Ultrasonic Lysis ʻo Drosophila melanogaster sipinga

ʻOku fakaʻaongaʻi lahi ʻa e Drosophila melanogaster ʻi laboratories ko ha sipinga ʻo e motolo. Ko ia ai, ko e ngaahi sitepu teuteu ngaahi meʻa hange ko e lysis, fakahohaʻasi toʻotoʻo, ivi toʻo hingoa mo e DNA ʻo e Drosophila melanogaster kuo pau ke faʻa fakahoko maʻu pe. ʻOku falalaʻanga mo lelei ʻa ultrasonic dismembrators pea ʻe lava ke fakaʻaongaʻi ia ke fakahoko ʻaki ha ngaahi ngaue kehekehe hange ko e lysis, ivi toʻo ʻuli pe DNA fragmentation ʻi he faingofua pe hono liliu ultrasonic ngaahi fakangatangata ki he founga ngaue. ʻOku ultrasonic homogenizers ʻa e ngaahi meʻangaue ʻoku ala fulifulihi holo ʻaki ha ngaahi founga kehekehe.

Ultrasonic Lysis mo e ivi toʻo hingoa

Drosophila melanogaster is widely used as model organism in biological labs. Find here protocols for lysis, protein extracion and DNA shearing of D. melanogaster samples.Ko e Lysis, solubilization toʻotoʻo, homogenization mo e ivi toʻo ʻuli ko ha ngaahi ngaue angamaheni ia ki ultrasonic dismembrators ʻi he laboratories totonu. ʻOku feʻunga lelei ʻa e ultrasonic dismembrators mo e disruptors toʻotoʻo ki homogenize pepa holoholo ʻo e monumanu, ʻinisekite (e.g., Drosophila melanogaster, C. elegans) pe to ngaahi mataʻitohi. Ko hono fakaʻaongaʻi ko ia ʻo e ultrasonication ko e lysis ia ʻo e suspensions toʻotoʻo mo e pellets pea pehe ki hono toʻo ʻo e intracellular polotini.
Ko e ultrasonic lysis mo e ivi toʻo ʻuli ko ha founga falalaʻanga mo reproducible ia, ʻa ia ʻe lava ke fakahoko ʻo makatuʻunga ʻi he ngaahi founga kuo fokotuʻu. Koeʻuhi ʻe lava ke fakatonutonu ʻa e malohi ʻo e ultrasonic ʻo fakafou ʻi he ngaahi fakangatangata sonication hange ko e amplitude, siakale/sepo, mafana mo e sipinga ʻo e tohi, ʻe lava pe ke toutou fakahoko ʻaki ʻa e ola tatau.

Ngaahi lelei ʻo ultrasonic sipinga ʻo e teuteu

  • lelei 'aupito
  • Adjustable ki ha sipinga pau ʻo e naunau
  • Feʻunga mo ha faʻahinga tohi pe
  • Faitoʻo ʻoku ʻikai valaloto
  • ngaahi ola 'o e ngaahi
  • Faingofua mo malu

Ultrasonic DNA mo E RNA Fragmentation

Hili hono toʻo e lysis toʻotoʻo mo e ivi toʻo ʻuli, ko ha sitepu angamaheni ia ʻoku fie maʻu ʻi he sipinga ʻo e teuteu ko e fekau mo e fragmentation ʻo e DNA, RNA, mo e chromatin, e.g. kimuʻa pea chromatin immunoprecipitation (CHIP). ʻE lava ke reliably aʻusia ʻa e DNA mo e RNA fragmentation ʻaki hano maumauʻi ʻa e ngaahi haʻi covalent ʻoku ne pukepuke fakataha ʻa e DNA ʻe he ngaahi malohi fakatuʻasino. ʻI hono fakaʻaongaʻi ʻo e fulufuluʻi fulufuluʻi fulufuluʻi hange ko e sonication, ʻi he kamataʻanga ʻoku maumau ʻa e ngaahi tuʻoni afo ʻo e DNA, pea ʻoku fragmented leva ʻa e DNA ki ha fanga kiʻi kongokonga iiki ange.
ʻOku falalaʻanga mo lelei ʻa e ultrasonic DNA fragmentation ʻi hono kamataʻi ʻo e DNA ki ha loloa pau, hange ko e 500bp (ngaahi hoa ʻi he fakavaʻe). ʻOku kau ʻi he ngaahi lelei lalahi ʻo ultrasonic DNA fragmentation ʻa hono puleʻi totonu ʻo e ngaahi fakangatangata mo e malohi ʻo e ultrasonic. ʻE lava ke fakatonutonu ʻa e ngaahi fakangatangata ʻo e ultrasonic ʻi hono fakatonutonu sonication malohi, siakale mo e taimi totonu. ʻOku malava heni ke faʻu ha tuʻunga kehekehe ʻo e DNA pea ʻe lava ke reliably faʻu mo hiki ʻa e loloa ʻo e DNA kuo fakataumuʻa ki ai. ʻOku toe lelei foki ultrasonic e fekauʻaki ʻa e DNA ke fakatupu ha kavenga mamafa molecular e DNA.

