Microtiter peleti ko e sipinga ʻo e teuteu naʻe fai ʻe Ultrasonication
Sipinga lahi ʻo e teuteu mo e Ultrasonicator UIP400MTP
Ko e ʻu lauʻi peleti Microtiter mo e ʻu lauʻi peleti lelei ʻe 96 ko ha meʻangaue ia ki hono tokangaʻi mo ngaue ʻi ha ngaahi sipinga lahi. ʻI heʻene hoko ko hono hingoa ko e "peleti lelei ʻe 96" kuo ʻosi fakaha mai, ʻoku ʻi ai ha ngaahi peleti vaitupu ʻe 96 pea ʻe lava ke maʻu ai ha sipinga fakafoʻituitui ʻe 96. ʻOku fie maʻu ha sipinga ʻo e teuteu kimuʻa pea toki ʻanalaiso, hange ko e lea toʻotoʻo (hange ko ʻELISA pe PCR) ki he lysis toʻotoʻo, fakahohaʻasi toʻotoʻo pea pehe ki hono toʻo e ʻuli mo hono tukuange ʻo ha intracellular molecules kehe. Ko e ultrasonic toʻotoʻo lysis mo e toʻo ʻuli ko ha founga taimi loloa mo fokotuʻu lelei ia ʻo e sipinga teuteu lelei. ʻOku fakahoko ʻa e ultrasonic toʻotoʻo lysis mo e ivi toʻo ʻuli ʻo fakatatau mo e ngaahi ola ʻo e repeatable mo falalaʻanga. Ko e sipinga lahi ultrasonicator UIP400MTP ko ha 400 Watts malohi ultrasonic ʻoku ʻi ai ha ngaahi peau ultrasonic ʻi he ngaahi holisi ʻo e ʻu lauʻi peleti fakavahaʻa-taʻu ki he sipinga Lotoloto. ʻOku sonicated ʻa e sipinga takitaha ʻe 96 ʻo e ngaahi vaitupu ʻaki ʻa e malohi tatau ultrasonic ʻi he lysis toʻotoʻo mo e toʻo hingoa.
- Teunga sonication ʻo ha sipinga ʻe 96
- Hoko ha sipinga ʻo e teuteu
- Ko hono mapuleʻingofua sonication fakangatangata (amplitude, loloa, siakale, mafana)
- Reproducible, repeatable ola
- Tafa faingamalie mo malu
The UIP400MTP for the indirect, yet intense sonication of micro-well plates.
Mapuleʻi totonu ʻo e ʻea lolotonga Microtiter peleti Sonication
The UIP400MTP is a 400 watts powerful ultrasonicator, which transmits ultrasound waves and vibrations indirectly through the walls of the microtiter plate into the samples and creates cavitation and shear forces in the medium. As all mechanical cell disruption methods, sonication creates heat. However, in contrast to alternative cell disruption methods, the UIP400MTP allows for sophisticated temperature monitoring and pre-setting of temperature limits to prevent any thermal degradation of the biological samples.
ʻOku fakanaunau ʻa e UIP400MTP ʻaki ha polokalama fakakomipiuta poto. ʻI he screen ki he naunau fakaʻilekitulonika, ʻe lava ke faingofua hono maʻu e menu ki he ngaahi fakangatangata ʻo e ʻea kimuʻa pea mo e polokalama fakakatoa ʻo e ivi ki hoʻo sipinga lele.
Vakaiʻi e sipinga ʻo e ʻea: ʻOku fakanaunau ʻa e MultiSample Ultrasonicator UIP400MTP ʻaki ha polokalama fakakomipiuta fakapotopoto mo ha ʻea pluggable sensor resistance. Palaki ʻa e mafana ʻo e ʻea sensor resistance ki he UIP400MTP pea fakahu ʻa e tokoni ʻa e ʻea sensor resistance ʻi he taha ʻo e ngaahi vaitupu microtiter. Te ke lava ʻo fokotuʻu ʻi he menu ʻo e UIP400MTP ha mafana pau ki hoʻo sipinga sonication, ʻo fakafou ʻi he digital coloured Touch. ʻE taʻofi leva ʻa e ultrasonicator ʻi he taimi ʻe aʻu ai ʻa e mafana ʻo e ʻea pea kiʻi malolo kae ʻoua kuo aʻu ʻa e sipinga ʻo e ʻea ki he tuʻunga ʻulungaanga mahuʻinga maʻulalo ange ʻo e mafana ʻo e ʻ∆ mafana. Pea toe kamata leva ʻa e sonication. ʻOku taʻofi ʻe he founga fakapotopoto ko ʻeni ʻa e maʻulalo ʻoku ne fakaʻaiʻai ʻa e ʻea.
Kapau ʻe lava, te ke lava ʻo tomuʻa fakaʻofoʻofa ʻa e peleti microtiter mo hono ngaahi sipinga ke fakatoloi hono aʻu ki he fakangatangata ʻo e ʻea mahuʻinga.
UIP400MTP ki microtiter peleti/96-peleti lelei sonication
UIP400MTP MassSampleUltrasonicator
Customers worldwide use the Hielscher UIP400MTP for the reliable, reproducible and convenient preparation of mass samples in multi-well plates. The UIP400MTP facilitates their daily work in biological, biochemical, life science, medical and clinical laboratories. With the intelligent software and temperature control of the Hielscher UIP400MTP, temperature is reliably controlled and heat-induced sample degradation avoided. Ultrasonic sample preparation with Hielscher Ultrasonics’ UIP400MTP delivers highly reliable and reproducible results!
