Microtiter peleti ko e sipinga ʻo e teuteu naʻe fai ʻe Ultrasonication ʻOku fakaʻaongaʻi ʻa e ʻu lauʻi peleti Microtiter, ʻu lauʻi peleti fakavahaʻa-taʻu, 96 pe ʻu lauʻi peleti ʻa ʻELISA ki he sipinga lahi ʻo hono tanumaki mo e ngaue ki he ngaahi sipinga totonu. ʻI he peleti microtiter ultrasonicator UIP400MTP, ʻoku ʻomi ai ʻe Hielscher ha founga makehe ultrasonic ʻo e teunga mo e sipinga falalaʻanga ʻo hono teuteuʻi ʻo e ʻu lauʻi peleti lelei ʻe 96. ʻOku kau ʻi hono fakaʻaongaʻi angamaheni ʻo e microtiter ultrasonicator UIP4000MTP ʻa e lysis toʻotoʻo, ivi toʻo ʻuli, homogenization toʻotoʻo mo e solubilization pea pehe ki he DNA fragmentation. Sipinga lahi ʻo e teuteu mo e Ultrasonicator UIP400MTP Ko e ʻu lauʻi peleti Microtiter mo e ʻu lauʻi peleti lelei ʻe 96 ko ha meʻangaue ia ki hono tokangaʻi mo ngaue ʻi ha ngaahi sipinga lahi. ʻI heʻene hoko ko hono hingoa ko e "peleti lelei ʻe 96" kuo ʻosi fakaha mai, ʻoku ʻi ai ha ngaahi peleti vaitupu ʻe 96 pea ʻe lava ke maʻu ai ha sipinga fakafoʻituitui ʻe 96. ʻOku fie maʻu ha sipinga ʻo e teuteu kimuʻa pea toki ʻanalaiso, hange ko e lea toʻotoʻo (hange ko ʻELISA pe PCR) ki he lysis toʻotoʻo, fakahohaʻasi toʻotoʻo pea pehe ki hono toʻo e ʻuli mo hono tukuange ʻo ha intracellular molecules kehe. Ko e ultrasonic toʻotoʻo lysis mo e toʻo ʻuli ko ha founga taimi loloa mo fokotuʻu lelei ia ʻo e sipinga teuteu lelei. ʻOku fakahoko ʻa e ultrasonic toʻotoʻo lysis mo e ivi toʻo ʻuli ʻo fakatatau mo e ngaahi ola ʻo e repeatable mo falalaʻanga. Ko e sipinga lahi ultrasonicator UIP400MTP ko ha 400 Watts malohi ultrasonic ʻoku ʻi ai ha ngaahi peau ultrasonic ʻi he ngaahi holisi ʻo e ʻu lauʻi peleti fakavahaʻa-taʻu ki he sipinga Lotoloto. ʻOku sonicated ʻa e sipinga takitaha ʻe 96 ʻo e ngaahi vaitupu ʻaki ʻa e malohi tatau ultrasonic ʻi he lysis toʻotoʻo mo e toʻo hingoa. Ngaahi lelei ʻo e UIP400MTP MassSample Ultrasonicator Teunga sonication ʻo ha sipinga ʻe 96 Hoko ha sipinga ʻo e teuteu Ko hono mapuleʻingofua sonication fakangatangata (amplitude, loloa, siakale, mafana) Reproducible, repeatable ola Tafa faingamalie mo malu Ko e UIP400MTP ki he ngaahi lauʻi peleti ʻoku ʻikai fakahangatonu, ka ko e sonication lahi ʻo e ʻu lauʻi peleti Micro. Kole 'a e fakamatala Hingoa Tu'asila 'i-meili (fie ma'u) koloa pe faka'elia 'o e tokanga Fakatokanga'i ange 'etau Tu'utu'uni totonu fakatautaha. Kole ha fakamatala Mapuleʻi totonu ʻo e ʻea lolotonga Microtiter peleti Sonication Ko e UIP400MTP ko ha 400 Watts malohi ultrasonicator, ʻa ia ʻoku ne ʻave fakaʻata ngaahi peau mo e ko ha tetetete ʻi he ngaahi holisi ʻo e peleti microtiter ki he sipinga pea