Ultrasonicator UP200Ht with microtip S26d2 for ultrasonic lysis of Drosophila samples

ultrasonicator UP200Ht mo e 2mm microtip S26d2 ki he sonication ʻo drosophila sipinga

Kole 'a e fakamatala




Fakatokanga'i ange 'etau Tu'utu'uni totonu fakatautaha.


Ultrasonic Lysis ʻo Drosophila melanogaster

Te ke lava ʻo maʻu ʻi lalo ha ngaahi ultrasonically kehekehe ki he lysis ʻoku tokoniʻi ʻe he ultrasonically, ivi toʻo ʻuli, mo e DNA pe chromatin fragmentation ʻo drosophila sipinga.

Ultrasonic Lysis ki hono fakafehokotaki ʻo e kolosi Immunoprecipitation (konga) lea

CLIP assay was performed as previously reported with some modifications. Approximately 20 mg ovaries from 0- to 1-day-old wild-type females were UV crosslinked (3 × 2000 μJ/cm2), homogenized on ice in 1 mL RCB buffer (50 mM HEPES pH 7.4, 200 mM NaCl, 2.5 mM MgCl2, 0.1% Triton X-100, 250 mM sucrose, 1 mM DTT, 1× EDTA-free Complete Protease Inhibitors, 1 mM PMSF) supplemented with 300 U RNAseOUT and placed on ice for 30 min. The homogenate was sonicated on ice, at 80% power, five times in 20 s bursts with a 60 s rest in between using the Hielscher Ultrasonic Processor UP100H (100 W, 30 kHz) mo centrifuged (16000 × g ʻi ha miniti ʻe 5 ʻi he 4 °C). Naʻe ʻosi maʻu ʻa e Soluble toʻo ʻaki ha μl e 20 ʻi he dynabeads ʻi ha miniti ʻe 20 ʻi he 4 °C. Hili hono toʻo e ngaahi sipinga ki he immunoblotting mo e quantitation ʻo e RNA input (1%), hp1 naʻe immunoprecipitated mo e kau ʻAnitai-HP1 9A9 antibody mei he 450 μl kuo ʻosi toʻo ʻe he incubation ki he 4 h mo e 50 μl ivi-Gdynabeads. Naʻe fufulu tuʻo 4 ʻa Immunoprecipitates mo RFB. Ke elute ʻa e immunoprecipitated RNAs, naʻe haka ʻa e pelleted ʻasi ʻi he 100 μL ʻo UltraPure DEPC-vai ʻi ha miniti ʻe 5. 900 μL Qiazol Reagent naʻe tanaki atu ia ki he supernatant teuteu ki he RNA. Naʻe fakaʻaongaʻi ʻa e RNA fakamaʻa ko ha template ke synthesize ʻa e FDNA ʻo fakaʻaongaʻi ʻa e oligo dT, hexamers mo superScript fulihi ʻa e III ʻo fakatatau ki he ngaahi founga ʻa e tokotaha ngaohi kapeti.
(Cassale et Al. 2019)