Kole ki hoʻo fakamatala taʻetotongi kau ai ʻa e ngaahi tohi kole mo e totongi ki he UIP400MTP ke fakafonu ʻa e fomu ʻi lalo pe fetuʻutaki mai kiate kimautolu ʻi he ʻimeili pe telefoni he ʻaho ni!
Kumi ha founga ultrasonic ke ngaue ʻaki ha ngaahi tohi maʻolunga ange? 'Oku 'omi 'e he e tepile 'i lalo ha faka'ilonga ia 'o e tu'unga 'o e ngaue ki he fakafuofua'i 'o e hotau ultrasonicators:
ʻOku ʻoatu ʻe he tepile ʻi lalo ha fakaʻilonga ʻo e fakafuofua ki he tuʻunga ʻo ʻetau ngaue ultrasonic mei he nima homogenizers mo MultiSample Ultrasonicators ki he ngaahi ultrasonic processors ki he ngaahi polokalama fakakomesiale:
| Kulupu (batch) 'o e tohi | 'Oku tafe mai 'a e 'ea | 'Oku fokotu'u atu 'a e ngaahi me'angaue |
|---|---|---|
| 96-ngaahi lauʻi peleti/microtiter | n.a. | UIP400MTP |
| 10 à 0.5 ki he 1.5 mL | n.a. | VialTweeter 'i he UP200St |
| 0.01 ki he 250mL | 5 ki he 100mL/miniti | UP50H |
| 0.01 ki he 500mL | 10 ki he 200mL/miniti 'e | UP100H |
| mL 'i he 10 ki he 2000 | 20 ki he 400mL/miniti 'e | UP200Ht, UP400St |
| 0.1 ki he 20L | 0.2 ke 4L/miniti 'e | UIP2000hdT |
| 10 ki he 100L | 2 ki he 10L/miniti 'e | UIP4000hdT |
| n.a. | 10 ki he 100L/miniti 'e | UIP16000 |
| n.a. | lalahi | fakataha'i 'o e UIP16000 |
Fetu'utaki mai kiate kimautolu! / Kole kiate kitautolu!
UIP400MTP ki microtiter peleti/peleti fakavahaʻa-taʻu sonication
Ngaahi tohi/fakamo'oni fakafolofola
- Chen J.-X; Cipriani P.G.; Mecenas D.; Polanowska J.; Piano F.; Gunsalus K.C.; Selbach M. (2016): In Vivo Interaction Proteomics in Caenorhabditis elegans Embryos Provides New Insights into P Granule Dynamics. Molecular & Cellular Proteomics 15.5; 2016. 1642-1657.
- LeThanh, H.; Neubauer, P.; Hoffmann, F. (2005): The small heat-shock proteins IbpA and IbpB reduce the stress load of recombinant Escherichia coli and delay degradation of inclusion bodies. Microb Cell Fact 4, 6; 2005.
- Martínez-Gómez A.I.; Martínez-Rodríguez S.; Clemente-Jiménez J.M.; Pozo-Dengra J.; Rodríguez-Vico F.; Las Heras-Vázquez F.J. (2007): Recombinant polycistronic structure of hydantoinase process genes in Escherichia coli for the production of optically pure D-amino acids. Appl Environ Microbiol. 73(5); 2007. 1525-1531.
- Kotowska M.; Pawlik K.; Smulczyk-Krawczyszyn A.; Bartosz-Bechowski H.; Kuczek K. (2009): Type II Thioesterase ScoT, Associated with Streptomyces coelicolor A3(2) Modular Polyketide Synthase Cpk, Hydrolyzes Acyl Residues and Has a Preference for Propionate. Appl Environ Microbiol. 75(4); 2009. 887-896.
Ngaahi mo'oni'i me'a 'oku mahu'inga ke 'ilo'i
Ko e ha ʻa microtiter ʻu lauʻi peleti?
ʻOku toe ʻiloa foki ʻa e ʻu lauʻi peleti Microtiter, ʻa ia ʻoku toe ʻiloa ko e ʻu lauʻi peleti lelei ʻe 96, 96 microplates, ʻu lauʻi peleti fakavahaʻa-taʻu pe ngaahi peleti ʻa ʻELISA, ʻa ia ʻoku fakaʻaongaʻi lahi ʻi he laboratories ke ngaue ki he ngaahi selo, sipinga mopaila. ʻOku angamaheni ʻaki hono fakaʻaongaʻi ʻa e ʻu lauʻi peleti Microtiter ʻi manifold ngaahi lea hange ko ʻELISA mo PCR. Ko e lahi taha ʻo e ngaahi peleti lelei ʻe 96 ʻoku fakataumuʻa ia ki he throuput maʻolunga workflows pea ʻoku fenapasi ia mo ngaahi meʻalea ngaahi meʻa mo e ngaue angamaheni mo e ngaue. Ko ia ʻoku lahi ai e fakakeheʻi hange ko skirted ʻu lauʻi peleti ʻoku feʻunga mo e ngaahi platforms angamaheni, ʻikai skirted, Semi skirted, mo e ngaahi lauʻi peleti ʻoku liliu ki he sipinga ʻo e ngaue ʻoku lava ke maʻu.
ʻOku kau ʻi hono fakaʻaongaʻi angamaheni ʻo e ʻu lauʻi peleti microtiter ʻa e ngaahi founga fakaʻaongaʻi hange ko e sipinga ʻo e tanaki, teuteu komipauni, combinatorial e kemisitulii, sivi maʻolunga throughput, nucleic fakamaʻa ʻo e ʻesiti, hangatamaki tupulaki fakafonua, mo e peleti replication.