fakatupu cavitation mo e ngaahi malohi ʻo e kili ʻi he founga Lotoloto. ʻI he hoko ʻa e ngaahi founga fakahohaʻasi fakamisini kotoa pe, ʻoku fakatupu ʻe he sonication ha vela. Neongo ia, ʻi hono fakafehoanaki atu ki he ngaahi founga fakahohaʻasi toʻotoʻo, ʻoku fakaʻata ʻe he UIP400MTP ʻa e mafana ʻo e ʻea mo e ngaahi fakangatangata ʻo e ʻea ke taʻofi ha faʻahinga tuʻunga maʻulalo ʻo e ngaahi sipinga totonu. ʻOku fakanaunau ʻa e UIP400MTP ʻaki ha polokalama fakakomipiuta poto. ʻI he screen ki he naunau fakaʻilekitulonika, ʻe lava ke faingofua hono maʻu e menu ki he ngaahi fakangatangata ʻo e ʻea kimuʻa pea mo e polokalama fakakatoa ʻo e ivi ki hoʻo sipinga lele. Vakaiʻi e sipinga ʻo e ʻea: ʻOku fakanaunau ʻa e MultiSample Ultrasonicator UIP400MTP ʻaki ha polokalama fakakomipiuta fakapotopoto mo ha ʻea pluggable sensor resistance. Palaki ʻa e mafana ʻo e ʻea sensor resistance ki he UIP400MTP pea fakahu ʻa e tokoni ʻa e ʻea sensor resistance ʻi he taha ʻo e ngaahi vaitupu microtiter. Te ke lava ʻo fokotuʻu ʻi he menu ʻo e UIP400MTP ha mafana pau ki hoʻo sipinga sonication, ʻo fakafou ʻi he digital coloured Touch. ʻE taʻofi leva ʻa e ultrasonicator ʻi he taimi ʻe aʻu ai ʻa e mafana ʻo e ʻea pea kiʻi malolo kae ʻoua kuo aʻu ʻa e sipinga ʻo e ʻea ki he tuʻunga ʻulungaanga mahuʻinga maʻulalo ange ʻo e mafana ʻo e ʻ∆ mafana. Pea toe kamata leva ʻa e sonication. ʻOku taʻofi ʻe he founga fakapotopoto ko ʻeni ʻa e maʻulalo ʻoku ne fakaʻaiʻai ʻa e ʻea. Kapau ʻe lava, te ke lava ʻo tomuʻa fakaʻofoʻofa ʻa e peleti microtiter mo hono ngaahi sipinga ke fakatoloi hono aʻu ki he fakangatangata ʻo e ʻea mahuʻinga. UIP400MTP ki microtiter peleti/96-peleti lelei sonication UIP400MTP MassSampleUltrasonicator ʻOku fakaʻaongaʻi ʻe he kau fakatau fakaemamani lahi ʻa e Hielscher UIP400MTP ki he teuteu falalaʻanga, reproducible mo faingamalie ʻo e ngaahi sipinga lahi ʻi he ʻu lauʻi peleti fakavahaʻa-taʻu. ʻOku fakafaingofuaʻi ʻe he UIP400MTP ʻenau ngaue fakaʻaho ʻi he totonu, biochemical, saienisi ʻo e moʻui, fakafaitoʻo mo e kiliniki laboratories. ʻI hono puleʻi ko ia ʻo e polokalama fakakomipiuta mo e mafana ʻo e Hielscher UIP400MTP, ʻoku reliably mapuleʻi ai ʻa e ʻea mo e sipinga mafana ʻo e ʻea. ʻOku hanga ʻe ultrasonic sipinga ʻo e teuteu mo Hielscher Ultrasonics ʻ UIP400MTP ʻo fakahaofi ha ngaahi ola falalaʻanga mo reproducible! Kole ki hoʻo fakamatala taʻetotongi kau ai ʻa e ngaahi tohi kole mo e totongi ki he UIP400MTP ke fakafonu ʻa e fomu ʻi lalo pe fetuʻutaki mai kiate kimautolu ʻi he ʻimeili pe telefoni he ʻaho ni! Kumi ha founga ultrasonic ke ngaue ʻaki ha ngaahi tohi maʻolunga ange? 