Ultrasonic Lysis for Chromatin Immunoprecipitation as

Naʻe fakahoko Chromatin immunoprecipitation ʻo fakatatau ki he founga naʻe fakamatalaʻi ʻaki ʻe Heneti ʻaki ha fanga kiʻi liliu iiki. Meimei ko e 20 mg ʻoku kehekehe mei he 0-ki he 1 naʻe homogenized ʻa e kakai fefine taʻu 1 ʻi he 1 mL ʻo NEB ngataʻanga (10 mM HEPES-na ʻi he pH 8.0, 10 mM Nafl, 0.1 mM EGTA-Na ʻi he pH 8, 0.5 mM EDTA-na ʻi he pH 8, 1 mM DTT, 0.5% NP-40, 0.5 mM Spermidine, 0.15 mM Spermine, 1× EDTA-tauʻataina Protease Inhibitors) mo ha homogenizer/immersion disperser 10 (00pm). Naʻe ʻave ʻa e homogenate ki ha ipu sioʻata naʻe ʻikai ke ʻi ai ha paʻanga pea naʻe fakaʻaongaʻi kakato ʻe 15 ha pestle malu. Naʻe centrifuged leva ʻa e nuclei taʻetotongi ʻi 6000xg ʻi ha miniti ʻe 10 ʻi he 4 °C. Naʻe resuspended ʻa e nuclei-pellets ʻi he 1 mL ʻo NEB mo centrifuged ʻi he 20000 × g ʻi ha miniti ʻe 20 ʻi he sucrose gradient (0.65 mL ʻo e 1.6 M sucrose ʻi NEB, 0.35 mL ʻo e 0.8 M. Naʻe resuspended ʻa e pellet ʻi he 1 mL ʻo NEB pea formaldehyde ki ha tokanga fakaʻosi ʻo e 1%. Naʻe fakafehokotaki ʻa Nuclei ʻi ha miniti ʻe 10 ʻi he mafana ʻo e loki pea ne fuʻu fie maʻu ʻi hono tanaki atu ʻo e 1/10 Vol ʻo e 1.375 M glycine. Naʻe tanaki ʻa e nuclei ʻe centrifugation ʻi he 6000 × g ʻi ha miniti ʻe 5. Naʻe fufulu tuʻo ua ʻa Nuclei ʻi he 1 mL ʻo NEB pea resuspended ʻi he 1 mL ʻo Lysis ngataʻanga (15 mM HEPES-na ʻi he pH 7.6, 140 mM Akafl, 0.5 mM EGTA, 1 mM EDTA ʻi he pH 8, 1% Triton X-100, 0.5 mM DTT, 0.1% na Deoxycholate, 0.1% SDS, 0.5% N-lauroylsarcosine mo e 1× EDTA-tauʻataina). Naʻe sonicated Nuclei fakaʻaongaʻi ha Hielscher ultrasonic processor UP100H (100 W, 30 kHz) tuʻo ono ʻi he miniti ʻe 20 mo e miniti ʻe 1 ʻi he ʻaisi. Naʻe centrifuged ʻa Sonicated nuclei ʻi he 13000 × g ʻi ha miniti ʻe 4 ʻi he 4 °C. Ko e konga lahi ʻo e sonicated chromatin ko e 500 ki he 1000 (BP) hono loloa. ʻI he immunoprecipitation takitaha, naʻe incubated ai ha μg ʻe 15 ʻo chromatin ʻi he ʻao ʻo e 10 μg ʻo HP1 9A9 monoclonal antibody (3 h ʻi he 4 ° C ʻi ha veʻeteka vilo). ʻI he taimi ko ia, naʻ μl tanaki atu ʻa e dynabeads ʻe 50 ʻo e ivi ʻo e dynabeads G pea naʻe kei hokohoko atu pe incubation ʻi he po ko ia ʻi he 4 °C. Naʻe liʻaki ʻa e supernatants pea naʻe fufulu tuʻo ua ʻa e ngaahi sipinga ʻi Lysis ngataʻanga (fo takitaha miniti ʻe 15 ʻi he 4 ° C) pea tuʻo ua ʻi he TE ngataʻanga (1 mM EDTA, 10 mM TrisHCl ʻi he pH 8). Naʻe holoki ʻa Chromatin mei he ʻikai ke ʻi ai ha sitepu ʻe ua; ʻuluaki ʻi he 100 μl ʻo e eluition ngataʻanga 1 (10 mM EDTA, 1% SFU, 50 mM TrisHCl ʻi he pH 8) ʻi he 65 ° c ʻi ha miniti ʻe 15, hoko ai mo centrifugation mo fakaakeake ʻa e supernatant. Naʻe toe maʻu ʻa e naunau ne ʻasi ʻ μl 100 ʻo e TE + 0.67% S FUʻU. Naʻe incubated ʻa e eluate (200 μl) ʻi ha po kakato ʻi he 65 ° c ke fulihi ʻa e ngaahi fehokotakiʻanga ki he kolosi pea ngaohi ʻe he 50 μg/ml RNaseA ʻi ha miniti ʻe 15 ʻi he 65 ° c pea mo e 500 μg/ml Proteinase K ki he 3 h °C. Naʻe phenol ʻa e sipinga – chloroform maʻu mo ethanol meʻa--. Naʻe toe nofoʻi ʻa e DNA ʻi ha μl 25 ʻo e vai. Ke maximising ʻa e molecular pehe mo e DNA immunoprecipitated, naʻe toe lahi ange ʻa e kau kanititeiti afa ʻi ha optimized duplex-PCR Protocol ʻaki hano fakaʻaongaʻi ha ongo seti kehekehe ʻe ua ʻo e primers ʻi ha tali pe ʻe taha.
(Fakatau et Al. 2019)

