'i me'a 'oku mahu'inga ke 'ilo'i

Partitioning (TPP) 'a e toko tolu 'o e konga

Ko ha founga bioseparation, 'a ia na'e makatu'unga 'i he partitioning 'o e taupotu taha 'o e kau poupou, polotini mo 'otometiki hydrophobic 'i he tolu tapa kehekehe kau ai ha vai, ammonium sulphate mo e m-butanol 'a e TPP.
Manifold mahu'inga 'oku fakama'a kitautolu 'e he fakafiemalie mo e fa'u 'enisaimi (enzyme) (e.g. α-galactocidase, α-amylase inhibitors mo e protease) mo e kalasiume fakanatula (e.g. diterpenes hange ko forskolin mo e andrographolide) 'e partitioning ki he konga 'e tolu. Hota pigments, lipids mo e enzyme inhibitors tanaki 'i he konga 'i 'olunga 'o e totongi hono mo'ua, 'a ia ko e fakamavahe'i mei he konga aqueous ma'ulalo ange, 'o hange ai ko e taupotu taha 'o e ngaahi konga saccharides 'oku tanaki, ha polotiini fakakonga precipitated pe e ki'i. Talu mei he m-butanol ne ha'i 'a e precipitated faka-TPP polotini, 'a ia 'oku hoko ko ha polotiini-k-butanol coprecipitates, ia floats 'i he vaha'a 'o fakatoka atu 'a e koa fufulu mo aqueous pea 'e lava ke faingofua hono fakamavahe'i pea fakahaohaoa'i. Ko hono fakalukufua, 'oku sai 'a e ngaahi biomolecules 'i ha foomu fakama'a 'i he interphase, lolotonga e ngaahi 'uli lahi taha hono vahevahe ki he m-butanol (ko e konga ki 'olunga) pea mo e konga aqueous (ko e konga ki lalo).

Astaxanthin

Astaxanthin (3, 3´-dihydroxy-ß, ß-carotene 4, 4´-dione) is a keto-carotenoid that belongs to the class of terpenes. As tetraterpenoid it is built from five carbon precursors, isopentenyl diphosphate, and dimethylallyl diphosphate. Astaxanthin is a yellow-orange plant pigmant and therefore classified as a xanthophyll. Astaxanthin is mainly found in microalgae, yeast, salmonoids, trout, krill, shrimp, crayfish, and crustaceans. As an highly potent free radical scavenger, skin protectant, anticarcinogenic and as an enhancer of immune system, astaxanthin is used as supplement and as additive in foods, pharmaceuticals and cosmetics. Ultrasonic TPP improves the extraction and separation process of astaxanthin from natural sources and makes its economical production applicable to industrial level.

'Oku makehe 'a e Hielscher Ultrasonics 'i he ngaue'anga ultrasonic ma'olunga

UIP1000hdT – ultrasonicator lalahi 'o e 1kW