'Oku 'omi 'e he e tepile 'i lalo ha faka'ilonga ia 'o e tu'unga 'o e ngaue ki he fakafuofua'i 'o e hotau ultrasonicators: ʻOku ʻoatu ʻe he tepile ʻi lalo ha fakaʻilonga ʻo e fakafuofua ki he tuʻunga ʻo ʻetau ngaue ultrasonic mei he nima homogenizers mo MultiSample Ultrasonicators ki he ngaahi ultrasonic processors ki he ngaahi polokalama fakakomesiale: Kulupu (batch) 'o e tohi 'Oku tafe mai 'a e 'ea 'Oku fokotu'u atu 'a e ngaahi me'angaue 96-ngaahi lauʻi peleti/microtiter n.a. UIP400MTP 10 à 0.5 ki he 1.5 mL n.a. VialTweeter 'i he UP200St 0.01 ki he 250mL 5 ki he 100mL/miniti UP50H 0.01 ki he 500mL 10 ki he 200mL/miniti 'e UP100H mL 'i he 10 ki he 2000 20 ki he 400mL/miniti 'e UP200Ht, UP400St 0.1 ki he 20L 0.2 ke 4L/miniti 'e UIP2000hdT 10 ki he 100L 2 ki he 10L/miniti 'e UIP4000hdT n.a. 10 ki he 100L/miniti 'e UIP16000 n.a. lalahi fakataha'i 'o e UIP16000 Fetu'utaki mai kiate kimautolu! / Kole kiate kitautolu! Kole ki ha fakamatala lahi ange Kataki 'o ngaue 'aki e foomu 'i lalo ke kole ha toe fakamatala ki he ultrasonic processors, faka'aonga'i mo e totongi. Te mau fiefia ke alea'i e founga 'oku mo 'i ai mo ho'omou ngaahi fie ma'u ultrasonic he polokalama! Hingoa Kautaha Tu'asila 'i-meili (fie ma'u) Fika telefoni Tu'asila Kolo, vahefonua, kouti fakapositi 'ofisi Fonua Tokanga Kataki 'o fakatokanga'i ange 'etau Tu'utu'uni totonu fakatautaha. Kole ha fakamatala UIP400MTP ki microtiter peleti/peleti fakavahaʻa-taʻu sonication Ngaahi tohi/fakamo'oni fakafolofola Chen J.-X; Cipriani P.G.; Mecenas D.; Polanowska J.; Piano F.; Gunsalus K.C.; Selbach M. (2016): In Vivo Interaction Proteomics in Caenorhabditis elegans Embryos Provides New Insights into P Granule Dynamics. Molecular & Cellular Proteomics 15.5; 2016. 1642-1657. LeThanh, H.; Neubauer, P.; Hoffmann, F. (2005): The small heat-shock proteins IbpA and IbpB reduce the stress load of recombinant Escherichia coli and delay degradation of inclusion bodies. Microb Cell Fact 4, 6; 2005. Martínez-Gómez A.I.; Martínez-Rodríguez S.; Clemente-Jiménez J.M.; Pozo-Dengra J.; Rodríguez-Vico F.; Las Heras-Vázquez F.J. (2007): Recombinant polycistronic structure of hydantoinase process genes in Escherichia coli for the production of optically pure D-amino acids. Appl Environ Microbiol. 73(5); 2007. 1525-1531. Kotowska M.; Pawlik K.; Smulczyk-Krawczyszyn A.; Bartosz-Bechowski H.; Kuczek K. (2009): Type II Thioesterase ScoT, Associated with Streptomyces coelicolor A3(2) Modular Polyketide Synthase Cpk, Hydrolyzes Acyl Residues and Has a Preference for Propionate. Appl Environ Microbiol. 75(4); 2009. 