UP200St TD_CupHorn he sonication ʻoku ʻikai fakahangatonu ʻo e ngaahi sipinga

UP200St TD_CupHorn ki he sonication ʻoku ʻikai fakahangatonu ʻo e ngaahi sipinga hange ko e DNA mo e chromatin fulufuluʻi

Ngaue ultrasonic toʻotoʻo Disruptors ki he ngaahi sipinga totonu

Ko Hielscher Ultrasonics ko ho hoa ngaue taimi loloa ia ʻi he taimi ʻoku hoko mai ai ki he ngaue maʻolunga ultrasonicators ki he fakahohaʻasi toʻotoʻo, lysis, ivi toʻo hingoa, DNA, RNA, mo e chromatin fragmentation pea pehe ki he ngaahi sitepu teuteu ki he teuteu ngaahi meʻa. ʻOku maʻu ʻe Hielscher ʻa e meʻangaue lelei taha ultrasonic ki hoʻo tohi kole mo e ngaahi fie maʻu totonu, ʻi hono fai ha lekooti fakaʻauliliki ʻo ultrasonic loki fakatotolo homogenizers mo e sipinga ʻo e ngaahi ʻiuniti teuteu.
Vakili-fa'ahinga insonifier UP200St ki he lysisTalafungani fekumi-taipeʻi ultrasonicator ʻaki ha kiʻi tokoni Micro hange ko e UP200St (200W; vakai ki he fakatata toʻohema) pe ko ha taha ʻo e sipinga ultrasonic sipinga teuteu ʻo e ngaahi ʻiuniti VialTweeter pe UP200ST_TD_CupHorn mo VialHolder ko ha kau faʻifaʻitakiʻanga favourite ʻi he fakatotolo mo e ngaahi meʻa laboratories. ʻOku lelei ʻa e ultrasonic ʻiloa fekumi, ʻi he taimi ʻoku teuteuʻi ai ha ngaahi sipinga siʻisiʻi ange kuo pau ke lysed, maʻu pe fragmented. ʻOku hanga ʻe he sipinga ʻo e ngaahi ʻiuniti teuteu VialTweeter mo UP200St_TD_CupHorn ʻo fakaʻata ʻa e hoko sonication ʻo aʻu ki he 10 pe 5.
Kapau ʻoku lahi ʻa e ngaahi fika (e.g. 96-ʻu lauʻi peleti vai, microtiter ʻu lauʻi peleti mo e ala meʻa pehee), kuo pau ke fai ha ngaue ki ai UIP400MTP Ko e fokotuʻutuʻu sonication lelei taha ia. ʻOku ngaue ʻa e UIP400MTP ʻo hange ha cuphorn lahi ange, ʻa ia ʻoku fonu ʻi he vai pea ʻoku ʻi ai ha feituʻu feʻunga ke maʻu ai ha ʻu lauʻi peleti Micro. ʻI hono fakalele ʻe ha 400 Watts malohi ultrasonic processor, ʻoku fakahoko ʻe he UIP400MTP ha teunga mo ha sonication lahi ʻo e ʻu lauʻi peleti fakavahaʻa-taʻu kae lava ke veuki e ngaahi selo, lyse sipinga, solubilize pellets, toʻo polotini pe kosi DNA.

Mapuleʻi totonu ʻo fakafou ʻi he polokalama Smart Software

Processors ngaue 'a e Hielscher 'o e ngaahi hdT 'e lava ke ongo'i fiemalie mo faka'onga'i fakalele 'o fakafou 'i he browser remote control.ʻOku fakanaunau ʻa e ngaahi founga Hielscher sonication kotoa pe mei he 200 Watts ʻaki ha naunau fakaʻilekitulonika colour ki he monitoa mo ha polokalama fakakomipiuta fakapotopoto. ʻOku fakahaofi he taimi pe ko ia ʻa e ngaahi fakangatangata ʻo e founga ngaue protocolling ultrasonic kotoa pe ʻi he faile CSV ʻi ha kaati SD ʻi he kamata pe ʻa e ultrasonicator. ʻOku hanga ʻe he meʻa ni ʻo ʻai ke toe faingofua ange ʻa e fakatotolo mo e protocolling. Hili e ngaahi ʻahiʻahi sonication pe sipinga teuteu, te ke lava pe ʻo toe vakaiʻi ʻa e ngaahi fakangatangata sonication ʻo e sonication takitaha pea fakafehoanaki kinautolu.
ʻI he menu, ʻe lava ke pisitoni ha parameter lahi kimuʻa pea toki sonication: hange ko ʻeni, ke mapuleʻi e mafana ʻo e ʻea ʻi he sipinga pea taʻofi hono valaloto, ʻe lava ke fokotuʻu ha fakangatangata ʻi ʻolunga ʻo e mafana ʻo e ʻea. ʻOku ʻomi ʻe ha pluggable mafana sensor resistance, ʻa ia ʻoku haʻu fakataha mo e ʻiuniti ultrasonic, ʻa e fakamatala ultrasonic processor ʻi he mafana totonu sonication. ʻI he taimi ʻoku aʻu ai ki he fakangatangata ʻo e ʻea ʻi ʻolunga, ʻoku kiʻi longo ʻa e ultrasonic device kae ʻoua kuo aʻu ki he fakangatangata maʻulalo ʻo e seti ∆T pea toe kamata leva sonicating.
Kapau ʻoku fie maʻu ha sonication mo ha ivi pau, te ke lava ʻo pisitoni ʻa e ivi fakaʻosi ultrasonic ʻo e lele sonication. Ko e moʻoni, ʻe lava ke fokotuʻu fakafoʻituitui pe ʻa ultrasonic pulsation mo e cycle Mode.
Ke toe fakaʻaongaʻi hoʻo ngaahi fakangatangata sonication lelei taha, te ke lava ʻo seivi ʻa e ngaahi founga sonication kehekehe (e.g. sonication taimi, malohi, siakale ʻo e mode mo e ala meʻa pehe) ko ha founga kimuʻa, koeʻuhi ke lava ʻo faingofua pea toe kamata vave.
Ke maʻu ha faingamalie lahi ange, ʻe lava ke fakalele ʻa e ngaahi ʻiuniti ultrasonic kotoa pe ʻo fakafou ʻi he browser ʻoku ʻikai lava ke mapuleʻi ʻi ha faʻahinga browser angamaheni (e.g., InternetExplorer, Safari, Chrome etc.). Ko e fehokotakiʻanga LAN ko ha kiʻi fokotuʻutuʻu faingofua pe ia ʻo e plug-n pea ʻoku ʻikai fie maʻu ke toe fola ha polokalama fakakomipiuta kehe.
ʻOku tau ʻilo ʻi Hielscher ʻoku fie maʻu ʻe he sonication lelei ʻo e ngaahi sipinga totonu ʻa e totonu mo e repeatability. Ko ia ai, naʻa mau fokotuʻutuʻu ʻemau ultrasonicators ko ha ngaahi meʻangaue fakapotopoto mo e ngaahi meʻa kotoa pe ʻe lava ke maʻu ai ha teuteu lelei, falalaʻanga, reproducible mo faingamalie.

Fetuʻutaki mai he taimi ni ʻo fakamatala mai fekauʻaki mo hoʻo ngaahi sipinga totonu mo e ngaahi sitepu ʻoku fie maʻu ki he teuteu. Te mau fokotuʻu atu ʻa e sipinga ultrasonic feʻunga taha ʻo e meʻangaue teuteu mo tokoniʻi koe ʻi ha toe ngaahi fakamatala hange ko e ngaahi fokotuʻu mo e ngaahi fokotuʻu.