887-896. Fekau'aki mo e ngaahi fu'u pou Lysis toʻotoʻo ʻo bl21 ngaahi selo ʻe Ultrasonication ʻOku pehe ʻe ultrasonic sipinga naʻe teuteuʻi maʻa ʻELISA As Sipinga ʻo e teuteu mo e Ultrasonic VialTweeter Protocol ki he SARS-KoloV-2 Coronavirus fakamalohia mo Sonication Ko e DNA ultrasonic kosi e fulufulu'i Ultrasonic Lysis 'o e Coli e. Ngaahi mo'oni'i me'a 'oku mahu'inga ke 'ilo'i Ko e ha ʻa microtiter ʻu lauʻi peleti? ʻOku toe ʻiloa foki ʻa e ʻu lauʻi peleti Microtiter, ʻa ia ʻoku toe ʻiloa ko e ʻu lauʻi peleti lelei ʻe 96, 96 microplates, ʻu lauʻi peleti fakavahaʻa-taʻu pe ngaahi peleti ʻa ʻELISA, ʻa ia ʻoku fakaʻaongaʻi lahi ʻi he laboratories ke ngaue ki he ngaahi selo, sipinga mopaila. ʻOku angamaheni ʻaki hono fakaʻaongaʻi ʻa e ʻu lauʻi peleti Microtiter ʻi manifold ngaahi lea hange ko ʻELISA mo PCR. Ko e lahi taha ʻo e ngaahi peleti lelei ʻe 96 ʻoku fakataumuʻa ia ki he throuput maʻolunga workflows pea ʻoku fenapasi ia mo ngaahi meʻalea ngaahi meʻa mo e ngaue angamaheni mo e ngaue. Ko ia ʻoku lahi ai e fakakeheʻi hange ko skirted ʻu lauʻi peleti ʻoku feʻunga mo e ngaahi platforms angamaheni, ʻikai skirted, Semi skirted, mo e ngaahi lauʻi peleti ʻoku liliu ki he sipinga ʻo e ngaue ʻoku lava ke maʻu. ʻOku kau ʻi hono fakaʻaongaʻi angamaheni ʻo e ʻu lauʻi peleti microtiter ʻa e ngaahi founga fakaʻaongaʻi hange ko e sipinga ʻo e tanaki, teuteu komipauni, combinatorial e kemisitulii, sivi maʻolunga throughput, nucleic fakamaʻa ʻo e ʻesiti, hangatamaki tupulaki fakafonua, mo e peleti replication.
Ngaahi tohi/fakamo'oni fakafolofola Chen J.-X; Cipriani P.G.; Mecenas D.; Polanowska J.; Piano F.; Gunsalus K.C.; Selbach M. (2016): In Vivo Interaction Proteomics in Caenorhabditis elegans Embryos Provides New Insights into P Granule Dynamics. Molecular & Cellular Proteomics 15.5; 2016. 1642-1657. LeThanh, H.; Neubauer, P.; Hoffmann, F. (2005): The small heat-shock proteins IbpA and IbpB reduce the stress load of recombinant Escherichia coli and delay degradation of inclusion bodies. Microb Cell Fact 4, 6; 2005. Martínez-Gómez A.I.; Martínez-Rodríguez S.; Clemente-Jiménez J.M.; Pozo-Dengra J.; Rodríguez-Vico F.; Las Heras-Vázquez F.J. (2007): Recombinant polycistronic structure of hydantoinase process genes in Escherichia coli for the production of optically pure D-amino acids. Appl Environ Microbiol. 73(5); 2007. 1525-1531. Kotowska M.; Pawlik K.; Smulczyk-Krawczyszyn A.; Bartosz-Bechowski H.; Kuczek K. (2009): Type II Thioesterase ScoT, Associated with Streptomyces coelicolor A3(2) Modular Polyketide Synthase Cpk, Hydrolyzes Acyl Residues and Has a Preference for Propionate. Appl Environ Microbiol. 75(4); 2009. 887-896.