ʻOku ʻoatu ʻe he tepile ʻi lalo ha fakaʻilonga ʻo e fakafuofua ki he tuʻunga ʻo ʻetau ngaue ultrasonic mei ultrasonic ngaahi tokoni Micro mo e ultrasonic homogenizers ki MultiSample ultrasonicators ki he teuteu lelei mo falalaʻanga ʻo ha ngaahi sipinga lahi:

Kulupu (batch) 'o e tohi 'Oku tafe mai 'a e 'ea 'Oku fokotu'u atu 'a e ngaahi me'angaue
96-ngaahi lauʻi peleti/microtiter n.a. UIP400MTP
10 à 0.5 ki he 1.5 mL n.a. VialTweeter 'i he UP200St
CupHorn ki he sonication ʻoku ʻikai fakahangatonu, hange ko e 5 n.a. UP200ST_TD_CupHorn
0.01 ki he 250mL 5 ki he 100mL/miniti UP50H
0.01 ki he 500mL 10 ki he 200mL/miniti 'e UP100H
0.02 ki he 1L 20 ki he 400mL/miniti 'e UP200Ht / UP200St
mL 'i he 10 ki he 2000 20 ki he 400mL/miniti 'e UP200Ht, UP400St
0.25 ki 5L 0.05 ki he 1L/miniti UIP500hdT

Fetu'utaki mai kiate kimautolu! / Kole kiate kitautolu!

Kole ki ha fakamatala lahi ange

Kataki 'o ngaue 'aki e foomu 'i lalo ke kole ha toe fakamatala ki he ultrasonic processors, faka'aonga'i mo e totongi. Te mau fiefia ke alea'i e founga 'oku mo 'i ai mo ho'omou ngaahi fie ma'u ultrasonic he polokalama!









Kataki 'o fakatokanga'i ange 'etau Tu'utu'uni totonu fakatautaha.


The VialTweeter is a MultiSample Ultraonicator that allows for reliable sample preparation under precisely controlled temperature conditions.

Ko e ʻiuniti teuteu ultrasonic fakavahaʻa-taʻu VialTweeter fakaʻata ʻa e hoko sonication ʻo aʻu ki ha foʻi vaio ʻe 10. ʻI he clamp ʻa e meʻangaue VialPress, ʻe lava ke lomiʻi ai ha toe tiupi ʻe 4 ki muʻa ʻi ha sonication lahi.

Ngaahi tohi/fakamo'oni fakafolofola



Hielscher Ultrasonics supplies high-performance ultrasonic homogenizers from lab to industrial size.

Ngaue ultrasonics maʻolunga! ʻOku ʻufiʻufi ʻe he koloa ʻa Hielscher ʻa e tuʻunga kakato mei he fale fakatotolo fakakemi ultrasonicator ʻi he ngaahi ʻiuniti ʻi ʻolunga ki he ngaahi polokalama ultrasonic lalahi.


Ngaahi mo'oni'i me'a 'oku mahu'inga ke 'ilo'i

Metabolomics

Ko e Metabolomics ko hono ako ia ʻo e fanga kiʻi molecules iiki, ʻoku ʻiloa ko metabolites, ʻi loto ʻi he ngaahi selo, biofluids, pepa holoholo pe organisms. ʻOku fakamatalaʻi fakanounou ʻa e fanga kiʻi molecules iiki ko ʻeni mo ʻenau ngaahi fengaueʻaki ʻi ha founga totonu ʻi he foʻi lea ko e "metabolome" pea ʻoku ui ʻa e feituʻu ʻo e fekumi ko e metabolomics. ʻOku fehokotaki vaofi ʻa e fakatotolo metabolome mo e feituʻu ʻoku vave ai e faitoʻo. ʻOku tokoni ʻa e mahino ʻo e metabolome mo ʻene fekauʻaki mo e ngaahi mahaki kehekehe ke fakatupulaki ʻa e fakaʻehiʻehi mei he mahaki mo kiliniki ngaahi founga tokangaʻi fakafoʻituitui variability ʻi he ʻatakai, toʻonga moʻui, fakatupu, mo molecular phenotype. Koeʻuhi ke tukuange metabolite molecules mei he ngaahi selo, ʻoku faʻa fakaʻaongaʻi ʻa e ultrasonication ʻi he laboratories totonu ki he sipinga kimuʻa ngaahi meʻa teuteu hange ko e fakahohaʻasi toʻotoʻo, lysis mo hono toʻo e ʻuli ʻo e polotini, lipids mo e molecules kehe.